KIND OF MUTATED PROTEINS A WITH HIGH ALKALI RESISTANCE FEATURE AND APPLICATION THEREOF

A series of protein A mutants having high alkali resistance, and methods of using the protein A mutants are provided. The protein A mutants have a high binding affinity for regions of immunoglobulin proteins other than the complementarity determining regions. The protein A mutants can be coupled to a solid support for immunoglobulin isolation, or conjugated to a label for immunoglobulin detection. This series of protein A mutants has high chemical stability under alkaline conditions of pH 13-14, and can also be used as chromatography ligands for purification procedures that use alkaline solutions under harsh conditions, such as Clean-In-Place (CIP). Also provided are methods of immunoglobulin separation and purification, and alkali regeneration of affinity chromatography medium that uses protein A as a ligand. 116-. (canceled)17. A protein A mutant comprising the amino acid sequence of SEQ ID NO:1 or SEQ ID NO: 2 , or an amino acid sequence that is at least 99% identical to amino acid residues 7 to 54 of SEQ ID NO: 1 or amino acid residues 7 to 54 of SEQ ID NO: 2.18. The protein A mutant of claim 17 , wherein the protein A mutant is stable under alkaline conditions of pH 13-14.19. A multimer of a protein A mutant comprising at least two protein A mutants of claim 17 , wherein the at least two protein A mutants comprise the same amino acid sequence or different amino acid sequences.20. The multimer of claim 19 , wherein the multimer is a dimer claim 19 , trimer claim 19 , or tetramer comprising two claim 19 , three claim 19 , or four protein A mutants claim 19 , respectively.21. The multimer of claim 19 , wherein the one or more protein A mutants are linked together via one or more linker units comprising 4 to 10 amino acids.22. The multimer of claim 21 , wherein the linker unit comprises the amino acid sequence ADGK (SEQ ID NO: 56).23. The multimer of claim 19 , further comprising six histidine residues fused to the N-terminal end of the multimer.24. The multimer of ...

METHOD AND APPARATUS FOR FORWARDING MULTICAST TRAFFIC

Embodiments of the present invention provide a method and an apparatus for forwarding multicast traffic. The method includes: receiving a third multicast join message; in response to the third multicast join message, sending a first multicast join message to a first upstream router, and establishing an active path; in response to the third multicast join message, sending a second multicast join message to a second upstream router, and establishing a standby path; and sending multicast traffic to the multicast receiver through the active path, where the standby path does not forward the multicast traffic. In the foregoing embodiments, the standby path that does not forward the multicast traffic is established beforehand, so that when a fault occurs in the active path, the standby path established beforehand can be used to forward the multicast traffic. 1. A method for forwarding multicast traffic , the method comprising:receiving a third multicast join message;in response to the third multicast join message, sending a first multicast join message to a first upstream router, and establishing an active path;in response to the third multicast join message, sending a second multicast join message to a second upstream router, and establishing a standby path; andsending multicast traffic to a multicast receiver through the active path, wherein the standby path does not forward the multicast traffic.2. The method according to claim 1 , further comprising: forwarding the multicast traffic through the standby path when a fault occurs in the active path.3. The method according to claim 2 , wherein the second multicast join message comprises active path information used for identifying primary inbound interface information of the active path protected by the standby path claim 2 , and the forwarding the multicast traffic through the standby path when a fault occurs in the active path comprises: when a fault occurs in the active path claim 2 , determining claim 2 , by a router ...

APPARATUS AND METHOD FOR SEMI-AUTOMATED PARALLEL SYNTHESIS OF PEPTIDES

An apparatus is provided for the semi-automated parallel synthesis of multiple peptides. The apparatus includes an array of nozzles, each positioned above or adjacent a separate reaction container, two or more liquid reservoirs, each reservoir coupled to the liquid dispenser(s), and base chamber(s) connected to the reaction containers for removing liquid there from. The addition and removal of liquids may be controlled by programmable electromagnetic valves. The apparatus may also be used for other parallel solid phase reactions. A method is also provided for synthesizing multiple polypeptides or other macromolecules, for example by using the above apparatus, wherein multiple common steps are performed automatically while reactants are added manually. 1. An apparatus for semi-automated parallel synthesis of multiple macromolecules , wherein the apparatus comprises at least two liquid reservoirs each in liquid communication with at least one multi-channel liquid dispenser having an array of n nozzles , an array of n reaction containers containing solid substrates , wherein each nozzle is positioned above or adjacent a different reaction container , and at least one base chamber connected to the array of reaction containers for receiving waste from the n reaction containers , wherein n is an integer from two to about 100.2. The apparatus according to claim 1 , wherein the solid substrates are polymer resins.3. The apparatus according to claim 1 , wherein the macromolecules are selected from the group consisting of peptides claim 1 , DNA claim 1 , RNA claim 1 , and modified oligonucleotides.4. The apparatus according to claim 1 , wherein each of the at least two liquid reservoirs contains a reagent common to the synthesis of the multiple macromolecules.5. The apparatus according to claim 4 , wherein the reagent is selected from the group consisting of a washing liquid and a deprotecting liquid.6. The apparatus according to claim 1 , wherein each reaction container ...

TUNNEL FAULT DETECTION METHOD AND TRAFFIC ENGINEERING NODE

The embodiments of the present invention provide a tunnel fault detection method and a traffic engineering (TE) node. The method includes: receiving, by a traffic engineering TE node, a link fault report packet; determining, by the node, a faulty link in which a fault occurs according to the link fault report packet; detecting, by the node, whether one or more tunnels which use the node as an ingress node and pass through the faulty link exist; and identifying the tunnels as faulty tunnels if the one or more tunnels which use the node as the ingress node and pass through the faulty link exist. In the embodiments of the present invention, the TE node can detect all faulty tunnels at a time, and it is not necessary to wait until the fault notification based on each tunnel is received and then the faulty tunnel is identified one by one. 1. A tunnel fault detection method , comprising:receiving, by a traffic engineering TE node, a link fault report packet;determining, by the node, a faulty link in which a fault occurs according to the link fault report packet;detecting, by the node, whether one or more tunnels which use the node as an ingress node and pass through the faulty link exist; andif the one or more tunnels which use the node as the ingress node and pass through the faulty link exist, identifying the tunnels as faulty tunnels.2. The method according to claim 1 , wherein: the detecting claim 1 , by the node claim 1 , whether one or more tunnels which use the node as the ingress node and pass through the faulty link exist claim 1 , comprises:according to link information of links respectively passed through by multiple tunnels that use the node as the ingress node and according to link information of the faulty link, detecting, by the node, whether the one or more tunnels which use the node as the ingress node and pass through the faulty link exist.3. The method according to claim 1 , wherein: after identifying the tunnels as faulty tunnels as faulty tunnels ...

