Special modified negative pole material for thermal battery and preparation method thereof

The invention discloses a special modified negative pole material for a thermal battery. The special modified negative pole material is powder material comprising LiSi alloy and molten salt eutectoid, the LiSi alloy is 95-98% by the weight, the molten salt eutectoid is 2-5% by weight, and the molten salt eutectoid is in a lithium chloride-potassium bromide-lithium fluoride system. The surface structure of the LiSi alloy is modified by adding the molten salt eutectoid, thereby further enhancing the compatibility between the LiSi alloy and isolation material for the thermal battery, expanding a diffusion channel of lithium ions and further improving the high-power transient output capability of the thermal battery. The special modified negative pole material is mainly applied in the high-power transient output thermal battery. In addition, the invention further discloses a preparation method of the special modified material for the thermal battery.

Construction method of tissue-specific VEGF (vascular endothelial growth factor) expression down-regulation vector

The invention discloses a construction method of a tissue-specific VEGF expression down-regulating vector, and relates to the technical field of gene engineering. The construction method comprises the step of sequentially inserting a tissue-specific promoter and a tetracycline-regulated transcription silence into an expression vector. In order to realize expression down-regulation and function research of VEGF gene tissue specificity, a VEGF gene expression down-regulation vector is constructed by virtue of a tissue-specific promoter. A retina-specific promoter HB sequence and a tetracycline-regulated transcription silence tetR-Krab sequence are sequentially inserted into a mammalian cell expression vector pCAGGS to construct a transcription silence expression vector for specifically down-regulating retina VEGF gene expression, so that tissue-specific VEGF gene expression regulation is realized, and a basis is provided for deep exploration of VEGF gene functions.

Construction method of transgenic mouse capable of expressing four resistant proteins

The invention belongs to the technical field of transgenic animal model construction, and specifically relates to a method capable of rapidly obtaining a transgenic mouse with multiple resistances. The method comprises the following steps: establishing a pInsulator4X-CAG-PHBN carrier, individually subjecting the pInsulator4X-CAG-PHBN carrier and a pInsulator4X-CAG carrier to a restriction endonuclease SwaI linearization treatment, phenol-chloroform extraction, and an ethanol precipitation treatment, then performing transfection of HEK293 cells, and finally injecting the pInsulator4X-CAG-PHBN into pronucleus so as to obtain transgenic mice with four resistances. Only one targeting can obtain transgenic mice with four resistances. The MEF (mouse embryonic fibroblast) with four resistances can be obtained just by maintaining the line of the obtained mouse. The provided novel method can utilize the 2A peptide to obtain transgenic mouse with multiple resistances.

Host cell containing vector for expressing functional recombinant human coagulation factor VII and high-level expression method of functional recombinant human coagulation factor VII

The invention provides a host cell containing a vector for expressing a functional recombinant human coagulation factor VII and a high-level expression method of the functional recombinant human coagulation factor VII, and aims at establishing an expression vector containing F VII, GGCX and VKORC1 recombinant nucleic acids and improving the g-carboxylation modification of the recombinant F VII by virtue of coordinated expression of the GGCX and the VKORC1. The host cell contains the F VII recombinant nucleic acid (human coagulation factor VII), depending on which the vitamin K is coded, a VKORC1 (mouse vitamin K epoxide reductase complex subunit 1) recombinant nucleic acid and a GGCX (mouse g glutamyl carboxylase) recombinant nucleic acid, as well as an insulator (4X) recombinant nucleic acid; the high-level expression method of the functional recombinant human coagulation factor VII comprises the steps of establishing an expression vector, mediating the expression vector into a DHFR deficient ...

Application of EGR-1 (early growth response-1) gene in preparation of medicine for resisting bladder cancer

The invention provides an application of an EGR-1 (early growth response-1) gene in preparation of anti-cancer medicine, and particularly provides an application of the EGR-1 gene in preparation of medicine for resisting the bladder cancer. An over-expression vector of the EGR-1 gene is built to transfect tumor cells, and a result shows that mRNA (messenger ribonucleic acid) of the EGR-1 gene in each transfected tumor cell is remarkably up-regulated, the tumor cells become round, wall attachment performance becomes poor, the cell viability is decreased by 66.2%, and the cells are dead finally. According to the invention, one gene capable of inhibiting the tumor cells efficiently is obtained through screening, and basic data are provided for novel medicine target discovery and antitumor medicine design.

Injection molding vulcanizing machine for automobile rubber product production

The utility model discloses an injection molding vulcanizing machine for automobile rubber product production, which relates to the field of vulcanizing machines and comprises a vulcanizing machine main body, a first air cylinder is mounted on one side of the vulcanizing machine main body, a first clamping jaw is arranged at the output end of the first air cylinder, and a conveyor is arranged at one end of the vulcanizing machine main body. A mounting frame is arranged at the top end of the conveyor. Through the vulcanizing machine main body, the first air cylinder, the first clamping jaw, the conveying machine, the mounting frame, the electric push rod, the push block, the second air cylinder and the second clamping jaw, the second air cylinder belt drives the second clamping jaw to descend during mold opening to clamp raw materials, and then the raw materials are placed into the mold opening under the driving of the electric push rod; then the mold is opened and closed for pressurizing ...

