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Небесная энциклопедия

Космические корабли и станции, автоматические КА и методы их проектирования, бортовые комплексы управления, системы и средства жизнеобеспечения, особенности технологии производства ракетно-космических систем

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Мониторинг СМИ

Мониторинг СМИ и социальных сетей. Сканирование интернета, новостных сайтов, специализированных контентных площадок на базе мессенджеров. Гибкие настройки фильтров и первоначальных источников.

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Поддерживает ввод нескольких поисковых фраз (по одной на строку). При поиске обеспечивает поддержку морфологии русского и английского языка
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Применить Всего найдено 1977. Отображено 100.
08-03-2012 дата публикации

Sweetener composition comprising high solubility form of rebaudioside a and method of making

Номер: US20120058236A1
Автор: Allan S. Myerson, Christopher Austin Tyler, Guo-Hua Zheng, John Joseph Hahn, Lawrence E. Fosdick, Troy Allen Rhonemus, Yauching W. Jasinski
Принадлежит: Cargill Inc

Disclosed are sweetener compositions comprising rebaudioside A and methods of making the sweetener compositions. The sweetener compositions comprise a highly soluble crystal form of rebaudioside A that displays, in some embodiments, a solubility at 24° C. in water of about 25 (grams rebaudioside A/per 100 grams water) or greater. The high solubility of the sweetener composition of the invention allows it to be used in applications such as syrups and concentrates.

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Номер записи: 1
04-07-2013 дата публикации

PRODUCTION OF STEVIOL GLYCOSIDES IN MICROORGANISMS

Номер: US20130171328A1
Автор: Blom Charlotte, Dalgaard Mikkelsen Michael, Halkjær Hansen Esben, Hansen Jorgen, HICKS Paula M., Houghton-Larsen Jens, Kishore Ganesh M., Motion Michael, Tavares Sabina
Принадлежит:

Recombinant microorganisms, plants, and plant cells are disclosed that have been engineered to express novel recombinant genes encoding steviol biosynthetic enzymes and UDP-glycosyltransferases (UGTs). Such microorganisms, plants, or plant cells can produce steviol or steviol glycosides, e.g., rubusoside or Rebaudioside A, which can be used as natural sweeteners in food products and dietary supplements. 1. A recombinant host comprising:a) a recombinant gene encoding a UGT91D2 polypeptide:b) a recombinant gene encoding a UGT85C polypeptide having at least 90% identity to the amino acid sequence set forth in SEQ ID NO:3 such as a UGT85C polypeptide comprising one or more amino acid substitutions at residues 9, 10, 13, 15, 21, 27, 60, 65, 71, 87, 91, 220, 243, 270, 289, 298, 334, 336, 350, 368, 389, 394, 397, 418, 420, 440, 441, 444, and 471 of SEQ ID NO:3; and/orc) a recombinant gene encoding a UGT76G1 polypeptide having at least 90% identity to the amino acid sequence set forth in SEQ ID NO:7, and having one or more amino acid substitutions at residues 29, 74, 87, 91, 116, 123, 125, 126, 130, 145, 192, 193, 194, 196, 198, 199, 200, 203, 204, 205, 206, 207, 208, 266, 273, 274, 284, 285, 291, 330, 331, and 346.2. The recombinant host of claim 1 , wherein said UGT91D2 polypeptide is UGT91D2e or UGT91D2m.3. The recombinant host of claim 1 , wherein said UGT91D2 polypeptide has at least 90% claim 1 , 95% claim 1 , or 99% identity to the amino acid sequence set forth in SEQ ID NO:5.412.-. (canceled)13. The host of claim 1 , wherein said UGT91D2 polypeptide comprises i) at least one amino acid substitution at residues 1-19 claim 1 , 27-38 claim 1 , 44-87 claim 1 , 96-120 claim 1 , 125-141 claim 1 , 159-184 claim 1 , 199-202 claim 1 , 215-380 claim 1 , or 387-473 of SEQ ID NO:5; ii) an amino acid substitution at one or more residues selected from the group consisting of residues 30 claim 1 , 93 claim 1 , 99 claim 1 , 122 claim 1 , 140 claim 1 , 142 claim 1 , 144 claim 1 , ...

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Номер записи: 2
11-07-2013 дата публикации

ANTIBACTERIAL AMINOGLYCOSIDE ANALOGS

Номер: US20130178438A1
Автор: Aggen James Bradley, Dozzo Paola, Gliedt Micah James, Goldblum Adam Aaron, Hildebrandt Darin James, Kane Timothy Robert, Linsell Martin Sheringham
Принадлежит: Achaogen, Inc.

Compounds having antibacterial activity are disclosed. The compounds have the following structure (I): 127-. (canceled)29. A pharmaceutical composition according to claim 28 , wherein Ris C-Calkyl substituted with one hydroxyl.30. A pharmaceutical composition according to claim 29 , wherein Ris —(CH)OH claim 29 , wherein m is an integer from 2 to 6.31. A pharmaceutical composition according to claim 30 , wherein Ris —(CH)OH or —(CH)OH.32. A pharmaceutical composition according to claim 31 , wherein Ris —(CH)OH.33. A pharmaceutical composition according to claim 29 , wherein Ris —(CH)CH(CH)OH claim 29 , wherein p is an integer from 1 to 4.34. A pharmaceutical composition according to claim 33 , wherein Ris —CHCH(CH)OH or —(CH)CH(CH)OH.35. A pharmaceutical composition according to claim 28 , wherein Ris C-Calkyl substituted with two hydroxyl groups.36. A pharmaceutical composition according to claim 35 , wherein Ris —CHCH(OH)(CH)OH wherein q is an integer from 1 to 4.37. A pharmaceutical composition according to claim 36 , wherein Ris —CHCH(OH)CHOH or —CHCH(OH)(CH)OH.38. A pharmaceutical composition according to claim 35 , wherein Ris —(CH)CH(CHOH)wherein r is 0 or an integer from 1 to 3.39. A pharmaceutical composition according to claim 38 , wherein Ris —CHCH(CHOH)or —CH(CHOH).40. A pharmaceutical composition according to claim 39 , wherein Ris hydrogen. This application is a continuation of U.S. patent application Ser. No. 13/295,219, filed Nov. 14, 2011, which claims the benefit under 35 U.S.C. §119(e) of U.S. Provisional Patent Application No. 61/414,762, filed Nov. 17, 2010, and U.S. Provisional Patent Application No. 61/505,371, filed Jul. 7, 2011. The foregoing applications are incorporated herein by reference in their entireties.This invention was made with government support under Contract No. HHSN272200800043C, awarded by the National Institutes of Health. The government has certain rights in this invention.1. FieldThe present invention is directed to novel ...

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Номер записи: 3
12-09-2013 дата публикации

Sweetening compositions including rebaudiosides and methods of preparation

Номер: US20130236629A1
Автор: Gino E. Olcese, Sarah M. Erdman, Stacey K. Walton
Принадлежит: Dr Pepper Seven Up Inc

A method of preparing a sweetening composition may include providing a stevia extract and heating the extract under acidic conditions. Treatment may subject the stevia extract to conditions sufficient to alter the glycoside composition of the extract, suppress bitter aftertastes, or provide a sweetening composition with a taste profile similar to that of sucrose sugar.

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Номер записи: 4
28-11-2013 дата публикации

Glucosylated steviol glycosides as a flavor modifier

Номер: US20130316043A1
Автор: Siddhartha Purkayastha
Принадлежит: Individual

A taste and flavor profile modifying composition is described. The composition includes glucosylated steviol glycosides which can modify the intensity of a taste and/or a flavor in a food or beverage product.

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Номер записи: 5
23-01-2014 дата публикации

Glucosylated steviol glycosides composition as a flavor modifier

Номер: US20140023750A1
Автор: Siddhartha Purkayastha
Принадлежит: PureCircle USA Inc

A taste and flavor profile enhancing composition includes glucosylated steviol glycosides which can enhance the intensity of a taste and/or a flavor in a food or beverage product. A method of increasing the taste and flavor intensity of a food or beverage product, includes the step of adding a taste and flavor enhancing composition to the food or beverage product, wherein the taste and flavor enhancing composition includes glucosylated steviol glycosides. A method of improving the organoleptic properties of a food or beverage product including a high fructose syrup, including the step of adding the taste and flavor enhancing composition to the food or beverage product. Adding the taste and flavor enhancing composition may cause the high fructose syrup, such as high fructose corn syrup, to taste more like sugar.

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Номер записи: 6
23-01-2014 дата публикации

Microbial method for the biotransformation of colchicinoid compounds

Номер: US20140024080A1
Автор: Ayyappan Hariharan Dakarakkaran, Bhimaraju Poliseti, Krishnamurthy Prabakaran, Nallasamy Mohanasundaram Kannamapuram, Sankarasubramanian Kanagavelrajan
Принадлежит: Elysian Life Sciences Pvt Ltd

The invention relates to a method for the Bio-transformation of Thiocolchicine a colchicinoid compound into its glycosylated form using a selective microorganism Providencia vermicola . Use of bacterial strain, Providencia vermicola for microbial bio-transformation of thiocolchicine (TCN) to their corresponding 3-0-glycosyl derivative as well as specific strains of that species Providencia vermicola for microbial transformation of other colchicinoid compounds such as colchicine, thiocolchicine, 3-demethylcolchicine, 3-demethylthiocolchicine and N-deacetylthiocolchicine to its corresponding glycosylated form and subsequent isolation of the transformed compounds from the bacterial culture medium is disclosed. Providencia vermicola (MTCC 5578) a gram negative bacteria has been identified having trans-glycosylation ability and convert thiocolchicine (TCN) to Thiocolchicocide (TCS).

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Номер записи: 7
13-02-2014 дата публикации

Sweetener crystals and method of making

Номер: US20140044852A1
Автор: Joseph R. PANARISI, Jr., Shyhyuan Liao
Принадлежит: Mcneil Nutritionals Llc

A sweetening composition comprising a crystalline matrix wherein the crystalline matrix comprises a high intensity sweetener and a carrier/substrate, and wherein the composition has a bulk density of about 0.4 g/cc to about 1.0 g/cc.

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Номер записи: 8
20-03-2014 дата публикации

Enhanced natural sweetener

Номер: US20140079870A1
Автор: Juan L. Navia, Steven J. Catani
Принадлежит: Mcneil Nutritionals Llc

A sweetening composition comprising stevia extract that contains steviol glycosides and other non-volatile components found in stevia leaves, wherein the steviol glycosides and other non-volatile components combined are less than about 80 wt % of the stevia extract.

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Номер записи: 9
04-01-2018 дата публикации

CONSUMABLES

Номер: US20180000141A1
Автор: HANSEN Chad Allen, SIMONS Christopher Todd, SLACK Jay Patrick
Принадлежит:

Disclosed are sweetened consumables and methods of forming said sweetened consumables that comprise certain sweeteners and at least one sweetness enhancer in a concentration near its sweetness detection threshold. The sweeteners include sucrose, fructose, glucose, high fructose corn syrup, corn syrup, xylose, arabinose, rhamnose, erythritol, xylitol, mannitol, sorbitol, inositol, acesulfame potassium, aspartame, neotame, sucralose, saccharine, or combinations thereof. The sweetness enhancer is selected from naringin dihydrochalcone, mogroside V, swingle extract, rubusoside, extract, rebaudioside, and stevioside. 1. A sweetened consumable comprising wherein said sweetener comprises sucrose, fructose, glucose, high fructose corn syrup, corn syrup, xylose, arabinose, rhamnose, erythritol, xylitol, mannitol, sorbitol, inositol, acesulfame potassium, aspartame, neotame, sucralose, saccharine, or combinations thereof,', 'wherein said at least one sweetener or sweetener combination is present in a concentration above the sweetness detection threshold in a concentration at least isosweet from about 2% to about 15% sucrose, and, 'a) at least 0.0001% of at least one sweetener,'}{'i': 'rubus', 'claim-text': {'i': 'rubus', 'wherein said sweetness enhancer is present in a concentration near its sweetness detection threshold, and wherein for naringin dihydrochalcone this concentration is from 2 to 60 ppm, for rubusoside from 1.4 ppm to 56 ppm, for extract from 2 ppm to 80 ppm, for mogroside V from 0.4 ppm to 12.5 ppm, for swingle extract from 2 to 60 ppm, for stevioside from 2 to 60 ppm, and for rebaudioside A from 1 to 30 ppm.'}, 'b) at least one sweetness enhancer selected from the group consisting of naringin dihydrochalcone, mogroside V, swingle extract, rubusoside, extract, stevioside, rebaudioside A, and combinations thereof'}2. The sweetened consumable of comprising naringin dihydrochalcone as a sweetness enhancer.3rubus. The sweetened consumable of comprising rubusoside ...

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Номер записи: 10
04-01-2018 дата публикации

REBAUDIOSIDE B AND DERIVATIVES

Номер: US20180000142A1
Автор: Deng Mingming, Ding Jien, Shi Jingang, Wang Hansheng, Wu Yanyan
Принадлежит:

The invention describes compositions that include a stevia sweetener and a salt of a steviol glycoside, wherein the concentration of the components provide an improved taste profile where bitterness, after taste and/or lingering of the stevia sweetener is decreased or eliminated. 166-. (canceled)67. A stevia composition comprising a mixture consisting essentially of stevia extract , wherein the mixture contains 1% to 30% of purified rebaudioside B by weight , wherein the purity of the rebaudioside B is at least 95% pure , wherein the stevia extract has above 90% purity of total steviol glycoside and the total steviol glycosides contain rebaudioside A and rebaudioside D , wherein the ratio of rebaudioside D to rebaudioside A is from 30:70 to 5:95 by wright , and wherein the ratio of rebaudioside A to rebaudioside B is from 75:25 to 95:5 by weight.68. The stevia composition of claim 67 , wherein the ratio of rebaudioside A to rebaudioside B is from 80:20 to 95:5 by weight.69. The stevia composition of claim 67 , wherein the ratio of rebaudioside A to rebaudioside B is from 85:15 to 95:5 by weight.70. The stevia composition of claim 67 , wherein the ratio of rebaudioside A to rebaudioside B is 90:10 by weight.71. The stevia composition of claim 67 , wherein the rebaudioside B comprises a salt of rebaudioside B.72. The stevia composition of claim 67 , wherein the rebaudioside B comprises an amino acid salt of rebaudioside B.73. The stevia composition of claim 67 , wherein rebaudioside C is absent from the stevia composition.74. The stevia composition of claim 73 , wherein one or more components selected from the group consisting of rebaudioside E claim 73 , F claim 73 , stevioside claim 73 , steviolmonoside claim 73 , steviolbioside claim 73 , rubusoside claim 73 , and dulcoside are absent from the stevia composition.75. A stevia composition comprising rebaudioside A claim 73 , rebaudioside B and rebaudioside D claim 73 , wherein the ratio of rebaudioside A to ...

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Номер записи: 11
06-01-2022 дата публикации

HIGH-PURITY RUBUSOSIDE AND PROCESS FOR PRODUCING OF THE SAME

Номер: US20220002774A1
Автор: MARKOSYAN Avetik
Принадлежит:

The invention provides a process of producing Rubusoside from steviol glycosides of plant. The process is useful for producing high purity Rubusoside with purity greater than 95% (dry basis). High purity rubusoside is useful as in combination with other caloric and non-caloric sweeteners as well as non-caloric sweetener in various food and beverage compositions. The high purity rubusoside is useful as non-caloric sweetener in edible and chewable compositions such as any beverages, confectionaries, bakeries, cookies, chewing gums, and alike. 1Stevia rebaudiana. A process for preparing Rubusoside from steviol glycosides of comprising the steps of:{'i': 'Stevia rebaudiana;', 'a. providing initial steviol glycosides of'}b. dissolving the initial steviol glycosides in the water;c. providing an enzyme with glycosyl hydrolase activity to obtain reaction mixture;d. incubating the reaction mixture to facilitate complete or partial transformation of Stevioside to Rubusoside;e. terminating the reaction by thermal inactivation of enzyme;f. recovering and purifying high purity Rubusoside from reaction mixture.2Stevia. A process of wherein step (a) the steviol glycoside is extract claim 1 , mixture of steviol glycosides or pure Stevioside.3. A process of wherein step (b) the concentration of dissolved steviol glycosides is 1-50% (wt/vol) claim 1 , preferably 5-30% (wt/vol) claim 1 , more preferably 8-10% (wt/vol).4. A process of wherein step (c) the enzyme is selected from the group of rhamnosidase claim 1 , β-glucosidase claim 1 , hesperidinase claim 1 , naringinase claim 1 , pectinase claim 1 , cellulase claim 1 , and other enzymes with glycosyl hydrolase activity claim 1 , in free or immobilized forms.5. A process of wherein step (d) the molar yield of Rubusoside from Stevioside is 1-100% claim 1 , preferably 80-100% and more preferably 95-100%.6. A process of wherein step (e) the reaction termination includes heat treatment claim 1 , activated carbon treatment.7. A process of ...

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Номер записи: 12
07-01-2021 дата публикации

Increased production of ginsenosides through yeast cell organelle improvement

Номер: US20210002334A1
Автор: JANG In Seung, JEGAL Jong Geon, JUNG Suk Chea, Kim Seohyun, Kim Sun-Chang, Lee Ju Young
Принадлежит:

Provided are a recombinant yeast having improved ability to produce ginsenoside, which is prepared by overexpressing INO2 and INO4 or deleting OPT1 in a yeast having ability to produce ginsenoside, a method of preparing the yeast, and a method of producing ginsenoside by using the yeast. 115-. (canceled)16. A method of preparing a recombinant yeast having improved ability to produce ginsenoside , the method comprising the step of changing an expression level of a transcription factor of a phospholipid biosynthetic gene in a ginsenoside or ginsenoside precursor producing yeast strain.17. The method of claim 16 , wherein an expression level of the phospholipid biosynthetic gene claim 16 , INO2(INOsitol requiring 2) or INO4(INOsitol requiring 4) claim 16 , or expression levels of both of them is/are increased claim 16 , as compared with their intrinsic expression levels claim 16 , or an expression level of OPI1(OverProducer of Inositol 1) is decreased claim 16 , as compared with its intrinsic expression level.18S. cerevisiae. The method of claim 16 , wherein the ginsenoside-producing yeast strain has increased expression levels of one or more genes selected from the group consisting of PgDDS (Panax ginseng claim 16 , dammarenediol-II synthase) claim 16 , PgPPDS (Panax ginseng cytochrome P450 CYP716A47) claim 16 , PgCPR (Panax ginseng NADPH-cytochrome P450 reductase) claim 16 , tHMG1 (HMG-CoA reductase) claim 16 , and PgSE (Panax ginseng claim 16 , squalene epoxidase) claim 16 , as compared with their intrinsic expression levels.19. The method of claim 16 , wherein the INO2(INOsitol requiring 2) gene is composed of a nucleotide sequence of SEQ ID NO: 1 claim 16 , the INO4(INOsitol requiring 4) gene is composed of a nucleotide sequence of SEQ ID NO: 2 claim 16 , and the OPI1(OverProducer of Inositol 1) gene is composed of a nucleotide sequence of SEQ ID NO: 320Saccharomyces cerevisiaeS. cerevisiaeSaccharomyces bayanusS. bayanusSaccharomyces boulardiiS. ...

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Номер записи: 13
02-01-2020 дата публикации

BIOSYNTHETIC PRODUCTION OF STEVIOL GLYCOSIDE REBAUDIOSIDE I VIA VARIANT ENZYMES

Номер: US20200002742A1
Автор: Batten Michael, Hunt Phillip, Mao Guohong, Yu Xiaodan
Принадлежит: Conagen Inc.

The present invention relates, at least in part, to the production of steviol glycoside rebaudioside I through the use of variant UGT enzymes having activity to transfer a glucosyl group from UDP-glucose to rebaudioside A to produce rebaudioside I. 1. A method for synthesizing rebaudioside I , the method comprising preparing a reaction mixture comprising:(a) a steviol glycoside composition comprising rebaudioside A;(b) a substrate selected from the group consisting of sucrose, uridine diphosphate (UDP), uridine diphosphate-glucose (UDP-glucose), and combinations thereof; and(c) a UDP-glycosyltransferase enzyme comprising the amino acid sequence of SEQ ID NO: 1; andincubating the reaction mixture for a sufficient time to produce rebaudioside I.2stevia. The method of claim 1 , wherein the steviol glycoside composition is extract.3. The method of claim 1 , further comprising adding a sucrose synthase to the reaction mixture.4Arabidopsis thaliana. The method of claim 3 , wherein the sucrose synthase is an sucrose synthase 1 (AtSUS1) comprising the amino acid sequence of SEQ ID NO: 11.5. The method of claim 1 , wherein the reaction mixture is in vitro.6. The method of . wherein the reaction mixture is a cell-based reaction mixture.7. The method of claim 6 , wherein the UDP-glycosyltransferase enzyme is expressed in a host cell.8. The method of claim 7 , wherein the host cell is selected from the group consisting of a yeast claim 7 , a non-steviol glycoside producing plant claim 7 , an alga claim 7 , a fungus claim 7 , and a bacterium.9. The method of claim 7 , wherein the host cell is a bacterial cell.10E. coli. The method of claim 9 , wherein the bacterial cell is an cell.11. The method of claim 7 , wherein the host cell is a yeast cell.12. The method of claim 1 , wherein the subject is UDP-glucose.13. The method of claim 1 , wherein the rebaudioside A has a concentration of 15 to 50 g/L in the reaction mixture.14. The method to claim 1 , wherein the reaction mixture ...

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Номер записи: 14
07-01-2021 дата публикации

BIOSYNTHETIC PRODUCTION OF STEVIOL GLYCOSIDES AND PROCESSES THEREFORE

Номер: US20210002685A1
Автор: Batten Michael, Byun David, Luo Yang, Mao Guohong, Vick Jacob Edward, Wu Yilin, Yu Xiaodan, Zhang Beihua
Принадлежит: Conagen Inc.

The present invention relates to the production of steviol glycoside rebaudiosides D4, WB1 and WB2 and the production of rebaudioside M from Reb D4. 154-. (canceled)56. The method of claim 55 , wherein the UDP-glycosyltransferase is UGT76G1 having the amino acid sequence of SEQ ID NO: 1 or a functional homolog thereof.57. The method of claim 55 , wherein the UDP-glycosyltransferase comprises an amino acid sequence having at least 80% identity to the amino acid sequence of SEQ ID NO: 3.58. The method of claim 55 , wherein the UDP-glycosyltransferase is a mutant of an enzyme having the amino acid sequence of SEQ ID NO: 3 claim 55 , wherein the mutant comprises at least one mutation at an amino acid residue position corresponding to a position selected from 3 claim 55 , 6 claim 55 , 90 claim 55 , 91 claim 55 , 93 claim 55 , 181 claim 55 , 183 claim 55 , 184 claim 55 , 185 claim 55 , 350 claim 55 , 389 claim 55 , 410 claim 55 , 418 claim 55 , 450 claim 55 , 451 claim 55 , 452 claim 55 , and 454 of SEQ ID NO: 3.59. The method of claim 58 , wherein the UDP-glycosyltransferase is an enzyme having the amino acid sequence of SEQ ID NO: 3 and further having at least one substitution selected from the group consisting of (i) W3L claim 58 , (ii) L6A or L6G claim 58 , (iii) T90A or T90G claim 58 , (iv) S91G or S91L claim 58 , (v) V93A or V93G claim 58 , (vi) S181G claim 58 , (vii) F183V claim 58 , F183A claim 58 , or F183G claim 58 , (viii) G184A claim 58 , (ix) L185A claim 58 , (x) G350A claim 58 , (xi) L389V claim 58 , (xii) S410G claim 58 , (xiii) H418V claim 58 , (xiv) T450A or T450G claim 58 , (xv) K451A claim 58 , (xvi) D452A claim 58 , and (xvii) K454L or K454V.61. The method of claim 55 , wherein the reaction mixture further comprises sucrose claim 55 , uridine diphosphate claim 55 , and a sucrose synthase.62. The method of claim 55 , further comprising rebaudioside M thus produced from the reaction mixture for use as a sweetener. This application is a continuation of ...