FREE-STANDING NON-FOULING POLYMERS, THEIR COMPOSITIONS, AND RELATED MONOMERS

Free-standing non-fouling polymers and polymeric compositions, monomers and macromonomers for making the polymers and polymeric compositions, objects made from the polymers and polymeric compositions, and methods for making and using the polymers and polymeric compositions. 119-. (canceled)2122-. (canceled)23. A polymer of having a fibrinogen binding level of less than 30 ng/cmin a fibrinogen binding assay (polymer surface is incubated at 37° C. for 90 minutes with a 1.0 mg/mL fibrinogen solution in 0.15 M phosphate buffered saline at pH 7.4).24. A polymer of having a tensile or compressive strength greater than 0.5 MPa.25. A polymer of having a fibrinogen binding level of less than 30 ng/cmin a fibrinogen binding assay (polymer surface is incubated at 37° C. for 90 minutes with a 1.0 mg/mL fibrinogen solution in 0.15 M phosphate buffered saline at pH 7.4) and a tensile or compressive strengths greater than 0.5 MPa.26. A polymer of claim 20 , wherein the polymer is crosslinked.27. A composite claim 20 , comprising a polymer of and a second polymer.28. The composite of claim 27 , wherein the second polymer is selected from the group consisting of polyesters claim 27 , polycarbonates claim 27 , polyurethanes claim 27 , polyureas claim 27 , polysulfides claim 27 , polysulfones claim 27 , polyimides claim 27 , polyepoxies claim 27 , aromatic polyesters claim 27 , cellulosics claim 27 , fluoropolymers claim 27 , polyacrylics claim 27 , polyamides claim 27 , polyanhydrides claim 27 , polyethers claim 27 , vinyl polymers claim 27 , phenolics claim 27 , elastomers claim 27 , and other addition polymers.29. The composite of further comprising a strength additive.30. The composite of further comprising a strength additive selected from the groups consisting of fiber claim 27 , clays claim 27 , nanotubes claim 27 , and other inorganic objects.31. A polymer of formed into an object by a method selected from the group consisting of injection molding claim 20 , blow molding claim ...

PROTEIN A MUTANTS HAVING HIGH ALKALI RESISTANCE AND METHODS OF USE THEREOF

A series of protein A mutants having high alkali resistance, and methods of using the protein A mutants are provided. The protein A mutants have a high binding affinity for regions of immunoglobulin proteins other than the complementarity determining regions. The protein A mutants can be coupled to a solid support for immunoglobulin isolation, or conjugated to a label for immunoglobulin detection. This series of protein A mutants have high chemical stability under alkaline conditions of pH 13-14, and can also be used as chromatography ligands for purification procedures that use alkaline solutions under harsh conditions, such as Clean-In-Place (CIP). Also provided are methods of immunoglobublin separation and purification, and alkali regeneration of affinity chromatography medium that uses protein A as a ligand. 116-. (canceled)17. A multimer of a recombinantly expressed mutant protein A molecule comprising at least two recombinant expressed mutant protein A molecules for isolating , purifying , or detecting an immunoglobulin , the mutant protein A molecule comprising the amino acid sequence of SEQ ID NO:1 , the amino acid sequence of SEQ ID NO:2 , the amino acid sequence of residues 7 to 54 of SEQ ID NO:1 , or the amino acid sequence of residues 7 to 54 of SEQ ID NO:2 , wherein the recombinantly expressed mutant protein A molecule comprises an amino acid substitution at an amino acid position selected from the group consisting of amino acid position 7 , 10 , 33 , 44 , 46 , 47 , 48 , and 50 corresponding to the wild type protein A sequence.18. The multimer of claim 17 , wherein the mutant protein A molecule is stable under alkaline conditions of pH 13-14.19. The multimer of claim 17 , wherein the at least two recombinantly expressed mutant protein A molecules comprise the same amino acid sequence or different amino acid sequences.20. The multimer of claim 19 , wherein the multimer is a dimer claim 19 , trimer claim 19 , or tetramer comprising two claim 19 , three ...

GEL ELECTROPHORESIS DEVICE FOR LOADING LARGE SAMPLE VOLUMES

A device for gel electrophoresis is provided having larger wells for loading an increased sample volume with improved gel resolution. The device includes a gel cassette having a front plate and a back plate, wherein at least one plate has a stepped inner surface to create a wider opening at the top of the gel cassette, a gel matrix, and a comb with teeth having a thickness substantially equal to the spacing at the top opening of the gel cassette. Using this device, the sample volume in each well can be increased and the sample height in each well can be significantly reduced, as compared to loading the same volume in the wells of a standard gel cassette. 1. A device for performing gel electrophoresis , the device comprising:(i) a gel cassette comprising a front plate and a back plate, the front plate and the back plate each having an inner surface, a top edge, a bottom edge and two side edges, the front and back plates being fastened together along at least the two side edges, such that the inner surfaces are face-to-face with a spacing there between forming a gel chamber, wherein the inner surface of at least one of the front and back plates has a stepped configuration such that the at least one plate has a top portion and a bottom portion, wherein the top portion has a thickness at least 0.05 mm thinner than the bottom portion, and the spacing between the plates is greater at the top portion than the spacing between the plates at the bottom portion;(ii) a gel matrix held within the gel chamber; and(iii) a comb inserted in the spacing at the top portion of the plates, the comb having a spine and at least one tooth extending from the spine downwardly into the gel matrix to form wells in the gel matrix, the at least one tooth having a thickness substantially equal to the spacing between the top portions of the plates, such that upon removal of the comb for performing the gel electrophoresis, the wells are bounded on opposite sides by the top portions of the plates.2. ...

INCLINED MAGNETIC HOLDER

An inclined magnetic holder is disclosed, comprising a magnetic base and a centrifuge tube support plate. The centrifuge tube support plate is provided with centrifuge tube support holes. The magnetic base comprises a first bottom plate, a fixing plate provided on the first bottom plate, and two first-side support plates respectively provided at two sides of the fixing plate. Respective top portions of the two first-side support plates are provided with a position-locating slot. A bottom surface of the position-locating slot is configured with an inclined angle. Two ends of the centrifuge tube support plate are respectively provided with a position-locating protruding block for fitting and assembling into the position-locating slot. The centrifuge tube support holes are evenly and linearly distributed on the centrifuge tube support plate. An elastic circular engagement component for holding a centrifuge tube is provided inside the centrifuge tube support holes. A block magnet is fixed to the fixing plate at a location below and corresponding to each of the centrifuge tube support holes. The block magnets below each of the centrifuge tube support holes correspond to each of the centrifuge tubes. A north pole or south pole surface of the block magnet faces the centrifuge tube and is parallel to an axis of the centrifuge tube. 1121111211131261362621622451255. An inclined magnetic holder device , comprising a magnetic base () and a centrifugal tube supporting plate () having centrifugal tube supporting holes thereon , wherein the magnetic base () comprises a first baseplate (-) , a fixing plate (-) disposed on the first baseplate (-) and two first side supporting plates (-) located at two sides of the fixing plate (-); positioning grooves () are respectively provided on the top of the two first side supporting plates (-); bottom surfaces (-) of the positioning grooves () are disposed inclinedly; positioning bumps (-) for engaging in the positioning grooves () are ...

