07-11-2019 дата публикации
Номер: US20190338321A1
The present invention provides for a genetically modified host cell capable of producing a lactam comprising a 2-pyrrolidone synthase, or an enzymatically active fragment thereof, heterologous to the host cell. 2. The genetically modified host cell of claim 1 , wherein the 2-pyrrolidone synthase comprises an amino acid sequence having at least 70% identity with SEQ ID NO:1 claim 1 , wherein the amino acid sequence comprises one or more of the following conserved amino acid motifs or sites: acyl-activating enzyme (AAE) consensus motif (residues 137 claim 1 , 140-145 claim 1 , and 147-148) claim 1 , acyl-activating enzyme (AAE) consensus motif (residues 140 claim 1 , 257-258 claim 1 , 279-284 claim 1 , 357 claim 1 , 369 claim 1 , 372 claim 1 , 382 claim 1 , and 458) claim 1 , AMP binding site (residues 140 claim 1 , 180-181 claim 1 , 227 claim 1 , 229-230 claim 1 , 233 claim 1 , 257-258 claim 1 , 279-284 claim 1 , 357 claim 1 , 369 claim 1 , 372 claim 1 , 379-382 claim 1 , and 439) claim 1 , and CoA binding site (residues 180 claim 1 , 229-230 claim 1 , 233 claim 1 , 257 claim 1 , 379-381 claim 1 , 433 claim 1 , and 439).3. The genetically modified host cell of claim 2 , wherein host cell is capable of synthesizing Compound 1 or uptaking Compound 1 from the environment or culture.4. The genetically modified host cell of claim 3 , wherein host cell further comprises one or more enzymes of a pathway for synthesizing Compound 1 from a carbon source.5. The genetically modified host cell of claim 4 , wherein pathway for synthesizing Compound 1 from a carbon source that is native to the host cell.6. The genetically modified host cell of claim 4 , wherein pathway for synthesizing Compound 1 from a carbon source is heterologous to the host cell.7. The genetically modified host cell of claim 1 , wherein host cell lacks betaine-CoA ligase.8. The genetically modified host cell of claim 2 , wherein n is an integer from 1 to 20.9. The genetically modified host cell of claim 8 , ...
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