АППАРАТ ДЛЯ ОБРАБОТКИ ЖИДКОСТИ, СОДЕРЖАЩЕЙ ЦЕЛЕВОЕ ВЕЩЕСТВО

Группа изобретений относится к обработке жидкостей, содержащих целевое вещество, а именно жидкостей, содержащих биомолекулу, например, рекомбинантный полипептид. Раскрыты аппарат для обработки жидкости, аппарат для приготовления жидкой смеси, способ приготовления буферного раствора, способ получения биомолекулы. Аппарат для обработки жидкости включает один насос, расположенный ниже жидкостного соединения между соединением между исходным материалом и выпускным отверстием из регулятора расхода для сообщения движения потоку через регулятор расхода и из системы подачи жидкого исходного материала через устройство обработки посредством впускного отверстия устройства. Техническим результатом является упрощение конструкции аппарата для обработки жидкости и выполнение нескольких различных стадий обработки с использованием общей поточной линии. 4 н. и 7 з.п. ф-лы, 4 ил., 1 табл.

KONTINUIERLICHE CHROMATOGRAPHIE

Chromatographische Trennsaeule

DEVICE FOR SHUTTING-OFF AND CHANGING-OVER THE GAS AND LIQUID PASSAGES ESPECIALLY FOR GAS CHROMATOGRAPHS

... 1,243,435. Valves. CHEMOPBOJEKT. PROJEKTOVA INZENYRSKA A KONZULTAGNI ORGANISACE. 2 July, 1969 [5 July, 1968], No. 33415/69. Heading F2V. A valve for gas chromatographs comprises a body 1 in the wall of which is a groove 2, and a plurality of gas passages 7 opening into the groove, an elastic diaphragm 3 located on the wall of the body, a lid 4 located over the diaphragm, closing members 5 arranged in openings in the lid intermediate the passages, and a template 6 for actuating the closing members to shut-off or open the passages. In Fig. 2 (not shown), the closing members 5 are balls. The templates can be changed to give a different programme.

CHEMICAL ANALYTICAL APPARATUS

... 1366629 Gas chromatography C N GOODE C J THOMPSON and E W HORTON 29 Feb 1972 [1 Dec 1970] 56916/70 Heading B1H A chemical analytical instrument includes a pair of gas chromatographic columns, a mass spectrometer having an inlet port, piping interconnecting each gas chromatographic column individually to the said inlet port, a vent tube, piping interconnecting each gas chromatographic column individually to the vent tube and valve means whereby each gas chromatographic column may be selectively connected for throughflow of gas, either to the mass spectrometer or to the vent tube. While one column is being purged of the remains of a sample the other may be used for the next sample; more than two such columns may be so connected.

Gas phase partition chromatograph

... 1,067,241. Gas chromatography. INTERLECTRON S. A. April 8, 1964 [April 30, 1963], No. 14405/64. Heading B1L. A gas chromatograph includes at least one element having a gas channel whose width is substantially greater than its height, its width also increasing from inlet to outlet, so that a constant pressure is maintained in the channel. The element may be the sample injector, the column or the detector. Columns may be made from plates of Cu, Al, cupro-nickel alloys, stainless steel, light alloys, precious metals, glass, ceramics or plastics, by cutting rectilinear curvilinear, spiral or U-shaped grooves using mechanical, chemical, electrolytic, electrocrosion, ultrasonic or moulding techniques and then covering over the grooves with another plate. Thus a spiral-shaped capillary column of length 60-100M may be engraved in a plate 30cm x 30cm. If the plates are thin, the grooves can be made by drawing or stamping. Preferably the width to height ratio is 100 to 2:1. The grooves may be filled ...

Chromatographic process

... 1,135,522. Liquid chromatography. E. V. PIEL. 30 Aug., 1966 [15 Sept., 1965; 22 Dec., 1965], No. 38557/66. Heading B1X. In liquid column chromatography the chromatography bed consists of particles which have an average size of less than 1.5 microns, preferably less than 1 micron. The liquid mixture to be separated is caused to flow rapidly, preferably by centrifugal force, through the column and is separated by partition between the bed and a moving liquid solvent which is passed through the bed. In operating the centrifuge bucket apparatus of Fig. 1 a bed of particles is established by adding a slurry of particles and a solvent into tube 12 via reservoir 19, the bed being compacted by centrifuging the tube. A sample to be resolved is added to the top surface of the bed and the tube centrifuged thereby establishing chromatographic bands in bed 13 which can be identified visually or 'by elution. With the continuously operating apparatus of Figure 3 the particles e.g. a gel or thixotropic ...

Combined liquid chromatograph and mass spectrometer

An instrument combining a mass spectrometer with a liquid chromatograph has a low-pressure chamber that is evacuated by an evacuating equipment, an exit nozzle for allowing the effluent from the chromatograph to be ejected as a jet into the low-pressure chamber, and a connecting passage for conveying the jet of the effluent from the nozzle into the ionization chamber of the mass spectrometer, to stably supply the effluent from the chromatograph as the sample for the ion source into the ionization chamber. The instrument is further provided with a means for producing a flow of gas which moves along the inner walls of the low-pressure chamber and the connecting passage in such a way that the jet of the effluent is enveloped in the gas flow.

ANALYSIS

New chromatograph.

GASCHROMATOGRAPHIEANLAGE

CONTINUOUS CHROMATOGRAPHY

DEVICE FOR THE EVAPORATION OF SAMPLES, WHICH WERE INJECTED IN A COLUMN BY GAS CHROMATOGRAPHY.

DEVICE FOR BRANCHING OUT GAS FLOWS.

PROCEDURE FOR THE SEPARATION OF A CURRENT OF MEANS OF FINE CHANNELS AND PLANT FOR THE CRACKING OF FIXED PARTICLES.

ARRANGEMENT FOR COMBINATION OR ALLOCATION OF RIVERS WHEN CONNECTING CAPILLARIES INTO CHROMATOGRAPHI ANALYZERS.

MULTI-DIMENSIONAL ON-LINE CHROMATOGRAPHY SYSTEM WITH LARGE INJECTION CAPACITY FOR THE SUPERCRITICAL FLUID CHROMATOGRAPHY

Procedure and device for the technical execution of the Säulenchromatographie

PROCEDURE FOR THE SEPARATION FLUIDS OF A SUBSTANCE AND A DEVICE FOR IT

PROCEDURE AND DEVICE FOR SNAPPING THE SEPARATION AND/OR TRANSFORMATION OF SUBSTRATES

High performance liquid chromatography method and apparatus

Integrated protein affinity capture centrifugation device

Continuous electrolytically regenerated packed suppressor for ion chromatography

CHROMATOGRAPHIC CARTRIDGE AND HOLDER

PROTEIN CHROMATOGRAPHY SYSTEM

METHOD TO PERFORM LIMITED TWO DIMENSIONAL SEPARATION OF PROTEINS AND OTHER BIOLOGICALS

A method and apparatus are provided for performing capillary isoelectric focusing followed by mobilization of the focused zones by induced hydrodynamic flow or chemical mobilization. These two dimensions of separation are integrated with real-time whole-channel electrophoresis detection and automatic sample injection to achieve a separation resolution superior to that obtainable using known orthogonal capillary two dimensional arrangements.

METHOD AND APPARATUS TO PERFORM LIMITED TWO DIMENSIONAL SEPARATION OF PROTEINS AND OTHER BIOLOGICALS

A method and apparatus are provided for performing capillary isoelectric focusing followed by mobilization of the focused zones by induced hydrodynamic flow or chemical mobilization. These two dimensions of separation are integrated with real-time whole-channel electrophoresis detection and automatic sample injection to achieve a separation resolution superior to that obtainable using known orthogonal capillary two dimensional arrangements.

MODIFIED MEMBRANE SUPPRESSOR AND METHOD OF USE

Suppressor for suppressing the electrolyte of an eluent from a chromatographic column. The suppressor includes a regenerant compartment separated from an effluent compartment by an ion exchange membrane sheet permeable to ions of the same charge as its exchangable ions. Structure including ion exchange sites is provided in one or both compartments extending between the membrane sheet and compartment walls to define a continuous convoluted liquid flow through passage. Suitable structures are a screen or projections from the compartment walls. In a sandwich suppressor, two membrane sheets are included with an effluent flow channel between them. A detector is provided downstream of the suppressor.

PROCESS FOR ANALYTICAL DETERMINATIONS AND ROTOR INSERT ELEMENT THEREFOR

The present invention provides a process for carrying out analytical determinations by mixing and incubating a sample solution with at least one reagent solution and optical measurement of one parameter in the incubated reaction solution mixture, mixing, incubating and measuring being carried out during the action of a centrifugal force, wherein, under the influence of the centrifugal force, the sample solution is brought together with a soluble dry reagent, with at least partial dissolving thereof, and the mixture or the solution is forced centrifugally through a plurality of small hollow spaces, the centrifugal force and the flow resistance of the small hollow spaces being so adapted with regard to one another that a complete dissolving and mixing of the reaction components and possibly incubation takes place before the reaction solution passes from the small hollow spaces into a measuring chamber in which the measurement is carried out. The present invention also provides an insert element ...

PROCESS FOR ANALYTICAL DETERMINATIONS AND ROTOR INSERT ELEMENT THEREFOR

METHOD OF MANUFACTURING A SEPARATING COLUMN OF A VITREOUS MATERIAL FOR USE IN AN APPARATUS FOR GAS CHROMATOGRAPHY

SEQUENTIAL DETECTION ION CHROMATOGRAPHY

Packings or equivalent structures which produce radial mixing in channels can be used to enhance reagent addition devices for ion chromatography post column reactions in which band spreading at detrimental levels is effectively controlled. Application to two dimensional conductometric detection in simultaneous suppressed and nonsuppressed ion chromatography.

FLUIDIC DEVICES AND METHODS OF USING THEM

Certain embodiments described herein are directed to chromatography systems that include a microfluidic device configured to provide three-way switching or switching between three or more inputs or outputs. The microfluidic device can be fluidically coupled to one or more switching valves to provide for selective control of fluid flow in the chromatography system.

Verfahren zur kontinuierlichen, chromatographischen Trennung von Mehrstoffgemischen

Verfahren und Vorrichtung zur Abtrennung wenigstens eines Stoffes aus einer Lösung durch Adsorption bzw. Absorption.

Installation de chromatographie

Procédé pour la séparation chromatographique d'un fluide, appareil pour la mise en oeuvre de ce procédé et application de ce procédé

Anordnung mit mindestens einer chromatographischen Röhre

Verfahren und Vorrichtung zur gaschromatographischen Trennung von Mischungen

Verfahren zur Herstellung einer Trennsäule aus einem glasartigen Werkstoff, insbesondere für eine gaschromatographische Vorrichtung

Procédé de séparation des composants d'un échantillon liquide

Probeteiler für Gasanalysatoren

Verbindungsstück zum Verbinden einer chromatografischen Säule, die einen grossen Querschnitt besitzt, mit der Zuführleitung für die Pufferflüssigkeit, die einen kleinen Querschnitt besitzt

Colonne de chromatographie

Colonne pour la chromatographie en phase vapeur

Verfahren zur gaschromatographischen Trennung

Verfahren und Einrichtung zur kontinuierlichen Durchführung der Flüssig-fest-Chromatographie

INSTALLATION D'ANALYSE EN CONTINU PAR CHROMATOGRAPHIE SUR FILM MINCE.

Verfahren zur Herstellung von Kapillarsäulen für die Gaschromatographie

Procedimento e dispositivo per cromatografia gassosa ridimensionale a scopo preparativo

Dispositif de chromatographie de partition en phase gazeuse

Procédé pour la séparation de substances contenues dans un mélange de composés chimiques et colonne chromatographie gaz-solide par la mise en oeuvre du procédé

Verfahren und Vorrichtung zur technischen Durchführung der Säulenchromatographie

Appareil pour réaliser une interaction physique et chimique de substances fluides au voisinage immédiat de surfaces solides

Gaschromatographische Messeinrichtung, die aus Trägergasquelle, Probendosiereinrichtung, Trennsäule und Detektor besteht

Verfahren und Vorrichtung zur Anzeige bzw. Messung mindestens eines oxydierbaren bzw. reduzierbaren Bestandteils eines Eluats auf elektrochemischem Weg

Porous synthetic resin structure

Apparatus for division-separation operation has a porous filling with a three dimensional pore structure, which serves to pass at least one phase, the adjoining faces of the structure serving as support for at least one other phase. The porous filling is characterised by a combination of (a) a large number of the connected hollow pores are enclosed by solid parts, which also have a three dimensional cohesive structure; (b) the hollow pores are interrupted in all cross-sections of the filling, in a direction perpendicular to the direction of flow, by solid particles, which are also mutually connected; and (c) the porosity characteristics of the filling as a whole are essentially homogeneous, at least perpendicular to the direction of flow.

Verfahren zur Übertragung von in einem Gasstrom enthaltenen Stoffen in einem Flüssigkeitsstrom und Vorrichtung zur Ausführung des Verfahrens

Procédé de chromatographie gazeuse et appareil pour sa mise en oeuvre

CENTRIFUGAL CHROMATOGRAPHY MECHANISM.

DEVICE FOR CONSOLIDATING THE FUELLSTOFFES OF A CHROMATOGRAPHY CARTRIDGE.

Reagent for detecting antigen-antibody reactions

A solvent-based mixt. for chromatographic analyses is applied to the centre of a circular disc (4) of adsorbent material resting on a support plate (15) in a rotor (3) driven by a central shaft (10) within an enclosed chamber. Centrifugal material is intercepted by the inner wall of a collector ring (5) round the support plate and rotated by the same shaft. The inner wall of the collector ring is inclined, and each point on its inner wall is farther from the rotation axis (14) than the most distant point of the adsorbent disc. At two or more (even numbered) points in the slightly irregular collector ring, at max. radial distance from the centre, are discharge bores (6) taking solvent towards an axial outlet, e.g. for re-use.

Chromatograph and extraction system

In a liquid/liquid chromatography or extraction p4rocess the mobile phase (2) is injected in the column with the stationary phase (1) at such a speed (40/80 cm/sec.) that at the entry (3) a continuous stream (13) is formed which disintegrates into droplets (14). This permits larger tubes (5) to be used. Their inner diameter B should have a ratio to the length C of 1:30 to 1:70. The entry diameter D should be less than B/2.

Control valve for the selective switching of a sample fluid between a measuring path and an outlet path or between a first and a second measuring path

The control valve is designed for switching a sample fluid between an analyser and an outlet path (13). It has a housing (1) which can be attached sealingly onto a sample-fluid feed conduit (3) and which is provided, for receiving the sample-fluid feed conduit (3), with a bore (2) into which one end of a capillary (8) projects and, furthermore, a first control-fluid conduit (12) opens, and from which a discharge channel (13) extends. The capillary (8) is accommodated concentrically in the passage bore (7) in a lining tube (6) connected to the valve housing (1) and possesses, in the vicinity of its introduction into the housing bore (2), a wall perforation (15) for introducing control fluid into the capillary bore via a second control-fluid conduit (14). By applying an approximately equal control-fluid pressure both to the housing bore (2) and to the capillary bore, with the discharge channel (13) closed, a direct throughflow of the sample fluid from the feed conduit (3) to the outflow end ...

Reagent for detecting antigen-antibody reactions

A solvent-based mixt. for chromatographic analyses is applied to the centre of a circular disc (4) of adsorbent material resting on a support plate (15) in a rotor (3) driven by a central shaft (10) within an enclosed chamber. Centrifugal material is intercepted by the inner wall of a collector ring (5) round the support plate and rotated by the same shaft. The inner wall of the collector ring is inclined, and each point on its inner wall is farther from the rotation axis (14) than the most distant point of the adsorbent disc. At two or more (even numbered) points in the slightly irregular collector ring, at max. radial distance from the centre, are discharge bores (6) taking solvent towards an axial outlet, e.g. for re-use.

CONTINUOUS FLOW ANALYSIS SYSTEM, IN PARTICULAR FLOW INJECTION ANALYSIS SYSTEM.

Method for synthesizing metal chelated affinity membrane chromatographic medium for radial chromatographic column

A process for synthesizing chromatographic medium of metal chelated affinity membrane for radial chromatographic column includes such steps as autopolymerization of epoxy propyl methylacrylate, graftreaction on cotton fibre, ring-opening reaction on imido-acetic acid, and chelating reaction on transition metal ions, and features easily available raw materials, simple reaction conditions, easy control and high binding capacity. The radial chromatographic column filled with said medium can be used to separate and purify biological macromoleculae (human serum albumin, catalase or urokinase) with high treated volume, flow speed and purity, and linear amplification.

CHROMATOGRAPHY DEVICE

DOUBLE FLOW CHROMATOGRAPHY SYSTEM

The disclosed invention relates to a new liquid chromatographic method, wherein the flow direction of sample is directed alternately in an essentially horizontal direction and in an essentially vertical direction through a separating matrix having a dimensional ratio between the column width and column height of at least 1:0.5, preferably at least 1:0.2, more preferably at least 1:0.1 , even more preferably at least 1:0.04. The invention further relate to an apparatus and a column design for controlling the flow direction of a liquid sample through a separating matrix in either an essentially horizontal or in an essentially vertical direction.

METHOD AND APPARATUS FOR THE ISOTOPE-RATIO ANALYSIS

The invention relates to a method and to an apparatus for the isotope-ratio analysis. The method comprises the following steps: - carrying out an LC method and thus providing a first eluate which contains at least one first liquid carrier fluid and one or more analytes, - collecting a portion of interest of the eluate, - preparing the partial eluate of interest by combining with a second liquid carrier fluid and separating out the first carrier fluid to form a prepared partial eluate, - preparing the prepared partial eluate to form one or more gaseous transformation products of the analytes, - supplying the gaseous transformation products with gaseous carrier fluid to an isotope analyzer and determining the isotope ratios.

DETECTION METHOD AND APPARATUS FOR CHROMATOGRAPHY

Liquid chromatograph

A liquid chromatograph comprises an analyzing chip in which a capillary is formed in a substrate and a detector section is disposed downstream of the capillary. The capillary is covered so that an inlet opening end and an outlet opening end of said capillary flow path is opened. A frame member has a sample introduction path, a carrier liquid introduction path and a liquid discharge path. The analyzing chip is movably disposed in the frame member so that the inlet opening end and the outlet opening end of the capillary are selectively in communication with and/or under interruption against said sample introduction path, the carrier liquid introduction path and the liquid discharge path. The liquid chromatograph is very small in size but is easy to handle. Incidentally, there is disclosed also a sample introduction apparatus and method for the chromatograph, and an FET type detector suitable for the chromatograph.

Micromechanical ejection pump for separating small fluid volumes from a flowing sample fluid

A micromechanical ejection pump for extracting small fluid volumes from a flowing sample fluid is provided with a substrate, and a canal for pressurized working fluid. The micromechanical ejection pump also has a canal for ejecting a fluid volume from the sample fluid. The working fluid canal runs into the extension of the sample fluid canal discharging crosswise thereto. The ejection canal connected to the sample fluid canal branches off from the working fluid canal opposite to the sample fluid canal. The working fluid canal and the ejection canal have in particular a substantially similar cross-section of 0.01-0.12 mm2, wherein the ratio of the cross-section of the sample fluid canal to the cross-section of the working fluid canal or the ejection canal is preferably between 0.2 and 0.5, more particularly between 0.3 and 0.4 and most preferably 0.35.

Low-retention pre-columns: a straightforward approach to enable larger injection volumes and reduce extra-column effects in HPLC

Low-retention pre-columns that allow increased injection volumes of solvents chromatographically stronger than the mobile phase and use of solvents with limited solubility in the mobile phase, such as ethyl acetate and MTBE. The system and method also reduces band broadening due to the extra-column effects acting upstream of the analytical column, including band broadening due to the injection process and due to the connecting tubing and fittings between the injection system and the column. A pre-column may also be used as a guard column, thereby minimizing band broadening due to the guard column.

Coupled Analytical Instruments for Dual Mode FTIR/GC-FTIR

A system and method are disclosed for analyzing samples, which includes a spectrometry system for detecting components of a sample; a gas chromatography column for separating the components of a sample; a first sample unit for receiving a first sample from a sample source; and a second sample unit for receiving a second sample from a sample source. Each sample loop unit allows independent processing of samples in preparation for analysis.