METHOD AND APPARATUS FOR DETERMINING NEXT HOP AND ADVERTISING ROUTING INFORMATION

Embodiments of the present application disclose a method and an apparatus for determining a next hop and advertising routing information, which relate to the field of communications and are used for reducing operation complexity of a forwarding device and reducing network maintenance workload. The method for determining the next hop provided by the embodiments of the present application includes: receiving, by a control device, first routing information sent by a first forwarding device, where the first routing information includes an identifier of the first forwarding device; determining a first routing path according to the identifier of the first forwarding device, an identifier of a second forwarding device and a routing path group; and determining a next hop in second routing information for the second forwarding device according to the first routing path, where the second forwarding device is a receiver of the second routing information advertised by the control device. 1. A method for determining a next hop , comprising:receiving, by a control device, first routing information sent by a first forwarding device, wherein the first routing information comprises an identifier of the first forwarding device;determining a first routing path according to the identifier of the first forwarding device, an identifier of a second forwarding device and a routing path group; anddetermining a next hop in second routing information for the second forwarding device according to the first routing path, wherein the second forwarding device is a receiver of the second routing information advertised by the control device.2. The method according to claim 1 , wherein the determining the next hop in the second routing information for the second forwarding device according to the first routing path claim 1 , comprises:determining an identifier of an adjacent node before the second forwarding device on the first routing path as the next hop in the second routing information for the ...

METHOD, APPARATUS AND SYSTEM FOR CONTROLLING ROUTING INFORMATION ADVERTISING

The application disclose a method, an apparatus and a system for controlling routing information advertising, which relates to the field of communications and is used for reducing the configuration complexity and reinforcing the operability. The method includes: receiving, by a control device, first routing information sent by a first forwarding device; wherein the first routing information includes an identifier of the first forwarding device; determining a first routing path according to the identifier of the first forwarding device, an identifier of a second forwarding device and a routing path group; and determining an advertising range of second routing information for the second forwarding device according to the first routing path; for enabling the second forwarding device to advertise the second routing information according to the advertising range of the second routing information. 1. A method for controlling routing information advertising , comprising:receiving, by a control device, first routing information sent by a first forwarding device, wherein the first routing information comprises an identifier of the first forwarding device;determining a first routing path according to the identifier of the first forwarding device, an identifier of a second forwarding device and a routing path group; anddetermining an advertising range of second routing information for the second forwarding device according to the first routing path;wherein the second routing information is routing information generated by the control device according to the first routing information, the second forwarding device is a receiver of the second routing information advertised by the control device, and the advertising range of the second routing information comprises an identifier of an external neighbor of the second forwarding device, for enabling the second forwarding device to advertise the second routing information according to the advertising range of the second routing ...

MULTIPATH TRANSMISSION BASED PACKET TRAFFIC CONTROL METHOD AND APPARATUS

Embodiments of the present application disclose a multipath transmission based packet traffic control method, including: monitoring egress traffic of a data packet set sent by a source node; determining, according to a preconfigured mapping relationship between traffic ranges and forwarding paths, a target forwarding path set corresponding to the egress traffic of the data packet set, and a weight parameter of the egress traffic borne on each target forwarding path in the target forwarding path set; and distributing the data packet set according to the weight parameter, and transferring the data packet set to a destination node by the target forwarding path set. The embodiments of the present application further disclose a traffic control apparatus. By using the present application, centralized policing and classification can be performed on user traffic in a multipath transmission scenario. 1. A multipath transmission based packet traffic control method , comprising:monitoring egress traffic of a data packet set sent by a source node;determining, according to a preconfigured mapping relationship between traffic ranges and forwarding paths, a target forwarding path set corresponding to the egress traffic of the data packet set, and a weight parameter of the egress traffic borne on each target forwarding path in the target forwarding path set; anddistributing the data packet set according to the weight parameter, and transferring the data packet set to a destination node by the target forwarding path set.2. The method according to claim 1 , wherein the monitoring egress traffic of a data packet set sent by a source node comprises:sending, to a concentration device, statistical information of the data packet set sent by the source node; andacquiring the egress traffic of the data packet set, wherein the egress traffic of the data packet set is calculated by the concentration device according to the statistical information.3. The method according to claim 1 , wherein ...

BUILDING BLOCK AND BUILDING BLOCK KIT

A building block includes at least one of a plug portion and a socket portion. The plug portion has two cantilevered tongues deflectable toward each other. Each cantilevered tongue includes a first engaging portion. The socket portion defines a chamber and has two second engaging portions. The plug portion and the socket portion are configured in such a way that the plug portion of one building block is insertable into the chamber of the socket portion of another building block, and the two cantilevered tongues of the building block deflect toward each other after insertion of the plug portion of the building block into the chamber of the socket portion of the other building block, which allows the first engaging portions of the building block to be engaged with the second engaging portions of the other building block, thereby connecting the two building blocks together. 1. A female building block connectable to a male building block , the male building block comprising a plug portion that comprises two cantilevered tongues spaced apart from and deflectable toward each other , each of the two cantilevered tongues comprising a first engaging portion at an end thereof , the female building block comprising:a socket portion defining a chamber and comprising two second engaging portions in the chamber that are engaged with the first engaging portions after the plug portion is inserted into the chamber, wherein the socket portion further comprises a block in the chamber, the block is configured to be received in a hollow space of the male building block after the plug portion is inserted into the chamber, so as to guide the male building block during insertion of the plug portion.2. (canceled)3. The female building block according to claim 1 , wherein the socket portion comprises two outer plates that are spaced apart from each other and corporately define the chamber claim 1 , the two second engaging portions are respectively arranged on the two outer plates.4. The ...

Non-fouling conducting polymers

Zwitterionic and mixed charge conducting polymers, surfaces modified to include the polymers, bulk constructs that include the polymers, and methods for using the polymers.

