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Небесная энциклопедия

Космические корабли и станции, автоматические КА и методы их проектирования, бортовые комплексы управления, системы и средства жизнеобеспечения, особенности технологии производства ракетно-космических систем

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Мониторинг СМИ

Мониторинг СМИ и социальных сетей. Сканирование интернета, новостных сайтов, специализированных контентных площадок на базе мессенджеров. Гибкие настройки фильтров и первоначальных источников.

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Форма поиска

Поддерживает ввод нескольких поисковых фраз (по одной на строку). При поиске обеспечивает поддержку морфологии русского и английского языка
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Применить Всего найдено 2247. Отображено 100.
23-02-2012 дата публикации

Measurement method utilizing internal standard substance

Номер: US20120043207A1
Принадлежит: Wako Pure Chemical Industries Ltd

A subject of the present invention is to provide a measurement method using an internal standard substance in an electrophoresis where an analyte is a protein or a compound. The present invention relates to a measurement method for an analyte by an electrophoresis, characterized in that a peak of the analyte is identified by using as an internal standard substance (1) a combination of a compound I having 3 or more anion groups in a molecule and a compound II where 1 to 3 groups of the anion groups of said compound I have been substituted by cation groups, or (2) a combination of a compound III having 3 or more cation groups in a molecule and a compound IV where 1 to 3 groups of the cation groups of said compound III have been substituted by cation groups.

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04-04-2013 дата публикации

Electrodeionization Method And Device With Hydrodynamic Flow Splitting

Номер: US20130081948A1
Автор: Grabowski Andrej
Принадлежит: EMD MILLIPORE CORPORATION

Method for the removal of ions and ionizable substances from a polar liquid () comprising at least one process wherein said polar liquid () is split into a first stream (F) and a second stream (F), 1. A method for removing ions from a polar liquid , comprising:providing a device comprising at least one housing containing at least one compartment and in which an electrode and a counter-electrode are located at two different ends of the device, and containing an ion-exchange membrane, wherein an electrochemically regenerable ion-exchange material is placed between the electrode and the counter-electrode, the housing comprising one inlet for an entering liquid flow, said inlet being located at the electrode end of the device, and an outlet for a deionized liquid, said outlet being located at the counter-electrode end of the device or at an ion-exchange membrane end of the device, said ion-exchange membrane being a bipolar membrane and being placed between the electrodes, wherein the device comprises valves for splitting the entering liquid flow and at least a second outlet for a liquid used to rinse the electrode, said second outlet being located at the electrode end of the device;splitting said polar liquid into first and second streams;flowing said first stream through said electrochemically regenerable ion-exchange material, causing ions to migrate in the direction reverse to the flow of said first stream through said electrochemically regenerable ion-exchange material; andrinsing one of said electrodes or said ion-exchange material with said second stream.2. The method of claim 1 , further comprising regenerating said ion-exchange material with the ions formed at said counter-electrode or said ion-exchange membrane.3. The method of claim 1 , wherein said second stream rinses one of said electrodes by passing tangentially to said electrode or through said electrode.4. The method of claim 1 , further comprising regulating the flow of said first stream out of said ...

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11-04-2013 дата публикации

Device for varying wetting properties of droplet and device for separating particles using the same

Номер: US20130087459A1

Provided are a device for varying wetting properties and a device for separating particles using the same. The magnitude and switching period of a voltage applied to a droplet are changed to separate particles contained in the droplet based on mass or size. The device for varying wetting properties includes an electrode layer that is electrically conductive with one of different electrical polarities, an insulating layer disposed at one side of the electrode layer, and a droplet that is in contact with the other polarity and contains different particles desired to be separated. Wetting properties of the droplet are varied according to application of a voltage.

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25-04-2013 дата публикации

Photoelectrochemical Synthesis of High Density Combinatorial Polymer Arrays

Номер: US20130098771A1
Принадлежит: Massachusetts Institute of Technology

In a method for creating polymer arrays through photoelectrochemically modulated acid/base/radical generation for combinatorial synthesis, electrochemical synthesis is guided by a spatially modulated light source striking a semiconductor in an electrolyte solution. A substrate having at its surface at least one photoelectrode that is proximate to at least one molecule bearing at least one chemical functional group is provided, along with a reagent-generating chemistry co-localized with the chemical functional group and capable of generating reagents when subjected to a potential above a threshold. An input potential is then applied to the photoelectrode that exceeds the threshold in the presence of light and that does not exceed the threshold in the absence of light, causing the transfer of electrons to or from the substrate, and creating a patterned substrate. The process is repeated until a polymer array of desired size is created. 1. A method for photoelectrochemical synthesis of a biomolecule array , comprising the steps of:(a) providing a semiconductor substrate having at least one light-addressable photoelectrode proximate to the substrate surface;(b) providing an photoelectrochemical reaction-generating chemistry that is in contact with the semiconductor substrate and is capable of generating reagents when subjected to a potential above a threshold, the photoelectrochemical reaction-generating chemistry comprising an electrolyte solution, matrix, gel, or solid that is suitable for photoelectrochemical reactions at a surface;(c) applying an input potential to the light-addressable photoelectrode to generate charge carriers in areas of the substrate under illumination and thereby create a patterned substrate, the applied input potential exceeding the threshold in the presence of light and not exceeding the threshold in the absence of light, the input potential being generated by light from a spatially-modulated light source, the light being patterned by a mask, ...

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02-05-2013 дата публикации

HIGH SPEED, HIGH RESOLUTION COMPOSITIONS, METHODS AND KITS FOR CAPILLARY ELECTROPHORESIS

Номер: US20130105314A1
Принадлежит: APPLIED BIOSYSTEMS, LLC

The invention provides compositions, methods and kits for high speed, high resolution of analytes by capillary electrophoresis starting with uncoated capillaries. The compositions comprise a sieving component, comprising a non-crosslinked acrylamide polymer, and a surface interaction component, comprising at least one uncharged and non-crosslinked water-soluble silica-adsorbing polymer. Methods for employing the novel compositions in capillary electrophoresis are provided. Kits comprising the novel compositions for use in the novel methods are also provided. 156-. (canceled)57. A composition for separating analytes by capillary electrophoresis comprising:a sieving component comprising a non-crosslinked hydrophilic acrylamide polymer having a molecular weight (Mw) greater than about 3,000,000 Daltons (Da) and less than or equal to about 6,400,000 Da; and{'sub': 1', '3', '1', '3', '1', '3', '1', '3, 'a surface interaction component comprising one or more non-crosslinked hydrophobic polymers selected from the group consisting of poly(meth)acrylamide, N,N-disubstituted polyacrylamide and N-substituted polyacrylamide, wherein said N-substituents are selected from the group consisting of Cto Calkyl, halo-substituted Cto Calkyl, methoxy-substituted Cto Calkyl, and hydroxyl-substituted Cto Calkyl;'}wherein:the sieving component and the surface interaction component differ in polymer chemical composition;the composition has a viscosity of less than 10,000 centipoise at 25° C.; andthe composition does not include a crosslinked polymer gel.58. The composition of claim 57 , wherein the one or more non-crosslinked polymers comprises poly(N claim 57 ,N-dimethylacrylamide) as a surface interaction component.59. The composition of claim 57 , further comprising at least one denaturant wherein the denaturant is selected from the group consisting of at least one of formamide claim 57 , urea claim 57 , and 2-pyrollidinone.60. The composition of wherein the composition is configured to ...

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02-05-2013 дата публикации

HIGH SPEED, HIGH RESOLUTION COMPOSITIONS, METHODS AND KITS FOR CAPILLARY ELECTROPHORESIS

Номер: US20130105315A1
Принадлежит: APPLIED BIOSYSTEMS, LLC.

The invention provides compositions, methods and kits for high speed, high resolution of analytes by capillary electrophoresis starting with uncoated capillaries. The compositions comprise a sieving component, comprising a non-crosslinked acrylamide polymer, and a surface interaction component, comprising at least one uncharged and non-crosslinked water-soluble silica-adsorbing polymer. Methods for employing the novel compositions in capillary electrophoresis are provided. Kits comprising the novel compositions for use in the novel methods are also provided. 156-. (canceled)57. A method for separating analytes by capillary electrophoresis comprising: (a) a sieving component comprising an acrylamide polymer having a molecular weight greater than about 3,000,000 Da and less than or equal to about 6,400,000 Da; and', {'sub': 1', '3', '1', '3', '1', '3', '1', '3, '(b) a surface interaction component comprising one or more polymers selected from the group consisting of poly(meth)acrylamide, N,N-disubstituted polyacrylamide and N-substituted polyacrylamide, wherein said N-substituents are selected from the group consisting of Cto Calkyl, halo-substituted Cto Calkyl, methoxy-substituted Cto Calkyl, and hydroxyl-substituted Cto Calkyl;'}], 'separating the analytes by capillary electrophoresis in a composition comprising the sieving component and the surface interaction component differ in polymer chemical composition; and', 'the composition does not include a crosslinked polymer gel., 'wherein58. The method of claim 57 , which is carried out in parallel with a plurality of uncoated capillaries.59. The method of claim 57 , wherein the composition has a viscosity of less than about 10 claim 57 ,000 centipoise at 25° C.60. The method of claim 57 , wherein the composition further comprises at least one denaturant.61. The method of claim 60 , wherein the at least one denaturant is selected from the group consisting of at least one of formamide claim 60 , urea and 2-pyrollidinone ...

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13-06-2013 дата публикации

ARTIFICIAL MYCOLIC ACID MEMBRANES

Номер: US20130146456A1
Принадлежит: UNIVERSITY OF WASHINGTON

Provided herein are artificial membranes of mycolic acids. The membranes may be unsupported or tethered. These membranes are long lived and highly resistant to electroporation, demonstrating their general strength. The mycolic acid membranes are suitable for controlled studies of the mycobacterial outer membrane and can be used in other experiments, such as nanopore analyte translocation experiments. 1. An artificial membrane comprising a mycolic acid.2. The membrane of claim 1 , wherein the mycolic acid is a crosslinked mycolic acid.3. The membrane of further comprising a nanopore.4. The membrane of claim 3 , wherein the nanopore is a protein pore.5Mycobacterium smegmatis. The membrane of claim 3 , wherein the nanopore is α-hemolysin or a variant thereof claim 3 , a porn (Msp) or a variant thereof claim 3 , or OmpATb.6. The membrane of claim 3 , wherein the nanopore is a mutant MspA.7. The membrane of claim 6 , wherein amino acids at positions 90 claim 6 , 91 claim 6 , and 93 of the mutant MspA are each substituted with asparagine.8. The membrane of claim 1 , wherein the membrane is an unsupported membrane.9. The membrane of claim 1 , wherein the membrane is a tethered membrane.10. A system comprising an artificial membrane of positioned between a first liquid conductive medium and a second liquid conductive medium.11. The system of claim 10 , wherein the membrane further comprises a nanopore.12. The system of claim 11 , wherein at least one liquid conductive medium comprises an analyte.13. The system of claim 12 , wherein the analyte is a nucleic acid or a protein.14Mycobacterium smegmatis. The system of claim 11 , wherein the nanopore is α-hemolysin or a variant thereof claim 11 , a porin (Msp) or a variant thereof claim 11 , or OmpATb.15. The system of claim 11 , wherein the nanopore is a mutant MspA.16. A method for detecting an analyte comprising:{'claim-ref': {'@idref': 'CLM-00011', 'claim 11'}, '(a) applying an electric field to a system of , sufficient to ...

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11-07-2013 дата публикации

NANO AND MICROFLUIDIC DEVICE FOR SEPARATING AND CONCENTRATING PARTICLES PRESENT IN A FLUID

Номер: US20130175171A1

A device for separating and concentrating particles present in a fluid, including: a first microchannel, having at least one first aperture; and at least one second microchannel, having at least one second aperture, and an end is disclosed. The first microchannel surrounds part or all of the second microchannel at the end. The first microchannel and the second microchannel are connected, at the end, by at least one nanochannel, the nanochannel(s) forming a restriction between the first microchannel and the second microchannel. A cap bounds the first microchannel, the second microchannel and the nanochannel at the end. The first microchannel and the second microchannel are made in a first substrate. The first aperture and the second aperture open into a same face of this substrate. The device may be used for separating and concentrating particles of biological samples, such as viruses, DNA or synthesic molecules. 1. A device for separating and concentrating particles present in a fluid , comprising:a first microchannel, having at least one first aperture;at least one second microchannel, having at least one second aperture, and an end;the first microchannel surrounding at least part of the second microchannel at said end;the first microchannel and the second microchannel are connected, at said end, by at least one nanochannel, the nanochannel(s) forming a restriction between the first microchannel and the second microchannel, the geometry of which is fixed during a predetermined time period;a cap bounding the first microchannel, the second microchannel and the nanochannel at said end, the first microchannel and the second microchannel being made in a first substrate, said first aperture and said second aperture opening into a same face of this substrate.2. The device according to claim 1 , wherein the cap is made in a second substrate claim 1 , assembled with the first substrate claim 1 , the nanochannel being bounded by a space between the first and second ...

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18-07-2013 дата публикации

CAPILLARY ELECTROPHORESIS METHOD FOR ANALYZING COLLAGEN

Номер: US20130180855A1
Автор: Huang Lynn Ling-Huei
Принадлежит: NATIONAL CHENG KUNG UNIVERSITY

A capillary electrophoresis method for identification and analyzing collagen quantitatively, which is used to identify and quantify collagen in a sample, comprises the steps of: (a) dissolving a collagen-containing sample to form a sample solution; (b) preparing a capillary with an inner wall thereof having a positively-charged layer; (c) introducing the sample solution into the capillary filled with an analytical buffer solution; and (d) driving the sample solution to pass through the capillary. The method of the present invention does not need the purifying pre-treatment and cracking the collagen-containing sample but directly performs the capillary electrophoresis analysis of collagen. Therefore, the present invention can shorten the time for analyzing collagen quantitatively. 1. A capillary electrophoresis method for analyzing collagen without cracking the collagen into polypeptides , which is used to identify collagen and quantify the total amount of collagen in a sample , comprising the steps of:dissolving a collagen-containing sample to form a sample solution;preparing a capillary with an inner wall thereof already pre-coated a positively-charged layer;introducing said sample solution into said capillary having an analytical buffer solution;driving said sample solution to pass through said capillary; andhaving collagen peak in a single peak in a resulted chromatogram, thus the collagen can be identified and accurately quantitated.2. The capillary electrophoresis method for identification and analyzing collagen quantitatively according to claim 1 , wherein said analytical buffer solution has a pH between 0.5˜7.0.3. The capillary electrophoresis method for identification and analyzing collagen quantitatively according to claim 1 , wherein said analytical buffer solution has a pH between 2˜3.4. The capillary electrophoresis method for identification and analyzing collagen quantitatively according to claim 1 , wherein said analytical buffer solution is selected ...

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01-08-2013 дата публикации

SYSTEM AND METHOD FOR THE CONCENTRATION OF A SLURRY

Номер: US20130192991A1
Принадлежит:

The present invention relates to a system and a method for the concentration of slurry, especially mineral containing slurry. The invention provides a system comprising an electrophoresis unit and a separation unit, where the separation unit comprises a recipient, preferably of half cylindrical form, with adjusted flanks for separating the solid material or cake from the rotating anodes and a sliding carriage suitable for closing the recipient and stripping resting solid material from the flanks into the recipient before the solid material or cake is pressed out of the recipient by a piston. 1. Device for the concentration of a slurry , with a supporting structure for receiving modules therein , the modules comprising{'b': '50', 'a. an electrophoresis cell with at least one electrically connected cathode, and at least one electrically connected, rotatable anode disc (),'}{'b': 40', '20', '30', '40', '20', '40, 'b. separation units adjacent to each anode surface for receiving the cake material, comprising a recipient () and a piston (), wherein the shoulders () of the recipient () are dimensioned to act as scraping flange for taking off the solid material or cake from the anodes and the piston () for pressing the collected material or cake out of the recipient (),'}c. means for turning the anodes, circulating slurry in and out of the electrophoresis cell, and applying voltage to the electrodes.210403040. Device according to claim 1 , wherein the separation unit has a sliding carriage () with a cover for closing the recipient () for collecting residual material from the shoulders () into the recipient () while closing it.360. Device according to claim 1 , wherein the electrophoresis cell is a multiple compartment container ().4606060. Device according to claim 3 , wherein the multiple compartment slurry container () is made from an outer container () shell with flanges fixed inside the container () to form the compartments.560. Device according to claim 4 , wherein ...

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08-08-2013 дата публикации

METHOD AND SYSTEM FOR DOCUMENTING AN ELECTROPHORESIS GEL

Номер: US20130199930A1

Disclosed in this specification is a high capacity compact gel documentation system for documenting different types of electrophoresis gels or other translucent objects using ultraviolet light. The system includes a base with a scanning surface having a transparent bottom surface. A light source is connected to a conveying mechanism, disposed below the transparent bottom surface, to move the light source over the length of the transparent bottom surface. An image capture device receives a reflected image and provides it to a microprocessor. Moreover, separate interchangeable filters allow for documentation of UV and white light gel captures, alleviating the need for separate transilluminators and hoods with filters and cameras. 1. A gel documentation system for documenting an electrophoresis gel , the system comprising:a base with a scanning surface comprising a transparent bottom surface with a length and a width;a light source operatively connected to a conveying mechanism to move the light source over the length of the transparent bottom surface, the conveying mechanism being disposed below the transparent bottom surface, wherein the light source emits a narrowband wavelength of ultraviolet light;an image capture device for receiving a reflected image;a microprocessor operatively connected to the image capture device.2. The gel documentation system as recited in claim 1 , wherein the light source emits a narrowband wavelength of 254 nm.3. The gel documentation system as recited in claim 1 , wherein the narrowband wavelength of ultraviot light is selected from the group consisting of 254 nm claim 1 , 312 nm claim 1 , 365 nm and combinations thereof.4. The gel documentation system as recited in claim 1 , wherein the base comprises a slot and a sliding filter is inserted into the slot such that the sliding filter is disposed between the light source and the transparent bottom surface.5. The gel documentation system as recited in claim 4 , wherein the sliding filter ...

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29-08-2013 дата публикации

AIR PURIFICATION

Номер: US20130224079A1
Автор: Kusuura Takahisa
Принадлежит: EMPIRE TECHNOLOGY DEVELOPMENT LLC

The present disclosure relates to purification and/or sterilization techniques and devices. Methods and systems are provided herein for removing contaminants from air using a combination of an ionic liquid and a reactive oxygen species. 1. (canceled)2. (canceled)3. (canceled)4. (canceled)5. (canceled)6. (canceled)7. (canceled)8. (canceled)9. (canceled)10. (canceled)11. (canceled)12. (canceled)13. (canceled)14. A kit comprising:a wet filter comprising a porous member, wherein pores in the porous member start on a first surface of the wet filter and pass through the porous member to a second surface of the wet filter;an ionic liquid; anda reactive oxygen species generator.15. The kit of claim 14 , wherein the first surface is opposite the second surface.16. The kit of claim 14 , wherein the wet filter contains the ionic liquid.17. The kit of claim 16 , wherein the ionic liquid comprises: acetonitrile (MeCN) claim 16 , dimethyl formamide (DMF) claim 16 , dimethyl sulfoxide (DMSO) claim 16 , quaternary ammonium claim 16 , compounds comprising an imidazolium group claim 16 , or any combination thereof.18. The kit of claim 14 , wherein the reactive oxygen species generator comprises a source of ultraviolet light.19. The kit of claim 14 , wherein the reactive oxygen species generator comprises a first electrode and a second electrode.20. The kit of claim 19 , wherein the first electrode and the second electrode are attached to the wet filter.21. The kit of claim 20 , wherein the first and second electrodes are in fluid communication with the wet filter.22. The kit of claim 14 , further comprising an air circulator configured to assist air from a first side of the wet filter to a second side of the wet filter.23. A filter cartridge comprising:an anode;a cathode;a porous member, wherein the porous member comprises pores that pass from a front surface of the porous member to a back surface of the porous member so as to allow air to flow from the front surface through and out ...

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31-10-2013 дата публикации

REVERSIBLE CURRENT GEL ELECTROPHORESIS DEVICE FOR SEPARATING BIOLOGICAL MACROMOLECULES

Номер: US20130284599A1
Принадлежит: OREGON HEALTH & SCIENCE UNIVERSITY

Cassette bodies for use with electrophoresis apparatus can be formed of a single piece of molded or machined plastic. Such cassette bodies can include a plurality of channels that pass through the cassette body, from a proximal end to a distal end. Such channels can be defined by upper and lower chambers. The upper chambers can be in fluid communication with a first buffer pool through a semi-permeable membrane, and the lower chambers can be in fluid communication with a second buffer pool. An electric current can be passed through the first and second buffer pools, and then reversed, to perform an electrophoresis operation that can separate a biomolecule of interest from free probes, and provide for convenient collection of said biomolecule of interest. 1. A cassette for use with an electrophoresis device , comprising:a proximal end and a distal end opposite the proximal end;a front face and a back face;at least one chamber defined between the front face and the back face, the chamber having an upper portion and a lower portion, wherein the upper portion includes a proximal opening at or near the proximal end of the cassette and the lower portion includes a distal opening at or near the distal end of the cassette, and wherein the front face comprises at least one window opening into the upper portion of the chamber; anda semi-permeable membrane covering at least a portion of the at least one window opening.2. The cassette according to claim 1 , further comprising a semi-permeable membrane covering at least a portion of the at least one window opening.3. The cassette according to claim 2 , wherein the semi-permeable membrane is removably secured to the front face of the cassette.4. The cassette according to claim 3 , wherein the semi-permeable membrane is configured to allow buffer solution outside the cassette to pass through the membrane into the upper portion of the chamber claim 3 , and to block passage of a biomolecule contained within the upper portion of the ...