Partial modulation via pulsed flow modulator for comprehensive two-dimensional liquid or gas chromatography

This invention is a method and device for use with multi-dimensional chromatography that utilizes partial modulation. An analyte-bearing sample is subjected to a first dimension of chromatography. Thereafter the separated analyte-bearing sample is diluted with a modulated second carrier such at the analyte-bearing sample is not stopped or its temperature altered. The partially modulated analyte-bearing sample then feeds into a secondary column where the analyte-bearing sample is further separated.

APPARATUS FOR CONTINUOUS CHROMATOGRAPHIC SEPARATIONS

CHROMATOGRAPHY APPARATUS

Liquid chromatograph including passage switch valve

A liquid chromatograph includes: an analysis column; a sample loop that temporarily contains a mixed liquid of a sample and a mixture solvent; and a passage switch valve capable of switching a passage between a load position where the mixed liquid is temporarily held in the sample loop and an injection position where the mixed liquid held in the sample loop is sent to the analysis column. In the liquid chromatograph, a specific passage is formed in each of the load position and the injection position.

ANALYTE CONCENTRATOR SYSTEM AND METHODS OF USE

Systems and methods for concentrating an analyte preparatory to analysis thereof include processing the effluent of an analyte concentrator to produce an eluent for eluting an analyte retained in the same or separate concentrator, and systems implementing the same. The analyte concentrator system connects the effluent outlet of an analyte concentrator column to an eluent generation module such that the substantially analyte-free effluent discharged from the analyte concentrator column passes fluidly into the eluent generation module. Eluent generated from the substantially analyte-free effluent in the eluent generation module is likewise substantially free of the analyte. The systems and methods can minimize and/or (substantially) eliminate background signal during analysis of the concentrated analyte.

CONTINUOUS AND SEPARATING GAS ANALYSIS

ANALYSIS OF TRACE ION SPECIES AND ANALYZING APPARATUS USING METHOD THEREOF

PURPOSE: To effect efficient analysis at a trace ion species, by allowing the same column, to perform concentration of the required trace ion species in a specimen liquid for measurement and chromatographic of this ion species. CONSTITUTION: A specimen liquid for measurement in a bath 1b flows through a path comprising pump 2B → selector valve 5 → 2nd. column 4b → selector valve 5' → bath 1e. Thus,on the upper reach side of the 2nd column 4b, the ion species in the specimen liquid is caught and accumulated and a trace of the required ion species is concentrated. When the selector valves 5, 5' are operated for switching of the specified time after starting of admission of the specimen liquid into the 2nd column 4b, the ion species accumulated on the upper reach side of the column 4b is separated from the upper reach side of the column 4b by an elutant from the bath 1a and chromatogaphic separation is performed on the lower reach of the column 4b. Thus, as concentration and separation are ...

SAMPLE MODULATING CELL FOR GAS CHROMATOGRAPHY

SAMPLE ADJUSTING DEVICE AND SAMPLE ADJUSTING DEVICE FORMING METHOD

PROBLEM TO BE SOLVED: To provide a sample adjusting device and a method for removing impurities and undesired substance from a sample, ahead of mass spectrometry by matrix auxiliary laser desorption/ionization (MALDI), electro-spray ionization(ESI) or the like. SOLUTION: A substrate used in this sample adjusting device can be formed in a probe 50 shape or as a standard pipette chip. A chromatography medium is attached to the inner surface of the chip in order to form a single layer of particles easily contactable to a fluid flowing the pipette chip. The device can be used for washing, protein coupling, elution treatment or the like. COPYRIGHT: (C)2001,JPO ...

СПОСОБ ХРОМАТОГРАФИЧЕСКОГО РАЗДЕЛЕНИЯ СМЕСИ КОМПОНЕНТОВ

Изобретение может быть использовано в химической, гидрометаллургической, микробиологической, фармацевтической отраслях промышленности. Хроматографическую колонку 1 заполняют первой фазой, которую удерживают в ней. Вторую фазу прокачивают через колонку 1, подавая ее в дискретном режиме с периодически изменяющейся скоростью отдельными порциями во входное сечение 4. Дискретный режим осуществляют с помощью клапана 5 и автоматического регулятора 6, включенных в линию подачи второй фазы 3. Затем в хроматографическую колонку 1 по линии 8 вводят в виде импульса смесь компонентов. После многократного нестационарного распределения компонентов между первой и второй фазами обогащенные фракции отдельных компонентов выводят из колонки 1 со второй фазой по линии 9. Можно использовать спиралевидный канал хроматографической колонки 1, например, в виде трубки, намотанной на поверхность цилиндра; или канал в виде ряда последовательно соединенных камер. Первую фазу удерживают в хроматографической колонке 1 ...

Способ определения суммы сесквитерпеновых кислот в пересчете на валереновую кислоту методом ВЭЖХ в лекарственных средствах

Изобретение относится к способу количественного определения суммы сесквитерпеновых кислот в лекарственном препарате «Валерианы настойка» в лекарственной форме «настойки», или в лекарственном препарате «Валерианы экстракт» в лекарственной форме «таблетки, покрытые пленочной оболочкой», или в фармацевтической субстанции «Валерианы экстракт густой» методом высокоэффективной жидкостной хроматографии (ВЭЖХ), включающему использование спирта этилового 50 % в качестве растворителя для приготовления растворов испытуемого образца лекарственного препарата «Валерианы экстракт» в лекарственной форме «таблетки, покрытые пленочной оболочкой», и фармацевтической субстанции «Валерианы экстракт густой», колонки для ВЭЖХ с октадецилсилилсиликагельным сорбентом, градиентный способ элюирования с двумя подвижными фазами: ацетонитрилом и фосфорной кислоты концентрированной раствором 5,0 г/л в воде, спектрофотометрическое детектирование при длине волны 220 нм, использование валереновой кислоты в качестве стандартного ...

СИСТЕМА ВЫСОКОЭФФЕКТИВНОГО ЖИДКОСТНОГО ХРОМАТОГРАФИЧЕСКОГО АНАЛИЗА С ПОТОКОВЫМ ДЕТЕКТОРОМ

Система высокоэффективного жидкостного хроматографического анализа с потоковым детектором, содержащая насос, датчик расхода с выходным сигналом UA, устройство ввода пробы, термостатируемую высокоэффективную хроматографическую колонку, потоковый хроматографический детектор с выходным сигналом UD, устройство регистрации выходного сигнала системы анализа и имеющий входной сигнал UC интерфейс связи детектора с устройством регистрации выходного сигнала системы анализа, отличающаяся тем, что между выходом детектора и входом интерфейса связи установлено устройство нормировки выходного сигнала детектора с двумя входами, один из которых связан с выходом детектора, а другой связан с выходом датчика расхода, и с одним выходом, связанным с входом интерфейса связи, при этом входной сигнал интерфейса связи UC связан с выходным сигналом детектора UD и выходным сигналом датчика расхода UA равенством UC=UD/UA.

Газовый хроматограф

Изобретение относится к аналитическому приборостроению, а именно к хроматографии, и найдет применение в нефтеперерабатывающей и нефтехимической отраслях промышленности для контроля количественного состава продуктов технологических установок. Цель изобретения - повышение точности и чувствительности. Газовый хроматограф содержит две испарительные камеры, снабженные нагревателями, из которых одна соединена с разделительной колонкой, а другая - с размывателями. Выходы разделительной колонки и размывателя соединены с детектором. 1 ил.

Verfahren zur massenspektrometrischen Untersuchung gasförmiger Komponenten

MIKROHERGESTELLTER CHEMISCHER SENSOR AUF DIFFUSIONSBASIS

Chromatographisches Trennverfahren und Vorrichtungen zur Durchfuehrung des Verfahrens

Verfahren zur Herstellung einer Trennsaeule aus glasartigem Material fuer eine gaschromatographische Vorrichtung

Verfahren zur selektiven Abtrennung nach einem chromatographischen Verfahren

Device for taking samples from sample vessels and placing in analysis unit comprises heating arrangement for sample vessels, and units for impinging sample vessels with carrier gas

A device for taking samples from sample vessels (13) and placing in an analysis unit (23) comprises a heating arrangement (15) for the sample vessels, and units for impinging the sample vessels with a carrier gas and consisting of a line (11, 16) for connecting to the sample vessel on one side via a first restriction (12) and for connecting to a sample retaining unit (17) on the other side to which is connected a stop valve (19) on one side and the analysis unit on the other side via a second restriction (20).

Instrument zur Messung organischer Nichtmethangase in Gasproben

Fluid Switching Valve

A fluid switching valve includes a first valve element (e.g., a stator) that has a plurality of first fluid-conveying features (e.g., ports), and a second valve element (e.g., a rotor) that has one or more second fluid-conveying features (e.g., fluid conduits in the form of grooves). The second valve element is movable, relative to the first valve element, between a plurality of discrete positions such that, in each of the discrete positions, at least one of the one or more second fluid-conveying features overlaps with multiple ones of the first fluid conveying features to provide for fluid communication therebetween. At least one of the first valve element and the second valve element includes a recess. The recess serves to reduce wear between the first valve element and the second valve element. The recess is arranged such that it does not overlap with any of the first fluid-conveying features or any of the second fluid-conveying features when the rotor is in any of the discrete positions. 1. A fluid switching valve comprising:a first valve element having a plurality of first fluid-conveying features;a second valve element having one or more second fluid-conveying features,wherein the second valve element is movable, relative to the first valve element, between a plurality of discrete positions such that, in each of the discrete positions, at least one of the one or more second fluid-conveying features overlaps with multiple ones of the first fluid conveying features to provide for fluid communication therebetween, andwherein at least one of the first valve element and the second valve element includes a recess, the recess serving to reduce wear between the first valve element and the second valve element and arranged such that it does not overlap with any of the first fluid-conveying features or any of the second fluid-conveying features when the rotor is in any of the discrete positions.2. The fluid switching valve of claim 1 , wherein the recess is positioned ...

FLUID INTERFACE BETWEEN FLUID LINES OF DIFFERING CROSS-SECTIONAL AREA

A fluid transfer device transfers a fluid from a first fluid channel with a first cross-sectional area into a second fluid channel with a second cross-sectional area, larger than the first cross-sectional area. The fluid transfer device includes a fluid inlet interface at which the fluid is transferable from the first fluid channel into the fluid transfer device; an inlet branch configured to split the fluid from the first fluid channel into multiple inlet branch channels; multiple outlet branches, each of which is configured to split the fluid from the inlet branch channels into respective outlet branch channels; and a fluid outlet interface configured to transfer the fluid in the outlet branch channels into the second fluid channel. The inlet and output branches and branch channels are disposed such that the fluid exits from the fluid outlet interface, distributed in a two-dimensional manner across the second cross-sectional area. 1. A fluid transfer device for transferring a fluid from a first fluid channel with a first cross-sectional area at an outlet side into a second fluid channel with a second cross-sectional area at an inlet side , the second cross-sectional area being larger than the first cross-sectional area , the fluid transfer device comprising:a fluid inlet interface at which the fluid is transferable out of the first fluid channel into the fluid transfer device;an inlet branch fluidically coupled with the fluid inlet interface, and configured to split the fluid from the first fluid channel into a plurality of inlet branch channels;a plurality of outlet branches fluidically coupled with the inlet branch channels, wherein each of the outlet branches is configured to split the fluid from the inlet branch channels into a respective plurality of outlet branch channels; anda fluid outlet interface fluidically coupled with the outlet branch channels, and configured to transfer the fluid in the outlet branch channels into the second fluid channel,wherein ...

Continuous Backpressure Monitoring to Determine the System Health State of HPLC Systems

A method for determining a state of a fluidic system can include measuring back pressures in the fluidic system at different times and determining a state of the fluidic system. The determination is based on at least the measured back pressures and on additional status information indicative of at least one status of the fluidic system at at least one of the different times. 1. A method for determining a state of a fluidic system , the method comprising:measuring back pressures in the fluidic system at different times; anddetermining the state of the fluidic system, wherein the determining the state is based on at least the measured back pressures.2. The method according to claim 1 , wherein the determining the state is also based on an additional status information at at least one of the different times claim 1 , andwherein the determining the state of the fluidic system comprises: comparing the measured back pressures to at least one reference.3. The method according to claim 2 , wherein the at least one reference is based on the back pressures measured under conditions which are at least comparable to conditions at the different times.4. The method according to claim 2 , wherein the reference used in the comparing the measured back pressure depends on the additional status information.5. The method according to claim 1 , wherein the determining the state of the fluidic system further comprises:determining a deviation when an absolute value between one of the measured back pressure and at least one reference exceeds a threshold.6. The method according to claim 5 , wherein the fluidic system comprises a pump configured to generate a liquid flow claim 5 ,wherein the fluidic system is configured to include different configurations,wherein the configurations differ at least as regards which components are fluidly connected to the pump; 'the fluidic system assuming a second configuration, which is different from the first configuration, and determining whether the ...

LIQUID CHROMATOGRAPHY SYSTEMS

A liquid chromatographic (LC) system is introduced which comprises at least one fluidic stream, the fluidic stream comprising a sample-injection valve, a trap-bypass-selection valve, a column-bypass valve, a load-elute valve and a trap-selection valve. Also, a liquid chromatographic (LC) system is introduced which comprises at least one fluidic stream. The fluidic stream comprises a first substream and a second substream. The first substream comprises a first sample-injection valve, a load-elute valve and a trap-selection valve. The second substream comprises a second sample-injection valve and a column-bypass valve. The fluidic stream further comprises a trap-LC substream transfer valve and a substream-selection valve. The LC systems provide a broad choice of chromatographic options and modes and enable to flexibly and rapidly switch between them. 1. A liquid chromatographic (LC) system comprising at least one fluidic stream , the fluidic stream comprising:a sample-injection valve comprising a plurality of ports and a multi-way switch to switch fluidic connections between ports, including a sample input port fluidically connected to a sample input nozzle, an aspiration/dispensing-pump port fluidically connected to a sample aspiration pump, a sample-loop-input port and a sample-loop-output port interconnected by a sample loop, an LC-pump port fluidically connected to an LC pump and a sample-injection-to-trap-bypass-selection port;a trap-bypass-selection valve comprising a plurality of ports and a multi-way switch to switch fluidic connections between ports, including a trap-bypass-selection-to-sample-injection port fluidically connected to the sample-injection-to-trap-bypass-selection port of the sample injection valve, two bypass ports interconnected by a bypass fluidic path, a trap-bypass-selection-to-column-bypass port and two trap-bypass-selection-to-load-elute ports;a column-bypass valve comprising a plurality of ports and a multi-way switch to switch fluidic ...

MODULAR AUTOMATED CHROMATOGRAPHY SYSTEM

Valves, pumps, detectors, sample loops, fraction collectors and the like are individually incorporated into modules that are mountable at individual mounting sites on a base unit which also supports one or more chromatography columns. Each module includes fluid connections to other modules and a microcontroller joining the module to a computed and monitor through an electronic connector at each mounting site. The fluid connections between the modules and the column(s) are removed from the electronic connections and accessible to the user. A software platform may recognize the modules and their locations, coordinate fluid connections between the modules, and provide a variety of control, monitoring, data generating and data processing functions to generate chromatographic data. The software platform may also provide graphical tools for designing chromatographic methods from a library of phases. 1. A system for joining a plurality of fluid manipulation components into a flow scheme for directing fluids to and from a chromatographic separation device and for operating said joined fluid manipulation components according to a selected protocol , said system comprising:a plurality of modules, each said module comprising (a) one of said fluid manipulation components, and (b) a microcontroller that transmits operational signals to said fluid manipulation component in response to commands received from outside said module;a mounting frame comprising a plurality of bays, each of the plurality of bays constructed to receive a single module, and each of the plurality of bays comprising a signal connector that couples to and communicates with the microcontroller of a module mounted in said bay, wherein at least some of the bays are constructed to receive single modules interchangeably with other said modules; anda software platform in communication with the microcontroller of each module, said software being programmed to (1) receive from each of the plurality of modules ...

MODULAR AUTOMATED CHROMATOGRAPHY SYSTEM

Valves, pumps, detectors, sample loops, fraction collectors and the like are individually incorporated into modules that are mountable at individual mounting sites on a base unit which also supports one or more chromatography columns. Each module includes fluid connections to other modules and a microcontroller joining the module to a computed and monitor through an electronic connector at each mounting site. The fluid connections between the modules and the column(s) are removed from the electronic connections and accessible to the user. A software platform may recognize the modules and their locations, coordinate fluid connections between the modules, and provide a variety of control, monitoring, data generating and data processing functions to generate chromatographic data. The software platform may also provide graphical tools for designing chromatographic methods from a library of phases. 1. A system for configuring and operating a plurality of fluid manipulation components joined into a flow scheme for directing fluids to and from a chromatographic separation device , the system comprising:a plurality of modules, each of the plurality of modules comprising (a) one of the fluid manipulation components, and (b) a microcontroller that transmits operational signals to the fluid manipulation component in response to commands received from outside the module;a mounting frame comprising a plurality of bays, each of the plurality of bays constructed to receive a single module, and each of the plurality of bays comprising a signal connector that couples to and communicates with the microcontroller of a module mounted in the bay, wherein at least some of the bays are constructed to receive single modules interchangeably with other modules; anda software platform in communication with the microcontroller of each module, the software platform having a user interface configured to:display a library of descriptions of phases usable in a chromatography process; andenable a ...

CARRIER GAS CONNECTION DEVICE FOR GAS CHROMATOGRAPHS

Carrier gas connection devices () enable one to change the carrier gas provided to a gas chromatography channel. The carrier gas connection devices () comprise a channel adaptor (), a carrier block (), and a clamping system, and two or more channel adaptor positions. In each of the channel adaptor positions, a fluid-tight flow path for carrier gas out of the carrier block () into the channel adaptor () is formed. Carrier gas distribution systems (), gas chromatography instruments, and methods of changing a carrier gas supplied to a GC are also provided. 1. A carrier gas connection device comprising:a carrier block comprising first and second carrier block carrier gas passageways, and each of the carrier block carrier gas passageways comprises an entrance and exit;a channel adaptor comprising a channel adaptor carrier gas passageway having an entrance and an exit;a clamping system that fastens the carrier block to the channel adaptor in a substantially fluid-tight connection; andthe carrier gas connection device comprises first and second channel adaptor positions wherein:(i) in the first channel adaptor position, the channel adaptor carrier gas passageway entrance aligns with the first carrier block carrier gas passageway exit to form a fluid-tight flow path for a first carrier gas out of the carrier block into the channel adaptor, and(ii) in the second channel adaptor position, the channel adaptor carrier gas passageway entrance aligns with the second carrier block carrier gas passageway exit to form a fluid-tight flow path for a second carrier gas out of the carrier block into the channel adaptor.2. The carrier gas connection device of claim 1 , wherein the channel adaptor is configured for switching from one of the channel adaptor positions to another of the channel adaptor positions by manual movement.3. The carrier gas connection device of claim 1 , wherein the channel adaptor comprises one or more sealing surfaces configured to form a fluid-tight plug of one ...

Apparatus for Field-Flow Fractionation

The present invention relates to an apparatus for field-flow fractionation and to a method for separating samples by means of field-flow fractionation using this apparatus. The apparatuses of the invention comprise one or more reservoirs, a pump, a first flow volume splitting device, two valves for flow control, an injector, a separation channel having a first connector at a first end and a second connector at a second end or an AF4 separation channel having a first connector at a first end, a second connector at a second end and a third connector between the first and the second ends, optionally a second flow volume splitting device, a back-pressure element, one or more detector(s), a flow volume control device and one or more waste container(s). The apparatuses of the invention are characterized in that they comprise a valve for flow control in a first flow path and a second flow path which connect the pump to the first or the second or the third connector of the separation channel and that these valves are switched alternately in a controllable time ratio. 1. An apparatus for field-flow fractionation , comprising one or more reservoirs , a pump , a first flow volume splitting device , a first valve , an injector , a separation channel with a first connector at a first end and a second connector at a second end , a second flow volume splitting device , a second valve , a back-pressure element , one or more detectors , a flow volume control device and one or more waste containers , whereinthe first flow volume splitting device is suitable to split the volume flow generated by the pump into a first flow path connecting the pump to the first connector of the separation channel and a second flow path connecting the pump to the second connector of the separation channel,the injector is arranged in the first flow path,the second flow volume splitting device is arranged within the second flow path and is suitable to split the volume flow of the second flow path between ...