Thermal balance pile used for thermal battery

Steam vulcanizing tank for producing automobile rubber parts

The utility model discloses a steam vulcanizing tank for producing automobile rubber parts, which relates to the field of vulcanizing tanks and comprises a vulcanizing bin, one side of the vulcanizing bin is connected with a waste gas bin, a UV (Ultraviolet) tube is arranged in the waste gas bin, the top end of the waste gas bin is connected with a catalyst feed port, and the other side of the vulcanizing bin is connected with a bin door. A plurality of containing layers are arranged in the vulcanization bin, the bottom ends of the containing layers are connected with a connecting rod, two sides of the connecting rod are connected with two power rods, an electric push rod is fixed to one side of each power rod, a sliding groove is formed in the bottom end of each containing layer, a sliding wheel is connected to the inner wall of each sliding groove, and a U-shaped seat is connected to the bottom end of each sliding wheel. And the bottom end of the vulcanization bin is connected with supporting ...

Duct length measuring device based on sound waves

The invention claims a duct length measuring device, which comprises a logic circuit control part (1), a sound wave emission part (2), a temperature detecting part (3) and a sound wave receiving and signal preprocessing circuit (4). When the device is used for measuring a hollow duct with both open ends, the device can be positioned on one end of the duct for carrying out measurement without any processing on the surface and the ports of the duct. The device has the advantages of simple principle, low cost, strong capacity of resisting disturbance, reasonable structure, light weight, durability and wide application range.

Application of p50 gene in preparation of bladder cancer fighting drug

The invention provides the application of a p50 gene in preparation of cancer fighting drugs, and particularly provides the application of the p50 gene in preparation of a bladder cancer fighting drug. According to the application, transfection of a cancer cell is achieved by establishing an over-expression vector of the p50 gene, and it is indicted by results that, the mRNA of the p50 gene in the cancer cell is greatly increased after transfection; the cancer cell becomes round, the wall attaching performance of the cancer cell becomes poor, the activity of the cancer cell is reduced by 21.8%, and the cancer cell dies finally. According to the application, the gene which can effectively restrain the cancer cell is obtained through screening, and basic data are provided for discovery of novel drug targets and design of tumor drugs.

Novel composite anode material for thermal battery and preparation method thereof

The invention discloses a novel composite anode material for a thermal battery and a preparation method thereof. The anode material is powdery material consisting of the following components in percentage by weight: 80 to 90 percent of FeS2 powder, 10 to 15 percent of ternary electrolyte, 5 to 10 percent of graphite conductive agent and 1.5 to 3 percent of Li2O, wherein the ternary electrolyte is a lithium chloride-lithium bromide-lithium fluoride system. The novel composite anode material greatly improves the voltage output accuracy of the thermal battery under the condition of large load current change, and is mainly applied to the high-power transient output thermal battery. In addition, the invention also discloses a method for preparing the novel composite anode material.

Open mill for producing automobile rubber parts

The utility model discloses an open mill for producing automobile rubber parts, which relates to the field of open mill equipment and comprises an open mill main body, a roller is arranged on the inner side of the open mill main body, a feeding groove is formed in the top end of the open mill main body, a discharging groove is connected to the bottom end of the feeding groove, and a first motor is mounted on one side of the feeding groove. A limiting groove is formed in one end of the feeding groove, a stirring shaft is arranged at the output end of the first motor, and a discharging hole is formed in the bottom end of the discharging groove. By arranging the feeding groove, the first motor, the stirring shaft, the discharging groove and the discharging holes, powder needing to be processed is poured into the feeding groove, the first motor is controlled to operate, the first motor drives the stirring shaft to rotate through the rotating shaft so as to scatter the powder, the scattered ...

Construction and application of GADD45betaRNA interference report vector based on EGFP

The invention relates to construction of a GADD45betaRNA interference report vector based on EGFP and to a method of fast screening siRNA. The method can be used for studying gene function at a cellular level and an in-vivo level. By inserting a GADD45beta open reading frame into a pEGFP-N1 vector, the GADD45betaRNA interference report vector, of p-GADD45beta-EGFP-N1, based on EGFP is constructed, and fusion expression of GADD45beta and EGFP is carried out in the vector. The vector together with the synthetic GADD45betasiRNA cotransfects the bladder cancer cell line T24, and it is found out that interfering effects of GADD45betasiRNA360 and siRNA638 are best. Compared with a conventional qPCR method, the method in the invention is time saving and labor saving. The method is suitable for fast screening siRNA in bladder cancer cells and mammalian cells and is used for studying gene function.

Thermal balance pile used for thermal battery

The invention discloses a thermal balance pile used for a thermal battery, which controls the internal temperature of the thermal battery during working, and balances the heat distribution inside the thermal battery; heat sources at the two ends of the pile and an insulated heat-resisting material are overlapped and tightly assembled at the two ends of the pile, wherein the heat sources at the two ends of the pile consist of iron powder and potassium perchlorate; calculated by weight, the content of the iron powder is 82-88 percents; and the content of the potassium perchlorate is 12-18 contents; and the insulated heat-resisting material is an asbestos product. The thermal balance pile used for the thermal battery not only maintains the internal temperature of the thermal battery in the normal working temperature scope of the pile, but also avoids thermal runaway caused by that the internal temperature of the pile is higher than the thermal decomposition temperature of an anode material ...