РЕАГЕНТ ДЛЯ ОПРЕДЕЛЕНИЯ АДЕНОЗИН-5'-ТРИФОСФАТА

Изобретение относится к области биохимии. Реагент для определения аденозин-5'-трифосфата включает люциферазу, выделенную из рекомбинантных клеток E. coli, содержащих плазмиду с геном люциферазы светляков, очищенную осаждением 35 - 75%-ным сульфатом аммония, иммобилизованную на полисахаридном носителе, люциферин, сульфат магния, смесь трис-(оксиметил)-аминометана и уксусной кислоты в качестве буферной смеси, смесь этилендиаминтетраацетата натрия с дитиотреитолом в качестве стабилизатора, дополнительный стабилизатор - смесь бычьего сывороточного альбумина и трегалозы и воду. При определении аденозин-5'-трифосфата реагент обеспечивает повышение чувствительности определения (минимальная определяемая концентрация АТФ 10-14 М), значительное уменьшение фонового сигнала. Реагент обладает стабильностью при хранении как в водной суспензии, так и в лиофилизованном состоянии. 1 з.п. ф-лы, 3 табл.

БИОКАТАЛИЗАТОР ДЛЯ ОКИСЛИТЕЛЬНОЙ ДЕСТРУКЦИИ ТИОДИГЛИКОЛЯ

Изобретение относится к биокатализаторам, которые могут быть использованы для окислительной деструкции вредных органических соединений, например тиодигликоля. Биокатализатор на основе клеток Gluconоbacter oxydans содержит в качестве носителя мезопористое углеродное волокно, насыщенное соединениями серебра или меди по всей глубине волокна. Содержание соединений металла 3-6 мас. %, а содержание клеток на волокне находится в зависимости от содержания металла в волокне, которые определяют по формуле Ск = -0,025 См2+0,235 См -0,38, где Ск - содержание клеток на волокне, г АСБ/1 г волокна; См - содержание металла в слое волокна, %. Преимуществом изобретения является повышение каталитической активности с одновременным увеличением срока службы. 2 ил., 1 табл.

СПОСОБ ПОЛУЧЕНИЯ ИММОБИЛИЗОВАННЫХ НАНОЛИПАЗ

Способ получения иммобилизованных липаз с размером частиц 250-300 нм, заключающийся в растворении носителя в растворе или в подщелоченной дистиллированной воде с добавлением фермента с липазной активностью не ниже 100 ед/г, отличающийся тем, что в качестве носителя используют пищевую карбоксиметилцеллюлозу, в качестве ферментов одну или смесь липаз, продуцируемых культурами животных панкреатических клеток или Aspergillus niger в виде порошка, иммобилизацию проводят в буферных растворах или в подщелоченной дистиллированной воде с рН 9,5-10 в присутствии 1-1,5%-ного раствора поверхностно-активного вещества при помощи интенсивного перемешивания со скоростью 4500-5000 об/мин, после чего рН реакционной смеси уменьшают до значения 3,0-3,5, выпавшие частицы вместе с ферментом отделяют и высушивают.

СПОСОБ ФЕРМЕНТАТИВНОГО ГИДРОЛИЗА ФОСФОРОРГАНИЧЕСКИХ СОЕДИНЕНИЙ В ПОЧВОГРУНТЕ

Изобретение относится к области биохимии. Предложен способ ферментативного гидролиза фосфорорганических соединений в почвогрунтах. В почвогрунт вносят биокатализатор - неочищенный полигистидинсодержащий полипептид со свойствами органофосфатгидролазы, полученный из штамма бактерий Escherichia coli ЦКМИБХ 29 и иммобилизованный на целлюлозосодержащем носителе. Биокатализатор вносят в концентрации 0,5-1,0 г/кг при pH 6,5-10,0 и температуре 15-40°С в почвогрунт, содержащий фосфорорганические соединения в концентрации до 850 мг/кг. Способ обеспечивает 100% разложение широкого спектра ФОС с высокой удельной скоростью гидролиза до 7,4 мгфос/гбиокат-ра/ч. 1 ил., 8 пр.

СПОСОБ ФОРМИРОВАНИЯ БЕЛКОВЫХ ПЛЕНОК НА ТВЕРДЫХ ПОДЛОЖКАХ

Способ формирования белковых пленок на твердых подложках путем нанесения монослоя иммобилизующего агента по технологии Ленгмюра-Блоджетт с последующей адсорбцией на монослое белковых молекул из водного раствора путем молекулярной самосборки и повторного нанесения монослоя иммобилизующего агента на поверхность образованного белкового слоя, отличающийся тем, что в качестве иммобилизующего агента используют ацетопивалинат целлюлозы.

СПОСОБ (ВАРИАНТЫ) И НОСИТЕЛЬ ДЛЯ ПРОИЗВОДСТВА ИЗОМАЛЬТУЛОЗЫ С ИСПОЛЬЗОВАНИЕМ ИММОБИЛИЗОВАННЫХ МИКРООРГАНИЗМОВ И СПОСОБ ПРОИЗВОДСТВА ИЗОМАЛЬТА ИЗ САХАРОЗЫ

... 1. Способ изомеризации сахарозы в изомальтулозу с использованием жизнеспособных иммобилизованных изомальтулозообразующих микроорганизмов, отличающийся тем, что раствор, содержащий сахарозу, приводят в контакт с жизнеспособными клетками изомальтулозообразующего микроорганизма, иммобилизованными на поверхности носителя, включающего слабоосновное анионообменное вещество в форме практически несжимаемого пористого гранулярного твердого материала, и удаляют продукт изомеризации из раствора. 2. Способ по п. 1, отличающийся тем, что его осуществляют, как непрерывный способ, выполняемый на одной или более колонок, упакованных указанным носителем. 3. Способ по п. 2, отличающийся тем, что указанные клетки микроорганизма иммобилизуют на указанной поверхности носителя путем подачи раствора микробов в указанную (указанные) колонку (колонки). 4. Способ по любому из пп. 1, 2 или 3, отличающийся тем, что плотность микробов на носителе увеличивают посредством питания иммобилизованных клеток микроорганизма ...

СПОСОБ ПОЛУЧЕНИЯ ИММОБИЛИЗОВАННЫХ НАНОПРОТЕАЗ

Способ получения иммобилизованных протеаз с размером частиц 150-200 нм, заключающийся в растворении носителя в буферном растворе или в подщелоченной дистиллированной воде с добавлением фермента с протеазной активностью не ниже 1000 ед/г, отличающийся тем, что в качестве носителя используют пищевую карбоксиметилцеллюлозу, в качестве ферментов нейтральную или щелочную протеазу из Вас.Subtilis в виде порошка или концентрированного раствора, а также панкреатин, иммобилизацию проводят в буферных растворах или в подщелоченной дистиллированной воде с рН 9,5-10 в присутствии 1-1,5%-ного раствора поверностно-активного вещества при помощи интенсивного перемешивания со скоростью 3000-5000 об/мин, после чего рН реакционной смеси уменьшают до значения 3,0-3,5, выпавшие частицы вместе с ферментом отделяют и высушивают.

Способ получения сорбента для аффинной хроматографии

Изобретение относится к получению производных целлюлозы, которые могут быть использованы в качестве сорбентов для аффинной хроматографии и биотехнологии. Изобретение позволяет повысить механическую прочность сорбентов за счет того, что целлюлозу осаждают из эмульсии 1%-ного медно-аммиачного раствора целлюлозы в смеси хлороформа и бензола при их объемном соотношении соответственно 1:9, содержащей 0,13 - 0,26 мас.% полиоксиэтиленсорбитанмоноолеата, раствором уксусной кислоты в ацетоне. Выделенный продукт обрабатывают 1,6%-ным раствором боргидрида натрия в пиридине, в течение 4 - 8 ч с последующей обработкой водным щелочным раствором, содержащим 0,01 - 0,05 мас.% эпихлоргидрина и 0,01 - 0,04 мас.% боргидрида натрия, при нагревании. Затем продукт активируют водным раствором перйодата натрия и конъюгируют с белком. 1 табл.

Способ получения иммобилизованной люциферазы светляков

Изобретение относится к биотехнологии , а именно к способу получения иммобилизованной люциферазы светляков. Цель изобретения - повы- шение удельной активности и стабильности препарата. Инкубирование люциферазы в смеси с носителем ведут в буферном растворе с ионной силой от 0,3 до 0,8, при этом активность люциферазы в растворе составляет от 0,2-10® до 20-10 мВ/мл. Носитель предварительно обрабатывают в течение 0,2 - 1,0 ч лецитином или сфин- гомиелином, или холестерином, или стеариновой кислотой, или смесью ли- пидов, выделенной из лампочек светляков . Используют 0,1 М трис-ацетат- . ный буферный раствор с рН 7,8, содер- жащий 0,3-0,8 И КС1, 15-25% глицерина , 0,8-1,2 мг/мл дитиотрейтола. 3 з.п. ф-лы. 1 табл. i (Л со 4 оо со ...

Иммобилизованная аденилатциклаза чумного микроба

Использование: биохимия и биотехнология относится к получению матриц с кова- лентно связанными белками. Сущность изобретения: получают иммобилизованную аденилатциклазу чумного микроба путем ковалентного связывания аденилатциклазы с окисленной с помощью периодата сефаро- зой CL 6B. Активность иммобилизованного фермента по отношению к нативному 95%. Активность сохраняется в течение 6 мес.

KONJUGATE MIT BIOLOGISCH ABBAUBAREM POLYMER UND VERWENDUNG DAFÜR

ADSORPTIONSMITTEL

Glycoprotein mit hemmender Aktivität gegen die Besiedlung von Helicobacter pylori

VERFAHREN ZUR REIFUNG VON BIER

ENZYMIC CATALYSTS THEIR PREPARATION AND THEIR USE IN THE PREPARATION OF 6-AMINO-PENICILLANIC ACID

After reaction with a cyanogen halide, polysaccharides form a water-soluble support for penicillin acylase. Due to its high stability, the polymer-bound penicillin acylase can be employed repeatedly for the enzymatic preparation of 6-aminopenicillanic acid from penicillins.

COMPOSITE MATERIAL

Water insoluble enzymes and method for preparing same

An enzymatically active, water-insoluble substance of the formula <FORM:1062596/C3/1> where "cell" designates a cellulose molecule, Z is an enzyme molecule, X is an -NH, -S-, imidazole or phenol group of the enzyme molecule and which is not essential to the enzymatic activity of the enzyme and n is an integer ar prepared by reacting the enzyme with a cellulose derivative cell-(-O-CO-CH2-Y)n where Y is a bromine or iodine atom. Specific examples relate to the formation of compounds comprising trypsin, chymotrypsin and ribonuclease.

Process for isomerizing glucose

Glucose is enzymatically isomerized to fructose at a temperature of from about 90 DEG C. to about 130 DEG C. by contact with chemically stabilized glucose isomerase. Chemical stabilization includes intramolecular crosslinking with a cross-linking agent, and copolymerization into a polymer matrix wherein the isomerase is attached to the polymer matrix by covalent bonds and/or hydrogen and electrostatic bonds.

Stable storage of proteins

A method of stably storing a protein, the method comprising applying a protein to be stored to a substrate which has been treated with a polyhydric compound and dried, wherein the amount of the polyhydric compound present in the substrate is sufficient to stabilise the protein, and wherein the substrate does not consist of glass. The substrate preferably comprises cellulose fibres and the polyhydric compound is selected from the group consisting of: a PVA, glycerol, sucrose, carrageenan, xanthum gum and pectin.

Process for producing glucose/fructose syrups from unrefined starch hydrolysates

A glucose/fructose syrup is produced by enzymatically isomerizing an unrefined starch hydrolysate. The hydrolysate is prepared under controlled liquefaction and saccharification conditions to provide an isomerization substrate wherein the concentrations of calcium ions and non-enzymatically generated ketose sugars are maintained at low levels.

PROCEDURE FOR THE COVERING OF WATER-SOLUBLE OR IN WATER DISPERSE-CASH PARTICLES WITH A COATING LIQUID WITH THE HELP OF THE FLUIDIZED BED TECHNOLOGY

IMMOBILISIERTES BETA-GALACTOSIDASE-ENZYM- PRAEPARAT UND VERFAHREN ZU SEINER HERSTELLUNG

PROCEDURE FOR THE CONNECTION OF ENZYME TO A CARRIER USING KATIONI COPOLYMERS AND THEREBY MANUFACTURED PRODUCT

PROCEDURE FOR THE ENZYMATIC ISOMERIZATION OF ALPHA GLUCOSE

IMMOBILIZED BETA GALACTOSIDASE ENZYME PREPARATION AND PROCEDURE FOR ITS PRODUCTION

PROCEDURE AND DEVICE FOR THE PRODUCTION OF CHEMICALLY DERIVATISIERTEN FILTER PAPERS

USE OF ENZYMHALTIGEN GRANULATES AND PROCEDURE FOR THE PRODUCTION OF A PELLETISIERTEN FODDER.

PROCEDURE FOR THE IMMOBILIZATION OF AN ENZYME IN A CELLULOSE FIBER BUNDLE FUER A FASERBUENDELDIALYSATOR FOR THE CLEANING OF BLOOD.

ENZYMES TO CELEBRATIONS A CARRIER ARE BOUND.

PROCEDURE FOR THE INCREASE OF THE ENZYME ADSORPTION.