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07-11-2013 дата публикации

ELECTROPHORESIS APPARATUS, CAPILLARY ARRAY, AND CAPILLARY UNIT

Номер: US20130292251A1
Принадлежит: HITACHI HIGH-TECHNOLOGIES CORPORATION

In an apparatus for analyzing small number of samples, wasteful consumption of a polymer as a separation Medium is suppressed. 1. A capillary array in a capillary array electrophoresis apparatus having a light emission transmission/detection system of applying an exciting light to a capillary at the end of a capillary array comprising a plurality of capillaries arranged on a plane , the exciting light propagating to adjacent capillaries successively and crossing the capillary array , and focusing light emission generated in the capillary array over a light collection/light detection device , in whichthe capillaries are detachable each independently in a state being sealed with a separation medium to the electrophoresis apparatus,a capillary end on the side of the cathode of the apparatus is connected with an electroconductive passing-through pipe, andboth of the cathode and anode are connected to the apparatus vertically to the surface of a buffer solution.2. The capillary array according to claim 1 , whereinthe capillary is sealed with a separation membrane, capillary ends on the side of the anode and on the side of the cathode are tightly sealed with a plastic film, and in this state, can be stored for a long time and attached/detached to/from the apparatus.3. The capillary array according to claim 1 , whereinthe separation medium is sealed, the capillary end on the side of the anode is tightly closed with an electroconductive resin can be stored for a long time and, attached/detached to/from the apparatus in this state.4. The capillary array according to claim 1 , whereinthe coating is peeled at a detection portion by irradiation of exciting light, and both ends of the capillary are fixed by an identical planar member.5. A capillary array in a capillary array electrophoresis apparatus having a light emission transmission/detection system of applying an exciting light to a capillary at the end of a capillary array comprising a plurality of capillaries arranged on ...

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28-11-2013 дата публикации

Microfluidic Processing of Target Species in Ferrofluids

Номер: US20130313113A1
Автор: Koser Hur
Принадлежит: YALE UNIVERSITY

Disclosed are systems, devices, methods, and other implementations, including a device to detect at least one target species in a sample, with the device including a microfluidic channel configured to receive the sample containing the at least one target species and a biocompatible ferrofluid in which the at least one target species is suspended, a detector to determine the at least one target species in the sample, and at least two of electrodes positioned proximate the microfluidic channel, the at least two electrodes configured to generate controllable magnetic forces in the sample containing the ferrofluid when a controllable at least one electrical current is applied to the at least two electrodes. The generated controllable magnetic forces causes the at least one target species to be directed towards the detector. Also disclosed are devices for separating target species in a ferrofluid, and for focusing target species suspended in a ferrofluid. 1. A device for separating a plurality of target species suspended in a biocompatible ferrofluid , the device comprising:a microfluidic channel including at least one sample inlet and at least one outlet, the microfluidic channel having a channel length extending between the at least one sample inlet and the at least one outlet, the microfluidic channel configured to receive a substantially continuous flow of a sample from the at least one sample inlet, the channel configured to flow the sample along the channel length to the at least one outlet, the sample including a plurality of target species and the biocompatible ferrofluid; anda plurality of electrodes positioned proximate the microfluidic channel, the plurality of electrodes configured to generate a magnetic field pattern along at least a portion of the channel length of the microfluidic channel when a current is applied to the plurality of electrodes, the magnetic field pattern configured to cause at least two of the plurality of target species in the flow of ...

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05-12-2013 дата публикации

LEAKAGE CURRENT SENSE CIRCUIT FOR ERROR DETECTION IN AN IMPROVED CAPILLARY ELECTROPHORESIS-ELECTROSPRAY IONIZATION- MASS SPECTROMETRY SYSTEM

Номер: US20130319862A1
Принадлежит:

Aspects of the present innovations relate to improved systems that may perform capillary electrophoresis (CE) and CE in conjunction with electrospray ionization (ESI) as an input to a mass spectrometry system (MS). Embodiments may use a current sense circuit at a high voltage output from an MS-ESI power supply in conjunction with additional elements to identify fault conditions associated with leakage current, to confirm the continuity of CE connections, and to provide improved system protection. 1. A capillary electrophoresis system for use in conjunction with electrospray ionization mass spectrometry systems comprising:a capillary electrophoresis (CE) high voltage power supply having an output and an input;a mass spectrometry (MS) power supply that connects to a high voltage input of a current sense circuit; anda current sense circuit coupled to a MS power supply terminal, such that the MS power supply terminal is electrically coupled to the input of the CE high voltage power supply via the current sense circuit, a conductive fluid capillary, and a return electrode, and wherein the MS power supply terminal is further electrically coupled to the output of the CE high voltage power supply via the current sense circuit, a separation capillary, and an inlet electrode.2. The system of wherein the current sense circuit comprises a sense resistor and buffer amplifier coupled to an absolute value amplifier.3. The system of wherein the current sense circuit further comprises a DC/DC converter coupled to the absolute value amplifier and powering the buffer and absolute value amplifiers;wherein an isolated side of the current sense circuit is floating.4. The system of further comprising a voltage protection device coupled from a system ground to a floating ground of the current sense circuit.5. The system of wherein the voltage protection device comprises a gas discharge tube.6. The system of further comprising a current sense signal path from the sense resistor to a ...

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05-12-2013 дата публикации

COMPOSITION AND METHOD FOR GEL ELECTROPHORESIS WITH IN-SITU CALIBRATION

Номер: US20130319864A1
Принадлежит:

The invention relates to, among other things, a method for performing electrophoresis with in-situ calibration. The method includes combining a volume of a test sample with a volume or quantity of a calibrating sample to form a final volume, where the volume of the calibrating sample includes a known concentration of a calibrator and the final volume includes a known ratio of test sample to calibrating sample. The method also includes depositing a loading fraction in a receiving well of an electrophoretic gel, in which the loading fraction is a fraction of the final volume, and separating the loading fraction along a common separation lane of the electrophoretic gel such that components of the test sample and the calibrator are separated from one another along the common separation lane. The method also includes detecting the calibrator and separated components of the test sample within the common separation lane and measuring the level of the calibrator and separated components of the test sample based on the detecting, thereby performing electrophoresis with in-situ calibration. 1. A method for performing electrophoresis with in-situ calibration , the method comprising:combining a volume of a test sample with a volume of a calibrating sample to form a final volume, wherein the volume of the calibrating sample comprises a known concentration of a calibrator and the final volume comprises a known volumetric ratio of test sample to calibrating sample;depositing a loading fraction in a receiving well of an electrophoretic gel, wherein the loading fraction is a fraction of said final volume;separating the loading fraction along a common separation lane of the electrophoretic gel such that components of the test sample and the calibrator are separated from one another along the common separation lane;detecting the calibrator and separated components of test sample within the common separation lane; andmeasuring the level of the calibrator and separated components of the ...

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09-01-2014 дата публикации

HYDROLYSIS-RESISTANT POLYACRYLAMIDE GELS

Номер: US20140008226A1
Принадлежит: Bio-Rad Laboratories

Polyacrylamide gels that offer high resolution in protein separations and are more stable relative to hydrolysis than conventional polyacrylamide gels that rely on Tris or Tris-Bis as buffering agents are made by incorporating triethanolamine in place of most or all of the Tris or Tris-Bis. 1. A polyacrylamide gel comprising crosslinked polyacrylamide in a buffer solution comprising triethanolamine , said gel having either no or about 0.3 mM or less of tris(hydroxymethyl)aminomethane and either no or about 0.3 mM or less of bis(2-hydroxyethyl)amino-tris(hydroxymethyl)methane.2. (canceled)3. The polyacrylamide gel of wherein said triethanolamine is at a concentration of from about 50 mM to about 150 mM of said gel.4. The polyacrylamide gel of wherein said gel is devoid of tris(hydroxymethyl)aminomethane or is devoid of bis(2-hydroxyethyl)amino-tris(hydroxymethyl)methane.5. (canceled)6. (canceled)7. (canceled)8. The polyacrylamide gel of wherein said gel further comprises an ampholyte.9. The polyacrylamide gel of wherein said ampholyte is glycine at a concentration of from about 50 mM to about 500 mM.10. The polyacrylamide gel of wherein said gel further comprises a conjugate ampholyte.11. (canceled)12. (canceled)13. The polyacrylamide gel of wherein said conjugate ampholyte is taurine.14. The polyacrylamide gel of wherein said gel further comprises a weak acid at a concentration of from about 10 mM to about 500 mM.15. (canceled)16. A method for separating a mixture of proteins by electrophoresis claim 9 , said method comprising (i) loading a gel with a sample of said mixture wherein said gel comprises crosslinked polyacrylamide in a buffer solution comprising triethanolamine claim 9 , said gel having about 0.3 mM or less of tris(hydroxymethyl)aminomethane and about 0.3 mM or less of g/L bis(2-hydroxyethyl)amino-tris(hydroxymethyl)methane claim 9 , and (ii) imposing a voltage across said gel to cause said proteins to migrate through said gel at differential rates ...

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23-01-2014 дата публикации

System and Process for Making Formic Acid

Номер: US20140021059A1
Принадлежит: Liquid Light, Inc.

Methods and systems for electrochemical production of formic acid are disclosed. A method may include, but is not limited to, steps (A) to (D). Step (A) may introduce water to a first compartment of an electrochemical cell. The first compartment may include an anode. Step (B) may introduce carbon dioxide to a second compartment of the electrochemical cell. The second compartment may include a solution of an electrolyte and a cathode. The cathode is selected from the group consisting of indium, lead, tin, cadmium, and bismuth. The second compartment may include a pH of between approximately 4 and 7. Step (C) may apply an electrical potential between the anode and the cathode in the electrochemical cell sufficient to reduce the carbon dioxide to formic acid. Step (D) may maintain a concentration of formic acid in the second compartment at or below approximately 500 ppm. 110.-. (canceled)11. A system for electrochemical production of at least formic acid , comprising: a cathode; and', 'a catholyte, the pH of the catholyte being maintained from 4.3 to 5.5, the catholyte including formic acid maintained at a concentration of no greater than about 500 ppm., 'an electrochemical cell including12. The system of claim 11 , wherein the electrolyte includes at least one of potassium sulfate claim 11 , potassium chloride claim 11 , sodium chloride claim 11 , sodium sulfate claim 11 , lithium sulfate claim 11 , sodium perchlorate claim 11 , or lithium chloride.13. The system of claim 11 , wherein the second cell compartment includes a heterocyclic aromatic amine selected from the group consisting of 4-hydroxy pyridine claim 11 , adenine claim 11 , a heterocyclic amine containing sulfur claim 11 , a heterocyclic amine containing oxygen claim 11 , an azole claim 11 , benzimidazole claim 11 , a bipyridine claim 11 , furan claim 11 , an imidazole claim 11 , an imidazole related species with at least one five-member ring claim 11 , an indole claim 11 , methylimidazole claim 11 , an ...

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30-01-2014 дата публикации

TARGETED DELIVERY OF REAGENTS TO SPOTS ON A PLANAR SUPPORT THROUGH PATTERNED TRANSFER SHEETS

Номер: US20140027284A1
Принадлежит: Bio-Rad Laboratories, Inc.

Molecular species that are immobilized in discrete locations on a planar support such as protein bands on a gel or a blotting membrane or species applied in dots or spots on a membrane are reacted with binding reagents that are applied through a porous hydrophilic transfer sheet placed over the planar support, the sheet having at least one region that is laterally bordered by a barrier with the binding reagent retained within the bordered region. The bordered region is placed directly over an area on the planar support where the molecular species are expected to reside if they are present on the support. The binding reagent is then delivered into the support to contact the species. Targeted delivery of the binding reagent is thus achieved with improved efficiency. 1. A method for determining whether a molecular species is immobilized on a planar support by contacting an aqueous-based binding reagent with said species , said method comprising:(a) placing said planar support in contact with a porous hydrophilic sheet having a laterally delimited region therein that is bordered by a hydrophobic or impermeable barrier and in which said aqueous-based binding reagent is retained, while aligning said laterally delimited region with a spot on said planar support where said species resides if so immobilized on said planar support;(b) causing transfer of said aqueous-based binding reagent from said laterally delimited region of said porous hydrophilic sheet to said spot; and(c) detecting any occurrence of a binding reaction between said aqueous-based binding reagent and said molecular species as an indication of the presence of said molecular species in said spot.2. The method of wherein the barrier is hydrophobic.3. The method of wherein the hydrophobic barrier is wax or silanized transfer sheet.4. The method of wherein the silanized transfer sheet comprises silanized cellulose.5. The method of wherein step (b) is performed by diffusion.6. The method of wherein step (b) is ...

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06-02-2014 дата публикации

Localized Chemical Microgradients

Номер: US20140034498A1
Принадлежит: ONDA VIA, INC.

A device for creating microgradients in solution is disclosed. The device contains a microfluidic channel with openings at each end and two or more small apertures to a bath. Electrodes are placed in the openings at either end of the channel and an electrical power supply is connected to the electrodes. Several distinct current paths exist from one end of the channel to the other. For example current may flow from one electrode, through a portion of the channel, through an aperture into the bath, back through another aperture into the channel, and along another portion of the channel to the other electrode. Current flows along all possible connected paths when an electric field is applied along the channel and induces fluid flow into and out of the apertures in the channel. Fluid flow through the apertures results in the formation of microgradients in solution near the microfluidic channel device. 1. A method of creating microgradients in solution comprising:providing a microchannel having two or more apertures;filling the microchannel with a solution;providing a bath in contact with the apertures of the microchannel; and,applying an electric field along the microchannel.2. A method for positioning or sorting cells comprising:providing a microchannel having two or more apertures to a bath;applying an electric field along the microchannel;introducing cells in solution into the microchannel; and,moving the solution and the cells by electroosmotic flow until electric current flow along the channel drops essentially to zero.3. A method of delivering reagents to cells comprising:providing a microchannel having two or more apertures to a bath;applying an electric field along the microchannel;introducing reagents into the microchannel; and,positioning cells in the bath near the apertures. This application is a division of U.S. Ser. No. 10/711,327 filed on Sep. 10, 2004.The field of the invention generally relates to microfluidic structures and methods of using them to ...

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06-02-2014 дата публикации

COMPOSITIONS AND METHODS FOR IMPROVING RESOLUTION OF BIOMOLECULES SEPARATED ON POLYACRYLAMIDE GELS

Номер: US20140034500A1
Принадлежит: LIFE TECHNOLOGIES CORPORATION

Gels, such as polyacrylamide gels, are provided that include linear polyacrylamide in the stacking gel. Native gels that include linear polyacrylamide in the stacker can be used to separate biomolecular complexes, such as protein complexes. Gel cassettes in which the gap width between front and back plates does not vary by more than 5% at the upper edge of the cassette are also provided. The gel cassettes can be used for electrophoretic separation of proteins and protein complexes on native gels, such as native gels that include linear polyacrylamide in the stacker. The native gels can have multiple wells for electrophoresing at least one sample and/or at least one molecular weight standard. 141-. (canceled)42. A polyacrylamide gel for the separation of biomolecules , comprising a stacking gel and a separating gel , wherein the stacking gel comprises linear , non-crosslinked polyacrylamide at a concentration of from 0.005% to 1% , and wherein the separating gel does not comprise linear , non-crosslinked polyacrylamide.431. The polyacrylamide gel of claim , wherein the acrylamide and bisacrylamide concentration of the stacking gel is between 2% and 6% , and is optionally between 2.5% and 5%.442. The polyacrylamide gel of claim , wherein the concentration of the linear , non-crosslinked polyacrylamide of the stacking gel is from 0.01% to 0.5% , and optionally from 0.02% to 0.1%.45. The polyacrylamide gel of any of claim 42 , wherein the polyacrylamide gel comprises SDS (sodium dodecyl sulfate).46. The polyacrylamide gel of any of claim 42 , wherein the polyacrylamide gel is a non-denaturing gel claim 42 , wherein optionally the non-denaturing gel is a gradient gel or a Blue Native Gel.47. A cassette for performing native gel electrophoresis claim 42 , wherein the cassette contains a polyacrylamide gel of according to and has a consistent gap width across its upper edge.48. The cassette of claim 47 , wherein the cassette is plastic.49. The cassette of claim 47 , ...

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27-02-2014 дата публикации

TWO-DIMENSIONAL GEL ELECTROPHORESIS APPARATUS AND METHOD

Номер: US20140054172A1
Принадлежит: BIOCULE (SCOTLAND) LIMITED

Two-dimensional gel electrophoresis apparatus includes an electrophoresis zone having four edges defined by a plurality of electrodes. A pair of opposed edges are defined by groups of discrete electrodes. Discrete electrodes within each group are electrically isolated from each other while the other pair of opposed edges are used to generate an electrical field. As a result, the electrical field is less distorted than would be the case if each edge was defined by a single elongate electrode. The apparatus can be provided as a cassette with electrodes configured to guide gas generated during electrolysis out of the cassette through apertures, to reduce the build up of combustible gases. 1. Two-dimensional gel electrophoresis apparatus comprising: a plurality of electrodes defining an electrophoresis zone and a gel support for retaining an electrophoresis gel in the electrophoresis zone , the electrophoresis zone having at least four edges , each of which is defined by at least one of the said plurality of electrodes , the at least four edges comprising first and second pairs of opposed edges , wherein at least one of the second pair of edges is defined by a group of discrete electrodes.2. Two-dimensional gel electrophoresis apparatus according to claim 1 , wherein both of the second pair of edges are defined by respective groups of discrete electrodes.3. Two-dimensional gel electrophoresis apparatus according to claim 1 , wherein one or both of the first pair of edges are defined by respective groups of discrete electrodes.4. Two-dimensional gel electrophoresis apparatus according to claim 1 , wherein each of the plurality of electrodes has a respective electrical contact claim 1 , in direct electrical communication with only one said electrode claim 1 , for connection to isolating means claim 1 , thereby enabling the electrodes within each group to be isolatable from each other or connectable to each other by way of isolating means.5. Two-dimensional gel ...

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06-03-2014 дата публикации

MICROFLUIDICS WITH WIRELESSLY POWERED ELECTRONIC CIRCUITS

Номер: US20140061049A1
Принадлежит:

Techniques, devices and systems are described for incorporating a printed circuit with a microfluidic device and wirelessly powering the microfluidic device. In one aspect, a microfluidic device includes a substrate with a fluidic channel to provide a path for a fluid with particles. The fluidic channel includes fluid inlet and outlet. A pair of electrodes near the inlet and the outlet guides the particles toward a center of the fluidic channel using negative-dielectrophoresis (DEP) effect in response to an alternating current (AC) frequency voltage received at the pairs of electrodes. Additional pairs of electrodes are disposed along a border of the fluidic channel between the pairs of electrodes near the inlet and the outlet of the fluidic channel to isolate a subpopulation of the particles using positive and negative DEP effects in response to AC voltages of different frequencies received at different ones of the additional pairs of electrodes. 1. A microfluidic device , comprising:a substrate comprising a fluidic channel to provide a path for a fluid with suspended particles, wherein the fluidic channel includes an inlet and an outlet for the fluid;a pair of electrodes positioned near the inlet and the outlet of the fluidic channel to guide the particles toward a center of the fluidic channel using negative dielectrophoresis (DEP) effect in response to an alternating current (AC) frequency voltage received at the pair of electrodes; andadditional electrodes positioned along a border of the fluidic channel between the two pairs of electrodes to isolate a subpopulation of the particles using positive and negative DEP effects in response to AC voltages of different frequencies received at different ones of the additional electrodes.2. The microfluidic device of any of , , and , wherein the fluidic channel comprises a permanent fluidic channel formed on the substrate.3. The microfluidic device any of , , and , wherein the fluidic channel comprises a removable ...

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06-03-2014 дата публикации

SYSTEM FOR RAPID HIGH-RESOLUTION GEL ELECTROPHORESIS

Номер: US20140061050A1
Принадлежит: LIFE TECHNOLOGIES CORPORATION

Electrophoretic systems, formulations and methods are described which allow a user to perform electrophoresis experiments under conditions of high voltage and with reduced run time. An electrophoretic system, formulation or method may be run at 50% higher field strength than comparable systems already in use in the art. The presently described systems and formulations may be run at voltages above 225 V, above 250 V, above 275 V, above 300 V, above 325 V or above 350 V. The time required for performing an electrophoresis experiment may be reduced to less than about 30 minutes, less than about 20 minutes, less than about 15 minutes or less than about 12 minutes. 1. A discontinuous buffer electrophoretic system comprising:an electrophoretic separation gel, said electrophoretic separation gel comprising a gel buffer system, said buffer system comprising:a gel amine buffer; anda gel ampholyte; andan aqueous running buffer comprising a buffer ampholyte.2. The discontinuous buffer electrophoretic system according to claim 1 , wherein the separation gel comprises acrylamide claim 1 , agarose claim 1 , or acrylamide and agarose.3. The discontinuous buffer electrophoretic system according to claim 1 , wherein the separation gel comprises acrylamide.4. The discontinuous buffer electrophoretic system according to claim 1 , wherein the separation gel comprises between about 6 to about 25 wt. % acrylamide.5. The discontinuous buffer electrophoretic system according to claim 1 , wherein the separation gel comprises between about 8 to about 20 wt. % acrylamide.6. The discontinuous buffer electrophoretic system according to claim 1 , wherein the separation gel comprises between about 8 to about 15 wt. % acrylamide.7. The discontinuous buffer electrophoretic system according to claim 1 , wherein the electrophoretic separation gel further comprises a stacking gel coupled to one end thereof.8. The discontinuous buffer electrophoretic system according to claim 7 , wherein the stacking ...