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Номер записи: 15
20-01-2022 дата публикации

Efficient Production of Steviol Glycosides in Recombinant Hosts

Номер: US20220017932A1
Автор: Anderson Iben Nordmark, Dalgaard Mikkelsen Michael, Douchin Veronique, Hansen Jorgen, Simon Ernesto
Принадлежит:

Recombinant microorganisms are disclosed that produce steviol glycosides and have altered expression of one or more endogenous transporter or transcription factor genes, or that overexpress one or more heterologous transporters, leading to increased excretion of steviol glycosides of interest. 1. A recombinant microorganism capable of producing a steviol glycoside in a cell culture ,wherein the microorganism has a modified expression of at least one endogenous transporter gene and at least one endogenous transcription factor gene that regulates expression of the at least one endogenous transporter gene,wherein the modified expression comprises reduced expression or activity of the at least one endogenous transporter gene, having at least 80% sequence identity to the amino acid sequence set forth in SEQ ID NO:113;wherein the reduced expression is produced in the microorganism by disrupting or deleting the gene locus for the at least one endogenous transporter gene;wherein the microorganism further expresses at least one heterologous transporter gene;wherein the steviol glycoside is Rebaudioside A, Rebaudioside B, Rebaudioside D, Rebaudioside E, Rebaudioside M, or an isomer thereof.2. The recombinant microorganism of claim 1 , wherein the endogenous and/or the heterologous transporter gene encodes an ATP-Binding Cassette (ABC) transporter.3Stevia. The recombinant microorganism of claim 1 , wherein the heterologous transporter gene is a transporter gene.4. The recombinant microorganism of claim 1 , further comprising a gene encoding a polypeptide capable of beta 1 claim 1 ,2 glycosylation of the C2′ of the 13-O-glucose claim 1 , 19-O-glucose claim 1 , or both 13-O-glucose and 19-O-glucose of a steviol glycoside; wherein the gene has a copy number of 2 or more; and wherein the polypeptide comprises a polypeptide having at least 90% sequence identity to one of the amino acid sequences set forth in any one of SEQ ID NOs:51 claim 1 , 54 claim 1 , 55 claim 1 , 86 claim 1 , ...

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Номер записи: 16
14-01-2016 дата публикации

METHOD FOR PREPARING REBAUDIOSIDE A FROM STEVIOSIDE

Номер: US20160010133A1
Автор: Hong Young Ho, KIM Jung Eun, Kim Seong Bo, Park Seung Won, Park Sung Hee, Yoon Ran Young
Принадлежит:

The present invention relates to an in-situ method for preparing rebaudioside A from sucrose and stevioside by sucrose synthase and glycosyltransferase. 1. A method for preparing rebaudioside A , comprising:(1) reacting sucrose and nucleotide diphosphate in the presence of sucrose synthase to prepare nucleotide diphosphate to which glucose is bonded; and(2) reacting the nucleotide diphosphate to which glucose is bonded with stevioside in the presence of glycosyltransferase to prepare rebaudioside A.2Arabidopsis thaliana. The method for preparing rebaudioside A according to claim 1 , wherein sucrose synthase is derived from rice claim 1 , corn claim 1 , wheat claim 1 , bamboo claim 1 , claim 1 , grass claim 1 , barley claim 1 , sorghum or potato.3Escherichia coli, BacillusCorynebacteriumAgrobacterium. The method for preparing rebaudioside A according to claim 1 , wherein sucrose synthase is produced from recombinant claim 1 , yeast claim 1 , or transformed with a vector containing a sucrose synthase gene.4. The method for preparing rebaudioside A according to claim 3 , wherein sucrose synthase has a base sequence shown in SEQ ID NO: 3.5Oryza sativa, Stevia rebaudiana Bertoni, Bambusa oldhamii, Brachypodium distachyon, Hordeum vulgare, Sorghum bicolor, Zea maysArabidopsis thaliana.. The method for preparing rebaudioside A according to claim 1 , wherein glycosyltransferase is derived from claim 1 , or6Escherichia coli, BacillusCorynebacteriumAgrobacterium. The method for preparing rebaudioside A according to claim 1 , wherein glycosyltransferase is derived from recombinant claim 1 , yeast claim 1 , or claim 1 , transformed with a vector containing a glycosyltransferase gene.7. The method for preparing rebaudioside A according to claim 1 , wherein glycosyltransferase has a base sequence shown in SEQ ID NO: 4.8. The method for preparing rebaudioside A according to claim 1 , wherein steps (1) and (2) are performed consecutively in-situ.9. The method for preparing ...

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Номер записи: 17
11-01-2018 дата публикации

NON-CALORIC SWEETENER

Номер: US20180009835A1
Автор: CHATURVEDULA VENKATA SAI PRAKASH, Mao Guohong, Yu Xiaodan
Принадлежит: Conagen Inc.

Disclosed is a steviol glycoside referred to as rebaudioside D2. Rebaudioside D2 has five β-D-glucosyl units connected to the aglycone steviol. Also disclosed are methods for producing rebaudioside D2, a UDP-glycosyltransferase fusion enzyme, and methods for producing rebaudioside D and rebaudioside E. 144-. (canceled)45. A method for synthesizing rebaudioside E , the method comprising:preparing a reaction mixture comprising stevioside; a substrate selected from the group consisting of sucrose, uridine diphosphate (UDP) and uridine diphosphate-glucose (UDP-glucose); and a UDP-glycosyltransferase selected from the group consisting of a uridine diphospho glycosyltransferase and a UDP-glycosyltransferase fusion enzyme comprising a uridine diphospho glycosyltransferase domain coupled to a sucrose synthase domain, wherein a glucose is covalently coupled to the stevioside to produce rebaudioside E.46Oryza sativa. The method of claim 45 , wherein the UDP-glycosyltransferase is a uridine diphospho glycosyltransferase EUGT11.47. The method of further comprising adding a sucrose synthase to the reaction mixture.48ArabidopsisCoffeaStevia. The method of claim 47 , wherein the sucrose synthase is selected from the group consisting of an sucrose synthase 1; a sucrose synthase 1 and a sucrose synthase 1.49Arabidopsis thaliana. The method of claim 47 , wherein the sucrose synthase is an sucrose synthase 1.50. The method of claim 45 , wherein the UDP-glycosyltransferase is a UDP-glycosyltransferase fusion enzyme comprising a uridine diphospho glycosyltransferase domain coupled to a sucrose synthase domain.51. The method of claim 50 , wherein the UDP-glycosyltransferase fusion enzyme comprises an amino acid sequence having about 90% sequence identity to SEQ ID NO:5.52Oryza sativa. The method of claim 45 , wherein the uridine diphospho glycosyltransferase domain is an uridine diphospho glycosyltransferase EUGT11.53. The method of claim 45 , wherein the uridine diphospho ...

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Номер записи: 18
11-01-2018 дата публикации

PRODUCTS FROM STEVIA REBAUDIANA

Номер: US20180009910A1
Автор: MARKOSYAN Avetik
Принадлежит: PureCircle Sdn Bhd

Various organic molecules, ingredients and compositions are prepared from plant. The compositions can be used as bulking agents, and sweeteners in foods, beverages, cosmetics and pharmaceuticals. 13-. (canceled)4. A process of producing a stevia ingredient , comprising the steps of:(a) providing a water-insoluble stevia biomass;(b) incubating the water-insoluble stevia biomass in an aqueous alkaline solution comprising at least 10% NaOH to produce a cellulose containing material;(c) processing the cellulose containing material in a pressurized vessel at a temperature of 110-180° C. for 0.1 to 10 hours to provide a stevia-derived feedstock; and(d) contacting the stevia-derived feedstock with a recombinant microorganism.5Stevia rebaudiana. The process of claim 4 , wherein the stevia ingredient is selected from the group consisting of: stevioside claim 4 , rebaudioside A claim 4 , rebaudioside B claim 4 , rebaudioside C claim 4 , rebaudioside D claim 4 , rebaudioside E claim 4 , rebaudioside F claim 4 , rebaudioside G claim 4 , rebaudioside H claim 4 , rebaudioside I claim 4 , rebaudioside J claim 4 , rebaudioside K claim 4 , rebaudioside L claim 4 , rebaudioside M claim 4 , rebaudioside N claim 4 , rebaudioside O claim 4 , rebaudioside P claim 4 , rebaudioside Q claim 4 , rebaudioside R claim 4 , rebaudioside Z claim 4 , dulcoside A claim 4 , steviolbioside claim 4 , rubusoside claim 4 , alpha-glucosylated steviol glycosides claim 4 , steviol glycosides found in plant claim 4 , and combinations thereof.6. The process of claim 4 , further comprising the step of combining the stevia ingredient with a food claim 4 , beverage claim 4 , drug claim 4 , pharmaceutical claim 4 , cosmetic or consumable product.7. The process of claim 4 , further comprising the step of combining the stevia ingredient with a stevia-derived cellulose.8. The process of claim 7 , wherein the stevia-derived cellulose is obtained from a second water-insoluble stevia biomass.9. The process of claim 8 ...

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Номер записи: 19
08-01-2015 дата публикации

High-Purity Rubusoside And Process For Producing Of The Same

Номер: US20150011660A1
Автор: MARKOSYAN Avetik
Принадлежит:

The invention provides a process of producing Rubusoside from steviol glycosides of Stevia rebaudiana plant. The process is useful for producing high purity Rubusoside with purity greater than 95% (dry basis). High purity rubusoside is useful as in combination with other caloric and non-caloric sweeteners as well as non-caloric sweetener in various food and beverage compositions. The high purity rubusoside is useful as non-caloric sweetener in edible and chewable compositions such as any beverages, confectionaries, bakeries, cookies, chewing gums, and alike. 1Stevia rebaudiana. A process for preparing Rubusoside from steviol glycosides of comprising the steps of:{'i': 'Stevia rebaudiana;', 'a. providing initial steviol glycosides of'}b. dissolving the initial steviol glycosides in the water;c. providing an enzyme with glycosyl hydrolase activity to obtain reaction mixture;d. incubating the reaction mixture to facilitate complete or partial transformation of Stevioside to Rubusoside;e. terminating the reaction by thermal inactivation of enzyme;f. recovering and purifying high purity Rubusoside from reaction mixture.2. A process of wherein step (a) the steviol glycoside is Stevia extract claim 1 , mixture of steviol glycosides or pure Stevioside.3. A process of wherein step (b) the concentration of dissolved steviol glycosides is 1-50% (wt/vol) claim 1 , preferably 5-30% (wt/vol) claim 1 , more preferably 8-10% (wt/vol).4. A process of wherein step (c) the enzyme is selected from the group of rhamnosidase claim 1 , β-glucosidase claim 1 , hesperidinase claim 1 , naringinase claim 1 , pectinase claim 1 , cellulase claim 1 , and other enzymes with glycosyl hydrolase activity claim 1 , in free or immobilized forms.5. A process of wherein step (d) the molar yield of Rubusoside from Stevioside is 1-100% claim 1 , preferably 80-100% and more preferably 95-100%.6. A process of wherein step (e) the reaction termination includes heat treatment claim 1 , activated carbon ...

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Номер записи: 20
14-01-2021 дата публикации

URIDINE DIPHOSPHATE-DEPENDENT GLYCOSYLTRANSFERASE ENZYME

Номер: US20210009968A1
Автор: DONALD Jason Eric, KUMARAN Ajikumar Parayil, LOVE Aaron, SANTOS Christine Nicole S., TOOMEY Christopher
Принадлежит:

In various aspects, the present invention provides uridine diphosphate-dependent glycosyltransferase (UGT) enzymes capable of catalyzing the transfer of a monosaccharide moiety from a NDP-sugar to the 3′ carbon of a sugar moiety of a substrate, such as a terpenoid glycan, thereby functioning as a “1-3 UGT.” In other aspects, the invention provides polynucleotides encoding the 1-3 UGT, and host cells comprising the same. In still other aspects, the invention provides methods for preparing glycosylated substrates, including steviol glycosides, using the enzyme and host cells of this disclosure. 1. A uridine diphosphate-dependent glycosyltransferase (UGT) enzyme comprising an amino acid sequence that is at least about 75% identical to the amino acid sequence of SEQ ID NO: 5 or SEQ ID NO: 6.2. The enzyme of claim 1 , wherein the amino acid sequence is at least about 80% identical to SEQ ID NO: 5 or SEQ ID NO: 6.3. The enzyme of claim 1 , wherein the amino acid sequence is at least about 85% identical to SEQ ID NO: 5 or SEQ ID NO: 6.4. The enzyme of claim 1 , wherein the amino acid sequence is at least about 90% identical to SEQ ID NO: 5 or SEQ ID NO: 6.5. The enzyme of claim 1 , wherein the amino acid sequence is at least about 95% identical to SEQ ID NO: 5 or SEQ ID NO: 6.6. The enzyme of claim 1 , wherein the amino acid sequence is at least about 98% identical to SEQ ID NO: 5 or SEQ ID NO: 6.7. The enzyme of claim 1 , wherein the amino acid sequence is the amino acid of SEQ ID NO: 5 or SEQ ID NO: 6.8. The enzyme of claim 1 , wherein the amino acid sequence has from 1 to 20 amino acid modifications independently selected from substitutions claim 1 , deletions claim 1 , and insertions claim 1 , with respect to the amino acid sequence SEQ ID NO: 5 or SEQ ID NO: 6.9. The enzyme of claim 8 , wherein the amino acid sequence has from 1 to 10 amino acid modifications independently selected from substitutions claim 8 , deletions claim 8 , and insertions claim 8 , with respect ...

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Номер записи: 21
17-01-2019 дата публикации

HIGHLY SOLUBLE STEVIOL GLYCOSIDES

Номер: US20190014805A1
Автор: HASIM Mohamad Afzaal Bin, MARKOSYAN Avetik, RAMANDACH Saravanan, TAN KIAN PIN
Принадлежит: PURECIRCLE USA INC.

A method for making a highly soluble steviol glycoside composition is described. The resulting composition readily provides solutions with at least 0.5% concentration. 1. A method for producing a highly soluble steviol glycoside composition comprising the steps of:A) providing a powder composition comprising at least one steviol glycoside;B) providing water;C) mixing the water and powder composition comprising at least one steviol glycoside to make a mixture;D) increasing the temperature of the mixture to make a solution;E) decreasing the temperature of the solution to obtain solidified solution; andF) freeze drying the solidified solution, to provide highly soluble steviol glycoside composition having at least 0.5% solubility in water.2. The method of wherein steviol glycoside is selected from a group consisting of Rebaudioside A claim 1 , Rebaudioside B claim 1 , Rebaudioside C claim 1 , Rebaudioside D claim 1 , Rebaudioside E claim 1 , Rebaudioside F claim 1 , Rebaudioside G claim 1 , Rebaudioside H claim 1 , Rebaudioside I claim 1 , Rebaudioside J claim 1 , Rebaudioside K claim 1 , Rebaudioside L claim 1 , Rebaudioside M claim 1 , Rebaudioside N claim 1 , Rebaudioside O claim 1 , Rebaudioside Q claim 1 , Stevioside claim 1 , Steviolbioside claim 1 , Dulcoside A claim 1 , Rubusoside claim 1 , or other glycoside of steviol and combinations thereof.3. The method of wherein the water and powder composition comprising at least one steviol glycoside ratio is 10:1 (w/w).4. The method of wherein the water and powder composition comprising at least one steviol glycoside is heated to 80-100° C.5. The method of wherein solution is cooled down to −24° C. to −196° C. to obtain solidified solution.6. The method of wherein solidified solution is dried by a freeze drying apparatus.7. The method of claim 1 , wherein the highly soluble steviol glycoside composition solubility in water is about 2 grams per 100 grams of water.8. A highly soluble steviol glycoside composition powder ...

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Номер записи: 22
16-01-2020 дата публикации

Compositions and Methods for Improving Rebaudioside M Solubility

Номер: US20200015505A1
Автор: Chen Youlung, Ma Gil, MARKOSYAN Avetik, Prakash Indra
Принадлежит:

Rebaudioside M compositions with improved aqueous solubility and methods for preparing the same are provided herein. The rebaudioside M compositions include (i) disordered crystalline compositions comprising rebaudioside M and rebaudioside D, (ii) spray-dried compositions comprising rebaudioside M, rebaudioside D and steviol glycoside mixtures and/or rebaudioside B and/or NSF-02, (iii) spray-dried compositions comprising rebaudioside M, rebaudioside D and at least one surfactant, polymer, saponin, carbohydrate, polyol, preservative or a combination thereof. Sweetened compositions, such a beverages, containing the rebaudioside M compositions with improved water solubility are also provided herein. 128.-. (canceled)29. A beverage comprising a composition comprising (i) rebaudioside M , (ii) rebaudioside D and (iii) a substance selected from the group consisting of a second steviol glycoside mixture , rebaudioside B , glucosylated steviol glycosides and a combination thereof , whereina. rebaudioside M and rebaudioside D are provided as a first steviol glycoside mixture, wherein rebaudioside M is present in the first steviol glycoside mixture in an amount from about 75% to about 90% by weight and rebaudioside D is present in the first steviol glycoside mixture in an amount from about 5% to about 25% by weight;b. the composition is spray-dried prior to dissolution in the beverage; andc. the composition has a water solubility of about 0.3% (w/v) or greater.30. The beverage of claim 29 , wherein the beverage is selected from a mid-calorie claim 29 , low-calorie and zero-calorie beverage.31. The beverage of claim 29 , wherein the beverage is a carbonated beverage.32. The beverage of claim 31 , wherein the carbonated beverage is selected from the group consisting of a cola claim 31 , lemon-lime flavored sparkling beverage claim 31 , orange-flavored sparkling beverage claim 31 , grape-flavored sparkling beverage claim 31 , strawberry-flavored sparkling beverage claim 31 , ...

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Номер записи: 23
17-01-2019 дата публикации

PURIFIED REBAUDIOSIDE A

Номер: US20190016747A1
Автор: CHASE Robert Gordon, FRANCIS Gordon James, Jackson Mel Clinton
Принадлежит:

The invention provides a high throughput, high purity, high yield system and method of isolating and purifying rebaudioside A (“Reb A”), with acceptable water solubility for all commercial uses, from commercially available starting material. The invention also provides a means of maximizing yields of 99+% purity Reb A based on the attributes of a given batch of starting material. The purity of the Reb A final product, up to 99+% purity, can be selected based on a starting material assay. 1. An alcohol adduct of purified rebaudioside A , that is devoid of bitterness or aftertaste.2. The alcohol adduct of claim 1 , wherein the alcohol adduct is obtained by resolving purified rebaudioside A in an alcohol solvent; the alcohol solvent contains water and alcohol.3. The alcohol adduct of claim 2 , wherein the alcohol is selected from the group consisting of methanol claim 2 , ethanol claim 2 , propanol claim 2 , isopropanol claim 2 , n-butanol claim 2 , sec-butanol claim 2 , isobutanol claim 2 , tert-butanol claim 2 , n-pentanol claim 2 , sec-pentanol claim 2 , isopentanol claim 2 , and neopentanol.4. The alcohol adduct of claim 3 , wherein claim 3 , the alcohol is ethanol.5. The alcohol adduct of claim 1 , wherein claim 1 , the alcohol adduct is in crystal form.6. The alcohol adduct of claim 5 , characterized in that claim 5 , there are diffraction peaks at 20 values of about 4.6 claim 5 , 8.9 claim 5 , 11.9 claim 5 , 13.5 claim 5 , 14.0 claim 5 , 15.2 and 19.1 in a PXRD pattern.7. The alcohol adduct of claim 1 , wherein claim 1 , the alcohol adduct has a melting point of from about 242° C. to about 244° C.8. A product comprising:{'claim-ref': {'@idref': 'CLM-00001', 'claim 1'}, 'a sweetener composition comprising at least 95% by weight on a dry basis of the alcohol adduct of , wherein the product is selected from the group consisting of food, beverage, pharmaceutical composition, tobacco, nutraceutical, oral hygienic composition, or cosmetic.'}9. The product according to ...

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Номер записи: 24
16-01-2020 дата публикации

PRODUCTION OF STEVIOL GLYCOSIDE IN RECOMBINANT HOSTS

Номер: US20200017896A1
Автор: Carlsen Simon, Chuang Lim Swee, Dalgaard Mikkelsen Michael, Douchin Veronique, Düring Louis, Olsson Kim, Simon Ernesto
Принадлежит:

The invention relates to recombinant microorganisms and methods for producing steviol glycosides, glycosylated ent-kaurenol, and glycosylated ent-kaurenoic acid. 1. An in vitro method for producing a steviol glycoside , a glycosylated ent-kaurenol compound , and/or a glycosylated ent-kaurenoic acid compound , comprising: a first polypeptide capable of catalyzing beta 1,2 glycosylation of the C2′ of the 13-O-glucose, 19-O-glucose, or both 13-O-glucose and 19-O-glucose of the steviol glycoside and having 90% or greater sequence identity to the amino acid sequence set forth in SEQ ID NO:11;', '(ii) a second polypeptide capable of catalyzing beta 1,2 glycosylation of the C2′ of the 13-O-glucose, 19-O-glucose, or both 13-O-glucose and 19-O-glucose and having 70% or greater sequence identity to the amino acid sequence set forth in SEQ ID NO:17 or 18;', '(iii) a polypeptide capable of capable of catalyzing glycosylation of steviol or the steviol glycoside at its C-13 hydroxyl group and having 55% or greater sequence identity to the amino acid sequence set forth in SEQ ID NO:7; and/or', '(iv) a polypeptide capable of capable of beta 1,3 glycosylation of the C3′ of the 13-O-glucose, 19-O-glucose, or both 13-O-glucose and 19-O-glucose of the steviol glycoside having 50% or greater sequence identity to the amino acid sequence set forth in SEQ ID NO:9;, '(a) adding one or more of 'wherein at least one of the polypeptides is a recombinant polypeptide; and', 'and plant-derived- or synthetic steviol or steviol glycosides to a reaction mixture;'}(b) synthesizing steviol glycoside, glycosylated ent-kaurenol compound, and/or the glycosylated ent-kaurenoic acid compound in the reaction mixture.2. The method of claim 1 , further comprising:(c) recovering the steviol glycoside, glycosylated ent-kaurenol compound, and/or a glycosylated ent-kaurenoic acid compound alone or a composition comprising the steviol glycoside, glycosylated ent-kaurenol compound, and/or the glycosylated ent- ...

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Номер записи: 25
10-02-2022 дата публикации

SWEETNESS ENHANCING METHOD AND APPARATUS FOR INFILTRATING STEVIOSIDE OR FRAGRANT INGREDIENT INTO FRUIT OR VEGETABLE BY USING HIGH-VOLTAGE PULSED ELECTRIC FIELD

Номер: US20220039439A1
Автор: Kim Myoung Yeon
Принадлежит:

The present disclosure relates to a technical idea of infiltrating stevioside or a fragrant ingredient into a fruit or vegetable by using a high-voltage pulsed electric field, and a method for enhancing the sweetness according to an embodiment may comprise the steps of maintaining high-voltage pulse device electrodes installed on at least two sides in a container containing a solution in which a natural sugar or a substance for fragrance is dissolved, injecting an object of the fruit or vegetable between the high-voltage pulse device electrodes, receiving an alternating current from the high-voltage pulse device electrodes to convert the alternating current into a high-voltage direct current, and storing the high-voltage direct current in a capacitor, which is an energy storage device, to charge energy, generating a high-voltage pulsed electric field (PEF) by discharging the charged energy from the high-voltage pulse device electrodes using a pulse generating circuit when the injected object is sensed, forming a potential difference in cell membranes of the object with the generated high-voltage pulsed electric field, and allowing the solution to permeate between the generated perforations of the cell membranes by maintaining the state in which the potential difference is formed for a predetermined time until perforations are generated in the cell membranes of the object. 1. A sweetness enhancing method for infiltrating stevioside into an object without damaging the surface of the object , the sweetness enhancing method comprising the steps of:maintaining high-voltage pulse device electrodes installed on at least two sides in a container containing a solution in which a natural sugar or a substance for fragrance is dissolved;injecting an object of a fruit or vegetable between the high-voltage pulse device electrodes;receiving an alternating current from the high-voltage pulse device electrodes to convert the alternating current into a high-voltage direct current, ...

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Номер записи: 26
28-01-2021 дата публикации

Microbial production of steviol glycosides

Номер: US20210022373A1
Автор: Ajikumar Parayil Kumaran, Jason Donald, Krishna PATEL, Liwei Li, Ryan LIM, Ryan PHILIPPE, Swati Gupta
Принадлежит: Manus Bio Inc

The invention provides methods for making steviol glycosides, including RebM and glycosylation products that are minor products in stevia leaves, and provides enzymes, encoding polynucleotides, and host cells for use in these methods. The invention provides engineered enzymes and engineered host cells for producing steviol glycosylation products, such as RebM, at high purity and/or yield. The invention further provides methods of making products containing steviol glycosides, such as RebM, including food products, beverages, oral care products, sweeteners, and flavoring products.

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Номер записи: 27
10-02-2022 дата публикации

BIOSYNTHESIS PRODUCTION OF STEVIOL GLYCOSIDES AND PROCESSES THEREFORE

Номер: US20220042060A1
Автор: Batten Michael, Byun David, Luo Yang, Mao Guohong, Vick Jacob Edward, Wu Yilin, Yu Xiaodan, Zhang Beihua
Принадлежит: Conagen Inc.