METHOD, APPARATUS AND SYSTEM FOR CONTROLLING ROUTING INFORMATION ADVERTISING

The application disclose a method, an apparatus and a system for controlling routing information advertising, which relates to the field of communications and is used for reducing the configuration complexity and reinforcing the operability. The method includes: receiving, by a control device, first routing information sent by a first forwarding device; wherein the first routing information includes an identifier of the first forwarding device; determining a first routing path according to the identifier of the first forwarding device, an identifier of a second forwarding device and a routing path group; and determining an advertising range of second routing information for the second forwarding device according to the first routing path; for enabling the second forwarding device to advertise the second routing information according to the advertising range of the second routing information. 1. An apparatus , comprising:a non-transitory memory storing instructions; anda processor communicably coupled to the non-transitory memory; wherein the instructions, when executed by the processor, cause the apparatus to perform:receiving first routing information, wherein the first routing information comprises an identifier of a first forwarding device;determining a first routing path according to the identifier of the first forwarding device and an identifier of a second forwarding device; anddetermining an advertising range of second routing information for the second forwarding device according to the first routing path;sending the second routing information to the second forwarding device;wherein the second routing information is generated according to the first routing information, the second forwarding device is a receiver of the second routing information, and the advertising range of the second routing information comprises an identifier of an external neighbor of the second forwarding device and the advertising range of the second routing information is used to ...

SYSTEM FOR RAPID ELECTROPHORESIS BINDING METHOD AND RELATED KITS AND COMPOSITIONS

An improved staining method and system is described for staining a biopolymer such as a peptide, a protein, an RNA, a DNA, an oligosaccharide or a complex containing a peptide, a protein, an RNA, a DNA, or an oligosaccharide in a matrix. The method includes the step of moving a staining reagent into the matrix using an electric force. The staining time can be dramatically reduced relative to conventional technologies. The improved staining method can particularly be used, for example, to stain proteins after gel separation. Other related methods, related kits and related systems for carrying out the staining method are also described. 1. A system for staining a target molecule with a staining reagent , the system comprising:(i) a matrix to be embedded with a target molecule to form a matrix embedded with the target molecule;(ii) a first solid porous material for forming a first solid porous material absorbed with a staining solution comprising the staining reagent, wherein the first solid porous material absorbed with a staining solution is to be placed in contact with the matrix embedded with the target molecule;(iii) a pair of electrodes for applying an electric force sufficient to move the staining reagent from the first solid porous material into the matrix embedded with the target molecule, thereby staining the target molecule, and to move excess free staining reagent out of the matrix, thereby destaining the matrix; and(iv) optionally, one or more additional solid porous materials absorbed with one or more buffer solutions.2. The system according to claim 1 , wherein the matrix embedded with the target molecule and the first solid porous material are interposed between the pair of electrodes claim 1 , such that the electric force moves the staining reagent in a direction such that the excess free staining reagent enters the matrix and travels the least distance in the matrix before leaving the matrix as compared to the other directions.3. The system according ...

Certificate Status Determining Method

In a method for enabling a message receive end to quickly confirm a certificate status, a defined field of a certificate includes classification information of the certificate, and a defined field of a certificate revocation list includes classification information of a revoked certificate, so that the receive end can quickly narrow a searching or matching range in massive records of the certificate revocation list based on the classification information carried in the certificate of a transmit end.

METHOD AND APPARATUS FOR ESTABLISHING TRAIL NETWORK

A method and an apparatus establish a trail in a network, where the method includes: in a process of establishing a first trail, adding, by a first ingress node to a first trail establishment response message, an IP address of the first ingress node and an optical domain; and in a process of establishing a second trail, receiving, by the first ingress node, a first trail request message sent by a second ingress node; and sending, by the first ingress node to the second ingress node in the optical domain, information about a trail that is of the first trail and is in the optical domain to which the first ingress node belongs. According to the embodiments, that a trail that is of a working trail and a protection trail are in each optical domain do not overlap can be met. 1. A method for establishing a trail in a network , wherein the network comprises: a head-end router , a tail-end router , and at least one optical domain; each optical domain comprises a first ingress node and a second ingress node; a first trail between the head-end router and the tail-end router passes through the first ingress node of each optical domain; and a second trail between the head-end router and the tail-end router passes through the second ingress node of each optical domain , wherein the method comprises:in a process of establishing the first trail, receiving, by the first ingress node, a first trail establishment request message sent by a previous-hop node, determining a trail that is of the first trail and is in an optical domain to which the first ingress node belongs, and sending, according to the determined trail, the first trail establishment request message to a next-hop node of the optical domain to which the first ingress node belongs; and receiving, by the first ingress node, a first trail establishment response message that is sent by the next-hop node according to the trail that is of the first trail and is in the optical domain to which the first ingress node belongs, ...

DATA REPORT PROCESS METHOD AND DEVICE THEREOF

A data report process method and a device thereof are disclosed. The computer technology field is related for resolving issues that the data report of prior art which can hardly satisfy self demands of various application programs with few demonstration possibilities. The method comprises: receiving and demonstrating preset element configuration information; receiving a configuration content selected in the element configuration information by an user; establishing the data report, and configuring respective elements in the data report according to the configuration content, and the data report comprises index information; obtaining data content corresponding to respective indexes in the index information according to the index information; writing the data content in the data report. The present invention is applied to data analysis service. 1. A data report process method , comprising:receiving and demonstrating preset element configuration information;receiving a configuration content selected in the element configuration information by an user;establishing the data report, and configuring respective elements in the data report according to the configuration content, and the data report comprises index information;obtaining data content corresponding to respective indexes in the index information according to the index information;writing the data content in the data report.2. The method according to claim 1 , comprising:saving the data report to a local terminal device, after establishing the data report and configuring the respective elements in the data report according to the configuration content.3. The method according to claim 1 , comprising:sending the data report to a social network server, after establishing the data report and configuring the respective elements in the data report according to the configuration content.4. The method according to claim 3 , wherein the method further comprises:downloading the data report from the social network server ...

SYSTEM FOR RAPID ELECTROPHORESIS BINDING METHOD AND RELATED KITS AND COMPOSITIONS

An improved staining method and system is described for staining a biopolymer such as a peptide, a protein, an RNA, a DNA, an oligosaccharide or a complex containing a peptide, a protein, an RNA, a DNA, or an oligosaccharide in a matrix. The method includes the step of moving a staining reagent into the matrix using an electric force. The staining time can be dramatically reduced relative to conventional technologies. The improved staining method can particularly be used, for example, to stain proteins after gel separation. Other related methods, related kits and related systems for carrying out the staining method are also described. 1. A system for staining a target molecule with a staining reagent , the system comprising:a matrix to be embedded with a target molecule to form a matrix embedded with the target molecule;(ii) a first solid porous material for forming a first solid porous material absorbed with a staining solution comprising the staining reagent, wherein the first solid porous material absorbed with a staining solution is to be placed in contact with the matrix embedded with the target molecule;(iii) a pair of electrodes for applying an electric force sufficient to move the staining reagent from the first solid porous material into the matrix embedded with the target molecule, thereby staining the target molecule, and to move excess free staining reagent out of the matrix, thereby destaining the matrix; and(iv) optionally, one or more additional solid porous materials absorbed with one or more buffer solutions.2. The system according to claim 1 , wherein the matrix embedded with the target molecule and the first solid porous material are interposed between the pair of electrodes claim 1 , such that the electric force moves the staining reagent in a direction such that the excess free staining reagent enters the matrix and travels the least distance in the matrix before leaving the matrix as compared to the other directions.3. The system according to ...