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06-03-2014 дата публикации

EXPANDING CAM LOCK FOR SEALING SLAB GELS IN AN ELECTROPHORESIS APPARATUS

Номер: US20140064838A1
Автор: Jackson Thomas R.
Принадлежит: LIFE TECHNOLOGIES CORPORATION

An expanding cam lock for use with an electrophoresis system is disclosed herein. The cam lock allows the simultaneous use of multiple slab gel cassettes in first and second buffer core assemblies in an electrophoresis system while maintaining the necessary compressive force to create a liquid-tight seal between the anode and cathode buffer solutions. In one example embodiment, the expanding cam lock includes a base plate with a first surface adapted to engage the first buffer core assembly and a follower plate having second surface adapted to engage the second buffer core assembly, buffer dam or buffer displacement dam. The base plate and the follower plate are slidably coupled together and are designed for insertion between the first buffer core assembly and the second buffer core assembly, buffer dam or buffer displacement dam in the electrophoresis container. A cam is positioned between and moveably coupled with the base plate and the follower plate. The cam is movable from a first position to a second position to urge the first and second surfaces to secure the gel cassette to the first and second buffer core assemblies. Also provided herein is a buffer displacement dam. Also provided herein are kits and assemblies which incorporate the expanding cam and buffer displacement dam described herein. 1109-. (canceled)110. An expanding cam lock for sealing at least one electrophoresis gel cassette in an electrophoresis apparatus , the expanding cam lock comprising:a base plate having a first surface adapted to engage a first buffer core assembly comprising at least one electrophoresis gel cassette;a follower plate having second surface adapted to engage a second buffer core assembly comprising at least one electrophoresis gel cassette;a cam positioned between and moveably coupled with the base plate and the follower plate;wherein, the base plate and the follower plate are slideably coupled together and configured for insertion between the first buffer core assembly ...

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03-04-2014 дата публикации

High Speed, High Resolution Compositions, Methods and Kits for Capillary Electrophoresis

Номер: US20140090980A1
Принадлежит: APPLIED BIOSYSTEMS, LLC

The invention provides compositions, methods and kits for high speed, high resolution of analytes by capillary electrophoresis starting with uncoated capillaries. The compositions comprise a sieving component, comprising a non-crosslinked acrylamide polymer, and a surface interaction component, comprising at least one uncharged and non-crosslinked water-soluble silica-adsorbing polymer. Methods for employing the novel compositions in capillary electrophoresis are provided. Kits comprising the novel compositions for use in the novel methods are also provided. 1. A composition for separating analytes by capillary electrophoresis comprising:a sieving component comprising a non-crosslinked acrylamide polymer having a molecular weight between about 1,000,000 Daltons (Da) and 3,000,000 Da; and{'sub': 1', '3', '1', '3', '1', '3', '1', '3, 'a surface interaction component comprising one or more non-crosslinked polymers selected from the group consisting of N,N-disubstituted polyacrylamide and N-substituted polyacrylamide, wherein the N-substituents are selected from the group consisting of Cto Calkyl, halo-substituted Cto Calkyl, methoxy-substituted Cto Calkyl, and hydroxyl-substituted Cto Calkyl; wherein the sieving component and the surface interaction component are the same or different;'}wherein the composition does not include a crosslinked polymer gel.2. The composition according to claim 1 , wherein the composition has a viscosity of less than 10 claim 1 ,000 centipoise at 25° C.3. The composition according to claim 1 , wherein the composition has a viscosity of less than 5 claim 1 ,000 centipoise at 25° C.4. The composition according to claim 1 , wherein the composition has a viscosity of less than 1000 centipoise at 25° C.5. The composition according to claim 1 , wherein the composition has a viscosity of less than 600 centipoise at 25° C.6. The composition of claim 1 , wherein the one or more non-crosslinked polymers comprises poly(N claim 1 ,N-dimethylacrylamide ...

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03-04-2014 дата публикации

COMBUSTIBLE FUEL AND APPARATUS AND PROCESS FOR CREATING THE SAME

Номер: US20140090986A1
Автор: Bethurem Gary J.
Принадлежит:

Features for an aqueous reactor include a field generator. The field generator includes a series of parallel conductive plates including a series of intermediate neutral plates. The intermediate neutral plates are arranged in interleaved sets between an anode and a cathode. Other features of the aqueous reactor may include a sealed reaction vessel, fluid circulation manifold, electrical power modulator, vacuum port, and barrier membrane. Methods of using the field generator include immersion in an electrolyte solution and application of an external voltage and vacuum to generate hydrogen and oxygen gases. The reactor and related components can be arranged to produce gaseous fuel or liquid fuel. In one use, a mixture of a carbon based material and a liquid hydrocarbon is added. The preferred carbon based material is powdered coal. 1. An apparatus for generating an electric field , comprising:one or more arrays of plates, the first array including electrically conductive parallel spaced-apart plates supported by a non-electrically conductive framework, wherein the first array of plates includes one or more plates capable of being a cathode at a first end of the array, one or more plates capable of being an anode at a second end of the array opposite to the first end, and one or more neutral plates interposed between the plates capable of being a cathode and an anode, wherein the neutral plates are arranged in interleaved neutral subsets, a first neutral subset including at least three neutral plates electrically connected together and electrically isolated from the plates capable of being a cathode and an anode and any other neutral subsets.2. The apparatus of claim 1 , wherein the plates of each subset is interleaved with the plates of other of the subsets.3. The apparatus of claim 1 , wherein the plates are substantially comprised of a material of high conductivity plated with a catalyst interactive in electrolysis.4. The apparatus of claim 1 , wherein the ...

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01-01-2015 дата публикации

Fluidic Chip with Displacable Patterned Layer for Detecting Fluid Pressure

Номер: US20150000416A1
Принадлежит: AGILENT TECHNOLOGIES, INC.

A fluidic chip device configured for processing a fluid, wherein the fluidic chip device comprises a plurality of layers laminated to one another, wherein at least a part of the layers comprises a patterned section of an alternating sequence of bars and fluidic channels for conducting the fluid under pressure, the patterned section being configured for being displaceable in response to the pressure, and a pressure detector responding to the displacement of the patterned section by generating a detector signal being indicative of a value of the pressure. 1. A fluidic chip device configured for processing a fluid , the fluidic chip device comprising:a plurality of layers laminated to one another;wherein at least a part of the layers comprises a patterned section of an alternating sequence of bars and fluidic channels for conducting the fluid under pressure, the patterned section being configured for being displaceable in response to the pressure; anda pressure detector configured for responding to the displacement of the patterned section by generating a detector signal being indicative of a value of the pressure.2. The fluidic chip device according to claim 1 , comprising:a further patterned section of an alternating sequence of further bars and further fluidic channels for conducting a further fluid under pressure, wherein the further patterned section is configured for being displaceable in response to the pressure of the further fluid;wherein the pressure detector is configured for responding to the displacement of the further patterned section by generating a further detector signal being indicative of a value of the pressure of the further fluid; anda differential pressure determining unit configured for determining information related to a pressure difference between the fluid and the further fluid based on the detector signal and based on the further detector signal.3. The fluidic chip device according to claim 2 , wherein the further patterned section is ...

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01-01-2015 дата публикации

NUCLEIC ACID SAMPLE PREPARATION

Номер: US20150001082A1
Принадлежит:

The present invention includes methods, devices and systems for isolating a nucleic acid from a fluid comprising cells. In various aspects, the methods, devices and systems may allow for a rapid procedure that requires a minimal amount of material and/or results in high purity nucleic acid isolated from complex fluids such as blood or environmental samples. 1. A method for isolating a nucleic acid from a fluid , the method comprising:a. applying the fluid to a device, the device comprising an array of electrodes capable of establishing an AC electrokinetic field region;b. concentrating cells and/or other particulate material in the fluid in a first AC electrokinetic field region, wherein the first AC electrokinetic field region is a dielectrophoretic low field region and the conductivity of the fluid is 300 mS/m or less;c. isolating nucleic acid in a second AC electrokinetic field region, wherein the second AC electrokinetic field is a dielectrophoretic high field region; andd. flushing the concentrated cells and/or other particulate material from the first AC electrokinetic field region.2. The method of claim 1 , wherein the AC electrokinetic field is produced using an alternating current having a voltage of 1 volt to 40 volts peak-peak claim 1 , and/or a frequency of 5 Hz to 5 claim 1 ,000 claim 1 ,000 Hz and duty cycles from 5% to 50%.3. The method of claim 1 , wherein the nucleic acid is deoxyribonucleic acid (DNA) claim 1 , ribonucleic acid (RNA) or a combination thereof.4. The method of claim 1 , wherein the electrodes are selectively energized to provide the first dielectrophoretic field region and subsequently or continuously selectively energized to provide the second dielectrophoretic field region.5. The method of claim 1 , wherein the array of electrodes is spin-coated with a hydrogel having a thickness between about 0.1 microns and 1 micron.6. The method of claim 5 , wherein the hydrogel comprises two or more layers of a synthetic polymer.7. The method ...

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07-01-2016 дата публикации

Polyacrylamide gels for rapid casting, blotting, and imaging, with storage stability

Номер: US20160003772A1
Принадлежит: Bio Rad Laboratories Inc

Hand cast gels, and solutions for their preparation, are described.

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14-01-2016 дата публикации

EXPANDING CAM LOCK FOR SEALING SLAB GELS IN AN ELECTROPHORESIS APPARATUS

Номер: US20160011147A1
Автор: Jackson Thomas
Принадлежит:

An expanding cam lock for use with an electrophoresis system is disclosed herein. In one example embodiment, the expanding cam lock includes a base plate with a first surface adapted to engage the first buffer core assembly and a follower plate having second surface adapted to engage the second buffer core assembly, buffer dam or buffer displacement dam. The base plate and the follower plate are slidably coupled together and are designed for insertion between the first buffer core assembly and the second buffer core assembly, buffer dam or buffer displacement dam in the electrophoresis container. A cam is positioned between and moveably coupled with the base plate and the follower plate. The cam is movable from a first position to a second position to urge the first and second surfaces to secure the gel cassette to the first and second buffer core assemblies. 1109-. (canceled)110. An expanding cam lock for sealing at least one electrophoresis gel cassette in an electrophoresis apparatus , the expanding cam lock comprising:a base plate having a first surface adapted to engage a first buffer core assembly comprising at least one electrophoresis gel cassette;a follower plate having second surface adapted to engage a second buffer core assembly comprising at least one electrophoresis gel cassette;a cam positioned between and moveably coupled with the base plate and the follower plate;wherein, the base plate and the follower plate are slideably coupled together and configured for insertion between the first buffer core assembly and the second buffer assembly in the electrophoresis apparatus, and the cam being movable from a first position to a second position to urge the first surface toward the electrophoresis gel cassette of the first buffer core assembly and the second surface toward the electrophoresis gel cassette of the second buffer core assembly thereby sealing the electrophoresis gel cassettes to the first and second buffer core assemblies within the ...

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09-01-2020 дата публикации

Analysis Method and Analysis System

Номер: US20200011850A1
Автор: Onuma Naotsugu
Принадлежит: ARKRAY, INC.

An analysis method using a microchip which is provided with a capillary flow path, and a sample reservoir connected to the capillary flow path, in which the capillary flow path is filled with a first liquid for electrophoresis, and a second liquid containing a sample is stored in the sample reservoir, and including a pressurization process in which the first liquid is pressurized into the capillary flow path from a side of the capillary flow path that is opposite from the side connected to the sample reservoir, and a separation process in which a voltage is applied between the sample reservoir storing the second liquid and the capillary flow path filled with the first liquid, such that components in the sample contained in the second liquid move in the capillary flow path and the components are separated in the capillary flow path. 1. An analysis method using a microchip that is provided with a capillary flow path and a sample reservoir connected to the capillary flow path , in which the capillary flow path is filled with a first liquid for electrophoresis , and a second liquid containing a sample is stored in the sample reservoir , the analysis method comprising:a pressurization process in which the first liquid is pressurized into the capillary flow path from a side of the capillary flow path that is opposite from a side connected to the sample reservoir; anda separation process in which, after the pressurization process, a voltage is applied between the sample reservoir storing the second liquid and the capillary flow path filled with the first liquid, such that components in the sample contained in the second liquid move in the capillary flow path and the components are separated in the capillary flow path.2. The analysis method according to claim 1 , further comprising claim 1 , before the pressurization process claim 1 , a confirmation process of confirming continuity between the capillary flow path filled with the first liquid and the sample reservoir storing ...

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03-02-2022 дата публикации

Quantum Fluid Operation: Technology for Effective Mixing, Reacting, and Separating Fluids

Номер: US20220032246A1
Автор: Samid Gideon
Принадлежит:

A continuous chemical process is modified to allow parts thereto to be processed one quantum of matter at a time. This offers precision and efficiency beyond what is possible with the continuous mode. This Quantum Fluid Operation (QFO) is applied to basic unit operations: mixing, reacting, separating. 1. A system called “Quantum Fluid Operation” (QFO) for secluding a quantum of fluid in a continuous industrial flow , and treating this quantum as batch operation without affecting the control flow before and after the QFO; the QFO comprising:(i) input fluid capacity tanks, (A tanks),(ii) output fluid capacity tanks, (C tanks),(iii) a quantum fluid container, (B),(iv) operational implements,{'sub': 1', '2', 'f', 'a', 'c', 'a', 'in, 'the total flow of all the fluids a, a, . . . athrough the f A tanks is at a constant flow rate q, and the constant flow rate to the output fluid capacity tank is q=q; a quantum of fluid of measure Q is taken out of the f A tanks during time interval t, and is accumulated in the quantum fluid container, (B), which is big enough to contain the quantum fluid;'}{'sub': 'q', 'next the operational implements operate (treat) on the quantum Q contained in B for a period of time t;'}{'sub': 'out', 'claim-text': {'br': None, 'i': Q=q', 't', '+t', '+t', 'q', 't', '+t', '+t, 'sub': a', 'in', 'q', 'out', 'c', 'in', 'q', 'out, '*()=*()'}, 'next the quantum Q is pumped out of the container B to output fluid capacity tanks, (C), over a period of time t, the identified flow rates and timings comply with the following equation{'sub': 'q', 'and where the operational implements operating on the quantum of fluid Q, change the state of the quantum to a desired state, S=S(Q), and where the quantum treating time, t, is extended as needed to insure that the quantum of fluid, Q, leaves the quantum fluid container, (B) at the desired state, S(Q).'}2. The system in where the desired state of Q claim 1 , S(Q) is expressed as the desired temperature of Q claim 1 , (T) ...

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17-01-2019 дата публикации

CAPACITATIVE ELECTROKINETIC DEWATERING OF SUSPENSIONS

Номер: US20190015785A1
Автор: YAZDANBOD Azaroghly
Принадлежит:

Capacitive electrokinetic densification, decontamination and dewatering of suspensions and soils can be performed while controlling and/or preventing chemical and pH changes in the densified material and extracted water. High electrical capacitance electrodes or Electric Double Layer Capacitor (EDLC) electrodes are used which can operate without redox reactions occurring on their surfaces until their developed voltage reaches the standard electrode potential of the electrode. Water-retaining, flexible covers for the EDLC electrodes have drainage and filtering capabilities and are made of a fabric which allows the passage of ions, water and electricity therethrough and facilitate continuous electrical contact between the EDLC electrode and the surrounding suspension. 1. A capacitive electrokinetic process for densifying solids and recovering water from colloidal suspensions without changes in chemical composition and pH of the densified material and the extracted water , the process comprising the steps of:a) providing an insulated container, the insulated container including at least one outlet drain line for removing water therefrom;b) providing an electric current power supply for generating an electric field through the suspension, wherein the power supply includes a positive pole and a negative pole and is capable of polarity reversal;c) providing at least one pair of high capacitance electric double-layer capacitor (EDLC) electrodes connected to opposite poles of the power supply, wherein each electrode of the at least one pair of EDLC electrodes has a specific capacitance of at least 1.0 farad per gram and is spaced apart from the other electrode within the insulated container;d) providing each EDLC electrode with an electrode cover for placement over the EDLC electrode, wherein each electrode cover is made of a fabric which allows the passage of ions, water and electricity therethrough and facilitates electrical contact between the EDLC electrode and the ...

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16-01-2020 дата публикации

Particle sorting apparatus

Номер: US20200016608A1
Принадлежит: Sharp Corp, University of Tokyo NUC

A particle sorting apparatus a channel that includes a trunk channel formed along a specific upper surface and deflection electrode pairs that sort a non-target minute particle and a target minute particle respectively in a direction substantially perpendicular to the specific upper surface and a direction along the specific upper surface by dielectrophoresis.

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17-01-2019 дата публикации

SPACER FOR SIDE LOADED EWOD DEVICE

Номер: US20190017962A1
Принадлежит:

An EWOD device includes a first and second substrate assemblies, and a spacer that spaces apart the first substrate assembly from the second substrate assembly to define a channel between them. The spacer defines fluid input ports that are in fluid communication with the channel, and the spacer is configured for directing fluid from the fluid input ports into the channel. The spacer has a combed spacer configuration to define the fluid input ports, including alternating teeth that extend into the channel from a base region, and the teeth isolate adjacent fluid input ports from each other. The spacer may contact only a portion of the first and second substrate assemblies to form a spacerless region within the EWOD device, and the spacer includes regions that are in contact with both the first and second substrate assemblies and extend into the channel to define a cell-gap of the channel. 1. An electrowetting on dielectric (EWOD) device comprising:a first substrate assembly and a second substrate assembly, wherein the first and second substrate assemblies have opposing inner surfaces; anda spacer portion that positions the first substrate assembly and the substrate assembly to space apart the first substrate assembly inner surface from the second substrate assembly inner surface to define a channel between the opposing inner surfaces of the first and second substrate assemblies;wherein the spacer portion defines a plurality of fluid input ports that are in fluid communication with the channel, and the spacer portion is configured for directing fluid from the fluid input ports into the channel.2. The EWOD device of claim 1 , wherein the spacer portion has a combed spacer configuration to define the plurality of fluid input ports claim 1 , the combed spacer configuration including alternating teeth that extend into the channel from a base region.3. The EWOD device of claim 2 , wherein externally from the channel the teeth isolate adjacent fluid input ports from each ...

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26-01-2017 дата публикации

SAMPLE SEPARATOR AND SAMPLE SEPARATION/ADSORPTION DEVICE

Номер: US20170023522A1
Принадлежит:

A sample separator () comprising an accommodation part () that accommodates a separation medium for electrophoresis, and a support () formed from a porous material. The accommodation part () includes an internal space () filled with the separation medium, and is provided with a supply port () and discharge port () that are in communication with the internal space (). The support () is provided so as to block the discharge port (). 1. A sample separator , comprising:an accommodation part capable of accommodating a separation medium for electrophoresis; anda support body with a porous material as a formation material thereof,wherein the accommodation part has an internal space capable of being filled with the separation medium, and is provided with a supply port and discharge port that are in communication with the internal space, andwherein the support body is provided to block the discharge port.2. The sample separator according to claim 1 , wherein the support body projects from the discharge port.3. The sample separator according to claim 1 , further comprising a retaining member that retains the support body at the discharge port.4. The sample separator according to claim 1 , further comprising a separation medium filled into the internal space claim 1 , wherein the separation medium is formed integrally with the support body.5. The sample separator according to claim 1 , wherein the separation medium is a polyacrylamide gel.6. A sample separation/adsorption device claim 1 , comprising:a first buffer solution tank capable of storing a first buffer solution;a second buffer solution tank capable of storing a second buffer solution;a first electrode disposed in the first buffer solution tank;a second electrode disposed in the second buffer solution tank;{'claim-ref': {'@idref': 'CLM-00001', 'claim 1'}, 'a sample separator according to that is disposed in the first buffer solution tank; and'}a transcriptional body that transfers a sample,wherein the sample separator ...

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24-04-2014 дата публикации

METHOD AND DEVICE FOR OPTICAL ANALYSIS OF BIOPOLYMER

Номер: US20140110259A1
Принадлежит: HITACHI HIGH-TECHNOLOGIES CORPORATION

A biopolymer optical analysis apparatus is provided with a biopolymer characteristic analysis chip including a solid substrate, nanopores provided in the substrate, and electrically conductive thin films on the substrate, at least portions of the thin films facing the nanopores, a light source and an irradiation optical system for producing Raman scattered lights from biopolymers entering the nanopores, and a detection device including a Raman scattered light collecting system, a separating component that splits collected light into transmitted light and reflected light, an image-forming optical system through which the split lights form images, and a two-dimensional detector for detecting the lights forming the images. External light from the light source and the irradiation optical system is applied to the characteristic analysis chip, and the detection device detects Raman scattered lights from biopolymers in the analysis chip. Characteristics of monomer units constituting the biopolymers are analyzed from the results of detection. 1. A biopolymer optical analysis apparatus comprising:a biopolymer characteristic analysis chip comprising a solid substrate, a plurality of nanopores provided in the solid substrate, and an electrically conductive thin film disposed on the solid substrate, wherein at least a portion of the electrically conductive thin film faces the nanopores;a light source and an irradiation optical mechanism for producing Raman scattered lights from a biopolymer entering the nanopores;a light collecting mechanism for Raman scattered light;a separating component that splits collected light into transmitted light and reflected light at different ratios for each components of the biopolymer;an image-forming optical mechanism through which the split lights form images on a detector; anda two-dimensional detector for detecting the lights forming the images,wherein external light is applied from the light source and the irradiation optical system to the ...

