01-04-2021 дата публикации
Номер: US20210095262A1
Автор:
GAO Juan,
HONG Hao,
HUANG Xin,
JAMES Gage,
LI Yanjun,
LIU Fang,
LU Jiangping,
Ma Yulei,
WEI Junlu,
YU Wenyan,
ZHANG Kejian,
ZHANG Na
Принадлежит:
Provided are a proline hydroxylase and uses thereof. The proline hydroxylase comprises having the amino acid sequence of SEQ ID NO: 2 with the exception of a mutation of one or more amino acids; wherein the mutation of one or more amino acids must comprises E27K, and the mutation of one or more amino acids selected from the group consisting of: H14R, L16N, T25R, F26L, E27K, D30S, S33N, E34N, E34G, E34L, E34S, E34D, Y35W, Y35K, S37W, S37F, S37E, S37N, S37T, S37C, W40F, K41E, D54G, H55Q, S57L, I58T, I58Y, I58A, I58R, I58V, I58S, I58C, K86P, T91A, F95Y, C97Y, I98V, K106V, K106T, K106Q, F111S, K112E, K112R, S154A, K162E, L166M, I118F, I118V, I118R, H119R, H119F, I120V, K123D, K123N, K123Q, K123S, K123I, K123T, T130N, D134G, V135K, N165H, D173G, K209R, I223V and S225A, and having proline hydroxylase activity. 1. A proline hydroxylase , comprising a protein having the amino acid sequence of SEQ ID NO: 2 with the exception of a mutation of one or more amino acids; wherein the mutation of one or more amino acids must comprises E27K , and the mutation of one or more amino acids selected from the group consisting of: H14R , L16N , T25R , F26L , E27K , D30S , S33N , E34N , E34G , E34L , E34S , E34D , Y35W , Y35K , S37W , S37F , S37E , S37N , S37T , S37C , W40F , K41E , D54G , H55Q , S57L , I58T , I58Y , I58A , I58R , I58V , I58S , I580 , K86P , T91A , F95Y , C97Y , I98V , K106V , K106T , K106Q , F111S , K112E , K112R , S154A , K162E , L166M , I118F , I118V , I118R , H119R , H119F , I120V , K123D , K123N , K123Q , K123S , K123I , K123T , T130N , D134G , V135K , N165H , D173G , K209R , I223V and S225A , and having proline hydroxylase activity.2. The proline hydroxylase as claimed in claim 1 , wherein the mutation comprises any one of combinations selected from the group consisting of E27K+Y35W/K claim 1 , E27K+K123D/I/Q/S claim 1 , E27K+N165H claim 1 , E27K+S37C/E/F/N/W/T+I223V+K123D/I/Q/S claim 1 , I223V+S37C/E/F/N/W/T+E27K+N165H claim 1 , wherein ‘/’ represents ‘or’.3. A ...
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