SPIRAL WOUND PROTEIN SEPARATION DEVICE

The present invention is directed to an affinity chromatography device that has a normal flow and which separates a targeted protein from aqueous mixtures. The chromatography device includes a housing containing therein a spiral wound membrane assembly that includes at least one inner intermediate material that forms an outer flow channel, at least one polymer membrane that contains therein inorganic particles, and at least one outer intermediate material that forms an inner flow channel sequentially positioned around a central core having a solid outer wall. An aqueous mixture is passed through the outer flow channel, through the polymer membrane where the targeted protein is removed, and then through an inner flow channel. The affinity chromatography device further includes an inlet flow distributor containing an inlet and an outlet flow distributer containing an outlet. Additionally, the chromatography device has a dimensionless resistance parameter that is less than 0.08. 1. An affinity chromatography device comprising:an exterior housing;an inlet flow distributor having therein an inlet to permit fluid flow into said housing and being located at a first end of said housing;a centrally located core; and [ 'wherein said at least one inner intermediate material includes an inner flow channel,', 'at least one inner intermediate material,'}, 'a polymer membrane having therein inorganic particles having a nominal particle size; and', wherein said at least one outer intermediate material includes an outer flow channel,', 'wherein said inner intermediate material, said polymer membrane, and, 'at least one outer intermediate material,'}, 'said outer intermediate material are positioned around said core; and, 'a wound membrane assembly disposed within said housing, said wound membrane assembly comprisinga flow distributor positioned at an opposing second end of said wound membrane assembly and having therein an outlet to permit fluid flow out of said housing,wherein said ...

MULTI-PATH SELECTOR VALVE

A multi-path selector valve used in liquid chromatography and other analytical methods for directing fluid along alternate paths of a flowstream. The selector valve has a stator and a rotor. The dynamic face of the stator has a plurality of openings arranged along an inner ring, a plurality of openings arranged along an outer ring, and an annular collection groove formed in the dynamic face. The inner ring, outer ring, and annular collection groove are concentric circles. The rotor's dynamic face is configured to mate with the rear face of the stator, and has two fluid flow paths. One fluid flow path has one end at a rotational center of the rotor and another end of the fluid flow path is configured to be aligned with a stator opening along the inner ring. The second fluid flow path has one end that is configured to be aligned with the annular collection groove and another end that is configured to be aligned with a stator opening along the outer ring. 1. A dual selector valve assembly , comprising: a plurality of openings arranged along an inner ring;', 'a plurality of openings arranged along an outer ring; and', 'an annular collection groove formed in the rear face; and, 'a stator having a front face and a rear face, wherein the rear face comprisesa rotor having a front face configured to mate with the rear face of the stator, the rotor having a first fluid flow path and a second fluid flow path, the first fluid flow path comprising a first end and a second end, wherein the first end of the first fluid flow path is at a rotational center of the rotor and the second end of the first fluid flow path is configured to be aligned with a stator opening along the inner ring, and the second fluid flow path comprising a first end and a second end, wherein the first end of the second fluid flow path is configured to be aligned with the annular collection groove and the second end of the second fluid flow path is configured to be aligned with a stator opening along the outer ...

MEANS AND METHODS FOR MINIMIZING SWEPT AND DEAD VOLUMES IN CHROMATOGRAPHIC APPLICATIONS

The present invention relates to a device for preventing band broadening and remixing of separated fractions, and associated method, comprising a chromatographic column coupled to a flow selector, such as a rotary valve, wherein said flow selector is connected to the distal end of said column such that the sum of post-column swept volume and post-column dead volume is less than 10 μL. Preferably, the column is directly plugged into the inlet port of the rotary valve and the sample is fractionated at the outlet port. 1. A device comprising or consisting of(a) a chromatographic column; and(b) a flow selector,wherein said flow selector is connected to the distal end of said column such that the sum of post-column swept volume and post-column dead volume is less than 10 μL.2. A kit comprising or consisting of(a) a chromatographic column; and(b) a flow selector,wherein said column and said flow selector are configured for a connection of said flow selector to the distal end of said column such that the sum of post-column swept volume and post-column dead volume is less than 10 μL.3. The kit of , furthermore comprising a manual comprising instructions for assembly of a device according to .4. The device of of the kit of or , wherein said chromatographic column(a) is empty; or(b) is filled with chromatographic material;and/orhas an inner diameter of less than 2 mm; preferably of 250 μm or less; or 200 μm or lessand/orhas a volume of 2 mL or less, preferably 100 μL or less.54. The device or kit of claim (b) , wherein said chromatographic material is selected from reversed phase , ion exchange , normal phase , hydrophilic interaction , affinity and size exclusion material.6. The device of any one of , or , or the kit of any one of to , wherein said selector is an n-way rotor valve , n preferably being 2 , 3 , 4 , 5 , 6 , 7 , 8 , 9 , 10 , 11 , 12 , 13 , 14 , 15 , 16 , 17 , 18 , 19 , 20 , 21 , 22 , 23 or 24.7. The device of any one of or to , or the kit of any one of to , ...

LIQUID CHROMATOGRAPHY ANALYTE REACTION AND ANALYSIS SYSTEM

In the present system and method, a conduit from a LC device continuously transports solvent, buffers, and analytes to the inlet of a solvent removal and analyte conversion device which evaporates the solvents, leaving non-volatile analytes for detection. The device comprises a rotating disk. The liquid chromatograph device can be any device using liquid chromatography to separate molecules. The solvents in the LC effluent can include, but are not limited to, water, methanol, acetonitrile, tetrahydrofuran, an acetone. After removal of the volatile components, the non-volatile analytes are converted with a concentrated energy source so that they may be detectable. 1. A system for the detection and quantification of organic molecules from a liquid chromatograph comprising:a liquid chromatograph having an outlet;a device comprising a heated rotating disk, an inlet for effluent from the liquid chromatograph with the inlet depositing the effluent onto the disk, a solvent vent for capturing volatilized gases, an inlet for gases such as air, helium or hydrogen, a source of concentrated energy, and a vent for receiving transformed analyte from the action of the concentrated energy;a conduit connecting the outlet of the chromatograph with the inlet on the device for receiving effluent from the chromatograph;a detector for detecting and quantifying organic molecules, the detector having an inlet; anda conduit connecting the vent in the device for receiving transformed analyte to the inlet of the detector.2. The system of claim 1 , wherein the rotating disk comprises a circular groove with the inlet depositing the effluent into the groove.3. The system of claim 1 , wherein the device is heated by a resistive heater.4. The system of claim 1 , wherein the device is sealed.5. The system of claim 4 , wherein the device is pressurized.6. The system of claim 4 , wherein the device is sealed with rubber and/or polymer seals.7. The system of claim 6 , wherein the seals are spring ...

SAMPLE DISPATCHING WITH FLUIDIC SAMPLE RETAINING

A sample dispatcher for a fluid separation apparatus includes a sampling path including a sampling volume, a sampling unit, and a retaining unit. The sampling unit receives a fluidic sample, and the sampling volume temporarily stores an amount of the received sample. The retaining unit receives and retains from the sampling volume at least a portion of the stored sample, and has different retention characteristics for different components of the sample. A switching unit is coupled to the sampling path, a sampling fluid drive, a mobile phase drive, and a separating device. In a feed injection configuration of the switching unit, the mobile phase drive, the separating device, and the sampling path are coupled together in a coupling point for combining a flow from the sampling fluid drive containing the fluidic sample retained by the retaining unit with a flow of the mobile phase from the mobile phase drive. 1. A sample dispatcher for a fluid separation apparatus , wherein the fluid separation apparatus comprises a mobile phase drive , configured for driving a mobile phase , and a separating device configured for separating a portion of a fluidic sample when comprised within the mobile phase , the sample dispatcher comprising: the sampling unit is configured for receiving the fluidic sample,', 'the sampling volume is configured for temporarily storing an amount of the received fluidic sample, and', 'the retaining unit is configured for receiving and retaining from the sampling volume at least a portion of the fluidic sample stored in the sampling volume, wherein the retaining unit comprises different retention characteristics for different components of the fluidic sample; and, 'a sampling path comprising a sampling volume, a sampling unit, and a retaining unit, whereina switching unit coupling to the sampling path, a sampling fluid drive, the mobile phase drive, and the separating device,wherein the sampling fluid drive is configured for moving at least a portion of ...

CPC DISTRIBUTION CHROMATOGRAPHY OF CANNABINOIDS

The invention relates to cannabinoids and their isolation and purification and to obtaining them by means of centrifugal partition chromatography. 111.-. (canceled)12. A method for separating and/or purifying cannabinoids from a cannabis extract , including at least one liquid-liquid centrifugal partition chromatography step , wherein a solvent from the group of cyclohexane , heptane , or octane is selected and is kept stationary by centrifugal force , including and a second non-miscible liquid phase.13. The method for separating and/or purifying cannabinoids from a cannabis extract according to claim 12 , wherein the first phase is n-heptane.14. The method for separating and/or purifying cannabinoids from a cannabis extract according to claim 12 , wherein the second phase is acetonitrile claim 12 , methanol claim 12 , ethylacetate or water.15. The method for separating and/or purifying cannabinoids from a cannabis extract according to claim 12 , selected from the group consisting of:n-heptane/acetonitrile, n-heptane/ethylacetate/acetonitrile, n-heptane/ethylacetate/t-butyl methyl ether/acetonitrile,n-heptane/ethylacetate/methanol/water,n-heptane/methanol/water, n-heptane/ethanol/water,n-heptane/acetone/water, n-heptane/methanol/acetonitrile,n-heptane/ethanol/acetonitrile,n-heptane/acetone/acetonitrile,n-heptane/chloroform/acetonitrile,n-heptane/chloroform/methanol, n-heptane/methanol,n-heptane/ethanol/methanol,n-heptane/n-butanol/acetonitrile, n-heptane/2-propanol/water, n-heptane/n-propanol/water, n-heptane/2-propanol/acetonitrile, n-heptane/n-propanol/acetonitrile, n-heptane/dichloromethane/acetonitrile,n-heptane/dichloromethane/methanol,n-heptane/tetrahydrofuran/acetonitrile,n-heptane/benzotrifluoride/acetonitrile, Cyclohexane/methanol/water,Cyclohexane/methanol/acetonitrile,Cyclohexane/t-butyl methyl ether/water, Cyclohexane/acetonitrile/water, andIsooctane/methanol, isooctane/methanol/water, Isooctane/ethyl acetate/methanol/water.16. The method for separating ...

MODULAR AUTOMATED CHROMATOGRAPHY SYSTEM

Valves, pumps, detectors, sample loops, fraction collectors and the like are individually incorporated into modules that are mountable at individual mounting sites on a base unit which also supports one or more chromatography columns. Each module includes fluid connections to other modules and a microcontroller joining the module to a computed and monitor through an electronic connector at each mounting site. The fluid connections between the modules and the column(s) are removed from the electronic connections and accessible to the user. A software platform may recognize the modules and their locations, coordinate fluid connections between the modules, and provide a variety of control, monitoring, data generating and data processing functions to generate chromatographic data. The software platform may also provide graphical tools for designing chromatographic methods from a library of phases. 1. A method of operating a chromatography system , the method comprising:receiving a plurality of modules into a mounting frame, wherein each of the plurality of modules comprises a fluid manipulation component and a microcontroller that transmits operational signals to the fluid manipulation component in response to commands received from outside the module, and wherein the mounting frame further includes a plurality of bays, each of the plurality of bays constructed to receive a single module, and each of the plurality of bays comprising a signal connector that couples to and communicates with the microcontroller of a module mounted in the bay, wherein at least some of the bays are constructed to receive single modules interchangeably with other modules, and wherein the fluid manipulation components are connected together by fluid lines in an arrangement for implementing a flow scheme;displaying a depiction of the flow scheme in the user interface, wherein the depiction of the flow scheme is arranged generally linearly with flow from left to right, regardless of the physical ...

LIQUID CHROMATOGRAPH MASS SPECTROMETER

The invention provides a liquid chromatograph mass spectrometer which prevents contamination of a pump and a column and can perform mass calibration without adding a complicated mechanism. This liquid chromatograph mass spectrometer includes a liquid chromatograph including a liquid feed pump configured to feed a mobile phase solvent, a mass spectrometer configured to analyze a mass of a sample, and a standard sample container configured to be connected in series with the liquid chromatograph and the mass spectrometer in a flow path that connects the liquid chromatograph and the mass spectrometer and configured to house a standard sample for mass calibration. 1. A liquid chromatograph mass spectrometer , comprising:a liquid chromatograph including a liquid feed pump configured to feed a mobile phase solvent;a mass spectrometer configured to analyze a mass of a sample; anda standard sample container configured to be installed in a flow path connecting the liquid chromatograph and the mass spectrometer, configured to be connected in series with the liquid chromatograph and the mass spectrometer, and configured to house a standard sample for mass calibration.2. The liquid chromatograph mass spectrometer according to claim 1 , wherein a cylindrical case that includes an injection hole at a first end and a discharge hole at a second end and that has a flow path direction as a longitudinal direction, and', 'a partition plate configured to be movable along an inner wall of the case and partitioning an inside of the case, and wherein, 'the standard sample container includesthe partition plate is configured to be movable in a direction of the discharge hole due to movement of a mobile phase solution from the injection hole.3. The liquid chromatograph mass spectrometer according to claim 1 , wherein a case that includes an injection hole at a first end and a discharge hole at a second end, and', 'a tube connected between the injection hole and the discharge hole in the case ...

Methods and Apparatus for Analyzing Samples and Collecting Sample Fractions

Methods and apparatus for analyzing a sample using at least one detector are disclosed. 2. A method of claim 1 , wherein said generating and collecting steps are performed during the chromatographic run.3. The method of claim 1 , including at least one destructive detector selected from the group consisting of evaporative light scattering detectors (ELSD) claim 1 , mass spectrometers (MS) claim 1 , condensation nucleation light scattering detectors (CNLSD) claim 1 , and corona discharge detectors.4. The method of claim 1 , wherein said chromatography system further comprises at least one non-destructive detector selected from the group consisting of optical absorbance detectors claim 1 , refractive index detectors (RID) claim 1 , fluorescence detectors (FD) claim 1 , chiral detectors (CD) claim 1 , and conductivity detectors.5. The method of claim 4 , wherein the non-destructive detector comprises at least one optical absorbance detector.6. The method of claim 5 , wherein the optical absorbance detector observes two or more optical wavelengths so as to produce two or more detector response values.7. The method of claim 1 , wherein the destructive detector comprises an evaporative light scattering detector.8. The method of wherein said step of moving is performed with use ofa splitter pump;a shuttle valve; ora combination of a splitter pump and a shuttle valve;wherein the splitter pump, the shuttle valve, or the combination are in fluid communication with the at least one destructive detector.9. The method of claim 1 , wherein said step of actively moving comprises moving an aliquot from first sample stream at a frequency of at least 1 aliquot every 10 seconds.10. A non-transitory computer readable medium having stored thereon computer executable instructions for performing the method of .11. An apparatus for detecting a sample using the method of .12. A chromatographic apparatus for detecting and collecting one or more components within a first sample stream ...

Columns and Methods of Use for Analytical Standards and Compounds

This invention employs columns and methods to apply external and internal standards and compounds. Analytical standard or compounds are adsorbed to a solid phase extraction media and are stored indefinitely. The standards or compounds remain stable on the solid phase extraction media without decomposing. The standards or compounds may be removed from the solid phase extraction media with a solvent. 1. A packed bed column comprising solid phase media and standard compounds ,wherein the standard compounds are adsorbed to said solid phase media, andwherein the column is substantially free of solvent.2. The packed bed column of claim 1 , wherein the column is under vacuum.3. The packed bed column of claim 1 , wherein the column is under inert gas.4. The packed bed column of claim 1 , wherein the column further comprises an apparatus capable of providing bidirectional flow.5. The packed bed column of claim 1 , wherein the column further comprises an apparatus capable of providing unidirectional flow6. The packed bed column of claim 1 , wherein the column is covered by an opaque covering.7. The packed bed column of claim 1 , wherein the column is a solid phase extraction column.8. The packed bed column of claim 1 , wherein the amount of solvent is less than 5% of the weight of the solid phase extraction media.9. The packed bed column of claim 1 , wherein the amount of solvent is less than 1% of the weight of the solid phase extraction media.10. A method of quantitating compounds in a sample claim 1 , comprising{'claim-ref': {'@idref': 'CLM-00001', 'claim 1'}, 'adsorbing compounds in the sample to the packed bed column of ,'}eluting compounds from the column with solvent, andanalyzing the eluted compounds,wherein the standard compounds adsorbed to the solid phase media are used as an internal standard.11. The method of claim 10 , wherein the adsorbed standards are sample compounds.12. The method of claim 10 , wherein the adsorbed standards are compounds.13. The method of ...

CHROMATOGRAPHIC PURIFICATION METHOD

A chromatographic purification method for the isolation of a desired product fraction from a mixture using 2 chromatographic columns, comprises, within one cycle to be carried out at least once, the following steps: a first batch step, wherein said columns are disconnected and a first column is loaded with feed and its outlet is directed to waste, and from a second column desired product is recovered and subsequently the second column is regenerated; a first interconnected step, wherein the outlet of the first column is connected to the inlet of the second column, the first column is loaded beyond its dynamic breakthrough capacity with feed, and the outlet of the second column is directed to waste, a second batch step analogous to the first batch step but with exchanged columns; and a second interconnected step, analogous to the first interconnected step but with exchanged columns. 115.-. (canceled)16. A chromatographic purification method for an isolation of target molecules from a feed consisting of the target molecules and impurities to be separated from the target molecules , the target molecules being adsorbed on an affinity chromatography material having immobilized ligands that bind specifically to the target molecules while letting the impurities pass by unaffected , the method using only two chromatographic columns consisting of a first column and a second column , the first column and the second column being loaded with the affinity chromatography material , the first column having a first column inlet and a first column outlet , the second column having a second column inlet and a second column outlet , the method comprising the following steps in order: the two chromatographic columns are disconnected,', 'the first column is loaded with the feed via the first column inlet at a first flow rate and the first column outlet is directed to a waste, and', 'from the second column, the target molecules are recovered via the second column outlet based on the ...

AUTOMATED SYSTEM FOR DETECTION OF SILICON SPECIES IN PHOSPHORIC ACID

Systems and methods are described to provide speciation of silicon species present in a remote sample for analysis. A method embodiment includes, but is not limited to, receiving a fluid sample containing inorganic silicon in the presence of bound silicon from a remote sampling system via a fluid transfer line; transferring the fluid sample to an inline chromatographic separation system; separating the inorganic silicon from the bound silicon via the inline chromatographic separation system; transferring the separated inorganic silicon and bound silicon to a silicon detector in fluid communication with the inline chromatographic separation system; and determining an amount of one or more of the inorganic silicon or the bound silicon in the fluid sample via the silicon detector. 1. A method of automatic inline chromatography of silicon contained in a fluid sample , comprising:receiving a fluid sample containing inorganic silicon in the presence of bound silicon from a remote sampling system via a fluid transfer line;transferring the fluid sample to an inline chromatographic separation system;separating the inorganic silicon from the bound silicon via the inline chromatographic separation system;transferring the separated inorganic silicon and bound silicon to a silicon detector in fluid communication with the inline chromatographic separation system; anddetermining an amount of one or more of the inorganic silicon or the bound silicon in the fluid sample via the silicon detector.2. The method of claim 1 , wherein the bound silicon is in a polymeric form.3. The method of claim 1 , wherein the bound silicon is present in the fluid sample at a concentration of at least 100 parts per million (ppm) in a phosphoric acid matrix.4. The method of claim 1 , further comprising:receiving one or more additional fluid samples; andtransferring the one or more additional fluid samples to the silicon detector.5. The method of claim 1 , wherein receiving a fluid sample containing ...

GAS SAMPLER

A gas sampler () is provided with a connection portion (C) connectable to a sample tank (), a sample loop (PL) for holding a sample gas introduced from the sample tank () to the connection portion (C), pneumatic switching valves (V to V) for switching a flow path connected to the sample loop (PL), a control piping () for transmitting a driving pressure to the switching valves (V to V), a pump () for suctioning an inside of the sample loop (PL), and a pressure accumulation tank () for accumulating the pressure generated by the operation of the pump () as a source pressure. 1. A gas sampler for supplying a sample gas to a column of a gas analyzer , comprising:a connection portion connectable to a sample tank filled with the sample gas;a sample holding portion arranged to accommodate the sample gas to be introduced into the connection portion from the sample tank;a plurality of pneumatic switching valves configured to configure different flow paths within the gas sampler respectively corresponding to different switching configurations of the pneumatic switching valves, at least some of the flow paths being connected to the sample holding portion;control piping configured to transmit driving pressure to the pneumatic switching valves to control a connection state of each pneumatic switching valve;a pump configured to apply suction inside the sample holding portion; anda tank configured to accumulate pressure generated by an operation of the pump for a source pressure from which the driving pressure is derived.2. The gas sampler as recited in claim 1 ,wherein the pneumatic switching valves are configured to configure a flow path for introducing the sample gas to the sample holding portion, a flow path for introducing a carrier gas to the sample holding portion, and a flow path for supplying the sample gas in the sample holding portion to a column.3. The gas sampler as recited in claim 1 ,wherein the tank is connected between a discharge port of the pump and the control ...