The present invention relates to the production of steviol glycoside rebaudiosides D4, WB1 and WB2 and the production of rebaudioside M from Reb D4. 154.-. (canceled)56Pichia pastoris. The reaction mixture of claim 55 , wherein the beta-glucosidase is a beta-glucosidase.57. The reaction mixture of claim 55 , wherein the beta-glucosidase comprises an amino acid sequence that is at least 90% identical to the amino acid sequence of SEQ ID NO: 5.58. The reaction mixture of claim 55 , wherein the beta-glucosidase comprises the amino acid sequence of SEQ ID NO: 5.59. The reaction mixture of claim 55 , wherein the polynucleotide comprises a nucleotide sequence that is at least 90% identical to the nucleotide sequence of SEQ ID NO: 6.60. The reaction mixture of claim 55 , wherein the polynucleotide comprises the nucleotide sequence of SEQ ID NO: 6.61. The reaction mixture of claim 55 , wherein the cell is a yeast cell.62. The reaction mixture of claim 55 , wherein the cell is a bacterial cell.63. The reaction mixture of claim 55 , wherein the cell is a plant cell.65. The reaction mixture of claim 64 , wherein the UDP glycosyltransferase comprises an amino acid sequence that is at least 90% identical to the amino acid sequence of SEQ ID NO: 9.66. The reaction mixture of claim 64 , wherein the UDP glycosyltransferase comprises the amino acid sequence of SEQ ID NO: 9.67. The reaction mixture of claim 64 , wherein the polynucleotide comprises a nucleotide sequence that is at least 90% identical to the nucleotide sequence of SEQ ID NO: 10.68. The reaction mixture of wherein the polynucleotide comprises the nucleotide sequence of SEQ ID NO: 10.69. The reaction mixture of claim 64 , wherein the cell is a yeast cell.70. The reaction mixture of claim 64 , wherein the cell is a bacterial cell.71. The reaction mixture of claim 64 , wherein the cell is a plant cell.72. The reaction mixture of claim 64 , further comprising a substrate selected from the group consisting of: sucrose claim ...

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Номер записи: 28
29-01-2015 дата публикации

DITERPENE PRODUCTION

Номер: US20150031868A1
Автор: BOER Viktor Marius, Kumar Manoj, LEHMANN Martin, Meijrink Bernard, SAGT CORNELIS MARIA JACOBUS, Trueheart Joshua, VAN DEN BERG Marco Alexander, Wu Liang, Zwart Jens Priscilla
Принадлежит:

The present invention relates to a recombinant microorganism comprising one or more nucleotide sequence(s) encoding: a polypeptide having ent-copalyl pyrophosphate synthase activity; a polypeptide having ent-Kaurene synthase activity; a polypeptide having ent-Kaurene oxidase activity; and a polypeptide having kaurenoic acid 13-hydroxylase activity, whereby expression of the nucleotide sequence(s) confer(s) on the microorganism the ability to produce at least steviol. The recombinant microorganism may also be capable of expressing one or more UDP-glucosyltransferases such that the microorganism is capable of producing one or more steviol glycosides. 1. A recombinant microorganism comprising at least one nucleotide sequence encoding:a polypeptide having ent-copalyl pyrophosphate synthase activity;a polypeptide having ent-Kaurene synthase activity;a polypeptide having ent-Kaurene oxidase activity; anda polypeptide having kaurenoic acid 13-hydroxylase activity,whereby expression of the nucleotide sequence confers on the microorganism an ability to produce at least steviol.2. A recombinant microorganism according to claim 1 , wherein the microorganism comprises one or more nucleotide sequences encoding a polypeptide having UDP-glucosyltransferase activity claim 1 ,whereby expression of the nucleotide sequence confers on the microorganism an ability to produce at least one of steviolmonoside, steviolbioside, stevioside or rebaudioside A, rebaudioside B, rebaudioside C, rebaudioside D, rebaudioside E, rebaudioside F, rubusoside, dulcoside A.3. A recombinant microorganism according to claim 2 , wherein the microorganism comprises a nucleotide sequence encoding a polypeptide capable of catalyzing an addition of a C-13-glucose to steviol claim 2 ,whereby expression of the nucleotide sequence confers on the microorganism an ability to produce at least steviolmonoside.4. A recombinant microorganism according to claim 2 , wherein the microorganism comprises a nucleotide sequence ...

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Номер записи: 29
29-01-2015 дата публикации

HIGH-PURITY STEVIOL GLYCOSIDES

Номер: US20150031869A1
Автор: JARRIN Cyrille, MARKOSYAN Avetik, PRAKASH CHATURVEDULA Venkata Sai, Prakash Indra, ROBE Patrick, ter Halle Rob
Принадлежит:

Methods of preparing highly purified steviol glycosides, particularly rebaudiosides A, D and X are described. The method includes expression of UDP-glucosyltransferases from Bertoni, which are capable converting certain steviol glycosides to rebaudiosides A, D and X. The highly purified rebaudiosides A, D and X, are useful as non-caloric sweetener in edible and chewable compositions such as any beverages, confectioneries, bakery products, cookies, and chewing gums. 126-. (canceled)27. A method for making Rebaudioside X comprising converting Rebaudioside D to Rebaudioside X using a UDP-glucosyltransferase.28. Rebaudioside X made by the method of and having a purity of greater than about 80% by weight.29. Rebaudioside X made by the method of and having a purity of greater than about 90% by weight.30. Rebaudioside X made by the method of and having a purity of greater than about 95% by weight.31. A consumable product comprising the Rebaudioside X made by the method of .32. The method of claim 27 , wherein the UDP-glucosyltransferase comprises any UDP-glucosyltransferase capable of adding at least one glucose unit to Rebaudioside D to form Rebaudioside X.33. The method of claim 32 , wherein the UDP-glucosyltransferase is capable of adding a glucose unit to a C-19 position of Rebaudioside D. The present invention is a continuation application of PCT Application No. PCT/US2013/030439, filed Mar. 12, 2013, which claims the benefit of U.S. Provisional Application No. 61/649,978, filed May 22, 2013. The contents of each of these applications are incorporated herein by reference in their entireties.The present invention relates to a biocatalytic process for preparing compositions comprising steviol glycosides, including highly purified steviol glycoside compositions.The text file entitled “PureCircle 22PCT ST25.txt,” created on Mar. 12, 2013, having 5 KB (kilobytes) of data, and filed concurrently herewith, is hereby incorporated by reference in its entirety in this ...

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Номер записи: 30
04-02-2021 дата публикации

Prebiotic Compositions And Methods Of Production Thereof

Номер: US20210030039A1
Автор: Oswaldo HERNANDEZ, Sofia Kolida, Stephen Patrick O'HARA
Принадлежит: Optibiotix Ltd

The present invention relates to a prebiotic composition comprising: (i) a enzymatically modified high intensity sweetener glycoside; and an oligosaccharide. In particular, the invention relates to a galactosylated and/or fructosylated and/or deglycosylated high intensity sweetener glycoside; and (ii) an oligosaccharide obtained during the same enzymatic reaction. Uses and methods of producing the composition are also described.

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Номер записи: 31
30-01-2020 дата публикации

ENGINEERED GLYCOSYLTRANSFERASES AND STEVIOL GLYCOSIDE GLUCOSYLATION METHODS

Номер: US20200032227A1
Автор: Dellas Nikki, Entwistle David, Galanie Stephanie Sue, Liang Jack, Liu Joyce, Mayo Melissa Ann, Vroom Jonathan
Принадлежит:

The present invention provides engineered glycosyltransferase (GT) enzymes, polypeptides having GT activity, and polynucleotides encoding these enzymes, as well as vectors and host cells comprising these polynucleotides and polypeptides. The present invention provides engineered sucrose synthase (SuS) enzymes, polypeptides having SuS activity, and polynucleotides encoding these enzymes, as well as vectors and host cells comprising these polynucleotides and polypeptides. The present invention also provides compositions comprising the GT enzymes and methods of using the engineered GT enzymes to make products with β-glucose linkages. The present invention further provides compositions and methods for the production of rebaudiosides (e.g., rebaudioside M, rebaudioside A, rebaudioside I, and rebaudioside D). The present invention also provides compositions comprising the SuS enzymes and methods of using them. Methods for producing GT and SuS enzymes are also provided. 1. An engineered glycosyltransferase comprising a polypeptide sequence that has at least 90% , 91% , 92% , 93% , 94% , 95% , 96% , 97% , 98% , 99% , or more sequence identity to SEQ ID NO: 2.2. The engineered glycosyltransferase of claim 1 , wherein said engineered glycosyltransferase comprising a polypeptide that is at least 95% claim 1 , 96% claim 1 , 97% claim 1 , 98% claim 1 , 99% claim 1 , or more sequence identity to SEQ ID NO: 20.3. The engineered glycosyltransferase of claim 2 , wherein said polypeptide sequence of said engineered glycosyltransferase comprises at least one mutation or mutation set at one or more positions selected from 41/72/233/338 claim 2 , 41/72/338 claim 2 , 41/144/233 claim 2 , 41/233 claim 2 , 41/233/338 claim 2 , 61 claim 2 , 61/87/91/107 claim 2 , 61/87/91/259 claim 2 , 61/91/431 claim 2 , 61/107 claim 2 , 61/259/428 claim 2 , 61/407/428 claim 2 , 61/411 claim 2 , 72 claim 2 , 72/76 claim 2 , 72/76/163/197 claim 2 , 72/76/195/233 claim 2 , 72/76/197/204 claim 2 , 72/76/207/ ...

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Номер записи: 32
04-02-2021 дата публикации

DOXA PROTEIN MUTANT, AND CODING GENE AND APPLICATIONS THEREOF

Номер: US20210032606A1
Автор: Han Shuang, He Yingying, Jiang Xing, ZHENG Linghui, Zhou Min
Принадлежит:

The present invention relates to a DoxA protein mutant having an amino acid sequence set forth in SEQ ID No. 16, and coding gene thereof. The protein mutant or the coding gene thereof can be used for producing epirubicin. The present invention further relates to a capable of efficiently expressing epirubicin, which is constructed by replacing the dnmV gene of a starting in situ with the avrE gene and mutating the doxA gene of the starting into a gene encoding the protein set forth in SEQ ID No. 16. The fermentation broth of this has an epirubicin potency of up to 102.0 μg/ml. 1. A protein comprising the amino acid sequence set forth in SEQ ID No. 16.2. A biological material related to the protein according to claim 1 , which is the following B1) or B2):{'claim-ref': {'@idref': 'CLM-00001', 'claim 1'}, 'B1) nucleic acid molecule encoding the protein according to ;'}B2) expression cassette, recombinant vector, recombinant microorganism or transgenic cell line comprising the nucleic acid molecule of B1).3. The biological material according to claim 2 , wherein the nucleic acid molecule of B1) is a gene represented by the following 1) or 2) or 3):1) a DNA molecule set forth in SEQ ID No. 15;{'claim-ref': {'@idref': 'CLM-00001', 'claim 1'}, '2) a DNA molecule that hybridizes to the DNA molecule as defined in 1) under stringent conditions and encodes the protein according to ;'}{'claim-ref': {'@idref': 'CLM-00001', 'claim 1'}, '3) a DNA molecule having 90% or more identity to the DNA molecule as defined in 1) or 2) and encoding the protein according to .'}4StreptomycesStreptomycesStreptomyces. A method for constructing epirubicin-expressing claim 2 , comprising the steps of: replacing the dnmV gene of a starting in situ with avrE gene claim 2 , and mutating the doxA gene of the starting into a gene sequence encoding the protein set forth in SEQ ID No. 16.5Streptomyces. The method according to claim 4 , wherein the preservation number of the starting is CGMCC No. 48276. ...

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Номер записи: 33
09-02-2017 дата публикации

GLUCOSYL STEVIA COMPOSITION

Номер: US20170035086A1
Автор: MARKOSYAN Avetik
Принадлежит: PureCircle Sdn Bhd

Glucosyl compositions are prepared from steviol glycosides of Bertoni. The glucosylation was performed by cyclodextrin glucanotransferase using the starch as source of glucose residues. The compositions mainly comprise glucosyl derivatives with superior taste characteristics and can be used as sweetness enhancers, flavor enhancers and sweeteners in foods, beverages, cosmetics and pharmaceuticals. 113-. (canceled)14SteviaStevia rebaudiana. The food claim 17 , beverage claim 17 , cosmetic or pharmaceutical product of claim 17 , further comprising an additional sweetening agent selected from the group consisting of: extract claim 17 , steviol glycosides claim 17 , stevioside claim 17 , rebaudioside A claim 17 , rebaudioside B claim 17 , rebaudioside C claim 17 , rebaudioside D claim 17 , rebaudioside E claim 17 , rebaudioside F claim 17 , dulcoside A claim 17 , steviolbioside claim 17 , rubusoside claim 17 , other steviol glycosides found in Bertoni plant and mixtures thereof claim 17 , Luo Han Guo extract claim 17 , mogrosides claim 17 , high-fructose corn syrup claim 17 , corn syrup claim 17 , invert sugar claim 17 , fructooligosaccharides claim 17 , inulin claim 17 , inulooligosaccharides claim 17 , coupling sugar claim 17 , maltooligosaccharides claim 17 , maltodextins claim 17 , corn syrup solids claim 17 , glucose claim 17 , maltose claim 17 , sucrose claim 17 , lactose claim 17 , aspartame claim 17 , saccharin claim 17 , sucralose claim 17 , sugar alcohols claim 17 , and a combination thereof.15. The food claim 17 , beverage claim 17 , cosmetic or pharmaceutical product of claim 17 , further comprising an additional flavoring agent selected from the group consisting of: lemon claim 17 , orange claim 17 , fruity claim 17 , banana claim 17 , grape claim 17 , pear claim 17 , pineapple claim 17 , mango claim 17 , bitter almond claim 17 , cola claim 17 , cinnamon claim 17 , sugar claim 17 , cotton candy claim 17 , vanilla claim 17 , and a combination thereof.16. The ...

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Номер записи: 34
08-02-2018 дата публикации

NON-CALORIC SWEETENER

Номер: US20180037600A1
Автор: CHATURVEDULA VENKATA SAI PRAKASH, Mao Guohong, Yu Xiaodan
Принадлежит: Conagen Inc.

Disclosed is a steviol glycoside referred to as rebaudioside D2. Rebaudioside D2 has five β-D-glucosyl units connected to the aglycone steviol. Also disclosed are methods for producing rebaudioside D2, a UDP-glycosyltransferase fusion enzyme, and methods for producing rebaudioside D and rebaudioside E. 132-. (canceled)33. A method for synthesizing rebaudioside D , the method comprising:preparing a reaction mixture comprising rebaudioside A; a substrate selected from the group consisting of sucrose, uridine diphosphate (UDP) and uridine diphosphate-glucose (UDP-glucose); and a UDP-glycosyltransferase selected from the group consisting of a uridine diphospho glycosyltransferase and a UDP-glycosyltransferase fusion enzyme comprising a uridine diphospho glycosyltransferase domain coupled to a sucrose synthase domain; and incubating the reaction mixture for a sufficient time to produce rebaudioside D, wherein a glucose is covalently coupled to the rebaudioside A to produce rebaudioside D.34Oryza sativa. The method of claim 33 , wherein the UDP-glycosyltransferase is a uridine diphospho glycosyltransferase EUGT11.35. The method of further comprising adding a sucrose synthase to the reaction mixture.36ArabidopsisCoffeaStevia. The method of claim 35 , wherein the sucrose synthase is selected from the group consisting of an sucrose synthase 1; a sucrose synthase 1 and a sucrose synthase 1.37Arabidopsis thaliana. The method of claim 35 , wherein the sucrose synthase is an sucrose synthase 1.38. The method of claim 33 , wherein the UDP-glycosyltransferase is a UDP-glycosyltransferase fusion enzyme comprising a uridine diphospho glycosyltransferase domain coupled to a sucrose synthase domain.39. The method of claim 38 , wherein the UDP-glycosyltransferase fusion enzyme comprises an amino acid sequence having about 90% sequence identity to SEQ ID NO:5.40Oryza sativa. The method of claim 33 , wherein the uridine diphospho glycosyltransferase domain is an uridine diphospho ...

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Номер записи: 35
07-02-2019 дата публикации

High-Purity Steviol Glycosides

Номер: US20190040434A1
Автор: Badie Aurelien, Bunders Cynthia, Cyrille Jarrin, Halle Rober Ter, MARKOSYAN Avetik, Prakash Indra, Soni Pankaj
Принадлежит:

Methods of preparing highly purified steviol glycosides, particularly rebaudiosides A, D and M are described. The methods include utilizing recombinant microorganisms for converting various staring compositions to target steviol glycosides. In addition, novel steviol glycosides reb D2 and reb M2 are disclosed, as are methods of preparing the same. The highly purified rebaudiosides are useful as non-caloric sweetener in edible and chewable compositions such as any beverages, confectioneries, bakery products, cookies, and chewing gums. 1. A method for producing target steviol glycosides , comprising the steps of:a. providing a starting composition comprising an organic compound with at least one carbon atom;b. providing a microorganism or a biocatalyst containing at least one enzyme selected from steviol biosynthesis enzymes, UDP-glycosyltransferases, and optionally providing UDP-glucose recycling enzymes;c. contacting the microorganism with a medium containing the starting composition to produce a medium comprising at least one target steviol glycoside.2. The method of claim 1 , further comprising the step of:d. purifying the target steviol glycoside from the medium to provide a highly purified target steviol glycoside composition.3. The method of claim 1 , wherein the organic compound is selected from the group consisting of polyols claim 1 , carbohydrates claim 1 , steviol glycosides and combinations thereof.4. The method of claim 1 , wherein the biocatalyst is an enzyme claim 1 , or a cell comprising one or more enzymes claim 1 , capable of converting the organic compound to target steviol glycoside.5. The method of claim 1 , wherein the target steviol glycoside is selected from the group consisting of stevioside claim 1 , reb A claim 1 , reb D claim 1 , reb D2 claim 1 , reb E claim 1 , reb M claim 1 , reb M2 and mixtures thereof.6. The method of claim 1 , wherein the enzyme is selected from the group consisting of geranylgeranyl diphosphate synthase claim 1 , ...

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Номер записи: 36
18-02-2016 дата публикации

GLUCURONOSYLTRANSFERASE, GENE ENCODING SAME AND USE THEREOF

Номер: US20160046912A1
Автор: MURANAKA Toshiya, Ohyama Kiyoshi, SAITO Kazuki, Seki Hikaru
Принадлежит: RIKEN

Provided are an enzyme involved in a glycyrrhizin biosynthetic system, a gene of the enzyme and use thereof in order to stably and continuously provide a large amount of glycyrrhizin. Glucuronosyltransferase with an activity of further transferring glucuronic acid to the hydroxy group at the 2-position of glucuronic acid in an oleanane-type triterpenoid monoglucuronide is identified to provide the transferase, a gene for the transferase and use thereof. 1. A polypeptide having an activity to transfer glucuronic acid to the hydroxy group at the 2-position of glucuronic acid in an oleanane-type triterpenoid monoglucuronide.2. The polypeptide according to claim 1 , wherein the oleanane-type triterpenoid monoglucuronide is selected from the group consisting of β-amyrin monoglucuronide claim 1 , 11-oxo-β-amyrin monoglucuronide claim 1 , 30-hydroxy-11-oxo-β-amyrin monoglucuronide claim 1 , 30-hydroxy-β-amyrin monoglucuronide claim 1 , 11-oxoglycyrrhetinic acid monoglucuronide and glycyrrhetinic acid monoglucuronide.3GlycyrrhizaMedicago. The polypeptide according to claim 1 , derived from a plant or a plant.4GlycyrrhizaG. uralensis.. The polypeptide according to claim 3 , wherein the plant is5. The polypeptide according to claim 1 , comprising an amino acid sequence represented by SEQ ID NO: 3.6. The polypeptide according to claim 1 , comprising any of amino acid sequences of the following (a) to (c):(a) an amino acid sequence represented by SEQ ID NO: 4,(b) an amino acid sequence derived from the amino acid sequence represented by SEQ ID NO: 4 by deletion, replacement or addition of one or several amino acids, and or(c) an amino acid sequence having 80% or more identity with the amino acid sequence represented by SEQ ID NO: 4.7. A polynucleotide encoding the polypeptide according to .8. The polynucleotide according to claim 7 , comprising any of nucleotide sequences of the following (d) to (g):(d) a nucleotide sequence represented by SEQ ID NO: 5,(e) a nucleotide sequence ...

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Номер записи: 37
15-02-2018 дата публикации

UDP-GLYCOSYLTRANSFERASES

Номер: US20180044645A1
Автор: BOER Viktor Marius, KOLEN Catharina Petronella Antonia Maria, VAN LEEUWEN Johannes Gustaaf Ernst, ZWARTJENS Priscilla
Принадлежит:

The present invention relates to a recombinant host comprising a recombinant nucleic acid sequence encoding a polypeptide having at least about: a. 85% identity to the amino acid sequence set forth in SEQ ID NO: 1; b. 85% identity to the amino acid sequence set forth in SEQ ID NO: 3; c. 85% identity to the amino acid sequence set forth in SEQ ID NO: 6; d. 85% identity to the amino acid sequence set forth in SEQ ID NO: 9; e. 85% identity to the amino acid sequence set forth in SEQ ID NO: 11; f. 85% identity to the amino acid sequence set forth in SEQ ID NO: 14; g. 85% identity to the amino acid sequence set forth in SEQ ID NO: 17; h. 85% identity to the amino acid sequence set forth in SEQ ID NO: 20; i. 85% identity to the amino acid sequence set forth in SEQ ID NO: 22; or j. 85% identity to the amino acid sequence set forth in SEQ ID NO: 25. 1. A recombinant host comprising a recombinant nucleic acid sequence encoding a polypeptide having at least about:a. 85% identity to the amino acid sequence set forth in SEQ ID NO: 1;b. 85% identity to the amino acid sequence set forth in SEQ ID NO: 3;c. 85% identity to the amino acid sequence set forth in SEQ ID NO: 6;d. 85% identity to the amino acid sequence set forth in SEQ ID NO: 9;e. 85% identity to the amino acid sequence set forth in SEQ ID NO: 11;f. 85% identity to the amino acid sequence set forth in SEQ ID NO: 14;g. 85% identity to the amino acid sequence set forth in SEQ ID NO: 17;h. 85% identity to the amino acid sequence set forth in SEQ ID NO: 20;i. 85% identity to the amino acid sequence set forth in SEQ ID NO: 22; orj. 85% identity to the amino acid sequence set forth in SEQ ID NO: 25.2. The recombinant host according to which is capable of producing a glycosylated diterpene claim 1 , such as a steviol glycoside.3. The recombinant host according to wherein one or more recombinant nucleotide sequence(s) encoding:a polypeptide having ent-copalyl pyrophosphate synthase activity;a polypeptide having ent-Kaurene ...

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Номер записи: 38
15-02-2018 дата публикации

Methods for the Enzymatic Modification of Steviol Glycosides, Modified Steviol Glycosides Obtainable Thereby, and the Use Thereof as Sweeteners

Номер: US20180044708A1
Автор: Dijkhuizen Lubbert, Gerwig Gerrit Johannes, Kamerling Johannis Paulus, te Poele Evelien Maria
Принадлежит:

The present invention relates generally to the production of steviol glycosides. Provided is a method for enzymatically providing a modified steviol glycoside, comprising incubating a steviol glycoside substrate in A the presence of sucrose and the glucansucrase GTF180 of strain 180, or a mutant thereof having the desired transglycosylation activity. Also provided are modified steviol glycosides obtainable by a method of the invention, and the use thereof as low-glycemic sweetener. 1Lactobacillus reuteri. A method for enzymatically providing a modified steviol glycoside , comprising incubating a steviol glycoside substrate in the presence of sucrose as a glucose donor and the glucansucrase GTF180 of strain 180 , or a mutant thereof having the desired transglycosylation activity.2. The method according to claim 1 , wherein said modified steviol glycoside is modified with at least one glucose residue.3. The method according to claim 1 , wherein said modified steviol glycoside is modified with one or more glucoses via an (α1→6) glycosidic bond claim 1 , an (α 1→3) glycosidic bond claim 1 , or a combination thereof.4. The method according to claim 1 , wherein said modified steviol glycoside is modified at the C-13 and/or the C-19 site of the steviol glycoside.5. The method according to claim 4 , wherein said modified steviol glycoside is only modified at the C-19 site.6. The method according to claim 5 , wherein said modified steviol glycoside is only modified with a single glucose residue at the C-19 site.7. The method according to claim 1 , wherein said steviol glycoside substrate is a steviol glycoside.8. The method according to claim 7 , wherein said steviol glycoside is Rebaudioside A [13-({β-D-glucopyranosyl-(1→2)-[β-D-glucopyranosyl-(1→3)-]β-D-glucopyranosyl}oxy)ent-kaur-16-en-19-oic acid α-D-glucopyranosyl ester] claim 7 , or Stevioside (13-{[β-D-glucopyranosyl-(1→2)-(3-D-glucopyranosyl]oxy}ent-kaur-16-en-19-oic acid α-D-glucopyranosyl ester)9. The method ...