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24-04-2014 дата публикации

CHIP FOR ELECTROPHORESIS AND METHOD FOR PRODUCING SAME

Номер: US20140110261A1
Принадлежит: SHARP KABUSHIKI KAISHA

A chip () for electrophoresis is provided, the chip including a gel () composed of a polymer prepared by polymerizing a monomer, and a support () configured to support the gel (). In the chip () for electrophoresis, a surface of the support () in contact with the gel () is covered with a surface treatment compound () containing the monomer or a derivative of the monomer. 1. A chip for electrophoresis , comprising:a gel composed of a polymer prepared by polymerizing a monomer; and a support configured to support the gel,wherein a surface of the support in contact with the gel is covered with a surface treatment compound containing the monomer or a derivative of the monomer,the gel is an agarose gel, andthe surface treatment compound contains a monosaccharide.24.-. (canceled)5. The chip for electrophoresis according to claim 1 , wherein the monosaccharide is a monosaccharide selected from the group consisting of galactose and anhydrogalactose.6. The chip for electrophoresis according to claim 1 , wherein the surface treatment compound is covalently bonded to the gel.7. The chip for electrophoresis according to claim 1 , wherein the gel is formed so as to protrude from the support.8. A method for producing a chip for electrophoresis claim 1 , the chip including a gel composed of a polymer prepared by polymerizing a monomer claim 1 , and a support configured to support the gel claim 1 , the method comprising:a covering step of covering at least part of a surface of the support with a surface treatment compound containing the monomer or a derivative of the monomer; anda gel formation step of forming the gel on a covered region of the surface of the support,wherein in the gel formation step, a gel formation solution to form the gel is ejected onto the region with an ink jet nozzle.910.-. (canceled)118. The method for producing a chip for electrophoresis according to claim 1 , wherein in the covering step claim 1 , the surface treatment compound is subjected to graft ...

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29-01-2015 дата публикации

METHODS FOR ELECTROELUTION OF BIOMOLECULES

Номер: US20150027891A1
Принадлежит: GENERAL ELECTRIC COMPANY

A method of eluting biomolecules, such as nucleic acids from a biological sample by electroelution is provided. An example of a method includes various steps, such as loading the biological sample to a device comprising a housing, at least two conductive redox polymer electrodes operationally coupled to the housing and a biomolecule impermeable layer disposed on at least one of the electrodes. The loading of sample is followed by initiating an electrical connection to generate an electric field strength sufficient to elute biomolecules from the biological sample; and eluting the biomolecules from the biological sample. 1. A method of eluting biomolecules from a biological sample , comprising:loading the biological sample to a device comprising a housing, at least two conductive redox polymer electrodes operationally coupled to the housing and a biomolecule impermeable layer disposed on at least one of the electrodes;initiating an electrical connection to generate an electric field strength sufficient to elute biomolecules from the biological sample; andeluting the biomolecules from the biological sample.2. The method of claim 1 , wherein the loading of the biological sample is effected by direct loading of the biological sample to the housing claim 1 , loading of a biological sample laden substrate into the housing claim 1 , loading of the biological sample in a package configured to release the sample during operation claim 1 , or combinations thereof.3. The method of claim 1 , further comprising wetting the electrodes and biomolecule impermeable layer.4. The method of claim 3 , further comprising electrophoretically eluting the biomolecules from the biological sample.5. The method of claim 1 , further comprising electrophoretically driving the eluted biomolecules to a surface of the biomolecule impermeable layer.6. The method of claim 1 , further comprising eluting out the impurities from the housing.7. The method of claim 1 , further comprising releasing the ...

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29-01-2015 дата публикации

DEVICES AND SYSTEMS FOR ELUTION OF BIOMOLECULES

Номер: US20150027894A1
Принадлежит: General Electric CompanySchenectady

A device and a system for eluting biomolecules from biological sample by electroelution are provided. The device for electroelution of biomolecules from a biological sample is constituted with a housing configured to receive an electrolyte and the biological sample, at least two electrodes comprising conductive redox polymers operationally coupled to the housing, and a biomolecule impermeable layer disposed on at least one of the electrodes. The biomolecule impermeable layer disposed on at least one of the electrodes to prevent the biomolecules from reaching the electrode. A system is provided, wherein the system comprises a sample collection port, one or more reservoirs comprising a buffer, a solvent, a reagent or combinations thereof, an device for electroelution, and a controller. 1. A device , comprising:a housing configured to receive an electrolyte and a biological sample comprising biomolecules;at least two electrodes comprising conductive redox polymers operationally coupled to the housing, anda biomolecule impermeable layer disposed on one of the electrodes to prevent contact between the conductive redox polymers and the biomolecules.2. The device of claim 1 , wherein the conductive redox polymers comprise a poly(3 claim 1 ,4-ethylenedioxythiophene) poly(styrenesulfonate) (Pedot: PSS) material.3. The device of claim 1 , wherein the conductive redox polymers are treated to reduce leaching of one or more components of the electrode materials from the electrodes.4. The device of claim 3 , wherein the treated conductive redox polymers comprise cross-linked two or more of the polymers claim 3 , polymers and dopant or combinations thereof.5. The device of claim 3 , wherein the treated conductive redox polymers comprise cross-linked redox polymers and dopant.6. The device of claim 1 , wherein 0.05 μg to 0.05 mg per mmof the conductive redox polymers are in contact with the housing.7. The device of claim 1 , wherein the electrodes are configured to generate an ...

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25-01-2018 дата публикации

RADIOISOTOPE RECOVERY

Номер: US20180025801A1
Принадлежит:

The present invention relates to a method and an apparatus for separating and recovering a radioisotope from a solution. More particularly, certain embodiments of the invention relate to a method for recovering a radioisotope from a solution by electro-trapping and release using a microfluidic cell (). The radioisotope may subsequently be used in the preparation of radiopharmaceuticals. 1. A method for separating and recovering a radioisotope from an aqueous solution comprising the radioisotope , the method comprising:using a microfluidic device comprising a chamber;flowing the aqueous solution to the chamber, the chamber comprising a first electrode and a second electrode;generating a first electric field between the first and second electrodes, thereby trapping the radioisotope on the first electrode;flowing an organic-based solution to the chamber comprising the first and the second electrodes; andgenerating a second electric field between the first and the second electrodes;wherein the second electric field has an opposing polarity to the first electric field, thereby releasing the radioactive isotope from the first electrode into the organic-based solution; andwherein the first electrode is formed from a carbon rod or section thereof.2. The method of claim 1 , further comprising one or more of the features selected from:flowing the aqueous solution at a flow rate of at least 0.1 mL/min;flowing the organic-based solution at a flow rate of at least 0.05 mL/min;applying a voltage of no greater than 30 V across the first and second electrodes to generate the first electric field; andapplying a voltage of no greater than 10 V across the first and second electrodes to generate the second electric field.35-. (canceled)6. The method of claim 1 , wherein the chamber has a volume of no greater than approximately 50 μL.7. The method of claim 1 , wherein the first electrode has a flat surface comprising a plurality of recesses and/or the first electrode has a polished ...

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28-01-2021 дата публикации

METHOD OF PURITY DETERMINATION BY CAPILLARY ELECTROPHORESIS

Номер: US20210025847A1
Автор: BECKMAN JEFF W., QUAN Guan
Принадлежит: Bristol-Mayers Squibb Company

Protein separation by capillary electrophoresis using a buffer composition comprising hydrophobic detergents that have alkyl chains longer than 12 carbon atoms. The formation of high molecular weight artifacts is suppressed. 1. (canceled)2. A method for improving capillary electrophoresis protein peak separation efficiency (PSE) , comprising separating a denatured protein of interest in the sample in a hydrophobic detergent gel buffer.3. A method for improving protein purity determination by capillary electrophoresis , comprising separating a denatured protein of interest in the sample in a hydrophobic detergent gel buffer.4. (canceled)5. The method of claim 3 , wherein the hydrophobic detergent comprises the same charged sulfate head group and sodium counter-ion with alkyl chain lengths selected from 14 and 16.67-. (canceled)8. An electrophoresis buffer composition comprising a hydrophobic detergent comprising a sulfate head group and a hydrophobic tail comprising an alkyl chain having greater than 12 carbon atoms.9. The buffer composition of claim 8 , wherein the alkyl chain has carbon atoms less than 19 or 20.10. The buffer composition of claim 9 , wherein the alkyl chain has carbon atoms of 13 claim 9 , 14 claim 9 , 15 claim 9 , 16 claim 9 , 17 claim 9 , or 18.11. The buffer composition of claim 8 , wherein the hydrophobic detergent is more hydrophobic than sodium dodecyl sulfate.12. The buffer composition of claim 8 , wherein the hydrophobic detergent is capable of inducing improved protein peak separation efficiency by capillary sieving electrophoresis compared to sodium dodecyl sulfate.13. The buffer composition of claim 8 , wherein the hydrophobic detergent is selected from a group consisting of sodium tridecyl sulfate claim 8 , sodium tetradecyl sulfate (STS) claim 8 , sodium pentadecyl sulfate claim 8 , sodium hexadecyl sulfate (SHS) claim 8 , sodium heptadecyl sulfate claim 8 , and sodium octadecyl sulfate (SOS).14. The buffer composition of claim 13 , ...

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04-02-2016 дата публикации

SINGLE MOLECULE FILTER AND SINGLE MOLECULE ELECTROGRAPH, AND PROCESS FOR MAKING AND USING SAME

Номер: US20160033451A1
Принадлежит:

A single molecule filter includes: a membrane including: a first surface; a second surface; and a membrane aperture disposed in the membrane and traversing the membrane from the first surface to the second surface, the membrane aperture provided to communicate a single molecule across the membrane; and a nanotube disposed on the membrane and including: a first end disposed on the first surface of the membrane; a second end disposed distal to the first surface; and a tubular aperture extending along the nanotube from the first end to the second end, the tubular aperture provided to communicate the single molecule from the second end of the nanotube to the membrane aperture. 1. A single molecule filter comprising: a first surface;', 'a second surface; and', 'a membrane aperture disposed in the membrane and traversing the membrane from the first surface to the second surface, the membrane aperture provided to communicate a single molecule across the membrane; and, 'a membrane comprising a first end disposed on the first surface of the membrane;', 'a second end disposed distal to the first surface; and', 'a tubular aperture extending along the nanotube from the first end to the second end, the tubular aperture provided to communicate the single molecule from the second end of the nanotube to the membrane aperture., 'a nanotube disposed on the membrane and comprising2. The single molecule filter of claim 1 , further comprising an interfacial layer disposed on the first surface and interposed between the first end of the nanotube and the first surface of the membrane.3. The single molecule filter of claim 1 , wherein the membrane comprises a plurality of layers.4. The single molecule filter of claim 3 , wherein the plurality of layers comprises: a first semiconductor; and', 'the first surface; and, 'a first layer comprising a second semiconductor; and', 'the second surface., 'a second layer opposing the first layer and comprising5. The single molecule filter of claim 4 , ...

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30-01-2020 дата публикации

TASK SPECIFIC CHELATING IONIC LIQUIDS FOR REMOVAL OF METAL IONS FROM AQUEOUS SOLUTION VIA LIQUID/LIQUID EXTRACTION AND ELECTROCHEMISTRY

Номер: US20200030716A1
Принадлежит:

Disclosed are methods of extracting metal ions using ionic liquids (ILs), IL complexes, and mixtures comprising an IL and a metal-chelating group. Also disclosed are IL complexes, and mixtures comprising an IL and a metal-chelating group. 1. A method of removing metal cations from an ionic liquid mixture , comprising:providing an ionic liquid mixture comprising an ionic liquid (IL), wherein the IL comprises a metal-chelating group, and a plurality of metal cations; andapplying an electrical potential to the ionic liquid mixture, thereby removing from the ionic liquid mixture the plurality of metal cations.24.-. (canceled)5. The method of claim 1 , wherein applying the electrical potential causes the plurality of metal cations to be electrochemically reduced.6. The method of claim 1 , wherein applying the electrical potential causes the plurality of metal cations to be electrochemically reduced to metal atoms.7. The method of claim 1 , wherein the metal cation has a charge of +1 claim 1 , +2 claim 1 , or +3.8. The method of claim 7 , wherein the metal cation is a cation of Ag claim 7 , Ca claim 7 , Cd claim 7 , Co claim 7 , Cr claim 7 , Cu claim 7 , Er claim 7 , Fe claim 7 , Hg claim 7 , Mg claim 7 , Mn claim 7 , Nb claim 7 , Ni claim 7 , Pb claim 7 , Pd claim 7 , Sc claim 7 , Sn claim 7 , Sr claim 7 , V claim 7 , Zn claim 7 , Ce claim 7 , Dy claim 7 , Er claim 7 , Eu claim 7 , Fe claim 7 , Gd claim 7 , Ho claim 7 , La claim 7 , Lu claim 7 , Nb claim 7 , Nd claim 7 , Pm claim 7 , Pr claim 7 , Sm claim 7 , Tb claim 7 , Tm claim 7 , Yb claim 7 , or a mixture thereof.912.-. (canceled)13. The method of claim 1 , wherein the metal-chelating group is selected from the group consisting of an ethylaminediacetic acid moiety claim 1 , a crown ether claim 1 , a dithizone claim 1 , a hydroxyquinoline claim 1 , 2-thenoyltrifluoroacetone claim 1 , a thiosalicylate claim 1 , a salicylate claim 1 , a thiocarbamate claim 1 , a dithiocarbamate claim 1 , an alkanolamine claim 1 , a ...

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17-02-2022 дата публикации

GEL, MARKER, AND KIT FOR PROTEIN ELECTROPHORESIS, AND APPLICATION OF GEL

Номер: US20220050078A1
Принадлежит:

A gel for protein electrophoresis includes a separating gel and a stacking gel disposed on the separating gel. The separating gel is a polyacrylamide gel including a surfactant, and is alkaline. The surfactant of the separating gel includes 0.025-0.1% (m/v) of sodium lauroyl sarcosinate. The ratio of the molar concentration of the surfactant to the mass concentration of a loading protein is between 0.04 mmol/g and 11.56 mmol/g. 1. A gel for protein electrophoresis , comprising separating gel and a stacking gel disposed on the separating gel; wherein the separating gel is a polyacrylamide gel comprising a surfactant , and is alkaline; the surfactant of the separating gel comprises 0.025-0.1% (m/v) of sodium lauroyl sarcosinate; a ratio of a molar concentration of the surfactant to a mass concentration of a loading protein is between 0.04 mmol/g and 11.56 mmol/g.2. The gel of claim 1 , wherein the ratio of the molar concentration of the surfactant to the mass concentration of the loading protein is between 1.16 mmol/g and 9.90 mmol/g.3. The gel of claim 1 , wherein the stacking gel comprises polyacrylamide with a mass volume fraction of 3.5-5% claim 1 , and the surfactant of the stacking gel comprises 0.025-0.1% (m/v) of sodium lauroyl sarcosinate.4. A buffer for protein electrophoresis claim 1 , the buffer comprising a surfactant; the surfactant comprising 0.025-0.1% (m/v) of sodium lauroyl sarcosinate claim 1 , and a ratio of a molar concentration of the surfactant to a mass concentration of a loading protein is between 0.04 mmol/g and 11.56 mmol/g.5. The buffer of claim 4 , being a loading buffer claim 4 , a running buffer claim 4 , or a buffer for dissolving a protein Marker.6. The buffer of claim 5 , wherein the buffer is the loading buffer claim 5 , and a mass concentration of the loading protein is not more than 40.32 mg/mL.7. The buffer of claim 6 , wherein a mass concentration of the loading protein for the buffer for protein electrophoresis is 0.59-2.94 mg/ ...

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30-01-2020 дата публикации

SINGLE-WALLED CARBON NANOTUBE SEPARATION APPARATUS AND SINGLE-WALLED CARBON NANOTUBE SEPARATION METHOD

Номер: US20200031672A1
Автор: NIHEY Fumiyuki
Принадлежит: NEC Corporation

A single-walled carbon nanotube separation apparatus includes: a separation tank accommodating a single-walled carbon nanotube dispersion liquid containing: metallic single-walled carbon nanotubes; and semiconducting single-walled carbon nanotubes; a first electrode and a second electrode that are installed in the separation tank; and a partition wall installed between the first electrode and the second electrode in the separation tank and below the separation tank in a height direction thereof. 1. A single-walled carbon nanotube separation apparatus comprising:a separation tank accommodating a single-walled carbon nanotube dispersion liquid containing: metallic single-walled carbon nanotubes; and semiconducting single-walled carbon nanotubes;a first electrode and a second electrode that are installed in the separation tank; anda partition wall installed between the first electrode and the second electrode in the separation tank and below the separation tank in a height direction thereof.2. The single-walled carbon nanotube separation apparatus according to claim 1 , wherein the separation tank has a double pipe structure including: a first pipe; and a second pipe that is provided to surround an outer circumference of the first pipe claim 1 ,the first pipe and the second pipe communicate with the separation tank on a bottom surface side,the first electrode is installed in the first pipe, andthe second electrode is installed in a gap between the first pipe and the second pipe.3. The single-walled carbon nanotube separation apparatus according to claim 2 , wherein the partition wall is installed at one end portion of the first pipe on the bottom surface side of the separation tank.4. The single-walled carbon nanotube separation apparatus according to claim 3 , wherein an injection port for the single-walled carbon nanotube dispersion liquid is installed inside the partition wall.5. A single-walled carbon nanotube separation method using the single-walled carbon ...

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04-02-2021 дата публикации

SELECTIVE MODIFICATION OF POLYMER SUBUNITS TO IMPROVE NANOPORE-BASED ANALYSIS

Номер: US20210032695A1
Принадлежит:

The present disclosure provides method and systems for improving nanopore-based analyses of polymers. The disclosure provides methods for selectively modifying one or more monomeric subunit(s) of a kind a pre-analyte polymer that results polymer analyte with a modified subunit. The polymer analyte produces a detectable signal in a nanopore-based system. The detectable signal, and/or its deviation from a reference signal, indicates the location of the modified subunit in the polymer analyte and, thus, permits the identification of the subunit at that location in the original pre-analyte polymer. 1(a) translocating a polymer analyte comprising a modified subunit from a first conductive liquid medium to a second conductive liquid medium through a nanopore, wherein the nanopore provides liquid communication between the first conductive liquid medium and the second conductive liquid medium;(b) measuring an ion current between the first conductive liquid medium and the second conductive liquid medium as the polymer analyte passes through the nanopore; and(c) detecting the modified subunit based on the measured ion current.. A method for analyzing a polymer analyte, comprising: This application is a continuation of U.S. application Ser. No. 16/029,396, filed Jul. 6, 2018, which is a continuation of U.S. patent application Ser. No. 14/915,611, filed Feb. 29, 2016, and issued as U.S. Pat. No. 10,017,814 on Jul. 10, 2018, which is the National Stage of International Patent Application No. PCT/US2014/053754, filed Sep. 2, 2014, which claims the benefit of U.S. Patent Application No. 61/872,406, filed Aug. 30, 2013. All of the aforementioned applications are expressly incorporated by reference herein.This invention was made with government support under grant no. R01HG005115, awarded by the National Institutes of Health. The government has certain rights in the invention.The sequence listing associated with this application is provided in text format in lieu of a paper copy and ...

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12-02-2015 дата публикации

POLYACRYLAMIDE GEL FOR USE WITH TRADITIONAL AND NON-TRADITIONAL ELECTROPHORESIS RUNNING BUFFERS

Номер: US20150041321A1
Принадлежит:

Disclosed are gel systems prepared with a substantially neutral gel buffer solution, which contains an amine base and at least one zwitterionic component and an acid component. Methods of making and using these gel systems are also disclosed herein. 1. A method of preparing a gel system , the method comprising: polymerizing acrylamide in the presence of a cross-linking agent and a gel buffer solution , wherein the gel buffer solution comprises an amine base at a concentration of from about 0.005 to about 0.25 M and a zwitterionic component at a concentration of from about 0.005 to about 0.5 M titrated with an acid component that comprises a polyprotic acid to a pH of from about 6.0 to about 9.5.2. The method of claim 1 , wherein the gel buffer solution has a pH of from about 7.5 to about 8.5.3. The method of claim 1 , wherein the gel buffer solution has a pH of from about 6.5 to about 7.5.4. The method of claim 1 , wherein the gel buffer solution comprises the amine base at a concentration of from about 0.025 to about 0.15 M and the zwitterionic component at a concentration of from about 0.05 to about 0.4 M titrated with the acid component to a pH of from about 6.5 to about 7.5.5. The method of claim 1 , wherein the amine base is a primary claim 1 , secondary or tertiary amine.6. The method of claim 1 , wherein the amine base is a primary claim 1 , secondary or tertiary amine with a hydroxylated side chain.7. The method of claim 1 , wherein the amine base is an alcoholamine with branched alkyl chains.8. The method of claim 1 , wherein the amine base is Tris(hydroxymethyl)aminomethane claim 1 , ethanolamine claim 1 , diethanolamine claim 1 , isopropanolamine claim 1 , diisopropanolamine claim 1 , or triisopropanolamine.9. The method of claim 1 , wherein the amine base has a pKof from about 7.5 to about 10.0.10. The method of claim 1 , wherein the zwitterionic component is glycine.11. The method of claim 1 , wherein the zwitterionic component is one or more of glycine ...

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12-02-2015 дата публикации

PROTEIN DETECTION USING MODIFIED CYCLODEXTRINS

Номер: US20150044705A1
Принадлежит:

A method is provided for detecting a protein using a cyclodextrin covalently linked to at least one label. The cyclodextrin can associate with the protein by sequestering an aromatic amino acid side-chain of the protein in its hydrophobic cavity. After contacting the protein with the cyclodextrin, the label can be detected directly or can undergo a chemical interaction with a reagent to form a detectable product. The label can include an indole moiety, which can react with a halo-substituted organic compound upon exposure to UV light and thereby be rendered fluorescent. Alternatively, the label can include a biotin moiety, which can bind to a binding partner such as avidin, or variants thereof, to form a detectable molecular complex. A labeled cyclodextrin can be used in the present methods to detect a protein of interest in an electrophoresis gel or on a blotting membrane. Aromatic amino acid residues of the protein, in particular tryptophan, remain protected from chemical modification due to sequestration by the cyclodextrin, making these methods compatible with downstream applications that require intact protein. Also provided herein are compositions, kits, and electrophoresis gels for use in detecting proteins. 1. A method of detecting a protein , the method comprising:contacting the protein with a cyclodextrin covalently linked to at least one label;contacting the protein with a reagent;causing the reagent to undergo a chemical interaction with the at least one label to form a product;detecting the product, thereby detecting the protein.2. The method of claim 1 , wherein the cyclodextrin is selected from the group consisting of α-cyclodextrin claim 1 , β-cyclodextrin claim 1 , methyl-β-cyclodextrin claim 1 , and γ-cyclodextrin.3. The method of claim 1 , wherein the at least one label comprises an indole moiety.4. The method of claim 3 , wherein the at least one label comprises a tryptophan residue.5. The method of claim 3 , wherein the at least one label does ...

