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Небесная энциклопедия

Космические корабли и станции, автоматические КА и методы их проектирования, бортовые комплексы управления, системы и средства жизнеобеспечения, особенности технологии производства ракетно-космических систем

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Мониторинг СМИ

Мониторинг СМИ и социальных сетей. Сканирование интернета, новостных сайтов, специализированных контентных площадок на базе мессенджеров. Гибкие настройки фильтров и первоначальных источников.

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Форма поиска

Поддерживает ввод нескольких поисковых фраз (по одной на строку). При поиске обеспечивает поддержку морфологии русского и английского языка
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Применить Всего найдено 2643. Отображено 100.
20-12-2012 дата публикации

Guided wave cutoff spectroscopy using a cylindrical measurement cell

Номер: US20120319702A1
Автор: Darrel Thomas Butler, Tom Lee Erb
Принадлежит: Thermo Fisher Scientific Inc

A cylindrical waveguide ( 1 ) for analyzing a flowing material ( 18 ) using guided microwave spectroscopy (GMS). Spaced apart plates ( 2, 5 ) each define a plane within the waveguide housing ( 3 ) that is parallel to the direction ( 47 ) of material flow through the waveguide. Two opposed frames ( 7, 19 ) each surround an aperture ( 6 ) that permits access to the region between the waveguide plates ( 2, 5 ). A microwave probe assembly ( 81 ) is mounted at each frame ( 7, 19 ) to permit the radiation and reception of electromagnetic waves within the housing ( 1 ). A temperature probe ( 51 ) is inserted into the interior of the housing ( 1 ) via fitting ( 13 ). A y-shaped assembly ( 89 ) can be used to divide the flow into two separate paths including a diverter vane ( 93 ) that permits flow to be equalized within the two separate flow paths.

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Номер записи: 1
08-08-2013 дата публикации

ANALYTICAL METHOD AND TITRATION DEVICE

Номер: US20130203171A1
Автор: Sportsman John Richard
Принадлежит: SPORTSMAN CONSULTING LLC

A device and reagents to be used in conjunction therewith for the determination of the level of sulfite or related species, including sulfur dioxide (SO), in red or white wine, musts, beer, juices, water, industrial process streams and other opaque media is described. The device provides electrodes and circuits designed for amperometric detection of the titration endpoint and for indicating by sight, sound or touch when the endpoint has been reached. The reagents allow quantitative volumetric determination of the iodometric endpoint when used with the device. The sulfite or related species levels are related directly to the volume of the quantitative reagents used. 1. A method of determining sulfite or related species concentration in an opaque solution comprising:placing a measured amount of the opaque solution into a container,contacting the measured opaque solution with an electrode comprising two poles, the electrode being electrically connected to a device for measuring current between the two poles,adding a substance to make the measured opaque solution acidic,adding iodide to make the measured opaque solution have an iodide concentration of greater than about 1 millimolar,titrating the measured opaque solution with a standardized solution of an oxidant to an endpoint determined by a current between the poles above a predetermined threshold, wherein the device indicates the endpoint has been reached.2. The method of wherein the opaque solution is wine claim 1 , must claim 1 , beer claim 1 , wort or juice.3. The method of further comprising:calculating the concentration of sulfite or related species in the opaque solution.4. (canceled)5. The method of wherein the minimum spacing of the portion of the poles in contact with the measured opaque solution is less than about 1 cm.6. The method of wherein the device impresses a voltage between the poles of less than about 0.5 V.7. The method of wherein the device impresses a voltage between the poles of about 0.1 V.8. ...

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Номер записи: 2
14-11-2013 дата публикации

Deformable transportable bioreactor chamber

Номер: US20130302897A1
Автор: Blaise Porter, Kent Vilendrer
Принадлежит: ILLINOIS TOOL WORKS INC

An apparatus and method is described for seeding and culturing cells on a sample. The apparatus includes a chamber in which the volume of the chamber may be adjusted without compromising the seal or sterility of the chamber. The apparatus enables the seeding of cells in a reduced volume and culturing of cells in an increased volume. Further, the apparatus enables application of forces, strains and torques to a sample during seeding, culturing or transportation of the sample.

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Номер записи: 3
09-01-2014 дата публикации

METHOD FOR COUNTING SUBJECT MATTERS TO BE COUNTED IN CONTAINER

Номер: US20140011186A1
Автор: Ishizaki Yoichi, Ota Kyohei, Suenaga Ryo, Tanaka Satoshi, Totani Takahiko
Принадлежит: TOYO SEIKAN KAISHA, LTD.

A method for counting counted targets disposed in a liquid enclosed in a container, includes adjusting a thickness of at least a part of the container; setting at least a part of the adjusted part as a measurement target region; and counting a number of counted targets in the measurement target region. 1. A method for counting counted targets disposed in a liquid enclosed in a container , comprising:adjusting a thickness of at least a part of the container;setting at least a part of the adjusted part as a measurement target region; andcounting a number of counted targets in the measurement target region.2. The method for counting counted targets disposed in a container according to claim 1 , further comprising claim 1 , prior to adjusting the thickness of at least the part of the container claim 1 , agitating the liquid in the container to equalize the counted targets in the liquid.3. The method for counting counted targets disposed in a container according to claim 1 , further comprising claim 1 , based on the counted number of the counted targets claim 1 , calculating a density of the counted targets in the liquid claim 1 , and/or calculating a number of counted targets throughout the liquid.4. The method for counting counted targets disposed in a container according to claim 1 , the counting step of the number of the counted targets comprises counting the number of the counted targets in a photographed image of the measurement target region.5. The method for counting counted targets disposed in a container according to claim 1 , further comprising reducing the thickness of at least the part of the container when the number of the counted targets in the measurement target region is equal to or more than a predetermine value claim 1 , and then counting the number of counted targets.6. The method for counting counted targets disposed in a container according to claim 5 , wherein the reducing step of the thickness of at least the part of the container comprises ...

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Номер записи: 4
23-01-2014 дата публикации

EXTRA-CAPILLARY FLUID CYCLING SYSTEM AND METHOD FOR A CELL CULTURE DEVICE

Номер: US20140024012A1
Автор: CREP MARTIN PEDER, PAGE DARRELL PAUL, WOJCIECHOWSKI ROBERT J.
Принадлежит:

An extra-capillary fluid cycling unit for maintaining and cycling fluid volumes in a cell culture chamber includes a housing and a first flexible reservoir extra-capillary fluid reservoir disposed in the housing. The extra-capillary fluid reservoir is in fluid communication with a cell culture chamber. A second flexible reservoir is also located in the housing, the second flexible reservoir being in fluid communication with a pressure source. A sensor plate is movably disposed in the housing between the extra-capillary reservoir and the second reservoir, wherein the second reservoir is pressurized to move the sensor plate in relation to the extra-capillary reservoir to cause fluid cycling and maintain fluid volumes in the cell growth chamber. 1. A fluid cycling unit for maintaining and cycling fluid volumes in a chamber comprising:a housing;a first flexible fluid reservoir disposed in the housing, said first fluid reservoir being in fluid communication with a chamber;a second flexible reservoir located in said housing, said second flexible reservoir being in fluid communication with a pressure source; anda sensor plate movably disposed in said housing between said first and second reservoirs, wherein said second reservoir is pressurized to move said sensor plate in relation to said first reservoir to cause fluid cycling and maintain fluid volumes in said chamber.2. The fluid cycling unit of claim 1 , further comprising a sensor indicator connected to said sensor plate claim 1 , wherein physical expansion and contraction of said first reservoir moves said sensor indicator within the housing.3. The fluid cycling unit of claim 2 , further comprising a sensor in communication with said pressure source claim 2 , wherein said sensor indicator drives said sensor to expand or reduce the pressure within said second reservoir.4. The fluid cycling unit of claim 1 , wherein said chamber comprises a hollow fiber bioreactor that provides cell space and media component exchange.5. ...