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Номер записи: 39
13-02-2020 дата публикации

STEVIA EXTRACTS

Номер: US20200045998A1
Автор: Bu Yu Cheng, Li Shan Wang, MARKOSYAN Avetik
Принадлежит:

A extract made from leaves of the plant is described. The extract has desired levels of steviol glycosides and is useful in food, beverage, and other consumable products. 14-. (canceled)5Stevia rebaudiana. Leaves of a cultivar , comprising Rebaudioside D , Rebaudioside M , Rebaudioside N , and Rebaudioside O , having a total steviol glycosides content defined as the sum of the concentrations of Rebaudioside A , Rebaudioside B , Rebaudioside C , Rebaudioside D , Rebaudioside E , Rebaudioside F , Rebaudioside M , Rebaudioside N , Rebaudioside O , Stevioside , Steviolbioside , Dulcoside A and Rubusoside on a wt/wt dry basis , wherein a relative concentration of Rebaudioside M to the total steviol glycosides content is greater than 1.4% , and wherein the sum of the relative concentrations of Rebaudioside D , Rebaudioside E , Rebaudioside N and Rebaudioside O to the total steviol glycosides content is greater than 5.8% , wherein the relative concentration is calculated as follows:{'br': None, 'Relative concentration of steviol glycoside=[concentration of steviol glycoside (% wt/wt on dry basis)/total steviol glycosides content (% wt/wt on dry basis)]×100.'}6. The leaves of claim 5 , wherein the relative concentration of Rebaudioside D is at least 2.4%7. The leaves of claim 5 , wherein the relative concentration of Rebaudioside N is at least 1.6%.8. The leaves of claim 5 , wherein the relative concentration of Rebaudioside E is at least 1.0%.9. The leaves of claim 5 , wherein the relative concentration of Rebaudioside O is at least 0.8%.10. An aqueous extract of the leaves of claim 5 , made by a process comprising the steps of:{'i': Stevia rebaudiana', 'Stevia rebaudiana, 'a) providing leaves, wherein leaves comprise Rebaudioside M at a relative concentration greater than 1.4%;'}b) providing a solvent comprising water;{'i': 'Stevia rebaudiana', 'c) contacting the leaves with the solvent to extract the steviol glycoside from the leaves; and'}{'i': Stevia rebaudiana', ' ...

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Номер записи: 40
14-02-2019 дата публикации

PRODUCTION OF STEVIOL GLYCOSIDES IN RECOMBINANT HOSTS

Номер: US20190048356A1
Автор: Douchin Veronique, Takos Adam Matthew
Принадлежит:

The invention relates to recombinant microorganisms and methods for producing steviol glycosides and steviol glycoside precursors. 1. A recombinant host cell capable of producing one or more steviol glycosides or a steviol glycoside composition in a cell culture , comprising a recombinant gene encoding damage resistance protein 1 (DAP1) polypeptide having at least 60% sequence identity to the amino acid sequence set forth in SEQ ID NO:2;wherein expression of the recombinant gene encoding DAP1 polypeptide results in increased production of the one or more steviol glycosides or the steviol glycoside composition in the cell culture.2. The recombinant host cell of claim 1 , further comprising:(a) a gene encoding a polypeptide capable of synthesizing geranylgeranyl pyrophosphate (GGPP) from farnesyl diphosphate (FPP) and isopentenyl diphosphate (IPP);(b) a gene encoding a polypeptide capable of synthesizing ent-copalyl diphosphate from GGPP;(c) a gene encoding a polypeptide capable of synthesizing ent-kaurene from ent-copalyl pyrophosphate;(d) a gene encoding a polypeptide capable of glycosylating steviol or a steviol glycoside at its C-13 hydroxyl group;(e) a gene encoding a polypeptide capable of beta 1,3 glycosylation of the C3′ of the 13-O-glucose, 19-O-glucose, or both 13-O-glucose and 19-O-glucose of a steviol glycoside;(f) a gene encoding a polypeptide capable of glycosylating steviol or a steviol glycoside at its C-19 carboxyl group; and/or(g) a gene encoding a polypeptide capable of beta 1,2 glycosylation of the C2′ of the 13-O-glucose, 19-O-glucose, or both 13-O-glucose and 19-O-glucose of a steviol glycoside;and 'wherein the polypeptide comprises a polypeptide having at least 70% sequence identity to one of the amino acid sequences set forth in SEQ ID NO:60, SEQ ID NO:62, SEQ ID NO:117, SEQ ID NO:66, SEQ ID NO:68, SEQ ID NO:70, SEQ ID NO:72, SEQ ID NO:74, or SEQ ID NO:76; and', '(h) a gene encoding a polypeptide capable of synthesizing ent-kaurenoic acid, ent- ...

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Номер записи: 41
25-02-2021 дата публикации

ENGINEERED GLYCOSYLTRANSFERASES AND STEVIOL GLYCOSIDE GLUCOSYLATION METHODS

Номер: US20210054348A1
Автор: Dellas Nikki, Entwistle David, Galanie Stephanie Sue, Liang Jack, Liu Joyce, Moffett Courtney Dianne, Vroom Jonathan
Принадлежит:

The present invention provides engineered glycosyltransferase (GT) enzymes, polypeptides having GT activity, and polynucleotides encoding these enzymes, as well as vectors and host cells comprising these polynucleotides and polypeptides. The present invention provides engineered sucrose synthase (SuS) enzymes, polypeptides having SuS activity, and polynucleotides encoding these enzymes, as well as vectors and host cells comprising these polynucleotides and polypeptides. The present invention also provides compositions comprising the GT enzymes and methods of using the engineered GT enzymes to make products with β-glucose linkages. The present invention further provides compositions and methods for the production of rebaudiosides (e.g., rebaudioside M, rebaudioside A, rebaudioside I, and rebaudioside D). The present invention also provides compositions comprising the SuS enzymes and methods of using them. Methods for producing GT and SuS enzymes are also provided. 1. An engineered glycosyltransferase comprising a polypeptide sequence that is at least 60% , 65% , 70% , 75% , 80% , 85% , 86% , 87% , 88% , 89% , 90% , 91% , 92% , 93% , 94% , 95% , 96% , 97% , 98% , 99% , or more sequence identity to SEQ ID NO: 758 , 770 , 792 , 954 , 1002 , 1054 , 2600 , 2718 , 2814 , 2884 , 3016 , 3082 , 3244 , 3502 , 3346 , 3502 , 3696 , 3956 , 4256 , 4550 , 7324 , and 7784.2. The engineered glycosyltransferase of claim 1 , wherein said polypeptide sequence of said engineered glycosyltransferase comprises at least one mutation or mutation set at one or more positions selected from 69/173/175/243/246/354/365/383/399 claim 1 , 69/173/243/383/399 claim 1 , 56/191/354/383/399 claim 1 , 70/225/246/409/413 claim 1 , 70/115/225/409 claim 1 , 70/225/413 claim 1 , 70/225/247 claim 1 , 74/310/396/424 claim 1 , 74/396 claim 1 , and 173/175/191/365/383/399 claim 1 , wherein said positions are numbered with reference to SEQ ID NO:758.3. The engineered glycosyltransferase of claim 1 , wherein said ...

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Номер записи: 42
25-02-2021 дата публикации

ENGINEERED GLYCOSYLTRANSFERASES AND STEVIOL GLYCOSIDE GLUCOSYLATION METHODS

Номер: US20210054349A1
Автор: Dellas Nikki, Entwistle David, Galanie Stephanie Sue, Liang Jack, Liu Joyce, Moffett Courtney Dianne, Vroom Jonathan
Принадлежит:

The present invention provides engineered glycosyltransferase (GT) enzymes, polypeptides having GT activity, and polynucleotides encoding these enzymes, as well as vectors and host cells comprising these polynucleotides and polypeptides. The present invention provides engineered sucrose synthase (SuS) enzymes, polypeptides having SuS activity, and polynucleotides encoding these enzymes, as well as vectors and host cells comprising these polynucleotides and polypeptides. The present invention also provides compositions comprising the GT enzymes and methods of using the engineered GT enzymes to make products with β-glucose linkages. The present invention further provides compositions and methods for the production of rebaudiosides (e.g., rebaudioside M, rebaudioside A, rebaudioside I, and rebaudioside D). The present invention also provides compositions comprising the SuS enzymes and methods of using them. Methods for producing GT and SuS enzymes are also provided. 1. An engineered sucrose synthase comprising a polypeptide sequence that is at least 60% , 65% , 70% , 75% , 80% , 85% , 86% , 87% , 88% , 89% , 90% , 91% , 92% , 93% , 94% , 95% , 96% , 97% , 98% , 99% , or more sequence identity to SEQ ID NO:72 , 74 , 1080 , 1158 , 1222 , 1392 , 1456 , 1582 , 1764 , 1804 , 1840 , 2064 , 2432 , 2510 , 7506 , and/or 8420.2. The engineered sucrose synthase of claim 1 , wherein said polypeptide sequence of said engineered sucrose synthase comprises at least one mutation or mutation set at one or more positions selected from 4/9/349/532 claim 1 , 4/13/113/343/532 claim 1 , 4/13/113/532 claim 1 , 4/33/47/52/343/532 claim 1 , 4/47/52/532 claim 1 , 4/113/532 claim 1 , 4/13/113 claim 1 , 4/13/532 claim 1 , 4/33/113 claim 1 , 4/343 claim 1 , 7 claim 1 , 8 claim 1 , 44 claim 1 , 95 claim 1 , 117/440 claim 1 , 136 claim 1 , 221 claim 1 , 343/532 claim 1 , 440 claim 1 , 444 claim 1 , 478 claim 1 , 532 claim 1 , 583 claim 1 , 611 claim 1 , 615 claim 1 , 615/789 claim 1 , 695 claim 1 , ...

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Номер записи: 43
22-02-2018 дата публикации

NON-CALORIC SWEETENER

Номер: US20180051049A1
Автор: CHATURVEDULA VENKATA SAI PRAKASH, Mao Guohong, Yu Xiaodan
Принадлежит: Conagen Inc

Disclosed is a steviol glycoside referred to as rebaudioside D2. Rebaudioside D2 has five β-D-glucosyl units connected to the aglycone steviol. Also disclosed are methods for producing rebaudioside D2, a UDP-glycosyltransferase fusion enzyme, and methods for producing rebaudioside D and rebaudioside E. 156-. (canceled)58. The orally consumable product of comprising from about 5 ppm to about 100 ppm rebaudioside D2.59. The orally consumable product of claim 57 , wherein the rebaudioside D2 is the only sweetener.60. The orally consumable product of claim 57 , wherein the orally consumable product has a sweetness intensity equivalent to about 1% (w/v-%) to about 4% (w/v-%) sucrose solution.61. The orally consumable product of further comprising at least one additional sweetener.62. The orally consumable product of claim 61 , wherein the at least one additional sweetener is a high intensity sweetener.63. The orally consumable product of claim 61 , wherein the at least one additional sweetener is a natural high intensity sweetener.64stevia. The orally consumable product of claim 61 , wherein the at least one additional sweetener is selected from the group consisting of a extract claim 61 , a steviol glycoside claim 61 , stevioside claim 61 , rebaudioside A claim 61 , rebaudioside B claim 61 , rebaudioside C claim 61 , rebaudioside D claim 61 , rebaudioside E claim 61 , rebaudioside F claim 61 , dulcoside A claim 61 , rubusoside claim 61 , steviolbioside claim 61 , sucrose claim 61 , high fructose corn syrup claim 61 , fructose claim 61 , glucose claim 61 , xylose claim 61 , arabinose claim 61 , rhamnose claim 61 , erythritol claim 61 , xylitol claim 61 , mannitol claim 61 , sorbitol claim 61 , inositol claim 61 , AceK claim 61 , aspartame claim 61 , neotame claim 61 , sucralose claim 61 , saccharine claim 61 , naringin dihydrochalcone (NarDHC) claim 61 , neohesperidin dihydrochalcone (NDHC) claim 61 , rubusoside claim 61 , mogroside IV claim 61 , siamenoside I claim 61 , ...

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Номер записи: 44
10-03-2022 дата публикации

HIGH-PURITY STEVIOL GLYCOSIDES

Номер: US20220071252A1
Автор: JARRIN Cyrille, MARKOSYAN Avetik, PRAKASH CHATURVEDULA Venkata Sai, Prakash Indra, ROBE Patrick, ter Halle Rob
Принадлежит:

Methods of preparing highly purified steviol glycosides, particularly rebaudiosides A, D and X are described. The method includes expression of UDP-glucosyltransferases from Bertoni, which are capable converting certain steviol glycosides to rebaudiosides A, D and X. The highly purified rebaudiosides A, D and X, are useful as non-caloric sweetener in edible and chewable compositions such as any beverages, confectioneries, bakery products, cookies, and chewing gums. 126.-. (canceled)27. A method for adding at least one glucose unit to a steviol glycoside substrate comprising rebaudioside E to provide a target steviol glycoside , comprising contacting the steviol glycoside substrate with a biocatalyst protein enzyme comprising UDP-glucosyltransferase , wherein the target steviol glycoside is Rebaudioside X.28. The method of claim 27 , wherein the biocatalyst protein enzyme is a recombinant protein.29. The method of claim 27 , further comprising the step of purifying the Rebaudioside X to a purity of greater than about 80% by weight.30. The method of claim 27 , further comprising the step of purifying the Rebaudioside X to a purity of greater than about 90% by weight.31. The method of claim 27 , further comprising the step of purifying the Rebaudioside X to a purity of greater than about 95% by weight.32. The method of claim 27 , wherein the UDP-glucosyltransferase is expressed in a host microorganism.33Escherichia coli, Saccharomyces species, AspergillusPischia. The method of claim 32 , wherein the host microorganism is selected from the group consisting of: species claim 32 , and species. This application is a continuation of U.S. patent application Ser. No. 16/694,893, filed Nov. 25, 2019, which is a continuation of U.S. patent application Ser. No. 15/400,325, filed Jan. 6, 2017, now U.S. Pat. No. 10,485,257, which is a continuation of U.S. patent application Ser. No. 14/954,213, now abandoned, which is a divisional of U.S. patent application Ser. No. 14/469,076, ...

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Номер записи: 45
01-03-2018 дата публикации

NON-CALORIC SWEETENERS AND METHODS FOR SYNTHESIZING

Номер: US20180057519A1
Автор: Mao Guohong, Yu Xiaodan
Принадлежит: Conagen Inc.

Disclosed are steviol glycosides referred to as rebaudioside V and rebaudioside W. Also disclosed are methods for producing rebaudioside M (Reb M), rebaudioside G (Reb G), rebaudioside KA (Reb KA), rebaudioside V (Reb V) and rebaudioside (Reb W). 118-. (canceled)19. A method for synthesizing rebaudioside M from rebaudioside D , the method comprising:preparing a reaction mixture comprising rebaudioside D; substrates selected from the group consisting of sucrose, uridine diphosphate (UDP), uridine diphosphate-glucose (UDP-glucose), and combinations thereof; and a uridine dipospho glycosyltransferase (UDP-glycosyltransferase) selected from the group consisting of an UGT76G1, a UDP-glycosyltransferase fusion enzyme, and combinations thereof; andincubating the reaction mixture for a sufficient time to produce rebaudioside M, wherein a glucose is covalently coupled to the rebaudioside D to produce rebaudioside M.20. The method of further comprising adding a sucrose synthase to the reaction mixture.21ArabidopsisArabidopsisVigna radiate. The method of claim 20 , wherein the sucrose synthase is selected from the group consisting of an sucrose synthase 1; an sucrose synthase 3; and a sucrose synthase.22Arabidopsis thaliana. The method of claim 21 , wherein the sucrose synthase is an sucrose synthase 1.23. The method of claim 19 , wherein the UDP-glycosyltransferase fusion enzyme is an UGT76G1 uridine diphospho glycosyltransferase domain coupled to a sucrose synthase domain.24ArabidopsisArabidopsisVigna radiate. The method of claim 23 , wherein the sucrose synthase domain is selected from the group consisting of an sucrose synthase 1; an sucrose synthase 3; and a sucrose synthase.25Arabidopsis thaliana. The method of claim 24 , wherein the sucrose synthase domain is an sucrose synthase 1.2646-. (canceled)47. The method of claim 19 , wherein the reaction mixture further comprises a UDP-glycosyltransferase fusion enzyme having the sequence of SEQ ID NO: 11. This application ...

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Номер записи: 46
01-03-2018 дата публикации

NON-CALORIC SWEETENERS AND METHODS FOR SYNTHESIZING

Номер: US20180057520A1
Автор: Mao Guohong, Yu Xiaodan
Принадлежит: Conagen Inc.

Disclosed are steviol glycosides referred to as rebaudioside V and rebaudioside W. Also disclosed are methods for producing rebaudioside M (Reb M), rebausoside G (Reb G), rebaudioside KA (Reb KA), rebaudioside V (Reb V) and rebaudioside (Reb W). 125-. (canceled)26. A method for synthesizing rebaudioside M from rebaudioside E , the method comprising:preparing a reaction mixture comprising rebaudioside E; substrates selected from the group consisting of sucrose, uridine diphosphate (UDP), uridine diphosphate-glucose (UDP-glucose), and combinations thereof; and a uridine dipospho glycosyltransferase (UDP-glycosyltransferase) selected from the group consisting of an UGT76G1, a UDP-glycosyltransferase fusion enzyme, and combinations thereof; andincubating the reaction mixture for a sufficient time to produce rebaudioside M, wherein a glucose is covalently coupled to the rebaudioside E to produce rebaudioside D, and a glucose is covalently coupled to the rebaudioside D to produce rebaudioside M.27. The method of further comprising adding a sucrose synthase to the reaction mixture.28. The method of claim 27 , wherein the sucrose synthase is selected from the group consisting of an Arabidopsis sucrose synthase 1; an Arabidopsis sucrose synthase 3; and a Vigna radiate sucrose synthase.29. The method of claim 28 , wherein the sucrose synthase is an Arabidopsis thaliana sucrose synthase 1.30. The method of claim 26 , wherein the UDP-glycosyltransferase fusion enzyme is an UGT76G1 uridine diphospho glycosyltransferase domain coupled to a sucrose synthase domain.31ArabidopsisArabidopsisVigna radiate. The method of claim 30 , wherein the sucrose synthase domain is selected from the group consisting of an sucrose synthase 1; an sucrose synthase 3; and a sucrose synthase.32Arabidopsis thaliana. The method of claim 31 , wherein the sucrose synthase domain is an sucrose synthase 1.3346-. (canceled)47. The method of claim 26 , wherein the reaction mixture further comprises sucrose and ...

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Номер записи: 47
01-03-2018 дата публикации

NON-CALORIC SWEETENERS AND METHODS FOR SYNTHESIZING

Номер: US20180057521A1
Автор: Mao Guohong, Yu Xiaodan
Принадлежит: Conagen Inc.

Disclosed are steviol glycosides referred to as rebaudioside V and rebaudioside W. Also disclosed are methods for producing rebaudioside M (Reb M), rebausoside G (Reb G), rebaudioside KA (Reb KA), rebaudioside V (Reb V) and rebaudioside (Reb W). 132-. (canceled)33. A method of producing of rebaudioside M from stevioside , the method comprising:preparing a reaction mixture comprising stevioside; substrates selected from the group consisting of sucrose, uridine diphosphate (UDP), uridine diphosphate-glucose (UDP-glucose), and combinations thereof; and a uridine dipospho glycosyltransferase (UDP-glycosyltransferase) selected from the group consisting of an UGT76G1, an HV1, a UDP-glycosyltransferase fusion enzyme, and combinations thereof; andincubating the reaction mixture for a sufficient time to produce rebaudioside M, wherein a glucose is covalently coupled to the stevioside by UGT76G1 or the fusion enzyme to produce rebaudioside A, a glucose is covalently coupled to the rebaudioside A by HV1 to produce rebaudioside D, and a glucose is covalently coupled to the rebaudioside D by UGT76G1 or the fusion enzyme to produce rebaudioside M.34. The method of further comprising adding a sucrose synthase to the reaction mixture.35ArabidopsisArabidopsisVigna radiate. The method of claim 34 , wherein the sucrose synthase is selected from the group consisting of an sucrose synthase 1; an sucrose synthase 3; and a sucrose synthase.36Arabidopsis thaliana. The method of claim 35 , wherein the sucrose synthase is an sucrose synthase 1.37. The method of claim 33 , wherein the UDP-glycosyltransferase fusion enzyme is an UGT76G1 uridine diphospho glycosyltransferase domain coupled to a sucrose synthase domain.38ArabidopsisArabidopsisVigna radiate. The method of claim 37 , wherein the sucrose synthase domain is selected from the group consisting of an sucrose synthase 1; an sucrose synthase 3; and a sucrose synthase.39Arabidopsis thaliana. The method of claim 38 , wherein the sucrose ...

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Номер записи: 48
01-03-2018 дата публикации

NON-CALORIC SWEETENERS AND METHODS FOR SYNTHESIZING

Номер: US20180057522A1
Автор: Mao Guohong, Yu Xiaodan
Принадлежит: Conagen Inc.

Disclosed are steviol glycosides referred to as rebaudioside V and rebaudioside W. Also disclosed are methods for producing rebaudioside M (Reb M), rebausoside G (Reb G), rebaudioside KA (Reb KA), rebaudioside V (Reb V) and rebaudioside (Reb W). 139-. (canceled)40. A method of producing of rebaudioside M from rebaudioside A , the method comprising:preparing a reaction mixture comprising rebaudioside A; substrates selected from the group consisting of sucrose, uridine diphosphate (UDP), uridine diphosphate-glucose (UDP-glucose), and combinations thereof; and a uridine dipospho glycosyltransferase (UDP-glycosyltransferase) selected from the group consisting of an UGT76G1, an HV1, a UDP-glycosyltransferase fusion enzyme, and combinations thereof, andincubating the reaction mixture for a sufficient time to produce rebaudioside M, wherein a glucose is covalently coupled to the rebaudioside A by HV1 to produce rebaudioside D, and a glucose is covalently coupled to the rebaudioside D by UGT76G1 or the fusion enzyme to produce rebaudioside M.41. The method of further comprising adding a sucrose synthase to the reaction mixture.42ArabidopsisArabidopsisVigna radiate. The method of claim 41 , wherein the sucrose synthase is selected from the group consisting of an sucrose synthase 1; an sucrose synthase 3; and a sucrose synthase.43Arabidopsis thaliana. The method of claim 42 , wherein the sucrose synthase is an sucrose synthase 1.44. The method of claim 40 , wherein the UDP-glycosyltransferase fusion enzyme is an UGT76G1 uridine diphospho glycosyltransferase domain coupled to a sucrose synthase domain.45ArabidopsisArabidopsisVigna radiate. The method of claim 44 , wherein the sucrose synthase domain is selected from the group consisting of an sucrose synthase 1; an sucrose synthase 3; and a sucrose synthase.46Arabidopsis thaliana. The method of claim 45 , wherein the sucrose synthase domain is an sucrose synthase 1.47. The method of claim 40 , wherein the reaction mixture ...

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Номер записи: 49
01-03-2018 дата публикации

UDP-GLYCOSYLTRANSFERASES FROM SOLANUM LYCOPERSICUM

Номер: US20180057850A1
Автор: BEEKWILDER Martinus Julius, BOER Viktor Marius, Bosch Hendrik Jan
Принадлежит:

The present invention relates to polypeptides having UDP-Glycosyltransferase activity derived from Solanum lycopersicum and having the amino acid sequence set out in any of SEQ ID NO: 1 to 4 or an amino acid sequence having at least about 30% sequence identity thereto. The application also relates to recombinant hosts comprising a recombinant nucleic acid sequence encoding said polypeptides and uses thereof prepare glycosylated diterpenes, like steviol glycoside. The host cells might comprise further enzymes of the steviol glycoside biosynthesis pathway. 1. A recombinant host comprising a recombinant nucleic acid sequence encoding a polypeptide comprising:a. the amino acid sequence set forth in SEQ ID NO: 1 or an amino acid sequence having at least about 30% sequence identity thereto;b. the amino acid sequence set forth in SEQ ID NO: 2 or an amino acid sequence having at least about 30% sequence identity thereto;c. the amino acid sequence set forth in SEQ ID NO: 3 or an amino acid sequence having at least about 30% sequence identity thereto; ord. the amino acid sequence set forth in SEQ ID NO: 4 or an amino acid sequence having at least about 30% sequence identity thereto.2. A recombinant host according to which is capable of producing a glycosylated diterpene.3. A recombinant host according to which comprises one or more recombinant nucleotide sequence(s) encoding:a polypeptide having ent-copalyl pyrophosphate synthase activity;a polypeptide having ent-Kaurene synthase activity;a polypeptide having ent-Kaurene oxidase activity; and/ora polypeptide having kaurenoic acid 13-hydroxylase activity.4. A recombinant host according to claim 1 , which comprises a recombinant nucleic acid sequence encoding a polypeptide having NADPH-cytochrome p450 reductase activity.5. A recombinant host according to which comprises a recombinant nucleic acid sequence encoding one or more of:(i) a polypeptide having UGT74G1 activity (UGT3 activity);(ii) a polypeptide having UGT85C2 activity ...