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07-02-2019 дата публикации

Capillary Electrophoresis Apparatus

Номер: US20190041359A1
Принадлежит:

A capillary electrophoresis apparatus is disclosed that does not discharge and achieves both compactness and performance even with a part configuration having insufficient spatial distance or creeping distance. This capillary electrophoresis apparatus is provided with a resistance heater for heating capillaries, an electrode holder that holds capillary electrodes and is connected to a high-voltage unit, and a conductive member that at least partially comprises metal and has been grounded to a low potential. The electrode holder and conductive member are in contact with heat-dissipating rubber disposed there between that composes a structure comprising an insulation member. As a result of this configuration, discharge risk is reduced through the reduction of the potential of parts near the high-voltage unit and the slow reduction of the high potential of the high-voltage unit. 1. A capillary electrophoresis apparatus for analyzing a sample by electrophoresis using a capillary , the apparatus comprising:a heater that heats the capillary;an electrode holder that holds capillary electrodes of the capillary and is connected to a high-voltage unit; anda conductive member that is at least partially formed of metal and is grounded to a low potential, whereinthe electrode holder and the conductive member are in contact with a structure disposed therebetween, andthe structure is an insulation member.2. The capillary electrophoresis apparatus according to claim 1 , whereinthe conductive member is subjected to insulation treatment.3. The capillary electrophoresis apparatus according to claim 2 , whereinthe insulation treatment of the conductive member is performed stepwise according to a distance from the electrode holder.4. The capillary electrophoresis apparatus according to claim 1 , whereinthe capillary and the conductive member are in continuous contact with the structure disposed therebetween.5. The capillary electrophoresis apparatus according to claim 1 , whereinthe ...

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18-02-2016 дата публикации

NUCLEIC ACID SAMPLE PREPARATION

Номер: US20160046926A1
Принадлежит:

The present invention includes methods, devices and systems for isolating a nucleic acid from a fluid comprising cells. In various aspects, the methods, devices and systems may allow for a rapid procedure that requires a minimal amount of material and/or results in high purity nucleic acid isolated from complex fluids such as blood or environmental samples. 2. The method of claim 1 , wherein the first AC electrokinetic region is a dielectrophoretic field region claim 1 , wherein the second AC electrokinetic field region is a dielectrophoretic field region claim 1 , or a combination thereof.3. The method of or claim 1 , wherein the first AC electrokinetic field region is a first dielectrophoretic low field region and the second AC electrokinetic field region is a second dielectrophoretic high field region claim 1 , wherein the conductivity of the fluid is greater than 300 mS/m.4. The method of or claim 1 , wherein the first AC electrokinetic field region is a first dielectrophoretic high field region and the second AC electrokinetic field region is a second dielectrophoretic high field region claim 1 , wherein the conductivity of the fluid is less than 300 mS/m.5. The method of any of the preceding claims wherein the nucleic acid is concentrated in the second AC electrokinetic field region.6. The method of any of the preceding claims claim 1 , wherein the method further comprises flushing residual material from the array and the isolated nucleic acid.7. The method of any of the preceding claims claim 1 , wherein the method further comprises degradation of a residual protein.8. The method of claim 7 , wherein the method further comprises flushing degraded proteins from the array and the isolated nucleic acid.9. The method of any of the preceding claims claim 7 , wherein the method further comprises collecting the nucleic acid.10. The method of any of the preceding claims claim 7 , wherein the first AC electrokinetic field region is produced by an alternating current. ...

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15-02-2018 дата публикации

ELECTROPHORESIS RUNNING TANK ASSEMBLY

Номер: US20180045677A1
Принадлежит:

An electrophoresis running tank assembly uses two opposed rows of LEDs to illuminate DNA-containing gel on a transparent tray positioned between the rows. A respective cylindrical lens is positioned horizontally between each row and a respective edge of the tray. The optical axis of the illumination light is midway between a bottom surface of the gel tray and a top surface of the gel. 1. Assembly for electrophoresis , comprising:at least one tank formed with a gel tray platform including a top surface configured for holding at least one gel tray containing gel;at least an anode reservoir on a first side of the gel tray platform and at least a cathode reservoir on a second side of the gel tray platform, both reservoirs configured for holding buffer during electrophoresis;at least one anode immersed in the anode reservoir and at least one cathode immersed in the cathode reservoir;a first lens engaged with an opening of a housing supporting the tank, the first lens being elongated in a horizontal dimension, the first lens substantially filling the opening, plural first light emitting diodes (LED) arranged in a horizontal row along a first printed circuit board (PCB) comprising at least one connection to a power source to energize the first LEDs, the first PCB being mounted outboard of the first lens, the first LEDs being recessed radially outward of the inner surface and the first lens being located between the first PCB and a first side of the tank.2. The assembly of claim 1 , wherein the first lens is formed on a parallelepiped-shaped transparent block.3. The assembly of claim 2 , comprising a lens seal be disposed between an outside surface of a side wall and the block.4. The assembly of claim 1 , wherein the PCB comprises a skirt to spread and dissipate heat generated when the first LEDs are energized.5. The assembly of claim 1 , comprising a second lens engaged with an opening of a housing supporting the tank claim 1 , the second lens being elongated in a ...

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13-02-2020 дата публикации

NANOCARBON SEPARATION METHOD

Номер: US20200047122A1
Принадлежит: NEC Corporation

A nanocarbon separation method includes: a step of preparing a plurality of liquids with different specific gravities in which at least one of the plurality of liquids is a dispersion liquid in which a mixture of nanocarbons with different properties is dispersed; a step of sequentially injecting the plurality of liquids into an electrophoresis tank so that the specific gravities of the liquids decrease from a bottom to a top of the liquids in a direction of gravitational force; and a step of separating the mixture of the nanocarbons by moving a part of the mixture toward an electrode side disposed in an upper part of the electrophoresis tank and moving a remainder of the mixture toward an electrode side disposed in a lower part of the electrophoresis tank by applying a direct current voltage to the electrodes. 1. A nanocarbon separation method comprising:a step of preparing a plurality of liquids with different specific gravities in which at least one of the plurality of liquids is a dispersion liquid in which a mixture of nanocarbons with different properties is dispersed;a step of sequentially injecting the plurality of liquids into an electrophoresis tank so that the specific gravities of the liquids decrease from a bottom to a top of the liquids in a direction of gravitational force; anda step of separating the mixture of the nanocarbons by moving a part of the mixture of the nanocarbons toward an electrode side disposed in an upper part of the electrophoresis tank and moving a remainder of the mixture of the nanocarbons toward an electrode side disposed in a lower part of the electrophoresis tank by applying a direct current voltage to the electrodes disposed in the upper part and the lower part.2. The nanocarbon separation method according to claim 1 , wherein claim 1 , in the step of preparing the plurality of liquids claim 1 , a dispersion liquid in which a mixture of nanocarbons with two or more kinds of different specific gravities is dispersed claim 1 , ...

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13-02-2020 дата публикации

INJECTING A LIQUID BORNE SAMPLE INTO A FIELD FLOW FRACTIONATOR

Номер: US20200049671A1
Автор: Trainoff Steven P.
Принадлежит: WYATT TECHNOLOGY CORPORATION

The present invention an apparatus and method of injecting a liquid borne sample into a field flow fractionator and a method of forming a top plate and spacer. In an embodiment, the field flow fractionation unit includes a top plate including a sample injection inlet port, a sample injection outlet port, and a spacer including a separation channel cavity defining at least a portion of the separation channel, a sample injection inlet cavity configured to be in fluid contact with the separation channel and located substantially beneath the sample injection inlet port, a sample injection outlet cavity configured to be in fluid contact with the separation channel and located substantially beneath the sample injection outlet port, such that the injection inlet and outlet paths are configured to define an injection channel that is essentially perpendicular to the length of the separation channel spanning the width of the separation channel cavity. 1. A field flow fractionation unit comprising: a sample injection inlet port configured to allow a liquid borne sample to be injected into a separation channel,', 'a sample injection outlet port configured to allow liquid contained within the separation channel to be withdrawn; and, 'a top plate comprising'} [ 'wherein the separation channel is defined by a surface of the top plate, sidewalls of the spacer, and a surface of a membrane,', 'a separation channel cavity defining at least a portion of the separation channel,'}, 'wherein the sample injection inlet cavity is configured to act as an injection inlet path,', 'a sample injection inlet cavity configured to be in fluid contact with the separation channel and located substantially beneath the sample injection inlet port,'}, 'wherein the sample injection outlet cavity is configured to act as an injection outlet path,', 'a sample injection outlet cavity configured to be in fluid contact with the separation channel and located substantially beneath the sample injection outlet ...

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15-05-2014 дата публикации

CAPACITIVE ELECTROKINETIC DEWATERING OF SUSPENSIONS AND SOILS

Номер: US20140131206A1
Автор: YAZDANBOD Azaroghly
Принадлежит:

An apparatus and process for electrophoretic and electro-osmotic densification, decontamination and dewatering of suspensions includes the use of high electrical capacitance electrodes for capacitive generation of electric fields. Polarity reversals between the capacitor electrodes prevent faradic electrolysis and corrosion reactions at the electrodes, and water wettable, flexible linings for the electrodes, having drainage and filtering capabilities, can also be used as interceptor drains positioned adjacent and in full electric contact with the electrodes, as well as within the suspension as flow path dividers. 1. A method for decontamination and densification of and fluid extraction from a suspension comprising a particulate/liquid dispersion through capacitive electrophoresis and capacitive electro-osmosis processes , the method comprising:a) receiving an untreated suspension in an insulated container;b) providing at least one pair of high electric capacitance electrodes spaced apart within the container, wherein the electrodes act as electric double layer capacitors when a potential difference is applied between them;c) applying a direct current (DC) potential difference between the electrodes to generate an electric field across the suspension to drive capacitive electro-kinetic dewatering, wherein the electric potential developed between each electrode and the adjacent suspension is less than the potential needed to cause occurrence of faradic reactions at the electrodes;d) reversing the polarity of the DC potential applied to the electrodes upon approach of the initiation of faradic reactions, resulting in the reversal of the electric field direction between electrodes and prevention of faradic reactions at the electrodes; ande) removing fluid driven from the suspension with at least one outlet drain line.2. The method of , wherein the method is operated as either a batch or a continuous process , the method further comprising initially transporting ...

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04-03-2021 дата публикации

CAPILLARY BARRIERS FOR STAGED LOADING OF MICROFLUIDIC DEVICES

Номер: US20210062181A1
Принадлежит:

Various aspects of the present disclosure are directed toward methods and apparatuses for interacting a first liquid and a second liquid in one or more fluidic channels of a capillary structure. The methods and apparatuses can include providing at least one capillary barrier that positions a meniscus of the first liquid at a fluid-interface region using capillary forces within the capillary structure. Additionally, a path is provided along one of the channels for the second liquid to flow toward the fluid-interface region. Additionally, gas pressure is released, via a gas-outflow port, from the fluid-interface region while flow of the first liquid is arrested. Further, the first liquid and the second liquid contact in the fluid-interface region with the capillary barrier holding the first liquid at the fluid-interface region. 1. An apparatus comprising: '(i) the first microfluidic channel or reservoir comprises a capillary barrier comprising a ramp and an expansion, wherein the expansion is positioned at the fluid-interface region and wherein the capillary barrier is configured and arranged to position a meniscus of the first liquid at the edge of the expansion at the fluid-interface region using capillary forces;', 'a capillary structure comprising (1) a first microfluidic channel or reservoir for guiding a first liquid toward a fluid-interface region and (2) a second microfluidic channel for guiding a second liquid toward the fluid-interface region, whereinan escape path through which fluid can escape from the fluid-interface region; anda gas-outflow port connected to the escape path and configured and arranged to release gas pressure while the second liquid flows toward the fluid-interface region.2. The apparatus of claim 1 , wherein the first microfluidic channel or reservoir and the second microfluidic channel or reservoir are formed from polydimethylsiloxane (PDMS).3. A method for interacting a first liquid and a second liquid in one or more fluidic channels ...

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22-05-2014 дата публикации

GEL ELECTROPHORESIS DEVICE FOR LOADING LARGE SAMPLE VOLUMES

Номер: US20140138249A1

A device for gel electrophoresis is provided having larger wells for loading an increased sample volume with improved gel resolution. The device includes a gel cassette having a front plate and a back plate, wherein at least one plate has a stepped inner surface to create a wider opening at the top of the gel cassette, a gel matrix, and a comb with teeth having a thickness substantially equal to the spacing at the top opening of the gel cassette. Using this device, the sample volume in each well can be increased and the sample height in each well can be significantly reduced, as compared to loading the same volume in the wells of a standard gel cassette. 1. A device for performing gel electrophoresis , the device comprising:(i) a gel cassette comprising a front plate and a back plate, the front plate and the back plate each having an inner surface, a top edge, a bottom edge and two side edges, the front and back plates being fastened together along at least the two side edges, such that the inner surfaces are face-to-face with a spacing there between forming a gel chamber, wherein the inner surface of at least one of the front and back plates has a stepped configuration such that the at least one plate has a top portion and a bottom portion, wherein the top portion has a thickness at least 0.05 mm thinner than the bottom portion, and the spacing between the plates is greater at the top portion than the spacing between the plates at the bottom portion;(ii) a gel matrix held within the gel chamber; and(iii) a comb inserted in the spacing at the top portion of the plates, the comb having a spine and at least one tooth extending from the spine downwardly into the gel matrix to form wells in the gel matrix, the at least one tooth having a thickness substantially equal to the spacing between the top portions of the plates, such that upon removal of the comb for performing the gel electrophoresis, the wells are bounded on opposite sides by the top portions of the plates.2. ...

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20-02-2020 дата публикации

Capillary Electrophoresis Apparatus and Thermostat

Номер: US20200057022A1
Принадлежит:

The capillary electrophoresis apparatus according to the present invention can maintain the temperature in the longitudinal direction of each of a plurality of capillaries uniformly, such that the separation performance of the capillary electrophoresis apparatus can be stabilized, and the analysis performance can be improved. A capillary electrophoresis apparatus according to the present invention includes: a thermostat having a heat source, a first heat conduction member, and a detection window for detecting a sample, the thermostat being configured to maintain a capillary at a predetermined temperature; a capillary holder having a second heat conduction member for sandwiching the capillary between the second heat conduction member and the first heat conduction member, the capillary holder being configured to hold the capillary; and a detection unit configured to detect a sample to be electrophoresed in the capillary, wherein in the heat source, a heat generation amount of at least one of a periphery of the detection window and an end of the capillary is higher than a heat generation amount of another portion. 1. A capillary electrophoresis apparatus , comprising:a thermostat having a heat source, a first heat conduction member, and a detection window for detecting a sample, the thermostat being configured to maintain a capillary at a predetermined temperature;a capillary holder having a second heat conduction member for sandwiching the capillary between the second heat conduction member and the first heat conduction member, the capillary holder being configured to hold the capillary; anda detection unit configured to detect a sample to be electrophoresed in the capillary,wherein in the heat source, a heat generation amount of at least one of a periphery of the detection window and an end of the capillary is higher than a heat generation amount of another portion.2. The capillary electrophoresis apparatus according to claim 1 ,wherein the heat generation amount of ...

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29-05-2014 дата публикации

DEVICE FOR MANUFACTURING ORGANIC HYDRIDE

Номер: US20140144774A1
Принадлежит:

The device for electrochemically manufacturing an organic hydride of the present invention is characterized by the electrode structure thereof being a structure that forms a matrix in which a metal-catalyst supporting carbon or a metal catalyst is suitably intermingled with a proton-conductive solid polymer electrolyte as catalyst layers, and the catalyst layers are formed on the front and back of a proton-conductive solid polymer electrolyte membrane on which a layer that blocks water from passing through is formed. When water or water vapor is supplied to the anode side of this electrode and a substance to be hydrogenated is supplied to the cathode side, application of a voltage between the anode and the cathode causes an electrolysis reaction to the water to occur at the anode and a hydrogenation reaction to the substance to be hydrogenated to occur at the cathode, producing the organic hydride. 1. A device for manufacturing an organic hydride comprising:a membrane electrode assembly including a cathode catalyst layer that reduces a substance to be hydrogenated, an anode catalyst layer that oxidizes water, and a proton-conductive solid polymer electrolyte membrane disposed between the cathode catalyst layer and the anode catalyst layer;a member that supplies the substance to be hydrogenated to the cathode catalyst layer; anda member that supplies water or water vapor to the anode catalyst layer;wherein a layer that blocks water is formed on a surface or inside of the solid polymer electrolyte membrane.2. The device for manufacturing an organic hydride according to claim 1 ,wherein the layer that blocks water includes palladium or a palladium alloy.3. The device for manufacturing an organic hydride according to claim 1 ,wherein the layer that blocks water includes an organic polymer having an amount of ion exchange of 0.75 meq/g or less per dry weight.4. The device for manufacturing an organic hydride according to claim 1 ,wherein the cathode catalyst layer ...

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29-05-2014 дата публикации

Method of producing graphane and graphane-like materials

Номер: US20140144787A1
Принадлежит:

The invention relates to nanotechnology and to producing graphane and graphane-like materials and associated structures, which can be used to create hydrogen fuel cell energy, particularly for transportation systems as well as for creating nanoelectronic systems, based on the use of graphene with controllable electronic properties. The method includes grapheme, or several layers of graphene, placed in water or electrolytic solution, an anode, a cathode, and an adjustable voltage source, where the graphene's potential is lower than the anode's potential. The technical result is an increase in the rate of hydrogenation reactions, which simplifies and lowers the cost of technologies necessary for producing graphane fuel cells and creating conditions to enable their mass production. 1. A method of producing graphane and graphane-like material, comprising: a hydrogen medium, anode, cathode, and target, consisting of N-layered graphene, positioned in the space between the anode and cathode, electrically connected to the cathode, implementing the use of water or electrolytic solution as a hydrogen-containing medium, where the graphene target is located in such water or hydrogen-containing medium and has a potential lower than the anode potential. The invention relates to methods of producing hydrogenated single-layered and N-layered graphene and hydrogen-containing graphene nanostructures (graphane-like materials), which are considered to have prospective applications in the areas of electronics and hydrogen power and/or energy, particularly as hydrogen fuel cells for electric vehicles.There exists a known method for producing fully hydrogenated graphene (graphane) using ion-plasma treatment on graphene (Novoselov K. S., Geim A. K., Morozov S. V., Jiang D., Zhang Y., Dubonos S. V., Grigorieva I. V., Firsov A. A, Electric field effect in atomically thin carbon films. Science 306, 666 (2004)). The essence of the method is to place a graphene sample into a vacuum chamber that ...

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09-03-2017 дата публикации

HYDROLYSIS-RESISTANT POLYACRYLAMIDE GELS

Номер: US20170067852A1
Принадлежит:

Polyacrylamide gels that offer high resolution in protein separations and are more stable relative to hydrolysis than conventional polyacrylamide gels that rely on Tris or Tris-Bis as buffering agents are made by incorporating triethanolamine in place of most or all of the Tris or Tris-Bis. 1. A polyacrylamide gel comprising:crosslinked polyacrylamide;triethanolamine;a catalytic amount of a polymerization catalyst selected from the group consisting of ammonium persulfate, N,N′-tetramethylenediamine (TEMED), riboflavin, β-dimethylamino-propionitrile, and combinations thereof;either no or about 0.3 mM or less of tris(hydroxymethyl)aminomethane; andeither no or about 0.3 mM or less of g/L bis(2-hydroxyethyl)amino-tris(hydroxymethyl)methane;wherein the polyacrylamide gel has a pH of from about 6.4 to about 7.0, and wherein the polyacrylamide gel is configured to separate and resolve proteins under electrophoretic conditions.2. (canceled)3. The polyacrylamide gel of wherein said triethanolamine constitutes from about 50 mM to about 150 mM of said gel.4. The polyacrylamide gel of wherein said gel is devoid of tris(hydroxymethyl)aminomethane and devoid of bis(2-hydroxyethyl)amino-tris(hydroxymethyl)methane.57-. (canceled)8. The polyacrylamide gel of further comprising an ampholyte.9. The polyacrylamide gel of wherein said ampholyte is glycine at a concentration of from about 50 mM to about 500 mM.10. The polyacrylamide gel of further comprising a conjugate ampholyte at a proportion of from about 0.1 mole percent to about 65 mole percent relative to said glycine.1112-. (canceled)13. The polyacrylamide gel of wherein said conjugate ampholyte is taurine and is at a proportion of from about 20 mole percent to about 60 mole percent relative to said glycine.14. The polyacrylamide gel of further comprising a weak acid at a concentration of from about 10 mM to about 500 mM.15. (canceled)16. A method for separating a mixture of proteins by electrophoresis claim 9 , said method ...

