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Небесная энциклопедия

Космические корабли и станции, автоматические КА и методы их проектирования, бортовые комплексы управления, системы и средства жизнеобеспечения, особенности технологии производства ракетно-космических систем

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Применить Всего найдено 4. Отображено 4.
21-11-2012 дата публикации

Carboxylesterase D-1CarE5 from thermophilic Alicyclobacillus tengchongensis strain, and gene thereof

Номер: CN102787107A
Автор: Huang Zunxi, Xie Zhenrong, Zhou Junpei, Li Junjun, Tang Xianghua, Yang Yunjuan, Xu Bo
Принадлежит:

The invention relates to a carboxylesterase D-1CarE5 having an amino acid sequence represented by SEQ ID NO.1 and a gene thereof, and provides a coding gene D-1CarE5 of the carboxylesterase, a recombinant vector of the carboxylesterase gene D-1CarE5, and a recombinant strain of the carboxylesterase gene D-1CarE5. The carboxylesterase has the following properties: the carboxylesterase has an optimum temperature of 40DEG C and an optimum pH value of 7.38 when alpha-naphthyl acetate is used as a substrate; the carboxylesterase can be activated by metal ions comprising Mn<2+> and Zn<2+> when the final concentrations of the metal ions are 1mM, and can be strongly inhibited by Cu<2+>, Ag<+>, Hg<2+>, Pb<+> and the like; the carboxylesterase keeps basically stable at 35DEG C for 50min; the activity of the carboxylesterase after processed with buffer solutions having a concentration of 1/15mol/L and pH values of 5.91, 7.38 and 8.04 for 80min is above 40%, 90% and 45% respectively; and simultaneously ...

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Номер записи: 1
13-03-2013 дата публикации

Carboxylesterase and application thereof in degradation of pesticides malathion and carbaryl

Номер: CN102965355A
Автор: Huang Zunxi, Xie Zhenrong, Ding Junmei, Zhou Junpei, Li Junjun, Tang Xianghua, Yang Yunjuan, Xu Bo
Принадлежит:

The invention relates to the application of carboxylesterase D-1CarE3, gene D-1CarE3 and carboxylesterase D-1CarE3 in the degradation of pesticides malathion and carbaryl. The amino acid sequence of carboxylesterase D-1CarE3 is as shown in SEQ ID NO.1, the amino acid sequence of the encoding gene D-1CarE3 of the carboxylesterase is as shown in SEQ ID NO.2, and the carboxylesterase comprises recombinant vector of carboxylesterase D-1CarE3 and the recombination strain of the carboxylesterase D-1CarE3. The invention further provides the application of the carboxylesterase D-1CarE3 in the degradation of pesticides malathion and carbaryl. The carboxylesterase has the advantages that the cultivation period is short, the pure protein is easy to obtain, and the malathion can be degraded at high efficiency, and compared with the reported carboxylesterase from insects, the carboxylesterase is higher in efficiency for depredating the pesticide malathion and carbaryl. The invention has an important ...

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Номер записи: 2
11-12-2013 дата публикации

Alkaline thermal-stable esterase K91 Est8 and gene thereof

Номер: CN103436506A
Автор: Huang Zunxi, Ding Junmei, Yu Tingting, Xie Zhenrong, Li Junjun, Zhou Junpei
Принадлежит:

The invention provides an esterase K91 Est8 from Bacillussp.. The invention also provides an encoding gene K91 Est8 of the esterase, and a recombinant vector and a recombination strain thereof. The esterase provided by the invention has the properties of the optimum temperature at 50 DEG C, the optimum pH at 9.0, and the enzyme activity which is kept above 85% and 40% after heat preservation for 60 minutes at 37 DEG C and 50 DEG C, respectively; the esterase is still capable of keeping the activity above 40% after being treated at 37 DEG C for 60 minutes by using borax having the pH of 9.5 and a NaOH buffer solution; the esterase has excellent thermal stability and alkali resistance. Except ethanoic acid-4-nitrobenzene thiocyanate which is the optimum hydrolysis substrate for the esterase, the esterase is further capable of hydrolyzing alpha-naphthyl acetate, beta-naphthyl acetate, 4-nitrobenzene butyrate, 4-nitrophenyl hexanoate and 4-nitrophenyl caprylate; therefore, the esterase can ...

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Номер записи: 3
13-03-2013 дата публикации

Preparation method and application of recombinant carboxylesterase D-1CarE5

Номер: CN102965356A
Автор: Huang Zunxi, Xie Zhenrong, Li Junjun, Ding Junmei, Zhou Junpei, Tang Xianghua, Yang Yunjuan, Xu Bo
Принадлежит:

The invention relates to a preparation method of recombinant carboxylesterase D-1CarE5 and an application of the recombinant carboxylesterase D-1CarE5 in degradation of a pesticide namely malathion. According to the preparation method, an escherichia coli carboxylesterase D-1CarE5 engineering bacteria is subjected to induced expression for 16 hours through alpha-lactose to generate a large amount of soluble recombinant carboxylesterase D-1CarE5 protein, and the soluble recombinant carboxylesterase D-1CarE5 protein is purified by using a Ni<2+>-NTA gravity purification column. Beta-naphthyl acetate is used as a substrate to perform enzymatic activity determination on the property of the recombinant carboxylesterase D-1CarE5. The application method of the recombinant carboxylesterase D-1CarE5 comprises the following steps of: adding 1U (beta-naphthyl acetate is used as the substrate to detect enzymatic activity) of carboxylesterase D-1CarE5 into a disodium hydrogen phosphate-potassium dihydrogen ...

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Номер записи: 4