ULTRALIGHT FOAM MATERIALS WITH OPEN PORES AND LARGE ONE SPECIFIC SURFACES AND WITH INTRODUCTIONS, FUNCTIONAL ONE GROUPS OF ION EXCHANGERS.

PROCEDURE FOR THE PRODUCTION OF DESOXYCELLULOSEVERBINDUNGEN.

ON PP OR CELLULOSE HYDRATE PROTEINS AND YOUR USE IMMOBILIZED FOR THE PRODUCTION OF BIOCATALYSTS, TESTSTREIFEN OR CHROMATOGRAPHY MATERIALS.

PROCEDURE FOR THE COVERING OF WATER-SOLUBLE OR IN WATER DISPERIGIERBAREN PARTICLES WITH A COATING LIQUID WITH THE HELP OF THE FLUIDIZED BED TECHNOLOGY

IMMOBILIZED YEAST USING METHOD FOR THE PRODUCTION OF ETHANOL AND ALCOHOLIC BEVERAGES.

MACROMOLECULAR MASSES WITH CHEMICALLY ACTIVE ONES FUELLSTOFFEN, PROCEDURE FOR YOUR PRODUCTION AND YOUR USE.

Procedure for the production of Deacylaseenzymzubereitungen

Procedure for the production of a Polynucleotids

PROCEDURE FOR THE ENZYMATIC ISOMERIZATION OF ALPHA GLUCOSE

VERFAHREN ZUR ENZYMATISCHEN ISOMERISIERUNG VON ALPHA-GLUKOSE

PROCEDURE AND SUBSTRATE FOR THE PRODUCTION OF ISOMALTULOSE OF MEANS OF IMMOBILIZED MICROORGANISMS.

PROCEDURE FOR MATURING OF BEER

Plant derived cell culture material

The present invention relates material that is useful in culturing and transferring cells as well as delivering cells. The material comprises plant derived cellulose nanofibers or derivatives thereof, wherein the cellulose nanofibers are in a form of a hydrogel or membrane. The invention also provides methods for producing these materials and compositions and uses thereof.

DISSOLUTION AND PROCESSING OF CELLULOSE USING IONIC LIQUIDS

THERMOSTABLE GLUCOSE ISOMERASE

PRODUCTION OF FRUCTOSE AND FRUCTOSE AND GLUCOSE CONTAINING SYRUP BY MEANS OF GLUCOSE ISOMERASES BEADED IN FILAMENTOUS STRUCTURES

HIGH TEMPERATURE AND ALKALINE STABLE CATALASE

The invention relates to thermal and pH stable catalases. One catalase of the invention was purified and characterized from Thermus brockianus. As a part of the characterization, the enzyme was compared to typical catalases from commercial sources and found to be significantly more thermal/alkaline stable than these other enzymes. The catalase purified from T. brockianus consists of four identical subunits having a molecular mass of approximately 42.5 kDa, for a total molecular mass of approximately 178 kDa.

ENZYME PAPER FOR THE INDICATION OF CHOLINESTERASE- INHIBITORS, THE PREPARATION AND USE THEREOF

The invention relates to an enzyme paper, which is suitable for use in the detection of cholinesterase-inhibitors in a gas or liquid, a method of using the enzyme paper for such detection and, a method of making such a paper,which method involves an advantageous immobilization and stabilization of the enzyme on the paper. The enzyme paper consists of an enzyme carrier being a type of chromatography paper with ion exchange capacity and, having immobilized on the carrier, a cholinesterase enzyme, preferably from plaice, at least one component stabilizing the cholinesterase enzyme, preferably a carbohydrate, e.g. sucrose and dextran, and a surface-active substance, e.g. Tween 80*. *Trade mark ...

BIOLOGICALLY ACTIVE MEMBRANE MATERIAL

The material includes a protein-coated microporous polymeric membrane and a biologically active agent complexed with the protein material. The polymeric microporous membrane is biologically inactive. A typical example of a biologically active agent is an enzyme. Columns formed by layers of the material to perform useful biochemical reactions are disclosed and specific tests for uric acid and glucose using the material are described as well as apparatus useful in performing the tests.

ENZYME TREATMENT

MICROBIOLOGICAL PURIFICATION OF WATER AND A MICROORGANISM FOR USE IN SAID PROCESS

The invention relates to a process for the microbiological purification of water which is polluted by contaminants such as chlorophenols. The purification is performed with the aid of microorganisms which degrade the contaminant in question, whereby the feed water is directed through a biofilter which captures the contaminant so that the water is essentially cleaned and the contaminants are enriched in said filter. Microorganisms which have been previously immobilized on the biofilter are made to degrade the contaminant captured in said biofilter. The purification of huge amounts of water is quicker and the size of the apparatus smaller when the relatively slow biodegradation can be performed as a separate second step of process. The process is especially suitable for the purification of cold waters, which normally are difficult to purify. In the present process only the water circulating in the biofilter during the biodegradation stage need be heated to a temperature which is suitable ...

PRESSURE DRIVEN ENZYME COUPLED MEMBRANES

POLYMER BEAD CONTAINING IMMOBILIZED METAL EXTRACTANT

Toxic metal contaminants dissolved in dilute aqueous solutions such as waste waters are removed by contact with water insoluble polymeric beads having an internal pore structure containing immobilized extractant material capable of sorbing the metal contaminants. The beads are prepared by forming a solution of a polymeric material in an organic solvent, blending the extractant into the solution and injection the solution through a nozzle into water to form beads. The polymeric material is preferably polysulfone or cellulose acetate, and the extractant may be yeast, algae, penicillium mold, xanthan gum, guar gum, alginate, common duckweed (Lemna sp.) or a chemical compound. A metal of value can be separated from the extractant by using dilute mineral acids or other dilute solutions to solubilize the metal.

Erzeugnis für die Durchführung biologischer Prozesse

Procédé pour fixer des composés organiques à des polymères par des liaisons covalentes

Polynucleotide phosphorylase prodn for polynucleotide prodn

Procédé pour l'obtention d'articles textiles porteurs d'enzymes

VERFAHREN ZUR HERSTELLUNG VON 6-AMINOPENICILLANSAEURE.

VERFAHREN ZUR HERSTELLUNG EINES KOVALENT GEBUNDENEN ENZYMATISCHEN KATALYSATORS.

Procédé de préparation d'un composé enzymatique

Procédé de préparation d'un carbonate de cellulose

Procédé de préparation d'une pullulanase, d'une carboxypepsidase, d'une dextranase ou de papaïne insoluble dans l'eau

Verfahren zur Herstellung von enzymatisch aktiven, wasserunlöslichen Verbindungen

Procédé de préparation d'un composé d'amylase insoluble dans l'eau

PROCEDURE FOR THE PRODUCTION OF CARRIER-BOUND ACYLASEN.

Use of filamentary structures containing encapsulated fumarase for the enzymatic production of l-malic acid

PROCEDURE FOR THE CARRIER CONNECTION BIOLOGICAL OF ACTIVE PROTEINS.

Process for the preparation of water-soluble penicillin acylase covalently bonded to polymeric supports

Process for the preparation of cellulosic fibres containing enzymes

PROCEDURE FOR THE IMMOBILIZATION OF GLYCOENZYMEN.

Preparation of penicillins and cephalosporins from precursors obtained by hydrolysis of natural penicillins/cephalosporins, using enzymatic complexes immobilised on solid carriers

The present patent describes the semisynthesis of penicillins (amoxicillin) and cephalosporins (cefalexin) from precursors (6-APA and 7-ADCA) obtained by enzymatic hydrolysis of natural penicillins and cephalosporins and the preparation of enzymatic complexes (penicillin-amidase and penicillin-acylase) immobilised on solid carriers. The immobilisation is effected on powder, fibres or film of regenerated cellulose, swollen by treatment with alkali and activated with CNBr. The hydrolysis of the natural penicillins and the semisynthesis are effected by percolating the precursors in defined buffers at controlled pH and temperature. The novelty of the present patent resides in the type of immobilisation of the penicillin-amidase and penicillin-acylase, which makes it possible to use purified enzymes and, owing to the immobilisation, to work with continuous flow. The specified support (cellulose) is inexpensive, easily available and reusable. It is therefore economically more advantageous than ...

ENZYME IMMOBILIZATION DIAPHRAGM, PROCEDURE FOR THE PRODUCTION THE SAME AND USE OF THE DIAPHRAGM IN AN ELECTRO-CHEMICAL MEASURING DEVICE.

PROCEDURES FOR THE PRODUCTION A TO KOVALENTEN CONNECTION WITH BIOLOGICALLY ACTIVE MATERIAL ENABLING SUBSTRATE.

Immobilized enzymes.

TREATMENT OF BIOMASS

METHOD FOR MATURATION OF BEER METHOD FOR MATURATION OF BEER

СПОСОБ ПОЛУЧЕНИЯ ИММОБИЛИЗОВАННЫХ МИКРООРГАНИЗМОВ

Изобретение относится к способу получения иммобилизованных микроорганизмов, включающему облучение волокнистого материала пучком электронов в атмосфере воздуха при полной дозе, составляющей по меньшей мере 5 Мрад, для получения функционализированного волокнистого материала, имеющего карбоксильные группы, и обеспечение контактирования указанного функционализированного волокнистого материала с микроорганизмами, например дрожжами и/или бактериями.

Woven fabric carrier for cell immobilization and preparation method thereof

The invention discloses a woven fabric carrier for cell immobilization and a preparation method thereof and belongs to the field of biological engineering. The preparation method of the woven fabric carrier comprises selecting a fiber from one of dacron, nylon, cotton and hemp; designing a fabric weave to enable the fabric weave to be a compound double-layer weave of random two of a basket weave, a honeycomb weave, a through hole weave and a crepe weave; selecting weaving parameters; and performing postprocessing, using a sewing or bundling method to process a woven fabric discharged out of a machine into a cylinder with the diameter of 2-7cm, and reserving after sterilization. The woven fabric carrier is used for immobilization of terephthalic acid (TA) degradation bacterium testosterone comamonas, and the degradation rate of the TA is greatly improved in comparison with a control sample which is not immobilized.

A method for utilizing the maturing preparation wine immobilized yeast method

Method for utilizing lactobacillus fermented agricultural and sideline product to prepare gamma-aminobutyric acid

利用乳酸菌发酵农副产品制备γ‑氨基丁酸的方法，属于农副产品生物加工技术领域。其步骤包括：S1、配制乳酸菌悬菌液；S2、乳酸菌固定；S3、将所述步骤S2得到的固定化乳酸菌接种到含农副产品的液体培养基中，充分搅拌，进行液体发酵。本发明中利用丝胶蛋白、纤维素和含多醛基的氧化葡聚糖来固定乳酸菌，具有生物相容性高、安全无毒、稳定性好、促进乳酸菌增殖等特点；利用废弃的农副产品作为发酵培养底物，可实现资源循环利用，得到的发酵液中既含有农副产品的营养物质，又含有大量γ‑氨基丁酸，具有很高的营养价值。

A biological legal detergent cotton products

PROCESS FOR OBTAINING COMPOSITIONS CONTAINING DERIVED FROM GLYCOPROTEINES, PRODUCTS RESULTING FROM THIS PROCESS AND THEIR APPLICATIONS

PROCESS OF REGENERATION OF SUPPORTS Of ENZYMES

PROCEEDED FOR the PREPARATION Of a CARRYING SUBSTANCE READY TO FORM a COVALENT BOND WITH a SUBSTANCE ACTIVITY HAS BIOLOGICAL

COMPOSITIONS ENZYMATIQUES POUR L'ISOMERISATION DU GLUCOSE EN LEVULOSE

L'invention concerne de nouvelles compositions enzymatiques contenant de la glucose-isomérase intracellulaire incluse dans des structures à base d'ester de cellulose, leur préparation et leur utilisation pour isomériser le glucose en lévulose. Ces compositions contiennent, outre les cellules de microorganismes, par exemple Streptomyces phaeochromogènes, un composé du magnésium peu soluble dans l'eau. Ces compositions permettent d'isomériser en continu du glucose en lévulose sans adjonction d'ion magnésium au sirop de glucose à isomériser.

METHOD OF PREPARATION OF COPOLYMERS OF POLYSACCHARIDES IN the CAPACITY AS SUPPORTS Of ENZYMATIC ACTIVITY AND COPOLYMERS THUS OBTAINED

ENZYMATIC COMPOSITIONS FOR the ISOMERIZATION OF LEVULOSE GLUCOSE

SUBSTRATE FOR IMMOBILIZING FUNCTIONAL SUBSTANCES AND METHOD FOR PREPARING THE SAME

A substrate having compounds disposed thereon for immobilizing a functional molecule, each compound having a chain including: a moiety R that is chemically coupled to the substrate, the moiety R being selected from the group consisting of an ether, ester, carbonyl, carbonate ester, thioether, disulfide, sulfinyl, sulfonyl, and carbonothioyl; and an epoxide-containing moiety that is coupled to the moiety R by a linker including at least one nucleophilic group. Methods of preparing the substrate and use of the substrate are also disclosed 121-. (canceled)23. The method as claimed in claim 22 , further comprising the step of applying a substantially homogenous mixture of stabilizing additives to the surface of the substrate wherein said additives are selected to stabilize said functional molecule.24. The method as claimed in claim 23 , wherein the step of applying the substantially homogenous mixture of additives comprises evaporating a solvent of a solution of said additives onto the substrate.25. The method as claimed in claim 23 , wherein the stabilizing additives are selected from the group consisting of a sugar claim 23 , an organic acid claim 23 , an amino acid claim 23 , a sugar acid and a thiol.26. A method of preparing a substrate comprising immobilized functional molecules claim 23 , the method comprising the steps of:(i) providing electrophilic compounds coupled to the surface of the substrate;(ii) allowing the electrophilic compounds to undergo a nucleophilic substitution reaction to provide a nucleophilic group thereon and thereby increase the nucleophilicity of the substrate surface;(iii) allowing the nucleophilic group to undergo a nucleophilic substitution reaction with another electrophilic compound to provide an electrophilic group on the substrate surface and thereby increase the electrophilicity of the substrate;each electrophilic compound from step (iii) being coupled to said functional molecules, wherein the functional molecules comprise enzymes ...