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07-03-2019 дата публикации

Method for the fabrication of corrosion resistant electrodes

Номер: US20190072517A1
Автор: Trainoff Steven P.
Принадлежит: WYATT TECHNOLOGY CORPORATION

An electrode for use in instruments capable of measuring the electrophoretic mobility of particles in solution is disclosed. The electrode is comprised of an inexpensive support member, generally made of titanium, onto a flat surface of which has been connected, generally by microwelding, a flat electrically conductive but chemically inert foil member, preferably platinum. A uniform texture can be generated on the exposed surfaces of the electrode by various means including tumbling the electrode with an abrasive. An oxide layer can be generated on the support member by soaking the composite electrode in an appropriate medium, protecting the exposed surface of the support member from fluid contact with the sample solution, while the foil member, unaffected by the oxidation process, is able to contact the sample solution. 1. A method comprising:providing an electrically conductive support member comprising at least one planar surface;providing a flat, chemically inert, electrically conductive foil member;welding the foil member to the conductive support member about the perimeter of the foil member, forming, thereby, a composite electrode, wherein the welding is performed by an electron beam;etching an exposed surface of the foil member of the composite electrode so as to achieve a uniform, non-smooth surface there upon, wherein the etching comprises applying hydrofluoric acid to the composite electrode; andin response to the etching, exposing the composite electrode to an oxidation agent, thereby causing an oxide layer to be formed upon an exposed surface of the support member.2. The method of wherein the foil member comprises platinum.3. The method of wherein the foil member is a disc of platinum foil.4. The method of further comprising milling an o-ring groove about a perimeter of the support member wherein the perimeter of the support member is chosen such that the perimeter does not intersect the planar surface to which the foil member is attached.5. The method ...

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05-06-2014 дата публикации

Method of Producing Coupled Radical Products from Biomass

Номер: US20140154766A1
Принадлежит: Ceramatec, Inc.

A method that produces coupled radical products from biomass. The method involves obtaining a lipid or carboxylic acid material from the biomass. This material may be a carboxylic acid, an ester of a carboxylic acid, a triglyceride of a carboxylic acid, or a metal salt of a carboxylic acid, or any other fatty acid derivative. This lipid material or carboxylic acid material is converted into an alkali metal salt. The alkali metal salt is then used in an anolyte as part of an electrolytic cell. The electrolytic cell may include an alkali ion conducting membrane (such as a NaSICON membrane). When the cell is operated, the alkali metal salt of the carboxylic acid decarboxylates and forms radicals. Such radicals are then bonded to other radicals, thereby producing a coupled radical product such as a hydrocarbon. The produced hydrocarbon may be, for example, saturated, unsaturated, branched, or unbranched, depending upon the starting material. 1. A method for producing a coupled radical product from biomass comprising:obtaining a quantity of biomass;converting the biomass into at least one alkali metal salt of a carboxylic acid, wherein the alkali metal comprises sodium such that the at least one alkali metal salt of the carboxylic acid comprises a sodium salt of the carboxylic acid;preparing an anolyte, wherein the anolyte comprises a quantity of the at least one alkali metal salt of the carboxylic acid; anddecarboxylating the at least one alkali metal salt of the carboxylic acid within the anolyte, wherein the decarboxylating converts the at least one alkali metal salt of the carboxylic acid into alkyl radicals that react to form a coupled radical product comprising an alkyl radical coupled to another alkyl radical or to a hydrogen radical.2. A method as in claim 1 , wherein the coupled radical product comprises a hydrocarbon.3. A method as in claim 1 , wherein the converting the biomass comprises saponification claim 1 , wherein a base is reacted with a quantity of the ...

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26-03-2015 дата публикации

Molten Carboxylate Electrolytes for Electrochemical Decarboxylation Processes

Номер: US20150083606A1
Автор: Bhavaraju Sai, Mosby James
Принадлежит:

Molten salt electrolytes are described for use in electrochemical synthesis of hydrocarbons from carboxylic acids. The molten salt electrolyte can be used to synthesize a wide variety of hydrocarbons with and without functional groups that have a broad range of applications. The molten salt can be used to synthesize saturated hydrocarbons, diols, alkylated aromatic compounds, as well as other types of hydrocarbons. The molten salt electrolyte increases the selectivity, yield, the energy efficiency and Coulombic efficiency of the electrochemical conversion of carboxylic acids to hydrocarbons while reducing the cell potential required to perform the oxidation. 1. An electrochemical cell comprising:an electrolyte compartment with a quantity of electrolyte, the electrolyte comprising a quantity of an inorganic salt of a carboxylic acid dissolved in a molten salt electrolyte;an anode in communication with the electrolyte;a cathode in communication with the electrolyte; anda voltage source that decarboxylates the metal salt of the carboxylic acid into radicals that react to form at least one radical coupling product.2. The cell of claim 1 , wherein the cation of the electrolyte inorganic salt is selected from an alkaline metal claim 1 , an alkaline earth metal claim 1 , and mixtures of the same.3. The cell of claim 1 , wherein the cation of the electrolyte inorganic salt is selected from ammonium claim 1 , lithium claim 1 , sodium claim 1 , potassium claim 1 , magnesium claim 1 , calcium claim 1 , and mixtures of the same.4. The cell of claim 2 , wherein the electrolyte contains a mixture of inorganic cations.5. The cell of claim 2 , wherein the electrolyte contains a mixture of at least three inorganic cations.6. The cell of claim 2 , wherein the oxidation potential of an anion in the molten electrolyte is higher than the oxidation potential of the carboxylate anion.7. The cell of claim 2 , wherein a carboxylate portion of the carboxylate inorganic salt is selected from: ...

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14-03-2019 дата публикации

CAPILLARY BARRIERS FOR STAGED LOADING OF MICROFLUIDIC DEVICES

Номер: US20190078077A1
Принадлежит:

Various aspects of the present disclosure are directed toward methods and apparatuses for interacting a first liquid and a second liquid in one or more fluidic channels of a capillary structure. The methods and apparatuses can include providing at least one capillary barrier that positions a meniscus of the first liquid at a fluid-interface region using capillary forces within the capillary structure. Additionally, a path is provided along one of the channels for the second liquid to flow toward the fluid-interface region. Additionally, gas pressure is released, via a gas-outflow port, from the fluid-interface region while flow of the first liquid is arrested. Further, the first liquid and the second liquid contact in the fluid-interface region with the capillary barrier holding the first liquid at the fluid-interface region. 1. An apparatus comprising: '(i) the first microfluidic channel or reservoir comprises a capillary barrier comprising a ramp and an expansion, wherein the expansion is positioned at the fluid-interface region and wherein the capillary barrier is configured and arranged to position a meniscus of the first liquid at the edge of the expansion at the fluid-interface region using capillary forces;', 'a capillary structure comprising (1) a first microfluidic channel or reservoir for guiding a first liquid toward a fluid-interface region and (2) a second microfluidic channel for guiding a second liquid toward the fluid-interface region, whereinan escape path through which fluid can escape from the fluid-interface region; anda gas-outflow port connected to the escape path and configured and arranged to release gas pressure while the second liquid flows toward the fluid-interface region.2. The apparatus of claim 1 , wherein the first microfluidic channel or reservoir and the second microfluidic channel or reservoir are formed from polydimethylsiloxane (PDMS).3. The apparatus of claim 1 , wherein the first microfluidic channel or reservoir and the second ...

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24-03-2016 дата публикации

LEAK-PROOF GEL CASTING DEVICE FOR VERTICAL PROTEIN ELECTROPHORESIS SYSTEM

Номер: US20160084797A1
Принадлежит:

Disclosed is a leak-proof gel casting device for a vertical protein electrophoresis system which includes a gel caster which has a plurality of compartments and a sealing pad which is inserted to the compartments of the gel cater, and is formed to wrap around both sides and an edge of a lower side of a plate set. The present invention with a simple structure is capable of being manufactured at a low cost, does not require frequent replacement because of its long-term durability, and is highly effective in preventing the leakage of the gel placed in the gap between the plates. 1. A leak-proof gel casting device for a vertical protein electrophoresis system , the device comprising:a gel caster configured to have a plurality of compartments; anda sealing pad configured to be inserted to the compartments of the gel cater, the sealing pad being formed to wrap around both sides and an edge of a lower side of a plate set.2. The leak-proof gel casting device as claimed in claim 1 , wherein the compartment of the gel caster is in a shape of “”.3. The leak-proof gel casting device as claimed in claim 1 , wherein the gel caster is made of acrylic claim 1 , synthetic resin claim 1 , glass claim 1 , or metal.4. The leak-proof gel casting device as claimed in claim 1 , wherein the plurality of compartments of the gel caster are disposed in front claim 1 , back claim 1 , left claim 1 , and right directions on a plane or have a stepped structure.5. The leak-proof gel casting device as claimed in claim 1 , wherein the sealing pad is in a shape of “” in accordance with the shape of the compartments.6. The leak-proof gel casting device as claimed in claim 5 , wherein a cross-sectional shape of the sealing pad is in a shape of “” claim 5 , and at both inner sides of the sealing pad claim 5 , projections form a line around the edge.7. The leak-proof casting device as claimed in claim 5 , further comprising: a supplementary part in any side of outer sides of the sealing pad.8. The leak- ...

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31-03-2022 дата публикации

NUCLEIC ACID DETECTION KIT AND NUCLEIC ACID DETECTION DEVICE

Номер: US20220099622A1
Принадлежит:

A nucleic acid detection kit includes a detection chip, an electrophoresis box, and a barrier unit. The detection chip includes a channel and an outlet. The outlet is connected to the channel. The temperature-dependent barrier unit may be in a first state or a second state. The barrier unit is disposed on a side of the outlet close to the channel when the barrier unit is in the first state, so that the channel is disconnected from the electrophoresis box. The barrier unit is away from the outlet when the barrier unit is in the second state, so that the channel is connected to the electrophoresis box. A nucleic acid detection device including the nucleic acid detection kit is also disclosed. The nucleic acid detection device has a simple structure, which is portable, flexible, and convenient, and can be used at home. 1. A nucleic acid detection kit , comprising:a detection chip;an electrophoresis box; anda barrier unit;wherein the detection chip comprises a first cover plate, a spacer layer, and a second cover plate, two opposite surfaces of the spacer layer are respectively in contact with the first cover plate and the second cover plate, the first cover plate, the spacer layer, and the second cover plate cooperatively define a channel, the first cover plate defines an outlet, the outlet is connected to the channel;wherein the barrier unit is in a first state or a second state, the barrier unit is disposed on a side of the outlet close to the channel when the barrier unit is in the first state, so that the channel is disconnected from the electrophoresis box, the barrier unit is away from the outlet when the barrier unit is in the second state, so that the channel is connected to the electrophoresis box.2. The nucleic acid detection kit of claim 1 , wherein the electrophoresis box comprises a connection unit claim 1 , the channel is connected to the electrophoresis box through the connection unit claim 1 , the connection unit comprises a first end claim 1 , and the ...

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31-03-2022 дата публикации

Applicator comb with serrated teeth for gel electrophoresis

Номер: US20220099625A1
Автор: Tipton L Golias
Принадлежит: Helena Laboratories Corp

An applicator having a body and a series of teeth extending in a first direction from said body, the teeth spaced apart from each other in a second direction perpendicular to said first direction, each tooth having a base, a tip, and opposed sides, in which at least one tooth includes a series of serrations spaced apart from each other, each serration having a tip, and a base, and opposed sides and preferably being of generally trapezoidal shape. A method for depositing a liquid sample on a substrate using the applicator.

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02-04-2015 дата публикации

FITTING FOR ELASTICALLY-BIASING A CAPILLARY FOR A FLUID-TIGHT CONNECTION TO A FLUIDIC CONDUIT

Номер: US20150090595A1
Автор: Lueth Claus, Zeko Darijo
Принадлежит: aGILENT TECHNOLOGIES , INC.

A fitting () for providing a fluid connection between a capillary () and a fluidic conduit () of a fluidic component (), the fitting () comprising a male piece () and a female piece () for connection with the male piece (), wherein the male piece () comprises a housing () with a capillary reception () configured for receiving the capillary (), wherein a part of the capillary () being received in the capillary reception () is circumferentially covered by a sleeve (), an elastic biasing mechanism () being arranged at least partially within the housing (), being configured for biasing the capillary () against the female piece () and being supported by the sleeve (), and a locking mechanism () being arranged at least partially within the housing () and being configured for locking the capillary () to the fitting (). 1. A fitting for providing a fluid connection between a capillary and a fluidic conduit of a fluidic component , the fitting comprising a male piece and a female piece for connection with the male piece , the male piece comprising:a housing with a capillary reception configured for receiving the capillary, wherein a part of the capillary being received in the capillary reception is circumferentially covered by a sleeve;an elastic biasing mechanism being arranged at least partially within the housing, being configured for biasing the capillary against the female piece and being supported by the sleeve; anda locking mechanism being arranged at least partially within the housing and being configured for locking the capillary to the fitting.2. The fitting according to claim 1 , wherein the elastic biasing mechanism is supported by an annular flange face of the sleeve claim 1 , particularly by an annular flange face at a back side of the sleeve.3. The fitting according to claim 1 , wherein the locking mechanism is configured for locking the capillary at an annular flange face of the sleeve claim 1 , particularly by an annular flange face at a back side of the ...

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26-06-2014 дата публикации

Systems and Methods of Improved Fermentation

Номер: US20140174944A1

Devices, systems and methods for processing cellulosic material to produce fermentable sugars are provided. Devices, systems and methods for increasing fermentation rates of microbes via biostimulation are provided. Electrodes are preferably positioned along an interior or exterior of a tube-shaped component to administer electromagnetic/electric pulses to a solution comprising a microbe. Systems can advantageously be used in new biofuels production plants, or in existing biofuels production plants without the need for significant retrofits. 1. A method of processing a cellulosic material to produce a sugar , comprising:using a multi-frequency electrical signal to generate free radicals at a first electrode; andusing the free radicals to electrolyze compounds with the cellulosic material.2. The method of claim 1 , wherein the multi-frequency electrical signal is produced using an electrical arc.3. The method of claim 1 , wherein the multi-frequency electrical signal is produced using a plasma generator.4. The method of claim 1 , wherein the multi-frequency electrical signal has a highest frequency and a lowest frequency claim 1 , and the highest frequency is at least ten times the lowest frequency.5. The method of claim 1 , wherein the multi-frequency electrical signal has a highest frequency and a lowest frequency claim 1 , and the highest frequency is at least a hundred times the lowest frequency.6. The method of claim 1 , wherein the first electrode is used as an anode and has a metal selected from a first group consisting of PMG metals.7. The method of claim 6 , wherein the first electrode cooperates with a cathode comprising a metal selected from a second group consisting of transition metals.8. The method of claim 1 , wherein the step of using the free radicals occurs at least in part at a pressure of less than 2 Bar.9. The method of claim 1 , wherein the step of using the free radicals occurs at least in part at a temperature of less than 200° C.10. The ...

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16-04-2015 дата публикации

Side-Eluting Molecular Fractionator

Номер: US20150101932A1
Принадлежит:

The disclosure provides cassettes, electrophoresis systems, methods for making the device, and methods of fractionating a sample using the cassettes and electrophoresis systems described herein. 1. An electrophoresis cassette comprising:a top plate comprising at least one macrofluidic separation channel, at least one of an opening, a cavity, or a recess corresponding to a portion of a positive elution channel, at least one of an opening, a cavity, or a recess corresponding to a portion of a negative elution channel, and at least one elution module, anda bottom plate,wherein the top plate and bottom plate are contacted or adhered to one another.2. The cassette of claim 1 , wherein the bottom plate comprises at east one of are opening claim 1 , a cavity claim 1 , of a recess corresponding to a portion of a positive elution channel claim 1 , at least one of an opening claim 1 , cavity claim 1 , or a recess corresponding to a portion a negative elution channel claim 1 , and at least one of an opening claim 1 , cavity claim 1 , or a recess corresponding to the elution module.3. The cassette of claim 2 , wherein the contacting or adhesion of the top plate and the bottom plate forms at least one positive elution channel and at least one negative elution charnel.4. The cassette of claim 1 , wherein the elution module comprisesa four-sided structure having a solid bottom surface, a top surface comprising an elution port, and two side surfaces each having at least one of an opening, cavity, or a recess corresponding to at least one positive elution channel or at least one negative elution channel, andan analyte-impermeable membrane in contact with at least one side of the structure.5. The cassette of claim 4 , wherein the two side surfaces taper from a wider configuration near the top surface to a narrower configuration near the bottom surface.6. The cassette of claim 4 , wherein analyte-impermeable membrane contacts or adheres to the side of the structure adjacent to at ...

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14-04-2016 дата публикации

SYSTEM AND METHOD FOR THE CONCENTRATION OF A SLURRY

Номер: US20160101388A1
Принадлежит: Omya International AG

The present invention relates to a system and a method for the concentration of slurry, especially mineral containing slurry. The invention provides a system comprising an electrophoresis unit and a separation unit, where the separation unit comprises a recipient, preferably of half cylindrical form, with adjusted flanks for separating the solid material or cake from the rotating anodes and a sliding carriage suitable for closing the recipient and stripping resting solid material from the flanks into the recipient before the solid material or cake is pressed out of the recipient by a piston. 1. A method for the concentration of a slurry of dispersed particles using a device with a supporting structure including modules received therein , the modules comprising:a. an electrophoresis cell with at least one electrically connected cathode, and at least one electrically connected, rotatable anode disc,b. a separation unit adjacent to each anode surface for receiving a cake material of dispersed particles, comprising a recipient and a piston adapted to the recipient form, wherein the recipient has shoulders dimensioned to act as scraping flanges for taking off the cake material from each anode, the piston is dimensioned for pressing the cake material out of the recipient, the separation unit has a sliding carriage with a cover for closing the recipient and collecting residual cake material from the shoulders into the recipient while closing it, and the recipient has a half cylindrical form,the method comprising the following steps:(i) introducing a slurry with dispersed particles in the electrophoresis cell of the device;(ii) applying voltage to the resting electrodes of the electrophoresis cell;(iii) rotating each anode a defined angle of rotation and stripping resting solid material or cake into the recipient of the separation unit of the device;(iv) optionally closing the recipient with the sliding carriage;(v) pressing the solid material or cake out of the separation ...

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14-04-2016 дата публикации

HIGH SPEED, HIGH RESOLUTION COMPOSITIONS, METHODS AND KITS FOR CAPILLARY ELECTROPHORESIS

Номер: US20160103097A1
Принадлежит:

The invention provides compositions, methods and kits for high speed, high resolution of analytes by capillary electrophoresis starting with uncoated capillaries. The compositions comprise a sieving component, comprising a non-crosslinked acrylamide polymer, and a surface interaction component, comprising at least one uncharged and non-crosslinked water-soluble silica-adsorbing polymer. Methods for employing the novel compositions in capillary electrophoresis are provided. Kits comprising the novel compositions for use in the novel methods are also provided. 1. A composition for separating analytes by capillary electrophoresis comprising:a sieving component comprising a non-crosslinked acrylamide polymer having a weight average molecular weight between about 3,000,000 Da and less than or equal to about 6,400,000 Da; and{'sub': 1', '3', '1', '3', '1', '3', '1', '3, 'a surface interaction component comprising one or more non-crosslinked polymers selected from the group consisting of poly(meth)acrylamide, N,N-disubstituted polyacrylamide and N-substituted polyacrylamide, wherein the N-substituents are selected from the group consisting of Cto Calkyl, halo-substituted Cto Calkyl, methoxy-substituted Cto Calkyl, and hydroxyl-substituted Cto Calkyl; wherein the sieving component and the surface interaction component differ in polymer chemical composition; and'}wherein the composition does not include a crosslinked polymer gel and has a viscosity of less than 10,000 centipoise at 25° C.2. (canceled)3. The composition of claim 1 , wherein the composition has a viscosity of less than 5 claim 1 ,000 centipoise at 25° C.4. The composition of claim 1 , wherein the composition has a viscosity of less than 1000 centipoise at 25° C.5. The composition of claim 1 , wherein the composition has a viscosity of less than 600 centipoise at 25° C.6. The composition of claim 1 , wherein the one or more non-crosslinked polymers comprises poly(N claim 1 ,N-dimethylacrylamide).7. The ...

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14-04-2016 дата публикации

ELECTROPHORESIS BUFFER FOR EXTENDING THE USEFUL ELECTROPHORESIS LIFE OF AN ELECTROPHORESIS GEL

Номер: US20160103098A1
Автор: Sevigny Pierre
Принадлежит: DGel Electrosystem Inc.

There is provided an electrolyte solution for extending useful electrophoresis life of an electrophoresis gel containing Tris(hydroxymethyl)aminomethane (TRIS), at least one zwitterion, and water. The electrolyte solution may be used in buffer systems for gel electrophoresis, such as SDS-PAGE. 128.-. (canceled)29. An electrolyte solution for extending a useful electrophoresis life of an electrophoresis gel comprising:at least one zwitterion chosen from 2-amino-2methyl-1,3-propanediol (AMPD), N-(1,1-Dimethyl-2-hydroxyethyl)-3-amino-2-hydroxypropanesulfonic acid (AMPSO), N-Glycylglycine (Gly-Gly), 4-(2-hydroxyethyl)piperazine-1-propanesulfonic acid (EPPS or HEPPS), 3-(cyclohexylamino)-1-propanesulfonic acid (CAPS), 3-(cyclohexylamino)-2-hydroxy-1-propanesulfonic acid (CAPSO), 2-(cyclohexylamino)ethanesulfonic acid (CHES), N,N-bis[2-hydroxyethyl]-2-aminoethanesulphonic acid (BES), (2-[2-hydroxy-1,1-bis(hydroxymethyl)ethylamino]ethanesulphonic acid) (TES), N-Tris(hydroxymethyl)methyl-3-aminopropanesulfonic acid (TAPS), 3-N-Morpholino propanesulfonic acid (MOPS), and Piperazine-N,N′-bis(2-hydroxypropanesulfonic acid) (POPSO);water;Tris(hydroxymethyl)aminomethane (TRIS), wherein the concentration of Tris(hydroxymethyl)aminomethane (TRIS) is from about 10 mM to about 500 mM; anda chelating agent selected from the group consisting of ethylene glycol tetraacetic acid (EGTA), trisodium nitrilotriacetate, hydroxyethyl ethylenediamine trisodium acetate (trisodium HEDTA), diethylene triamino pentasodium acetate or uramil disodium acetate,wherein the pH of the electrolyte solution is from about 8.0 to about 11.0.30. The electrolyte solution of claim 29 , wherein the zwitterion is 4-(2-hydroxyethyl)piperazine-1-propanesulfonic acid (EPPS or HEPPS).31. The electrolyte solution of claim 29 , wherein the zwitterion is chosen from N-Glycylglycine (Gly-Gly) and 3-(cyclohexylamino)-2-hydroxy-1-propanesulfonic acid (CAPSO).32. The electrolyte solution of claim 29 , further comprising ...

