caspase-ERLBD fusion gene

The invention discloses a caspase-ERLBD fusion gene, which is connected by a caspase8 gene and a ligand-binding area gene of ER alpha. Fusion protein caspase-ERLBD expressed by the caspase-ERLBD fusion gene can identify estrogens and is specifically combined with the estrogens. By triggering dimerization of the fusion protein caspase-ERLBD, caspase in the fusion protein is activated by interactively cutting molecules, so that the caspase is subjected to molecular cutting so as to be activated, and cell apoptosis is accelerated. Compared with the conventional 'suicide genes' of tumor cells, the caspase-ERLBD fusion gene induces activation and cell apoptosis of the dead molecule caspase by natural smaller molecule estrogens in cells; therefore, the caspase-ERLBD fusion gene does not requireinduction of other exogenous medicaments like other 'suicide gene' systems in the process of introducing and expressing the 'suicide genes'. Therefore, the potential toxic or side effect of the medicament ...

caspase-ERLBD fusion gene

The invention discloses a caspase-ERLBD fusion gene, which is connected by a caspase8 gene and a ligand-binding area gene of ER alpha. Fusion protein caspase-ERLBD expressed by the caspase-ERLBD fusion gene can identify estrogens and is specifically combined with the estrogens. By triggering dimerization of the fusion protein caspase-ERLBD, caspase in the fusion protein is activated by interactively cutting molecules, so that the caspase is subjected to molecular cutting so as to be activated, and cell apoptosis is accelerated. Compared with the conventional 'suicide genes' of tumor cells, the caspase-ERLBD fusion gene induces activation and cell apoptosis of the dead molecule caspase by natural smaller molecule estrogens in cells; therefore, the caspase-ERLBD fusion gene does not require induction of other exogenous medicaments like other 'suicide gene' systems in the process of introducing and expressing the 'suicide genes'. Therefore, the potential toxic or side effect of the medicament ...

Application of miR-494 inhibitor in preparing preparation for resisting bone calcium loss and promoting bone formation

The invention relates to the technical field of biomedical materials, in particular to an application of a small RNA molecular miR-494 inhibitor in preparing a preparation for resisting bone substance loss and promoting bone formation. Proven by tests, the miR-494 acts on an important transcription factor Runx2 gene related to osteoblast differentiation, and inhibits the gene from expressing the corresponding protein, thus inhibiting the osteoblast differentiation, causing bone-formation differentiation disturbance, and resulting in bone substance loss and osteoporosis. The miR-494 inhibitor can decrease the expression abundance of the miR-494, and relieve the inhibiting action of the miR-494 on the bone-formation differentiation, so that the miR-494 inhibitor can be used for preparing the preparation for resisting bone substance loss and promoting bone formation.

Influenza virus hemagglutinin fusion peptide-containing targeted recombinant molecule gene, protein encoded thereby and application thereof

The invention provides an influenza virus hemagglutinin fusion peptide-containing targeted recombinant molecule gene. The influenza virus hemagglutinin fusion peptide-containing targeted recombinant molecule gene is formed by recombining an internalizing single-chain antibody gene, an influenza virus hemagglutinin fusion peptide gene and an apoptotic molecule gene, wherein a gene sequence for encoding a furin protease recognition site is inserted between the internalizing single-chain antibody gene and the influenza virus hemagglutinin fusion peptide gene. A single-chain antibody part of an expression product of the targeted recombinant molecule gene of the kind can identify specificity, combines a target cell expressing a specific antigen and mediates the recombination protein internalization into the endosome of the target cell; the single-chain antibody is separated from the other parts after the recombination protein is cut by the furin protease in the endosome; the hemagglutinin fusion ...

Vanadium suppress agent in use for heavy oil type gas turbine, and preparation method