Immobilized organophosphate-degrading enzymes and methods of making the same

The present application discloses immobilized enzymes and immobilized enzyme materials comprising a crosslinked organophosphate-degrading enzyme having a support material which includes a biomass material and/or a polymeric material. The resulting immobilized enzyme materials may be biodegradable. The present application also discloses methods of making and using the disclosed immobilized organophosphate hydrolase enzyme and enzyme materials.

Plant derived cell culture material

The present invention relates material that is useful in culturing and transferring cells as well as delivering cells. The material comprises plant derived cellulose nanofibers or derivatives thereof, wherein the cellulose nanofibers are in a form of a hydrogel or membrane. The invention also provides methods for producing these materials and compositions and uses thereof.

SUBSTRATE FOR IMMOBILIZING FUNCTIONAL SUBSTANCES AND METHOD FOR PREPARING THE SAME

A substrate having compounds disposed thereon for immobilizing a functional molecule, each compound having a chain including: a moiety R that is chemically coupled to the substrate, the moiety R being selected from the group consisting of an ether, ester, carbonyl, carbonate ester, thioether, disulfide, sulfinyl, sulfonyl, and carbonothioyl; and an epoxide-containing moiety that is coupled to the moiety R by a linker including at least one nucleophilic group. Methods of preparing the substrate and use of the substrate are also disclosed. 1. A method of preparing a substrate comprising immobilized functional molecules , the method comprising the steps of:(i) Providing electrophilic compounds coupled to the surface of the substrate;(ii) Allowing the electrophilic compounds to undergo a nucleophilic substitution reaction to provide a nucleophilic group thereon and thereby increase the nucleophilicity of the substrate surface;(iii) Allowing the nucleophilic group to undergo a nucleophilic substitution reaction with another electrophilic compound to provide an electrophilic group on the substrate surface and thereby increase the electrophilicity of the substrate;each electrophilic compound from step (iii) being coupled to a functional molecule,wherein the functional molecule is selected from a group consisting of an affinity ligand, a chelator, a catalyst, an ion exchanger, a dye and an indicator.2. The method as claimed in claim 1 , wherein the substrate further comprising a coating disposed on said substrate claim 1 , the coating comprising a substantially homogenous mixture of stabilizing additives selected to stabilize said functional molecule claim 1 , wherein the stabilizing additives are selected from the group consisting of a sugar claim 1 , an organic acid claim 1 , an amino acid claim 1 , an a sugar acid.3. The method as claimed in claim 1 , wherein the functional molecule is chiral4. The method as claimed in claim 1 , wherein the functional molecule is a ...

SYSTEMS AND METHODS FOR GROWING A BIOFILM OF PROBIOTIC BACTERIA ON SOLID PARTICLES FOR COLONIZATION OF BACTERIA IN THE GUT

The present invention provides a method, wherein the method forms a biofilm, wherein the biofilm comprises a population of at least one bacterial strain attached to particles, wherein the biofilm is configured to colonize a gut of a subject in need thereof for at least five days, when ingested by the subject, the method comprising: a. obtaining a population comprising at least one strain of bacteria; b. inoculating a growth medium containing particles with the population comprising at least one strain of bacteria; c. incubating the particles with the population comprising at least one bacterial strain for a time sufficient for the population of at least one strain of bacteria to attach to the particles; and d. culturing the population comprising at least one strain of bacteria attached to the particles in a growth medium, for a time sufficient to form a biofilm. 2. The method of claim 1 , wherein the population of at least one strain of bacteria attached to the particles is first cultured in the growth medium under static conditions claim 1 , followed by culture in the growth medium under flow conditions.3. The method of claim 1 , wherein the particles are porous particles ranging from 30 to 500 microns in diameter.4. The method of claim 1 , wherein the particles are selected from the group consisting of: seeds claim 1 , dicalcium phosphate claim 1 , and cellulose.5. The method of claim 1 , wherein said particle is a porous particle.6. The method of claim 1 , wherein the population comprising at least one bacterial strain is selected from gut microflora.7. The method of claim 1 , wherein said biofilm is acid tolerant.8. The method of claim 1 , wherein said biofilm is configured for pH dependent targeted release of the bacterial biofilm in the gastrointestinal tract.9. The method of claim 1 , wherein the biofilm is encapsulated with a compound configured to release the at least one bacterial strain at a pH found in the intestine of an animal.10. The method of claim 9 ...

COMPOSITION FOR EMBEDDED MICROBIAL CULTURE

Disclosed is a composition for embedded three-dimensional microbial culture, the composition including nanofibrillar cellulose and at least one nutrient source. Also disclosed is a method for the manufacture of a composition for embedded three-dimensional microbial culture, the method including the steps of providing nanofibrillar cellulose, mixing the nanofibrillar cellulose with water and at least one nutrient source and optional additives to obtain a mixture, and optionally drying the mixture. 120-. (canceled)21. A composition for embedded three-dimensional microbial culture , said composition comprising nanofibrillar cellulose and at least one nutrient source.22. The composition according to claim 21 , wherein the nanofibrillar cellulose is selected from plant derived nanofibrillar celluloses and microbial nanofibrillar celluloses.23. The composition according to claim 21 , wherein the nanofibrillar cellulose is selected from native nanofibrillar celluloses and chemically modified nanofibrillar celluloses.24. The composition according to claim 21 , wherein the nanofibrillar cellulose is native ion-exchanged nanofibrillar cellulose.25. The composition according to claim 21 , wherein the composition comprises 0.05-80 wt % of nanofibrillar cellulose.26. The composition according to claim 21 , wherein the composition comprises water and optional additives.27. The composition according to claim 21 , wherein the composition is in the form of hydrogel or powder.28. A method for the manufacture of a composition for embedded three-dimensional microbial culture claim 21 , said method comprising the steps of providing nanofibrillar cellulose claim 21 , mixing said nanofibrillar cellulose with water and at least one nutrient source and optional additives to obtain a mixture claim 21 , and optionally drying said mixture.29. The method according to claim 28 , wherein the nanofibrillar cellulose is selected from plant derived nanofibrillar celluloses and microbial ...

Production method for immobilized microorganisms and production method for amino acid using the same

An object of the present invention is to provide a method for producing an immobilized microorganism having high filtration properties, and to provide a method for producing an amino acid using the immobilized microorganism. A method for producing an immobilized microorganism is characterized in that a microorganism is contacted with carboxymethyl cellulose sodium salt and then contacted with polyethylenimine and an alkane dial after the first contact with carboxymethyl cellulose sodium salt.

METHOD FOR EX VIVO TREATING BLOOD OR PLASMA

A method for ex vivo treating blood or plasma is provided. The method includes (a) ex vivo contacting a blood or plasma with an enzyme composition to react the enzyme composition with the blood or plasma, wherein the enzyme composition is capable of eliminating electronegative low-density lipoprotein from the blood or plasma by the activity of the enzyme composition, and the enzyme composition is selected from a group consisting of: a first enzyme for eliminating a glycan residue of an electronegative low-density lipoprotein (LDL); a second enzyme for eliminating ceramide carried by a electronegative low-density lipoprotein (LDL); and a combination thereof; and (b) terminating contact between the blood or plasma and the enzyme composition to terminate the reaction of the enzyme composition with the blood or plasma. 1. A method for ex vivo treating blood or plasma , comprising: a first enzyme for eliminating a glycan residue of an electronegative low-density lipoprotein (LDL);', 'a second enzyme for eliminating ceramide carried by a electronegative low-density lipoprotein (LDL); and', 'a combination thereof; and, '(a) ex vivo contacting a blood or plasma with an enzyme composition to react the enzyme composition with the blood or plasma, wherein the enzyme composition is capable of eliminating electronegative low-density lipoprotein from the blood or plasma by the activity of the enzyme composition, and the enzyme composition is selected from a group consisting of(b) terminating contact between the blood or plasma and the enzyme composition to terminate the reaction of the enzyme composition with the blood or plasma.2. The method for ex vivo treating blood or plasma as claimed in claim 1 , wherein the step (a) is performed for about 0.25-8 hours.3. The method for ex vivo treating blood or plasma as claimed in claim 1 , wherein the step (a) is performed at about 4-40° C.4. The method for ex vivo treating blood or plasma as claimed in claim 1 , wherein the step (a) is ...

CARRIER FOR ENZYME IMMOBILIZATION USE, AND IMMOBILIZED ENZYME

This invention provides a novel carrier for enzyme immobilization and an immobilized enzyme. The carrier for enzyme immobilization according to an embodiment comprises a porous material and cellulose that has an amino group-containing substituent at an anomeric position and is immobilized on the porous material. The immobilized enzyme contains the carrier for enzyme immobilization and an enzyme immobilization on the cellulose. The carrier for enzyme immobilization is obtained by adding an acid to an aqueous solution in which cellulose having an amino group-containing substituent at an anomeric position is dissolved in an aqueous alkaline solution to deposit the cellulose in the presence of a porous material. The immobilized enzyme is obtained by immobilizing an enzyme on the cellulose. 1. A carrier for enzyme immobilization comprising a porous material and cellulose , the cellulose having an amino group-containing substituent at an anomeric position and being immobilized on the porous material.3. The carrier for enzyme immobilization according to claim 1 , wherein the porous material comprises a fibrous material.4. An immobilized enzyme comprising the carrier for enzyme immobilization of claim 1 , and an enzyme that is immobilized on the cellulose.5. A method for producing a carrier for enzyme immobilization claim 1 , comprisingadding an acid to an aqueous solution in which cellulose having an amino group-containing substituent at an anomeric position is dissolved in an aqueous alkaline solution to deposit the cellulose in the presence of a porous material to thereby immobilize the cellulose on the porous material.6. A method for producing an immobilized enzyme claim 1 , comprisingadding an acid to an aqueous solution in which cellulose having an amino group-containing substituent at an anomeric position is dissolved in an aqueous alkaline solution to deposit the cellulose in the presence of a porous material to thereby immobilize the cellulose on the porous ...

BIOCHEMISTRY REACTIVE MATERIAL AND DEVICE FOR ELIMINATING ELECTRONEGATIVE LOW-DENSITY LIPOPROTEIN (LDL) AND METHOD FOR TREATING BLOOD OR PLASMA EX VIVO TO ELIMINATE ELECTRONEGATIVE LOW-DENSITY LIPOPROTEIN THEREIN

The present disclosure provides a biochemistry reactive material, including a substrate and an enzyme composition immobilized on the substrate. The enzyme composition is selected from a group consisting of a first enzyme, a second enzyme, and a combination thereof. The first enzyme is used for eliminating a glycan residue of an electronegative low-density lipoprotein (electronegative LDL). The second enzyme is used for eliminating ceramide carried by an electronegative low-density lipoprotein. The biochemistry reactive material is capable of eliminating electronegative low-density lipoprotein. 1. A biochemistry reactive material , comprising:a substrate; and a first enzyme for eliminating a glycan residue of an electronegative low-density lipoprotein (electronegative LDL);', 'a second enzyme for eliminating ceramide carried by an electronegative low-density lipoprotein; and', 'a combination thereof,, 'an enzyme composition immobilized on the substrate, wherein the enzyme composition is selected from a group consisting ofwherein the biochemistry reactive material is capable of eliminating electronegative low-density lipoprotein.2. The biochemistry reactive material as claimed in claim 1 , wherein the substrate comprises silica gel claim 1 , cellulose claim 1 , diethylaminoethyl cellulose (DEAE cellulose) claim 1 , chitosan claim 1 , polystyrene claim 1 , polysulfone claim 1 , polyethersulfone claim 1 , acrylate resin or polysaccharide.3. The biochemistry reactive material as claimed in claim 1 , wherein the substrate has a particle structure or a hollow-tube structure.4. The biochemistry reactive material as claimed in claim 1 , wherein the substrate is a cellulose bead.5. The biochemistry reactive material as claimed in claim 1 , wherein the substrate is a chitosan bead.6. The biochemistry reactive material as claimed in claim 1 , wherein the substrate is a cellulose hollow fiber claim 1 , a polysulfone hollow fiber claim 1 , epoxy acrylic resin or a ...