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08-04-2021 дата публикации

Object trapping device and object trapping device unit

Номер: US20210101115A1
Принадлежит: Sharp Corp, University of Tokyo NUC

An object trapping device enables efficiently trapping a plurality of objects in a specific combination. Each of a first electrode pair ( 13 ), a second electrode pair ( 14 ), and a third electrode pair ( 15 ) in an electrode pair group ( 3 ) is applied with an individual AC voltage and traps an object by dielectrophoresis generated in accordance with the AC voltage that is applied.

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12-04-2018 дата публикации

Isotachophoresis enhanced isothermal nucleic acid amplification

Номер: US20180100183A1
Принадлежит: UNIVERSITY OF WASHINGTON

The present disclosure relates generally to the integration of isotachophoresis (ITP) and isothermal nucleic acid amplification methods such as recombinase polymerase amplification (RPA). One aspect of the disclosure relates to a method for concentrating and amplifying a nucleic acid, the method including an isotachophoresis device, the isotachophoresis device including a porous matrix, and first and second electrodes, having a leading electrolyte, a trailing electrolyte and a set of isothermal nucleic acid amplification reaction reagents disposed in the porous matrix as described herein, and applying a voltage across the first electrode and the second electrode for a time sufficient to provide a first isotachophoresis (ITP) plug comprising an amplification product of the nucleic acid, wherein the concentration of the nucleic acid is substantially higher in the first FTP plug than in the first and/or second fluids outside of the first ITP plug.

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03-07-2014 дата публикации

NUCLEIC ACID SAMPLE PREPARATION

Номер: US20140183042A1
Принадлежит: Biological Dynamics, Inc.

The present invention includes methods, devices and systems for isolating a nucleic acid from a fluid comprising cells. In various aspects, the methods, devices and systems may allow for a rapid procedure that requires a minimal amount of material and/or results in high purity nucleic acid isolated from complex fluids such as blood or environmental samples. 1. A method for isolating a nucleic acid from a fluid , the method comprising:a. applying the fluid to a device, the device comprising an array of electrodes capable of establishing an AC electrokinetic field region;b. concentrating cells and/or other particulate material in the fluid in a first AC electrokinetic field region, wherein the first AC electrokinetic field region is a dielectrophoretic low field region and the conductivity of the fluid is greater than 300 mS/m;c. isolating nucleic acid in a second AC electrokinetic field region, wherein the second AC electrokinetic field is a dielectrophoretic high field region; andd. flushing the concentrated cells and/or other particulate material from the first AC electrokinetic field region.2. The method of claim 1 , wherein the AC electrokinetic field is produced using an alternating current having a voltage of 1 volt to 40 volts peak-peak claim 1 , and/or a frequency of 5 Hz to 5 claim 1 ,000 claim 1 ,000 Hz and duty cycles from 5% to 50%.3. The method of claim 1 , wherein the conductivity of the fluid is greater than 500 mS/m.4. The method of claim 1 , wherein the electrodes are selectively energized to provide the first dielectrophoretic field region and subsequently or continuously selectively energized to provide the second dielectrophoretic field region.5. The method of claim 1 , wherein the array of electrodes is spin-coated with a hydrogel having a thickness between about 0.1 microns and 1 micron.6. The method of claim 5 , wherein the hydrogel comprises two or more layers of a synthetic polymer.7. The method of claim 5 , wherein the hydrogel has a ...

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23-04-2015 дата публикации

ELECTROPHORESIS BUFFER FOR EXTENDING THE USEFUL ELECTROPHORESIS LIFE OF AN ELECTROPHORESIS GEL

Номер: US20150107997A1
Автор: Sevigny Pierre
Принадлежит: DGel Electrosystem Inc.

There is provided an electrolyte solution for extending useful electrophoresis life of an electrophoresis gel containing Tris(hydroxymethyl)aminomethane (TRIS), at least one zwitterion, and water. The electrolyte solution may be used in buffer systems for gel electrophoresis, such as SDS-PAGE. 128.-. (canceled)30. The electrolyte solution of claim 29 , wherein the zwitterion is chosen from 4-(2-hydroxyethyl)piperazine-1-propanesulfonic acid (EPPS or HEPPS) claim 29 , N-Glycylglycine (Gly-Gly) and 3-(cyclohexylamino)-2-hydroxy-1-propanesulfonic acid (CAPSO).31. The electrolyte solution of claim 29 , wherein the pH is of the electrolyte solution is from about 8.0 to about 11.0.32. The electrolyte solution of claim 29 , further comprising Tris(hydroxymethyl)aminomethane (TRIS).33. The electrolyte solution of claim 29 , further comprising sodium dodecyl sulphate (SDS).34. The electrolyte solution of claim 29 , further comprising a chelating agent having the name: ethylenediaminetetraacetate (EDTA) claim 29 , ethylene glycol tetraacetic acid (EGTA) claim 29 , trisodium nitrilotriacetate claim 29 , hydroxyethyl ethylenediamine trisodium acetate (trisodium HEDTA) claim 29 , diethylene triamino pentasodium acetate or uramil disodium acetate.35. The electrolyte solution of claim 32 , wherein the concentration of Tris(hydroxymethyl)aminomethane (TRIS) is from about 10 mM to about 500 mM.36. The electrolyte solution of claim 35 , wherein the concentration of Tris(hydroxymethyl)aminomethane (TRIS) is from about 10 mM to about 150 mM.37. The electrolyte solution of claim 35 , wherein the concentration of Tris(hydroxymethyl)aminomethane (TRIS) is from about 25 mM to about 150 mM.38. The electrolyte solution of claim 35 , wherein the concentration of Tris(hydroxymethyl)aminomethane (TRIS) is from about 50 mM to about 150 mM.39. The electrolyte solution of claim 35 , wherein the concentration of Tris(hydroxymethyl)aminomethane (TRIS) is from about 75 mM to about 150 mM.40. The ...

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04-04-2019 дата публикации

SEPARATION MEDIUM FOR ELETROPHORESIS, REAGENT KIT FOR ELETROPHORESIS, AND ELETROPHORESIS METHOD

Номер: US20190101508A1
Автор: ARAI Akihiro
Принадлежит: SHIMADZU CORPORATION

Provided is a separation medium for electrophoresis having an improved separation performance without increasing a viscosity. The separation medium for electrophoresis includes a water-soluble cellulose derivative, and a sugar alcohol derived from monosaccharide or disaccharide or a low-molecular-weight polysaccharide. 1. A separation medium for electrophoresis comprising:a water-soluble cellulose derivative; anda sugar alcohol derived from monosaccharide or disaccharide, or a low-molecular-weight polysaccharide.2. The separation medium for electrophoresis according to claim 1 , wherein the sugar alcohol comprises mannitol claim 1 , erythritol claim 1 , xylitol claim 1 , lactitol claim 1 , maltitol and sorbitol claim 1 , or a combination thereof.3. The separation medium for electrophoresis according to claim 1 , wherein a weight-average molecular weight of the polysaccharide is 10000-80000.4. The separation medium for electrophoresis according to claim 1 , wherein the polysaccharide comprises pullulan claim 1 , agarose claim 1 , dextran claim 1 , dextrin claim 1 , amylose claim 1 , xanthan gum claim 1 , mannan claim 1 , galactomannan claim 1 , gellan gum claim 1 , carrageenan claim 1 , curdlan claim 1 , pectine claim 1 , welan gum claim 1 , alginic acid claim 1 , alginic acid salt claim 1 , alginic acid ester claim 1 , karaya gum claim 1 , tamarind seed gum claim 1 , rhamsan gum claim 1 , or a combination thereof.5. The separation medium for electrophoresis according to claim 1 , wherein the sugar alcohol or the polysaccharide comprises mannitol claim 1 , pullulan claim 1 , or a combination thereof.6. The separation medium for electrophoresis according to claim 1 , wherein the water-soluble cellulose derivative comprises a repeating unit of cellulose claim 1 , in which at least one hydrogen atom of a hydroxyl group is substituted by a substituent which is selected from a group consisting of an alkyl group having 1 to 3 carbon atoms claim 1 , a hydroxyalkyl group ...

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21-04-2016 дата публикации

HIGHLY AUTOMATED CAPILLARY ELECTROPHORESIS SYSTEM

Номер: US20160109474A1
Принадлежит:

The invention is an improved multiplex capillary electrophoresis instrument or module with at least four and preferably six user-accessible vertically stacked drawers. An x-z stage moves samples from the user accessible drawers to the capillary array for analysis. An additional mechanical stage moves the array from side-to-side. The x-z stage, coupled with the additional array stage allows the system to sample all wells of a 384 well plate with a 96-capillary array. A computer program allows users to add capillary electrophoresis jobs to a queue corresponding to the analysis of rows or plates of samples without stopping or interrupting runs in progress. 1. An Electrophoresis console for sampling a 384 well plate in a 96-capillary array system , comprising:a console housing an operable multiplexed capillary electrophoresis system which includes;a 96-capillary array, comprising;a first motion control system built within said console to move a 384-well plate forward and afta second motion control system built within said console to move said 96-well capillary array left and right, perpendicular to the motion of said first motion control system;wherein the combined motion of said first motion control system and said second motion control system allows access to all 394 wells of said 384 well plate with said 96 well capillary array.2. The electrophoresis console of claim 1 , which includes a plurality of externally accessible drawers for holding sample plates or buffer plates in said console; andsaid first motion control system moves sample plates or buffer plates from said drawers to an injection position of said capillary electrophoresis system.3. The electrophoresis console of which includes as the externally accessible drawers at least four vertically stacked drawers.4. The electrophoresis console according to which includes as the externally accessible drawers at least six vertically stacked drawers.5. The electrophoresis console of wherein samples can be placed ...

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20-04-2017 дата публикации

ELECTROPHORESIS GEL WITH EXTENDED SHELF LIFE AND HIGH PERFORMANCE

Номер: US20170108466A1
Принадлежит:

An electrophoresis gel with a gel buffer that includes a gel amine buffer, a primary gel ampholyte, and a conjugate gel ampholyte is disclosed herein. The conjugate gel ampholyte may be selected from threonine and serine. 1. A polyacrylamide gel for gel electrophoresis , comprising: a gel amine buffer;', 'a primary gel ampholyte; and', 'a conjugate gel ampholyte that is selected from threonine and serine., 'a gel buffer comprising2. The polyacrylamide gel of claim 1 , wherein the gel amine buffer is selected from bis(2-hydroxyethyl)-amino-tris(hydroxymethyl)-methane claim 1 , 1 claim 1 ,3-bis(tris(hydroxymethyl)methylamino)propane claim 1 , tris(hydroxymethyl)aminomethane claim 1 , and triethaniolamine.3. The polyacrylamide gel of claim 1 , wherein the gel amine buffer is tris(hydroxymethyl)aminomethane.4. The polyacrylamide gel of claim 1 , wherein the primary gel ampholyte is selected from glycine and tricine.5. The polyacrylamide gel of claim 1 , wherein the primary gel ampholyte is glycine.6. The polyacrylamide gel of claim 1 , wherein the conjugate gel ampholyte is threonine.7. The polyacrylamide gel of claim 1 , wherein the conjugate gel ampholyte is serine.8. The polyacrylamide gel of claim 1 , wherein the concentration of the gel amine buffer is less than about 150 mM.9. The polyacrylamide gel of claim 1 , wherein the concentration of the gel amine buffer ranges from about 40 mM to about 150 mM.10. The polyacrylamide gel of claim 1 , wherein the concentration of the gel amine buffer ranges from about 70 mM to about 140 mM.11. The polyacrylamide gel of claim 1 , wherein the concentration of the gel amine buffer ranges from about 70 mM to about 80 mM.12. The polyacrylamide gel of claim 1 , wherein the concentration of the primary gel ampholyte ranges from about 25 mM to about 250 mM.13. The polyacrylamide gel of claim 1 , wherein the concentration of the primary gel ampholyte ranges from about 100 mM to about 250 mM.14. The polyacrylamide gel of claim 1 , ...

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28-04-2016 дата публикации

MULTI-CHANNEL FLOW DIRECTION CONTROLLER FOR FREE-FLOW ELECTROPHORESIS APPARATUS

Номер: US20160116437A1
Принадлежит:

A multi-channel flow direction controller for a free-flow electrophoresis apparatus, including a base and a propulsion member. The base is provided with a plurality of convex pieces. The propulsion member is provided with a plurality of narrow pieces. The convex pieces and the narrow pieces have the same length and number. One end of the base is provided with a groove, and one end of the propulsion member is disposed in the groove. 1. A multi-channel flow direction controller for a free-flow electrophoresis apparatus , the controller comprising: 'b) a propulsion member, the propulsion member comprising a plurality of narrow pieces;', 'a) a base, the base comprising a plurality of convex pieces; and'} the convex pieces and the narrow pieces have the same length and the same number;', 'one end of the base is provided with a groove, and one end of the propulsion member is disposed in the groove., 'wherein'}2. The controller for claim 1 , further comprising a fixing piece for the propulsion member and a first screw claim 1 , the fixing piece for the propulsion member comprising a first through hole; wherein one end of the base is provided with a first threaded hole; the first through hole and the first threaded hole are integrated via the first screw.3. The controller for claim 2 , further comprising a second screw; wherein another end of the base is provided with a second threaded hole; another end of the propulsion member is provided with a second through hole; the second through hole and the second threaded hole are integrated via the second screw.4. The controller for claim 3 , wherein a rotational mode of the second screw is a manual mode or an automatic mode. This application is a continuation-in-part of International Patent Application No. PCT/CN2013/084949 with an international filing date of Oct. 10, 2013, designating the United States, now pending, and further claims priority benefits to Chinese Patent Application No. 201310270196.1 filed Jun. 28, 2013. The ...

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09-04-2020 дата публикации

NANOCARBON SEPARATION METHOD, NANOCARBON PURIFICATION METHOD, AND DISPERSION LIQUID

Номер: US20200108347A1
Автор: NIHEY Fumiyuki
Принадлежит: NEC Corporation

A nanocarbon separation method includes a step of preparing a dispersion liquid having nanocarbons dispersed therein; a step of injecting a liquid including the dispersion liquid into an electrophoresis tank so that a pH of the liquid increases from a bottom to a top in a direction of gravitational force; and a step of applying a direct current to electrodes disposed in an upper part and a lower part of the electrophoresis tank. 1. A nanocarbon separation method comprising:a step of preparing a dispersion liquid having nanocarbons dispersed therein;a step of injecting a liquid including the dispersion liquid into an electrophoresis tank so that a pH of the liquid increases from a bottom to a top in a direction of gravitational force; anda step of applying a direct current to electrodes disposed in an upper part and a lower part of the electrophoresis tank.2. A nanocarbon separation method comprising:a step of injecting a dispersion liquid having nanocarbons dispersed therein into an electrophoresis tank;a step of injecting a liquid having a pH higher than a pH of the dispersion liquid into the electrophoresis tank after injecting the dispersion liquid; anda step of applying a direct current to electrodes disposed in an upper part and a lower part of the electrophoresis tank.3. The nanocarbon separation method according to claim 2 , wherein the liquid having the pH higher than the pH of the dispersion liquid is a dispersion liquid having the nanocarbons dispersed therein.4. The nanocarbon separation method according to claim 1 , wherein the liquid contains an additive other than the nanocarbons.5. The nanocarbon separation method according to claim 4 , further comprising:a step of preparing a plurality of liquids in which concentrations of the additives are different,wherein the step of injecting the liquid includes:a first injection step of injecting a liquid in which a concentration of the additive is low; anda second injection step of injecting a liquid in which a ...

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07-05-2015 дата публикации

NANOCOMPOSITE POLYMER HYDROGEL WITH ALIGNED NANOPARTICLES

Номер: US20150122652A1
Принадлежит: Tennessee Technological University

Nanocomposite polymeric hydrogels comprising polyacrylamide (PAAm) formulated in combination with magnetically-susceptible anisotropic microparticles are described, as are method of making and using said hydrogels. 1. A hydrogel comprising polyacrylamide and anisotropic nanoparticles wherein said nanoparticles are aligned.2. The hydrogel of claim 1 , wherein said nanoparticles are aligned by an applied magnetic field of at least about 0.5 Tesla claim 1 , or by an applied AC electric field of between about 50 and about 400 Hz and between about 0.1 and about 10 kV/cm claim 1 , or by an applied DC electric field of between about 0.1 and about 10 kV/cm.3. The hydrogel of claim 1 , wherein said nanoparticles are aligned by an applied magnetic field of from about 1 to about 3 Tesla.4. The hydrogel of claim 1 , wherein said nanoparticles are selected from the group consisting of magnetically and/or electrically susceptible anisotropic smectites claim 1 , phyllosilicates claim 1 , clays claim 1 , micas claim 1 , chlorites claim 1 , bentonite claim 1 , antigorite claim 1 , chrysolite claim 1 , lizardite claim 1 , halloysite claim 1 , kaolinite claim 1 , illite claim 1 , vermiculite claim 1 , talc claim 1 , palygorskite claim 1 , pyrophylite claim 1 , biotite claim 1 , muscovite claim 1 , phlogopite claim 1 , lepidolite claim 1 , margarite claim 1 , glauconite claim 1 , chlorite claim 1 , laponite claim 1 , layered double hydroxides claim 1 , iron oxide claim 1 , fibrous nanoparticles claim 1 , and combinations thereof.5. The hydrogel of claim 1 , wherein said nanoparticles are exfoliated montmorillonite nanoparticles.6. The hydrogel of claim 2 , wherein said nanoparticles are exfoliated montmorillonite nanoparticles.7. The hydrogel of claim 3 , wherein said nanoparticles are exfoliated montmorillonite nanoparticles.8. The hydrogel as in claim 1 , wherein said nanoparticles have a mean particle thickness of from about 0.8 to about 50 nm.9. The hydrogel as in claim 1 , wherein ...

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05-05-2016 дата публикации

FILTER AND CONTAINER HAVING MICROBICIDAL ACTIVITY

Номер: US20160121005A1
Принадлежит:

A sterilization filter includes a synthetic polymer film, the synthetic polymer film having a surface which has a plurality of first raised portions, a two-dimensional size of the plurality of first raised portions being more than 20 nm and less than 500 μm when viewed in a normal direction. The synthetic polymer film is arranged in a predetermined shape. In the arrangement of the predetermined shape, an inclination of a normal to the surface varies depending on a position over the surface, and an inclination of a normal to a surface opposite to the surface varies depending on a position over the opposite surface. At least part of the surface is capable of coming into contact with a gas or liquid. 1. A sterilization filter comprising a synthetic polymer film , the synthetic polymer film having a surface which has a plurality of first raised portions , a two-dimensional size of the plurality of first raised portions being more than 20 nm and less than 500 μm when viewed in a normal direction ,wherein the synthetic polymer film is arranged in a predetermined shape,in the arrangement of the predetermined shape, an inclination of a normal to the surface varies depending on a position over the surface, and an inclination of a normal to a surface opposite to the surface varies depending on a position over the opposite surface, andat least part of the surface is capable of coming into contact with a gas or liquid.2. The sterilization filter of wherein claim 1 , in the arrangement of the predetermined shape claim 1 , a cross section of the synthetic polymer film forms at least one ring.3. The sterilization filter of wherein claim 1 , in the arrangement of the predetermined shape claim 1 , a cross section of the synthetic polymer film forms a spiral.4. The sterilization filter of claim 1 , wherein the predetermined shape is a bellows-like shape.5. The sterilization filter of claim 1 , wherein the surface further has a plurality of spacer portions.6. The sterilization filter ...

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09-04-2020 дата публикации

Devices and Methods for Eluting and Concentrating Large DNA Molecules

Номер: US20200110009A1
Принадлежит:

This disclosure relates to mesofluidic devices and methods for eluting and concentrating a plurality of nucleic acid molecules. The mesofluidic device includes a device frame having a bottom surface upon which is defined a first reservoir and the second reservoir. The first reservoir includes a first electrode, and the second reservoir includes a second electrode. The first and second electrodes are configured for electrical connection. The mesofluidic device includes an elongated channel extending between the first reservoir and the second reservoir. The mesofluidic device includes a first slot having a first slot width. The first slot is configured to receive an insert. The first slot intersects the elongated channel. The mesofluidic device includes a second slot having a second slot width. The second slot is configured to receive a separation material having a first porosity. The second slot intersects the elongated channel. 1. A mesofluidic device for eluting and concentrating a plurality of nucleic acid molecules , the mesofluidic device comprising:a device frame having a bottom surface upon which is defined a first reservoir comprising a first electrode, a second reservoir comprising a second electrode, the first and second electrodes configured for electrical connection, and an elongated channel extending between the first reservoir and the second reservoir, the elongated channel having a channel width;a first slot having a first slot width, the first slot configured to receive an insert, wherein the first slot intersects the elongated channel; anda second slot having a second slot width, the second slot configured to receive a separation material having a first porosity wherein the second slot intersects the elongated channel,wherein the first slot width and the second slot with width are greater than the channel width.2. The mesofluidic device of claim 1 , wherein the plurality of nucleic acid molecules comprises deoxyribonucleic acid (DNA) molecules.3. The ...

