30-05-2012 дата публикации
Номер: CN0102070646B
Принадлежит:
The invention discloses an extraction method of Diosbulbin B, which comprises the following steps: carrying out reflux extraction on airpotato yam acetone, and concentrating the extracting solution until no acetone smell emits, thereby obtaining an extract; mixing the extract and silica gel, adding petroleum ether for extraction, drying filter residues by evaporation, adding chloroform for extraction, and drying the extracting solution by evaporation, thereby obtaining a chloroform part; mixing the chloroform part and silica gel, passing the mixture through columns, eluting with a mixed solution of chloroform and methanol in the volume ratio of 98:2, and collecting the eluent at multiple sections; recovering the solvent from the eluent, dissolving in acetone, crystallizing to obtain a crystal, passing the filtrate through columns, and eluting with a mixed solution of chloroform and methanol in the volume ratio of 99:1; and recovering the solvent from the eluent, dissolving in acetone, crystallizing ...
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