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Номер записи: 50
17-03-2022 дата публикации

HEAT TREATMENT TO PRODUCE GLYCOSIDES

Номер: US20220081699A1
Автор: Anderson James C., BROWER, Carlson Ting Liu, FLORES Belit, Gaspard Dan S., III Robert J., MORTENSON Kristopher T., NYGAARD Richard, PAULSON Nicole, Rasmussen Maribeth
Принадлежит:

Disclosed are methods of releasing or enriching for steviol glycosides produced by yeast. The disclosed methods enhance or improve the release or enrichment of the steviol glycosides. 1. A method of releasing steviol glycosides from a yeast host cell , comprising the steps of:(a) providing a composition, comprising the host cell producing one or more steviol glycosides; and(b) releasing the one or more steviol glycosides from the host cell by heating the composition to a temperature in a range from 50° C. to 95° C. for 5 minutes to 48 hours, wherein the one or more steviol glycosides are released from an intracellular portion of the host cell into an extracellular portion of the composition.2. The method of claim 1 , wherein the composition is a liquid composition comprising the host cell and a liquid media.3. The method of claim 2 , wherein the composition is obtained by separating the host cell from a fermentation medium comprising the host cell claim 2 , and transferring the separated host cell into the suitable liquid media.4. The method of claim 1 , wherein the composition is a fermentation medium.5. The method of claim 1 , wherein the host cell is concentrated before the heat treatment.6. The method of claim 1 , wherein the composition is heated to the temperature in the range from 70° C. to 95° C. for 5 minutes to 2 hours.7. The method of claim 1 , wherein the composition is heated to about 50° C. for 24 hours.8. The method of claim 1 , wherein the composition is heated to about 70° C. for 5 minutes to 2 hours.9. The method of claim 1 , wherein the composition is heated to about 75° C. for 5 minutes to 2 hours.10. The method of claim 1 , wherein the composition is heated to about 95° C. for 5 minutes to 1 hour.11. The method of claim 1 , wherein an amount of the steviol glycosides released extracellularly is in a range of about 80% to about 100% of a total intracellular and extracellular amount of the steviol glycosides produced by the host cell.12. The ...

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Номер записи: 51
28-02-2019 дата публикации

PRODUCTION OF STEVIOL GLYCOSIDES IN RECOMBINANT HOSTS

Номер: US20190062796A1
Автор: Douchin Veronique, Dyekjaer Jane Dannow, Houghton-Larsen Jens
Принадлежит:

The invention relates to recombinant microorganisms and methods for producing steviol glycosides and steviol glycoside precursors. 1. A recombinant host cell , comprising a recombinant gene encoding a polypeptide capable of glycosylating a steviol or a steviol glycoside at its C-19 carboxyl group and having at least 50% sequence identity to the amino acid sequence set forth in SEQ ID NOs:2 or 119 , wherein the recombinant host cell is capable of producing the steviol glycoside or a steviol glycoside composition.2. The recombinant host cell of claim 1 , further comprising:(a) a gene encoding a polypeptide capable of synthesizing geranylgeranyl pyrophosphate (GGPP) from farnesyl diphosphate (FPP) and isopentenyl diphosphate (IPP);(b) a gene encoding a polypeptide capable of synthesizing ent-copalyl diphosphate from GGPP;(c) a gene encoding an a polypeptide capable of synthesizing ent-kaurene from ent-copalyl pyrophosphate;(d) a gene encoding a polypeptide capable of synthesizing ent-kaurenoic acid from ent-kaurene;(e) a gene encoding a polypeptide capable of reducing cytochrome P450 complex;(f) a gene encoding a polypeptide capable of synthesizing steviol from ent-kaurenoic acid;(g) a gene encoding a polypeptide capable of glycosylating the steviol or the steviol glycoside at its C-13 hydroxyl group;(h) a gene encoding a polypeptide capable of beta 1,3 glycosylation of the C3′ of the 13-O-glucose, 19-O-glucose, or both 13-O-glucose and 19-O-glucose of a steviol glycoside;(i) a gene encoding a second polypeptide capable of glycosylating the steviol or the steviol glycoside at its C-19 carboxyl group;(j) a gene encoding a polypeptide capable of beta 1,2 glycosylation of the C2′ of the 13-O-glucose, 19-O-glucose, or both 13-O-glucose and 19-O-glucose of a steviol glycoside; and/or(k) a gene encoding a bifunctional polypeptide capable of synthesizing ent-copalyl diphosphate from GGPP and synthesizing ent-kaurene from ent-copalyl pyrophosphate.wherein at least one of the ...

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Номер записи: 52
11-03-2021 дата публикации

STEVIOL GLYCOSIDE SOLUTIONS

Номер: US20210068437A1
Автор: JOHNSON Marquita L., Kim Grace, MARKOSYAN Avetik, Martin John, TAN KIAN PIN
Принадлежит:

Steviol glycoside solutions, and methods of making steviol glycoside solutions, are described. The steviol glycoside solutions are clear and stable, and have clear solution stability, for extended periods of time. 1. A method of making a stable , clear liquid steviol glycoside ingredient solution , comprising the steps of:a. providing a steviol glycoside;b. providing a solvent system comprising 10-100% w/w propylene glycol; andc. combining the steviol glycoside in the solvent with constant agitation and while maintaining the temperature of the solvent until the steviol glycoside is dissolved;wherein the stable, clear liquid steviol glycoside ingredient solution has a clear solution stability at a steviol glycoside concentration of up to 40% w/w for 45-60 days.2. The method of claim 1 , wherein the steviol glycoside is made by a gradient heating and gradient cooling process.3. The method of claim 1 , wherein the propylene glycol is 10-50% w/w.4. The method of claim 1 , wherein the solvent system is heated prior to combining the steviol glycosides in the solvent.5. The method of claim 4 , wherein the solvent system is heated to a temperature of about 70° C. to about 95° C.6. The method of claim 1 , wherein the steviol glycoside ingredient solution is cooled after dissolving the steviol glycoside.7. The method of claim 6 , wherein the steviol glycoside ingredient solution is cooled to about 20° C.8. The method of claim 1 , wherein the steviol glycoside ingredient solution is visibly clear and free of visible cloudiness or turbidity.9. The method of claim 1 , wherein the steviol glycoside is selected from one or more of the following: Reb A claim 1 , Reb B claim 1 , Reb C claim 1 , Reb D claim 1 , Reb E claim 1 , Reb F claim 1 , Reb I claim 1 , Reb M claim 1 , Reb N claim 1 , Reb O claim 1 , Reb Q claim 1 , Reb R claim 1 , Dulcoside A claim 1 , Rubusoside claim 1 , Stevioside claim 1 , Steviolbioside claim 1 , and combinations thereof. The invention is directed to a ...

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Номер записи: 53
15-03-2018 дата публикации

STEVIOL GLYCOSIDES

Номер: US20180070622A1
Автор: GOSIEWSKA Silvia, Lankhorst Peter Philip, VAN DER HOEVEN Robertus Antonius Mijndert
Принадлежит:

The present invention relates to a steviol glycoside having the formula of (I) 8. A steviol glycoside according to which is fermentatively produced.11. A method for production of a steviol glycoside according to claim 1 , which method comprises:providing a recombinant yeast cell comprising recombinant nucleic acid sequences encoding polypeptides comprising the amino acid sequences encoded by: SEQ ID NO: 61, SEQ ID NO: 65, SEQ ID NO: 23, SEQ ID NO: 33, SEQ ID NO: 77, SEQ ID NO: 71, SEQ ID NO: 87, SEQ ID NO: 73 and SEQ ID NO: 75;fermenting the recombinant yeast cell in a suitable fermentation medium; and, optionally,recovering a steviol glycoside according to any one of the preceding claims.12. A composition comprising a steviol glycoside according to and one or more different steviol glycosides.13. A foodstuff claim 1 , feed and/or beverage which comprises a steviol glycoside according to or a composition thereof.14. A product comprising a steviol glycoside according to or a composition in a sweetener composition or flavor composition.15. A product comprising a steviol glycoside according to or a composition thereof in a foodstuff claim 1 , feed and/or beverage. The present invention relates to steviol glycosides, to methods for producing them, to sweetener compositions, flavour compositions, foodstuffs, feeds and beverages comprising the steviol glycosides and to use of the steviol glycosides in sweetener compositions, flavour compositions, foodstuffs, feeds and beverages.The leaves of the perennial herb, Bert., accumulate quantities of intensely sweet compounds known as steviol glycosides. Whilst the biological function of these compounds is unclear, they have commercial significance as alternative high potency sweeteners.These sweet steviol glycosides have functional and sensory properties that appear to be superior to those of many high potency sweeteners. In addition, studies suggest that stevioside can reduce blood glucose levels in Type II diabetics and can ...

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Номер записи: 54
15-03-2018 дата публикации

Method of converting RB to RD by using cutinase under stepwise changing temperatures

Номер: US20180073049A1
Автор: FANG Yun, WANG Haijun, XIA Yongmei
Принадлежит: JIANGNAN UNIVERSITY

The present invention provides a method to produce RD by using a cutinase to catalyze the esterification of RB under stepwise cooling temperatures, which is related to the field of biosynthesis of organic compounds. The method uses a cutinase from to catalyze the esterification of RB and sophorose to produce RD. The stepwise cooling temperatures are used to reduce the heat inactivation of the enzyme as well as to improve the mass transfer. The method catalyzes the esterification of RB to produce RD in a solvent such as methanol, DMSO and DMF. The reaction is safe, efficient and highly selective. In addition, the method uses stepwise additions of substrate RB and cooling temperatures for the esterification reaction. In this way, it speeds up the initial reaction rate, increases the amount of solved RB as it is converted to RD, and improves the mass transfer to further increase the reaction speed. In summary, the method uses moderate reaction conditions, and has a high yield and a simple purification procedure. 1. A method for producing Rebaudioside D (RD) from Rebaudioside B (RB) , wherein said method uses a cutinase to catalyze the esterification of RB and sophorose to produce RD under stepwise cooling temperatures.3. The method of claim 1 , comprises the following steps:(1) dissolving substrate RB in a solvent to 10-20 g/L;(2) adding the cutinase to the substrate at 55° C. to obtain a reaction mixture;(3) mixing well the reaction mixture obtained from step (2) and heating it up to 70° C. to react for 0.5-1 hour;(4) replenishing the reaction mixture with 10-20 g/L RB and continuing the reaction at 60° C. for 1-1.5 hours; and(5) replenishing the reaction mixture again with 30-50 g/L RB and continuing the reaction at 60° C. for 2-6 hours.4. The method of claim 1 , wherein the added cutinase can be an enzyme solution claim 1 , powdered enzyme claim 1 , or immobilized enzyme.5. The method of claim 4 , wherein the cutinase can be obtained by physical claim 4 , chemical ...

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Номер записи: 55
15-03-2018 дата публикации

EXTRACELLULAR DITERPENE PRODUCTION

Номер: US20180073050A1
Автор: BOER Viktor Marius, BROERS Nicolette Jasmijn, LAWRENCE Adam G.
Принадлежит:

The present invention relates to a method for the production of a diterpene or a glycosylated diterpene, which method comprises: a. fermenting a recombinant microorganism in a suitable fermentation medium, wherein the microorganism comprises one or more nucleotide sequence(s) encoding: a polypeptide having ent-copalyl pyrophosphate synthase activity; a polypeptide having ent-Kaurene synthase activity; a polypeptide having ent-Kaurene oxidase activity; and a polypeptide having kaurenoic acid 13-hydroxylase activity and whereby expression of the nucleotide sequence(s) confer(s) on the microorganism the ability to produce at least steviol, whereby a diterpene or glycosylated diterpene is produced extracellularly in the fermentation medium; and b. recovering the diterpene or glycosylated diterpene from the fermentation medium. 1. A method for the production of a diterpene or a glycosylated diterpene , which method comprises:a. fermenting a recombinant microorganism in a suitable fermentation medium,wherein the microorganism comprises one or more nucleotide sequence(s) encoding: a polypeptide having ent-copalyl pyrophosphate synthase activity; a polypeptide having ent-Kaurene synthase activity; a polypeptide having ent-Kaurene oxidase activity; and a polypeptide having kaurenoic acid 13-hydroxylase activity and whereby expression of the nucleotide sequence(s) confer(s) on the microorganism the ability to produce at least steviol,whereby a diterpene or glycosylated diterpene is produced extracellularly in the fermentation medium; andb. recovering the diterpene or glycosylated diterpene from the fermentation medium.2. The method according to claim 1 , wherein the recombinant microorganism comprises one or more nucleotide sequences encoding a polypeptide having UDP-glucosyltransferase activity claim 1 ,whereby expression of the nucleotide sequence(s) confer(s) on the microorganism the ability to produce at least one of steviolmonoside, steviolbioside, stevioside or ...

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Номер записи: 56
24-03-2022 дата публикации

URIDINE DIPHOSPHATE-DEPENDENT GLYCOSYLTRANSFERASE ENZYME

Номер: US20220090031A1
Автор: DONALD Jason Eric, KUMARAN Ajikumar Parayil, LOVE Aaron, SANTOS Christine Nicole S., TOOMEY Christopher
Принадлежит:

In various aspects, the present invention provides uridine diphosphate-dependent glycosyltransferase (UGT) enzymes capable of catalyzing the transfer of a monosaccharide moiety from a NDP-sugar to the 3′ carbon of a sugar moiety of a substrate, such as a terpenoid glycan, thereby functioning as a “1-3 UGT.” In other aspects, the invention provides polynucleotides encoding the 1-3 UGT, and host cells comprising the same. In still other aspects, the invention provides methods for preparing glycosylated substrates, including steviol glycosides, using the enzyme and host cells of this disclosure. 1. A uridine diphosphate-dependent glycosyltransferase (UGT) enzyme comprising an amino acid sequence that is at least about 75% identical to the amino acid sequence of SEQ ID NO: 5 or SEQ ID NO: 6.210-. (canceled)11. The enzyme of claim 1 , wherein the enzyme comprises an amino acid substitution at positions corresponding to one or more of positions 29 claim 1 , 200 claim 1 , 357 claim 1 , and 414 of SEQ ID NO: 1.12. The enzyme of claim 1 , comprising one or more of:a glycine (G) at the position corresponding to position 54 of SEQ ID NO: 5;a leucine (L) at the position corresponding to position 111 of SEQ ID NO: 5; anda methionine (M) at the position corresponding to position 183 of SEQ ID NO: 5.13. The enzyme of claim 12 , wherein the enzyme has Gly at the position corresponding to position 54 of SEQ ID NO: 5 claim 12 , Leu at the position corresponding to position 111 of the SEQ ID NO: 5 claim 12 , and a Met at the position corresponding to position 183 of SEQ ID NO: 5.14. The enzyme of claim 1 , wherein the enzyme comprises an insertion of from about 6 to about 12 amino acids after the position corresponding to position 155 of SEQ ID NO: 5.15. The enzyme of claim 14 , wherein the insertion is a flexible and hydrophilic sequence that is predominately Glycine and Serine residues claim 14 , and is optionally GSGGSG (SEQ ID NO: 7) or GSGGSGGSG (SEQ ID NO: 8).16. The enzyme of ...

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Номер записи: 57
24-03-2022 дата публикации

GERANYLGERANYL PYROPHOSPHATE SYNTHASES

Номер: US20220090032A1
Автор: BOER Viktor Marius, VAN LEEUWEN Johannes Gustaaf Ernst, ZWARTJENS Priscilla
Принадлежит:

The present invention relates a variant polypeptide having geranylgeranyl pyrophosphate synthase activity, which variant polypeptide comprises an amino acid sequence which, when aligned with a geranylgeranyl pyrophosphate synthase comprising the sequence set out in SEQ ID NO: 1, comprises at least one substitution of an amino acid residue corresponding to any of amino acids at positions 92, 100 or 235 said positions being defined with reference to SEQ ID NO: 1 and wherein the variant has one or more modified properties as compared with a reference polypeptide having geranylgeranyl pyrophosphate synthase activity. A variant polypeptide of the invention may be used in a recombinant host for the production of steviol or a steviol glycoside. 1. A process for preparing steviol or a steviol glycoside , said process comprising fermenting a recombinant host in a suitable fermentation medium , wherein the recombinant host comprises a polynucleotide encoding a variant polypeptide having geranylgeranyl pyrophosphate synthase activity , which variant polypeptide comprises an amino acid sequence which , when aligned with a geranylgeranyl pyrophosphate synthase comprising the sequence of SEQ ID NO:1 , comprises a substitution of an amino acid residue corresponding to any of amino acids at positions92, 100, or 235,said positions being relative to SEQ ID NO:1.2. The process of claim 1 , wherein the variant polypeptide has modified geranylgeranyl pyrophosphate synthase activity as compared with a reference polypeptide having geranylgeranyl pyrophosphate synthase activity claim 1 , and wherein the reference polypeptide comprises the geranylgeranyl pyrophosphate synthase of SEQ ID NO:1 or SEQ ID NO:17.3. The process of claim 1 , wherein the variant polypeptide comprises a substitution relative to SEQ ID NO:1 selected from the group consisting of G92E claim 1 , G92D claim 1 , G92N claim 1 , and G92Q.4. The process of claim 1 , wherein the variant polypeptide comprises a substitution ...

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Номер записи: 58
18-03-2021 дата публикации

COMPOSITION COMPRISING GLUCOSYLATED TERPENE GLYCOSIDES, TERPENE GLYCOSIDES AND CYCLODEXTRINE

Номер: US20210076719A1
Автор: Haefliger Olivier
Принадлежит: FIRMENICH SA

The various aspects presented herein relate to processes for preparing food ingredients, flavors and sweeteners from extracts of plants that contain terpene glycosides such as Stevia rebaudiana Bertoni, Rubus suavissimus or Siraitia grosvenorii. Also presented herein are formulations and uses of compositions made from the processes. 1. A composition ,wherein the composition is a flavor and taste modifying composition, a) glucosylated terpene glycosides of different degrees of glucosylation;', 'b) residual terpene glycoside molecules selected from the group consisting of stevioside, rebaudioside A, rebaudioside C, rebaudioside D, other steviol glycosides, rubusoside, mogrosides, and any combination thereof; in an amount ranging from 1% to 40% on a dry basis; and', 'c) at least one cyclodextrin., 'wherein the composition comprises2. The composition of claim 1 , wherein the glucosylated terpene glycosides comprise glucosylated steviol glycosides.3. The composition of claim 1 , wherein the glucosylated terpene glycosides comprise glucosylated rubusoside.4. The composition of claim 1 , wherein the glucosylated terpene glycosides comprise glucosylated mogrosides.5. The composition of claim 1 , wherein the glucosylated mogrosides comprise glucosylated mogroside V.6. The composition of claim 1 , wherein the amount of the at least one cyclodextrin in the composition is from 0.001 to 14% w/w.7. The composition of claim 1 , wherein the amount of the at least one cyclodextrin in the composition is from 0.01 to 14% w/w.8. The composition of claim 1 , wherein the different degrees of glucosylation comprises from 1 to 20 glucose residues attached to the terpene glycoside via a glucosidic bond.9. The composition of claim 1 , wherein the residual terpene glycoside molecules comprise steviol glycoside molecules.10. The composition of claim 1 , wherein the residual terpene glycoside molecules comprise rebaudioside A molecules.11. The composition of claim 1 , wherein the residual ...

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Номер записи: 59
18-03-2021 дата публикации

Method to Elevate the Amount of Steviol Glycosides Remained in the Recrystallization Mother Liquor of Stevia Extract

Номер: US20210079437A1
Автор: Fan Ye, FANG Yun, HU Xueyi, LIU Xiang, WANG Haijun, Wu Jing, XIA Yongmei, Xu Fei, ZHOU Zhuoyu
Принадлежит:

The disclosure discloses a method to elevate the amount of steviol glycosides remained in the recrystallization mother liquor of extract, and belongs to the field of modification and extraction of natural compounds. The disclosure adopts lactase for highly specific catalytic hydrolysis of stevioside to convert stevioside into rubusoside which usually can only be obtained from leaves of S. Lee; and meanwhile, after stevioside in the mother liquor glycosides is converted, since the polarity difference between rubusoside and rebaudioside A is far larger than the polarity difference between stevioside and rebaudioside A, rebaudioside A is more easily separated out of the mother liquor glycosides. A technology provided by the disclosure is also applicable to value increment of mixed steviol glycosides containing stevioside and is beneficial for separating out rebaudioside A from the mixed steviol glycosides. 1Stevia. A method of elevating amount of steviol glycosides remained in recrystallization mother liquor of extract for preparing rubusoside , comprising:{'i': Stevia', 'Kluyveromyces fragilis', 'Stevia', 'Stevia, 'with mother liquor glycosides as a substrate, using lactase from to assist a reaction for 1-3 h at a temperature of 25-45° C. for specific catalytic hydrolysis of stevioside in the mother liquor glycosides, so that stevioside is hydrolyzed into rubusoside, wherein rebaudioside A does not participate in the reaction but continues to stay in the mother liquor glycosides; and'}after the reaction ends, separating out rubusoside and rebaudioside A;{'i': 'Stevia', 'wherein an enzyme concentration of the lactase is 10-100 U/g stevioside, and mass of the added lactase does not exceed 1% of mass of the mother liquor glycosides used.'}2SteviaStevia rebaudiana. The method according to claim 1 , wherein the mother liquor glycosides are mother liquor or a dry product of the mother liquor generated after the steviol glycosides are extracted or purified in a process of ...

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Номер записи: 60
22-03-2018 дата публикации

STEVIOL GLYCOSIDE

Номер: US20180077959A1
Автор: FUJITA Isao, MATSUURA Fumito, MORITA Toyoshige, OTA Masaya
Принадлежит:

Nobel Sweetening components are provided. Through the analysis of the components of the nobel Steviol Glycoside included in the extract and/or crystals, not only the quality control of sweeteners, but judgment on the correctness of indication of origin or infringement of right are facilitated since the raw material of the sweetener can be identified. 17.-. (canceled)9Stevia Rebaudiana. The method of claim 8 , wherein the Steviol glycoside VIII is purified from Bertoni.10. The method of claim 8 , further comprising crystallizing an extract obtained from the purifying step.11. The method of claim 8 , wherein the purifying comprises use of a column.12. The method of claim 11 , further comprising crystallizing an extract obtained from the purifying step.13. The method of claim 12 , wherein the crystals comprise a larger amount of the Steviol glycoside VIII than Rebaudioside A.14. The method of claim 12 , wherein the crystals comprise a larger amount of the Steviol glycoside VIII than any other Steviol glycoside.15. The method of claim 8 , wherein the purifying comprises adsorbing the Steviol glycoside VIII to an adsorbent resin and eluting the Steviol glycoside VIII from the adsorbent resin to produce an extract.16. The method of claim 15 , further comprising crystallizing the extract obtained from the purifying step.17. The method of claim 13 , wherein the extract comprises a larger amount of the Steviol glycoside VIII than any other Steviol glycoside.18. The method of claim 8 , wherein the sweetener comprises a larger amount of the Steviol glycoside VIII than any other Steviol glycoside.19. The method of claim 8 , wherein an extract obtained from the purifying step comprises a larger amount of the Steviol glycoside VIII than Rebaudioside A.20. A method of preparing a food product claim 8 , the method comprising{'claim-ref': {'@idref': 'CLM-00008', 'claim 8'}, 'manufacturing the sweetener according to the method of , and'}mixing the sweetener and a food to form the ...

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Номер записи: 61
23-03-2017 дата публикации

IMPROVED SWEETENER

Номер: US20170079313A1
Автор: BRIDGES John R., COHEN Jason C., Fletcher Joshua Nehemiah, WOODYER Ryan D., ZHOU Yuquing
Принадлежит: Tate & Lyle Ingredients Americas LLC

A low or zero calorie sweetener composition with sweetness synergy and desirable taste characteristics. The sweetener composition is suitable for use as a substitute for high calorie sugars. The sweetener composition is for use in food and beverage products, pharmaceutical products, nutritional product and cosmetic products. 1stevia. A sweetener composition comprising allulose and a extract.2stevia. The sweetener composition according to claim 1 , wherein the extract comprises at least one steviol glycoside.3. The sweetener composition according to claim 2 , wherein the at least one steviol glycoside is selected from the group consisting of Rebaudioside A claim 2 , Rebaudioside B claim 2 , Rebaudioside C claim 2 , Rebaudioside D claim 2 , Rebaudioside E claim 2 , Rebaudioside F claim 2 , Rebaudioside M claim 2 , Rebaudioside X claim 2 , Rubusoside claim 2 , Stevioside and Dulcosides claim 2 , and mixtures thereof.4. The sweetener composition according to claim 2 , wherein the at least one steviol glycoside comprises Rebaudioside A.5. The sweetener composition according to claim 2 , wherein the at least one steviol glycoside comprises Rebaudioside B.6. The sweetener composition according to claim 2 , wherein the at least one steviol glycoside comprises Rebaudioside A and Rebaudioside B.7steviastevia. The sweetener composition according to claim 1 , wherein the extract comprises steviol glycosides in a total amount of at least 90 weight % claim 1 , relative to the total weight of the extract on a dry solids basis.8steviastevia. The sweetener composition according to claim 1 , wherein the extract comprises Rebaudioside A and Stevioside in a combined total amount of at least 70 weight % claim 1 , relative to the total weight of the extract on a dry solids basis.9steviastevia. The sweetener composition according to claim 1 , wherein the extract comprises Rebaudioside A in an amount of from about 60 weight % to about 85 weight % claim 1 , relative to the combined total ...