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Номер записи: 5
01-01-2015 дата публикации

BEVERAGE DIAGNOSTIC AND PRESERVATION DEVICES AND METHODS

Номер: US20150000371A1
Автор: Greene Russell W., Helffrich Jerome A., Kahute Trent J., Lipton Daniel B., Postell David J., Trettin David J., Vanecek Bob, WOOD JAMES H.
Принадлежит:

Beverage diagnostic and preservation devices and methods are described. In several exemplary embodiments, one or more of the devices are used to detect the freshness of, and/or preserve, wine in a container. The devices can receive inputs from one or more sensors when determining the freshness of wine in a container. 1. A method for determining freshness of wine , the method comprising:measuring a lower explosive limit of gas located within a headspace of a container; anddetermining freshness of wine in the container based on the lower explosive limit.2. The method of claim 1 , wherein the freshness of the wine is deemed fresh if the lower explosive limit of gas located in the headspace of the container is below about 5% claim 1 , 10% claim 1 , or 15%.3. The method of claim 1 , wherein the freshness of the wine is deemed not fresh if the lower explosive limit of gas located in the headspace of the container is above about 5% claim 1 , 10% claim 1 , or 15%.4. The method of claim 1 , wherein the lower explosive limit is measured with a photoionization detector claim 1 , semiconductor claim 1 , or metal oxide sensor.5. A method for determining freshness of wine claim 1 , the method comprising:{'sub': '2', 'measuring a concentration of SOin liquid wine in a container; and'}{'sub': '2', 'determining the freshness of the wine based on the concentration of SO.'}6. The method of claim 5 , wherein the freshness of the wine is deemed fresh if the concentration of SOin the liquid is greater than about 5 claim 5 , 10 claim 5 , 15 claim 5 , 20 claim 5 , or 25 ppm.7. The method of claim 5 , wherein the freshness of the wine is deemed not fresh if the concentration of SOin the liquid is less than about 5 claim 5 , 10 claim 5 , 15 claim 5 , 20 claim 5 , or 25 ppm.8. The method of claim 5 , further comprising:measuring a lower explosive limit of gas located within a headspace of the container; anddetermining freshness of wine in the container based on the lower explosive limit.9. ...

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Номер записи: 6
05-01-2017 дата публикации

DEVICE FOR SEPARATING CELLS IN FLUID

Номер: US20170002308A1
Автор: Liu Tao
Принадлежит:

The present disclosure provides a device for separating cells in fluid, comprising a first driving pump, a separation column, a detection column, a second driving pump, and several three-way valves and secondary driving pumps; one end of the separation column is connected with the first driving pump through a first three-way valve; the other end is connected with the second driving pump through a second three-way valve; the separation column includes at least five sub filtration columns in parallel; the sub filtration column comprises a fixing bracket and a track-etched membrane of polycarbonate or polyester material attached to the bottom of and the side surfaces all around the fixing bracket, wherein the pore diameter of the track-etched membrane is 5-25 μm. The device of the present disclosure not only provides a new method for accurately determining whether there are circulating tumor cells in the blood of the living animal and provides a new method for sorting and counting the circulating tumor cells in the blood of the living animal, but also provides a pioneering new method and new device for therapy of the tumor transfer and removal of the tumor cells inside the body, and has extremely high economic and social values. 1. A device for separating cells in fluid , comprising a first driving pump communicating with an sample injection port , a separation column , a detection column , a second driving pump for driving cells separated from the separation column to enter the detection column , and several three-way valves as well as secondary driving pumps ,wherein one end of the separation column is connected with the first driving pump through a first three-way valve, the other end of the separation column is connected with the second driving pump through a second three-way valve, the detection column is connected with a third channel port of the first three-way valve, and a third channel port of the second three-way valve is connected with a sample discharge ...

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Номер записи: 7
03-01-2019 дата публикации

PRESSURE-CONTROLLED APPARATUS FOR OPTIMAL CELLULAR GROWTH

Номер: US20190002816A1
Автор: Bontrager David, Douglas Ryan, Farrell Richard
Принадлежит:

Apparatus and associated methods relate to an optimizable cellular growth chamber, where growth conditions are optimized by adjusting gas pressures through a gas permeable membrane. In an illustrative example, an apparatus may provide a volume to contain growth medium and the cells under propagation, where the volume has limited but non-zero pneumatic communication with a pressure-controlled chamber via a gas-permeable membrane. Associated apparatus and methods are proposed to manage desired growth conditions via deliberate control of parameters, such as partial pressures, for example. 1. A pressure-controlled bio-growth chamber apparatus for optimal cellular growth comprising: at least one outer wall defining an interior chamber with an opening, the walls being configured to receive a lid to cover the opening to seal the chamber from ambient atmosphere; and,', 'a floor connected to the at least one wall to define a bottom boundary of the chamber;, 'an enclosure comprisinga control system housing exterior to and coupled to the enclosure;a pressure differential control mechanism operable to control a pressure differential with respect to the chamber; and,a circulation mechanism operably coupled to the enclosure and configured to introduce or aspirate fluids from the chamber,wherein said at least one outer wall is at least partially comprised of a gas permeable membrane having a first side and a second side and adapted to communicate gaseous elements from an interior of said enclosure to an exterior of said enclosure while substantially containing a liquid medium in the chamber and on the first side of the gas permeable membrane,wherein said at least one outer wall at least partially comprised of a gas permeable membrane defines a plenum chamber between the second side of the gas permeable membrane and an exterior of said enclosure,wherein the chamber is adapted to contain liquid media and biological matter supported on the gas permeable membrane, andwherein the ...