Blood Treatment With Inactivation Of Circulating Nucleic Acids

The present invention relates to a device for the treatment of blood comprising a solid phase on which a polypeptide is immobilized which is suitable for the inactivation of free nucleic acids. Suitable polypeptides are, for example, deoxyribonucleases, ribonucleases, DNA methyltransferases or cytosine deaminases. The invention further comprises the use of such devices for the treatment of patients suffering from chronic kidney failure, cancer or lupus erythematosus, as well as methods and systems for the treatment of blood, wherein free nucleic acids are inactivated outside the body. 1. A device for the treatment of blood , comprising(a) tubes for whole blood or blood plasma to flow through from and to the patient,(b) a solid phase on which a polypeptide is immobilized which is suitable for the inactivation of free nucleic acids.2. The device according to claim 1 , wherein the device furthermore comprises (c) a dialyzer or haemofilter.3. The device according to claim 1 , wherein the polypeptide is selected from the group consisting of deoxyribonucleases claim 1 , ribonucleases claim 1 , endonucleases claim 1 , exonucleases claim 1 , endoribonucleases claim 1 , exoribonucleases or peptides with nuclease activity.4. The device according to claim 1 , wherein the polypeptide is selected from the group consisting of DNA methyltransferases 1 (DNMT1) or peptides with methyltransferase activity.5. The device according to claim 1 , wherein the polypeptide has a cytosine deaminase activity.6. The device according to claim 2 , wherein the solid phase is upstream of the dialyzer or haemofilter.7. The device according to claim 2 , wherein the solid phase is located within the dialyzer or haemofilter.8. The device according to claim 1 , wherein the device furthermore comprises (d) a plasma filter.9. The device according to claim 1 , wherein the solid phase on which the polypeptide is immobilized comprises a hollow fibre membrane claim 1 , beads or a non-woven.10. The device ...

BIODEGRADABLE IMMOBILIZED ENZYMES AND METHODS OF MAKING THE SAME

The present application discloses immobilized enzymes and immobilized enzyme materials comprising a crosslinked enzyme having a support material which includes a biomass material different than the biomass used to initially derive the enzyme. Optionally, the immobilized enzyme further includes a polymeric material and/or the biomass which was used to initially derive the enzyme. The resulting immobilized enzyme materials may be biodegradable. The present application also discloses methods of making and using the disclosed immobilized enzyme materials. 1(a) the biomass material, different from the biomass material the enzyme was derived from, has not been gelled;(b) the crosslinked enzyme was formed by reacting the enzyme with a crosslinking material in the presence of the biomass material, and(c) the immobilized enzyme material has a particulate form and exhibits enzymatic activity.. An immobilized enzyme material comprising a crosslinked enzyme having a support matrix that includes a biomass material different from the biomass material the enzyme was derived from, wherein; This application is a continuation of application Ser. No. 13/675,659 filed on Nov. 13, 2012, entitled BIODEGRADABLE IMMOBILIZED ENZYMES AND METHODS OF MAKING THE SAME, which claims priority to U.S. Provisional Patent Application No. 61/558,758 filed on Nov. 11, 2011, and incorporates all by reference herein, in their entirety.The claimed technology relates generally to biocatalysts such as enzymes and more specifically to the immobilization of enzymes.Immobilized biocatalysts have found applications in a variety of industries where specific chemical conversions are required. Specific large scale examples in the food and pharmaceutical industries include immobilized glucose isomerase for the conversion of glucose to fructose and immobilized penicillin acylase for the preparation of derivatives of penicillin. Immobilized enzymes can be used for large scale bioremediation such as the destruction of ...

Filter

A filtration material is disclosed that may include a first layer comprising a first three-dimensional network of fibers including a first plurality of cells, including an extracellular matrix comprising α-glucan and chitin. The filtration material may include a second layer fluidly downstream of the first layer comprising a second three-dimensional network of fibers including a second plurality of cells, including an extracellular matrix including α-glucan and chitin and the second layer may be adhered to the first layer. The first layer and the second layer may be coated with a polymer mixture including a polymer configured to mitigate exposure of the first and second three-dimensional network of fibers to glucanases and chitanases and further resist thermal degradation below a predetermined temperature. The polymer mixture may further include an antioxidant at an amount sufficient to mitigate the polymer from thermally degrading below the predetermined temperature.

A medical product comprising a bioactive molecule immobilized to nanofibrillar cellulose, and a method for preparing thereof

The present application provides a method for preparing a medical product for covering tissue, the method comprising providing nanofibrillar cellulose, providing a bioactive molecule, and covalently bonding the bioactive molecule to the nanofibrillar cellulose. The present application also provides a medical product for covering tissue comprising a bioactive molecule covalently bound to nanofibrillar cellulose. 1. A method for preparing a medical product for covering tissue , such as skin , the method comprisingproviding nanofibrillar cellulose,providing a bioactive molecule, andcovalently bonding the bioactive molecule to the nanofibrillar cellulose.2. The method of claim 1 , comprising preparing at least one layer comprising the nanofibrillar cellulose.3. The method of claim 1 , comprising carrying out the covalent bonding through primary amines or through sulfhydryl groups.4. The method of claim 1 , comprising carrying out the covalent bonding in an aqueous medium.5. The method of claim 1 , wherein the bioactive molecule is selected from proteins claim 1 , such as enzymes claim 1 , peptides claim 1 , nucleic acids claim 1 , hormones claim 1 , cytokines claim 1 , photosensitizing molecules claim 1 , and anti-cancer drugs.6. The method of claim 1 , wherein the bioactive molecule is a quorum quenching protein claim 1 , such as an acylase claim 1 , an amidase claim 1 , an amylase claim 1 , Subtilisin A claim 1 , a lactonase claim 1 , or an oxidoreductase.7. The method of claim 1 , comprising providing the nanofibrillar cellulose at a moisture content of claim 1 , or adjusting the moisture content of the nanofibrillar cellulose to the range of 0-20% (w/w) claim 1 , such as to the range of 1-10% (w/w) claim 1 , for example to the range of 5-7% (w/w).8. The method of claim 1 , comprising providing a gauze claim 1 , and incorporating the nanofibrillar cellulose to the gauze.9. The method of claim 1 , wherein the nanofibrillar cellulose claim 1 , when dispersed in water ...

PROCESSING BIOMASS

Biomass (e.g., plant biomass, animal biomass, and municipal waste biomass) is processed for use in the production of useful products, such as fuels. For example, systems can use biomass materials, such as cellulosic and/or lignocellulosic materials, to enhance the production of a product, e.g., the production of ethanol and/or butanol by fermentation. 1. A method comprising:irradiating a fibrous material with a beam of electrons in air to produce a functionalized fibrous material having carboxylic acid groups extending outwardly therefrom, andcontacting the functionalized fibrous material with organisms such that the organisms become immobilized on the fibrous material.2. The method of wherein the fibrous material comprises a lignocellulosic material.3. The method of wherein the material comprises corn cobs and/or corn stover.4. The method of wherein the electrons are accelerated.5. The method of wherein the organisms comprise fermentative organisms.6. The method of wherein the organisms comprise yeast and/or bacteria.7. The method of further comprising claim 1 , after immobilization of the organisms on the fibrous material claim 1 , placing the fibrous material in an aqueous medium containing one or more low molecular weight sugars. This application is a continuation application of U.S. application Ser. No. 14/925,850, filed Oct. 28, 2015, which is a continuation application of U.S. application Ser. No. 13/964,354, filed Aug. 12, 2013, now U.S. Pat. No. 9,206,413, issued Dec. 8, 2015, which is a continuation application of U.S. application Ser. No. 13/662,763 filed Oct. 29, 2012, now U.S. Pat. No. 8,597,917, issued on Dec. 3, 2013, which is a continuation application of U.S. application Ser. No. 12/782,543 filed May 18, 2010, now U.S. Pat. No. 8,377,668, issued Feb. 19, 2013, which claimed priority to U.S. Provisional Application Ser. No. 61/180,032, filed May 20, 2009, and U.S. Provisional Application Ser. No. 61/252,293, filed Oct. 16, 2009. The complete ...

PROTEIN PRODUCTION METHOD

The present invention provides a production method of a protein including suspension culture of a cell having protein production ability in a medium composition containing nanofiber under physical disruption conditions and in a state of being attached to the nanofiber. 1. A method for producing a protein , comprising culturing a cell having protein production ability in suspension in a medium composition comprising a nanofiber under physical disruption condition and in a state of being attached to the nanofiber.2. The method according to claim 1 , wherein the nanofiber is constituted of a non-water-soluble polysaccharide selected from the group consisting of cellulose claim 1 , chitin and chitosan.3. The method according to claim 1 , wherein the nanofiber is a chitin nanofiber.4. The method according to claim 3 , wherein the content of the chitin nanofiber in the medium composition is 0.003-0.1% (weight/volume).5. The method according to claim 1 , wherein the cell is an adherent cell.6. A method for proliferating a cell claim 1 , comprising culturing a cell in suspension in a medium composition comprising a nanofiber under physical disruption condition and in a state of being attached to the nanofiber.7. The method according to claim 6 , wherein the nanofiber is constituted of a non-water-soluble polysaccharide selected from the group consisting of cellulose claim 6 , chitin and chitosan.8. The method according to claim 6 , wherein the nanofiber is a chitin nanofiber.9. The method according to claim 8 , wherein the content of the chitin nanofiber in the medium composition is 0.003-0.1% (weight/volume).10. The method according to claim 6 , wherein the cell is an adherent cell.11. The method according to claim 2 , wherein the cell is an adherent cell.12. The method according to claim 3 , wherein the cell is an adherent cell.13. The method according to claim 4 , wherein the cell is an adherent cell.14. The method according to claim 7 , wherein the cell is an adherent ...

Method for the generation and cultivation of a plant cell pack

The present invention relates to the generation and cultivation of plant cell material in the form of a non-tissue multilayer cell pack and its use for the accumulation or harvesting of a desired product. In particular, the invention provides a method for the generation of plant cell material in the form of a medium-deprived, porous structured and non-tissue multilayer cell pack and for the subsequent maintenance of said cell pack, comprising the steps of (i) providing a cell pack by separating cells from a plant cell suspension culture, wherein the content of the liquid comprised by the cell pack is reduced and adjusted to correspond to a cell pack density between 0.1 and 0.9 g wet cell weight per cm3, thereby establishing the medium-deprived and porous structured nature of said cell pack, and (ii) incubating said medium-deprived and porous structured cell pack in a non-liquid environment under a relative humidity of 50 to 100%.

CONSORTIUM OF FUNGI IMMOBILIZED ON A LAMINAR LIGNOCELLULOSE CARRIER FOR THE TREATMENT OF WASTEWATER AND METHOD FOR PRODUCING SAME

The invention relates to a laminar biocarrier made by weaving or interlacing yarns of lignocellulosic material, which supports and immobilizes a consortium of wood-decay fungi, in particular strains of and , for the treatment of wastewater contaminated by colourants, heavy metals, chemical oxygen demand and biological oxygen demand. The invention also relates to a method for producing the inoculated laminar biocarrier and to the use thereof as a filter for reactors of different configurations for the treatment of waste effluents. 1. A laminar biocarrier for the treatment of wastewaters with an elastic , flexible and resistant mesh shape , characterized in that it is prepared by weaving or interlacing lignocellulosic yarns , and holds and immobilizes a wood-decay fungi biomass layer.2P. ostreatus, P. chrysosporium, Trametesversicolor, Ganodermalucidum, Lentulaedodes, Phlebia radiataIrpexlacteus.. The laminar biocarrier of claim 1 , characterized in that the immobilized wood-decay fungi fungal are selected from the group consisting of and3P. ostreatusP. chrysosporium.. The laminar biocarrier of claim 2 , characterized in that the immobilized wood-decay fungi strains are claim 2 , and4. The laminar biocarrier of claim 1 , characterized in that the biomass layer in areas are equivalent to a ratio of 0.01 to 0.5% (mass/volume) over the entire laminar biocarrier.5. A filter for treating wastewaters characterized in that it comprises:{'claim-ref': {'@idref': 'CLM-00001', 'claim 1'}, '(a) a laminar biocarrier according to ; and'}(b) a support allowing the adjustment and assembly of the laminar biocarrier to the reactor for carrying out the treatment.6. Method for preparing the laminar biocarrier of characterized in that it comprises the steps of:1. Getting the support ready, which involves taking the previously washed and dried lignocellulosic material from about 1 to 10 mm to form a mesh of any shape, preferably square, with a pore size between 0.1 mm and 10 mm;2. ...

METHOD FOR PREPARING KAOLIN IMMOBILIZED GY2B BACTERIA AND APPLICATION THEREOF

Provided are a method for preparing kaolin immobilized GY2B bacteria and use thereof. 1. A method for preparing immobilized GY2B bacteria , comprising the steps of:activating a GY2B bacteria culture: growing the activated GY2B bacterial culture in a phenol-containing enrichment culture solution, so as to obtain a concentrated bacterial solution centrifuging the concentrated bacterial culture; washing the concentrated bacterial culture with a sterile physiological saline; suspend the washed concentrated bacterial culture in a sterile physiological saline so as to obtain a bacterial suspension;adding to the bacterial suspension a sterilized kaolin suspension comprising kaolin dissolved in a sterile MSM culture solution. in a inoculation amount of 2˜8%, adsorbed and immobilized for 1˜4 hr, so as to prepare the kaolin immobilized GY2B bacteria.2. A method according to , wherein the activation and cultivation of the GY2B bacteria in is carried out as follows: 1˜2 GY2B colonies stored in a plate are picked into a sterile MSM culture solution containing 50˜200 mg/L phenol , shaken and cultured at 25˜35° C. , 100˜200 r/min , in dark , for 8˜16 hr , then 1˜2 mL of the bacterial solution is taken , sub-cultured in a fresh sterile MSM culture solution containing 50˜200mg/L phenol , for 8˜16 hr , then 1˜2 ml of the well grown bacterial solution is taken , and sub-cultured in a fresh sterile MSM culture solution containing 50˜200mg/L phenol , and repeated the sub-culturing as such 2-5 times , so as to obtain a GY2B bacteria having an ability to stably degrade phenol , which is plated and stored.3. A method according to claim 1 , wherein the bacteria are grown in an enrichment culture solution containing 50˜200 mg/L phenol for 8˜16 h so as to obtain the concentrated bacterial solution.4. A method according to claim 1 , wherein the concentration of the bacterial suspension obtained is adjusted in an ultraviolet spectrophotometer at 600 nm to make the absorbance of A=0.8˜1.5.5. A ...