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Номер записи: 62
23-03-2017 дата публикации

HIGHLY SOLUBLE STEVIOL GLYCOSIDES

Номер: US20170079317A1
Автор: Carlson Ting Liu, Goulson Melanie Jean, Knutson Nathan Edward
Принадлежит: Cargill, Incorporated

The present invention provides a liquid composition comprising one or more steviol glycosides, wherein the one or more steviol glycosides comprise Rebaudioside O. The present invention also provides a method of making a liquid composition comprising one or more steviol glycosides, wherein the one or more steviol glycosides comprise Rebaudioside O. The liquid compositions are useful for applications such as beverages, carbonated beverages, concentrates, beverage concentrates, throw syrups, or portable liquid concentrate tabletop applications. 1. A liquid composition comprising one or more steviol glycosides , wherein:a. the concentration of the one or more steviol glycosides in the composition comprises at least 0.1 wt % of the composition,b. the one or more steviol glycosides comprise Rebaudioside O; andc. the liquid composition is an aqueous composition.2. The composition of claim 1 , wherein the concentration of the one or more steviol glycosides in the composition comprises at least 1-10 wt % of the composition.3. The composition of claim 1 , wherein the concentration of the one or more steviol glycosides is at least 5 wt % of the composition.4. The composition of claim 1 , wherein the concentration of the one or more steviol glycosides comprises 2-6 wt % of the composition.5. The composition of claim 1 , wherein the one or more steviol glycosides comprises 1-95 wt % Rebaudioside O.6. The composition of claim 1 , wherein the one or more steviol glycosides comprises 15-50 wt % Rebaudioside O.7. The composition of claim 1 , wherein the pH of the composition is less than 5.8. The composition of claim 1 , wherein the pH of the composition ranges from 2 to 5.9. The composition of claim 1 , wherein the composition comprises a concentrate.10. The composition of claim 1 , wherein the composition comprises a throw syrup.11. A method of making a liquid concentrate comprising the steps of providing one or more steviol glycosides wherein the one or more steviol glycosides ...

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Номер записи: 63
31-03-2022 дата публикации

SWEETENER COMPOSITION AND METHOD FOR PREPARING SAME

Номер: US20220095656A1
Автор: CHOI Eun Jung, Chu Sun, KIM Jung Eun, Kim Seong Bo, Park Sung Hee
Принадлежит: CJ CHEILJEDANG CORPORATION

The present invention relates to a sweetening material composition and a method for preparing the same. Specifically, the present invention relates to a sweetening material composition including transglycosylation steviol glycosides and a method for preparing the same. The sweetening material composition of the present invention includes transglycosylated and saccharides, and the saccharides include 5 to 90 parts by weight of an oligosaccharide having a degree of polymerization (DP) of 3 or more with respect to 100 parts by weight. 1. A sweetening material composition comprising:{'i': 'stevia', 'transglycosylated and saccharides,'}wherein the saccharides comprise 5 to 90 parts by weight of an oligosaccharide having a degree of polymerization (DP) of 3 or more with respect to 100 parts by weight.2. The sweetening material composition of claim 1 , wherein the saccharides are contained in an amount of 10 to 60% by weight with respect to the total weight of the sweetening material composition.3stevia. The sweetening material composition of claim 1 , wherein the transglycosylated comprises at least one glucose added to the OH linked at position of carbon 19 in steviol glycosides.4Lactobacillus mali. A composition for preparing a sweetening material comprising disaccharides containing glucose claim 1 , steviol glycosides claim 1 , and or a glycosyltransferases derived therefrom.5. The composition for preparing a sweetening material of claim 4 , wherein the disaccharides containing glucose comprise at least one of sucrose and maltose.6. The composition for preparing a sweetening material of claim 5 , wherein the disaccharides containing glucose comprise sucrose and maltose in a mixed weight ratio of 1:20 to 20:1.7. A method for preparing a sweetening material composition claim 5 , the method comprising:{'i': mali', 'stevia, 'contacting a mixture of disaccharides containing glucose and steviol glycosides with Lactobacilli or a glycosyltransferase derived therefrom to ...

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Номер записи: 64
14-03-2019 дата публикации

Biosynthetic production of steviol glycosides and processes therefore

Номер: US20190078102A1
Автор: Beihua ZHANG, David BYUN, Guohong Mao, Jacob Edward Vick, Michael Batten, Xiaodan Yu, Yang Luo, Yilin Wu
Принадлежит: Conagen Inc

The present invention relates to the production of steviol glycoside rebaudiosides D4, WB1 and WB2 and the production of rebaudioside M from Reb D4.

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Номер записи: 65
14-03-2019 дата публикации

Production of Steviol Glycosides in Microorganisms

Номер: US20190078128A9
Автор: Blom Charlotte, Hansen Esben Halkjaer, Hansen Jorgen, HICKS Paula M., Houghton-Larsen Jens, Kishore Ganesh M., Mikkelsen Michael Dalgaard, Motion Michael, Tavares Sabina
Принадлежит:

Recombinant microorganisms, plants, and plant cells are disclosed that have been engineered to express novel recombinant genes encoding steviol biosynthetic enzymes and UDP-glycosyltransferases (UGTs). Such microorganisms, plants, or plant cells can produce steviol or steviol glycosides, e.g., rubusoside or Rebaudioside A, which can be used as natural sweeteners in food products and dietary supplements. 1. A recombinant host cell capable of producing steviol , a target steviol glycoside or a target steviol glycoside composition , comprising: 'wherein the polypeptide is capable of transferring a sugar moiety to the C2′ of a glucose in the precursor steviol glycoside;', '(a) a gene encoding a polypeptide capable of beta 1,2 glycosylation of the C2′ of the 13-O-glucose, 19-O-glucose, or both 13-O-glucose and 19-O-glucose of a precursor steviol glycoside;'}and one or more of:(b) a gene encoding a polypeptide capable of glycosylating steviol or the precursor steviol glycoside at its C-13 hydroxyl group; and/or(c) a gene encoding a polypeptide capable of beta 1,3 glycosylation of the C3′ of the 13-O-glucose of the precursor steviol glycoside; and/or(d) a gene encoding a polypeptide capable of glycosylating steviol or the precursor steviol glycoside at its C-19 carboxyl group;wherein at least one of the genes is a recombinant gene.2. The recombinant host cell of claim 1 , wherein:(a) the precursor steviol glycoside is rubusoside, wherein the sugar moiety is glucose, and stevioside is produced upon transfer of the glucose moiety;(b) the precursor steviol glycoside is stevioside, the sugar moiety is glucose, and rebaudioside E is produced upon transfer of the glucose moiety;(c) the precursor steviol glycoside is stevioside, the sugar moiety is glucose, the stevioside is contacted with the polypeptide capable of beta 1,2 glycosylation of the C2′ of the 13-O-glucose, 19-O-glucose, or both 13-O-glucose and 19-O-glucose of the precursor steviol glycoside and a polypeptide ...

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Номер записи: 66
22-03-2018 дата публикации

Production of non-caloric sweeteners using engineered whole-cell catalysts

Номер: US20180080055A1
Автор: Guohong Mao, Jacob Edward Vick, Shi-Yan Li, Xiaodan Yu
Принадлежит: Conagen Inc

Disclosed are whole-cell catalysts, methods of making the whole-cell catalysts, and methods of using the whole-cell catalysts to produce steviol glycosides.

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Номер записи: 67
12-03-2020 дата публикации

COMPOSITIONS OF STEVIOL MULTIGLYCOSYLATED DERIVATIVES AND STEVIA COMPONENTS

Номер: US20200077687A1
Автор: Jackson Mel Clinton
Принадлежит:

The invention describes a sweetener of glycosylated steviol glycosides and a rebaudioside in an aqueous solution for convenience of use in the food and beverage industry. 129-. (canceled)30. A composition comprising:{'i': 'Stevia', 'a first component comprising at least 70% by weight glycosylated steviol glycosides (GSGs), wherein the first component is derived from a extract comprising 20-99.5% rebaudioside A (RA) by weight; and'}a second component comprising 20-99.5% RA by weight,wherein the weight ratio of the first component:the second component is in the range of 1:6 to 3.5:1.31. The composition of claim 30 , wherein the composition has a measured sucrose equivalence that is greater than the calculated sucrose equivalence of the composition.32. The composition of claim 30 , wherein the composition has a reduced bitterness or lingering compared to the second component.33. The composition of claim 30 , wherein the composition comprises less than 70% GSG by weight.34. A method of improving the taste profle or flavor of a food or beverage claim 30 , comprising:{'b': '1', 'adding an effective amount of the composition of claim to the food or beverage.'}35. The method of claim 34 , wherein the composition is present in the food or beverage at a concentration of 50-2000 ppm.36. The method of claim 34 , wherein the composition is present in the food or beverage at a final concentration of 200 ppm or less.37. The method of claim 34 , wherein the composition is present in the food or beverage at a final concentration of 100 ppm or less.38. A beverage comprising a GSG-containing composition claim 34 , wherein the GSG-containing composition is derived from a steviol glycoside (SG) composition comprising 20-99.5% RA by weight and wherein the GSG-containing composition is present in the beverage in a concentration of 200 ppm or less claim 34 , or in an amount that produces a calculated sweetness of 2% SE or less.39. The beverage of claim 38 , wherein the GSG-containing ...

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Номер записи: 68
23-03-2017 дата публикации

RECOMBINANT PRODUCTION OF STEVIOL GLYCOSIDES

Номер: US20170081691A1
Автор: Mao Guohong, Yu Xiaodan
Принадлежит: Conagen Inc.

Recombinant polypeptides having UDP-glycosyltransferase activities, including a 1,2-19-O-glucose glycosylation activity and a 1,2-13-O-glucose glycosylation activity for synthesizing of steviol glucosides, are provided. A method of producing a steviol glycoside composition using such recombinant polypeptide is also provided. Also disclosed are steviol glycosides referred to as rebaudioside Z1 and rebaudioside Z2. 111.-. (canceled)12. A method of producing a steviol glycoside composition , the method comprising incubating a substrate with a recombinant polypeptide comprising an amino acid sequence having at least 80% identity to SEQ ID NO:6.13. The method of claim 12 , further comprising incubating a recombinant sucrose synthase with the substrate and the recombinant polypeptide.14. The method of claim 13 , wherein the recombinant sucrose synthase comprises an amino acid sequence having at least 80% identity to SEQ ID NO:9.15. The method of claim 13 , further comprising incubating a recombinant UDP-glycosyltransferase with the sucrose synthase claim 13 , the substrate claim 13 , and the recombinant polypeptide.16. The method of claim 15 , wherein the recombinant UDP-glycosyltransferase comprises an amino acid sequence having at least 80% identity to SEQ ID NO:11.17. The method of claim 12 , wherein the substrate is selected from the group consisting of stevioside claim 12 , rebaudioside A claim 12 , rebaudioside E claim 12 , and combinations thereof.1820.-. (canceled)21. A method of producing rebaudioside Z claim 12 , the method comprising incubating a substrate with a recombinant polypeptide comprising an amino acid sequence having at least 80% identity to SEQ ID NO:622. The method of claim 21 , wherein the substrate is selected from the group consisting of rubusoside claim 21 , stevioside claim 21 , and combinations thereof.23. The method of claim 21 , further comprising incubating a recombinant sucrose synthase with the substrate and the recombinant polypeptide.24 ...

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Номер записи: 69
12-03-2020 дата публикации

STEVIA SWEETENER AND MANUFACTURING PROCESS

Номер: US20200080120A1
Автор: LAI Yuqiang, LIN Dongdong, ZHANG Laiwang
Принадлежит:

A method of preparing a glucosylated steviol glycoside composition involving by treating stevia materials with cyclodextrin glycosyltransferase. 1Stevia. A process for producing a highly purified glucosyl composition , comprising the steps of:{'i': 'Stevia rebaudiana', '(a) providing steviol glycosides extracted from in an acidic solution, thereby forming an acidic mixture;'}(b) contacting the acidic mixture with a first amount of cyclodextrin glycosyltransferase to form a first enzyme treated mixture;(c) maintaining the first enzyme treated mixture at a temperature of about 65° C. for between 4 and 10 hours;(d) contacting the first enzyme treated mixture with a second amount of cyclodextrin glycosyltransferase to form a second enzyme treated mixture;(e) maintaining the second enzyme treated mixture at a temperature of about 65° C. for between 6 and 10 hours;(f) inactivating the cyclodextrin glycosyltransferase in the second enzyme treated mixture by raising the temperature of the second enzyme treated mixture to between about 90° C. and about 100° C., thereby forming an enzyme inactivated mixture; and{'i': 'Stevia', '(g) purifying the enzyme inactivated mixture to form the glucosyl composition.'}2. The process of claim 1 , wherein prior to step (a) the process comprises the steps of:{'i': 'Stevia rebaudiana', '(i) soaking materials in water to extract steviol glycosides, thereby forming an aqueous mixture;'}(ii) passing the aqueous mixture over a column containing activated charcoal, thereby adsorbing steviol glycosides on the activated charcoal; and(iii) eluting the adsorbed steviol glycosides from the activated charcoal.3. The process of claim 1 , wherein the acidic mixture of step (a) is at a temperature of about 65° C.4. The process of claim 1 , wherein the acidic mixture comprises about 15 weight percent steviol glycosides.5. The process of claim 1 , wherein the acidic mixture comprises about 15 weight percent maltodextrin.6. The process of claim 1 , wherein ...

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Номер записи: 70
12-03-2020 дата публикации

Production of steviol glycosides in recombinant hosts

Номер: US20200080123A1
Автор: Christian Nyffenegger, Christophe FOLLY, Joe SHERIDAN, Jon HEAL, Kim OLSSON, Laura BREMBATI, Laura OCCHIPINTI, Philipp BERNINGER
Принадлежит: Evolva AG

The invention relates to recombinant microorganisms and methods for producing steviol glycosides and steviol glycoside precursors.

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Номер записи: 71
29-03-2018 дата публикации

SWEETENER WITH IMBEDDED HIGH POTENCY INGREDIENTS AND PROCESS AND APPARATUS FOR MAKING THE SWEETENER

Номер: US20180084810A1
Автор: Zou Li Li
Принадлежит:

An enhanced sweetener made from granules of a carrier, such as sucrose, embedded with molecules of a high potency sweetener, such as extract. The process for making the enhanced sweetener involves dissolving the high potency sweetener in warmed water and mixing the resulting solution with the heated carrier. The resulting slurry is then dried, optionally under reduced pressure. The apparatus for producing the enhanced sweetener includes one or more heated agitators connected to a heated, airtight blender, which can be evacuated with a vacuum pump. The result is an enhanced sweetener with a homogenous distribution of high potency ingredients that retains the functionalities of sugar necessary for food preparation, improves the spreading of the sweetness throughout the food, and reduces aftertastes. Other high potency ingredients and carriers may also be used in the process. 1. A granule of enhanced sweetener comprising an edible carrier imbedded with molecules of a high potency sweetener.2. The granule of claim 1 , wherein the edible carrier is sucrose.3stevia. The granule of claim 1 , wherein the high potency sweetener is extract.4. The granule of claim 1 , wherein the edible carrier is further imbedded with one or more of a vitamin claim 1 , a mineral claim 1 , a flavoring and a coloring.5. An enhanced sweetener comprising a plurality of granules of enhanced sweetener claim 1 , each granule comprising an edible carrier imbedded with molecules of a high potency sweetener claim 1 , wherein the high potency sweetener is distributed homogenously between the plurality of granules.6. The enhanced sweetener of claim 5 , made bydissolving the high potency sweetener in warmed water to form a solution;heating the edible carrier;mixing the solution with the heated, edible carrier to form a slurry; anddrying the slurry.7. The granules of claim 6 , wherein the slurry is dried under reduced pressure.8. A process for making an enhanced sweetener claim 6 , comprising:dissolving a ...

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Номер записи: 72
29-03-2018 дата публикации

Guanidinoglycoside-mediated liposome-based delivery of therapeutics

Номер: US20180086782A1
Автор: Esko Jeffrey D., Tor Yitzhak
Принадлежит:

This disclosure relates to the incorporation of amphiphilic guanidinylated aminoglycosides (e.g., neomycin) into liposomal assemblies, which contain entrapped therapeutics. The lysosome is responsible for enzymatically breaking down and recycling large biomolecules and aged organelles. While malfunctioned lysosomal enzymes have been established in Lysosomal Storage Disorders (LSDs), recent reports have suggested that defects in lysosomal enzymes (e.g., glucocerebrosidase) are also linked to other chronic ailments, including neurological disorders such as Parkinson's Disease and related disorders. 1. A compound of Formula I:{'br': None, 'A-B-C\u2003\u2003I'} A is a guanidinylated neomycin derivative;', 'B is a linker group; and', 'C is a phospholipid, a fatty acid, or a fatty acid group., 'or a pharmaceutically acceptable salt thereof, wherein3. The compound of claim 2 , wherein the guanidine group is an N-protected guanidine.7. The compound of any one of to claim 2 , wherein B is a linker group comprising a linker selected from the group consisting of one or more alkylene groups claim 2 , one or more amide groups claim 2 , one or more alkyleneoxy groups claim 2 , one or more heteroaryl groups claim 2 , one or more amine groups claim 2 , or any combination thereof.8. The compound of any one of to claim 2 , wherein B is a linker group comprising one or more Calkylene groups claim 2 , one or more —(OCHCH)— or —(OCH)— groups claim 2 , one amide group claim 2 , one —NH— group claim 2 , and one 5-6 membered heteroaryl group.10. The compound of claim 9 , wherein m is an integer from 1 to 5.11. The compound of claim 9 , wherein m is 3.12. The compound of any one of to claim 9 , wherein n is an integer from 0 to 5.13. The compound of any one of to claim 9 , wherein n is 0 or 3.14. The compound of any one of to claim 9 , wherein C is a phospholipid.15. The compound of any one of to claim 9 , wherein C is a phospholipid comprising one or more choline groups claim 9 , one or ...

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Номер записи: 73
05-05-2022 дата публикации

COMPOSITION COMPRISING TRANSGLYCOSYLATED STEVIOSIDE AND TRANSGLYCOSYLATED REBAUDIOSIDE A

Номер: US20220132898A1
Автор: Chu Sun, Kang In Sung, KIM Min Hoe, PARK Sunghee, YANG Tae Joo
Принадлежит: CJ CHEILJEDANG CORPORATION

The present disclosure relates to a sweetener composition, comprising a transglycosylated Stevioside and a transglycosylated Rebaudioside A.

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Номер записи: 74
19-03-2020 дата публикации

HIGH-PURITY RUBUSOSIDE AND PROCESS FOR PRODUCING OF THE SAME

Номер: US20200087694A1
Автор: MARKOSYAN Avetik
Принадлежит:

The invention provides a process of producing Rubusoside from steviol glycosides of plant. The process is useful for producing high purity Rubusoside with purity greater than 95% (dry basis). High purity rubusoside is useful as in combination with other caloric and non-caloric sweeteners as well as non-caloric sweetener in various food and beverage compositions. The high purity rubusoside is useful as non-caloric sweetener in edible and chewable compositions such as any beverages, confectionaries, bakeries, cookies, chewing gums, and alike. 1Stevia rebaudiana. A process for preparing Rubusoside from steviol glycosides of comprising the steps of:a. providing a solution comprising Stevioside;b. providing an enzyme composition comprising at least one enzyme selected from the group comprising rhamnosidase, β-glucosidase, β-glucanase, hesperidinase, naringinase, pectinase, cellulase, hemicellulase, xylanase;c. adding the enzyme composition to the solution comprising Stevioside to obtain reaction mixture;d. incubating the reaction mixture to facilitate complete or partial transformation of Stevioside to Rubusoside;e. recovering and purifying high purity Rubusoside from reaction mixture.2. A process of wherein step (a) the concentration of Stevioside is 1-50% (wt/vol) claim 1 , preferably 5-30% (wt/vol) claim 1 , more preferably 8-10% (wt/vol).3. A process of wherein step (d) the molar yield of Rubusoside from Stevioside is 1-100% claim 1 , preferably 80-100% and more preferably 95-100%.4. A process of wherein step (e) at least one purification techniques is employed selected from the group comprising ion exchange treatment claim 1 , macroporous adsorbent treatment claim 1 , membrane process claim 1 , chromatographic separation claim 1 , and crystallization from various solvent systems.5. A process of wherein step (e) the recovery and purification of Rubusoside comprises the steps of:a. providing reaction mixture containing Rubusoside;b. contacting the reaction mixture ...

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Номер записи: 75
19-03-2020 дата публикации

BIOSYNTHETIC PRODUCTION OF STEVIOL GLYCOSIDES REBAUDIOSIDE J AND REBAUDIOSIDE N

Номер: US20200087695A1
Автор: Batten Michael, Hunt Phillip, Mao Guohong, Yu Oliver (Xiaodan)
Принадлежит: Conagen Inc.

The present disclosure relates to the production of steviol glycosides rebaudioside J and rebaudioside N through the use of rebaudioside A as a substrate and a biosynthetic pathway involving various 1,2 RhaT-rhamnosyltransferases. 1. A biosynthetic method of preparing rebaudioside N , the method comprising:reacting a steviol glycoside composition with a rhamnose donor moiety in the presence of a first recombinant polypeptide having 1,2-rhamnosytransferase activity; wherein said first recombinant polypeptide comprises an amino acid sequence having at least 80% sequence identity to SEQ ID NO: 3.2. The method of claim 1 , wherein said first recombinant polypeptide comprises an amino acid sequence having at least 90% sequence identity to SEQ ID NO: 3.34.-. (canceled)5. The method of claim 1 , comprising expressing said first recombinant polypeptide in a transformed cellular system.6. The method of claim 5 , wherein the transformed cellular system is selected from the group consisting of a yeast claim 5 , a non-steviol glycoside producing plant claim 5 , an alga claim 5 , a fungus claim 5 , and a bacterium.7. (canceled)8. The method of claim 5 , wherein the reacting step is performed in the transformed cellular system.9. The method of claim 5 , comprising isolating said first recombinant polypeptide from the transformed cellular system and the reacting step is performed in vitro.10. The method of claim 1 , wherein the rhamnose donor moiety is UDP-L-rhamnose.11. The method of claim 1 , wherein the steviol glycoside composition comprises rebaudioside A and the reacting step leads to the production of rebaudioside J.12. The method of claim 11 , comprising reacting said rebaudioside J with a glucose donor moiety in the presence of a second recombinant polypeptide having glucosytransferase activity.13. The method of claim 12 , wherein the glucose donor moiety is generated in situ.14. The method of claim 12 , wherein said second recombinant polypeptide has both ...

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Номер записи: 76
07-04-2016 дата публикации

NON-CALORIC SWEETENERS AND METHODS FOR SYNTHESIZING

Номер: US20160097070A1
Автор: Lin Ying, Luo Yang, Mao Guohong, Yu Xiaodan
Принадлежит:

Disclosed are steviol glycosides referred to as rebaudioside V and rebaudioside W. Also disclosed are methods for producing rebaudioside M (Reb M), rebausoside G (Reb G), rebaudioside KA (Reb KA), rebaudioside V (Reb V) and rebaudioside (Reb W). 1. A method for synthesizing a mixture stevioside and rebaudioside KA from rubusoside , the method comprising:preparing a reaction mixture comprising rubusoside, substrates selected from the group consisting of sucrose, uridine diphosphate (UDP) and uridine diphosphate-glucose (UDP-glucose), a UDP-glycosyltransferase selected from the group consisting of EUGT11 and a UDP-glycosyltransferase fusion enzyme; andincubating the reaction mixture for a sufficient time to produce a mixture of stevioside and rebaudioside KA, wherein a glucose is covalently coupled to C2′-19-O-glucose of rubusoside to produce rebaudioside KA, and a glucose is convalently coupled to C2′-13-O-glucose of rubusoside to produce stevioside.2. The method of further comprising adding a sucrose synthase to the reaction mixture.3ArabidopsisArabidopsisVigna radiate. The method of claim 2 , wherein the sucrose synthase is selected from the group consisting of an sucrose synthase 1; an sucrose synthase 3; and a sucrose synthase.4Arabidopsis thaliana. The method of claim 3 , wherein the sucrose synthase is an sucrose synthase 1.5. The method of claim 1 , wherein the UDP-glycosyltransferase fusion enzyme is selected from the group consisting of an EUGT11 uridine diphospho glycosyltransferase domain coupled to a sucrose synthase domain.6ArabidopsisArabidopsisVigna radiate. The method of claim 5 , wherein the sucrose synthase domain is selected from the group consisting of an sucrose synthase 1; an sucrose synthase 3; and a sucrose synthase.7Arabidopsis thaliana. The method of claim 6 , wherein the sucrose synthase domain is an sucrose synthase 1.8. A method for synthesizing rebaudioside KA from rubusoside claim 6 , the method comprising:preparing a reaction mixture ...