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Номер записи: 8
09-01-2020 дата публикации

SYSTEM FOR TREATING BIOMASS WITH A GAS

Номер: US20200010379A1
Автор: Hoff Svend
Принадлежит:

A system and a method for treating biomass with a gas includes at least one conduit having at least one biomass inlet and at least one biomass outlet, at least one gas inlet and at least one gas outlet. The system further includes a transport unit configured to move the biomass through the conduit from the at least one biomass inlet to the at least one biomass outlet thereby defining a biomass transport direction. The system is configured such that gas flowing from the at least one gas inlet to the at least one gas outlet crosses the biomass transport direction. 11. A system () for treating biomass , said system comprising:{'b': '2', 'claim-text': [{'b': 3', '4, 'at least one biomass inlet () and at least one biomass outlet (),'}, {'b': 5', '6, 'at least one gas inlet () and at least one gas outlet (), and'}], 'at least one conduit () comprising{'b': 7', '8', '3', '4, 'a transport unit (,) configured to move the biomass through the conduit(s) from the at least one biomass inlet () to the at least one biomass outlet () thereby defining a biomass transport direction,'}{'b': 5', '6, 'wherein the system is configured such that steam flowing from the at least one gas inlet () to the at least one gas outlet () crosses the biomass transport direction.'}2. The system according to claim 1 , wherein the at least one gas inlet is selected from the group of: air inlet claim 1 , steam inlet claim 1 , exhaust gas inlet claim 1 , and wherein the at least one gas outlet is correspondingly selected from the group of: air outlet claim 1 , steam outlet claim 1 , exhaust gas outlet claim 1 , preferably wherein the at least one gas inlet is a steam inlet claim 1 , and the at least one gas outlet is a steam outlet.32. The system according to any of the preceding claims claim 1 , wherein the at least one conduit () is configured to tolerate a negative pressure of at least 0.1 bar claim 1 , more preferably at least 0.2 or 0.3 bar.49. The system according to any of the preceding claims ...

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Номер записи: 9
11-01-2018 дата публикации

Quantitative Texture Measurement Apparatus and Method

Номер: US20180011069A1
Автор: BAI Ou, BOURG, FAGAN Scott, JR. Wilfred Marcellien, MICHEL-SANCHEZ Enrique, MIRZA Shahmeer Ali, RICHARDSON Scott G., SHAO Chen C.
Принадлежит:

A non-destructive measurement apparatus and method for quantitatively measuring texture of a food snack is disclosed. The apparatus includes a laser generating tool, an ultrasound excitation device, an acoustic capturing device, an ultrasound capturing device and a data processing unit. The laser generating tool and the ultrasound excitation tool direct energy towards a food snack placed on a surface and produce an acoustic signal and an ultrasound signal. The data processing unit further comprises a digital signal processing module that processes the received acoustic signal and ultrasound signal. A statistical processing module further filters the acoustic signal from the data processing unit and generates a quantitative acoustic model for texture attributes such as hardness and fracturability. The quantitative model is correlated with a qualitative texture measurement from a descriptive expert panel. Texture of food snacks are quantitatively measured with the quantitative acoustic model. 1. An apparatus for quantitative non-destructive texture attribute measurement of a food product comprising:a housing;a laser generator attached to said housing;an acoustic capturing device proximally located to said housing;an ultrasound excitation device proximally located to said housing;an ultrasound capturing device proximally positioned with respect to said acoustic capturing device;a data processing unit in communication with at least said acoustic capturing device and at least said ultrasound capturing device;wherein a laser from said laser generator is directed to strike said food product, thereby producing an acoustic signal to be detected by said acoustic capturing device; and an ultrasound wave from said ultrasound exciting device is directed to strike said food product, thereby producing an ultrasound signal to be detected by said ultrasound capturing device;wherein further said data processing unit is configured to quantitatively measure said texture attribute of ...

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Номер записи: 10
03-02-2022 дата публикации

Device for Distributing a Flow

Номер: US20220033750A1
Автор: Erickson Kerstin, Francois Tim, Gebauer Klaus, Lundin Andreas, Olovsson Bjorn
Принадлежит:

A flow distribution device for bioprocess systems, comprising: a flow distribution manifold () comprising: at least four fluid connection conduits (), wherein each fluid connection conduit () comprises a first end () for fluid connection and an opposite second end (), and wherein at least three of the fluid connection conduits comprise a membrane () and a valve seat (), which membrane () can be put in at least two different positions in relation to the valve seat () for allowing or preventing fluid flow between the first end () and the second end () of the fluid connection conduit (); and a central common compartment () to which the second ends () of each of the fluid connection conduits () are connected, whereby the first ends () of each of the fluid connection conduits () can be in fluid communication with the central common compartment () and wherein the fluid connection conduits () are entering the central common compartment () from at least three different directions; wherein said flow distribution device () further comprises at least three membrane actuation members () which are provided in connection with one membrane () of the flow distribution manifold () each, wherein each of said membrane actuation members () is configured for actuating the membrane () to be in at least two different positions in relation to the valve seat (), wherein a first position of the membrane () allows flow between the first end () and the second end () of the fluid connection conduit () and a second position of the membrane () prevents flow between the first end () and the second end () of the fluid connection conduit (). 2. The flow distribution device according to claim 1 , wherein at least five or at least six fluid connection conduits are provided in the flow distribution manifold.3. The flow distribution device according to claim 1 , wherein the fluid connection conduits are entering the central common compartment from at least four or at least five different directions.4. ...

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Номер записи: 11
15-01-2015 дата публикации

CELL CULTURE SYSTEMS

Номер: US20150017711A1
Автор: Bennett Scott Matthew, Kenney David Alan, Martin Gregory Roger, Tanner Allison Jean
Принадлежит:

An integrated cell culture system may include one or more cell culture vessels, manipulation apparatus, pumping apparatus, cell release apparatus, monitoring apparatus, and a control apparatus. The control apparatus may be used to monitor and control the system to facilitate effective cell culturing. The cell release apparatus may be used to release a plurality of cells adhered to the cell culture surfaces of the cell culture vessels. 1. A cell culture system comprising:at least one cell culture vessel configured to culture cells using a plurality of parallel cell culture surfaces, wherein the at least one cell culture vessel comprises at least one port configured to allow material to flow into and out of the at least one cell culture vessel;manipulation apparatus configured to rotate the at least one cell culture vessel about a first rotation axis and about a second rotation axis, wherein the first rotation axis is perpendicular to the second rotation axis, wherein each of the first rotation axis and the second rotation axis are parallel a ground surface;pumping apparatus fluidly coupled to the at least one port of the at least one cell culture vessel and configured to pump material into and out of the at least one cell culture vessel through the at least one port;monitoring apparatus configured to monitor one or more parameters of the at least one cell culture vessel, the manipulation apparatus, and the pumping apparatus; andcontrol apparatus operably coupled to the manipulation apparatus, the pumping apparatus, and the monitoring apparatus, wherein the control apparatus is configured to coordinate movement of the at least one cell culture vessel using the manipulation apparatus with the pumping of material into and out of the at least one culture vessel using the pumping apparatus.2. The system of claim 1 , wherein the control apparatus is further configured to:move, using the manipulation apparatus, the at least one cell culture vessel into at least one fill ...

