Common expression vector between 2 subtype receptor of human somatotropin release in hibiting factor and cytosine deaminease of colibacillus, establishment and application

This invention relates to a common expression vector of human somatostatin receptor subtype 2 and coli bacillus cytosine dearminizing enzyme and its set-up and application. Internal ribosome enter site (IRES) is used to connect in series with SSTR-2 and CD to let them express simultaneously to set up new report gene system, which combines with the cancer multiplication suppression mediated by somatostatin receptor, radiation killing, image display and CD to jointly kill cancers.

HBV specificity interference target point gene and its siRNA and application in anti HBV infecting thereof

caspase-ERLBD fusion gene

The invention discloses a caspase-ERLBD fusion gene, which is connected by a caspase8 gene and a ligand-binding area gene of ER alpha. Fusion protein caspase-ERLBD expressed by the caspase-ERLBD fusion gene can identify estrogens and is specifically combined with the estrogens. By triggering dimerization of the fusion protein caspase-ERLBD, caspase in the fusion protein is activated by interactively cutting molecules, so that the caspase is subjected to molecular cutting so as to be activated, and cell apoptosis is accelerated. Compared with the conventional 'suicide genes' of tumor cells, the caspase-ERLBD fusion gene induces activation and cell apoptosis of the dead molecule caspase by natural smaller molecule estrogens in cells; therefore, the caspase-ERLBD fusion gene does not requireinduction of other exogenous medicaments like other 'suicide gene' systems in the process of introducing and expressing the 'suicide genes'. Therefore, the potential toxic or side effect of the medicament ...

Application of microRNA-139

The invention discloses an application of microRNA-139 (miR-139 for short) in preparing a therapeutic drug and diagnostic reagent for metastatic tutors. The miR-139 acts on the chemokine receptor CXCR4 of a target gene to restrain the metastasis and intrusion of tumor cells. In the tumor cells, a human epidermal growth factor family molecule HER2 interacts with CD44 so as to regulate down the expression of the miR-139, therefore, by means of detecting the expressions of the miR-139 as well as the correlative HER2, CD44 and CXCR4, early diagnosis can be carried out on the tumors, and the metastasis and prognosis of the tumors can be evaluated; and by means of expressing the miR-139 in HER2 positive tumors or interfering the upstream signal of the miR-139 to recover the expression of endogenous miR-139, the metastasis of the tumors can be restrained.

caspase-ERLBD fusion gene

The invention discloses a caspase-ERLBD fusion gene, which is connected by a caspase8 gene and a ligand-binding area gene of ER alpha. Fusion protein caspase-ERLBD expressed by the caspase-ERLBD fusion gene can identify estrogens and is specifically combined with the estrogens. By triggering dimerization of the fusion protein caspase-ERLBD, caspase in the fusion protein is activated by interactively cutting molecules, so that the caspase is subjected to molecular cutting so as to be activated, and cell apoptosis is accelerated. Compared with the conventional 'suicide genes' of tumor cells, the caspase-ERLBD fusion gene induces activation and cell apoptosis of the dead molecule caspase by natural smaller molecule estrogens in cells; therefore, the caspase-ERLBD fusion gene does not require induction of other exogenous medicaments like other 'suicide gene' systems in the process of introducing and expressing the 'suicide genes'. Therefore, the potential toxic or side effect of the medicament ...

Prepn and use of reconstituted target cysteine asparagusic enzyme gene

The reconstituted cysteine asparagusic enzyme gene is used to kill tumor cell through injecting the expression vector of the gene into tumor cell to promote the death of tumor cell. The antibody of antineoplastic surface antigen erbB2/HER2, the wiped film structural domain of pseudomonas aeruginosa exotoxin and reconstituted caspase are fused to constitute target reconstitute cysteine asparagusicenzyme. By means of converting patient's peripheral blood T lymphocyte or tumor cell and back transfusion to the patient to produce the said enzyme; local tumor injection of the recombinant adenovirus expression vector; and purifying and injecting target cysteine asparagusic enzyme protein, positive HER2 tumor cell is recognized and killed.

Human anti-HBsAg single-chain antibody/human antibody light chain constant region/protamine truncated recombination gene, coding protein and application

A human anti-HBsAg mono-chain antibody/human antibody light chain constant region/protamine truncate recombinant gene, encoding protein and use are disclosed. The protein has antigen and nucleic acid combined activities. The process is carried out by: combining HBV siRNA with siRNA expression plasmid, transferring to HBV infectious cell specifically, and inhibiting HBV gene expression and copy. It has excellent specificity, efficiency, continuity and safety.

Application of miR-494 inhibitor in preparing preparation for resisting bone calcium loss and promoting bone formation

The invention relates to the technical field of biomedical materials, in particular to an application of a small RNA molecular miR-494 inhibitor in preparing a preparation for resisting bone substance loss and promoting bone formation. Proven by tests, the miR-494 acts on an important transcription factor Runx2 gene related to osteoblast differentiation, and inhibits the gene from expressing the corresponding protein, thus inhibiting the osteoblast differentiation, causing bone-formation differentiation disturbance, and resulting in bone substance loss and osteoporosis. The miR-494 inhibitor can decrease the expression abundance of the miR-494, and relieve the inhibiting action of the miR-494 on the bone-formation differentiation, so that the miR-494 inhibitor can be used for preparing the preparation for resisting bone substance loss and promoting bone formation.

Influenza virus hemagglutinin fusion peptide-containing targeted recombinant molecule gene, protein encoded thereby and application thereof

The invention provides an influenza virus hemagglutinin fusion peptide-containing targeted recombinant molecule gene. The influenza virus hemagglutinin fusion peptide-containing targeted recombinant molecule gene is formed by recombining an internalizing single-chain antibody gene, an influenza virus hemagglutinin fusion peptide gene and an apoptotic molecule gene, wherein a gene sequence for encoding a furin protease recognition site is inserted between the internalizing single-chain antibody gene and the influenza virus hemagglutinin fusion peptide gene. A single-chain antibody part of an expression product of the targeted recombinant molecule gene of the kind can identify specificity, combines a target cell expressing a specific antigen and mediates the recombination protein internalization into the endosome of the target cell; the single-chain antibody is separated from the other parts after the recombination protein is cut by the furin protease in the endosome; the hemagglutinin fusion ...