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Номер записи: 77
07-04-2016 дата публикации

NON-CALORIC SWEETENERS AND METHODS FOR SYNTHESIZING

Номер: US20160097071A1
Автор: Lin Ying, Luo Yang, Mao Guohong, Yu Xiaodan
Принадлежит:

Disclosed are steviol glycosides referred to as rebaudioside V and rebaudioside W. Also disclosed are methods for producing rebaudioside M (Reb M), rebausoside G (Reb G), rebaudioside KA (Reb KA), rebaudioside V (Reb V) and rebaudioside (Reb W). 3. A method for synthesizing rebaudioside V from rebaudioside G , the method comprising:preparing a reaction mixture comprising rebaudioside G, substrates selected from the group consisting of sucrose, uridine diphosphate (UDP) and uridine diphosphate-glucose (UDP-glucose), a HV1 UDP-glycosyltransferase; andincubating the reaction mixture for a sufficient time to produce rebaudioside V, wherein a glucose is covalently coupled to the rebaudioside G to produce rebaudioside V.4. The method of further comprising adding a sucrose synthase to the reaction mixture.5ArabidopsisArabidopsisVigna radiate. The method of claim 3 , wherein the sucrose synthase is selected from the group consisting of an sucrose synthase 1; an sucrose synthase 3; and a sucrose synthase.6Arabidopsis thaliana. The method of claim 5 , wherein the sucrose synthase is an sucrose synthase 1.7. A method for synthesizing rebaudioside V from rebaudioside G claim 5 , the method comprising:preparing a reaction mixture comprising rebaudioside G, substrates selected from the group consisting of sucrose, uridine diphosphate (UDP) and uridine diphosphate-glucose (UDP-glucose), and a uridine dipospho glycosyltransferase (UDP-glycosyltransferase) selected from the group consisting of an EUGT11 and a UDP-glycosyltransferase fusion enzyme; andincubating the reaction mixture for a sufficient time to produce rebaudioside V, wherein a glucose is covalently coupled to the rebaudioside G to produce rebaudioside V.8. The method of further comprising adding a sucrose synthase to the reaction mixture.9ArabidopsisArabidopsisVigna radiate. The method of claim 8 , wherein the sucrose synthase is selected from the group consisting of an sucrose synthase 1; an sucrose synthase 3; and a ...

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Номер записи: 78
07-04-2016 дата публикации

NON-CALORIC SWEETENERS AND METHODS FOR SYNTHESIZING

Номер: US20160097072A1
Автор: Lin Ying, Luo Yang, Mao Guohong, Yu Xiaodan
Принадлежит:

Disclosed are steviol glycosides referred to as rebaudioside V and rebaudioside W. Also disclosed are methods for producing rebaudioside M (Reb M), rebaudioside G (Reb G), rebaudioside KA (Reb KA), rebaudioside V (Reb V) and rebaudioside (Reb W). 1. A method for synthesizing rebaudioside E from rebaudioside KA , the method comprising:preparing a reaction mixture comprising rebaudioside KA, substrates selected from the group consisting of sucrose, uridine diphosphate (UDP) and uridine diphosphate-glucose (UDP-glucose), and a UDP-glycosyltransferase selected from the group consisting of HV1, EUGT11 and a UDP-glycosyltransferase fusion enzyme; andincubating the reaction mixture for a sufficient time to produce rebaudioside E, wherein a glucose is covalently coupled to the C2′ 13-O-glucose of rebaudioside KA to produce rebaudioside E.2. The method of further comprising adding a sucrose synthase to the reaction mixture.3ArabidopsisArabidopsisVigna radiate. The method of claim 2 , wherein the sucrose synthase is selected from the group consisting of an sucrose synthase 1; an sucrose synthase 3; and a sucrose synthase.4Arabidopsis thaliana. The method of claim 3 , wherein the sucrose synthase is an sucrose synthase 1.5. The method of claim 1 , wherein the UDP-glycosyltransferase fusion enzyme is selected from the group consisting of an EUGT11 uridine diphospho glycosyltransferase domain coupled to a sucrose synthase domain.6ArabidopsisArabidopsisVigna radiate. The method of claim 5 , wherein the sucrose synthase domain is selected from the group consisting of an sucrose synthase 1; an sucrose synthase 3; and a sucrose synthase.7Arabidopsis thaliana. The method of claim 6 , wherein the sucrose synthase domain is an sucrose synthase 1.814.-. (canceled)15. A method for synthesizing rebaudioside D2 from rubusoside claim 6 , the method comprising:preparing a reaction mixture comprising rubusoside, substrates selected from the group consisting of sucrose, uridine diphosphate ( ...

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Номер записи: 79
01-04-2021 дата публикации

HIGH-PURITY STEVIOL GLYCOSIDES

Номер: US20210095322A1
Автор: Afzaal Bin Hasim Mohamad, Chow Siew Yin, MARKOSYAN Avetik, Nizam Bin Nawi Khairul, Petit Marcia, PURKAYASTHA Siddhartha, Ramandach Saravanan A/L
Принадлежит: PURECIRCLE USA INC.

Methods of using highly purified rebaudioside AM are described. The methods include utilizing enzyme preparations and recombinant microorganisms for converting various staring compositions to target steviol glycosides. The highly purified rebaudioside AM is useful as flavor enhancer, sweetness enhancer, and foaming suppressor in edible and chewable compositions such as any beverages, confectioneries, bakery products, cookies, and chewing gums. 2. A method for producing the highly purified rebaudioside AM of claim 1 , comprising the steps of:a. providing a starting composition comprising an organic compound with at least one carbon atom;b. providing a biocatalyst selected from the group consisting of an enzyme preparation, a cell or a microorganism; said biocatalyst comprising at least one enzyme capable of converting the starting composition to rebaudioside AM;c. contacting the biocatalyst with a medium containing the starting composition to produce a medium comprising rebaudioside AM3. The method of further comprising the step of:d. separating the rebaudioside AM from the medium to provide a highly purified rebaudioside AM composition.4. The method of claim 2 , wherein the starting composition is selected from the group consisting of steviol claim 2 , steviolmonoside claim 2 , steviolmonoside A claim 2 , steviolbioside claim 2 , steviolbioside A claim 2 , steviolbioside B claim 2 , rubusoside claim 2 , stevioside claim 2 , stevioside A (rebaudioside KA) claim 2 , stevioside B claim 2 , stevioside C claim 2 , rebaudioside E claim 2 , rebaudioside E2 claim 2 , rebaudioside E3 claim 2 , other steviol glycosides claim 2 , polyols claim 2 , carbohydrates claim 2 , and combinations thereof.5E. coli, SaccharomycesAspergillusPichiaBacillusYarrowia. The method of claim 2 , wherein the microorganism is selected from the group consisting of sp. claim 2 , sp. claim 2 , sp. claim 2 , sp. claim 2 , and sp.6. The method of claim 2 , wherein the enzyme is selected from the group ...

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Номер записи: 80
26-03-2020 дата публикации

NATURALLY SWEET ENHANCER COMPOSITION

Номер: US20200093165A1
Автор: Eidenberger Thomas, Shi Jingang, Wang Hansheng
Принадлежит:

The invention describes the use of one or more steviol glycosides (SGs) having a molecular weight (daltons) of less than or equal to about 965 to improve sweetness profiles of compositions, including sweet tea extracts, stevia extracts, swingle extracts, glycosylated sweet tea extracts, glycosylated stevia extracts, glycosylated swingle extracts, glycosylated sweet tea glycosides, glycosylated steviol glycosides, glycosylated mogrosides, as well as the individual components of the extracts or glycosylated products, and mixtures thereof. 1. A composition comprising one or more of rubusoside (RU) , steviolmonoside (STM) or steviolbioside (STB) with rebaudioside A (RA) and rebaudioside B (RB) , wherein the composition has a reduced sweet lingering aspect that is less than that of the RA/RB combination without the RU , STM or STB present.2. The composition of claim 1 , wherein the combination of RA to RB is from about 10:90 to about 90:10 by weight.3. The composition of claim 2 , wherein the combination of RA to RB is about 75:15 by weight.4. The composition of claim 1 , wherein the ratio of the RU claim 1 , STM or STB is about 1:4 by weight relative to the weight of the RA/RB combination.5. The composition of claim 1 , wherein the combination of RA to RB is about 75:15 by weight and the ratio of the RU claim 1 , STM or STB is about 1:4 by weight of the weight of the RA/RB combination.6. A composition comprising one or more of rebaudioside A (RA) claim 1 , rebaudioside B (RB) or rebaudioside D (RD) with rubusoside (RU) claim 1 , wherein a sweet lingering aspect of the composition is decreased in comparison to that of the RA claim 1 , RB or RD without the RU present.7. The composition of claim 6 , wherein the ratio of RA claim 6 , RB claim 6 , or RD to RU is about 20:1 to about 5:1.8. The composition of claim 7 , wherein the ratio of RA claim 7 , RB or RD to RU is about 9:1 by weight.9. A composition comprising thaumatin and one or more of rubusoside (RU) or ...

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Номер записи: 81
14-04-2016 дата публикации

EXTRACELLULAR DITERPENE PRODUCTION

Номер: US20160102331A1
Автор: BOER Viktor Marius, BROERS Nicolette Jasmijn, LAWRENCE Adam G.
Принадлежит:

The present invention relates to a method for the production of a diterpene or a glycosylated diterpene, which method comprises: a. fermenting a recombinant microorganism in a suitable fermentation medium, wherein the microorganism comprises one or more nucleotide sequence(s) encoding: a polypeptide having ent-copalyl pyrophosphate synthase activity; a polypeptide having ent-Kaurene synthase activity; a polypeptide having ent-Kaurene oxidase activity; and a polypeptide having kaurenoic acid 13-hydroxylase activity and whereby expression of the nucleotide sequence(s) confer(s) on the microorganism the ability to produce at least steviol, whereby a diterpene or glycosylated diterpene is produced extracellularly in the fermentation medium; and b. recovering the diterpene or glycosylated diterpene from the fermentation medium. 1. A method for the production of a diterpene or a glycosylated diterpene , which method comprises:a. fermenting a recombinant microorganism in a suitable fermentation medium,wherein the microorganism comprises one or more nucleotide sequence(s) encoding: a polypeptide having ent-copalyl pyrophosphate synthase activity; a polypeptide having ent-Kaurene synthase activity; a polypeptide having ent-Kaurene oxidase activity; and a polypeptide having kaurenoic acid 13-hydroxylase activity and whereby expression of the nucleotide sequence(s) confer(s) on the microorganism the ability to produce at least steviol,whereby a diterpene or glycosylated diterpene is produced extracellularly in the fermentation medium; andb. recovering the diterpene or glycosylated diterpene from the fermentation medium.2. A method according to claim 1 , wherein the recombinant microorganism comprises one or more nucleotide sequences encoding a polypeptide having UDP-glucosyltransferase activity claim 1 ,whereby expression of the nucleotide sequence(s) confer(s) on the microorganism the ability to produce at least one of steviolmonoside, steviolbioside, stevioside or ...

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Номер записи: 82
12-04-2018 дата публикации

HIGH-PURITY STEVIOL GLYCOSIDES

Номер: US20180100175A1
Автор: Badie Aurelien, Bunders Cynthia, Cyrille Jarrin, MARKOSYAN Avetik, Prakash Indra, Soni Pankaj, ter HALLE Robert
Принадлежит:

Methods of preparing highly purified steviol glycosides, particularly rebaudiosides A, D and M are described. The methods include utilizing recombinant microorganisms for converting various staring compositions to target steviol glycosides. In addition, novel steviol glycosides reb D2 and reb M2 are disclosed, as are methods of preparing the same. The highly purified rebaudiosides are useful as non-caloric sweetener in edible and chewable compositions such as any beverages, confectioneries, bakery products, cookies, and chewing gums. 1. A method for producing highly purified target steviol glycosides selected from the group consisting of reb M , reb M2 , reb D2 , and mixtures thereof , comprising the steps of:a. providing an aqueous solution comprising a starting composition comprising steviol glycosides;b. providing a microorganism containing at least one enzyme selected from the group consisting of steviol biosynthesis enzymes, UDP-glycosyltransferases, UDP-glucose recycling enzymes, and a combination thereof;c. contacting the microorganism containing the enzyme with a medium containing the starting composition to produce a medium comprising at least one target steviol glycoside.2. (canceled)3. The method of further comprising the step of:d. separating the target steviol glycoside from the medium to provide a highly purified target steviol glycoside composition.4. (canceled)5E. coli, SaccharomycesAspergillusPichiaBacillusYarrowia. The method of claim 1 , wherein the enzyme is contained in a microorganism selected from the group consisting of sp. claim 1 , sp. claim 1 , sp. claim 1 , sp. claim 1 , and sp.6. The method of claim 1 , wherein the enzyme is provided as a biocatalyst selected from the group consisting of whole cell suspension claim 1 , crude lysate or purified enzymes in free or immobilized form.7. The method of claim 1 , wherein the target steviol glycoside is selected from the group consisting of stevioside claim 1 , reb A claim 1 , reb D claim 1 , reb ...

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Номер записи: 83
08-04-2021 дата публикации

Fermentation methods for producing steviol glycoside using high ph and compositions obtained therefrom

Номер: US20210102229A1
Автор: Ariene M. Fosmer, Hans Peter Smits, James C. Anderson, Manuel Quiros ASENSIO, Simon CARLSEN, Ting Liu Carlson, Véronique DOUCHIN
Принадлежит: Cargill Inc, Evolva AG

Disclosed are methods for producing steviol glycosides, such as rebaudioside D and rebaudioside M, using engineered yeast. In some embodiments, the methods include fermenting with a yeast at a high pH such as of about 5.8 or greater. In some embodiments, the methods can be carried out by first growing the yeast at a lower first pH, and then adjusting the pH to a higher pH.

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Номер записи: 84
02-06-2022 дата публикации

METHODS OF PRODUCING MOGROSIDES AND COMPOSITIONS COMPRISING SAME AND USES THEREOF

Номер: US20220170063A1
Автор: BURGER Yosef, COHEN Shahar, DAVIDOVICH-RIKANATI Rachel, DORON-FAIGENBOIM Adi, ITKIN Maxim, KATZIR Nurit, LEWINSOHN Efraim, Oren Elad, Petreikov Marina, Portnoy Vitaly, Schaffer Arthur A., SHEN Shmuel, TADMOR Yaakov
Принадлежит: The State of Israel, Ministry of Agriculture & Rural Development, Agricultural Research Organization

Isolated mogroside and mogrol biosynthetic pathway enzyme polypeptides useful in mogroside biosynthesis are provided. Mogroside biosynthetic pathway enzymes of the invention include squalene epoxidase (SE), epoxy hydratase (EH), cytochrome p450 (Cyp), cucurbitadienol synthase (CDS) and udp-glucosyl-transferase (UGT), Also provided are methods of producing a mogroside using the isolated mogroside and mogrol biosynthetic enzyme polypeptides, the methods comprising contacting a mogrol and/or a glycosylated mogrol (mogroside) with at least one UDP glucose glucosyl transferase (UGT) enzyme polypeptide of the invention catalyzing glucosylation of the mogrol and/or the glucosylated mogrol to produce a mogroside with an additional glucosyl moietie(s), thereby producing the mogroside. Alternatively or additionally provided is a method of synthesizing a mogrol, the method comprising contacting a mogrol precursor substrate with one or more mogrol biosynthetic pathway enzyme polypeptides as described herein catalyzing mogrol synthesis from the mogrol precursor substrate, thereby synthesizing the mogrol. 1. A method of synthesizing a mogrol or mogrol precursor product from a mogrol precursor substrate , the method comprising contacting at least one mogrol precursor substrate with a mogroside pathway enzyme , wherein:(a) when said mogrol precursor product comprises diepoxy squalene and said mogrol precursor substrate comprises squalene or oxidosqualene, said mogroside pathway enzyme comprises a squalene epoxidase polypeptide at least 94% identical to SEQ ID NO: 14 or 89% identical to SEQ ID NO: 16, wherein said polypeptide catalyzes diepoxysqualene synthesis from squalene or oxidosqualene, thereby producing diepoxy squalene,(b) when said mogrol precursor product comprises 3 hydroxy, 24-25 epoxy cucurbitadienol and said mogrol precursor substrate comprises diepoxy squalene, said mogrol pathway enzyme comprises a cucurbitadienol synthetase polypeptide at least 60% homologous or ...

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Номер записи: 85
19-04-2018 дата публикации

Methods for Chemical Synthesis of Biologically Active Compounds Using Supramolecular Protective Groups and Novel Compounds Obtainable Thereby

Номер: US20180105546A1
Автор: Bastian Andreas Alexander, Herrmann Andreas, Marcozzi Alessio
Принадлежит: Rijksuniversiteit Groningen

The invention relates to drug development and synthetic chemistry, in particular to the manufacture of biologically active compounds based on naturally occurring molecules. It also relates to novel biologically active compounds, for example aminoglycoside antibiotics, in a substantially pure regioisomeric form. More particularly, the present invention relates to methods for the chemo- or regioselective derivatization of a target compound with multiple reactive groups, some of which may be derivatezed, and other which will not be derivatized. 1. A method for the chemo- and/or regio selective derivatization of a target compound comprising multiple chemically equivalent reactive groups , wherein at least one reactive group is to be derivatized and wherein at least one reactive group is not to be derivatized , the method comprising the steps ofa. contacting the target compound with at least one non-covalent protective group under conditions allowing for the formation of a regioselective host-guest complex, wherein the protective group is an oligonucleotide or oligopeptide aptamer having a selective affinity for the at least one reactive group not to be modified; followed and/or accompanied byb. chemical derivatization of the target compound.2. Method according to claim 1 , wherein the target compound comprises at least three chemically equivalent reactive groups.3. Method according to or claim 1 , wherein the chemically equivalent reactive groups are amines claim 1 , hydroxyls claim 1 , hydroxylamines claim 1 , carboxylic acids claim 1 , thiols claim 1 , aldehydes claim 1 , ketones claim 1 , enamines claim 1 , C—C double bonds or C—C triple bonds.4. Method according to any one of the preceding claims claim 1 , wherein the target compound is a biologically active compound.5. Method according to any one of the preceding claims claim 1 , wherein the target compound is a proteinaceous substance.6. Method according to any one of to claim 1 , wherein the target compound is a ...

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Номер записи: 86
02-04-2020 дата публикации

BIOSYNTHETIC PRODUCTION OF STEVIOL GLYCOSIDE REBAUDIOSIDE D4 FROM REBAUDIOSIDE E

Номер: US20200102588A1
Автор: Batten Michael, Luo Yang, Mao Guohong, Vick Jacob Edward, Wu Yilin, Yu Xiaodan
Принадлежит: Conagen Inc.

The present invention relates, in some aspects, to the production of steviol glycoside rebaudioside D4 through the use of rebaudioside E. In some aspects, the invention relates to mutant CP1 enzymes, mutant HV1 enzymes as well as host cells and methods utilizing such enzymes, such as to produce rebaudioside D4. 121.-. (canceled)22. A method of producing rebaudioside D4 , the method comprising incubating a substrate with a recombinant polypeptide comprising an amino acid sequence having at least 80% identity to SEQ ID NO 7 or SEQ ID NO: 9.23. The method of claim 22 , wherein the substrate is selected from the group consisting of Reb E claim 22 , stevioside claim 22 , and combinations thereof.24. The method of claim 23 , further comprising incubating a recombinant sucrose synthase with the substrate and the recombinant polypeptide.25. A method for synthesizing rebaudioside D4 from rebaudioside E claim 23 , the method comprising:preparing a reaction mixture comprising rebaudioside E, a substrate selected from the group consisting of sucrose, uridine diphosphate (UDP) and uridine diphosphate glucose (UDP-glucose), and C1m2, andincubating the reaction mixture for a sufficient time to produce rebaudioside D4, wherein a glucose is covalently coupled to the rebaudioside E to produce rebaudioside D4.2628-. (canceled)29. A method for synthesizing rebaudioside D4 from rebaudioside E claim 23 , the method comprising:preparing a reaction mixture comprising rebaudioside E, a substrate selected from the group consisting of sucrose, uridine diphosphate (UDP) and uridine diphosphate glucose (UDP-glucose), and C1m3, andincubating the reaction mixture for a sufficient time to produce rebaudioside D4, wherein a glucose is covalently coupled to the rebaudioside E to produce rebaudioside D4.3033.-. (canceled)34. The method of further comprising adding a HV1 enzyme to the reaction mixture.35. The method of further comprising adding a HV1 enzyme to the reaction mixture.3644.-. (canceled)45 ...

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Номер записи: 87
02-04-2020 дата публикации

METHODS FOR PRODUCING REBAUDIOSIDE D AND REBAUDIOSIDE M AND COMPOSITIONS THEREOF

Номер: US20200102589A1
Автор: Hua Jun, Pei Liang, Wang Yong
Принадлежит: SICHUAN INGIA BIOSYNTHETIC CO., LTD.

The invention relates to methods for producing rebaudioside D and/or rebaudioside M, and compositions comprising the same. The invention provides a method for producing RD and/or RM compositions. The method comprises using rebaudioside A and/or stevioside as substrate and a recombinant microorganism or an enzyme produced by the recombinant microorganism or a metabolite of the recombinant microorganism to catalyze the reaction of the substrate in the presence of sucrose and trisodium citrate and produce a mixture of rebaudioside D and rebaudioside M, and then separates and purifies the mixture to obtain rebaudioside D or rebaudioside M. 1. A method for producing rebaudioside D and/or rebaudioside M , comprising:providing a starting composition comprising at least one of rebaudioside A and stevioside;incubating the starting composition with a recombinant microorganism in a mixture, wherein the recombinant microorganism expresses an EUGT11 enzyme and a UGT76G1 enzyme; andpurifying rebaudioside D and/or rebaudioside M from the mixture.2. A method for producing rebaudioside D and/or rebaudioside M , comprising:providing a starting composition comprising at least one of rebaudioside A and stevioside;incubating the starting composition with an enzyme preparation produced by a recombinant microorganism in a mixture, wherein the recombinant microorganism expresses an EUGT11 enzyme and a UGT76G1 enzyme;purifying rebaudioside D and/or rebaudioside M from the mixture.3. The method of claim 1 , wherein the incubating step is performed in the presence of sucrose and trisodium citrate.4. The method of claim 2 , wherein the incubating step is performed in the presence of sucrose and trisodium citrate.5Escherichia coliPichia pastorisBacillus subtilisCorynebacterium glutamicum. The method of claim 3 , wherein the recombinant microorganism is recombinant claim 3 , recombinant claim 3 , recombinant claim 3 , recombinant or recombinant streptomycete.6Escherichia coliPichia ...

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Номер записи: 88
09-06-2022 дата публикации

COMPOSITIONS COMPRISING MOGROSIDES, STEVIOL GLYCOSIDES AND GLYCOSYLATED DERIVATIVES THEREOF AND METHODS OF ENHANCING THE MOUTHFEEL OR SWEETNESS OF CONSUMABLES

Номер: US20220175002A1
Автор: Eidenberger Thomas, Shi Jingang, Shi Weiyao, Wang Hansheng, ZHANG Xiaorui
Принадлежит:

The invention describes compositions comprising glycosylated mogroside(s) or glycosylated swingle extracts and methods of enhancing the mouthfeel, sweetness or flavor of consumable products. 1. A sweetener or flavoring composition , comprising:(A) one or more glycosylated mogrosides (GMGs); and(B) one or more non-mogroside sweeteners.2. The sweetener or flavoring composition composition of claim 1 , wherein the one or more GMGs comprise a glycosylated mogroside IIe claim 1 , a glycosylated mogroside III claim 1 , a glycosylated mogroside IIIe claim 1 , a glycosylated mogroside IV claim 1 , a glycosylated mogroside IVe claim 1 , a glycosylated mogroside V claim 1 , a glycosylated mogroside VI claim 1 , a glycosylated siamenoside I claim 1 , a glycosylated 11-oxomogroside V claim 1 , or any combination thereof.3. The sweetener or flavoring composition of claim 2 , wherein the the one or more GMGs comprises glycosylated mogroside V.4. The sweetener or flavoring composition of claim 3 , wherein the glycosylated mogroside V comprises MGV-G1 claim 3 , MGV-G2 claim 3 , MGV-G3 claim 3 , MGV-G4 and/or MGV-G5.5. The sweetener or flavoring composition of claim 1 , wherein the one or more non-mogroside sweeteners comprise steviol glycosides.6. The sweetener or flavoring composition of claim 1 , wherein the one or more non-mogroside sweeteners comprise RD claim 1 , RM or both RD and RM.7. The sweetener or flavoring composition of claim 1 , wherein the one or more non-mogroside sweeteners comprise RU.8. The sweetener or flavoring composition of claim 1 , wherein the one or more non-mogroside sweeteners comprise glycosylated steviol glycosides (GSGs).9. The sweetener or flavoring composition of claim 1 , wherein the one or more non-mogroside sweeteners comprise glycosylated RU.10. The sweetener or flavoring composition of claim 1 , wherein the weight ratio of A:B is in the range of 99:1 to 1:99.11. The sweetener or flavoring composition of claim 1 , further comprising one or more ...