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Номер записи: 12
19-01-2017 дата публикации

High Pressure Bioreactor

Номер: US20170015972A1
Автор: Foustoukos Dionysis Ioannis
Принадлежит: Carnegie Institute of Washington

The present invention relates generally to an integrated system, apparatus and method that allows for the continuous culturing of microorganisms under high pressure conditions and at a wide range of temperatures. More specifically, the system is configured to be gas tight and operate under aerobic or anaerobic conditions. The system is also configured to permit periodic sampling of the incubated organisms under such conditions with minimal physical/chemical disturbance inside the reactor and minimal impacts of shear forces on the collected biomass. 1. A method to allow for the continuous culturing of microorganisms under high pressure , the method comprising:a. providing a bioreactor capable of withstanding pressures of at least 150 MPa;b. pre-enriching media solution with dissolved gases in a reservoir;c. filling the bioreactor with growth media at high pressure and at the optimal temperature for growth;d. inoculating the bioreactor with culture;e. operating the bioreactor in the batch mode while the microbial community grows in density to a desired value;f. operating the bioreactor in a continuous mode by adding a continuous flow of media;g. increasing the pressure up to at least about 40 MPa; andh. monitoring the growth by sampling the reactor without affecting the hydrostatic pressure of the microbial community in bioreactor.2. The method of wherein the reactor is sampled using at least three valves arranged in series.3. The method of wherein the reservoir can withstand at least 60 psi of headspace partial pressure.4. The method of wherein the dissolved gases comprise one or more of H claim 1 , N claim 1 , COand O.5. The method of wherein the media solution is pre-heated prior to entering the bioreactor.6. The method of wherein the bioreactor is operated under aerobic or anaerobic conditions.7. The method of wherein sampling does not cause cell lysis.8. The method of wherein the pressure in the bioreactor is about 150 M Pa.9. A high pressure bioreactor for the ...

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Номер записи: 13
03-02-2022 дата публикации

Method for Determining a Weld Integrity Test Result

Номер: US20220034748A1
Автор: Castan Andreas, Gebauer Klaus, Hindupur Sameera Simha, Puranik Swapnil, Ramakrishna Manoj Kumar
Принадлежит:

The present invention relates to a computer implemented method for determining a weld integrity test result performed by a system configured to aseptically transferring cells to a container (BR), the method comprising welding () a vial to a connector to obtain a fluid-tight seal between the vial (V) and a connector (C), the connector being provided with a pair of conduits each having one end protruding through the connector and into the vial, sealing () an opposite end of one of the conduits, generating () a fluid pressure different to an ambient fluid pressure at an opposite end of the other conduit, measuring () a fluid pressure within at least one of the conduits, determining () a fluid pressure change based on the ambient pressure and the measured fluid pressure, determining () the weld integrity test result as pass or as fail based on the fluid pressure change. 1. A computer implemented method for determining a weld integrity test result performed by a system configured to aseptically transferring cells to a container , the method comprising:welding a vial to a connector to obtain a fluid-tight seal between the vial and a connector, the connector being provided with a pair of conduits each having one end protruding through the connector and into the vial,sealing opposite end of one of the conduits,generating a fluid pressure different to an ambient fluid pressure at an opposite end of the other conduit,measuring a fluid pressure within at least one of the conduits,determining a fluid pressure change based on the ambient pressure and the measured fluid pressure, anddetermining the weld integrity test result as pass or as fail based on the fluid pressure change.2. The method of claim 1 , wherein the weld integrity test result is determined as pass if the fluid pressure change is equal to or above a threshold or determining the test result as fail if the pressure is below the threshold.3. The method of claim 1 , wherein the method further comprises:sealing the ...

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Номер записи: 14
03-02-2022 дата публикации

FERMENTATION MONITORING SYSTEM

Номер: US20220034760A1
Автор: Botas Bilro Lúcia Maria, Estima da Costa Pedro Miguel, Gonçalves Fábio Patrício Domingues, Nunes Nogueira Rogério, Ventura de Sousa e Carvalho Pereira Ricardo José
Принадлежит:

A method and system for monitoring wine fermentation is provided. The system comprises a sensor conduit and a sensor enclosure. The sensor enclosure comprises a leveling indicator that indicates if the sensor conduit is vertical. 120-. (canceled)21. A system for monitoring , wherein the system comprises:a conduit;at least three legs; and the enclosure comprises a level configured to indicate when the conduit is vertical,', 'the at least three legs enable the conduit to be vertically positioned for monitoring, and', 'each of the at least three legs is individually adjustable in height., 'an enclosure operably coupled to the conduit and the at least three legs, wherein22. The system according to claim 21 , wherein the level comprises a bubble level.23. The system according to claim 21 , wherein the system is operable to monitor wine fermentation.24. The system according to claim 21 , wherein the system is operable to monitor a vat.25. The system according to claim 21 , wherein a filter is coupled to the enclosure.26. The system according to claim 21 , wherein an antenna is coupled to the enclosure.27. The system according to claim 21 , wherein the system is operable to monitor wine fermentation in a barrel.28. The system according to claim 21 , wherein the conduit extends through a bung.29. The system according to claim 28 , wherein a size of the bung is selected according to a bung hole of a barrel claim 28 , and wherein the hung is interchangeable with bungs of other diameters.30. The system according to claim 28 , wherein the bung is made of a material that degrades over time claim 28 , and wherein a degraded bung is interchangeable with a new bung.31. A method for monitoring claim 28 , wherein the method comprises:inserting a conduit into a container, wherein the conduit is operably coupled to an enclosure; and the level indicates when the conduit is vertical,', 'the enclosure is coupled to a plurality of legs, and', 'positioning the enclosure comprises adjusting ...

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Номер записи: 15
21-01-2016 дата публикации

System and Method for Non-Invasively and Non-Destructively Authenticating Bottled Beverages

Номер: US20160018375A1
Автор: FAHRNI Simon M., FULLER Benjamin T.
Принадлежит:

In one embodiment, authentication of a beverage is performed by forming an airtight seal with a bottle that contains the beverage, the bottle being sealed with a closure, applying a vacuum to the bottle to draw a sample from the closure that includes traces of the beverage, collecting the sample over time as the vacuum is applied to the closure, and performing testing on the collected sample.

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Номер записи: 16
17-01-2019 дата публикации

RADIATION SENSOR

Номер: US20190017088A1
Автор: Yoon Chang, Ziaie Babak
Принадлежит:

A radiation sensor, comprising a housing, a first chamber disposed in the housing and configured to contain a microorganism. A second chamber is disposed in the housing and configured to contain a fermentation material, the second chamber separated from the first chamber by a breakable separator. A breaking member is configured to break the breakable separator when pressed by a user. A flexible membrane is configured to flex when the microorganism ferments and thereby releases a gaseous byproduct. An electronic indicator is configured to relay information indicating the amount of fermentation, when the radiation sensor has been exposed to radiation less fermentation takes place resulting in a smaller volume of released gaseous byproduct. 1. A radiation sensor , comprising:a housing;a first chamber disposed in the housing and configured to contain a microorganism;a second chamber disposed in the housing and configured to contain a fermentation material, the second chamber separated from the first chamber by a breakable separator;a breaking member configured to break the breakable separator when pressed by a user;a flexible membrane configured to flex when the microorganism ferments and thereby releases a gaseous byproduct;an electronic indicator configured to relay information indicating the amount of fermentation, when the radiation sensor has been exposed to radiation less fermentation takes place resulting in a smaller volume of released gaseous byproduct.2. The radiation sensor of claim 1 , the microorganism is yeast.3. The radiation sensor of claim 1 , the fermentation material is glucose.4. The radiation sensor of claim 1 , the gaseous byproduct is CO.5. The radiation sensor of claim 1 , the electronic indicator includes a proximity switch claim 1 , a battery and a light emitting diode (LED) claim 1 , such that sufficient release of the gaseous byproduct results in closing of the proximity switch and thereby coupling of the battery to the LED and thereby ...