COMPOSITIONS, DEVICES AND METHODS FOR THE CONTROL IN VITRO OF CHEMICAL MICROAMBIENT IN CELL CULTURES

The present invention relates to compositions comprising a polymeric matrix or a gel containing functional enzymes capable of re-creating under culture conditions the cell microenvironment existing in vivo. The present invention also relates to devices for cell cultures comprising such compositions, in particular hydrogel and the use thereof to control the chemical microenvironment of a cell culture or mimic physiological or pathological conditions of the in vivo cells. The compositions and the devices described herein could be also used in vitro for evaluating the therapeutic effect of a compound on a determined cell line or on primary cells. 1. A composition for use in in vitro cell cultures comprising a matrix that contains one or more enzymes capable of catalyzing the reduction of oxygen , in which the oxygen gradient in said cell cultures is controlled.2. The composition according to wherein said matrix is a polymeric or protein matrix.3. The composition according to wherein said enzyme is selected from Glucose oxidase (GOx) claim 1 , NADPH oxidase claim 1 , xanthine oxidase claim 1 , lactate oxidase claim 1 , cytochrome oxidase or Laccases.4. The composition according to further comprising the Catalase enzyme.5. The composition according to wherein said enzymes are covalently bound to said matrix by a cross-linking agent (cross-linker).6. The composition according to wherein said crosslinking agent is glutaraldehyde (GDA) claim 5 , Bis(sulfosuccinimidil) suberate claim 5 , N-hydroxysuccinimide claim 5 , formaldehyde claim 5 , or photoreactive agents.7. The composition according to wherein said enzymes are trapped in a polymeric matrix through mechanical and/or electrostatic interactions.8. The composition according to wherein said composition is a hydrogel.9. The composition according to wherein said hydrogel is silicone hydrogel claim 8 , polyacrylamides claim 8 , cellulose claim 8 , cellulose derivatives claim 8 , collagen claim 8 , carboxymethylcellulose ...

IMMUNOISOLATION DEVICE

The purpose of the present invention is to solve conventional problems involving immunoisolation devices using a porous membrane as an immunoisolation membrane, such problems including fibrotic formation on the device surface, shortage of oxygen supplied to cells, and non-uniform distribution of cells. An immunoisolation device for transplantation is characterized by comprising: an immunoisolation membrane; and an oxygen supply mechanism and a three-dimensional cell support which are tightly enclosed inside the immunoisolation membrane by physical sealing, a biocompatible adhesive, or a combination thereof, wherein the immunoisolation membrane is a porous membrane provided with a hydrophilic layer on the outer surface, and the oxygen supply mechanism can supply oxygen to cells supported by the three-dimensional cell support through an oxygen permeable membrane. 1. An immunoisolation device for transplantation , comprising:an immunoisolation membrane; and an oxygen supply mechanism and a three-dimensional cell support which are tightly enclosed inside the immunoisolation membrane by physical sealing, a biocompatible adhesive, or a combination thereof, in whichthe immunoisolation membrane is a porous membrane provided with a hydrophilic layer on the outer surface, andthe oxygen supply mechanism can supply oxygen to a cell supported by the three-dimensional cell support through an oxygen permeable membrane.2. The immunoisolation device according to claim 1 , wherein the porous membrane is a fluororesin porous membrane.3. The immunoisolation device according to claim 2 , wherein the hydrophilic layer is a layer containing a hydrophilic compound physically adsorbed or fixed on the surface of the porous membrane.4. The immunoisolation device according to claim 2 , wherein the hydrophilic layer is a layer containing a hydrophilic compound introduced on the surface of the porous membrane via a covalent bond.5. The immunoisolation device according to claim 3 , wherein the ...

Small Enzyme Particles For Interesterification

The invention provides enzyme particles comprising an immobilized lipolytic enzyme, a siliceous material, an organic filter aid, and a water-soluble polyol selected from carbohydrates and sugar alcohols. The particles are suitable for enzymatic interesterification of triglycerides, and subsequent separation of the enzyme and triglycerides by filtration. 1. A plurality of enzyme particles , wherein said particles comprise a lipolytic enzyme , a siliceous material , an organic filter aid , and a water-soluble polyol selected from carbohydrates and sugar alcohols.2. The particles of claim 1 , which comprise the siliceous material in an amount of 10-80% w/w.3. The particles of claim 1 , which comprise the organic filter aid in an amount of 10-80% w/w.4. The particles of claim 1 , which comprise the siliceous material and the organic filter aid in a total amount of 40-95% w/w.5. The particles of claim 1 , which comprise the polyol in an amount of 2-50% w/w.6. The particles of claim 1 , which comprise the lipolytic enzyme in an amount of 1-50% w/w.7. The particles of claim 1 , wherein the siliceous material is silica.8. The particles of claim 1 , wherein the organic filter aid is a water-insoluble polysaccharide.9. The particles of claim 1 , wherein the organic filter aid is cellulose.10. The particles of claim 1 , wherein the polyol is selected from the group consisting of dextrin claim 1 , maltodextrin claim 1 , trisaccharides claim 1 , disaccharides claim 1 , monosaccharides claim 1 , and mixtures thereof.11. The particles of claim 1 , wherein the polyol is maltodextrin having a DE between 6 and 52.12. The particles of claim 1 , wherein the lipolytic enzyme is a lipase.13. The particles of claim 1 , which is a substantially homogenous composition of the ingredients claim 1 , prepared by spray drying or absorption claim 1 , followed by drying.14. The particles of claim 1 , which has an average diameter below 100 μm.15. A powder or a slurry/suspension comprising the ...

餐厨垃圾处理的固定化微生物菌剂的制备方法及应用

本发明公开了一种餐厨垃圾处理的固定化微生物菌剂的制备方法及应用，该方法包括：分别对克鲁维毕赤酵母和林生地霉进行高密度液体发酵，再将菌液按比例混合；将复合菌液浓缩后，加入至改性后的载体中，进行混合，并在混合过程中加入交联剂，然后进行恒温通气培养；恒温通气培养后，进行低温干燥，得到固定化微生物菌剂。本发明方法不仅显著提高了载体固定的菌体量，还使载体与菌体之间的结合紧密，防止菌体在制备过程和保藏中的活菌大量损失，提高了单位质量菌剂的活菌数，减少了菌剂的使用量；制备获得的解餐厨垃圾能力强、能够消除降解餐厨垃圾过程中产生的异味，而且载体中固定的菌体量大，载体与菌体之间的结合更紧密，活菌生物量大。

餐厨垃圾处理的无臭型改良微生物菌剂及其制备方法和应用

本发明公开了一种餐厨垃圾处理的无臭型改良微生物菌剂及其制备方法和应用，该菌剂的组分为复合菌体5～15份；载体50～150份；粘合剂1～3份；保护剂1～5份；复合菌体由质量比为1～15:0.5～10的克鲁维毕赤酵母(Pichia kluyveri)ZJB‑091和林生地霉(Geotrichum silvicola)ZJB‑092混合而成；载体为小麦秸秆。本发明采用克鲁维毕赤酵母和林生地霉作为复合菌体，小麦秸秆作为载体，获得的菌剂不仅解餐厨垃圾能力显著增强，而且能够消除降解餐厨垃圾过程中产生的异味，并防止菌剂结块，进而避免菌剂使用过程中因机械粉碎而造成的活菌数降低的问题。

Method for producing ficin preparation in gel based on carboxymethylcellulose

FIELD: biotechnology.SUBSTANCE: invention relates to biotechnology. A method is proposed for obtaining a heterogeneous preparation in a gel based on ficin and carboxymethyl cellulose, including the immobilization of an enzyme preparation with an enzyme concentration of 3 mg/ml solution in 0.05 Μ borate buffer with 0.1 Μ KCl in the composition, pH 9.0 in a ratio of 20 ml of the enzyme solution per 1 g of sodium salt carboxymethyl cellulose with a molecular weight of ~ 90 kDa; incubation at room temperature with occasional stirring, and washing with 0.05 Μ Tris-HCl buffer pH 7.5 until there is no protein in the washings.EFFECT: invention provides an extension of the arsenal of carriers for obtaining immobilized ficin, which has greater activity and provides enzymatic reactions in solution and on a solid substrate.1 cl, 3 dwg, 1 ex РОССИЙСКАЯ ФЕДЕРАЦИЯ (19) RU (11) (13) 2 771 183 C1 (51) МПК C12N 11/04 (2006.01) C12N 11/12 (2006.01) A61K 38/46 (2006.01) ФЕДЕРАЛЬНАЯ СЛУЖБА ПО ИНТЕЛЛЕКТУАЛЬНОЙ СОБСТВЕННОСТИ (12) ОПИСАНИЕ ИЗОБРЕТЕНИЯ К ПАТЕНТУ (52) СПК C12N 11/04 (2022.02); C12N 11/12 (2022.02); A61K 38/46 (2022.02) (21)(22) Заявка: 2021124260, 13.08.2021 (24) Дата начала отсчета срока действия патента: Дата регистрации: Приоритет(ы): (22) Дата подачи заявки: 13.08.2021 (45) Опубликовано: 28.04.2022 Бюл. № 13 C 1 2 7 7 1 1 8 3 R U (54) СПОСОБ ПОЛУЧЕНИЯ ПРЕПАРАТА ФИЦИНА В ГЕЛЕ НА ОСНОВЕ КАРБОКСИМЕТИЛЦЕЛЛЮЛОЗЫ (57) Реферат: Изобретение относится к биотехнологии. при комнатной температуре с периодическим Предложен способ получения гетерогенного перемешиванием и промывку 0.05 Μ трис-HCl препарата в геле на основе фицина и буфером с рН 7.5 до отсутствия в промывных карбоксиметилцеллюлозы, включающий водах белка. Изобретение обеспечивает иммобилизацию ферментного препарата с расширение арсенала носителей для получения концентрацией фермента 3 мг/мл раствора в 0.05 иммобилизованного фицина, обладающего Μ боратном буфере с 0.1 Μ KCl в составе, рН 9.0 большей активностью и ...

Normal-temperature aerobic treatment method for perishable garbage

本发明提供易腐垃圾的常温好氧处理方法，涉及环保技术领域，包括提供易腐垃圾，送入生物降解仓中；提供固定化微生物菌剂对易腐垃圾进行生物降解，以及，将生物降解仓排出的物料进行包括固液分离和污水处理的后处理工序；上述固定化微生物菌剂的制备方法包括：提供好氧菌的菌悬液；并将好氧菌经固定化处理，固载于载体中，制得固定化填料；以及，提供海藻酸钠‑CaCl 2 体系对固定化填料进行成型处理，制得微生物菌剂。本发明提供的常温好氧处理方法终产物是二氧化碳和水，生物降解效果好，减量率高，实现易腐垃圾的无害化生物减量；菌剂制备方法能增加菌剂的机械强度，提升耐冲击负荷能力，提高菌剂的耐酸碱性，增强菌剂稳定性和可回收性。

Method for preparing immobilized mycelium of basidiomycete fomitopsis officinalis

FIELD: biotechnology. SUBSTANCE: invention relates to biotechnology and can be used to produce immobilized mycelium of basidium fomitopsis officinalis. Method involves cultivating Fomitopsis officinalis VKPM F-961 strain together with a producer strain of bacterial cellulose Gluconacetobacter hansenii VKPM V-10547 on a synthetic medium containing glucose, yeast extract, Na 2 HPO 4 , K 2 HPO 4 , (NH 4 ) 2 SO 4 , monohydrate of citric acid, medium pH 6.5. EFFECT: invention provides high output and productivity of mycelium. 1 cl, 2 dwg, 2 tbl, 2 ex РОССИЙСКАЯ ФЕДЕРАЦИЯ (19) RU (11) (13) 2 707 541 C2 (51) МПК C12N 1/14 (2006.01) C12N 11/12 (2006.01) ФЕДЕРАЛЬНАЯ СЛУЖБА ПО ИНТЕЛЛЕКТУАЛЬНОЙ СОБСТВЕННОСТИ (12) ОПИСАНИЕ ИЗОБРЕТЕНИЯ К ПАТЕНТУ (52) СПК C12N 1/14 (2019.05); C12N 11/12 (2019.05) (21)(22) Заявка: 2017145642, 25.12.2017 (24) Дата начала отсчета срока действия патента: Дата регистрации: 27.11.2019 (43) Дата публикации заявки: 25.06.2019 Бюл. № 18 (45) Опубликовано: 27.11.2019 Бюл. № 33 Адрес для переписки: 119991, Москва, ул. Трубецкая, 8, стр. 2, ФГАОУ ВО Первый МГМУ им. И.М. Сеченова, технопарк 2 7 0 7 5 4 1 C 2 (56) Список документов, цитированных в отчете о поиске: RU 2330676 С1, 10.08.2008. RU 2464307 С1, 20.10.2012. RU 2360960 С1, 10.07.2009. КИСЕЛЕВА О.В. и др. Глубинное культивирование серно-желтого трутовика с целью получения белковой биомассы. Химия растительного сырья, 2011, т. 4. c. 337-338. (54) СПОСОБ ПОЛУЧЕНИЯ ИММОБИЛИЗОВАННОГО МИЦЕЛИЯ БАЗИДИОМИЦЕТА FOMITOPSIS OFFICINALIS (57) Реферат: Изобретение относится к биотехнологии и целлюлозы Gluconacetobacter hansenii ВКПМ Вможет быть использовано для получения 10547 на синтетической среде, содержащей иммобилизованного мицелия базидиального глюкозу, дрожжевой экстракт, Na2HPO4, K2HPO4, гриба Fomitopsis officinalis. Способ (NH4)2SO4, моногидрат лимонной кислоты, рН предусматривает культивирование штамма среды 6,5. Изобретение обеспечивает повышение Fomitopsis officinalis ВКПМ F-961 совместно со выхода и ...