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Номер записи: 89
27-04-2017 дата публикации

HIGH-PURITY STEVIOL GLYCOSIDES

Номер: US20170112176A1
Автор: JARRIN Cyrille, MARKOSYAN Avetik, PRAKASH CHATURVEDULA Venkata Sai, Prakash Indra, ROBE Patrick, ter Halle Rob
Принадлежит:

Methods of preparing highly purified steviol glycosides, particularly rebaudiosides A, D and X are described. The method includes expression of UDP-glucosyltransferases from Bertoni, which are capable converting certain steviol glycosides to rebaudiosides A, D and X. The highly purified rebaudiosides A, D and X, are useful as non-caloric sweetener in edible and chewable compositions such as any beverages, confectioneries, bakery products, cookies, and chewing gums. 126-. (canceled)27. A method for adding at least one glucose unit to a steviol glycoside substrate to provide a target steviol glycoside , comprising contacting the steviol glycoside substrate with a recombinant biocatalyst protein enzyme comprising UDP-glucosyltransferase , wherein the target steviol glycoside is Rebaudioside X.28. The method of claim 27 , wherein the steviol glycoside substrate is selected from the group consisting of steviolmonoside claim 27 , steviolbioside claim 27 , rubusoside claim 27 , rebaudioside E claim 27 , rebaudioside G claim 27 , rebaudioside I claim 27 , stevioside claim 27 , rebaudioside A claim 27 , rebaudioside D claim 27 , synthetic steviol glycosides and combinations thereof.29. The method of claim 27 , further comprising the step of purifying the Rebaudioside X to a purity of greater than about 80% by weight.30. The method of claim 27 , further comprising the step of purifying the Rebaudioside X to a purity of greater than about 90% by weight.31. The method of claim 27 , further comprising the step of purifying the Rebaudioside X to a purity of greater than about 95% by weight.32. The method of claim 27 , wherein the UDP-glucosyltransferase is expressed in a host microorganism.33Escherichia coli, Saccharomyces species, AspergillusPischia. The method of claim 32 , wherein the host microorganism is selected from the group consisting of: species claim 32 , and species. The present invention relates to a biocatalytic process for preparing compositions comprising steviol ...

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Номер записи: 90
03-05-2018 дата публикации

COMPOSITIONS COMPRISING MOGROSIDES, STEVIOL GLYCOSIDES AND GLYCOSYLATED DERIVATIVES THEREOF AND METHODS OF ENHANCING THE MOUTHFEEL OR SWEETNESS OF CONSUMABLES

Номер: US20180116266A1
Автор: Jackson Mel Clinton
Принадлежит:

The invention describes compositions comprising glycosylated mogroside(s) or glycosylated swingle extracts and methods of enhancing the mouthfeel, sweetness or flavor of consumable products. 1. A composition comprising a glycosylated mogroside or a glycosylated swingle extract and at least one non-mogroside based sweetener , a non-swingle based sweetener or a non-stevia based sweetener.2. The composition according to claim 1 , wherein the non-mogroside based sweetener claim 1 , the non-swingle based sweetener or the non-stevia based sweetener comprises one or more of cane sugar claim 1 , beet sugar claim 1 , honey claim 1 , sucrose claim 1 , fructose claim 1 , maltose claim 1 , xylitol claim 1 , sorbitol claim 1 , dextrose claim 1 , glucose claim 1 , mannitol claim 1 , arabinose claim 1 , galactose claim 1 , mannose claim 1 , rhamnose claim 1 , xylose claim 1 , inulin claim 1 , sucralose claim 1 , aspartame claim 1 , stevia acesulfame-K claim 1 , neotame claim 1 , thaumatin claim 1 , brazzein claim 1 , miraculin claim 1 , curculin claim 1 , pentadin claim 1 , mabinlin claim 1 , erythritol claim 1 , trehalose claim 1 , raffinose claim 1 , cellobiose claim 1 , tagatose claim 1 , DOLCIA PRIMA™ allulose claim 1 , inulin claim 1 , N—[N-[3-(3-hydroxy-4-methoxyphenyl)propyl]-alpha-aspartyl]-L-phenylalanine 1-methyl ester claim 1 , glycyrrhizin claim 1 , monellin claim 1 , or sodium cyclamate and mixtures thereof.3. The composition of claim 1 , wherein the glycosylated mogroside or the glycosylated swingle extract comprises a glycosylated mogroside II claim 1 , a glycosylated mogroside III claim 1 , a glycosylated mogroside IV claim 1 , a glycosylated mogroside V claim 1 , a glycosylated siamenoside I or a glycosylated 11-oxomogroside V or any combination thereof.4. The composition of claim 3 , wherein the glycosylated mogroside or the glycosylated swingle extract comprises glycosylated mogroside V.5. The composition of claim 4 , wherein the glycosylated mogroside V is GMG- ...

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Номер записи: 91
16-04-2020 дата публикации

SWEETENER COMPOSITIONS

Номер: US20200113217A1
Автор: CHATURVEDULA VENKATA SAI PRAKASH, May James A., May Michael Perry
Принадлежит: JAMES AND CAROL MAY FAMILY, LLLP

Various embodiments of the present technology provide compositions and methods for a sweetener composition comprising leaf extract, glycosides, and/or Luo Han Guo fruit extract. The sweetener composition provides enhanced taste and/or flavor properties as compared to other non-caloric or low-caloric sweeteners. The sweetener composition has a reduced aftertaste relative to leaf extract and/or purified steviol glycoside. The sweetener composition may be formulated into any edible sweetened substances. 1. A sweetener composition comprising:{'i': 'stevia', 'a leaf extract comprising at least 95% by weight of a mixture of steviol glycosides;'}a purified steviol glycoside composition comprising at least 95% by weight of a pure steviol glycoside; anda Luo Han Guo fruit extract comprising at least 40% by weight of Mogroside-V;wherein a weight ratio of a combination of the mixture of the steviol glycosides and the pure steviol glycoside to the Mogroside-V is in a range from 97:3 to 88:12.2stevia. The sweetener composition according to claim 1 , wherein the leaf extract claim 1 , the purified steviol glycoside composition claim 1 , and the Luo Han Guo Fruit extract have a natural origin.3. The sweetener composition according to claim 1 , wherein the mixture of steviol glycosides comprise Rebaudioside A in a range from 50% to 80% by weight.4. The sweetener composition according to claim 1 , wherein the pure steviol glycoside is one of Rebaudioside A claim 1 , Rebaudioside B claim 1 , Rebaudioside C claim 1 , Rebaudioside D claim 1 , Rebaudioside E claim 1 , Rebaudioside F claim 1 , Rebaudioside G claim 1 , Rebaudioside I claim 1 , Rebaudioside H claim 1 , Rebaudioside J claim 1 , Rebaudioside K claim 1 , Rebaudioside L claim 1 , Rebaudioside M claim 1 , Rebaudioside N claim 1 , Rebaudioside O claim 1 , Dulcoside A claim 1 , Stevioside claim 1 , and Rubusoside.5. The sweetener composition according to claim 1 , wherein the pure steviol glycoside is Rebaudioside-A.6. The ...

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Номер записи: 92
07-05-2015 дата публикации

METHOD FOR PRODUCING STEVIOL GLYCOSIDE

Номер: US20150128306A1
Автор: Ono Eiichiro
Принадлежит: SUNTORY HOLDINGS LIMITED

The invention provides a method for producing steviol glycosides. The invention provides a transformant having introduced therein the steviol glucosyltransferase and a method for producing steviol glycosides using the transformant. 2. The method according to claim 1 , wherein said sugar in the UDP-sugar is a hexose.3. The method according to claim 1 , wherein said sugar in the UDP-sugar is one selected from the group consisting of glucose claim 1 , mannose and galactose.4. The method according to claim 1 , wherein said sugar added to the carboxyl at position 19 is a hexose.5. The method according to claim 1 , wherein said sugar added to the carboxyl at position 19 is one selected from the group consisting of glucose claim 1 , mannose and galactose.6. The method according to claim 1 , wherein said Ris H or the sugar residue which is a glucose monomer claim 1 , a glucose dimer or a glucose trimer.7. The method according to claim 1 , wherein said compound is steviolmonoside claim 1 , steviolbioside or steviol.8. The method according to claim 1 , wherein said steviol glycoside is steviolbioside claim 1 , stevioside claim 1 , rebaudioside A claim 1 , rebaudioside B claim 1 , rebaudioside C claim 1 , rebaudioside D claim 1 , rebaudioside E claim 1 , rebaudioside F claim 1 , or a combination thereof.10. The transformant according to claim 9 , wherein said sugar molecule is a hexose.11. The transformant according to claim 9 , wherein said sugar molecule is one selected from the group consisting of glucose claim 9 , mannose and galactose.12. The transformant according to claim 9 , wherein said Ris H or the sugar residue which is a glucose monomer claim 9 , a glucose dimer or a glucose trimer.13. The transformant according to claim 9 , wherein said compound is steviolmonoside claim 9 , steviolbioside or steviol.14. The transformant according to claim 9 , wherein said polynucleotide is inserted into an expression vector.15. The transformant according to claim 9 , which is a ...

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Номер записи: 93
27-05-2021 дата публикации

PRODUCTION OF STEVIOL GLYCOSIDES IN RECOMBINANT HOSTS

Номер: US20210155966A1
Автор: Douchin Veronique, Nyffengger Christian, Olsson Kim, Sheridan Joseph Michael, Tatjer Recorda Laura
Принадлежит:

The invention relates to recombinant microorganisms and methods for producing steviol glycosides, glycosides of steviol precursors, and steviol glycoside precursors. 1. A method for producing one or more steviol glycosides and/or glycosylated steviol precursors , or a composition thereof , comprising whole cell bioconversion of a plant-derived or a synthetic steviol , steviol precursors , glycosylated steviol precursors , steviol glycosides , and/or a mixture thereof in a cell culture medium of a recombinant host cell expressing a gene encoding:(a) a polypeptide capable of glycosylating steviol or a steviol glycoside at its C-19 carboxyl group having at least 70% sequence identity to the amino acid sequence set forth in SEQ ID NO:4, and further having at least one amino acid substitution corresponding to residues 68, 69, 70, 71, 72, 73, 74, 75, 76, 77, 78, 79, 80, 81, 82, 83, 184, 260, 286, or 377 of SEQ ID NO:4;(b) a bifunctional polypeptide capable of glycosylating steviol or a steviol glycoside at its C-19 carboxyl group and glycosylating steviol or a steviol glycoside at its C-13 hydroxyl group, having at least 70% sequence identity to the amino acid sequence set forth in any one of SEQ ID NOs:132, 134, 136, 138, 140, 142, 144, 146, or 148; and/or(c) a tagged polypeptide capable of glycosylating steviol or a steviol glycoside at its C-19 carboxyl group, having at least 70% sequence identity to the amino acid sequence set forth in any one of SEQ ID NOs:174, 176, 178, or 180;wherein at least one of the polypeptides is a recombinant polypeptide; and producing the one or more steviol glycosides and/or the glycosylated steviol precursors, or the composition thereof, thereby.2. The method of claim 1 , wherein the one or more steviol glycosides and/or the glycosylated steviol precursors are claim 1 , or the composition thereof comprises steviol-13-O-glucoside (13-SMG) claim 1 , steviol-19-O-glucoside (19-SMG) claim 1 , steviol-1 claim 1 ,2-bioside claim 1 , steviol-1 ...

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Номер записи: 94
10-05-2018 дата публикации

BEVERAGE WITH SUPPRESSED DECREASE IN DEGREE OF SWEETNESS

Номер: US20180125109A1
Автор: IZUMI Akiko, NAGAO Koji, NISHIBORI Tomoyuki, URAI Soichiro
Принадлежит: SUNTORY HOLDINGS LIMITED

An embodiment of the present invention aims to provide a low-calorie natural beverage suppressed in decrease in sweetness during storage. Provided is a beverage meeting the following conditions: (A) a mass ratio of (RebD and/or RebM)/RebA is 0.52 to 1.58; and (B) a total Brix of RebA and RebD and/or RebM in terms of sucrose is 5 to 10. 1. A beverage meeting the following conditions:(A) a mass ratio of RebM/RebA is 0.52 to 1.58; and(B) a total Brix of RebA and RebM in terms of sucrose is 5 to 10.2. The beverage according to claim 1 , wherein the mass ratio is 0.52 to 1.05. An embodiment of the present invention relates to a beverage with suppressed decrease in degree of sweetness.There is an increasing demand for natural and low-calorie-oriented beverages. In pursuit of giving sweetness to beverages, much attention is being paid to high intensity natural sweeteners having a higher degree of sweetness than sucrose. In this context, a functional sweetener composition containing a vitamin, a high intensity sweetener, and a sweetness-improving composition is known (Patent Literature 1). There is also known a beverage containing a steviol glycoside as a natural sweetener (Patent Literature 2).Patent Literature 1: National Publication of International Patent Application No. 2009-517043Patent Literature 2: Japanese Patent Laid-Open No. 2015-502404Through research and development of natural and low-calorie-oriented beverages, the present inventors have found that a beverage containing a steviol glycoside as a high intensity sweetener experiences a decrease in sweetness during storage. A further study has led the inventors to infer that the decrease in sweetness is attributable to conversion of RebA which is a main component of a steviol glycoside.The present invention aims to provide a natural and low-calorie-oriented beverage with suppressed decrease in sweetness during storage.An embodiment of the present invention is a beverage meeting the following conditions: (A) a mass ...

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Номер записи: 95
11-05-2017 дата публикации

Consumables

Номер: US20170127702A1
Автор: HANSEN Chad Allen, SIMONS Christopher Todd, SLACK Jay Patrick
Принадлежит:

Disclosed are sweetened consumables and methods of forming said sweetened consumables that comprise certain sweeteners and at least one sweetness enhancer in a concentration near its sweetness detection threshold. The sweeteners include sucrose, fructose, glucose, high fructose corn syrup, corn syrup, xylose, arabinose, rhamnose, erythritol, xylitol, mannitol, sorbitol, inositol, acesulfame potassium, aspartame, neotame, sucralose, saccharine, or combinations thereof. The sweetness enhancer is selected from naringin dihydrochalcone, mogroside V, swingle extract, rubusoside, extract, rebaudioside, and stevioside. 1. A sweetened consumable comprising:a) at least 0.0001% of at least one sweetener comprising at least one of sucrose, fructose, glucose, high fructose corn syrup, corn syrup, xylose, arabinose, rhamnose, xylitol, erythritol, mannitol, sorbitol, inositol, acesulfame potassium, aspartame, neotame, sucralose, or saccharine,wherein said at least one sweetener is present in a concentration above the sweetness detection threshold in a concentration isosweet to 2% to 15% sucrose; andb) a sweetness enhancer comprising rubusoside and at least one of rebaudioside A or stevioside, wherein the sweetness enhancer is present in a concentration near its sweetness detection threshold, wherein for rubusoside this concentration is from 1.4 to 56 ppm, for rebaudioside A this concentration is from 1 to 30 ppm, and for stevioside this concentration is from 2 to 60 ppm,wherein the sweetness enhancer has a more than additive sweetness enhancing effect that is greater than the sweet taste due to the sweetness enhancer itself.2. The sweetened consumable of claim 1 , wherein said sweetness enhancer further comprises naringin dihydrochalcone.3. The sweetened consumable of claim 1 , wherein said sweetness enhancer further comprises neohesperidin dihydrochalcone.4. The sweetened consumable of claim 1 , wherein said sweetness enhancer further comprises naringin dihydrochalcone and ...

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Номер записи: 96
23-04-2020 дата публикации

STEVIA SWEETENER WITH IMPROVED SOLUBILITY

Номер: US20200120966A1
Автор: Shi Jingang, Wang Hansheng
Принадлежит:

The invention describes a stevia sweetener with improved solubility in an aqueous solution for convenience of use in the food and beverage industry. 122.-. (canceled)23. A method of improving the sweetness of a beverage , comprising:adding an effective amount of a steviol glycoside composition to the beverage,wherein the steviol glycoside composition is stable in the water or water/alcohol solution for at least 1 day to greater than 30 days at a temperature from 0° C. to about 25° C.,wherein the steviol glycoside composition comprises rebaudioside A and rebaudioside B, or rebaudioside A and rebaudioside D, or rebaudioside A and stevioside, or rebaudioside A, rebaudioside B and rebaudioside D, or rebaudioside A, rebaudioside B, rebaudioside D and stevioside, andwherein the steviol glycosides in the steviol glycoside composition has a solubility that is greater than or equal to 10 g/100 g water or water/alcohol solution.24. The method of claim 23 , wherein the steviol glycoside composition further comprises rebaudioside M.25. The method of claim 23 , wherein the steviol glycoside composition further comprises rebaudioside C.26. The method of claim 23 , wherein the steviol glycoside composition further comprises rebaudioside E.27. The method of claim 23 , wherein the steviol glycoside composition further comprises rebaudioside F.28. The method of claim 23 , wherein the steviol glycoside composition further comprises rubusoside.29. The method of claim 23 , wherein the steviol glycoside composition further comprises dulcoside A.30. The method of claim 23 , wherein the steviol glycoside composition further comprises a sweetener selected from the group consisting of cyclamates and salts thereof claim 23 , sucralose claim 23 , aspartame claim 23 , saccharin and salts thereof claim 23 , stevia claim 23 , rebaudioside A claim 23 , xylitol claim 23 , acesulfame-K claim 23 , neotame claim 23 , N [N-[3-(3-hydroxy-4-methoxyphenyl)propyl]-alpha-aspartyl]-L-phenylalanine-1-methyl ...

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Номер записи: 97
23-04-2020 дата публикации

Solubilized Steviol Glycoside Compositions

Номер: US20200120967A1
Автор: CHATURVEDULA VENKATA SAI PRAKASH, May James A., May Michael Perry, Zamora Julian E.
Принадлежит: JAMES AND CAROL MAY FAMILY, LLLP

Various embodiments of the present technology provide a sweetener composition comprising stevia glycosides that may be stably soluble in an aqueous solution, and methods for their manufacture. In various embodiments, the sweetener composition may comprise at least one stevia glycoside and a natural emulsifier. The natural emulsifier of the sweetener composition may enhance the aqueous solubility of the stevia glycoside to increase the concentration of dissolved stevia glycoside relative to its inherent aqueous solubility. The sweetener composition may be stably soluble upon formulation into any edible sweetened substances. 1. An aqueous sweetener solution comprising:a mixture of steviol glycosides in a range from 7% to 20% by weight;a Quillaja tree extract in a range from 0.1% to 2% by weight; andwater in a range from 88% to 93% by weight;wherein a weight ratio of the mixture of steviol glycosides to the Quillaja tree extract is in a range from 98:2 to 99.9:01.2. The aqueous sweetener solution according to claim 1 , wherein the aqueous sweetener solution is clear.3. The aqueous sweetener solution according to claim 2 , wherein the aqueous sweetener remains clear for at least six months at room temperature.4. The aqueous sweetener solution according to claim 2 , wherein the aqueous sweetener remains clear for at least two years at room temperature.5. The aqueous sweetener solution according to claim 1 , further comprising a natural liquid preservative in a range of 0.1% to 2% by weight.6. The aqueous sweetener solution according to claim 5 , wherein the natural liquid preservative is at least one of ascorbic acid claim 5 , tocopherol a herbal extract claim 5 , a fruit extract claim 5 , and combinations thereof.7. The aqueous sweetener solution according to claim 5 , wherein the aqueous sweetener solution is clear.8. The aqueous sweetener solution according to claim 1 , further comprising a preservative in a range of 0.1% to 2% by weight.9. The aqueous sweetener ...

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Номер записи: 98
23-04-2020 дата публикации

Solubilized Steviol Glycoside Compositions

Номер: US20200120968A1
Автор: CHATURVEDULA VENKATA SAI PRAKASH, May James A., May Michael Perry, Zamora Julian E.
Принадлежит: JAMES AND CAROL MAY FAMILY, LLLP

Various embodiments of the present technology provide a sweetener composition comprising stevia glycosides that may be stably soluble in an aqueous solution, and methods for their manufacture. In various embodiments, the sweetener composition may comprise at least one stevia glycoside and a natural emulsifier. The natural emulsifier of the sweetener composition may enhance the aqueous solubility of the stevia glycoside to increase the concentration of dissolved stevia glycoside relative to its inherent aqueous solubility. The sweetener composition may be stably soluble upon formulation into any edible sweetened substances. 1. An aqueous sweetener solution comprising:a mixture of steviol glycosides in a range from 7% to 20% by weight;a Quillaja tree extract in a range from 0.1% to 2% by weight; andwater in a range from 88% to 93% by weight;wherein the aqueous sweetener solution is clear.2. The aqueous sweetener solution according to claim 1 , wherein the aqueous sweetener remains clear for at least six months at room temperature.3. The aqueous sweetener solution according to claim 1 , wherein the aqueous sweetener remains clear for at least two years at room temperature.4. The aqueous sweetener solution according to claim 1 , wherein a weight ratio of the mixture of steviol glycosides to the Quillaja tree extract is in a range from 98:2 to 99.9:01.5. The aqueous sweetener solution according to claim 1 , further comprising a natural liquid preservative in a range of 0.1% to 2% by weight.6. The aqueous sweetener solution according to claim 5 , wherein the natural liquid preservative is at least one of ascorbic acid claim 5 , tocopherol a herbal extract claim 5 , a fruit extract claim 5 , and combinations thereof.7. The aqueous sweetener solution according to claim 5 , wherein the aqueous sweetener solution is clear.8. The aqueous sweetener solution according to claim 1 , further comprising a preservative in a range of 0.1% to 2% by weight.9. The aqueous sweetener ...

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Номер записи: 99
23-04-2020 дата публикации

STEVIA SWEETENER WITH IMPROVED SOLUBILITY

Номер: US20200120969A1
Автор: Shi Jingang, Wang Hansheng
Принадлежит:

The invention describes a sweetener with improved solubility in an aqueous solution for convenience of use in the food and beverage industry. 122.-. (canceled)23. A steviol glycoside composition with improved solubility in a water or water/alcohol solution ,wherein the steviol glycoside composition is stable in the water or water/alcohol solution for at least 1 day to greater than 30 days at a temperature from 0° C. to about 25° C.,wherein the steviol glycoside composition comprises rebaudioside A and rebaudioside B, or rebaudioside A and rebaudioside D, or rebaudioside A and stevioside, or rebaudioside A, rebaudioside B and rebaudioside D, or rebaudioside A, rebaudioside B, rebaudioside D and stevioside, andwherein the steviol glycoside composition has a solubility that is greater than or equal to 10 g/100 g water or water/alcohol solution.24. The steviol glycoside composition according to claim 23 , wherein the alcohol is selected from the group consisting of methanol claim 23 , ethanol claim 23 , propanol claim 23 , isopropanol claim 23 , n-butanol claim 23 , sec-butanol claim 23 , isobutanol claim 23 , tert-butanol claim 23 , n-pentanol claim 23 , sec-pentanol claim 23 , isopentanol and neopentanol.25. The steviol glycoside composition according to claim 23 , wherein the water or water/alcohol solution is from about 60% to about 1% alcohol by volume.26. The steviol glycoside composition according to claim 23 , wherein rebaudioside A is the main component.27. The steviol glycoside composition according to claim 23 , wherein the steviol glycoside composition is stable in water or water/alcohol solution at room or ambient temperature.28. A sweetener composition comprising the steviol glycoside composition according to and a sweetener.29stevia. The sweetener composition according to claim 28 , wherein the sweetener is selected from the group consisting of cyclamates and salts thereof claim 28 , sucralose claim 28 , aspartame claim 28 , saccharin and salts thereof ...

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Номер записи: 100