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Номер записи: 17
25-01-2018 дата публикации

SYSTEM AND METHOD FOR A BIOMIMETIC FLUID PROCESSING

Номер: US20180021780A1
Автор: Italiano Joseph, Mazutis Linas, Thon Jonathan N., Weitz David A.
Принадлежит:

A system and method are provided for harvesting target biological substances. The system includes a substrate and a first and second channel formed in the substrate. The channels longitudinally extending substantially parallel to each other. A series of gaps extend from the first channel to the second channel to create a fluid communication path passing between a series of columns with the columns being longitudinally separated by a predetermined separation distance. The system also includes a first source configured to selectively introduce into the first channel a first biological composition at a first channel flow rate and a second source configured to selectively introduce into the second channel a second biological composition at a second channel flow rate. The sources are configured to create a differential between the first and second channel flow rates to generate physiological shear rates along the second channel that are bounded within a predetermined range. 1. A biomimetic microfluidic system comprising:a substrate having a first portion and a second portion;a first channel formed in the first portion of the substrate, the first channel extending from a first input to a first output along a longitudinal dimension, the first channel configured to selectively receive at least one first biological composition at a first channel flow rate, the at least one first biological composition including a biological source material;a second channel formed in the second portion of the substrate, the second channel extending from a second input to a second output along the longitudinal dimension, the second channel configured to selectively receive at least one second biological composition at a second channel flow rate, wherein at least a portion of the first and second channels are substantially parallel;microchannels extending between the first channel and the second channel to create a fluid communication path between the first and second channels, wherein the ...

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Номер записи: 18
28-01-2016 дата публикации

Modular Bioreactor, Compliance Chamber for a Bioreactor, and Cell Seeding Apparatus

Номер: US20160024452A1
Автор: Delaney Christopher, III Richard, Pascal, Sierad Leslie, Simionescu Agneta, Simionescu Dan
Принадлежит:

Bioreactors and components of bioreactors are described as may be beneficially utilized in development and conditioning of cellular materials for study or implant. The bioreactors are modular and components of the bioreactors can be easily assembled with alternatives provided to develop specific, predetermined conditioning environments for cellular materials (e.g., implantable tissue). By selection of one of multiple alternative compliance chambers, a bioreactor can be utilized to condition tissue in a low pressure circuit (e.g., a pulmonary heart circuit), and by utilization of an alternative compliance chamber, the bioreactor can instead condition tissue in a high pressure circuit (e.g., an aortic heart circuit). 1. A compliance chamber for a bioreactor comprising a fluid conduit having a first end and a second , opposite end , the first end of the fluid conduit being removably locatable in fluid communication with a circulatory flow path through the bioreactor and the second end being maintained at atmospheric pressure when the first end is in fluid communication with the circulatory flow path , the first end of the fluid conduit being vertically lower than the second end when the first end is in fluid communication with the circulatory flow path such that upon addition of a liquid to the conduit a pressure head is established in the conduit.2. The compliance chamber for a bioreactor of claim 1 , the conduit having a cross-sectional area that is non-constant along the length of the conduit from the first end to the second end.3. The compliance chamber for a bioreactor of claim 2 , wherein the conduit has a circular cross section between the first end and the second end claim 2 , the diameter at the first end being from about 0.25 inches to about 2 inches and the diameter at the second end being from about 0 claim 2 ,1 inches to about 1.5 inches.4. The compliance chamber for a bioreactor of claim 1 , the length of the conduit between the first end and the second ...

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Номер записи: 19
04-02-2016 дата публикации

AERATION APPARATUS, AERATION METHOD AND CLEANING METHOD

Номер: US20160032233A1
Автор: CHEN CHIH-HSIANG, CHEN SHIH-CHANG, Chen Shih-Ming, CHIU CHUN-LUNG
Принадлежит:

An aeration apparatus, comprising: a gas-liquid mixing-circulating module, a gas supplying module, a controlling module and a cleaning unit, wherein the gas-liquid mixing-circulating module comprises: a first switch valve, connecting to a liquid outlet of a culture tank; a booster pump, connecting to the first switch valve; a micro-bubble producing device, having one end connecting to the booster pump and the other end connecting to a liquid inlet of the culture tank; a first proportional valve; a venturi tube, having a head, a tail and a branch, the other opening of the first proportional valve connecting to the head; and a second proportional valve, having an opening connecting to the tail, and the other opening connecting to the second tube, wherein, a fifth tube transfer gas to the venturi tube through the branch; wherein, the controlling module regulates the gas-liquid mixing-circulating module, the gas supplying module and the cleaning unit. 1. An aeration apparatus , for being installed in a microorganism culture tank to supply sterilized gas for microorganism cultivation and regulate the amount of gas supply , the aeration apparatus at least comprising: a first switch valve, connecting to a liquid outlet of the culture tank;', 'a booster pump, connecting to the first switch valve through a first tube;', 'at least one micro-bubble producing device, of which one end connects to the booster pump through a second tube, whereas of which the other end connects to a liquid inlet of the culture tank;', 'a first proportional valve, of which an opening connects to the first tube through a third tube;', 'a venturi tube, having a head, a tail and a branch, the other opening of the first proportional valve connecting to the head; and', 'a second proportional valve, of which an opening connects to the tail, whereas of which the other opening connects to the second tube through a fourth tube, wherein,', 'a fifth tube extends from the gas supplying module, the fifth tube ...

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Номер записи: 20
01-02-2018 дата публикации

SYSTEM, APPARATUS AND METHOD FOR THE PRODUCTION OF CELLS AND/OR CELL PRODUCTS

Номер: US20180030398A1
Автор: Castillo José
Принадлежит:

The present invention provides a system and a method for the production of cells and/or cell products. The system comprises at least one cell culture unit comprising at least one bioreactor for culturing cells, at least one technical control unit for controlling a cell growth parameters, said technical control unit is at least fluidly connected to the cell culture unit, and at least one air treatment unit for treating ambient air, said air treatment unit is fluidly connected to the cell culture unit. The system is characterized in that the system is autonomous and the bioreactor total volume is at most 1000 L. 2. System according to claim 1 , wherein the bioreactor total volume is at most 9001 claim 1 , preferably at most 800 L claim 1 , more preferably at most 700 L claim 1 , even more preferably at most 450 L claim 1 , most preferably at most 300 L claim 1 , even most preferably at most 250 L claim 1 , even most preferably at most 200 L.3. System according to claim 1 , wherein the bioreactor total volume is at least 1.5 L claim 1 , preferably at least 3 L claim 1 , more preferably at least 10 L claim 1 , even more preferably at least 30 L claim 1 , most preferably at least 50 L claim 1 , even most preferably at least 60 L.4. System according to claim 1 , wherein the technical control unit comprises at least one motion means for moving the bioreactor claim 1 , said motion means is mechanically and/or magnetically connected to the bioreactor and provides a movement selected from movements from right to left claim 1 , up to down claim 1 , rotating along a horizontal axis claim 1 , rotating along a vertical axis claim 1 , a rocking motion along a tilted or inclined horizontal axis of the bioreactor or any combination thereof.5. System according to claim 1 , wherein the technical control unit comprises at least one supply means connected to the bioreactor and to a culture medium reservoir for providing the bioreactor with culture medium claim 1 , said culture medium ...