METHOD OF OBTAINING IMMOBILIZED MICELIUM BY BIZIDIOMYCETET FOMITOPSISOFFICINALIS

РОССИЙСКАЯ ФЕДЕРАЦИЯ (19) RU (11) (13) 2017 145 642 A (51) МПК C12N 1/20 (2006.01) ФЕДЕРАЛЬНАЯ СЛУЖБА ПО ИНТЕЛЛЕКТУАЛЬНОЙ СОБСТВЕННОСТИ (12) ЗАЯВКА НА ИЗОБРЕТЕНИЕ (21)(22) Заявка: 2017145642, 25.12.2017 Приоритет(ы): (22) Дата подачи заявки: 25.12.2017 (43) Дата публикации заявки: 25.06.2019 Бюл. № 18 A R U (57) Формула изобретения Способ получения иммобилизованного мицелия базидиального гриба Fomitopsis officinalis (Vill.:Fr.) Bond. EtSing, заключающийся в культивировании штамма Fomitopsis officinalis совместно со штаммом-продуцентом бактериальной целлюлозы Gluconacetobacter hansenii на синтетической среде состава, г/л: глюкоза - 7.0, дрожжевой экстракт - 5.0, Na2HPO4 - 0.27, К2НРО4 - 0.2, (NH4)2SO4 - 0.3, моногидрат лимонной кислоты - 0.115, рН среды 6,5. Стр.: 1 A 2 0 1 7 1 4 5 6 4 2 (54) СПОСОБ ПОЛУЧЕНИЯ ИММОБИЛИЗОВАННОГО МИЦЕЛИЯ БИЗИДИОМИЦЕТА FOMITOPSISOFFICINALIS 2 0 1 7 1 4 5 6 4 2 (72) Автор(ы): Громовых Татьяна Ильинична (RU), Луценко Сергей Викторович (RU), Айрапетова Ася Юрьевна (RU), Фельдман Наталия Борисовна (RU), Садыкова Вера Сергеевна (RU), Гаврюшина Ирина Александровна (RU), Каширин Владимир Валентинович (RU) R U Адрес для переписки: 119991, Москва, ул. Трубецкая, 8, стр. 2, ФГАОУ ВО Первый МГМУ им. И.М. Сеченова, технопарк (71) Заявитель(и): федеральное государственное автономное образовательное учреждение высшего образования Первый Московский государственный медицинский университет имени И.М. Сеченова Министерства здравоохранения Российской Федерации (Сеченовский университет) (RU)

Three-layered membrane for use in an electrochemical sensor system

A system for electrochemical measurement of glucose concentration in an undiluted test sample, e.g. blood is provided containing a sensor including a three-layered contiguous membrane. The membrane has a thickness of 50 to 130 microns, and is composed of a 1 to 10 micron thick first layer, a 10 to 30 micron thick second layer having an average pore diameter of 15 nanometers and a 40 to 80 micron thick third layer containing glucose oxidase. The third layer is less dense than the first and second layers and the first layer is more dense than the second layer. The layers of the membrane are fused together such that no clear distinction can be made between the layers at the boundary. The sensor is calibrated in a standard glucose solution which includes catalase as a hydrogen peroxide scavenger, and the sensor has a response that is linear throughout the concentration range of glucose in an undiluted sample. In one embodiment, the system is a polarographic cell structure containing an electrically insulating receptacle, an electrode mounted in the receptacle, and the three-layered membrane.

ENZYME LAVASTATIN ESTErase, IMMOBILIZED ON A SOLID CARRIER, METHOD FOR IMMOBILIZING AN ENZYME, APPLICATION OF AN IMMOBILIZED ENZYME, BIO-CATALYZED CIRCUIT AND SUSCEPTIVELY

1. Фермент ловастатин эстераза, иммобилизованный на водонерастворимом твердом носителе, отличающийся тем, что фермент ковалентно связан с твердым носителем, активированным по меньшей мере бифункциональным связывающим агентом, причем соединение твердого носителя и по меньшей мере бифункционального связывающего агента является таким, что иммобилизованная ловастатин эстераза проявляет по меньшей мере в 5 раз большую гидролитическую активность к ловастатину и солям ловастатина, в присутствии симвастатина и/или солей симвастатина, чем к симвастатину и солям симвастатина. ! 2. Фермент ловастатин эстераза по п.1, отличающийся тем, что твердый носитель представляет собой модифицированный полисахарид, представляющий собой ди-(С1-6алкил)амино-С1-6алкилцеллюлозу, и по меньшей мере бифункциональный агент, активирующий твердый носитель, представляет собой O-сульфонат циануровой кислоты или галогенангидрид циануровой кислоты. ! 3. Фермент ловастатин эстераза по п.1, отличающийся тем, что твердый носитель представляет собой модифицированный силикагель, предпочтительно модифицированный амино-С1-6алкил-три(С1-6алкокси)силаном, и по меньшей мере бифункциональный агент, активирующий твердый носитель, представляет собой O-сульфонат циануровой кислоты или галогенангидрид циануровой кислоты. ! 4. Фермент ловастатин эстераза по любому из пп.2 или 3, отличающийся тем, что по меньшей мере бифункциональный агент, активирующий твердый носитель, представляет собой цианурхлорид. ! 5. Фермент ловастатин эстераза по п.1, отличающийся тем, что твердый носитель представляет собой полигалактозид, а по меньшей мере бифункциональный агент, � РОССИЙСКАЯ ФЕДЕРАЦИЯ (19) RU (11) 2010 122 385 (13) A (51) МПК C12M 1/40 (2006.01) ФЕДЕРАЛЬНАЯ СЛУЖБА ПО ИНТЕЛЛЕКТУАЛЬНОЙ СОБСТВЕННОСТИ (12) ЗАЯВКА НА ИЗОБРЕТЕНИЕ (71) Заявитель(и): ИНИСТИТУТ ХЕМИ ОРГАНИЧНЕЙ, ПОЛЬСКАЯ АКАДЕМИЯ НАУК (PL) (21)(22) Заявка: 2010122385/10, 18.11.2008 Приоритет(ы): (30) Конвенционный приоритет: 19.11.2007 PL P383819 (87) Публикация ...

Embedding and curing method of D-pantolactone hydrolase

本发明涉及一种D‑泛解酸内酯水解酶包埋固化方法，其方法是将以尖镰孢霉菌发酵生产的D‑泛解酸内酯水解酶发酵液先用陶瓷膜微滤，所得菌丝体浓缩液升温至45～55℃，加入改性淀粉醚，搅拌至分散均匀，静置，之后依次加入硅藻土和羧甲基纤维素钠，搅拌均匀至无气泡后，降温至2～5℃，制粒，减压干燥得到D‑泛解酸内酯水解酶菌丝包埋固化物。本发明采用材料易得，质量可控，形成固化酶效果可靠，酶活力回收率达64%以上，方法简便，可多次回收利用，生产成本小，本发明废水量少，减轻环保处理强度,适合工业化生产。

A method of preparing a substrate for immobilization of functional substances thereon and the substrate obtained therefrom

CONTINUOUS METHOD FOR THE ENZYMATIC PRODUCTION OF ISOMALTULOSE

Method and carrier for the production of isomaltulose by immobilized microorganisms

Ferment lovastatin esterase, immobilised on hard carrier, method of ferment immobilisation, biocatalysed flow reactor and method of simvastatin treatment

FIELD: biotechnologies. SUBSTANCE: ferment lovastatin esterase is proposed, which is immobilised on a water-insoluble hard carrier activated with a bifunctional agent. At the same time the hard carrier represents a modified di-(C 1-6 alkyl)amino-C 1-6 alkylcellulose, in another version the hard carrier represents a silica gel modified with amino-C 1-6 alkyl-tri(C 1-6 alkoxy)silane, and the bifunctional agent that activates the hard carrier represents O-sulfonate of cyanuric acid or acid halide of cyanuric acid. In the third version the hard carrier represents agarose, and the bifunctional agent is a compound that corresponds to the formula , as defined in the formula. Methods (versions) are proposed to immobilise the ferment lovastatin esterase on specified water-insoluble hard carriers. According to the methods, in process of mechanical mixing the bifunctional activating agent is brought in contact with the hard carrier in the dissolvent. The activated hard carrier is separated by filtration, then dried and suspended in a water mixture, containing the ferment lovastatin esterase, with performance of ferment immobilisation. The suspended substance is separated by filtration, washed with the buffer solution and dried. Also the method is proposed to treat simvastatin, including treatment of the simvastatin salt solution, containing the remaining amount of the lovastatin salt, immobilised witht the ferment lovastatin esterase, and a biocatalysed flow reactor is proposed with a layer for realisation of this method. The reactor comprises a reactor body (1) with the inner space (2), connected with a liquid inlet (3) and connected with a liquid outlet (4), in the inner space there is a perforated plate supporting the layer (5), containing the ferment lovastatin esterase, immobilised on the water-insoluble hard carrier. EFFECT: immobilised lovastatin esterase according to the invention demonstrates at least 5 times higher hydrolytic activity in respect to lovastatin and ...

The method of obtaining the enzyme, fixed on a solid carrier

Material for cell cultures derived from plants

Предложен материал для культивирования или доставки эукариотических клеток. Материал содержит происходящие из растений механически дезинтегрированные целлюлозные нановолокна и/или их производные в форме гидрогеля или мембраны во влажном состоянии. Диаметр целлюлозных нановолокон или пучков нановолокон в целлюлозных нановолокнах и/или их производных меньше 1 мкм, предпочтительно - меньше 200 нм, более предпочтительно - меньше 100 нм. Данный материал получают путем смешивания происходящих от растений механически дезинтегрированных целлюлозных нановолокон и/или их производных с водой. Предложен также матрикс для культивирования или доставки клеток, который содержит живые клетки и указанный материал, образующий гидрогель, при этом клетки находятся в этом матриксе в виде трехмерной или двухмерной структуры. Для культивирования клеток осуществляют контакт полученных клеток с материалом для образования матрикса, в котором культивирование клеток протекает в виде трехмерной или двухмерной структуры. Предложено также применение указанного материала для лабораторных и/или промышленных целей в качестве среды или компонента среды для сохранения клеток in vitro. Группа изобретений обеспечивает стабильность клеток в матриксе, а также гомогенное распределение их в указанном матриксе. 6 н. и 16 з.п. ф-лы, 15 ил., 11 пр. РОССИЙСКАЯ ФЕДЕРАЦИЯ (19) RU (11) (13) 2 597 978 C2 (51) МПК C12N 5/07 (2010.01) C12N 5/0735 (2010.01) C08L 1/02 (2006.01) C08J 3/075 (2006.01) A61K 47/38 (2006.01) ФЕДЕРАЛЬНАЯ СЛУЖБА ПО ИНТЕЛЛЕКТУАЛЬНОЙ СОБСТВЕННОСТИ (12) ОПИСАНИЕ (21)(22) Заявка: ИЗОБРЕТЕНИЯ К ПАТЕНТУ 2013122757/10, 26.10.2011 (24) Дата начала отсчета срока действия патента: 26.10.2011 Приоритет(ы): (30) Конвенционный приоритет: (43) Дата публикации заявки: 10.12.2014 Бюл. № 34 (73) Патентообладатель(и): ЮПМ-КИММЕНЕ КОРПОРЭЙШН (FI) R U 27.10.2010 FI 20106121 (72) Автор(ы): ИЛИПЕРТТУЛА Марьо (FI), ЛОРАН Патрик (FI), БХАТТАЧАРЬЯ Мадхушри (FI), ЛОУ Яньжу (FI), ЛАУККАНЕН Антти (FI) (56) Список ...

Matrix and composition for microbial cultivation of gram-positive bacteria

PROCESS FOR PREPARING POROUS CELLULOSE PEARLS

Porous cellulose beads are prepared by distributing droplets of a solvent mixture containing a cellulose derivative into a precipitating solution to form porous beads which are then washed and hydrolyzed to form porous cellulose beads. The porous cellulose beads, which may be cross-linked, if desired, by suitable treatment, are useful carriers to which enzymes can be immobilized. The beads may also be used for the separation of enzymes, proteins, nucleic acids and the like, or to remove metal ions from dilute mining solutions.

