Preparation method of influenza virus vaccine

The invention discloses a preparation method of influenza virus vaccine and belongs to the technical field of vaccine preparation. The method comprises the steps that: firstly, mammal cells are subjected to monocloning, a monoclonal cell bank is built, then, monoclonal cell strains adapted by influenza viruses are sieved, next, the virus adaptability cell strain virus culture condition is optimized, finally, virus adaptability cell strains are cultured in a bioreactor, influenza viruses are inoculated when the cells grow to a certain density, the viruses are cultured through referring to the optimized virus culture condition, the viruses are subjected to concentration, purification, inactivation and cracking after being harvested, the hemagglutinin content is measured, and the influenza virus vaccine is prepared. The method disclosed by the invention has the advantages that the virus yield is greatly improved, and safety, validity and stability are realized.