MAGNETIC BEAD PURIFICATION SYSTEM

The present invention provides a magnetic bead purification system, including: a housing; a liquid path treatment system provided inside the housing, the liquid path treatment system being connectable to a reagent barrel and a waste liquid barrel; a sample addition needle group connected to the liquid path treatment system, the sample addition needle group being movable within the housing and connected to the liquid path treatment system, so as to receive a reagent from the liquid path treatment system or to discharge a waste liquid to the liquid path treatment system; a purification magnetic separation system, including a magnetic element, the purification magnetic separation system being controllable to apply a lateral magnetic force to a purification treatment position inside the housing or stop the application of the magnetic force by the magnetic element; and a purification station system movable between a purification treatment position inside the housing and a loading position outside the housing, the purification station system being adapted to load a container. The magnetic bead purification system of the present invention can easily achieve large capacity sample treatment. 1. A magnetic bead purification system , comprising:a housing;a liquid path treatment system provided inside the housing, the liquid path treatment system being connectable to a reagent barrel and a waste liquid barrel;a sample addition needle group connected to the liquid path treatment system, the sample addition needle group being movable within the housing and connected to the liquid path treatment system, so as to receive a reagent from the liquid path treatment system or to discharge a waste liquid to the liquid path treatment system;a purification magnetic separation system, comprising a magnetic element, the purification magnetic separation system being controllable to apply a lateral magnetic force to a purification treatment position inside the housing or stop the application ...

FUNCTIONALIZED ZWITTERIONIC AND MIXED CHARGE POLYMERS, RELATED HYDROGELS, AND METHODS FOR THEIR USE

Functionalized zwitterionic and mixed charge polymers and copolymers, methods for making the polymers and copolymers, hydrogels prepared from the functionalized zwitterionic and mixed charge polymers and copolymers, methods for making and using the hydrogels, and zwitterionic and mixed charge polymers and copolymers for administration for therapeutic agents.

ZWITTERIONIC MICROGELS, THEIR ASSEMBLIES AND RELATED FORMULATIONS, AND METHODS FOR THEIR USE

Zwitterionic microgels, zwitterionic microgel assemblies, their formulations and methods for their use. 116-. (canceled)17. A method for protectively storing a population of cells , a tissue , or an organ , comprising storing a population of cells , a tissue , or an organ in a matrix comprising a zwitterionic microgel to provide stored cells , a stored tissue or a stored organ , wherein the stored cells , stored tissue , or stored organ substantially retains its biological function on storage.18. The method of claim 17 , wherein the cells are selected from pluripotent and multipotent stem and progenitor cells claim 17 , hematopoietic cells claim 17 , genetically engineered cells claim 17 , immune cells and progenitors or differentiated lineages thereof claim 17 , pancreatic islet or other insulin-producing cells claim 17 , nervous system cells and progenitors claim 17 , and cardiovascular system cells and progenitors.19. The method of claim 17 , wherein the tissue is muscle claim 17 , nerve tissue claim 17 , connective tissue claim 17 , subcutaneous tissue claim 17 , or epithelial tissue.20. The method of claim 17 , wherein the organ is kidney claim 17 , heart claim 17 , brain claim 17 , esophagus claim 17 , pharynx claim 17 , salivary glands claim 17 , stomach claim 17 , small intestine claim 17 , large intestine claim 17 , liver claim 17 , gallbladder claim 17 , pancreas claim 17 , nose claim 17 , bladder claim 17 , urethra claim 17 , arteries claim 17 , veins claim 17 , capillaries claim 17 , lymphatic vessel claim 17 , lymph node claim 17 , bone marrow claim 17 , thymus claim 17 , spleen claim 17 , gut-associate lymphoid tissue claim 17 , eye claim 17 , ear claim 17 , olfactory epithelium claim 17 , tongue claim 17 , or skin.21. The method of claim 17 , wherein the cells claim 17 , tissue claim 17 , or organ are stored in the absence of a cryoprotectant claim 17 , or in the presence of an osmolyte.22. The method of claim 17 , wherein the cells are isolated for ...

Control Channel Establishing Method, Forwarding Point, and Controller

The present invention provides a control channel establishing method, a forwarding point, and a controller. The method includes: sending, by a first FP, topology information of the first FP to a second FP by using the LLDP; receiving, by the first FP, first routing information that is sent by the controller and is used by the first FP to reach the controller, where the first routing information is generated by the controller according to the topology information of the first FP; and establishing, by the first FP, a second control channel with the controller according to the first routing information. The first FP does not need to run the IGP protocol, thereby avoiding that the first FP maintains an IGP neighbor relationship; moreover, the first FP does not need to store routing information for reaching another FP, which helps save storage resources and helps improve forwarding performance. 1. A control channel establishing method , wherein the method is applicable to a virtual cluster system , wherein the virtual cluster system comprises a first forwarding point (FP) , a second FP , and a controller , and wherein a first Link Layer Discovery Protocol (LLDP) neighbor relationship is established between the first FP and the second FP , the method comprising:sending, by the first FP, topology information of the first FP to the second FP by using the first LLDP neighbor relationship so that the second FP sends, after receiving the topology information of the first FP, the topology information of the first FP to the controller through a first control channel, wherein the first control channel is pre-established between the second FP and the controller;receiving, by the first FP, first routing information that is sent by the controller and is used by the first FP to reach the controller, wherein the first routing information is generated by the controller according to the topology information of the first FP; andestablishing, by the first FP, a second control channel with ...

METHOD, APPARATUS AND SYSTEM FOR CONTROLLING ROUTING INFORMATION ADVERTISING

A method, an apparatus and a system for controlling routing information advertising are provided, which relate to the field of communications and are used for reducing the configuration complexity and reinforcing the operability. The method includes: receiving, by a control device, first routing information sent by a first forwarding device; wherein the first routing information includes an identifier of the first forwarding device; determining a first routing path according to the identifier of the first forwarding device, an identifier of a second forwarding device and a routing path group; and determining an advertising range of second routing information for the second forwarding device according to the first routing path; for enabling the second forwarding device to advertise the second routing information according to the advertising range of the second routing information. 1. An apparatus comprising:a non-transitory memory storing instructions; anda processor communicably coupled to the non-transitory memory;wherein the instructions, when executed by the processor, cause the apparatus to perform:receiving first routing information sent by a first forwarding device;determining an advertising range of second routing information for a second forwarding device according to (a) the first routing information, (b) an identifier of the second forwarding device and (c) a routing path group,wherein the advertising range comprises an identifier of an external neighbor of the second forwarding device, and is used to enable the second forwarding device to advertise the second routing information according to the advertising range; andsending the second routing information comprising the advertising rage to the second forwarding devices.2. The apparatus according to claim 1 , wherein the instructions claim 1 , when executed by the processor claim 1 , further cause the apparatus to perform:determining a first routing path according to an identifier of the first forwarding ...