Modulated flame gas flow rates in flame-based detectors

Methods and apparatus for the modulation of flame gas stoichiometry to a flame-based detector for use in chromatographic separations are presented. As the total mass flow rate of mobile phase entering the flame-based detector changes (e.g., as a result of density programming in the separation), the mass flow rate of combustion gases to the detector are altered in proportion to the amount of mobile phase entering the detector. As a result, flame stability and sensitivity of the detector can be maintained by the methods and apparatus of the present disclosure.

PREPARATIVE COLUMN CHROMATOGRAPHY SYSTEM

A chromatography system comprising a mixing circuit or a mixing chamber, a bubble trap, a concentration detector and one or more pumps, characterized in that the bubble trap has a permanent opening at its highest point is described herein. Furthermore, a chromatography system is described, characterized in that it contains two concentration detectors the first of which is located in the mixing circuit or the mixing chamber and the second is located downstream of the main pump. 116-. (canceled)17: A chromatography system comprising:a mixing circuit or mixing chamber;a main pump located downstream of the mixing circuit or mixing chamber;a chromatography column located downstream of the main pump;a first concentration detector located in the mixing circuit or the mixing chamber; anda second concentration detector located downstream of the main pump.18: The chromatography system of claim 17 , further comprising a bubble trap comprising a permanent opening at the highest point.19: The chromatography system of claim 18 , wherein the bubble trap is located outside or downstream of the mixing circuit or the mixing chamber.20: The chromatography system of claim 18 , further comprising a first valve between the mixing circuit or mixing chamber and the bubble trap configured to regulate the volume flow into the bubble trap.21: The chromatography system of claim 20 , wherein the first valve is an adjustable valve.22: The chromatography system of claim 18 , wherein the bubble trap comprises a second valve configured to regulate the volume flow through the permanent opening.23: The chromatography system of claim 22 , wherein the second valve is an adjustable valve.24: The chromatography system of claim 17 , further comprising a bypass route around the chromatography column.26: The method of claim 25 , wherein the buffer composition is based on a predetermined gradient.27: The method of claim 25 , wherein the chromatography system further comprises a bypass route around the ...

STRUCTURE OF FLOW CELL ASSEMBLY FOR CHROMATOGRAPHY DETECTOR AND METHOD OF MANUFACTURING CAPILLARY

A structure of a flow cell assembly for a chromatography detector is used to generate a chromatograph of a sample, and includes a capillary extending in one direction, a housing that houses at least part of the capillary and a ferrule that holds the capillary in the housing. The capillary includes an inner tube formed of a material having a refractive index lower than that of water, and an outer tube formed of a material having strength higher than that of the material of the inner tube and has an outer diameter that is equal to or smaller than 5 mm. An outer peripheral surface of the inner tube and an inner peripheral surface of the outer tube adhere to each other, and a flow path through which a mobile phase including a sample flows is formed in the inner tube. 1. A structure of a flow cell assembly for a chromatography detector that is used to generate a chromatogram of a sample , comprising:a capillary extending in one direction;a housing that houses at least part of the capillary; anda ferrule that holds the capillary in the housing, whereinthe capillary includesan inner tube formed of a material having a refractive index lower than a refractive index of water, andan outer tube formed of a material having strength higher than strength of a material of the inner tube and has an outer diameter that is equal to or smaller than 5 mm, andan outer peripheral surface of the inner tube and an inner peripheral surface of the outer tube adhere to each other, and a flow path through which a mobile phase including a sample flows is formed in the inner tube.2. The structure of a flow cell assembly for a chromatograph detector claim 1 , according to claim 1 , whereina length of the capillary in the one direction is larger than 10 mm.3. The structure of a flow cell assembly for a chromatograph detector according to claim 2 , whereina length of the capillary in the one direction is equal to or larger than 30 mm.4. The structure of a flow cell assembly for a chromatography ...

LIQUID CHROMATOGRAPH INCLUDING PASSAGE SWITCH VALVE

A liquid chromatograph inc es: an analysis column; a sample loop that temporarily contains a mixed liquid of a sample and a mixture solvent; and a passage switch valve capable of switching a passage between a load position where the mixed liquid is temporarily held in the sample loop and an injection position where the mixed liquid held in the sample loop is sent to the analysis column. In the liquid chromatograph, a specific passage is formed in each of the load position and the injection position. 1. A liquid chromatograph comprising:a sample receiving part that receives a sample supplied by a sample supply pump;a mixture solvent supply pump for supplying a mixture solvent to be mixed with the sample at a predetermined mixture ratio;a sample loop that temporarily contains a mixed liquid of the sample and the mixture solvent, and has one end and an other end;an analysis column that performs separation on the sample into components;a detector that detects the components obtained by the separation in the analysis column;a mobile phase supply pump for sending a mobile phase to the analysis column; anda passage switch valve capable of switching a passage between a load position where the mixed liquid is temporarily held in the sample loop and an injection position where the mixed liquid held in the sample loop is sent to the analysis column, whereinthe passage switch valve has ports each of which is individually connected to: the sample receiving part; the mixture solvent supply pump; the one end and the other end of the sample loop; the analysis column; and the mobile phase supply pump,in the load position, passages a and b are formed, the passage a allowing communication between the ports respectively connected to the mixture solvent supply pump, the sample receiving part, and the one end of the sample loop, and the passage b allowing communication between the ports respectively connected to the mobile phase supply pump and the analysis column, and.in the injection ...

Coupled Analytical Instruments for Dual Mode FTIR/GC-FTIR

A system and method are disclosed for analyzing samples, which includes a spectrometry system for detecting components of a sample; a gas chromatography column for separating the components of a sample; a first sample unit for receiving a first sample from a sample source; and a second sample unit for receiving a second sample from a sample source. Each sample loop unit allows independent processing of samples in preparation for analysis. 1. An analysis system that is configurable for a spectrometric mode in which it performs spectrometric analysis on samples and a separation and spectrometric mode in which it first separates the samples into one or more respective components and then performs spectrometric analysis on the components of the samples.2. The analysis system as claimed in claim 1 , further comprising a chromatographic system for separating the samples into the components claim 1 ,3. The analysis system as claimed in claim 1 , further comprising at least one concentrator for concentrating components from the samples prior to separation.4. An analysis system that performs spectrometric analysis on samples simultaneously with concentrating samples for subsequent spectrometric analysis.5. An analysis system that performs spectrometric analysis on samples taken upstream of an abatement system while capturing samples downstream of the abatement system.6. An analysis system for analyzing samples from a sample source claim 1 , the system comprising:a spectrometry system for detecting one or more components of the samples;a gas chromatography system for separating the samples into the components;a concentrator for concentrating the samples; a) from the sample source to said spectrometry system;', 'b) from the sample source to the concentrator and directing concentrated samples to the gas chromatography system for separating the sample into the components and directing the separated components to the spectrometry system., 'wherein the system is configurable for ...

Chromatography System

A chromatography system for at least one of tangential flow chromatography and lateral flow chromatography comprising: an inlet; a functionalised adsorbent chromatography medium downstream of the inlet; an outlet downstream of the adsorbent chromatography medium; and a flow guide downstream of the inlet and upstream of the adsorbent chromatography medium and configured to distribute a flow of a liquid from the inlet across a width of the adsorbent chromatography medium; wherein the flow guide comprises a pattern of channels providing flow paths from the inlet to different parts of the adsorbent chromatography medium along the width of the adsorbent chromatography medium, wherein the pattern of channels is provided so as to reduce a difference in arrival time and/or flow velocity of liquid reaching the adsorbent chromatography medium across the width of the adsorbent chromatography medium.

HERMETIC MANIFOLD FOR ANALYTICAL INSTRUMENTS

A planar manifold includes a first, generally planar, layer having a plurality of apertures therethrough. A second layer has a plurality of apertures therethrough. A channel layer defines a plurality of channels therein and is interposed between the first layer and the second layer. At least one channel extends in a direction parallel to a plane of the planar manifold and couples an aperture of the first layer to an aperture of the second layer. 1. A planar manifold comprising:a first layer having a plurality of apertures therethrough, the first layer being generally planar;a second layer having a plurality of apertures therethrough;a channel layer defining a plurality of channels therein, the channel layer being interposed between the first layer and the second layer, wherein at least one channel extends in a direction parallel to a plane of the planar manifold and couples an aperture of the first layer to an aperture of the second layer; andwherein the planar manifold is constructed from polyimide.2. The planar manifold of claim 1 , wherein the first layer claim 1 , second layer claim 1 , and channel layer are laminated together.3. The planar manifold of claim 2 , wherein the entire planar manifold is constructed from adhesive-less polyimide.4. The planar manifold of claim 1 , and further comprising:a third layer having a plurality of apertures therethrough;a second channel layer defining a second plurality of channels and being interposed between the second and third layers, wherein at least one channel of the second plurality of channels extends in a direction parallel to a plane of the planar manifold and couples an aperture of the second layer to an aperture of the third.5. The planar manifold of claim 4 , wherein the first layer claim 4 , second layer claim 4 , third layer and both channel layers are laminated together.6. The planar manifold of claim 5 , wherein the entire planar manifold is constructed from adhesive-less polyimide.7. The planar manifold of ...

A Connection Device for a Separation System such as a Chromatography System

A connection device () configured for connecting a separation system () with a separation device (), wherein a fluid can be flowed from a separation system outlet connection (), through a bypass fluid path () of the connection device to the separation system inlet connection () while a connected separation device () is by passed. For achieving this at least one of an inlet and an outlet valve () provided in the connection device is provided in a closed state and at least one bypass valve (a, b) provided in the connection device is provided in an open state. 2. The connection device according to claim 1 , wherein the at least one bypass valve is of diaphragm type.3. A The connection device according to claim 1 , wherein said bypass valve is positioned in the bypass fluid path at a distance from the first and/or second fluid path of no more than 3 times claim 1 , or 2 times or 1 times a diameter of the first claim 1 , the second and/or the bypass fluid path.4. A The connection device according to claim 1 , wherein said inlet and outlet valves are of diaphragm type.5. The connection device according to claim 1 , wherein the inlet and outlet valves are positioned at a distance from junctions between the first and second fluid paths and the bypass fluid path claim 1 , of no more than 3 times claim 1 , or 2 times claim 1 , or 1 times a diameter of the first claim 1 , the second and/or the bypass fluid line.6. The connection device according to claim 1 , wherein it is pre-sterilized and the separation device inlet and outlet connections are provided with aseptic connectors.7. The connection device according to claim 1 , wherein it is pre-sterilized and the separation system inlet and outlet connections are provided with aseptic connectors.8. The connection device according to claim 1 , wherein said inlet valve claim 1 , said outlet valve and said at least one bypass valve are manually controllable into open and closed states.9. The connection device according to claim 1 , ...

PREPARATIVE CHROMATOGRAPH

A preparative chromatograph for collecting target components in a sample temporally separated in a column of a chromatograph in respective preparative containers is provided with: a detection unit having a flow cell accommodated in a housing and a detector for detecting a component that passes through the flow cell; a first pipe that connects the column and an inlet end of the flow cell; a flow path switching unit accommodated in the housing and configured to selectively flow the components that passed through the flow cell through a preparative flow path that is a flow path connected to the preparative containers or a waste liquid flow path; and a second pipe accommodated in the housing and connecting an outlet end of the flow cell and the flow path switching unit. 1. A preparative chromatograph for collecting target components in a sample temporally separated in a column of a chromatograph in respective preparative containers , comprising:a) a detection unit having a flow cell accommodated in a housing and a detector for detecting components that pass through the flow cell;b) a first pipe that connects the column and an inlet end of the flow cell;c) a flow path switching unit accommodated in the housing and configured to selectively flow the components that passed through the flow cell to a preparative flow path which is a flow path to be connected to the respective preparative containers or a waste liquid flow path; andd) a second pipe accommodated in the housing, the second pipe connecting an outlet end of the flow cell and the flow path switching unit.2. The preparative chromatograph as recited in claim 1 ,wherein the detection unit is an absorptiometer equipped with an LED light source.3. The preparative chromatograph as recited in claim 1 ,wherein the detection unit is equipped with an optical fiber that transmits irradiation light emitted from a light source arranged outside the housing and irradiated to the flow cell.4. The preparative chromatograph as ...

SYSTEM AND METHOD OF APPLIED RADIAL TECHNOLOGY CHROMATOGRAPHY

A system and method of applied radial technology chromatography using a plurality of beads is disclosed, with each bead comprising one or more pores therein having a diameter of about 250 Å to about 5000 Å, and each bead having an average radius between about 100 μm to about 250 μm. Also disclosed are processes for selecting beads for use in a radial flow chromatography column, and for purifying an unclarified feed stream using a radial flow chromatography column. 1. A radial flow chromatography column comprising:a plurality of beads, with each bead comprising one or more pores therein, andinterstitial channels formed between the beads,wherein each pore has a diameter of about 250 Å to about 5000 Å,wherein at least about 80% of the plurality of beads have a diameter of about 200 μm to about 500 μm andwherein the beads have an average radius R of about 100 μm to about 250 μm.2. The radial flow chromatography column of claim 1 , wherein the bead is made of a polymer claim 1 , glass claim 1 , alumina claim 1 , silica claim 1 , controlled pore glass (CPG) claim 1 , cellulose claim 1 , encapsulated iron particles claim 1 , encapsulated CPG claim 1 , or encapsulated silica.3. The radial flow chromatography column of claim 2 , wherein the bead is a polymer bead.4. The radial flow chromatography column of claim 1 , wherein any beads having r<0.414 R have been removed.5. The radial flow chromatography column of claim 1 , wherein any beads having r<0.225 R have been removed.6. The radial flow chromatography column of claim 1 , wherein the interstitial channels have: a tetrahedron site radius r=0.225 R claim 1 , an octahedron site radius r=0.414 R claim 1 , or both.7. The radial flow chromatography column of claim 1 , wherein the interstitial channels have a narrowest channel radius r=0.155 R.8. The radial flow chromatography column of claim 1 , wherein the beads are monodisperse.9. A process for selecting beads for use in a radial flow chromatography column comprising: a) ...

CPC DISTRIBUTION CHROMATOGRAPHY OF CANNABINOIDS

The invention relates to cannabinoids and their isolation and purification and to obtaining them by means of centrifugal partition chromatography. 1. A method for separating and/or purifying cannabinoids from a cannabis extract , including at least one liquid-liquid centrifugal partition chromatography step , wherein a solvent from the group of cyclohexane , heptane , or octane is selected and is kept stationary by centrifugal force , including and a second non-miscible liquid phase.2. The method for separating and/or purifying cannabinoids from a cannabis extract according to claim 1 , wherein the first phase is n-heptane.3. The method for separating and/or purifying cannabinoids from a cannabis extract according to claim 1 , wherein the second phase is acetonitrile claim 1 , methanol claim 1 , ethylacetate or water.4. The method for separating and/or purifying cannabinoids from a cannabis extract according to claim 1 , selected from the group:n-heptane/acetonitrile,n-heptane/ethylacetate/acetonitrile,n-heptane/ethylacetate/t-butyl methyl ether/acetonitrile,n-heptane/ethylacetate/methanol/water,n-heptane/methanol/water, n-heptane/ethanol/water,n-heptane/acetone/water, n-heptane/methanol/acetonitrile,n-heptane/ethanol/acetonitrile,n-heptane/acetone/acetonitrile,n-heptane/chloroform/acetonitrile,n-heptane/chloroform/methanol, n-heptane/methanol,n-heptane/ethanol/methanol,n-heptane/n-butanol/acetonitrile, n-heptane/2-propanol/water, n-heptane/n-propanol/water, n-heptane/2-propanol/acetonitrile, n-heptane/n-propanol/acetonitrile,n-heptane/dichloromethane/acetonitrile,n-heptane/dichloromethane/methanol,n-heptane/tetrahydrofuran/acetonitrile,n-heptane/benzotrifluoride/acetonitrile,Cyclohexane/methanol/water,Cyclohexane/methanol/acetonitrile,Cyclohexane/t-butyl methyl ether/water,Cyclohexane/acetonitrile/water,Isooctane/methanol, isooctane/methanol/water,Isooctane/ethyl acetate/methanol/water.5. The method for separating and/or purifying cannabinoids from a cannabis ...

Chromatography filter

A filter for a chromatography system includes a filter body having an exterior wall, a cavity, an interior wall surrounding the cavity and a channel extending between the exterior and interior walls. The channel passes a flow of a liquid from the cavity. The filter body is formed of a sintered metal oxide material and has a pore size that is less than a particle size to be filtered liquid. In some embodiments the filter body is formed of zirconium oxide or aluminum oxide. The filters reduce or eliminate the types of reactions that occur between conventional filter bodies and chromatographic solvents and samples. The filters can be used at different locations in a chromatography system to remove particles that may be present in sources of chromatographic solvents or introduced into the chromatographic system flow by chromatographic components, such as pumps, valves and other chromatographic system components.

MODULAR AUTOMATED CHROMATOGRAPHY SYSTEM

Valves, pumps, detectors, sample loops, fraction collectors and the like are individually incorporated into modules that are mountable in interchangeable manner at individual mounting sites on a base unit which also supports one or more chromatography columns. Each module includes fluid connections to other modules and a microcontroller joining the module to a computed and monitor through an electronic connector at each mounting site. The fluid connections between the modules and the column(s) are removed from the electronic connections and accessible to the user. A software platform recognizes the modules and their locations, coordinates fluid connections between the modules, and provides a variety of control, monitoring, data generating and data processing functions to generate chromatographic data. 1. A system for joining a plurality of fluid manipulation components into a flow scheme for directing fluids to and from a chromatographic separation device and for operating said joined fluid manipulation components according to a selected protocol , said system comprising:a plurality of modules, each said module comprising (a) one of said fluid manipulation components, and (b) a microcontroller that (i) encodes machine readable data characterizing the type of said fluid manipulation component, and (ii) transmits operational signals to said fluid manipulation component in response to commands received from outside said module; anda software platform in communication with the microcontroller of each module, said software being programmable for (1) receiving said machine-readable data, (2) recognizing from the received machine-readable data the types of the fluid manipulation components, (3) automatically mapping the plurality of fluid manipulation components to the flow scheme based on the received data, (4) guiding a user of the system in the joining of said fluid manipulation components according to said flow scheme, and for (5) causing said fluid manipulation ...

Improvements in and Relating to Fluid Path Networks

Disclosed is a fluid path network () comprising plural fluid paths () interconnected by plural nodal chambers (), three or more of said plural paths opening into each of said chambers, and said chambers being adapted the accept a removable fluid path modifying insert ( ). The network is employed in a chromatography system described herein. 1. A fluid path network comprising plural fluid paths interconnected by plural nodal chambers , three or more of said plural paths opening into each of said chambers , and further comprising a plurality of fluid path modifying inserts adapted to fit into any one of the chambers.2. A fluid path network as claimed in wherein the chambers are arranged in an array claim 1 , held generally in position by tubular walls of the fluid paths.3. A fluid path network as claimed in claim 1 , wherein the chambers are cylindrical and have at least one claim 1 , preferably two claim 1 , open ends to accept said inserts.4. A fluid path network as claimed in claim 1 , wherein the chambers are formed around an axis claim 1 , and said three or more fluid paths extend radially away from each chamber for example toward another chamber.5. A fluid path network as claimed in claim 4 , wherein the plural fluid path modifying inserts are removably insertable into said chambers claim 4 , for modifying the fluid paths claim 4 , and wherein: a first group of the inserts comprises a plug for obstructing one or more of the at least three fluid paths claim 4 , but connecting two or more of the at least three fluid paths: and/or a second group of the inserts comprises; a valve stem rotatable about the axis for selectively interconnecting two or more of the at least three fluid paths and/or a third group of the inserts comprises claim 4 , an adapter for transferring fluid away from or toward the network.6. A reconfigurable chromatography fluid path network for interconnecting chromatography fluidic units claim 4 , the network comprising plural tubular fluid paths ...