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Номер записи: 21
17-02-2022 дата публикации

BIOMIMETIC CELL CULTURE APPARATUS AND CELL CULTURE SYSTEM COMPRISING THE SAME

Номер: US20220049205A1
Автор: Chung Kyung Sook, JUNG Cho Rok, KANG Hyun Mi, LIM Jung Hwa, NOH Kyung Hee, Son Mi Young, SON Myung Jin
Принадлежит: Korea Research Institute of Bioscience and Biotechnology

The present disclosure provides a biomimetic cell culture apparatus that mimics interactions among organs in a human body. The present disclosure includes a plurality of culture units for culturing cells, a conduit for connecting the plurality of culture units to each other to form a circulating path, a pump unit disposed on the conduit for forming a flow in culture medium such that the culture medium circulates through the plurality of culture units, and an agitating module for agitating the plurality of culture units. 1. A cell culture apparatus comprising:a plurality of culture units for culturing cells;a conduit for connecting the plurality of culture units to each other to form a circulating path;a pump unit disposed on the conduit for forming a flow in culture medium such that the culture medium circulates through the plurality of culture units; andan agitating module for agitating the plurality of culture units.2. The cell culture apparatus of claim 1 , wherein the agitating module moves or shakes the plurality of culture units in at least one direction.3. The cell culture apparatus of claim 1 , further comprising:a stage having one surface for mounting the plurality of culture units thereon and the other surface connected to the agitating module.4. The cell culture apparatus of claim 1 , wherein the culture unit includes:a main body connected to the conduit;a holder disposed in an inner space of the main body and having a plurality of insertion openings defined therein; andan insert to be respectively inserted into at least one of the insertion openings and supported by the holder, wherein the cells are arranged in the insert.5. The cell culture apparatus of claim 4 , wherein the holder further includes at least one side opening defined in a side wall thereof to face the conduit.6. The cell culture apparatus of claim 4 , wherein the holder further includes a guide opening connected to one side of each of the insertion openings.7. The cell culture apparatus ...

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Номер записи: 22
31-01-2019 дата публикации

SMART MICRO BIOREACTOR PLATFORM FOR HIGH THROUGHPUT MECHANICAL STIMULATION OF CARDIAC MICROTISSUE

Номер: US20190031991A1
Автор: PARSA Hesam, Vunjak-Novakovic Gordana
Принадлежит:

The present disclosure relates to a pneumatic microfluidic platform for high-throughput studies of cardiac hypertrophy that enables repetitive (hundreds of thousands of times) and robust (over several weeks) manipulation of cardiac μtissues. The platform is reusable for stable and reproducible mechanical stimulation of cardiac μtissues (each containing only 500 cells). Heterotypic and homotypic μtissues produced in the device were pneumatically loaded in a range of regimes, with real-time on-chip analysis of tissue phenotypes. Concentrated loading of the three-dimensional cardiac tissue faithfully recapitulated the pathology of volume overload seen in native heart tissue. Sustained volume overload of μtissues was sufficient to induce pathological cardiac remodeling associated with upregulation of the fetal gene program, in a dose-dependent manner. 1. A culture well for culturing microtissues comprising:a first pillar and a second pillar each extending vertically from the floor of the culture well, wherein the first and second pillars are separated by a distance and are aligned along a central axis traversing the width of the culture well, and wherein the first and second pillars each have a cross-sectional tear shape.2. The culture well of claim 1 , wherein the distance separating the first and second pillars is from 300 μm to about 700 μm.3. The culture well of claim 1 , wherein the distance separating the first and second pillars is from 400 μm to about 600 μm.4. The culture well of claim 1 , wherein the distance separating the first and second pillars is from 500 μm to about 600 μm.5. The culture well of claim 1 , wherein the depth of the culture well is from about 300 μm to about 500 μm.6. The culture well of claim 1 , wherein the width of the culture well is from about 1 claim 1 ,500 μm to about 2 claim 1 ,100 μm.7. The culture well of claim 1 , wherein the length of the culture well is from about 500 μm to about 700 μm.8. The culture well of claim 1 , wherein ...

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Номер записи: 23
30-01-2020 дата публикации

FLUID SUPPLY INTERFACE HAVING A SAFETY VALVE FOR A CELL CULTURE PLANT, AND THE USE THEREOF

Номер: US20200032195A1
Автор: Kuhne Oliver
Принадлежит: HAMILTON BONADUZ AG

A fluid supply interface includes at least one supply coupling configuration, at least one discharge coupling configuration, at least one user coupling configuration, and a fluid conduit that connects the supply, discharge, and user coupling configurations to one another. A supply valve is capable of having fluid flow through it or is blocked for flow through it. A discharge valve is capable of having fluid flow through it or is blocked for flow through it. The supply valve is preloaded into a blocking position that prevents flow, and opens by means of a sufficiently large pressure difference between the two sides of the supply valve, against the preload force, for flow through in a direction from the supply coupling configuration toward the fluid conduit. The discharge valve likewise being preloaded into a closed position that prevents flow through it. 1. A fluid supply interface for a cell culture system , the fluid supply interface comprising:at least one supply coupling configuration for coupling a respective supply fluid line onto the fluid supply interface;at least one discharge coupling configuration for coupling a respective discharge fluid line, different from the supply fluid line, onto the fluid supply interface;at least one user coupling configuration for coupling a respective user fluid line, different from the supply fluid line and the discharge fluid line, onto the fluid supply interface;a fluid conduit that connects the at least one supply coupling configuration, the at least one discharge coupling configuration, and the at least one user coupling configuration to one another;at least one supply valve by means of which, depending on its position, a supply coupling configuration is capable of having fluid flow through it or is blocked for flow through it;at least one discharge valve, different from the supply valve, by means of which, depending on its position, a discharge coupling configuration is capable of having fluid flow through it or is blocked ...

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Номер записи: 24
04-02-2021 дата публикации

MULTIPLE INCUBATOR CELL CULTURE SYSTEM WITH ATMOSPHERIC REGULATION OPERATED BY AN INTEGRATED CONTROL SYSTEM

Номер: US20210032587A1
Автор: ERNDT Nicholas George, FETH Brian, LIM James, PHI Janette Trang, RUTHERFORD Becky
Принадлежит:

Embodiments of a cell culture incubator system provided herein have two or more individual incubators and atmospheric regulation system configured to regulate atmospheric conditions within the environmental chamber of each individual incubator. The atmospheric regulation system has a single integrated controller system that controls atmospheric regulation of each of the individual incubators independently of the one or more other individual incubators. Atmospheric conditions within each of the individual incubators include at least the oxygen level and the total gas pressure, which are regulated independently of each other. 1. A cell culture incubator system comprising:two or more individual incubators, wherein each individual incubator comprises an environmental chamber; andan atmospheric regulation system configured to regulate atmospheric conditions within the environmental chamber of each individual incubator, the atmospheric conditions comprising an oxygen level and a total gas pressure,wherein the atmospheric regulation system is configured to regulate the oxygen level and the total gas pressure within each incubator independently of each other, andwherein the atmospheric regulation system comprises a single integrated controller system configured to command atmospheric regulation of each of the individual incubators independently of the one or more other individual incubators.2. The cell culture incubator system of claim 1 , wherein the integrated controller system comprises:a single master controller operably linked to each of the individual incubators; andtwo or more subcontrollers, each subcontroller dedicated, respectively, to one of the two or more individual incubators,wherein the master controller is configured to deliver atmospheric condition set point commands to each of the two or more subcontrollers, and wherein each subcontroller regulates the atmospheric regulation system of the individual incubator to which it is dedicated, in accordance with ...

