Method for improving resistance of rice plant to cnaphalocrocis medinalis by transgenic technology

The invention discloses a method for improving the resistance of rice plants to rice case worms by applying the transgenic technology, and belongs to the technical field of the plant gene engineering breeding. The method comprises the following steps: Rubisco specific promoters RubS and insect-resistant genes Cry1A(b) are constructed to a pPZP201 expression plasmid carrier; the Cry1A(b) genes are integrated into a rice genome by using the method of co-transformation mediated by agrobacterium tumefaciens; the homomycin resistance genes in the self-bred progenies are rejected. The Cry1A(b) insect-resistant genes in the transgenic rice present specific high-expression, present low-expression in seeds, and have no expression in roots, thus obviously improving the resistance of the rice plants to the rice case worms, greatly reducing the pesticide application amount, reducing the production cost and improving the plant profit; simultaneously, the expression of the insect-resistant protein in ...

Method for selecting and reproducing early-flowering late japonica rice sterile line Zhe japonica rice 4A with high stigma exposure rate

The invention relates to a method for selecting a sterile line variety of late japonica rice (Dian I type), and particularly discloses a method for breeding and reproducing a high-quality early-flowering late japonica rice sterile line Zhe japonica rice 4A with a high stigma exposure rate. The breeding method comprises the following steps of hybridizing a BT type sterile line 7627A [with the preservation number of CN1793 in the national germplasm repository and ZF02-3033 (combined into Bing 97342//Bing8502/Babawee) (with the preservation number of CGMCCNo.8016), performing back crossing and selfing by using the ZF02-3033 as a male parent, performing back crossing transfer in combination with combining ability determination and high-temperature resistant identification from the BC6F1 generation, and bagging and selfing BC15F1 with the maturing rate of zero to obtain the Zhe japonica rice 4A. The late japonica rice sterile line Zhe japonica rice 4A selected by the method can early flower, ...

Waist bag special for rice breeding

The utility model discloses a special waist bag for rice breeding. The waist bag comprises a waist bag body and waistbands, a fixing sleeve for a waistband to penetrate through is arranged on the inner side of the waist bag body. A scissors bag and a label bag are arranged on the outer side of the waist bag body, the scissors bag comprises a bag body arranged on the waist bag body and a sleeve body arranged above the bag body, the bag body is in an inverted triangle shape, the sleeve body is in an inverted trapezoid shape, and the two side edges of the sleeve body and the corresponding side edges of the bag body are located on the same straight line. And a hole is formed in the outer side of the label bag. By adopting the waist bag, related tools can be effectively collected, sorted and taken, the working efficiency of hybridization and seed selection is improved, and the special tool waist bag is simple in structure, convenient to carry and obvious in effect.

Molecular marking method for detecting rice plant premature aging

The invention relates to a technology for detecting rice variety resource characteristics, in particular to a molecular marking method for detecting a rice premature aging gene. The molecular marking method for detecting rice plant premature aging comprises the following steps of: extracting rice plant DNA; carrying out PCR (Polymerase Chain Reaction) amplification on an extracted DNA template and a specific molecular marker; and detecting a PCR product for judging genotypes of rice plant premature aging characteristics. The specific molecular marker is selected from one or the combination of two of sg1 and sg2, wherein the upstream end of the sg1 is shown as SEQ ID NO:1, and the downstream end of the sg1 is shown as SEQ ID NO:2; the upstream end of the sg2 is shown as SEQ ID NO:3, and the downstream end of the sg2 is shown as SEQ ID NO:4; and three different genotypes of AA, Aa and aa can be detected by combining the sg1 and the sg2. In the invention, the specific molecular markers of ...

Method for improving resistance of rice plant to cnaphalocrocis medinalis by transgenic technology

The invention discloses a method for improving the resistance of rice plants to rice case worms by applying the transgenic technology, and belongs to the technical field of the plant gene engineering breeding. The method comprises the following steps: Rubisco specific promoters RubS and insect-resistant genes Cry1A(b) are constructed to a pPZP201 expression plasmid carrier; the Cry1A(b) genes areintegrated into a rice genome by using the method of co-transformation mediated by agrobacterium tumefaciens; the homomycin resistance genes in the self-bred progenies are rejected. The Cry1A(b) insect-resistant genes in the transgenic rice present specific high-expression, present low-expression in seeds, and have no expression in roots, thus obviously improving the resistance of the rice plantsto the rice case worms, greatly reducing the pesticide application amount, reducing the production cost and improving the plant profit; simultaneously, the expression of the insect-resistant protein in rice ...

