Method for increasing renaturation yield of denaturation recombination protein

The invention discloses a method for improving the renaturation yield of denaturation recombination proteins. The method relates to that biomacromolecule substances such as polysaccharides(including polyglucose and polysugar such as Ficoll 70, Ficoll 400 and dextran 70) or polyethylene glycols(such as PEG 2000, PEG 3350 and PEG 20000), proteins(such as bovine serum albumins(BSA)) and nucleic acids(DNA or RNA) are prepared to be a mixing macromolecule crowding reagent renaturation buffer solution with a ceterain concentration; because the mixing macromolecule crowding reagent has favorable influences on the renaturation process of proteins, the renaturation yield of proteins are effectively increased. The method disclosed by the invention has the advantages of simple and convenient operation and relatively low price of material reagents, and can obviously improve the renaturation yield of denaturation recombination proteins and acquire proteins with biological activity; therefore, the method ...

Cloth bag dust remover

The utility model relates to an environmental protection equipment technical field, specific cloth bag dust remover that says so, the device include support and protection casing, are provided with air inlet and gas outlet on the lateral wall of protective housing sinistron right side both sides, and the upper end of protection casing is provided with the air outlet, and the lower extreme of protection casing is provided with the flue -dust retainer, the internal air -purifying chamber that is provided with of protective housing, and the filter bag links to each other with the air -purifying chamber, its characterized in that still includes the filter aid, the filter aid include feeding fan, motor, feed bin, go up glassware, conveyer pipe, control valve, the control valve be provided the discharge end of feed bin, the motor links to each other with the control valve, the upper end of feed bin be provided with the glassware, the feed bin discharge gate links to each other with the conveyer ...

Method and equipment for detecting interaction of biomacromolecules

Method and apparatus for screening G protein coupled receptor medicine

This invention shows a method and device which is used to screen G-protein-coupled receptor medicine. First put buffer solution into liquid storage pool.Second, G-protein-coupled receptor exists in organic bodies, it delivers signals to lower effect systems. Arrestin protein can uncouple acceptor and G protein, it helps acceptor to put up endocytosis reaction leaded by coated veside. Third, put G-protein-coupled receptor, medicine molecule, arrestin, G protein into liquid storage pool. Fourth, regulate voltage to make samples to move in one direction. Fifth, change voltage, detect the signals of the samples and screen the medicine molecule. This device includes sample mix-reaction stream, sample separate and detecting stream. This method is very convenient for use. The device has a simple structure and is low in cost.