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27-05-2021 дата публикации

Capillary Electrophoresis Device

Номер: US20210156821A1
Принадлежит:

An electrophoresis device has: a sample tray () on which there are placed a positive-electrode-side buffer solution container () containing a buffer solution and a phoresis medium container () containing a phoresis medium, and which is driven in a vertical direction and a horizontal direction; a thermostat oven unit () that holds a capillary array having a capillary head in which a plurality of capillaries are bundled in a single unit at one end thereof in a state where the capillary array being held in a state in which the capillary head protrudes downward, and that keeps the interior temperature constant; a solution-delivering mechanism () for delivering the phoresis medium in the phoresis medium container to the capillary array from the capillary head; and a power source for applying a voltage to both ends of the capillary array. Holes for insertion of the capillary head are provided in upper sections of the positive-electrode-side buffer solution container and the phoresis medium container. The thermostat oven unit is provided with a first lid member () that is positioned above the sample tray and seals the upper section of the positive-electrode-side buffer solution container while the phoresis medium is being delivered by the solution-delivering mechanism. 1. An electrophoresis device , comprising:a sample tray on which a positive-electrode-side buffer solution container containing a buffer solution and a phoresis medium container containing a phoresis medium are placed, and which is driven in a vertical direction and a horizontal direction;a thermostat oven unit that holds a capillary array having a capillary head in which a plurality of capillaries are bundled in a single unit at one end thereof in a state where the capillary head protrudes downward, and that keeps an interior temperature constant;a solution-delivering mechanism for delivering the phoresis medium in the phoresis medium container to the capillary array from the capillary head; anda power ...

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31-07-2014 дата публикации

Poly and Copoly(N-vinylamide)s and Their Use In Capillary Electrophoresis

Номер: US20140209461A1
Автор: LAU Aldrich N.K.
Принадлежит: APPLIED BIOSYSTEMS, LLC

The invention relates generally to polymers and copolymers comprising N-vinylamide-type monomers, their preparation, and compositions, such as electrophoresis separation media, containing the same; to supports, such as capillaries, containing these polymers; and methods for separating a mixture of biomolecules, especially polynucleotides, using capillary electrophoresis. Separation media comprising such polymers yield advantageous performance in the analysis and separation of biomolecules by capillary electrophoresis. 1. A composition comprising a buffer , a denaturant , and a uncrosslinked water soluble polymer having the form poly(MM) , or a salt thereof , comprising one or more monomers of type M , wherein:(a) y is zero;{'sub': '1', '(b) each monomer in the polymer is of type M;'}{'sub': '1', '(c) x is an integer ranging from 1 to 5 and represents the number of monomer subtypes of type Mthat are present in the polymer; and'}{'sub': '1', '(d) each monomer subtype of type Min the polymer is fromN-vinylhexamide, N-vinylheptamide, N-vinyloctamide, N-vinylnonamide, N-vinyldecamide, N-vinylformamide, N-methyl-N-vinylformamide, N-vinylacetamide, N-methyl-N-vinylacetamide, N-methyl-N-vinylpropamide, N-methyl-N-vinylbutamide, N-methyl-N-vinylpentamide, N-methyl-N-vinylhexamide, N-methyl-N-vinylheptamide, N-methyl-N-vinyloctamide, N-methyl-N-vinylnonamide, N-methyl-N-vinyldecamide, N-ethyl-N-vinylformamide, N-ethyl-N-vinylacetamide, N-ethyl-N-vinylpropamide, N-ethyl-N-vinylbutamide, N-ethyl-N-vinylpentamide, N-ethyl-N-vinylhexamide, N-ethyl-N-vinylheptamide, N-ethyl-N-vinyloctamide, N-ethyl-N-vinylnonamide, N-ethyl-N-vinyldecamide, N-n-propyl-N-vinylformamide, N-n-propyl-N-vinylacetamide, N-n-propyl-N-vinylpropamide, N-n-propyl-N-vinylbutamide, N-n-propyl-N-vinylpentamide, N-n-propyl-N-vinylhexamide, N-n-propyl-N-vinylheptamide, N-n-propyl-N-vinyloctamide, N-n-propyl-N-vinylnonamide, N-n-propyl-N-vinyldecamide, N-iso-propyl-N-vinylformamide, N-iso-propyl-N-vinylacetamide, ...

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21-05-2015 дата публикации

WATER-SOLUBLE, UV-ABSORBING AND/OR FLOURESCENT COMPONENTS WITH VERY HIGH ISOELECTRIC POINTS

Номер: US20150136603A1
Принадлежит:

Water-soluble, UV-absorbing and/or fluorescing compound having an isoelectric point greater than 10, can be obtained using molecules chosen from the group of molecules consisting of those containing at least one secondary alcohol OH group with a pKvalue greater than 10 and at least one amino group with a pKvalue smaller than 5, those containing at least one primary alcohol OH group and at least one secondary alcohol OH group with respective pKvalues greater than 10 and at least one amino el group with a pKvalue smaller than 5 or a quaternary ammonium group, and those containing at least two secondary alcohol OH groups with respective pKvalues greater than 10 and at least one amino group with a pKvalue smaller than 5 or a quaternary ammonium group; and having at least one UV absorbing group or at least one fluorescing group or a combination thereof. The invention includes methods for producing such compounds. 111.-. (canceled)12. A method of establishing the course of a pH gradient in an isoelectric focusing separation compartment , comprising ,providing to the separation compartment:an analyte anda water-soluble, UV-absorbing and/or fluorescing compound having an isoelectric point greater than 10, wherein the water-soluble, UV-absorbing and/or fluorescing compound comprises:{'sub': a', 'b, 'a compound having at least one secondary alcohol group with a pKvalue greater than 10 and at least one amino group with a pKvalue smaller than 5,'}{'sub': a', 'b, 'a compound having at least one primary alcohol group and at least one secondary alcohol group with respective pKvalues greater than 10 and at least one amino group with a pKvalue smaller than 5 or a quaternary ammonium group, or'}{'sub': a', 'b, 'a compound having at least two secondary alcohol groups with respective pKvalues greater than 10 and at least one amino group with a pKvalue smaller than 5 or a quaternary ammonium group;'}wherein the compound has at least one UV absorbing group, at least one fluorescing group ...

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21-05-2015 дата публикации

ELECTROPHORESIS TRAY AND A METHOD OF RUNNING AN ELECTROPHORESIS EXPERIMENT

Номер: US20150136605A1
Принадлежит: GE HEALTHCARE BIO-SCIENCES AB

Electrophoresis tray arranged to support an electrophoresis cassette for miming electrophoresis experiments, the electrophoresis cassette comprising a gel member in a housing with a front and a back face, wherein the tray comprises a cassette support surface for supporting at least the separation zone of the electrophoresis cassette during electrophoresis, and wherein the cassette support surface is flanked by a pair of buffer pad holders each one arranged to hold a buffer pad in a mating position with respect to buffer connection sections at the back face of the electrophoresis cassette. 1. Electrophoresis tray arranged to support an electrophoresis cassette for running electrophoresis experiments , the electrophoresis cassette comprising a gel member in a housing with a front and a back face ,wherein the tray comprises a cassette support surface for supporting at least the separation zone of the electrophoresis cassette during electrophoresis, andwherein the cassette support surface is flanked by a pair of buffer pad holders each one arranged to hold a buffer pad in a mating position with respect to buffer connection sections at the back face of the electrophoresis cassette.2. Electrophoresis tray according to comprising a heat transfer unit connected to the cassette support surface to control the temperature of the electrophoresis cassette during electrophoresis by heat transfer contact with a section of the back surface of the electrophoresis cassette.3. Electrophoresis tray according to formed to provide a biased mating of the buffer pads and the buffer connection sections at the back face of the electrophoresis cassette.4. Electrophoresis tray according to wherein the buffer pads are spring loaded in the biasing direction.5. Electrophoresis tray according to claim 1 , wherein the buffer pads are comprised of a resilient material.6. Electrophoresis tray according to claim 1 , wherein each buffer pad holder comprises a buffer cup with a compartment for holding a ...

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02-05-2019 дата публикации

WATER CONTROL IN DIELECTRIC FLUID LAYERS

Номер: US20190126203A1
Принадлежит:

A system and method are described for regulating water concentration in a dielectric fluid. The system can comprise electrodes capable of applying an electric field across the dielectric fluid, thereby causing charge carriers and water included in the charge carriers to move toward a respective electrode. The system further includes a trap positioned near the electrodes and comprising a water absorbing material capable of trapping water, thereby reducing the concentration of water in the dielectric fluid. It is also possible to measure properties of the dielectric fluid and use such measurements to control the water-trapping process. 1. A method for regulating water concentration in a dielectric fluid , the method comprising:applying an electric field via electrodes positioned across the dielectric fluid, thereby causing charge carriers and water included in the charge carriers to move toward a respective electrode; andtrapping water by using a water absorbing material positioned near the respective electrode, thereby reducing the concentration of water in the dielectric fluid.2. The method of claim 1 , wherein the charge carriers comprise inverse micelles claim 1 , non-micellar macromolecular structures claim 1 , and/or aggregates.3. The method of claim 1 , wherein the water absorbing material comprises zeolite.4. The method of claim 1 , further comprising:measuring an electrical property of the dielectric fluid; anddetermining, based on the measured electrical property of the dielectric fluid, an ionic conductivity and/or a concentration of charge carriers of the dielectric fluid.5. The method of claim 4 , wherein measuring the electrical property of the dielectric fluid further comprises measuring an electric current in the dielectric fluid corresponding to a known voltage source.6. The method of claim 1 , further comprising:measuring an optical property of the dielectric fluid; anddetermining the water concentration based on the measured optical property.7. The ...

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01-09-2022 дата публикации

NANOPORE DEVICE AND METHOD OF MANUFACTURING SAME

Номер: US20220276219A1
Принадлежит: PALOGEN, INC.

A 3D nanopore device for characterizing biopolymer molecules includes a first selecting layer having a first axis of selection. The device also includes a second selecting layer disposed adjacent the first selecting layer and having a second axis of selection orthogonal to the first axis of selection. The device further includes an third electrode layer disposed adjacent the second selecting layer, such that the first selecting layer, the second selecting layer, and the third electrode layer form a stack of layers along a Z axis and define a plurality of nanopore pillars. 1. A method of detecting a charged particle using a 3D nanopore device having top , middle , and bottom chambers , and a 3D nanopore array disposed in the middle chamber such that the top and bottom chambers are fluidly coupled by a plurality of nanopores in the 3D nanopore array , the method comprising:adding electrolyte solution including the charged particle to the top, middle, and bottom chambers;placing top and bottom electrodes in the top and bottom chambers respectively;applying an electrophoretic bias between the top and bottom electrodes;applying first and second selection biases to first and second selection electrodes in the 3D nanopore device to select one or more nanopores of the plurality of nanopores through which the charged particle will be directed;applying a rate control bias to a rate control electrode in the 3D nanopore device to modulate a translocation rate of the charged particle through the one or more nanopores;applying a sensing bias to a sensing electrode in the 3D nanopore device; anddetecting a change in a current in the sensing electrode.2. The method of claim 1 , wherein the current is an electrode current.3. The method of claim 1 , wherein the current is a tunneling current.4. A 3D nanopore device for characterizing biopolymer molecules claim 1 , comprising:a first selecting layer having a first axis of selection;a second selecting layer disposed adjacent the first ...

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19-05-2016 дата публикации

Apparatus for Analyzing Ion Kinetics in Dielectrics

Номер: US20160139077A1
Принадлежит:

An apparatus for analyzing ion kinetics in a dielectric probe structure includes an ion reservoir abutting the dielectric probe structure and configured to supply mobile ions to the dielectric probe structure, a capacitor structure configured to generate an electric field in the dielectric probe structure along a vertical direction, and an electrode structure configured to generate an electrophoretic force on mobile ions in the dielectric probe structure along a lateral direction. A method for analyzing ion kinetics in the dielectric probe structure of the apparatus is also provided. 1. An apparatus for analyzing ion kinetics in a dielectric probe structure , comprising:an ion reservoir abutting the dielectric probe structure and configured to supply mobile ions to the dielectric probe structure;a capacitor structure configured to generate an electric field in the dielectric probe structure along a vertical direction; andan electrode structure configured to generate an electrophoretic force on mobile ions in the dielectric probe structure along a lateral direction.2. The apparatus of claim 1 , further comprising a processing unit configured to determine an electric characteristic of the capacitor structure based on the presence of mobile ions in the dielectric probe structure.3. The apparatus of claim 1 , wherein the capacitor structure comprises a metal insulator semiconductor field effect transistor structure.4. The apparatus of claim 1 , wherein the capacitor structure comprises:a semiconductor body, the dielectric probe structure being disposed on the semiconductor body; anda capacitor electrode on the dielectric probe structure.5. The apparatus of claim 4 , wherein the capacitor electrode comprises at least two capacitor electrode parts each being separated in the lateral direction.6. The apparatus of claim 4 , wherein the capacitor structure further comprises a dielectric base structure between the semiconductor body and the dielectric probe structure.7. The ...

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03-06-2021 дата публикации

ELECTROPHORESIS GEL CASSETTE

Номер: US20210164936A1
Автор: Lee Kuan-Lin
Принадлежит: SMOBIO TECHNOLOGY, INC.

An electrophoresis gel cassette is provided, including a first plate, a second plate, a plurality of spacers and at least one first protrusion, wherein the first plate and the second plate respectively includes a top side, a bottom side, a left side, a right side, a first surface and a second surface, the second plate is substantially parallel to the first plate so that the first surface of the first plate and the first surface of the second plate are parallel to each other, and the plurality of spacers are disposed on a left portion and a right portion of the first surface of the first plate or the second plate, and the first plate, the second plate, and the plurality of spacers are assembled together to form a gel accommodating space, and the at least one first protrusion is disposed at an appropriate position on a bottom portion of the first surface of the first plate or the second plate for connecting the first plate and the second plate so as to maintain the electrophoresis gel accommodating space. 1. An electrophoresis gel cassette , comprising:a first plate, a second plate, and a plurality of spacers, wherein the first plate and the second plate respectively comprises a top side, a bottom side, a left side, a right side, a first surface and a second surface, when the second plate is disposed substantially parallel to the first plate, the first surface of the first plate and the first surface of the second plate are parallel to each other, and the plurality of spacers are disposed on a left portion and a right portion of the first surface of the first plate or the second plate, in which a gel accommodating space is formed; andat least one first protrusion is disposed at an appropriate position on a bottom portion of the first surface of the first plate or the second plate for connecting the first plate and the second plate so as to maintain the electrophoresis gel accommodating space.2. The electrophoresis gel cassette of claim 1 , wherein the appropriate ...

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03-06-2021 дата публикации

CAPILLARY CARTRIDGE AND ELECTROPHORESIS APPARATUS

Номер: US20210164937A1
Принадлежит:

A capillary cartridge achieves both improvement of attachability and improvement of heat dissipation performance for realizing short-time analysis. A heat dissipation body is provided between a capillary having a detection unit provided in a part thereof and a plate-like support body that supports the capillary, and temperature increase inside the capillary is suppressed by the heat dissipation body, and thereby, electrophoresis can be performed under a high voltage application condition where the amount of heat increases and analysis time is reduced. In addition, it is possible to redress complexity of an operation by reducing a fixing place at the time of attachment to only a detection unit and an electrode holder by using an integration structure in which the capillary, the supporting body, and the heat radiating body are integrated. 1. An electrophoresis apparatus comprising: a capillary array having a capillary, a capillary head that is provided one end of the capillary, an electrode that is provided in another end of the capillary, and a detection unit that is provided in a part of the capillary; and', 'a support body that supports the capillary; and, 'a capillary cartridge havinga thermostatic chamber that holds the capillary at a predetermined temperature,wherein one of the thermostatic chamber and the capillary cartridge has a positioning pin that positions the capillary cartridge in the thermostatic chamber,wherein the other of the thermostatic chamber and the capillary cartridge has a positioning hole in which the positioning pin is inserted,wherein, by inserting the positioning pin to the positioning hole, the capillary cartridge is positioned in the thermostatic chamber, andwherein a mounting surface of the capillary cartridge of the thermostatic chamber has a clip comprising an elastic body and a strut to fix the elastic body, and the clip holds the detection unit by the reaction force of the elastic body.2. The electrophoresis apparatus according to ...

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28-05-2015 дата публикации

SOLID-LIQUID SEPARATION METHOD

Номер: US20150144491A1
Автор: Noguchi Hiroshi
Принадлежит: MEIDENSHA CORPORATION

To separate solid matter contained in a liquid phase in a membrane separation tank a pH of the liquid phase is adjusted to make a surface charge of the solid matter electrically homopolar with a surface charge of a ceramic flat membrane to separate the solid matter from the liquid phase. Alternatively, by adding, to the liquid phase, a charge adjusting agent having a surface charge electrically homopolar to a surface charge of the ceramic flat membrane and electrically heteropolar to a surface charge of the solid matter, the surface of the solid matter is charged to have an apparent surface charge electrically homopolar to the surface charge of the inorganic membrane 18-. (canceled)9. A solid-liquid separating method for solid-liquid separation of solid matter contained in a liquid phase with an inorganic membrane , the solid-liquid separating method comprising:a step of measuring isoelectric points of the solid matter and the inorganic membrane in advance; anda step of adjusting a pH of the liquid phase in accordance with the isoelectric points at a time of the solid-liquid separation,in the step of adjusting the pH, the pH of the liquid phase being adjusted to a lower pH value lower than the isoelectric point of the solid matter and the isoelectric point of the inorganic membrane or a higher pH value higher than the isoelectric point of the solid matter and the isoelectric point of the inorganic membrane.10. The solid-liquid separating method as recited in claim 9 , wherein the solid matter is formed by adding a charge adjusting agent claim 9 , and the charge adjusting agent has a surface charge electrically heteropolar to a surface charge of the solid matter contained in the liquid phase before addition of the charge adjusting agent.11. The solid-liquid separating method as recited in claim 10 , wherein the solid matter formed by addition of the charge adjusting agent has a surface charge electrically homopolar to a surface charge of another solid matter formed ...

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08-09-2022 дата публикации

INTRAGEL WELL SAMPLE LOADING SYSTEM

Номер: US20220281913A1
Принадлежит:

The present invention provides a protein separation micro el having a first plate having a first opening; a second plate; a gel substrate formed between the first plate and the second plate; and a plurality of wells positioned at a first non-zero distance from a first edge of the gel and at a second non-zero distance from a second edge of the gel; wherein the first and second edge of the gel are opposite edges of the gel. 1. A protein separation microgel comprising:a first plate having a first opening;a second plate;a gel substrate formed between the first plate and the second plate; anda plurality of wells positioned at a first non-zero distance from a first edge of the gel and at a second non-zero distance from a second edge of the gel;wherein the first and second edge of the gel are opposite edges of the gel.2. The protein separation microgel of claim 1 , wherein the plurality of wells are positioned collinearly.3. The protein separation microgel of claim 1 , wherein the gel substrate comprises polyacrylamide.4. The protein separation microgel of claim 1 , wherein the second plate comprises a second opening claim 1 , wherein the opening of the second plate is aligned with the opening of the first plate.5. The protein separation microgel of claim 1 , wherein the plurality of collinear wells are formed by a well-forming insert comprising a plurality of teeth extending through the gel substrate within the first opening.6. The protein separation microgel of claim 5 , wherein the plurality of teeth extend through the gel at an orthogonal angle to the plane of the gel substrate.7. The protein separation microgel of claim 3 , wherein the polyacrylamide gel comprises between about 3% acrylamide and about 20% acrylamide by weight.8. The protein separation microgel of claim 3 , wherein the polyacrylamide gel comprises 5% acrylamide claim 3 , 10% acrylamide claim 3 , 12.5% acrylamide claim 3 , 15% acrylamide claim 3 , or 20% acrylamide by weight.9. The protein separation ...

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26-05-2016 дата публикации

SYSTEM AND METHOD FOR THE CONCENTRATION OF A SLURRY

Номер: US20160144319A1
Принадлежит: Omya International AG

The present invention relates to a system and a method for the concentration of slurry, especially mineral containing slurry. The invention provides a system comprising an electrophoresis unit and a separation unit, where the separation unit comprises a recipient, preferably of half cylindrical form, with adjusted flanks for separating the solid material or cake from the rotating anodes and a sliding carriage suitable for closing the recipient and stripping resting solid material from the flanks into the recipient before the solid material or cake is pressed out of the recipient by a piston. 1. A separation unit as part of a device for the concentration of slurry , comprising a recipient and a piston adapted to the recipient form , wherein the recipient has shoulders dimensioned to act as scraping flanges for taking off the cake material from an anode , the piston is dimensioned for pressing the cake material out of the recipient , the separation unit has a sliding carriage with a cover for closing the recipient and collecting residual cake material from the shoulders into the recipient while closing it , and the recipient has a half cylindrical form.2. The separation unit according to claim 1 , wherein the separation unit is made of poly-tetra-fluoroethylene.3. The separation unit according to claim 1 , wherein the piston is driven pneumatically.4. The separation unit according to claim 1 , wherein the recipient has a half cylindrical or half rectangular form.5. The separation unit according to claim 1 , wherein the device is for the concentration of a slurry comprising a mineral claim 1 , with a supporting structure including modules received therein claim 1 , the modules comprising a) an electrophoresis cell with at least one electrically connected cathode claim 1 , and at least one electrically connected claim 1 , rotatable anode disc claim 1 , and b) the separation unit. This is a Divisional of U.S. application Ser. No. 13/641,755, filed Dec. 21, 2012, which is ...

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