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Номер записи: 25
07-02-2019 дата публикации

CULTURE MEDIUM STERILIZED FOR MICROALGAE HIGH DENSITY CULTURE, AND THE AIR COMPRESSION, AIR COOLING, CARBON DIOXIDE AUTOMATICALLY SUPPLIED, SEALED VERTICAL PHOTOBIOREACTOR, HARVESTING, DRYING APPARATUS AND CHARACTERIZED IN THAT TO PROVIDE A CARBON DIOXIDE BIOMASS CONVERSION FIXED, AIR AND WATER PURIFICATION METHOD USING THE SAME

Номер: US20190040347A1
Автор: KIM Youngnam, Park Young Woo
Принадлежит:

A microalgae culture broth producing system includes a device for culture broth sterilization using a micro bubble generator, an air compression and pressure equalization device for the injection of carbon dioxide and oxygen in the atmosphere into the culture broth. The system also includes an air chilling device to maintain suitable culture broth temperature when water temperature is too high, an automatic carbon dioxide supply device to promote photosynthesis, and a sealed vertical photobioreactor to block out pollutants and increase dissolved carbon dioxide and oxygen concentration. The system further includes a high-efficiency harvesting device using hollow fiber membranes, and a hot air drying device using the waste heat generated by air compression. 1. A microalgae culture broth producing system comprising:a device for culture broth sterilization using a micro bubble generator;an air compression and pressure equalization device for the injection of carbon dioxide and oxygen from the atmosphere into the culture broth;an air chilling device to maintain suitable culture broth temperature in response to a water temperature being higher than a predetermined maximum temperature;an automatic carbon dioxide supply device to promote photosynthesis;a sealed vertical photobioreactor configured to contain a culture medium inoculated with a microalgae, the vertical photobioreactor being configured to allow light into the culture medium, block out pollutants and increase dissolved carbon dioxide and oxygen concentration;a high-efficiency harvesting device using hollow fiber membranes; anda hot air drying device using the waste heat generated by air compression.2. The microalgae culture broth producing apparatus according to claim 1 , wherein the micro bubble generator sterilizes competing microalgae and microbes present in groundwater or treated water from sewage treatment plants using OH radicals.3. The microalgae culture broth producing apparatus according to claim 1 , ...

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Номер записи: 26
12-02-2015 дата публикации

SYSTEMS AND METHODS FOR EVALUATION OF WINE CHARACTERISTICS

Номер: US20150046471A1
Автор: Tompkins Michael J.
Принадлежит: VineSleuth, Inc.

A method comprises: receiving wine evaluations of wines from wine panelists, each wine evaluation including intensity values describing a plurality of wine characteristics for each of a set of wines, each wine evaluation generated by a wine panelist; generating a global intensity value from particular intensity values describing a particular wine characteristic of a particular wine, the particular intensity values being from the wine evaluations; comparing a selected intensity value generated by a selected wine panelist describing the particular wine characteristic for the particular wine against the global intensity value to determine an accuracy deviation; comparing the accuracy deviation against an accuracy deviation threshold to determine whether the selected intensity value is deemed inaccurate based on the comparison; updating the global intensity value for the particular wine characteristic for the particular wine based on the accuracy determination; and storing the updated global intensity value in a wine database. 1. A system comprising:a wine panelist interface module configured to receive wine evaluations of wines from wine panelists, each wine evaluation including intensity values describing a plurality of wine characteristics for each of a set of wines, each wine evaluation generated by a wine panelist;an accuracy management module configured to generate a global intensity value from particular intensity values describing a particular wine characteristic of a particular wine, the particular intensity values being from the wine evaluations, configured to compare a selected intensity value generated by a selected wine panelist describing the particular wine characteristic for the particular wine against the global intensity value to determine an accuracy deviation, and configured to compare the accuracy deviation against an accuracy deviation threshold to determine whether the selected intensity value is deemed inaccurate based on the comparison;a wine ...

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Номер записи: 27
15-02-2018 дата публикации

TISSUE PROCESSING APPARATUS AND METHOD FOR INFUSING BIOACTIVE AGENTS INTO TISSUE

Номер: US20180044638A1
Автор: Bhattacharyya Subhabrata, Kaes David R., Shimko Daniel A.
Принадлежит:

A tissue treatment apparatus and method for treating bone tissue has a controller, an enclosure, a reagent supply system, a draining configuration, and a gas relief valve is controlled by the controller. A bioactive agent may be applied through the reagent supply system. Optionally provided is a gas evacuation assembly, a gas supply unit, a thermal unit for heating or cooling reagents or gases, a sonication unit, any or each operated by the controller. The method provides for programming controller to effect a treatment procedure. In an embodiment of the present application the treatment procedure effects demineralization of the bone material. 1. An apparatus for processing a bone tissue sample , the apparatus comprising:a controller;an enclosure defining a reaction chamber for accepting the bone tissue sample;a reagent supply system configured to receive at least one reagent, the at least one reagent comprising at least one bioactive agent configured to contact the bone tissue sample, and to dispense the at least one reagent into the reaction chamber in response to the controller;a gas evacuation assembly communicated to the reaction chamber and configured to pump gas from the reaction chamber in response to the controller;a draining assembly communicated to the reaction chamber and configured to drain fluid from the reaction chamber in response to the controller; anda signal transmission system functionally interconnecting the controller with the reagent supply system, the gas evacuation assembly, and the draining assembly.2. An apparatus of claim 1 , further comprising a fluid level detector disposed to detect a fluid level in the reaction chamber and communicate the fluid level to the controller via the signal transmission system and the bone tissue sample comprises soft tissue.3. An apparatus of claim 1 , further comprising a gas pressure detector disposed to detect a gas pressure in the reaction chamber and communicate the gas pressure to the controller via ...