COLUMN OVEN

To diminish complication and difficulty in attachment and detachment of analytical columns to and from a column oven. The column oven includes, in a column housing part that includes a space for housing the plurality of analytical columns, a column rack that holds the plurality of analytical columns, an inlet switching valve, and an outlet connection portion. At least either the inlet switching valve or the outlet connection portion is formed integrally with the column rack and capable of being attached to and detached from the column housing part together with the column rack. 1. A column oven comprising:an inlet pipe;an outlet pipe;a column housing part including a space for housing a plurality of analytical columns therein;a column rack including a column holding portion for holding the plurality of analytical columns, and detachably disposed in the column housing part;an inlet switching valve provided in the space of the column housing part, the inlet switching valve includes an inlet port to which the inlet pipe is connected and a plurality of column inlet connection ports to which respective inlets of the plurality of analytical columns held by the column rack are connected, the inlet switching valve selects one column inlet connection port among the plurality of column inlet connection ports to connect the inlet port to the selected column inlet connection port; andan outlet connection portion provided in the space of the column housing part, the outlet connection portion includes a plurality of column outlet connection ports to which respective outlets of the plurality of analytical columns held by the column rack are connected and an outlet port to which the outlet pipe is connected, and the outlet connection portion connecting the analytical column connected to the inlet pipe to the outlet pipe,wherein at least one of the inlet switching valve and the outlet connection portion is formed integrally with the column rack and capable of being attached to and ...

MIXER BYPASS SAMPLE INJECTION FOR LIQUID CHROMATOGRAPHY

In a liquid chromatography system, a sample is injected into a column by flowing a solvent mixture from a mixer into the column along a solvent mixture flow path; and injecting a sample into the solvent mixture flow path downstream of the mixer. In another liquid chromatography system, a sample is injected into a column by loading an isolator fluid into a sample loop, loading a sample into the sample loop, and flowing the sample into the column as a plug in front of the isolator fluid. 1. A method for injecting a sample into a flow of a liquid chromatography system , the method comprising:providing a flow of a mobile phase in a mobile phase flow path at a mobile phase pressure;providing a fluidic junction in the mobile phase flow path, the fluidic junction communicating with a sample injection line and a chromatographic column;pressurizing a sample injection flow path, containing a sample, to a pressure matching the mobile phase pressure;connecting the sample injection flow path to the mobile phase flow path via the sample injection line and the fluidic junction;combining a flow of the pressurized sample injection flow path with the flow of the mobile phase at the fluidic junction to control a degree of sample-band focusing.2. The method of claim 1 , further comprisinginjecting the sample from the sample injection flow path into the fluidic junction and thereby bypassing a solvent mixer.3. The method of claim 1 , further comprising:flowing a first solvent and a second solvent into a mixer to form a solvent mixture;flowing the solvent mixture into the chromatographic column; andwhile flowing the solvent mixture, injecting the sample into the column downstream from the mixer.4. The method of claim 3 , whereinthe first solvent is selected from the group consisting of a weak solvent, water, an aqueous buffer fluid, and a supercritical or near supercritical fluid; andthe second solvent is selected from the group consisting of a strong solvent, an organic solvent, an ...

SYSTEMS, METHODS, AND DEVICES FOR PROVIDING PRESSURIZED SOLVENT FLOW

The present disclosure generally relates to systems, methods and devices for providing pressurized solvent flow in chromatography systems. 1. A chromatographic solvent delivery system comprising:at least one pressurized solvent source;at least one divert valve in fluid communication with the at least one solvent source; anda pump in fluid communication with the at least one divert valve,wherein the at least one divert valve is located in between the at least one solvent source and pump, and wherein the at least one divert valve is adapted for diverting at least one solvent flow stream to the pump or to an auxiliary flow path.29-. (canceled)10. The chromatographic solvent delivery system of claim 1 , wherein the at least one pressurized solvent source comprises:a solvent container configured to be pressurized;a mechanism to engage with the container and configured to pressurize the container; andat least one sensor configured to determine the pressure, volume or solvent flow from, inside the container.11. The chromatographic solvent delivery system of claim 10 , wherein the mechanism is a piston and the at least one sensor is configured to determine the pressure claim 10 , volume or solvent flow from the position of the piston.12. The chromatographic solvent delivery system of claim 1 , wherein the at least one pressurized solvent source includes a solvent container having a solvent outlet arranged below the solvent container claim 1 , such that the solvent at the solvent outlet is pressurized due to gravity.13. The chromatographic solvent delivery system of claim 1 , wherein the at least one pressurized solvent source includes: a plurality of solvent bays claim 1 , each bay comprising a force applicator and being configured to receive a solvent container claim 1 , each force applicator being individually controllable to apply a force to the corresponding solvent container in the corresponding bay.14. A chromatographic solvent delivery system comprising:at least one ...

HIGH-PERFORMANCE LIQUID CHROMATOGRAPHY

A chromatographic column assembly is described, comprising a channel and a flow inducer. The channel comprises an inlet for receiving a sample liquid and an outlet, and is adapted for separating the sample liquid into components when the sample liquid flows through the channel in an axial direction from the inlet to the outlet. The flow inducer is adapted for controllably inducing, when the sample fluid is flowing through the channel, a motion of the sample liquid in the channel in a plane substantially orthogonal to the axial direction. 117.-. (canceled)18. A chromatographic column assembly comprising:a channel comprising an inlet for receiving a sample liquid and an outlet, the channel being adapted for separating the sample liquid into components when said sample liquid flows through the channel in an axial direction from said inlet to said outlet; anda controllable flow inducer for inducing, when the sample fluid is flowing through the channel, a motion of said sample liquid in the channel in a plane substantially orthogonal to said axial direction.19. The chromatographic column assembly according to claim 18 , wherein said controllable flow inducer is a vortex flow inducer.20. The chromatographic column assembly according to claim 18 , wherein said controllable flow inducer comprises a plurality of electrodes arranged in or on said channel claim 18 , the plurality of electrodes being adapted for generating an electric field suitable for inducing said motion.21. The chromatographic column assembly according to claim 18 , wherein said controllable flow inducer comprises a plurality of electrodes arranged in or on said channel claim 18 , the plurality of electrodes inducing a motion by acting on charged and/or polarizable constituents of said sample liquid claim 18 , independent of the macroscopic flow of the sample liquid.22. The chromatographic column assembly according to claim 20 , further comprising an electrical insulator for electrically insulating said ...

MICRODEVICE FOR SEPARATING PLASMA FROM HUMAN BLOOD

A microdevice for separating plasma from human blood comprising a blood flow channel () connected to a corpuscles flow channel () and a plasma flow channel () through a curved construction channel (). Blood flow channel has width 150 to 400 μm and length 1-20 mm, constriction channel () has width 60 to 200 μm, length 0.157 to 3.15 mm and curvature angle 90-270° with inner radius 50 μm to 1 mm and outer radius 110 μm to 1.2 mm and corpuscles flow channel () has width 200 to 700 μm, length 0.5 to 5 mm and bend angle 40-70°. Plasma channel is sinewave shaped and has width 20-150 μm and length 10-50 mm. Channels have uniform depth 20-120 μm (FIG. ). 1. A microdevice for separating plasma from human blood , comprising a polymer material microchip having a microchannel pattern comprising a blood flow channel having a blood inlet end and a blood outlet end , a corpuscles flow channel having a corpuscles inlet end and a corpuscles outlet end and at least one plasma flow channel having a plasma inlet end and a plasma outlet end , the blood inlet end of the blood flow channel being connected to a blood reservoir , the corpuscles outlet end of the corpuscles flow channel being connected to a corpuscles reservoir and the plasma outlet end of the plasma flow channel being connected to a plasma reservoir , the blood outlet end of the blood flow channel being connected to the corpuscles inlet end of the corpuscles flow channel and plasma inlet end of the plasma flow channel through a curved constriction channel having one end connected to the blood outlet end of the blood flow channel and the other end connected to the corpuscles inlet end of the corpuscles flow channel and plasma inlet end of the plasma channel , wherein the other end of the constriction channel is contiguous with the plasma inlet end of the plasma flow channel , the corpuscles flow channel is angular having one limb with the corpuscles inlet end terminating into the constriction channel perpendicular thereto and ...

AUTOMATED FLUID HANDLING SYSTEM

Automated fluid handling system comprising a housing and two or more fluid handling units arranged as interchangeable modular components with an external fluidics section and an internal non fluidics section, and wherein the housing comprises a liquid handling panel with two or more of component positions for receiving said interchangeable modular components such that the external fluidics section is separated from the non fluidics section by the liquid handling panel. 114-. (canceled)15. A column valve for an automated liquid chromatography system , the column valve comprising:i. two or more pairs of column connection ports each for connecting a chromatography column therebetween;ii. an inlet port;iii. an outlet port;wherein the valve is configured for selective connection of a column connected to a pair of column connector ports by connecting the inlet port to one of the ports in the pair of column connector ports and the outlet port to the other port of the pair of column connector ports, and wherein the valve further comprises integrated pressure sensors integrated into the inlet and outlet ports of the valve, whereby the automated liquid chromatography system can measure the actual pressure over the connected column.16. The column valve of claim 15 , wherein the valve has a built-in bypass capillary that enables bypassing of connected columns.17. The column valve of claim 15 , wherein the valve is a motorized rotary valve.18. The column valve of claim 15 , wherein the flow direction can be set either from the top of the column to the bottom of the column claim 15 , or from the bottom of the column to the top of the column.19. The column valve of claim 15 , wherein the liquid chromatography system comprises a master control unit arranged to automatically detect fluid handing units when inserted into the fluid handing unit receiving positions.20. The column valve of claim 15 , the column valve forms part of a fluid handing unit claim 15 , the fluid handing unit ...

Platform for Native Liquid Chromatography-Mass Spectrometry

Disclosed are native liquid chromatography-mass spectrometry systems and methods of use. A native liquid chromatography-mass spectrometry system can include a liquid chromatography system capable of separating a sample; and an electrospray ionization mass spectrometry (ESI-MS) system in fluid communication with the liquid chromatography system, wherein the ESI-MS system comprises a multi-nozzle electrospray ionization emitter and a system for modifying a desolvation gas and a mass spectrometer, wherein the mass spectrometer is configured to receive ions and characterize mass to charge ratio of ions. 1. A native liquid chromatography-mass spectrometry system , comprising:a liquid chromatography system capable of separating a sample; andan electrospray ionization mass spectrometry (ESI-MS) system in fluid communication with the liquid chromatography system, wherein the ESI-MS system comprises a multi-nozzle electrospray ionization emitter, a system for modifying a desolvation gas, and a mass spectrometer, wherein the mass spectrometer is configured to receive ions and characterize mass to charge ratio of ions.2. The native liquid chromatography-mass spectrometry system of claim 1 , wherein the system for modifying a desolvation gas comprises a container having a cap and comprising an organic solvent and an additional chemical component comprising an acid or a base claim 1 , wherein the cap has an inlet line port and an outlet line port; a sheath gas inlet line for providing a sheath gas to the inlet line port; and a modified desolvation gas outlet line capable of connecting the modified desolvation gas outlet line port to the multi-nozzle electrospray ionization emitter.34-. (canceled)5. The native liquid chromatography-mass spectrometry system of claim 2 , wherein the acid is trifluoroacetic acid and wherein the base is triethylamine.67-. (canceled)8. The native liquid chromatography-mass spectrometry system of claim 2 , wherein the organic solvent is acetonitrile.9. ...

PREPARATIVE COLUMN CHROMATOGRAPHY SYSTEM

A chromatography system comprising a mixing circuit or a mixing chamber, a bubble trap, a concentration detector and one or more pumps, characterized in that the bubble trap has a permanent opening at its highest point is described herein. Furthermore, a chromatography system is described, characterized in that it contains two concentration detectors the first of which is located in the mixing circuit or the mixing chamber and the second is located downstream of the main pump. 1. A chromatography system comprising a column , a mixing circuit or a mixing chamber , a bubble trap , a concentration detector and one or more pumps , wherein the bubble trap has a permanent opening at the highest point.2. The chromatography system according to claim 1 , wherein the bubble trap is located outside or downstream of the mixing circuit or the mixing chamber.3. The chromatography system according to claim 1 , wherein the inflow to the bubble trap is adjustable.4. The chromatography system according to claim 3 , wherein the adjustment takes place by means of a valve.5. The chromatography system according to claim 1 , wherein the bubble trap is located within the mixing circuit or the mixing chamber.6. The chromatography system according to claim 1 , wherein the volume flow through the permanent opening can be adjusted.7. The chromatography system according to claim 6 , wherein the adjustment takes place by means of a valve.8. The chromatography system according to claim 1 , further comprising a column claim 1 , a first volume flow measuring device and a second volume flow measuring device claim 1 , wherein the first volume flow measuring device is located downstream of the permanent opening and the second volume flow measuring device is located after the column.9. The chromatography system according to claim 1 , further comprising a second concentration detector and a main pump claim 1 , wherein the second concentration detector is downstream of the main pump.10. The ...

Predicting Properties of Well Bore Treatment Fluids

Methods and systems for predicting properties of well bore treatment fluids are disclosed. An embodiment includes a method of predicting fluid properties comprising: determining an operational window for a well bore fluid system; collecting data at vertices of the operational window; and developing a model comprising predicted properties for a plurality of data points within the operational window, wherein developing the model uses Barycentric interpolation. 111-. (canceled)12. A method of servicing a well bore comprising:providing an optimized treatment fluid, wherein the optimized treatment fluid is based, at least in part, on a model developed using Barycentric interpolation; andintroducing the treatment fluid into a well bore.13. The method of claim 12 , wherein the treatment fluid is a spacer fluid introduced into a well bore between a cement composition and a drilling fluid.14. The method of claim 12 , further comprising determining an operational window for a well bore fluid system; collecting data at vertices of the operational window; anddeveloping the model comprising predicted properties for a plurality of data points within the operational window, wherein developing the model uses Barycentric interpolation.15. The method of claim 14 , wherein the two or more boundary conditions comprise mass ratio of weighting additive to water and mass ratio of viscosifier to water.16. The method of claim 14 , wherein the operational window comprises a triangular claim 14 , two-dimensional window.17. The method of claim 14 , wherein the operational window is defined as follows:{'sub': 1', '1', '2', '2', '3', '3, 'Vertex 1 (x, y), Vertex 2 (x, y), and Vertex 3 (x, y), wherein x is a first boundary condition for the well bore fluid system and y is a second boundary condition for the well bore fluid system.'}1820.-. (canceled)21. The method of claim 12 , wherein data from the well bore claim 12 , comprising data points claim 12 , is sent to a processor that is coupled to ...

Versatile Rotary Valve

A rotary valve comprising a stator with an inner stator face, and a rotor with an inner rotor face arranged in sealing contact with the inner stator face, the rotor is rotatably movable to a plurality of rotor positions about a rotational axis relative to the inner stator face, the stator comprises a plurality of connection ports each being in fluidic contact with a corresponding valve orifice at the inner stator face and the rotor comprises two or more interconnection paths for selective fluidic interconnection of said valve orifices with respect to the rotor position, wherein the stator comprises, an inlet port, an outlet port, a component feed port, a component return port, and wherein the interconnection paths in the rotor are arranged to:—in a first rotor position interconnect the inlet port with the outlet port,—in a second rotor position interconnect the inlet port with the component feed port and the component return port with the outlet port,—in a third rotor position interconnect the inlet port with the component return port and the component feed port with the outlet port. 1. A rotary valve comprising a stator with an inner stator face , and a rotor with an inner rotor face arranged in sealing contact with the inner stator face ,wherein the rotor is rotatably movable to a plurality of rotor positions about a rotational axis relative to the inner stator face,wherein the stator comprises a plurality of connection ports each being in fluidic contact with a corresponding valve orifice at the inner stator face and the rotor comprises two or more interconnection paths for selective fluidic interconnection of said valve orifices with respect to the rotor position,wherein the stator comprises an inlet port, an outlet port, a component feed port, anda component return port, and in a first rotor position interconnect the inlet port with the outlet port,', 'in a second rotor position interconnect the inlet port with the component feed port and the component return ...

BLEND VALVE

A blend valve assembly including a plurality of ports, a stator having at least one fluid flow path defined therein that extends between at least one port of the plurality of ports in communication with the primary fluid media and at least one port of the plurality of ports in communication with an intake of a testing device, and a rotor comprising at least one fluid flow path defined therein. The rotor is movable between a series of positions such that the blend valve assembly is operable to provide the intake of the testing device with only the primary fluid media and to provide the intake of the testing device with a blend of the primary fluid media and the secondary fluid media. 1. A blend valve assembly for a testing or measurement device , comprising:a plurality of ports comprising at least one port in communication with a primary fluid media, at least one port in communication with a secondary fluid media, and at least one port in communication with an intake of the testing or measurement device;a stator comprising a body with at least one fluid flow path defined therein that extends between the at least one port in communication with the primary fluid media and the at least one port in communication with the intake of the testing or measurement device, and that facilitates a substantially continuous flow of the primary fluid media to the intake of the testing or measurement device; anda rotor comprising a rotor body having at least one fluid flow path defined therein, wherein the rotor is movable between a series of positions to selectively place the at least one flow path defined in the rotor into and out of communication with selected ports of the plurality of ports, wherein the blend valve assembly is operable to provide the intake of the testing or measurement device with only the primary fluid media, and/or to provide the intake of the testing or measurement device with a blend of the primary fluid media and the secondary fluid media.2. The blend valve ...

Flow channel structure and measuring device for measurement target liquid

A flow channel structure includes a substrate including a supply flow channel that guides a measurement target liquid toward inside; a separation element accommodating unit that accommodates a separation element that separates components included in the measurement target liquid; and a detection unit that guides the measurement target liquid passing through the separation element accommodating unit, wherein measuring light for measuring information about the components is to be irradiated onto the measurement target liquid. The detection unit includes a measurement flow channel part that guides the measurement target liquid, an incident part that is provided at an end of the measurement flow channel part and that guides the measuring light toward inside the measurement flow channel part, and an emission part that is provided at the other end of the measurement flow channel part and that derives the measuring light from the measurement flow channel part.

SEPARATION/PURIFICATION APPARATUS

A preparative purification apparatus capable of adjusting a mixing ratio of a mobile phase and an elution solvent contained in a solution to be collected. A liquid feeding unit which feeds an elution solvent to an inlet of a trap column; a flow path switching unit which selectively connects an outlet of the trap column to one of a waste liquid flow path or a recovery flow path; a liquid feed amount measurement unit; and a flow path control unit which performs a control such that the flow path switching unit is connected to the recovery flow path when the amount of the elution solvent fed to the trap column reaches a predetermined initial waste liquid amount, and thereafter, the flow path switching unit is connected to the waste liquid flow path at a timing of reaching a predetermined solution recovery amount. 14-. (canceled)5. A preparative purification apparatus which causes a solution eluted from a liquid chromatograph to flow through a trap column to capture a target component contained in the solution in the trap column , and thereafter , causes an elution solvent different from a mobile phase of the liquid chromatograph to flow through the trap column to elute the target component from the trap column , the apparatus comprising:a) a liquid feeding unit which feeds the elution solvent to an inlet of the trap column;b) a flow path switching unit which selectively connects an outlet of the trap column to one of a waste liquid flow path and a recovery flow path;c) a liquid feed amount measurement unit which measures an amount of the elution solvent fed to the trap column by the liquid feeding unit; andd) a flow path control unit which controls the flow path switching unit to connect the outlet of the trap column to the recovery flow path at a timing when the amount of the elution solvent fed to the trap column, which is measured by the liquid feed amount measurement unit and, reaches a predetermined initial waste liquid amount, and thereafter, to connect the outlet ...

Analyte concentrator system and methods of use

Systems and methods for concentrating an analyte preparatory to analysis thereof include processing the effluent of an analyte concentrator to produce an eluent for eluting an analyte retained in the same or separate concentrator, and systems implementing the same. The analyte concentrator system connects the effluent outlet of an analyte concentrator column to an eluent generation module such that the substantially analyte-free effluent discharged from the analyte concentrator column passes fluidly into the eluent generation module. Eluent generated from the substantially analyte-free effluent in the eluent generation module is likewise substantially free of the analyte. The systems and methods can minimize and/or (substantially) eliminate background signal during analysis of the concentrated analyte.