Adjustable germination accelerating device for rice breeding

The invention relates to the technical field of breeding, in particular to an adjustable rice breeding germination accelerating device which comprises a shell, a liquid storage barrel is fixedly connected to the middle of the shell, a germination accelerating barrel is arranged in the liquid storage barrel, a plurality of meshes are formed in the outer wall of the germination accelerating barrel, seeds make contact with a water body, the diameter of the meshes is smaller than that of the seeds, and the seeds are prevented from leaking out; a plurality of air outlet assemblies are arranged on the bottom wall of the germination barrel, the bottom of the liquid storage barrel communicates with a plurality of third connectors, the tops of the third connectors communicate with the air outlet assemblies, the bottoms of the third connectors communicate with oxygen supply assemblies, and a heating part is arranged at the bottom of the shell. The purpose of preventing oxygen deficit of the rice ...

Molecular marker for detecting crop premature senility gene and application thereof

The invention provides a molecular marker for detecting a crop premature senility gene and application of the molecular marker, and belongs to the technical field of crop breeding. The molecular marker for detecting the crop premature senility gene provided by the invention can be used for detecting premature senility related genes formed by frameshift mutation due to deletion of four basic groups AACA on the eleventh exon in a coding region of the gene LOCOs03g31550. Research shows that rice containing the premature senility related gene can have a stable premature senility phenotype, leaf tip dry premature senility phenotypes of a second leaf and a third leaf appear in the 10th day after sowing in a field environment, and senility lasts to a mature period. In the tillering stage, the photosynthetic pigment content of mesophyll cells is obviously reduced, the chloroplast structure is changed, light damage is serious, photosynthesis is weakened, and the uric acid content is reduced. The ...

Method for improving the efficiency of the seed propagating and production by using the double-particle mutant

Molecular marking method for detecting rice plant premature aging

The invention relates to a technology for detecting rice variety resource characteristics, in particular to a molecular marking method for detecting a rice premature aging gene. The molecular marking method for detecting rice plant premature aging comprises the following steps of: extracting rice plant DNA; carrying out PCR (Polymerase Chain Reaction) amplification on an extracted DNA template and a specific molecular marker; and detecting a PCR product for judging genotypes of rice plant premature aging characteristics. The specific molecular marker is selected from one or the combination of two of sg1 and sg2, wherein the upstream end of the sg1 is shown as SEQ ID NO:1, and the downstream end of the sg1 is shown as SEQ ID NO:2; the upstream end of the sg2 is shown as SEQ ID NO:3, and the downstream end of the sg2 is shown as SEQ ID NO:4; and three different genotypes of AA, Aa and aa can be detected by combining the sg1 and the sg2. In the invention, the specific molecular markers of ...

Product tracing method and device, electronic equipment and storage medium

The invention relates to a product traceability method and device, electronic equipment and a storage medium, and relates to the technical field of Internet application, and the method comprises the following steps: obtaining product traceability data of a target product; coding the product traceability data based on a preset coding rule to obtain a product data identification code of the target product; and establishing product traceability information of the target product based on the product data identification code and the circulation enterprise identification code. According to the method, the product information corresponding to the industrial product is recorded through the radio frequency identification technology, so that related personnel can conveniently trace the industrial product, and the safety management effectiveness of the industrial product can be improved.

Method for improving the efficiency of the seed propagating and producing by using the double-particle mutant

The invention discloses a method for improving propagation and production efficiency of cross-breeding rice by making use of double-grain mutant, pertaining to the rice breeding technical field. The method comprises the following steps: (1) the breeding of Zhe Rice 22-double grain CGMCC NO.2081, the germplasm resource of rice mutant (Oryza Sativa); (2) the breeding of double-grain male sterile line; (3) the matching and seed production of double-grain male sterile line and restoring line and so on. The invention takes Zhe Rice 22-double grain as parent and produces double-grain male sterile line with the male sterile line or maintenance line of the prior cross-breeding rice, and the double-grain male sterile line then produces male sterile line or F1 generation of cross-breeding rice with double grains with the maintenance line or the restoring line, which reduces more than 20 percent of production cost (including storage and transport) compared with the propagation and seed production ...