Polynucleotide phosphorylase prodn for polynucleotide prodn

The enzyme (PNPase) is obtd. by (a) suspending a cell mass separated from a Micrococcus lacteus fermentation medium in aqs. saline soln., and homogenising or lysing the suspension to rupture the cells to give a suspension of insol. cell debris in an aqs. PNPase soln.; (b) adding (NH4)2SO4 to a concn. of approx. 24% and centrifuging to remove the cell debris and some protein material; (c) adding to the supernatant of aqs. PNPase sufficient lower alkanol to ppt. almost all of the PNPase, and centrifuging the aqs. alcoholic mixt. to recover the ppted. enzyme. PNPase catalyses the polymerisation of nucleoside diphosphates to polynucleotides. For use as a catalyst, the enzyme is covalently bound to activated cellulose.

IMMOBILIZED ENZYMES, THEIR PREPARATION AND THEIR USE.

L'invention concerne des enzymes immobilisées par adsorption sur des supports d'enzymes solides, les enzymes étant immobilisées par adsorption sur des particules de substances naturelles non modifiées présentant essentiellement un caractère cellulosique. The invention relates to enzymes immobilized by adsorption on solid enzyme carriers, the enzymes being immobilized by adsorption on particles of unmodified natural substances essentially having a cellulosic character.

Patent FR2348942B3

Porous cellulose beads are prepared by distributing droplets of a solvent mixture containing a cellulose derivative into a precipitating solution to form porous beads which are then washed and hydrolyzed to form porous cellulose beads. The porous cellulose beads, which may be cross-linked, if desired, by suitable treatment, are useful carriers to which enzymes can be immobilized. The beads may also be used for the separation of enzymes, proteins, nucleic acids and the like, or to remove metal ions from dilute mining solutions.

Enzyme-cellulose complexes - esp. suitable for milk coagulation for cheeses, utilisable as large particles in enzymatic fixed or mobile beds

The complex is prepd. by dispersing the cellulose deriv. in an aqs. soln. of the enzymen, esp. a protease, and particularly rennet, in the presence of a buffer maintaining the pH of the soln. at 5-10, esp. 6-9, and effecting contact between the two at 0-45 degrees C for a time sufficient for the enzyme to become fixed onto the cellulose, i.e. 24-48 hrs. The amt. of enzyme used is 0.1-100%, esp. 1-60% by wt. of the cellulose used. The cellulose deriv. is obtd. by chlorinating or sulphochlorinating an alkali cellulose in an org. liq. which is not destroyed by the chlorinating agent, e.g. pyridine or dioxane.

PROCESS FOR THE ISOMERIZATION OF GLUCOSE

PROTEOLYTIC COATING FOR WOUNDS AND PROCESS FOR PREPARING THE SAME

REVETEMENT PROTEOLYTIQUE POUR PLAIES ET PROCEDE POUR SA PREPARATION; LE REVETEMENT EST CONSTITUE DE CELLULOSE SPHERIQUE POREUSE AYANT UNE TAILLE DES PARTICULES DE 0,05 A 0,5MM CONTENANT UNE PROTEASE IMMOBILISEE QUE L'ON PREPARE A PARTIR DE PERLES DE CELLULOSE REGENEREE GONFLEES DANS L'EAU, N'AYANT JAMAIS ETE PREALABLEMENT SECHEES QUE L'ON DEBARRASSE DES IMPURETES TOXIQUES, QUE L'ON MODIFIE PAR IMMOBILISATION DE PROTEASE ET QU'ON LAVE AVEC DES TAMPONS PUIS QU'ON SECHE DE PREFERENCE PAR LYOPHILISATION.

PRODUCT FOR ENVIRONMENTAL BIOTRATING

Method of producing polymer composition

Fibrous ion exchange cellulose composites are prepared by agglomerating a hydrophobic polymer and fibrous cellulose and then derivatizing the cellulose to impart ion exchange properties thereto.

Patent FR2206329B1

Chemical processing cell with nanostructured membranes

A chemical processing cell includes an upstream membrane and a downstream membrane. The upstream membrane generates a first reaction product. The downstream membrane converts the first reaction product to a second reaction product.

PRODUCT FOR ENVIRONMENTAL BIOTRATING

L'invention concerne un produit biodégradable pour le biotraitement environnemental se composant d'une enveloppe de conditionnement perméable à l'oxygène, réalisée au moyen d'une composition biodégradable extrudable, comprenant au moins un matériau végétal broyé fibreux, au moins un agent végétal non fibreux intervenant comme liant de ce matériau, et au moins un agent plastifiant, d'un fourrage interne formulé, réalisée avec au moins un acide aminé agissant comme membrane osmotique et comme milieu nourricier, d'un volume aqueux d'une culture contenant bactéries, fongi et/ou levures aérobies placées à l'intérieur de ladite enveloppe de conditionnement.L'invention concerne également le procédé de réalisation du produit et ses applications au biotraitement de sols, d'eaux polluées, de traitement des graisses, de compostage et de traitement des milieux ambiants en général.

Biosensor for detection of and distinguishing between cholinesterases inhibitors

The invention relates to a biosensor of cholinesterase inhibitors in air, water, foodstuffs, soil, at surfaces and in extracts from samples, comprised of cellulose textile with bioafinity immobilized and stabilized cholinesterase, colour standard and of a carrier from substrate and chromogenic agent and its application to the detection of and distinguishing between cholinesterase in-hibitors. In distinguishing between the inhibitors by a biosensor in case of cholinesterase immobilized at cellulose textile of the biosensor after inhibition by organophosphorous or carbamate inhibitor, hydrolytic activity restores the activity of nucleophilic agents. According to the level of the restored hydrolytic activity of the immobilized cholinesterase in relation to the exposition time to reactivators, e.g. to quaternary aldoximes or water, and according to the activity change of the immobilized cholinesterase exposed in the inhibitor solution and in the inhibitor solution containing ions, e.g. natrium, calcium or ammonium ions, the type of the organophosphorous and carbamate inhibitor is determined.

PRODUCT FOR ENVIRONMENTAL BIOTRATING

Patent FR2234311B1

Cells contg. glucose-isomerase enclosed in cellulose ester - with magnesium cpd. gives compsn. of extended life for isomerisation of glucose to fructose

Cellulose ester, pref. diacetate or diacetate/triacetate mixt., platelets or filaments which enclose cells of a microorganism contg. glucose-isomerase contain >=1 Mg cpd. which is slighty soluble in water. They can also contain a slightly soluble Co cpd. The microorganism can be Aerobacter cloacae, Arthrobacter, Bacillus coagulans, Lactobacillus brevis, Streptomyces sp. (phaeochromogenes, echinatus, flavovirens, achromogenes, albus, olivaceus, livochromogenes, violaceoniger, bikiniensis, venezuelae) or Actinoplanes missouriensis. The Mg cpd. is pref. the oxide, phosphate, basic carbonate, or their mixts. The Co cpd. is pref. the hydroxide, carbonate, orthophosphate or oxalate of CoII. The Co cpd. is present at up to 50% of their combined wt. Alternatively, Mg++ or Mg++/Co++ ions can be introduced by means of a dried and ground cation exchange resin carrying sulphonic gps. The compsn. has an extended life compared with previously used compsns. Also, little or no Mg++ ions need be added to the glucose syrup itself, and no Co++ ions. The Mg++ concn. in the fructose syrup obtd. is not >10 mg/l which is completely satisfactory in foodstuffs.

IMPROVED PROCESS FOR THE PREPARATION OF AGGLOMERATED FIBROUS CELLULOSE AND FIBROUS CELLULOSE AND HYDROPHOBIC POLYMER AGGLOMERATE OBTAINED BY SAID PROCESS

PROCESS FOR PREPARING POROUS CELLULOSE PEARLS

Microbial slow-release treatment method for organic wastewater

提供一种有机废水的微生物缓释处理法，采用吸附‑包埋法协同制备得到的微生物处理剂，能够高效处理高浓度有机废水，具有稳定可控，净化效率高，并且同时制氢产量高的特点。

Biodegradable immobilized enzymes and methods of making the same

The present application discloses immobilized enzymes and immobilized enzyme materials comprising a crosslinked enzyme having a support material which includes a biomass material different than the biomass used to initially derive the enzyme. Optionally, the immobilized enzyme further includes a polymeric material and/or the biomass which was used to initially derive the enzyme. The resulting immobilized enzyme materials may be biodegradable. The present application also discloses methods of making and using the disclosed immobilized enzyme materials.

Cell culture material

Esillä oleva keksintö kohdistuu materiaaliin, jota voidaan käyttää solujen viljelyyn ja siirtoon sekä lääkeaineiden kuljetukseen. Tämä materiaali sisältää selluloosan nanokuituja tai niiden johdannaista, jotka selluloosan nanokuidut ovat hydrogeelina tai kalvona. Keksinnössä saadaan myös aikaan menetelmät näiden materiaalien ja koostumusten tuottamiseksi ja niiden käytöt.

Biodegradable immobilized enzymes and methods of making the same

本发明公开了一种固定化酶和包括一种具有支撑材料的交联酶的固定化酶材料，所述支撑材料包括不同于最初所述酶来源的生物质的生物质材料。优选的，所述固定化酶还包括一种聚合材料和/或最初取得所述酶的生物质，所得到的固定化酶材料可以是可生物降解的。本发明还公开了制备和使用所公开的固定化酶材料的方法。

Patent JPS5046890A

Immobilized enzymes on a solid carrier

Enzymes immobilisees sur un support solide insoluble en milieu aqueux. Un support contenant de la cellulose et de la lignine est traite par un agent oxydant, puis mis en contact avec une diamine. Apres stabilisation de la liaison amine-support puis activation des groupes amines, l'enzyme est couplee avec le support active. Les derives enzymatiques obtenus sont utilises pour traiter des jus sucres.

Patent FR2472015B1

Agglomerated fibrous ion exchange cellulose

An agglomerated fibrous ion exchange cellulose composite wherein the cellulose is embedded in a hydrophobic polymer and relatively large portions of the cellulose is free to adsorb charged macro-molecules.

Improved galactose isomerases and use thereof in the production of tagatose

The present invention discloses genes encoding D-galactose isomerase, D- galactose isomerases expressed from the said genes, a recombinant expression vector comprising the a gene encoding D-galactose isomerase, a microorganism transformed with the said expression vector, a process for preparing D-galactose isomerase from the transformed microorganism and a process for preparing D-tagatose using an D- galactose isomerase of the invention.

COMPOSITION OF TONE EXCHANGER FIBROUS AGGLOMERATED CELLULOSIS AND PROCESS FOR ITS PREPARATION

Method for degrading malachite green by using bacterial cellulose membrane-immobilized phanerochaete chrysosporium

本发明公开了一种细菌纤维素膜固定化黄孢原毛平革菌用于孔雀石绿降解的方法，包括以下步骤：配制固体平板培养基，得到活化的木醋杆菌Acetobacter xylinum NUST4.2作为菌种，静态发酵培养得到细菌纤维素膜载体，裁剪得到不同大小的长方体小块；制备黄孢原毛平革菌的孢子悬浮液；配制液体培养基；将不同大小、数目的细菌纤维素膜载体加入液体培养基，并将黄孢原毛平革菌的孢子悬浮液接至该液体培养基，制备固定化黄孢原毛平革菌；采用得到的固定化黄孢原毛平革菌，对孔雀石绿染料废水进行脱色试验，确定细菌纤维素膜载体对固定化黄孢原毛平革菌降解孔雀石绿染料废水效果的影响。本发明提高了黄孢原毛平革菌对孔雀石绿的降解效率及稳定性。

Process for the preparation of copolymers of polysaccharides as supports of enzymatic activity and copolymers thus obtained

PROCESS FOR THE PREPARATION OF COPOLYMERS OF POLYSACCHARIDES AS SUPPORTS OF ENZYMATIC ACTIVITY AND COPOLYMERS THUS OBTAINED ABSTRACT OF THE DISCLOSURE A process for the preparation of a copolymer of a polysaccharide having enzymatic activity is described which comprises adding to a suspension of a polysaccharide in an aqueous medium, a vinyl monomer and an enzyme, then a catalyst which comprises a metallic salt and irradiating the resulting mixture with a source of ultravlolet light. The metallic salt is preferably a ferric salt. The vinyl monomer may be methylacrylate, glycidylmethacrylate, acrylonitrile, bis-acryloylpiperazlne or symmetrical N,N',N"-trisacryloyl-hexahydrotriazine. The process is applicable to a great variety of enzymes such as proteolytic enzymes, hydrolytic enzymes, oxidases, dehydrogenases and isomerases.

Biodegradable product, useful e.g. for environmental biotreatment and to treat bioincrease of industrial wastewater in situation of accidental toxicity, comprises conditioning layer, internal coating and an aqueous volume of culture

L'invention concerne un produit biodégradable pour le biotraitement environnemental se composant d'une enveloppe de conditionnement perméable à l'oxygène, réalisée au moyen d'une composition biodégradable extrudable, comprenant au moins un matériau végétal broyé fibreux, au moins un agent végétal non fibreux intervenant comme liant de ce matériau, et au moins un agent plastifiant, d'un revêtement interne formulé, déposé sur la surface de l'enveloppe de conditionnement, réalisée avec au moins un acide aminé, ce revêtement agissant comme membrane osmotique et comme milieu nourricier, d'un volume aqueux d'une culture contenant bactéries, fongi et/ou levures aérobies placés à l'intérieur de ladite enveloppe de conditionnement.L'invention concerne également le procédé de réalisation du produit et ses applications au biotraitement de sols, d'eaux polluées, de compostage et des milieux ambiants en général.