Method and Apparatus for Chromatograph Nano-Flow Fractionator

A nano-flow fractionator apparatus, comprises: one or more sources of mobile phase solvent; a source of auxiliary solvent; a sample injection valve; a chromatographic column having an inner diameter of less than or equal to 75 micro-meters, a column inlet end and a column outlet end; a solvent fraction delivery line comprising: an inlet end that is configured to receive eluate that is emitted from the column outlet end and an outlet end that is configured to dispense the eluate to each of a plurality of sample fraction containers; a fluid junction configured to receive the eluate that is emitted from the column outlet end and to receive a flow of the auxiliary solvent that is delivered from the source of auxiliary solvent and to deliver the eluate and the flow of auxiliary solvent to the solvent fraction delivery line.

AUTOMATED SYSTEM FOR DETECTION OF SILICON SPECIES IN PHOSPHORIC ACID

Systems and methods are described to provide speciation of silicon species present in a remote sample for analysis. A method embodiment includes, but is not limited to, receiving a fluid sample containing inorganic silicon in the presence of bound silicon from a remote sampling system via a fluid transfer line; transferring the fluid sample to an inline chromatographic separation system; separating the inorganic silicon from the bound silicon via the inline chromatographic separation system; transferring the separated inorganic silicon and bound silicon to a silicon detector in fluid communication with the inline chromatographic separation system; and determining an amount of one or more of the inorganic silicon or the bound silicon in the fluid sample via the silicon detector. 116.-. (canceled)17. A silicon speciation system for analysis of a fluid sample , comprising:a first valve having a first valve configuration to receive a remote sample containing phosphoric acid from a remote sampling system into a holding line coupled to the first valve;a pump assembly fluidically coupled to the first valve, the pump assembly including one or more pumps fluidically coupled with the first valve in a second valve configuration to deliver the remote sample or a diluted remote sample from the first valve;a second valve fluidically coupled to the first valve, the second valve coupled with each of an analysis output line and a silicon speciation portion having a speciation column, the second valve having a first valve configuration that fluidically couples the first valve with the silicon speciation portion to separate one or more species of silicon in the remote sample or the diluted remote sample via the speciation column, the silicon speciation portion coupled with the analysis output line and further including an eluent pump configured to direct an eluent through the speciation column to transfer the one or more species of silicon to the analysis output line, the second valve ...

PREPARATIVE SEPARATION AND PURIFICATION DEVICE AND PREPARATIVE SEPARATION AND PURIFICATION METHOD

A target component is collected using a preparative separation and purification device having a holder for holding a trap column in which the target component has been captured, a liquid feeder for feeding a first solvent having compatibility with the water remaining in the trap column and a second solvent having low compatibility with water and high compatibility with the first solvent into the trap column, a flow-path switch for connecting the exit end of the trap column to a waste liquid flow path and a collection flow path, and a control unit for controlling the flow-path switch so that solution including water flows into the waste liquid flow path. 12-. (canceled)3. A preparative separation and purification device for allowing a solution including a target component and a trapping solvent to flow through a trap column to trap the target component in the trap column and allowing a solvent different from the trapping solvent to flow through the trap column to collect the target component trapped in the trap column , comprising:a) a holding means for holding the trap column so that an entrance end of the trap column is directed downward and an exit end thereof is directed upward;b) a liquid feeding means selectively connected to a first solvent source storing a first solvent having a lower boiling point than the trapping solvent and having compatibility with the trapping solvent remaining in the trap column and a second solvent source storing a second solvent having a lower boiling point than the trapping solvent, having a higher specific gravity than the trapping solvent and the first solvent, having high compatibility with the first solvent, and having low compatibility with the trapping solvent while the target component is trapped in the trap column held by the holding means and feeding any one of the first solvent and the second solvent to the entrance end of the trap column;c) a liquid feed control means for connecting the liquid feeding means to the second ...

Chromatography System

A chromatography system for at least one of tangential flow chromatography and lateral flow chromatography comprising: an inlet; a functionalised adsorbent chromatography medium downstream of the inlet; an outlet downstream of the adsorbent chromatography medium; and a flow guide downstream of the inlet and upstream of the adsorbent chromatography medium and configured to distribute a flow of a liquid from the inlet across a width of the adsorbent chromatography medium; wherein the flow guide comprises a pattern of channels providing flow paths from the inlet to different parts of the adsorbent chromatography medium along the width of the adsorbent chromatography medium, wherein the pattern of channels is provided so as to reduce a difference in arrival time and/or flow velocity of liquid reaching the adsorbent chromatography medium across the width of the adsorbent chromatography medium. 1. A chromatography system for at least one of tangential flow chromatography and lateral flow chromatography comprising:an inlet;a functionalised adsorbent chromatography medium downstream of the inlet;an outlet downstream of the adsorbent chromatography medium; anda flow guide downstream of the inlet and upstream of the adsorbent chromatography medium and configured to distribute a flow of a liquid from the inlet across a width of the adsorbent chromatography medium;wherein the flow guide comprises a pattern of channels providing flow paths from the inlet to different parts of the adsorbent chromatography medium along the width of the adsorbent chromatography medium, wherein the pattern of channels is provided so as to reduce a difference in arrival time and/or flow velocity of liquid reaching the adsorbent chromatography medium across the width of the adsorbent chromatography medium.2. The chromatography system of claim 1 , wherein the pattern of channels is arranged such that the flow paths have lengths within 50% of each other.3. The chromatography system of claim 1 , wherein ...

MANAGING FLUIDIC CONNECTIONS TO MICROFLUIDIC DEVICES

A method may include reducing fluid flow between a rotor and a microfluidic device. The method may further include reducing a sealing force between the rotor and the microfluidic device. The method may also include rotating the rotor relative to the microfluidic device, at the reduced sealing force, to change a fluid pathway therebetween. The method may additionally include reestablishing the sealing force to produce a fluid tight seal between the rotor and the microfluidic device. Moreover, the method may include reestablishing the fluid flow between the rotor and the microfluidic device. 1. A method comprising:reducing fluid flow between a rotor and a microfluidic device;reducing a sealing force between the rotor and the microfluidic device;rotating the rotor relative to the microfluidic device, at the reduced sealing force, to change a fluid pathway therebetween;reestablishing the sealing force to produce a fluid tight seal between the rotor and the microfluidic device; andreestablishing the fluid flow between the rotor and the microfluidic device.2. The method of claim 1 , further comprising coordinating the steps of reducing the fluid flow and reducing the sealing force between the rotor and the microfluidic device.3. The method of claim 1 , wherein reducing fluid flow comprises reducing the flow to zero flow.4. A method for controlling a chromatography system to prevent at least one of wear claim 1 , particulate formation claim 1 , leakage claim 1 , and composition disturbance associated with an interface between a rotor and a microfluidic device claim 1 , the method comprising:driving a linear actuator to push the microfluidic device into fluid tight contact with the rotor to create a sealing force at the interface between the rotor and the microfluidic device to prevent at least one of wear, particulate formation, leakage, and composition disturbance associated with the interface between the rotor and the microfluidic device;delivering a mobile phase into ...

SUPERCRITICAL FLUID CHROMATOGRAPH AND SUPERCRITICAL FLUID CHROMATOGRAPHY ANALYSIS METHOD

A supercritical fluid chromatograph includes an analysis flow path, a mobile phase liquid sender, a masking liquid sender, a sample injector, an analysis column and a back pressure adjustor. The mobile phase liquid sender sends a mobile phase in the analysis flow path. The masking liquid sender sends a masking liquid in the analysis flow path. The masking liquid is the liquid that causes an inner surface of a metal pipe constituting the analysis flow path to have a function of suppressing adsorption of a metal-adsorbing substance. The sample injector injects a sample into the analysis flow path. The analysis column is provided on the analysis flow path and separates the sample that is injected into the analysis flow path by the sample injector. The back pressure adjustor is provided at a downstream end of the analysis flow path and adjusts the pressure in the analysis flow path. 1. A supercritical fluid chromatograph comprising:an analysis flow path;a mobile phase liquid sender for sending a mobile phase in the analysis flow path;a masking liquid sender for sending a masking liquid, that forms a film suppressing adsorption of a metal-adsorbing substance on an inner surface of a metal pipe constituting the analysis flow path, in the analysis flow path;a sample injector for injecting a sample into the analysis flow path;an analysis column that is provided on the analysis flow path and separates a sample that is injected into the analysis flow path by the sample injector; anda back pressure adjustor that is provided at a downstream end of the analysis flow path and adjusts a pressure in the analysis flow path.2. The supercritical fluid chromatograph according to claim 1 , further comprising a masking-processing step executor configured to execute a masking-processing step of sending the masking liquid in the analysis flow path for a predetermined time using the masking liquid sender in a predetermined period of time in which analysis is not being carried out claim 1 , ...

POLLUTANT GENERATION SYSTEM AND MONITORING SYSTEM FOR WIND TUNNEL TESTS

The present invention discloses a pollutant generation system. The pollutant generation system includes a pollution source and a pollutant emitter. The pollutant emitter is connected to the pollution source. The pollution source is composed of two gases including air and methane. The flows of the gases are strictly controlled. Then, the gases enter a magnetic bead glass bottle. Due to the disturbance of magnetic beads to the flowing of the gases, the gases are sufficiently disordered, and the two gases are sufficiently mixed by using a spiral tube to generate a uniform and stable pollution source. 1. A pollutant generation system for wind tunnel tests , comprising:a pollution source; anda pollutant emitter being connected to the pollution source;wherein the pollutant emitter is arranged in a simulation area in a wind tunnel, a magnetic bead glass bottle is further arranged between the pollutant emitter and the pollution source, one end of the magnetic bead glass bottle is connected to the pollutant emitter, another end of the magnetic bead glass bottle is connected to the pollution source, and a spiral tube is further arranged between the magnetic bead glass bottle and the pollutant emitter.2. The pollutant generation system for wind tunnel tests according to claim 1 , wherein the pollution source comprises air and methane claim 1 , the air is supplied by an air pump claim 1 , the methane is supplied by a methane supply tank claim 1 , and both the air pump and the methane supply tank are connected to the magnetic bead glass bottle by pipelines.3. The pollutant generation system for wind tunnel tests according to claim 2 , wherein the pipelines for connecting the air pump and the methane supply tank to the magnetic bead glass bottle are provided with flowmeters claim 2 , and each of the flowmeters is arranged between the magnetic bead glass bottle and the pollutant emitter.4. A pollutant monitoring system for wind tunnel tests claim 2 , comprising:{'claim-ref': {'@ ...

Versatile Rotary Valve

A rotary valve comprising a stator with an inner stator face, and a rotor with an inner rotor face arranged in sealing contact with the inner stator face, the rotor is rotatably movable to a plurality of rotor positions about a rotational axis relative to the inner stator face, the stator comprises a plurality of connection ports each being in fluidic contact with a corresponding valve orifice at the inner stator face and the rotor comprises two or more interconnection paths for selective fluidic interconnection of said valve orifices with respect to the rotor position, wherein the stator comprises, an inlet port, an outlet port, a component feed port, a component return port, and wherein the interconnection paths in the rotor are arranged to: in a first rotor position interconnect the inlet port with the outlet port, in a second rotor position interconnect the inlet port with the component feed port and the component return port with the outlet port, in a third rotor position interconnect the inlet port with the component return port and the component feed port with the outlet port.

MANAGING FLUIDIC CONNECTIONS TO MICROFLUIDIC DEVICES

A system (e.g., a chromatography system) includes a rotor, a microfluidic device, a rotor driver, a clamping mechanism, and control electronics. The rotor defines a plurality of first fluid-conveying features. The micro fluidic device defines one or more channels and a plurality of second fluid-conveying features, in fluid communication with the one or more channels. The rotor driver is coupled to the rotor and is configured to rotate the rotor, relative to the microfluidic device, between a first position and a second position such that, in each of the positions, at least one of the first fluid-conveying features cooperates with at least one of the one or more channels to provide for fluid communication therebetween. The clamping mechanism is operable to provide a sealing force to establish a fluid-tight seal between the rotor and the microfluidic device. 1. A system comprising:a rotor defining a plurality of first fluid-conveying features;a microfluidic device defining one or more channels and a plurality of second fluid-conveying features, in fluid communication with the one or more channels;a rotor driver coupled to the rotor and configured to rotate the rotor, relative to the microfluidic device, between a first position and a second position such that, in each of the positions, at least one of the first fluid-conveying features cooperates with at least one of the one or more channels to provide for fluid communication therebetween;a clamping mechanism operable to provide a sealing force to establish a fluid-tight seal between the rotor and the microfluidic device; andcontrol electronics operatively associated with the clamping mechanism and configured to control operation of the clamping mechanism such that the sealing force is reduced during rotation of the rotor relative to the microfluidic device.2. The system of claim 1 , wherein the first fluid-conveying features are grooves.3. The system of claim 1 , wherein the rotor comprises a polymeric material claim ...

ONLINE CHROMATOGRAPHY AND ELECTROSPRAY IONIZATION MASS SPECTROMETER

Methods and system for protein characterization using online chromatography and electrospray ionization mass spectrometry are provided. 1. A method for characterizing an antibody-drug conjugate , said method comprising:contacting a sample including the antibody-drug conjugate to a chromatographic system having a size-exclusion chromatography resin;washing said size-exclusion chromatography resin using a mobile phase to provide an eluent including the antibody-drug conjugate; andcharacterizing the antibody-drug conjugate in said eluent using an electrospray ionization mass spectrometer under native conditions.2. The method of claim 1 , wherein the electrospray ionization mass spectrometer is coupled to the chromatographic system having the size-exclusion chromatography resin.3. The method of claim 1 , wherein the electrospray ionization mass spectrometer is a nano-electrospray ionization mass spectrometer.4. The method of claim 1 , wherein at least one three way-splitter is used to couple the electrospray ionization mass spectrometer to the chromatographic system having the size-exclusion chromatography resin.5. The method of claim 1 , wherein at least one three way-splitter is used to couple an ultraviolet detector to the chromatographic system having the size-exclusion chromatography resin.6. The method of claim 5 , wherein the eluent from washing the size-exclusion chromatography resin is introduced in the ultraviolet detector through the at least one three way-splitter at a flow rate of about 0.2 mL/min to about 0.4 mL/min.7. The method of claim 1 , wherein the mobile phase used to wash the size-exclusion chromatography resin comprises ammonium acetate.8. The method of claim 1 , wherein the mobile phase used to wash the size-exclusion chromatography resin comprises a volatile salt.9. The method of claim 1 , wherein the mobile phase used to wash the size-exclusion chromatography resin has a total concentration of about 100 mM.10. The method of claim 1 , wherein ...

Suppressors with eluent screens for use in ion chromatography

A suppressor for use in reducing a background signal while detecting analytes in a liquid sample for ion chromatography is described. The suppressor includes a central channel and two flanking regenerant channels. The central channel is formed with an eluent channel plate including a central cutout portion having a peripheral boundary portion. The peripheral boundary portion includes a recessed eluent plate height that is less than the eluent plate height. The peripheral boundary portion includes two or more notches or protrusions. An eluent screen is disposed in the central cutout portion, in which a peripheral border of the eluent screen has two or more corresponding notches or corresponding protrusions to the eluent channel plate.

SAMPLE AUTOMATION MANAGER

A liquid chromatography system includes a solvent delivery system, a sample manager including a sample delivery system in fluidic communication with the solvent delivery system, the sample delivery system configured to inject a sample from a sample-vial into a chromatographic flow stream, a liquid chromatography column located downstream from the sample delivery system, and a detector located downstream from the liquid chromatography column. The sample delivery system further includes a first needle drive including a first sample needle configured to extract the sample from the sample-vial and deliver the sample to the liquid chromatography column, and a first syringe in communication with the first sample needle configured to meter extraction of the sample from the sample-vial. The sample manager further includes a sample automation system that includes a second needle drive including a second sample needle configured to add a volume of reagent to the sample-vial. 1. A liquid chromatography system comprising:a solvent delivery system;a sample manager including a sample delivery system in fluidic communication with the solvent delivery system, the sample delivery system configured to inject a sample from a sample-vial into a chromatographic flow stream;a liquid chromatography column located downstream from the sample delivery system; anda detector located downstream from the liquid chromatography column, a first needle drive including a first sample needle configured to extract the sample from the sample-vial and deliver the sample to the liquid chromatography column; and', 'a first syringe in communication with the first sample needle configured to meter extraction of the sample from the sample-vial;, 'wherein the sample delivery system further includes 'a second needle drive including a second sample needle configured to add a volume of reagent to the sample-vial.', 'wherein the sample manager further includes a sample automation system that includes2. The liquid ...

GAS CHROMATOGRAPH APPARATUS

A gas chromatograph having a column which separates respective components contained in a gas sample introduced via a carrier gas over time, wherein an analysis mode and a standby mode can be switched and executed. The gas chromatograph apparatus comprises: a carrier gas flow path, a column protection gas flow path, a flow path switching part provided between the carrier gas flow path and the column protection gas flow path and a column so that only one of the carrier gas or a column protection gas is introduced into the column, and a flow path control part for controlling the flow switching part so as to introduce the carrier gas into the column at the time of the analysis mode and introduce the column protection gas into the column at the time of the standby mode. 1. A gas chromatograph apparatus having a column which separates respective components contained in a gas sample introduced via a carrier gas over time , wherein an analysis mode in which an analysis of said gas sample is executed and a standby mode in which an analysis is not executed can be switched and executed , said gas chromatograph apparatus comprising:a) a carrier gas flow path for introducing said carrier gas into said column;b) a column protection gas flow path for introducing a column protection gas separate from said carrier gas into said column;c) a flow path switching part provided between said carrier gas flow path and said column protection gas flow path and said column so that only one of said carrier gas or said column protection gas is introduced into the column; andd) a flow path control part for introducing said carrier gas into said column at the time of said analysis mode and introducing said column protection gas into said column at the time of said standby mode.2. The gas chromatograph apparatus according to claim 1 , comprising:e) a flow controller having at least one control mode from among a constant linear velocity mode, a constant flow volume mode, and a constant pressure ...

FLOW PATH SWITCHING VALVE

A base and a stator forming a housing are joined by a stator position adjustment member. The adjustment member is a ring-shaped member having an inner diameter that is equal to the outer forms of the base and the stator, and threads opposite to each other are provided to an upper portion and a lower portion of the inner circumferential surface, along the circumferential direction. A thread for being screwed with the thread is provided, in the circumferential direction, to each of an upper portion of an outer circumferential surface of the base and a lower portion of an outer circumferential surface of an outer wall member of the stator. When the adjustment member is rotated, the base and the stator move in the direction of separating from each other or in the direction of coming close to each other. 1. A flow path switching valve comprising:a base forming a lower portion of a housing, the base having a thread provided at an upper portion of an outer circumferential surface and in a circumferential direction;a stator forming an upper portion of the housing, the stator including a plurality of ports for connecting flow paths at an upper surface, the ports being open to a surface that is an upper flat surface inside the housing;a rotor, accommodated inside the housing, having a flat surface that is in contact with the upper flat surface inside the housing, the rotor being provided with a switching groove, at the flat surface, that is a flow path for communicating the plurality of ports of the stator;a rotor rotating mechanism for holding the rotor and rotating the rotor;an elastic member, inserted between the base and the rotor in a compressed manner, for biasing the rotor toward the upper flat surface inside the housing by an elastic force; anda stator position adjustment member that is a ring-shaped member provided with a thread, at a lower portion of an inner circumferential surface, that is to be screwed with the thread provided at the outer circumferential surface ...

AUTOMATED FLUID HANDLING SYSTEM

Automated fluid handling system comprising a housing and two or more fluid handling units arranged as interchangeable modular components with an external fluidics section and an internal non fluidics section, and wherein the housing comprises a liquid handling panel with two or more of component positions for receiving said interchangeable modular components such that the external fluidics section is separated from the non fluidics section by the liquid handling panel. 114-. (canceled)15. A method of modifying a fluid path in an automated liquid chromatography system comprising at least four interchangeable modular components comprising;interchanging at least two of the interchangeable modular components in a housing unit comprising at least four component receiving positions arranged in a two dimensional array, so as to allow for modification of the liquid chromatography fluid path among the at least four interchangeable modular components;wherein each of the at least four interchangeable modular components comprises a CPU unit for independently performing fluid control operations in response to instructions from the system when installed in a receiving position of the housing unit.16. The method of claim 15 , wherein the interchanging of the at least four modular components optimizes the fluid flow path for the liquid chromatography.17. The method of claim 16 , wherein the positioning of the modular component is optimized through shortening the fluid flow path.18. The method of claim 15 , wherein the interchanging of the modular components includes connecting each of the at least four interchangeable modular components to a system BUS.19. The method of claim 15 , wherein the liquid chromatography system further comprises an expansion housing unit that includes a plurality of additional modular component receiving positions claim 15 , each receiving position being capable of receiving the interchangeable modular components.20. The method of claim 15 , wherein the ...

