23-01-2020 дата публикации
Номер: US20200024552A1
Принадлежит:
An automatic dishwashing cleaning composition having a new protease. 1. An automatic dishwashing cleaning composition comprising a protease wherein the protease is a variant having at least about 90% identity with the amino acid sequence of SEQ ID NO:1 and the variant has a glutamate (E) residue at position 39 and further comprises one or more amino acid substitutions at one or more positions selected from:(i) 3V, 4T, 8V, 9A/C/E/G/H/K/M/N/Q/W/Y, 10A/K/M/N/Q/W, 11A/I/S/T, 12A/C/D/G/M/N/R/S/T/V/W, 14D, 15D/E/F/H/I/K/M/P/Q/V/W/Y, 16L/M/S, 17C/E/F/G/I/L/N/V/W/Y, 18A/C/D/E/F/G/L/M/Q/T, 19A/C/D/E/F/H/I/K/L/N/Q/S/T/W/Y, 20A/C/D/M/N/T, 24A/E/, 25A/C/D/E/M/N, 26A/I, 33T, 36C/E/I/L/M/Q/T/V, 42C/D/E/M/Q, 43L, 44C/E/F/G/H/I/K/L/N/Q/T/V/W/Y, 47I/Y, 50I, 52A/C/D/H/L/M/N/S/T/Y, 54A/C/G/L/M/N/T/V, 55A/C/D/E/H/N/S/Y, 57D/E/H/M/N/Q/T, 59A/C/D/E/M/N/Q/T, 60S, 69S, 76A/D/E/F/H/K/L/M/N/R/T/Y, 82A, 84D/F/H/Y, 95A/N, 96M/Q, 97E/H/K, 101T, 102L/M, 104A/D/H/M/N/T/V/W/Y, 105V, 107K/M, 110L, 113T/V, 114V, 115E/H/Q, 116E/H, 118D/E/N, 120V, 128G, 129A/H/N/Y, 131A/D/E/I/M/N/P/Q/V, 133M, 135A/E/F/H/I/K/L/M/S/T/V/W/Y, 136M, 137L, 139E/S, 141E/H/N, 142A/D/E/H/M/N/Q, 143E/H/M/N/V, 144E/N, 145C, 147C, 148L/V, 150M, 156C/D/N/T, 157A/C/D/E/N/Q, 158A/C/F/L/M/N/Q/V/W/Y, 159L, 160A/C/D/M/T, 161W, 164A/K/M/Q/Y, 166D/E/I/P/Q/V, 167E, 170G, 174V, 176A/C/D/L/M/N/S, 177A/C/D/E/G/H/K/L/M/Q/S/W/Y, 178D, 179A/C/E/F/G/H/I/K/M/Q/S/V/W/Y, 180K, 182A/C/D/E/G/H/I/K/L/P/Q/T/V/W/Y, 186F, 188C/D/E/I/L/M/N/Q/S/V/W/Y, 189C/D/E, 190M, 191E, 192C/M, 193A/M, 198D/E, 200H/I/K/M/V/Y, 207K/L/N/Q/T, 209P, 210C/D/E/F/G/L/N/P/Q/Y, 211E/L/Q/R, 212A/C/Q, 218C/S, 227M/Q, 228L, 230A/D/L/M/N, 231C/E/H/I/L/N/Q/S/T, 232F/H/Q/R/W, 234A/D/E/M/T/W/Y, 236G/S/T, 238A/D/E/M/V, 239D/E/L/M/N/T, 242A, 245E, 246A/L, 247E/Q, 249C/D/E/F/I/L/S/Y, 250S/T, 253E, 254P/Y, 255A/C/D/E/F/I/M/V/W, 256C/F/H/M/W/Y, 257C/M, 259D/E/M/N, 262L, 263D/Q, 264T, 265A/M/N/Q, 266L/M/N/Q/R, 268A/C/D/E, and 269H/P/W;wherein the amino acid positions of the variant are ...
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