Immobilised lysine decarboxylase, its preparation, 1,5 pentanediamine preparation methods and product

本发明涉及固定化赖氨酸脱羧酶、其制备及1,5‑戊二胺制备方法及制得的1,5‑戊二胺。具体涉及一种包括醛基修饰的高分子载体和赖氨酸脱羧酶的固定化赖氨酸脱羧酶，及以醛基修饰的高分子载体制备固定化赖氨酸脱羧酶的方法；及以固定化赖氨酸脱羧酶制备1,5‑戊二胺的方法及其制得的1,5‑戊二胺。该固定化方法对酶的固定化效率高，使用稳定性好，提高酶的使用效率，解决传统工艺中游离态赖氨酸脱羧酶(细胞)使用稳定性差的问题，极大降低载体的使用成本和1,5‑戊二胺生物法生产成本，简化1,5‑戊二胺溶液和酶的分离步骤，加大1,5‑戊二胺生产的自动化程度，推进了生物法生产1,5‑戊二胺的产业化进程。

Support for holding a complexed enrichment of degrading bacteria and manufacturing method thereof, novel bacteria and method of cleaning polluted environment and device thereof

The present invention provides a stable complex microbial system, which simultaneously decomposes a plurality of organic contaminants even under a polluted environment with these contaminants and permits more effectivedecomposition of persistent organic contaminants such as PCNB and simazine. A support for holding a complexed enrichment of degrading bacteria, which contains a porous material provided as a support on which degrading bacteria A capable of degrading at least one organic contaminant and degrading bacteria B capable of degrading another organic contaminant are enriched, is produced. The degrading bacteria A may be a PCNB-degrading bacteria, particularly degrading bacteria containing degrading bacteria having part or all of the bacteriological characteristics of Nocardioides sp. PD653 and the degrading bacteria B may be degrading bacteria containing degrading bacteria having part or all of the bacteriological characteristics of .beta.-Proteobacteria CDB21.

Immobilization of invertase on polyethylenimine- coated cotton cloth

ABSTRACT OF THE DISCLOSURE A process is provided herein for the immobilization of an enzyme on the surface of cotton cloth. The cotton cloth so provided is also novel. The process comprises treating the cloth with a chemi-cal agent e.g. polyethylenimine to provide an ionic surface thereon; adsorbing the enzyme, e.g. invertase on that surface by ionic inter-action; and immobilizing the adsorbed enzyme on the cloth by cross-linking with a cross-linking agent, e.g. glutaraldehyde. The enzyme immobilized on the cloth by this technique exhibits good activity and stability.

Water insoluble enzyme prepn and improved method for 6-amino-penicilla - nic acid

(A) A water insoluble enzyme prepn. (I) (B) Improved method for 6-aminopenicillanic acid. Deacylation of penicillin. A suitable acylase, obtd. e.g. from E. coli or fungal sources, is reacted with a suitable reactive carboxymethylcellulose, Zeocarb 226 acid chloride, or hydrazine cross-linked ethylene-maleic anhydride resins, tog. with the appropriate amide-forming reagent, e.g. DCCD. The coupled enzyme is esp. useful of for deacylation of benzyl and phenoxymethylpenicillins to 6-aminopenicillanic acid of high purity.

Plant-derived cell culture material

Continuous process for the enzymatic preparation of isomaltulose

The present invention relates to a continuous process for the enzymatic preparation of isomaltulose. A periplasmatic sucrose-mutase is produced by fermentation of microorganisms which form sucrose-mutase. The cell-free crude enzyme extract is prepared by digestion of the cells and by cross-flow microfiltration. In a single-stage, simultaneous purification and immobilization of the sucrose-mutase from the cell-free crude extract conditioned by diafiltration is obtained by selective bonding to an anionizable carrier matrix. Direct conversion of sucrose into isomaltulose is produced by the sucrose-mutase bonded to the anionizable carrier matrix, preferably in cartridge or cartouche form.

Carrier for proteins

1444395 Coated materials L GIVAUDAN & CIE SA 12 July 1973 [12 July 1972] 33288/73 Heading B2E [Also in Divisions Cl and C3] A protei-free carrier material for fixing proteins thereon comprises a water-insoluble polymer or water-insoluble inorganic material containing reactive oxygen functions provided with a layer of diazotised aromatic diamine. The carrier material may 'be produced by reacting the polymer or inorganic material with an aromatic diamine and diazotising the reaction product. Examples of water-insoluble inorganic materials are silicates and silicatescontaining materials such as sand, stone, silica gel or siliceous earth, bentonite, woolastonite or glass, metal oxides sudh as aluminium oxide and hydroxyl apatite. Suitable water-insoluble polymers are polyamides and cellulose e.g. cotton. Water-insoluble protein preparations may be produced by treating the carrier material with a protein solution, e.g. in a buffer solution at pH 5-7.8. Proteins which may be coupled to the carrier materials include antigens, antibodies, inhibitors such as tryptin inhibitors and enzymes. In a typical example silica gel is added to a benzene solution of 4, 4-diamino diphenyl methane and the reaction product diazotised with sodium nitrile and hydrochloric acid at 0‹C. The resulting particles are used for coupling with an enzyme

Microorganism immobilization

ABSTRACT OF THE DISCLOSURE An improved repetitive batch fermentation process is provided herein. It includes the step of freely suspending and stirring small segments of support cloth, bearing a fixed film of live and reproductive microorganisms immobilized thereon, within a fermenter containing suitable nutrient liquor to carry out a fermentation process, whereby the microorganisms produce a fermented liquor. The support cloth bearing the fixed film of live and reproductive microorganisms immobilized thereon is separated from the fermented liquid and residual nutrient liquor in the fermenter. Fermented liquor and residual nutrient liquor are then withdrawn from the fermenter while the support cloth is retained within the fermenter. Fresh nutrient is added to the fermenter containing the support cloth bearing the fixed film of live and reproductive microorganisms immobilized thereon to carry out another the fermentation process. Steps (a), (b), (c) and (d) may be repeated for any desired number of times. This provides an improved batch fermentation process which minimizes the problems of start-up and which can be readily scaled-up and automated.

A kind of process for fixation of Escherichia coli and the method using immobilization Escherichia coli fed-batch fermentation production L lysines

本发明公开了一种大肠杆菌的固定化方法，包括将活化的大肠杆菌接入装有培养基、经过预处理的多孔网状材料和MOPS的容器中进行固定化培养，使大肠杆菌在固定化培养过程中吸附于多孔网状材料上。本发明还公开了一种利用固定化大肠杆菌补料发酵生产L‑赖氨酸的方法，包括发酵过程和补料过程，所述补料过程使用的营养成分为葡萄糖和硫酸铵。MOPS可以增强大肠杆菌在固定化材料表面的粘附效果；利用本发明的固定化大肠杆菌发酵L‑赖氨酸，L‑赖氨酸浓度由游离细胞发酵的90g/L提高至136g/L，重复发酵8批次仍能保持较高的L‑赖氨酸生产效率，平均每个批次最终L‑赖氨酸产量150g/L。

Polymer beads containing an immobilized extractant for sorbing metals from solution

Polymer beads are prepared containing an immobilized extractant for sorbing metal contaminants at concentrations of less than 1 mg/L in dilute aqueous solutions. A preferred polymer is polysulfone and the extractant can be a biomass material or a synthetic chemical compound sorbed into activated carbon. The polymer beads are prepared by dissolving the polymer in an organic solvent to form a solution, adding the extractant to the solution to form a mixture and injecting the mixture through a nozzle into water to form the beads.

Insoluble penicillin acylase

A kind of method of silanization magnetic cellulose microsphere fixed fat enzyme

本发明涉及一种硅烷化磁性纤维素微球固定脂肪酶的方法，目的在于提供一种强度大，易回收的生物基质材料用于固定化脂肪酶的制备方法。所采取的技术方案是:以纤维素为原料，采用反相悬浮技术以及共沉淀法制备出磁性纤维素微球，利用硅烷化改性技术，引入强度较大的Si‑O键，制备出强度较大的硅烷化磁性纤维素微球，最后采用交联—共价键法将游离脂肪酶固定在材料上，得到一种硅烷化磁性纤维素微球固定化脂肪酶催化剂。该方法所使用的原料来源广、无污染，制备的硅烷化磁性微球较其他磁性纤维素微球强度大、重复使用次数高，能广泛应用于能源、医药和化工等领域。

Fiber for clothing and production method therefor

A fiber for clothing, the fiber having a layer of crosslinked enzyme protein on a surface of a single fiber or a monofilament thereof; and a method for producing the fiber for clothing having the steps of immersing a fiber into a solution containing an enzyme protein e.g. hydrolase, to adsorb the enzyme protein onto a surface of a single fiber or a monofilament thereof, and crosslinking the enzyme protein adsorbed on the surface of the single fiber or the monofilament with a crosslinking agent.

The preparation of an immobilized glucose isomerase enzyme composition

Product and process for increasing enzyme adsorption

ABSTRACT OF THE INVENTION This invention provides a process for increasing the adsorption capacity of DEAE-cellulose composites by subjecting the compounds to one or more treatments in an aqueous medium at an elevated temperature.

ACYLASES FIXED TO A CELLULOSE BASED SUPPORT

L'invention concerne un procédé de préparation d'acylases fixées sur un support. Selon l'invention, on fait réagir une acylase soluble avec des groupes réactifs fixés chimiquement de dérivés de cellulose obtenus par fonctionnalisation de la cellulose dans des solutions de cette dernière dans du diméthylsulfoxyde contenant du polyoxyméthylène. Application Produits d'immobilisation d'acylases. The invention relates to a process for the preparation of supported acylases. According to the invention, a soluble acylase is reacted with chemically attached reactive groups of cellulose derivatives obtained by functionalization of cellulose in solutions of the latter in dimethylsulfoxide containing polyoxymethylene. Application Acylase immobilization products.

Nitrifying bacteria agent for industrial wastewater and preparation method thereof

本发明提供了一种用于工业废水的硝化菌剂，该硝化菌剂主要由菌种、营养成分以及固体基质为原料制得，所述菌种包括有效活菌数不低于10亿/g的节杆菌和不动杆菌，所述营养成分包括柠檬酸钠2‑6份、甘油2‑10份、亚硝酸钠2‑6份、抗坏血酸1‑2份、乙二胺四乙酸0.5份、核黄素0.2份。本发明提供的一种用于工业废水的硝化菌剂,以固体基质为骨架，节杆菌、不动杆菌、柠檬酸钠、甘油、亚硝酸钠、抗坏血酸、乙二胺四乙酸、核黄素制得，通过该原料制得的硝化菌剂，在处理高污染工业废水时效率高，使用量少，在水体中形成的菌落结构均衡；同时，本发明以固体基质为骨架，可较长时间保持生物活性，有效延长产品储存周期。

Hydrogel compositions comprising protist cells

The present disclosure relates to hydrogels composition comprising protist cells. In particular, the present disclosure relates to hydrogel compositions which may be used to encapsulate or suspend ciliated protist cells, and methods of preparing the same. The present disclosure further relates to methods of infecting molluscs with a ciliated protist cell, and methods and compositions for stabilising ciliated protist cells.

Multi-Layered Film Containing Nutrient Materials for Cell Culture, Preparing Mehtod and Uses Thereof

본 출원은 세포 배양용 영양물질을 포함하는 다층필름, 이의 제조방법 및 이의 용도에 관한 것이다. 본 출원의 다층필름은 배양육 제조를 위한 세포배양에 유용하게 사용될 수 있다.

Encapsulation method

Biological material such as proteins and microbes is encapsulated by a method which comprises a) admixing the biological material with an aqueous non-ionic polymer solution in which the polymer is present at a concentration of at least 3% w/v; b) forming polymeric beads by adding the mixture of part (a) dropwise into a water-immiscible non-solvent for said polymer, said non-solvent being maintained at a temperature sufficient to freeze the bead but not so low to cause freeze fracture; and c) drying the beads of step (b) to remove substantially all unbound water in said beads. Freeze-drying produces a stable encapsulated product which in the case of microbes retains a high level of viability. The method provides a means of using microbes as agricultural agents since the encapsulated product is acceptable and useful to the farmer.

Patent FR2200281B1

Process for the production of enzyme bodies for the implementation of enzymatic reactions

Process for the preparation of an immobilized glucose isomerase enzyme complex

Enzymatic synthesis of polynucleotides

Patent FR2418237B1

Stable storage of proteins

The present invention provides a method of stably storing a protein, the method comprising applying a protein to be stored to a substrate which has been treated with a polyhydric compound and dried, wherein the amount of the polyhydric compound present in the substrate is sufficient to stabilise the protein, and wherein the substrate does not consist of glass. In one embodiment the protein to be stored is trypsin.

Deacylase enzyme preparations

Carrier for immobilizing microorganisms, and method for converting nitrogen compounds in a liquid using the same

A carrier for immobilizing microorganisms, comprising a porous cellulose derivative or a porous cellulose, which has a good affinity for the microorganisms, can be applied to any liquid treatment tank without limiting use sites, and does not require a plant investment for immobilization of the microorganisms, and a method of converting nitrogen compounds in a liquid to be treated such as potable water or wastewater, by introducing the carrier together with the liquid to be treated into a liquid treatment tank such as a nitrification treatment tank or a denitrification treatment tank, thereby converting the nitrogen compounds contained in the liquid to be treated into harmless nitrogen compounds, are disclosed.