USE OF AN ACOUSTIC WAVE IN A CHROMATOGRAPHY SYSTEM

The present invention relates to a method comprising using an acoustic wave in a chromatography system. The present invention also relates to a corresponding system and a corresponding use. The system may comprise a surface acoustic wave assembly, wherein the surface acoustic wave assembly comprises a sender unit comprising a sender transducer for sending an acoustic wave and a detection unit for detecting the acoustic wave, a substrate configured for propagation of the acoustic wave, wherein the sender transducer is connected to the substrate, wherein the substrate comprises a substrate section for propagation of the wave from the sender transducer, wherein this substrate section comprises a substrate surface, wherein the surface acoustic wave assembly further comprises at least one channel for conducting fluid, wherein this channel is partly defined by the substrate surface. 1. A system for using an acoustic wave in a chromatography system , wherein the system comprises a surface acoustic wave assembly , wherein the surface acoustic wave assembly comprisesa sender unit comprising a sender transducer for sending an acoustic wave and a detection unit for detecting the acoustic wave,a substrate configured for propagation of the acoustic wave,wherein the sender transducer is connected to the substrate,wherein the substrate comprises a substrate section for propagation of the wave from the sender transducer, wherein this substrate section comprises a substrate surface,wherein the surface acoustic wave assembly further comprises at least one channel for conducting fluid, wherein this channel is partly defined by the substrate surface.2. The system according to claim 1 , wherein the system further comprises a measuring cell claim 1 , and wherein the at least one channel is defined by the substrate surface and by the measuring cell claim 1 , and wherein the measuring cell is disconnectable from the substrate.3. The system according to claim 1 , wherein the detection unit ...

PREPARATIVE SEPARATION AND PURIFICATION DEVICE AND PREPARATIVE SEPARATION AND PURIFICATION METHOD

A target component is collected using a preparative separation and purification device having a holder for holding a trap column in which the target component has been captured, a liquid feeder for feeding a first solvent having compatibility with the water remaining in the trap column and a second solvent having low compatibility with water and high compatibility with the first solvent into the trap column, a flow-path switch for connecting the exit end of the trap column to a waste liquid flow path and a collection flow path, and a control unit for controlling the flow-path switch so that solution including water flows into the waste liquid flow path. 1a) feeding a first solvent to an entrance end of the trap column for a predetermined time while the target component is trapped in the trap column held so that the entrance end of the trap column is directed downward and an exit end thereof is directed upward, the first solvent having a lower boiling point than the trapping solvent and having compatibility with the trapping solvent remaining in the trap column;b) feeding a second solvent to the entrance end of the trap column, the second solvent having a lower boiling point than the trapping solvent, a higher specific gravity than the trapping solvent and the first solvent, having high compatibility with the first solvent, and having low compatibility with the trapping solvent; andc) allowing a solution including the trapping solvent to flow through a waste liquid flow path when the solution is discharged from the exit end of the trap column, allowing a solution including the second solvent to flow through a collection flow path when the solution is discharged from the exit end of the trap column, and switching the flow path of the solution discharged from the exit end from the waste liquid flow path to the collection flow path at a predetermined timing until the solution including the second solvent starts to be discharged after a solution including the first ...

LIQUID CHROMATOGRAPH MASS SPECTROMETER

A lid member of a valve device is detachably attached to a main body housing fixed to a casing . A connection pipe is attached to the lid member . A pipe fitting is connected to a pipe fixed to the casing . When the lid member is mounted to the main body housing , the connection pipe is inserted in the pipe fitting connected to the pipe . Thus, the lid member can be mounted to the main body housing , with the lid member disposed at a fixed position relative to the main body housing . When the lid member is detached from the main body housing , the connection pipe is separated from the internal of the pipe fitting , and thus, the pipe connected to the pipe fitting does not move together with the lid member , and is maintained to be disposed at the fixed position in the casing . As a result, the pipe can be prevented from hindering operation of an operator. 1. A liquid chromatograph mass spectrometer that is a combination of a liquid chromatograph and a mass spectrometer , the liquid chromatograph separating components in a sample , the mass spectrometer performing mass spectrometry on the components separated by the liquid chromatograph , the mass spectrometer comprising:a mass spectrometry section that performs the mass spectrometry;a casing that accommodates the mass spectrometry section;a valve device that is interposed in a flow path for the sample from the liquid chromatograph to the mass spectrometry section and is attached to the casing; anda pipe fitting to which a liquid discharge pipe that communicates with a liquid discharge section is connected, wherein a hollow main body housing that is attached in the casing and has an opening section;', 'a flow path switching mechanism that is accommodated in the main body housing and switches the flow path;', 'a lid member that is detachably attached to the main body housing, includes a flow path connection section to which the flow path from the liquid chromatograph is connected, and closes the opening section of the ...

METHOD AND APPARATUS FOR SCALING BETWEEN CHROMATOGRAPHIC SYSTEMS USING HIGHLY COMPRESSIBLE FLUIDS

Methods for transferring a carbon dioxide based separation procedure from a reference chromatographic system to a target chromatographic system involve alternative techniques for determining system pressure drops not attributable to the column. One technique involves leveraging experimental chromatography to develop a correction factor that is a function of at least one correction coefficient and at least one ratio of the differential analyte retention time to the retention time in the reference system. Another technique involves leveraging other experimental measurements of tubing pressure drops under various condition to develop a lookup table that can be used to identify likely tubing pressure drops in the target system. A third technique leverages knowledge of the separation procedure and the target system and the likely nature of the relevant flow to calculate tubing pressure drops in the target system. 1. A method for efficiently transferring a carbon dioxide based separation procedure from a reference chromatographic system to a field chromatographic system , the method comprising:(a) identifying an average system pressure, an average column pressure, and an elution time for at least one analyte in a carbon dioxide based separation in the reference chromatographic system;(b) modifying at least one characteristic of the tubing between a pump outlet and a column inlet or between a column outlet and a back pressure regulator inlet in the reference chromatographic system to create a modified reference chromatographic system;(c) identifying an average system pressure, an average column pressure, and an elution time for the at least one analyte in the carbon dioxide based separation in the modified reference chromatographic system;(d) calculating an elution time differential and a correction coefficient for the at least one analyte in the carbon dioxide based separation; and(e) identifying an average system pressure and an elution time for the at least one analyte ...

Manifold Connection Assembly

A manifold assembly has a manifold and can be located between a block and a plate, and serve as a stator for a valve or other component. The manifold, block or plate can have a plurality of ports either integral thereto or removably connected thereto, with the ports adapted to receive and sealingly engage with a tube having an inner tube layer, an outer tube layer, a sleeve, a tip portion, and a nut. Each of the nut, sleeve, inner and outer tubing layers, and tip portion have a passageway therethrough, with at least the passageways in the sleeve, tip portion, outer tube layer, and nut adapted to allow the inner tube layer to pass therethrough or extend over the inner layer. The ends of the tip portion and inner layer together can define a substantially flat surface which can form a seal in a flat-bottomed port of the manifold, block, or plate, which can be used in a component in an analytical instrument system, including for example a liquid chromatography system. The nut, tube, ferrule, and transfer tube or liner tube may comprise biocompatible materials. In addition, the nut may have a slot, such as a slot adapted to allow the tube and the nut to be easily and quickly separated or to allow a portion of the tube to be easily and quickly inserted in the nut. 1. A manifold assembly comprising:at least one nut having a passageway therethrough and having a first end and a second end;a block adapted to removably hold a manifold and having a plurality of ports therein, wherein each of the ports are adapted to removably and sealingly engage with said nut and an end of a tube;a manifold having a plurality of inlet ports therein, wherein at least a portion of said manifold is attached to said block, and wherein each of the ports of said block is in fluid communication with one of the inlet ports of said manifold;a tube having a passageway therethrough and adapted to fit in a passageway extending through said nut, wherein said tube has a first end and a second end;a transfer ...

CHROMATOGRAPH SAMPLER AND METHOD FOR OPERATING CHROMATOGRAPH SAMPLER

In order to, in a chromatograph sampler, regulate the pressure of a sample fluid filled in a constant volume tube to be as constant as possible and ensure the reproducibility of measurement, the chromatograph sampler is configured to provide a bypass line between a sample introduction line and a sample discharge line in parallel with the constant volume tube, and when filling the sample fluid in the constant volume tube, flow the sample fluid also through the bypass line in parallel. 1. A chromatograph sampler adapted to supply a sample fluid as a measurement target to a column of a chromatograph , the chromatograph sampler comprising:a constant volume tube having a pair of fluid inlet/outlet ports;a sample introduction line into which the sample fluid is introduced from a supply source of the sample fluid;a sample discharge line from which the sample fluid is discharged to a discharge space for the sample fluid;a line switching mechanism adapted to switch between a first mode in which any one of the fluid inlet/outlet ports of the constant volume tube is connected to the sample introduction line, the other is connected to the sample discharge line, and the sample fluid is filled in the constant volume tube while circulating, and a second mode in which both of the fluid inlet/outlet ports are respectively disconnected from the sample introduction line and the sample discharge line and any one of the fluid inlet/outlet ports is connected to the column;a control mechanism adapted to keep the line switching mechanism in the first mode to fill the sample fluid in the constant volume tube, and then switch the line switching mechanism to the second mode to introduce the sample fluid in the constant volume tube into the column; anda bypass line of which one end is connected to the sample introduction line and the other end is connected to the sample discharge line.2. The chromatograph sampler according to claim 1 , configured such that:the line switching mechanism is one ...

LIQUID CHROMATOGRAPHY MEASUREMENT METHOD, LIQUID CHROMATOGRAPHY MEASUREMENT INSTRUMENT, AND LIQUID CHROMATOGRAPHY MEASUREMENT PROGRAM STORAGE MEDIUM

A liquid chromatography measurement method includes: switching between a first measurement mode using a liquid chromatography method in which hemoglobin A1c and a hemoglobin variant are measured in a measurement sample by sequentially delivering a first component-separating eluent, a second component-separating eluent and a wash eluent to an analytical column, and a second measurement mode using the liquid chromatography method in which the hemoglobin A1c is measured by sequentially delivering the first component-separating eluent and the wash eluent to the analytical column; delivering the wash eluent in the first measurement mode prior to an influence from the second component-separating eluent disappearing such that a first retention time of the hemoglobin A1c in the first measurement mode and a second retention time of the hemoglobin A1c in the second measurement mode are substantially the same as each other; and delivering the first component-separating eluent after the wash eluent. 1. A liquid chromatography measurement method , comprising:switching between a first measurement mode using a liquid chromatography method in which hemoglobin A1c and a hemoglobin variant are measured in a measurement sample by sequentially delivering a first component-separating eluent, a second component-separating eluent and a wash eluent to an analytical column, and a second measurement mode using the liquid chromatography method in which the hemoglobin A1c is measured by sequentially delivering the first component-separating eluent and the wash eluent to the analytical column;delivering the wash eluent in the first measurement mode prior to an influence from the second component-separating eluent disappearing such that a first retention time of the hemoglobin A1c in the first measurement mode and a second retention time of the hemoglobin A1c in the second measurement mode are substantially the same as each other; anddelivering the first component-separating eluent after the ...

AUTOMATED FLUID HANDLING SYSTEM

Automated fluid handling system comprising a housing and two or more fluid handling units arranged as interchangeable modular components with an external fluidics section and an internal non fluidics section, and wherein the housing comprises a liquid handling panel with two or more of component positions for receiving said interchangeable modular components such that the external fluidics section is separated from the non fluidics section by the liquid handling panel. 114-. (canceled)15. A method of modifying a fluid path in an automated liquid chromatography system comprising at least four interchangeable modular components comprising;interchanging at least two of the interchangeable modular components in a housing unit comprising at least four component receiving positions arranged in a two dimensional array, so as to allow for modification of the liquid chromatography fluid path among the at least four interchangeable modular components;wherein each of the at least four interchangeable modular components comprises a CPU unit for independently performing fluid control operations in response to instructions from the system when installed in a receiving position of the housing unit.16. The method of claim 15 , wherein the interchanging of the at least four modular components optimizes the fluid flow path for the liquid chromatography.17. The method of claim 16 , wherein the positioning of the modular component is optimized through shortening the fluid flow path.18. The method of claim 15 , wherein the interchanging of the modular components includes connecting each of the at least four interchangeable modular components to a system BUS.19. The method of claim 15 , wherein the liquid chromatography system further comprises an expansion housing unit that includes a plurality of additional modular component receiving positions claim 15 , each receiving position being capable of receiving the interchangeable modular components.20. The method of claim 15 , wherein the ...

AUTOMATED FLUID HANDLING SYSTEM

Automated fluid handling system comprising a housing and two or more fluid handling units arranged as interchangeable modular components with an external fluidics section and an internal non fluidics section, and wherein the housing comprises a liquid handling panel with two or more of component positions for receiving said interchangeable modular components such that the external fluidics section is separated from the non fluidics section by the liquid handling panel. 114-. (canceled)15. A method of modifying a fluid path in an automated liquid chromatography system comprising at least four interchangeable modular components comprising;interchanging at least two of the interchangeable modular components in a housing unit comprising at least four component receiving positions arranged in a two dimensional array, so as to allow for modification of the liquid chromatography fluid path among the at least four interchangeable modular components;wherein each of the at least four interchangeable modular components comprises a CPU unit for independently performing fluid control operations in response to instructions from the system when installed in a receiving position of the housing unit.16. The method of claim 15 , wherein the interchanging of the at least four modular components optimizes the fluid flow path for the liquid chromatography.17. The method of claim 16 , wherein the positioning of the modular component is optimized through shortening the fluid flow path.18. The method of claim 15 , wherein the interchanging of the modular components includes connecting each of the at least four interchangeable modular components to a system BUS.19. The method of claim 15 , wherein the liquid chromatography system further comprises an expansion housing unit that includes a plurality of additional modular component receiving positions claim 15 , each receiving position being capable of receiving the interchangeable modular components.20. The method of claim 15 , wherein the ...

DUAL MODE SAMPLE MANAGER

Described is a dual mode sample manager for a liquid chromatography system. The dual mode sample manager includes a sample needle, a sample loop, a metering pump, a needle seat and first and second valves. Each valve is configurable in two valve states to enable two modes of operation. In one mode, sample acquired and stored in the sample needle is injected into a chromatography system flow and, in the other mode, sample acquired through the sample needle and stored in the sample loop is injected into the chromatography system flow. The automated switching of the sample manager between the two modes of operation avoids the need for maintaining two separate liquid chromatography systems or manual reconfiguration of a chromatography system for users desiring the capability of both modes of operation. 1. A dual mode sample manager for a liquid chromatography system , comprising:a sample needle to acquire a sample from a sample source for injection into a chromatography system flow;a sample loop to store the sample for injection into the chromatography system flow;a metering pump controllable to draw or dispense a volume of a liquid;a needle seat configured to fluidically couple the sample needle to a fluidic path;a first valve having a plurality of first valve ports and being configurable in a first valve state wherein one of the first valve ports is configured in fluidic communication with at least one of the other first valve ports and configurable in a second valve state wherein the one of the first valve ports is configured in fluidic communication with a different one of the other first valve ports, the first valve being in fluidic communication with the metering pump and configured to receive a wash solvent and a chromatography system flow, and to deliver the received chromatography system flow; anda second valve having a plurality of second valve ports and being configurable in the first valve state wherein one of the second valve ports is configured in fluidic ...

Method for liquid chromatographic mass spectrometry and liquid chromatograph mass spectrometer

In an LC/MS analysis of a sample containing various compounds, additive supply pumps 164A and 164B in a post-column adding unit 16 draw and supply different kinds of additives A and B from containers 163A and 163B, respectively. The additives are mixed into an eluate through T-joints 162 and 161. A preferable combination of the additives is the combination of DMSO which produces the effect of gathering charge states and 2-propanol which produces the effect of promoting atomization or vaporization of droplets. By mixing the two additives into the eluate while mixing them at an appropriate flow-rate ratio according to a previously determined flow-rate program, the ionization efficiency can be nearly optimized for each compound during the process of generating ions by spraying electrically charged droplets of the eluate from an ESI spray 21. Consequently, the detection sensitivity becomes higher than conventional levels.

CONTINUOUS AND SEPARATING GAS ANALYSIS

A portable gas analysis apparatus for conducting a gas flow, in particular for highly volatile compounds, includes a seeker measurement path and a separation measurement path. The seeker measurement path extends from a sample gas inlet opening to a first air exit opening, wherein a connecting path branches off from the seeker measurement path to a separation measurement path, and said separation measurement path extends from the connecting path to a second air exit opening and is connected to a carrier gas inlet opening, wherein the gas analysis apparatus has a control element which is designed for reversing the gas flow in the connecting path. 1. A portable gas analysis apparatus comprising a seeker measurement path and a separation measurement path wherein the seeker measurement path extends from a sample inlet opening to a first air exit opening , wherein a connecting path branches off from the seeker measurement path to a separation measurement path , and the separation measurement path extends from the connecting path to a second air exit opening and is connected to a carrier gas inlet opening , wherein a separation detector is arranged at the separation measurement path , wherein the gas analysis apparatus has a control element configured for reversing the gas flow in the connecting path.2. The portable gas analysis apparatus of claim 1 , wherein the separation detector is configured for determining an amount of highly volatile compounds.3. The portable gas analysis apparatus of claim 1 , wherein the separation detector comprises a separation column claim 1 , and the separation column is a multi-capillary column.4. The portable gas analysis apparatus of claim 1 , wherein the seeker measurement path claim 1 , the separation measurement path as far as the separation detector claim 1 , and the connecting path are in each case free of valves.5. The portable gas analysis apparatus of claim 1 , wherein claim 1 , in the seeker measurement path claim 1 , there is ...

Coupled Analytical Instruments for Dual Mode FTIR/GC-FTIR

A system and method are disclosed for analyzing samples, which includes a spectrometry system for detecting components of a sample; a gas chromatography column for separating the components of a sample; a first sample unit for receiving a first sample from a sample source; and a second sample unit for receiving a second sample from a sample source. Each sample loop unit allows independent processing of samples in preparation for analysis.

SYSTEM FOR DETECTING LIQUID ANALYTES

A sample cartridge for a liquid chromatography device includes a microfluidic chip. A collector in the microfluidic chip includes a collector flow channel and a first window for acquisition of spectral data from a sample in the collector flow channel. 1. A sample cartridge for a liquid chromatography device comprising:a microfluidic chip; anda collector formed in the microfluidic chip comprising a collector flow channel and a first window for acquisition of spectral data from a sample in the collector flow channel.2. The sample cartridge of claim 1 , further comprising:a case enclosing the sample cartridge, the case including a port, the port aligned with the first window.3. The sample cartridge of claim 1 , wherein the microfluidic chip further comprises a separator column in fluid communication with the collector flow channel.4. The sample cartridge of claim 3 , wherein the separator column comprises a separator structure and a separator flow channel through the separator structure.5. The sample cartridge of claim 4 , wherein the separator structure comprises a plurality of three-triangular pillars claim 4 , the three-triangular pillars arranged in a plurality of rows to define the separator flow channel.6. The sample cartridge of claim 5 , wherein:in each row of three-triangular pillars in the separator structure, a spacing from a center of one three-triangular pillar to a center an adjacent three-triangular pillar is a distance w; anda subsequent row of three-triangular pillars is laterally offset from a preceding row of three-triangular pillars by a distance w/2.7. The sample cartridge of wherein at least some of the plurality of three-triangular pillars comprise a seven-sided structure.8. The sample cartridge of claim 1 , wherein the microfluidic chip further comprises a switching valve.9. The sample cartridge of claim 8 , wherein the switching valve is in fluid communication with the separator column in at least one position.10. The sample cartridge of claim ...