Functionalized zwitterionic and mixed charge polymers, related hydrogels, and methods for their use

Functionalized zwitterionic and mixed charge polymers and copolymers, methods for making the polymers and copolymers, hydrogels prepared from the functionalized zwitterionic and mixed charge polymers and copolymers, methods for making and using the hydrogels, and zwitterionic and mixed charge polymers and copolymers for administration for therapeutic agents.

HIGH-LOADING AND ALKALI-RESISTANT PROTEIN A MAGNETIC BEAD AND METHOD OF USE THEREOF

Provided is a high-loading and alkali-resistant protein A magnetic bead. The magnetic bead can maintain chemical stability under pH 2-14 and has an immunoglobulin G (IgG) binding capacity greater than 50 mg/mL. Further provided is a method for purifying and/or detecting an immunoglobulin, comprising a step of contacting a sample containing the immunoglobulin with the high-loading and alkali-resistant protein A magnetic bead. The alkali-resistant protein A magnetic bead can realize rapid purification of immunoglobulin, saving about 80% of treatment time and reducing total purification costs by 50%. In addition, the alkali-resistant protein A magnetic bead has high alkali resistance. An alkaline method for in situ cleaning can be performed to regenerate the magnetic bead after use. The magnetic bead has rapid magnetic response and good dispersiveness, realizing rapid magnetic bead enrichment, cleaning, and elution. The magnetic bead facilitates automated, high-throughput, and large volume purification of a sample.

METHOD AND APPARATUS FOR DETERMINING NEXT HOP AND ADVERTISING ROUTING INFORMATION

Embodiments of the present application disclose a method and an apparatus for determining a next hop and advertising routing information, which relate to the field of communications and are used for reducing operation complexity of a forwarding device and reducing network maintenance workload. The method for determining the next hop provided by the embodiments of the present application includes: receiving, by a control device, first routing information sent by a first forwarding device, where the first routing information includes an identifier of the first forwarding device; determining a first routing path according to the identifier of the first forwarding device, an identifier of a second forwarding device and a routing path group; and determining a next hop in second routing information for the second forwarding device according to the first routing path, where the second forwarding device is a receiver of the second routing information advertised by the control device. 1receiving, by a control device, first routing information sent by a first forwarding device, wherein the first routing information comprises an identifier of the first forwarding device;determining a first routing path according to the identifier of the first forwarding device, an identifier of a second forwarding device and a routing path group; anddetermining a next hop in second routing information for the second forwarding device according to the first routing path, wherein the second forwarding device is a receiver of the second routing information advertised by the control device.. A method for determining a next hop, comprising: This application is a continuation of U.S. patent application Ser. No. 14/939,880, filed on Nov. 12, 2015, which is a continuation of International Application No. PCT/CN2014/077574, filed on May 15, 2014. The International Application claims priority to Chinese Patent Application No. 201310178948.1, filed on May 15, 2013. All of the afore-mentioned patent ...

AUTOMATIC PURIFICATION SYSTEM AND BIOLOGICAL SAMPLE PURIFICATION METHOD

An automatic purification system using magnetic particles comprises: 1) a first container module; and 2) a system controller module. The first container module comprises a first container and a first magnetic field supply device disposed outside the first container. A first container liquid inlet is formed in the upper portion of the first container, and a first container liquid outlet is formed in the bottom of the first container. The system controller module can generate a variable magnetic field in the first container by controlling the first magnetic field supply device. A method for purifying a target component from a biological sample comprises a step for allowing a biological sample containing a target component to be in contact with magnetic particles capable of specifically binding the target component, in the first container in the automatic purification system. The automatic purification system and method help to efficiently separate a target product from a biological sample, and helps to reduce process costs and time at the same time. 1. An automatic purification system utilizing magnetic particles , comprising: a first container, the first container is provided with a first container liquid inlet at an upper part thereof, and is provided with a first container liquid outlet at a bottom part thereof; and', 'a first magnetic field supply device provided outside the first container; and, '1) a first container module, the first container module comprising2) a system controller module, the system controller module is capable of generating a variable magnetic field inside the first container by controlling the first magnetic field supply device.2. The automatic purification system of claim 1 , wherein the first magnetic field supply device comprises a first rotating device claim 1 , a first cantilever and a first permanent magnet claim 1 , and the first rotating device is capable of controlling the rotation of the first permanent magnet around the first ...

POSTURE DETECTION DEVICE AND NECKLACE

The present disclosure provides a posture detection device and a necklace, which relates to a field of automatic control, the posture detection device is worn on a body of a user, and the posture detection device includes a sensing device, a processor, and a reminding device. The sensing device is connected to the processor, and the sensing device obtains information of back posture changes of the body of the user and sends the information to the processor. The processor is connected to the reminding device, the processor processes the information, generates a reminding signal, and sends the reminding signal to the reminding device, the reminding device starts a vibration mode according to the reminding signal. 1. A posture detection device , worn on a body of a user , comprising:a sensing device;a processor;a switching device; anda reminding device;wherein the sensing device is connected to the processor, and the sensing device obtains information of back posture changes of the body of the user and sends the information to the processor;the processor is connected to the reminding device, and the processor processes the information of the back posture changes of the body of the user, generates a reminding signal, and sends the reminding signal to the reminding device; the reminding device starts one of reminding modes according to the reminding signal;the switching device is connected to the processor; after the reminding device starts the one of the reminding modes according to the reminding signal, the switching device obtains a switching signal and sends the switching signal to the processor; a circuit suspends detecting relevant angle signals by pulling a body of a posture detection device, so that the posture detection device temporarily turns off alerts to stay in a silent state; or the circuit is turned off or a delay time from detection to alert triggering is switched into, so that a switch of the reminding modes is realized; andthe processor instructs the ...

Inclined magnetic holder

An inclined magnetic holder is disclosed, comprising a magnetic base and a centrifuge tube support plate. The centrifuge tube support plate is provided with centrifuge tube support holes. The magnetic base comprises a first bottom plate, a fixing plate provided on the first bottom plate, and two first-side support plates respectively provided at two sides of the fixing plate. Respective top portions of the two first-side support plates are provided with a position-locating slot. A bottom surface of the position-locating slot is configured with an inclined angle. Two ends of the centrifuge tube support plate are respectively provided with a position-locating protruding block for fitting and assembling into the position-locating slot. The centrifuge tube support holes are evenly and linearly distributed on the centrifuge tube support plate. An elastic circular engagement component for holding a centrifuge tube is provided inside the centrifuge tube support holes. A block magnet is fixed to the fixing plate at a location below and corresponding to each of the centrifuge tube support holes. The block magnets below each of the centrifuge tube support holes correspond to each of the centrifuge tubes. A north pole or south pole surface of the block magnet faces the centrifuge tube and is parallel to an axis of the centrifuge tube.