AUTOSAMPLER

An autosampler includes a flow path switching valve to which a sample push unit and an analysis flow path are connected, a sample loop connected to the flow path switching valve, a sampling needle connected to the flow path switching valve, a metering pump connected to the flow path switching valve, and a control unit for controlling operations of the flow path switching valve and the metering pump. The control unit includes an injection volume addition unit for adding up injection volume of a same sample component injected into the sample loop. 1. An autosampler comprising:a flow path switching valve to which a sample push unit and an analysis flow path are connected;a sample loop connected to the flow path switching valve;a sampling needle connected to the flow path switching valve;a metering pump connected to the flow path switching valve; anda control unit for controlling operations of the flow path switching valve and the metering pump,wherein the control unit includesan injection/delivery control unit for switching the flow path switching valve so that the flow path switching valve configures a flow path for sample push for injecting a sample in a sample vial from the sampling needle into the sample loop by the metering pump, or a flow path for delivery for delivering a sample injected into the sample loop to an analysis flow path by a solvent from the sample push unit, andan injection volume addition unit for adding up injection volume of a same sample component injected into the sample loop.2. The autosampler according to claim 1 ,wherein the injection volume addition unit is for adding up the injection volume based on an instruction for the metering pump at a time of the injection/delivery control unit injecting a sample in the sample vial from the sampling needle into the sample loop by the metering pump.3. The autosampler according to claim 2 ,wherein the control unit further includes an injectable volume calculation unit for calculating an injectable ...

Methods and Systems for Detection of Non-Volatile Solutes

A system for detection of non-volatile solutes dissolved in solution comprises: a spray emitter system configured to receive a flow of the solution and generate an aerosol comprising droplets thereof, the generated droplets comprising a restricted size range; a spray chamber configured to receive the aerosol and emit a modified aerosol comprising droplets having a diameter smaller than a predetermined value; a conduit configured to receive a flow of the modified aerosol and to evaporate the solvent so as to generate an aerosol comprising solid particles of the solutes; a charging chamber configured to receive the aerosol and impart electric charge to the solid particles; and a detector configured to measure a quantity of charge imparted to the solid particles, wherein the restricted size range is such that solid particles having diameters greater than 10 nm comprise a substantial portion of all particles received by the charging chamber. 1. A system for detection and measurement of non-volatile solutes dissolved in a liquid solvent , comprising:a spray emitter system configured to receive a flow of liquid solution comprising the solvent and dissolved solutes and to generate an aerosol comprising droplets of the liquid solution, wherein the generated droplets comprise a restricted size range;a spray chamber configured to receive the aerosol and to emit a modified aerosol consisting essentially of the carrier gas and liquid droplets having a diameter smaller than a predetermined value;a conduit configured to receive a flow of the modified aerosol from the spray chamber and to cause evaporation of the solvent from the received liquid droplets so as to generate a further modified aerosol consisting essentially of the carrier gas and solid particles of the non-volatile solutes;a charging chamber configured to receive the further modified aerosol from the conduit and to impart electric charge to the solid particles thereof; anda detector configured to receive the charged ...

AUTOMATED FLUID HANDLING SYSTEM

Automated fluid handling system comprising a housing and two or more fluid handling units arranged as interchangeable modular components with an external fluidics section and an internal non fluidics section, and wherein the housing comprises a liquid handling panel with two or more of component positions for receiving said interchangeable modular components such that the external fluidics section is separated from the non fluidics section by the liquid handling panel. 143-. (canceled)44. An automated liquid chromatography system comprising:a housing;a master control unit connected to a system bus; andthree or more fluid handling units arranged as interchangeable modular components comprising (i) an external fluidics section, (ii) an internal non-fluidics section including a bus connector for directly connecting the interchangeable modular component with the system bus, and (iii) a panel member arranged to separate the fluidics section from the non-fluidics section;wherein the housing comprises a liquid handling panel with at least four component receiving positions arranged in a two dimensional array and adapted to receive said interchangeable modular components such that, when inserted, the fluidics section is external to the housing and the non-fluidics section is internal to the housing;wherein each component receiving position includes a complementary connector for connecting the bus connector of the interchangeable modular component inserted therein to said system bus;wherein each interchangeable modular component includes a dedicated CPU unit allowing the interchangeable modular component to independently perform operations in response to instructions over the system bus;wherein the master control unit is arranged to automatically identify interchangeable modular components;wherein said housing is adapted to accommodate at least one pump, at least one sensor unit and at least two fluid control valves of different configurations, of which at least three of the ...

LIQUID CHROMATOGRAPHY ANALYZER SYSTEM WITH ON-LINE ANALYSIS OF ELUTING FRACTIONS

A chromatography analyzer system () for analyzing a sample () includes a MIR analyzer () for spectrally analyzing a sample fraction (A) while the sample fraction (A) is flowing in the MIR analyzer (). The MIR analyzer () includes (i) a MIR flow cell (C) that receives the flowing sample fraction (A), (ii) a MIR laser source (A) that directs a MIR beam (B) in a MIR wavelength range at the sample fraction (A) in the MIR flow cell (C), and (iii) a MIR detector (D) that receives light from the sample fraction (A) in the MIR flow cell (C) and generates MIR data of the sample fraction (A) for a portion of the MIR wavelength range. 1. A chromatography analyzer system for analyzing a first sample fraction , the chromatography analyzer system comprising:a first MIR analyzer for spectrally analyzing the first sample fraction while the first sample fraction is flowing in the first MIR analyzer, the first MIR analyzer including (i) a first MIR flow cell that receives the flowing first sample fraction, (ii) a first MIR laser source that directs a first MIR beam having a first center wavenumber that is rapidly changed over time in a first MIR wavelength range at the first sample fraction in the first MIR flow cell, and (iii) a first MIR detector that receives light from the first sample fraction in the first MIR flow cell and generates first MIR data of the first sample fraction for the first MIR wavelength range.2. The chromatography analyzer system of further comprising a second MIR analyzer for spectrally analyzing the first sample fraction while the first sample fraction is flowing in the second MIR analyzer claim 1 , the second MIR analyzer including (i) a second MIR flow cell that receives the flowing first sample fraction claim 1 , (ii) a second MIR laser source that directs a second MIR beam having a second center wavenumber that is rapidly changed over time in a second MIR wavelength range at the first sample fraction in the second MIR flow cell claim 1 , and (iii) a ...

SYSTEM AND METHOD FOR CONTROLLING FLUID FLOW WITHIN A LIQUID CHROMATOGRAPHY SYSTEM

The present disclosure relates to methodologies, systems, and apparatus for controlling fluid flow within a chromatography system. The chromatography system includes a mobile phase pump configured to pump a liquid mobile phase through a column and a restrictor positioned downstream of the column and upstream of a detector. The system also includes a valve configured to operate in at least two positions. In a first position, the valve is configured to direct the output of the column to bypass the valve and reach the detector, while in the second position the valve directs the output of the column to waste. 1. A method for controlling fluid flow within a liquid chromatography system , the method comprising:operating a fluid valve in fluid communication with a makeup pump, an output of a chromatography column, and a channel to waste in a first position configured to divert a makeup fluid through the fluid valve to join the output of the chromatography column;directing the output of the chromatography column, when the fluid valve is in the first position, to bypass the fluid valve and flow through a restrictor and to a detector, wherein the restrictor is located downstream of the column, downstream of the fluid valve, and upstream of the detector; andoperating the fluid valve in a second position configured to divert the makeup fluid through the fluid valve directly upstream of the restrictor in order to create a backflow at the restrictor and divert the output of the chromatography column to waste via the fluid valve.2. The method of claim 1 , wherein the makeup pump is configured to pump the makeup fluid at a rate greater than the maximum flow rate of the restrictor when the fluid valve is operating in the second position.3. The method of claim 1 , wherein the makeup pump is configured to pump the makeup fluid at a rate lower than the maximum flow rate of the restrictor when the valve is operating in the first position.4. The method of claim 1 , wherein the detector ...

SYSTEM FOR DETECTING LIQUID ANALYTES

A sample cartridge for a liquid chromatography device includes a microfluidic chip. A collector in the microfluidic chip includes a collector flow channel and a first window for acquisition of spectral data from a sample in the collector flow channel. 120.-. (canceled)21. A microfluidic chip for a liquid chromatography device comprising: a collector flow channel;', 'a first window for acquisition of spectral data from a sample in the collector flow channel; and', 'a reflector supported on a surface of the collector flow channel., 'a collector formed in the microfluidic chip comprising22. The microfluidic chip of claim 21 , wherein the reflector is a mirror.23. The microfluidic chip of claim 21 , wherein the first window is arranged on a first side of the collector and the reflector is arranged on a second side of the collector claim 21 , the second side opposite of the first side claim 21 , such that a portion of the collector flow channel is between the first window and the reflector.24. The microfluidic chip of claim 23 , wherein the reflector is arranged parallel to the first window.25. The microfluidic chip of claim 24 , wherein a direction of flow through the portion of the collector flow channel between the first window and the reflector is parallel to the first window and the reflector.26. The microfluidic chip of claim 24 , wherein a direction of flow through the portion of the collector flow channel between the first window and the reflector is perpendicular to the first window and the reflector.27. The microfluidic chip of claim 26 , wherein a cross section of the collector channel is z-shaped as viewed from a side of the collector.28. The microfluidic chip of claim 21 , wherein the microfluidic chip further comprises a separator column in fluid communication with the collector flow channel.29. The microfluidic chip of claim 28 , wherein the microfluidic chip further comprises a switching valve in fluid communication with the separator column in at least ...

Chromatography Column Comprising an Internal Bracing

The invention discloses a bioprocess chromatography column comprising: a) a bed chamber delimited by at least one side wall, a first bed support screen and a second bed support screen; b) a first end wall, secured to or integral with the side wall(s), with a first port fluidically connected via a first distributor to the first bed support screen; c) a second end wall, secured to or integral with the side wall(s), with a second port fluidically connected via a second distributor to the second bed support screen; d) a packing port in a wall; and e) an internal bracing, secured to, or integral with, at least one of the end walls and extending into the bed chamber. 1. A chromatography column for bioprocess separations , said column comprising:a) a bed chamber delimited by at least one side wall, a first bed support screen and a second bed support screen;b) a first end wall, secured to or integral with said at least one side wall, with a first port fluidically connected via a first distributor to said first bed support screen;c) a second end wall, secured to or integral with said at least one side wall, with a second port fluidically connected via a second distributor to said second bed support screen;d) a packing port in one of said at least one side wall and first and second end walls; ande) an internal bracing secured to, or integral with, at least one of said first and second end walls and extending into said bed chamber.2. The chromatography column of claim 1 , wherein at least part of said internal bracing spans the entire axial height of the bed chamber claim 1 , being secured to claim 1 , or integral with claim 1 , both of the first and second end walls.3. The chromatography column of claim 1 , wherein said internal bracing comprises one or more rods claim 1 , each having a first rod end secured to claim 1 , or integral with claim 1 , said first end wall and a second rod end secured to claim 1 , or integral with claim 1 , said second end wall.4. The ...

Method And Apparatus For Automatic Chromatography Of Thin-Layer Plates

A method for the automatic chromatography of thin-layer plates for thin-layer chromatography with a development chamber in which a thin-layer plate D is completely enclosed, sealed-off and isolated from the external environment. In the development chamber, a front space containing an inner atmosphere is located on the front face of the separation layer of the thin-layer plate. The depth of the front space is about 2 mm and a maximum 3 mm. An inlet is provided at one end of the front space and an outlet is provided at the other end of the front space. During the chromatographic development, a stream of gas of particular composition determined by the user is created throughout the entire front space, the entire inner atmosphere being set in motion, without stagnant or stationary gas phase. 1. A method for the automatic chromatography of thin-layer plates for thin-layer chromatography with a development chamber in which a thin-layer plate is completely enclosed , sealed-off and isolated from the external environment , wherein a front space containing an inner atmosphere is located in the development chamber on a front face of a separation layer of the thin-layer plate , comprising the following steps:a) activating by adjustment of the moisture of the separation layer of the thin-layer plate;b) preconditioning by saturation of the inner atmosphere with a specific gas phase composition and defined loading of the separation layer of the thin-layer plate with at least one substance; andc) providing chromatographic development, wherein during at least one of steps a), b) or c), a stream of gas having a particular composition determined by a user is generated throughout the entire front space, and the entire inner atmosphere is set in motion, without stagnant or a stationary gas phase.2. The method according to claim 1 , wherein:the stream of gas drives the inner atmosphere already in the front space frontally out of the development chamber, without turbulences and without ...

Method And Apparatus For Automatic Chromatography Of Thin-Layer Plates

A method for the automatic chromatography of thin-layer plates for thin-layer chromatography with a development chamber 1 in which a thin-layer plate D is completely enclosed, sealed-off and isolated from the external environment. In the development chamber, a front space containing an inner atmosphere is located on the front face of the separation layer of the thin-layer plate. The depth of the front space is about 2 mm and a maximum 3 mm. An inlet is provided at one end of the front space and an outlet is provided at the other end of the front space. During the chromatographic development, a stream of gas of particular composition determined by the user is created throughout the entire front space, the entire inner atmosphere being set in motion, without stagnant or stationary gas phase.

SAMPLE INJECTION DEVICE

The present invention relates to an improvement in a technique of a large-volume sample injection in high-performance liquid chromatography or supercritical fluid chromatography. 2. The sample injection device according to claim 1 , wherein the first channel switching part comprises a stator part having six through holes at vertex positions of a regular hexagon claim 1 , and a rotor part having three channel grooves.3. The sample injection device according to claim 1 , wherein the sample injection device is used for sample injection in high-performance liquid chromatography or supercritical fluid chromatography.4. The sample injection device according to claim 2 , wherein the sample injection device is used for sample injection in high-performance liquid chromatography or supercritical fluid chromatography.5. A channel switching valve that can be used in a sample injection device for chromatography claim 2 , whereinthe channel switching valve has a two-position switching function capable of switching into two positions,the channel switching valve comprises a stator part that has six through holes at vertex positions of a regular octagon, and a rotor part that rotates in contact with the stator part to switch a channel,the rotor part has three channel grooves, and two of the through holes and one of the channel grooves are disposed to face and be in close contact with each other to form a channel,the six through holes are configured of: a first through hole (g) connected to a sample suction tube that is a channel for sucking a sample from a sample container; a second through hole (h) connected to a first channel switching valve that is used to form a liquid transfer channel for flowing a mobile-phase solvent; a third through hole (i) connected to a decompression part that has a sample loop pressure-releasing function; a fourth through hole (j) connected to the first channel switching valve; a fifth through hole (k) connected to a syringe that sucks the sample from ...

LIQUID CHROMATOGRAPHY ANALYTE REACTION AND ANALYSIS SYSTEM

In the present system and method, a conduit from a LC device continuously transports solvent, buffers, and analytes to the inlet of a solvent removal and analyte conversion device which evaporates the solvents, leaving non-volatile analytes for detection. The device comprises a rotating disk. The liquid chromatograph device can be any device using liquid chromatography to separate molecules. The solvents in the LC effluent can include, but are not limited to, water, methanol, acetonitrile, tetrahydrofuran, and acetone. After removal of the volatile components, the non-volatile analytes are converted with a concentrated energy source so that they may be detectable.

Fluid flow control system for simulated moving bed processes

System for diversion of a liquid chromatography eluant

自动化流体处理系统

一种自动化流体处理系统包括壳体(20)和两个或更多个流体处理单元(26)，该两个或更多个流体处理单元(26)布置成具有外部流控区段(30)和内部非流控区段(32)的可互换的模块式构件，以及其中，壳体(20)包括具有用于接收所述可互换的模块式构件(26)的构件位置中的两个或更多个的液体处理面板(22)，使得外部流控区段(30)与非流控区段(32)被液体处理面板(22)分开。

Predicting properties of well bore treatment fluids

Methods and systems for predicting properties of well bore treatment fluids are disclosed. An embodiment includes a method of predicting fluid properties comprising: determining an operational window for a well bore fluid system; collecting data at vertices of the operational window; and developing a model comprising predicted properties for a plurality of data points within the operational window, wherein developing the model uses Barycentric interpolation.

Predicting properties of well bore treatment fluids

Methods and systems for predicting properties of well bore treatment fluids are disclosed. An embodiment includes a method of predicting fluid properties comprising: determining an operational window for a well bore fluid system; collecting data at vertices of the operational window; and developing a model comprising predicted properties for a plurality of data points within the operational window, wherein developing the model uses Barycentric interpolation.

Predicting properties of well bore treatment fluids

Methods and systems for predicting properties of well bore treatment fluids are disclosed. An embodiment includes a method of predicting fluid properties comprising: determining an operational window for a well bore fluid system; collecting data at vertices of the operational window; and developing a model comprising predicted properties for a plurality of data points within the operational window, wherein developing the model uses Barycentric interpolation.

Gas chromatograph

(57)【要約】本公報は電子出願前の出願データであるた め要約のデータは記録されません。

Chromatography system

A controlled charge chromatography column for the purification of a fluid-containing material, which column comprises a chromatographic column having an inlet for the introduction of a fluid to be purified and an outlet for the discharge of the purified fluid, and one or more concentrated materials and a flow-through capacitor disposed within the column between the inlet and the outlet, the flow-through capacitor means comprising a plurality of spirally wound or stacked washer layers to include a first electrically conductive backing layer, such as of graphite, and a first high surface area conductive layer secured to the backing layer, such as composed of porous carbon fibers and a non-conductive, porous spacer layer to electrically insulate the backing and conductive layer and to permit the flow of material therethrough, the flow-through capacitor to be connected to a DC power source to charge the respective conductive layers with different polarities whereby a fluid containing material through the column is purified by the electrically conductive stationary phase and the retention thereof onto the high surface area layer and permitting for example the purification of solutions of liquids, such as salt, and providing for the recovery of a purified liquid.

Flow-through capacitor

A controlled charge chromatography column for the purification of a fluid containing materials, which column comprises a chromatographic column having an inlet for the introduction of a fluid to be purified and an outlet for the discharge of the purified fluid, and one or more concentrated materials and a flow-through capacitor disposed within the column between the inlet and the outlet, the flow-through capacitor means comprising a plurality of spirally wound, spaced apart layers to include a first electrically conductive backing layer, such as of graphite, and a first high surface area conductive layer secured to the backing layer, such as composed of porous carbon fibers and a non-conductive, porous spacer layer to electrically insulate the backing and conductive layer and to permit the flow of material therethrough, the flow-through capacitor to be connected to a DC power source to charge the respective conductive layers with different polarities whereby a fluid containing material through the column is purified by the electrically conductive stationary phase and the retention thereof onto the high surface area layer and permitting for example the purification of solutions of liquids, such as salt, and providing for the recovery of a purified liquid.

Flow-through capacitor

A flow-through capacitor and a controlled charge chromatography column system using the capacitor for the purification of a fluid-containing material, which column comprises an inlet for a fluid to be purified and an outlet for the discharge of the purified fluid, and a flow-through capacitor disposed within the column. The flow-through capacitor comprises a plurality of spirally-wound, stacked washer or rods to include a first electrically conductive backing layer, such as of graphite, and a first high surface area conductive layer secured to one side of the backing layer, such as carbon fibers, and a second high surface area conductive layer secured to the opposite side of the backing layer, the high surface area material layers arranged to face each other and separated by a nonconductive, ion-permeable spacer layer to insulate electrically the backing and conductive layer. The system includes a DC power source to charge the respective conductive layers with different polarities whereby a fluid-containing material passing through the column is purified by the electrically conductive, high surface area stationary phase and the retention thereof onto the high surface area layer and permitting, for example, the purification of aqueous solutions of liquids, such as salt, and providing for the recovery of a purified liquid.

Controlled charge chromatography system

A controlled charge chromatography column for the purification of a fluid-containing material, which column comprises a chromatographic column having an inlet for the introduction of a fluid to be purified and an outlet for the discharge of the purified fluid, and one or more concentrated materials and a flow-through capacitor disposed within the column between the inlet and outlet, the flow-through capacitor means comprising a plurality of spirally wound or stacked washer layers to include a first electrically conductive backing layer, such as of graphite, and a first high surface area conductive layer secured to the backing layer, such as composed of porous carbon fibers and a non-conductive, porous spacer layer to electrically insulate the backing and conductive layer and to permit the flow of material therethrough, the flow-through capacitor to be connected to a DC power source to charge the respective conductive layers with different polarities whereby a fluid containing material through the colum is purified by the electrically conductive stationary phase and the retention thereof onto the high surface area layer and permitting for example the purification of solutions of liquids, such as salt, and providing for the recovery of a purified liquid.