Method for detecting tunnel faults and traffic engineering node

The embodiments of the present invention provide a tunnel fault detection method and a traffic engineering (TE) node. The method includes: receiving, by a traffic engineering TE node, a link fault report packet; determining, by the node, a faulty link in which a fault occurs according to the link fault report packet; detecting, by the node, whether one or more tunnels which use the node as an ingress node and pass through the faulty link exist; and identifying the tunnels as faulty tunnels if the one or more tunnels which use the node as the ingress node and pass through the faulty link exist. In the embodiments of the present invention, the TE node can detect all faulty tunnels at a time, and it is not necessary to wait until the fault notification based on each tunnel is received and then the faulty tunnel is identified one by one. Therefore, compared with the prior art, the present invention can detect the faulty tunnels in time in a network in which numerous tunnels are configured on a TE node.

Method, apparatus, and system for controlling release of route information

Microplate reader

A microreader includes: a sample holder (140) configured to support one or more samples to be analyzed; at least two xenon lamps (212) as light sources; at least two optical assemblies (132), each having one or more optical elements (720), the sample holder and each of the optical assemblies (132) being configured to direct the light from the respective xenon lamp (212) to the one or more samples to be analyzed; and detectors, each configured to receive light from the one or more samples supported by the sample holder and generate a signal responsive to the received light from the one or more samples. At least one optical element (720) of at least one of two optical assemblies (132) is configured to be removably retained in the respective optical assembly (132). The removably retained optical element (720) is retained by an assembly of magnets arranged to bias the retained optical element (720) in three-dimensions toward a reference point.

Free-standing non-fouling polymers, their compositions, and related monomers

Free-standing non-fouling polymers, their compositions, and related monomers

Free-standing non-fouling polymers and polymeric compositions, monomers and macromonomers for making the polymers and polymeric compositions, objects made from the polymers and polymeric compositions, and methods for making and using the polymers and polymeric compositions.

Automatic purification system and biological sample purification method

An automatic purification system using magnetic particles comprises: 1) a first container module; and 2) a system controller module. The first container module comprises a first container and a first magnetic field supply device disposed outside the first container. A first container liquid inlet is formed in the upper portion of the first container, and a first container liquid outlet is formed in the bottom of the first container. The system controller module can generate a variable magnetic field in the first container by controlling the first magnetic field supply device. A method for purifying a target component from a biological sample comprises a step for allowing a biological sample containing a target component to be in contact with magnetic particles capable of specifically binding the target component, in the first container in the automatic purification system. The automatic purification system and method help to efficiently separate a target product from a biological sample, and helps to reduce process costs and time.

Packet forwarding method, route sending and receiving method, and apparatus

Embodiments of this application provide a packet forwarding method, a route sending and receiving method, and related apparatus. The method is applied to a data center in a non-uniform memory access (NUMA) architecture. The data center includes a server and a network device, and the server includes a first NUMA node and a second NUMA node. The method includes: The first interface processing unit (IPU) receives a first packet from the network device, where the first packet is a packet to be sent to the first virtual machine in the first NUMA node. The first IPU sends the first packet to the first virtual machine. The second IPU receives a second packet from the network device, where the second packet is a packet to be sent to the second virtual machine in the second NUMA node. The second IPU sends the second packet to the second virtual machine.

Image sensor having an improved structure for small pixel designs

Various embodiments of the present disclosure are directed towards an image sensor. The image sensor includes a substrate having a first side and a second side. The substrate includes a pixel region. A photodetector is in the pixel region. A first doped region is in the pixel region. A second doped region is in the pixel region. The second doped region is vertically between the first doped region and the first side of the substrate. A doped well is in the substrate and laterally surrounds the pixel region. The doped well is partially in the second doped region. A portion of the second doped region is vertically between the doped well and the second side of the substrate. A trench isolation structure is in the semiconductor substrate and laterally surrounds the pixel region. A footprint of the trench isolation structure is within a footprint of the doped well.

Automatic extraction device and use method therefor

An automatic extraction device and a use method therefor. The automatic extraction device comprises a sample processing apparatus (100), a portal frame (200), a consumable assembly (300), and a pipetting apparatus (400). The sample processing apparatus (100) comprises a uniform mixing mechanism (130) and a magnetic attraction mechanism (140), which are respectively used for performing uniform mixing processing and magnetic attraction processing on a biological sample. The portal frame (200) comprises a base (201) and a moving beam (202) capable of moving relative to the base (201). The sample processing apparatus (100) and the consumable assembly (300) are provided on the base (201). The pipetting apparatus (400) is provided on the moving beam (202) for implementing a pipetting operation between the consumable assembly (300) and the sample processing apparatus (100). The automatic extraction device can be used for extracting and purifying a target biological substance, such that mechanical automation of an extraction or purification process can be achieved, thereby reducing the time and energy of operators while improving the extraction and purification efficiency.

Compositions and methods for culturing hematopoietic stem and progenitor cells

Zwitterionic microgels, their assemblies and related formulations, and methods for their use

Bildsensor mit verbesserter struktur für kleine pixeldesigns

Verschiedene Ausführungsformen der vorliegenden Offenbarung betreffen einen Bildsensor. Der Bildsensor weist ein Substrat mit einer ersten Seite und einer zweiten Seite auf. Das Substrat weist einen Pixelbereich auf. Ein Photodetektor liegt in dem Pixelbereich. Ein erster dotierter Bereich liegt in dem Pixelbereich. Ein zweiter dotierter Bereich liegt in dem Pixelbereich. Der zweite dotierte Bereich liegt vertikal zwischen dem ersten dotierten Bereich und der ersten Seite des Substrats. In dem Substrat liegt eine dotierte Wanne, die den Pixelbereich seitlich umgibt. Die dotierte Wanne liegt teilweise in dem zweiten dotierten Bereich. Ein Abschnitt des zweiten dotierten Bereichs liegt vertikal zwischen der dotierten Wanne und der zweiten Seite des Substrats. In dem Halbleitersubstrat liegt eine Grabenisolationsstruktur, die den Pixelbereich seitlich umgibt. Eine Grundfläche der Grabenisolationsstruktur liegt innerhalb einer Grundfläche der dotierten Wanne.

Method for determining certificate state

To meet real-time and security requirements in vehicle-to-everything communication, this application provides a method for enabling a message receive end to quickly confirm a certificate status. A defined field of a certificate includes classification information of the certificate, and a defined field of a certificate revocation list includes classification information of a revoked certificate, so that the receive end can quickly narrow a searching or matching range in massive records of the certificate revocation list based on the classification information carried in the certificate of a transmit end, to improve a speed and efficiency of determining the certificate status.

Sample processing apparatus and use method therefor

A sample processing apparatus (100) and a use method therefor. The sample processing apparatus (100) comprises a uniform mixing mechanism (130) and a magnetic attraction mechanism (140). The sample processing apparatus (100) further comprises at least one of a sample tube (110) and a reaction tube (120). The uniform mixing mechanism (130) is configured to perform uniform mixing processing on a biological sample in the sample tube (110) and/or a biological sample in the reaction tube (120). The magnetic attraction mechanism (140) comprises a magnet (141). The magnet (141) can move relative to the sample tube (110) and/or the reaction tube (120), so as to perform magnetic attraction processing on the biological sample in the sample tube (110) and/or the biological sample in the reaction tube (120).

Zwitterionic microgels, their assemblies and related formulations, and methods for their use

Zwitterionic microgels, zwitterionic microgel assemblies, their formulations and methods for their use.

一种茶叶分层堆放装置

本实用新型提出了一种茶叶分层堆放装置，其特征在于：包括堆放盘，连接杆、底座，温度控制层和搅拌装置，所述底座上设有若干根连接杆，所述连接杆之间设有若干个堆放盘，所述堆放盘下部设有温度控制层，所述堆放盘内部底面中央设有搅拌装置，所述搅拌装置表面设有搅拌叶片。与现有技术相比，本实用新型提供的一种茶叶分层堆放装置，结构合理，通过将茶叶依次放入堆放盘中，开启堆放盘表面的开关按钮，调节温度控制层的温度和搅拌装置的转速，使得茶叶可以在堆放盘中良好的保存堆放，多个堆放盘可以使茶叶分层放置，从而解决现有技术中茶叶堆放方式不合理，温度控制不佳等技术问题。

Certificate status determining method

In a method for enabling a message receive end to quickly confirm a certificate status, a defined field of a certificate includes classification information of the certificate, and a defined field of a certificate revocation list includes classification information of a revoked certificate, so that the receive end can quickly narrow a searching or matching range in massive records of the certificate revocation list based on the classification information carried in the certificate of a transmit end.

Rapid elisa processes and related compositions

The present invention provides improved and rapid detection methods for an antigen such as a chemical compound, a peptide, a nucleic acid, or a protein released from cells or virus particles in situ. The detection time for an antigen can be dramatically reduced relative to conventional technologies. The technology can particularly be used, for example, to modify and reduce the detection time significantly in traditional ELISA, and also Western blot or Dot blot assays. The improved ELISA method is rapid, economical, reproducible, simple and automatable. Also provided are compositions and kits for using the improved ELISA methods for the rapid detection of antigens.

Sample processing apparatus and use method therefor

A sample processing apparatus (100) and a use method therefor. The sample processing apparatus (100) comprises a uniform mixing mechanism (130) and a magnetic attraction mechanism (140). The sample processing apparatus (100) further comprises at least one of a sample tube (110) and a reaction tube (120). The uniform mixing mechanism (130) is configured to perform uniform mixing processing on a biological sample in the sample tube (110) and/or a biological sample in the reaction tube (120). The magnetic attraction mechanism (140) comprises a magnet (141). The magnet (141) can move relative to the sample tube (110) and/or the reaction tube (120), so as to perform magnetic attraction processing on the biological sample in the sample tube (110) and/or the biological sample in the reaction tube (120).

Composition Related to Rapid ELISA Process

The present invention provides improved and rapid detection methods for an antigen such as a chemical compound, a peptide, a nucleic acid, or a protein released from cells or virus particles in situ. The detection time for an antigen can be dramatically reduced relative to conventional technologies. The technology can particularly be used, for example, to modify and reduce the detection time significantly in traditional ELISA, and also Western blot or Dot blot assays. The improved ELISA method is rapid, economical, reproducible, simple and automatable. Also provided are compositions and kits for using the improved ELISA methods for the rapid detection of antigens.

Rapid elisa processes and related compositions

The present invention provides improved and rapid detection methods for an antigen such as a chemical compound, a peptide, a nucleic acid, or a protein released from cells or virus particles in situ. The detection time for an antigen can be dramatically reduced relative to conventional technologies. The technology can particularly be used, for example, to modify and reduce the detection time significantly in traditional ELISA, and also Western blot or Dot blot assays. The improved ELISA method is rapid, economical, reproducible, simple and automatable. Also provided are compositions and kits for using the improved ELISA methods for the rapid detection of antigens.

Rapid C-ELISA process and related compositions

The present invention provides improved and rapid detection methods for an antigen such as a chemical compound, a peptide, a nucleic acid, or a protein released from cells or virus particles in situ. The detection time for an antigen can be dramatically reduced relative to conventional technologies. The technology can particularly be used, for example, to modify and reduce the detection time significantly in traditional ELISA, and also Western blot or Dot blot assays. The improved ELISA method is rapid, economical, reproducible, simple and automatable. Also provided are compositions and kits for using the improved ELISA methods for the rapid detection of antigens.

Anisomycin derivative and use of anisomycin and derivative thereof as glp-1r agonist

Service packet forwarding method, device, and computer storage medium

Zwitterionic microgels, their assemblies and related formulations, and methods for their use

Method and apparatus for establishing trail network

A method and an apparatus establish a trail in a network, where the method includes: in a process of establishing a first trail, adding, by a first ingress node to a first trail establishment response message, an IP address of the first ingress node and an optical domain; and in a process of establishing a second trail, receiving, by the first ingress node, a first trail request message sent by a second ingress node; and sending, by the first ingress node to the second ingress node in the optical domain, information about a trail that is of the first trail and is in the optical domain to which the first ingress node belongs. According to the embodiments, that a trail that is of a working trail and a protection trail are in each optical domain do not overlap can be met.

Multipath transmission based packet traffic control method and apparatus

Embodiments of the present application disclose a multipath transmission based packet traffic control method, including: monitoring egress traffic of a data packet set sent by a source node; determining, according to a preconfigured mapping relationship between traffic ranges and forwarding paths, a target forwarding path set corresponding to the egress traffic of the data packet set, and a weight parameter of the egress traffic borne on each target forwarding path in the target forwarding path set; and distributing the data packet set according to the weight parameter, and transferring the data packet set to a destination node by the target forwarding path set. The embodiments of the present application further disclose a traffic control apparatus. By using the present application, centralized policing and classification can be performed on user traffic in a multipath transmission scenario.

Control channel establishing method, forwarding point, and controller

A control channel establishing method, a forwarding point, and controller. The method includes sending, by a first Forwarding Point (FP), topology information of the first FP to a second FP by using a Link Layer Discovery Protocol (LLDP), receiving, by the first FP, first routing information that is sent by the controller and is used by the first FP to reach the controller, where the first routing information is generated by the controller according to the topology information of the first FP, and establishing, by the first FP, a second control channel with the controller according to the first routing information.