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Номер записи: 28
03-03-2022 дата публикации

ISOTOPIC MARKING AND IDENTIFICATION OF LIQUIDS

Номер: US20220065836A1
Автор: FAUVET Patrice, MIGEON Valérie
Принадлежит:

Method for isotopic identification, allowing, where appropriate, a liquid to be linked to a site of growth or harvest or a sub-product derived from a determined liquid, via analysis of concentration or of ratios of stable isotopes, and comparison to isotopic codes generated beforehand in a manner unique to a set of liquids issued from a site of growth, harvest or conversion. The invention also relates to a method that makes it possible to give a unique code to liquids of a site of growth, harvest or conversion, and to a computer making it possible to store the unique codes generated in memory, to generate unique codes for new liquids of a site of growth, harvest or conversion and to perform comparisons. 1. A method allowing a specific code of a place of origin to be applied to liquids , preferably wines or spirits , or to by-products of this liquid;method wherein the basic geographic signature (BGS) is provided of the liquid or by-product thereof, comprising:(i) the concentrations of at least 5, 6, 7, 8, 9, 10, 11, 12 or the entirety of the elements in group (3): Rb, Sr, B Li, Ca, Na, Mg, K, F, P, C1, As, Pb, Cd,(ii) the concentrations of at least 5, 10, 15, 20, 25, 30, or the entirety of the elements in group (2): Be, Al, V, Cr, Mn, Fe, Co, Ni, Cu, Zn, Ga, Ge, Se, Y, Zr, Nb, Mo, Rh, Pd, Ag, Sn, Sb, Te, I, Ba, Hf, Ta, W, Re, Ir, Hg, Ti, Si, and(iii) the concentrations of stable isotopes stables of at least 1, 2, 3, 4, 5, 6, 7, 8, 9 or 10 of the elements in this group (2);the method comprising the addition to this liquid having this BGS of a known quantity of at least 1 stable isotope of at least 1, 2 or 3 chemical elements of group (2), by means of which a liquid is obtained having a determined isotopic signature, with a modified isotope ratio compared with the BGS.2. The method according to claim 1 , wherein the BGS also comprises the concentrations of chemical elements of at least 2 claim 1 , 3 claim 1 , 4 or the 5 chemical elements in group (1): C claim 1 , O ...

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Номер записи: 29
05-03-2015 дата публикации

DEVICE FOR OPTICALLY DETERMINING THE CONCENTRATION OF ALCOHOL AND CARBOHYDRATES IN A LIQUID SAMPLE

Номер: US20150060674A1
Автор: Levels Geert Hubert Willem, Martynowicz Emile Thomas Martinus Johannes
Принадлежит:

Disclosed is a device for optically determining a concentration of alcohol and carbohydrates in a liquid sample. The device includes at least a first and a second light source arranged for exposing the liquid sample in a wavelength range between 750 nm and 1000 nm, a spectrometer arranged to determine a first and a second light intensity by measuring the light from the first and the second light source, a processing unit which is connected to the spectrometer and which is arranged to determine an absorption value of the liquid sample from a comparison of the first and the second light intensity with a reference value. In certain aspects, the device may further include a processing unit that calculates concentrations of alcohol and/or carbohydrates and at least two polarization filters. 1. A device for optically determining a concentration of alcohol and carbohydrates in a liquid sample , comprising:a first and a second light source arranged for exposing the liquid sample in a wavelength range between 750 nm and 1000 nm;a spectrometer arranged to determine a first and a second light intensity by measuring the light from the first and the second light source;a processing unit connected to the spectrometer and arranged to determine an absorption value of the liquid sample from a comparison of the first and the second light intensity with a reference value andtwo polarization filters for filtering the light from the first light source, wherein:the processing unit is further arranged to calculate the concentration of alcohol from the proportion of the absorption at a wavelength of less than 900 nm and the wavelength range between 900 nm and 920 nm, and to calculate the concentration of carbohydrates from the proportion of the absorption in the wavelength range between 750 nm and 900 nm and at a wavelength of 900 nm, andthe first polarization filter is positioned between the first light source and the liquid sample and the second polarization is positioned between the ...

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Номер записи: 30
21-02-2019 дата публикации

DISPOSABLE BIOASSAY CARTRIDGE AND METHOD OF PERFORMING MULTIPLE ASSAY STEPS AND FLUID TRANSFER WITHIN THE CARTRIDGE

Номер: US20190054460A1
Автор: ENGLERT DAVID, IZMAILOV ALEXANDRE, Smith Paul
Принадлежит:

The present disclosure provides a cartridge and method to move fluids within the cartridge that simplifies the design and removes the need for any internal valves or metering devices. The design is amenable to injection molded manufacturing lowering cost for large volume manufacturing. The design can be adapted to carry out both sample preparation and detection of biological substances including nucleic acids and proteins. 138-. (canceled)39. A porous substrate for detection of surface bound substances , comprising:a generally planar microporous substrate material having opposed surfaces and pores extending through a thickness of said substrate in which the pores are wider near one surface of the substrate compared to a width of the pores on the opposed surface thereby improving the collection efficiency of light emitted from optical probes bound to the interior surfaces of the widened pores.40. The porous substrate according to in which the pores are progressively wider near one surface of the substrate.41. The porous substrate according to in which the pores have a rectangular cross section.42. The porous substrate according to in which the pores have a square cross section.43. The porous substrate according to in which the pores have a circular cross section.44. The porous substrate according to in which the tapering is conical.45. The porous substrate according to in which the tapering is spherical.46. The porous substrate according to in which the tapering is parabolic.47. The porous substrate according to in which the pore dimension on the side opposite the side of the widened pores are substantially smaller thereby providing structural stability.48. The porous substrate according to which the pores are of uniform dimensions and morphology.49. The porous substrate according to further comprising reinforcement ribs to provide structural stability.50. The porous substrate according to in which the reinforcement ribs are an integral part of the substrate.51. The ...

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Номер записи: 31
21-02-2019 дата публикации

OBJECT-HOLDING DEVICE

Номер: US20190055507A1
Автор: ITO Saburo
Принадлежит: YAMAHA HATSUDOKI KABUSHIKI KAISHA

An object-holding device includes a container, a plate, and a dispersing mechanism. The container is configured to store a liquid, and includes an upper opening for charging an object into the stored liquid, and a bottom wall. The plate has an upper surface and a lower surface and is immersed into the liquid in a state in which the lower surface is spaced away from the bottom wall of the container, with this plate including: one or a plurality of holding portions arranged on the upper surface side and configured to carry the object; and a through hole passing through the holding portion from the upper surface to the lower surface. The dispersing mechanism is configured to form, in the through hole, a liquid flow which flows from the lower surface side toward the upper surface side to raise the object carried on the holding portion. 1. A plate having an upper surface and a lower surface , and configured to carry an object and immerse into a liquid in a state in which the lower surface faces and is spaced away from a bottom wall of a container configured to store the liquid , the plate comprising:at least one holding portion being a recess opened on the upper surface and configured to carry the object; anda through hole continuously arranged below the holding portion and passing through the plate from the upper surface to the lower surface, whereinthe holding portion includes a tubular portion having a constant opening area and an inclined portion continuously arranged below the tubular portion, the inclined portion having an opening area which decreases toward the through hole,the tubular portion defines a space in which the object with a desired size is housed,the inclined portion is a surface on which the object is held, anda size of the through hole is selected to prevent the desired size of the object from passing therethrough and allow a substance smaller than the desired size to pass therethrough.2. The plate according to claim 1 , whereinan upper edge portion ...

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Номер записи: 32
02-03-2017 дата публикации

CONTROLLING PRESSURE

Номер: US20170058257A1
Автор: Fernandez-Alcon Josa, Gomes Joshua, Hinojosa Christopher David, Levner Daniel, Sliz Josiah Daniel
Принадлежит:

A culture module is contemplated that allows the perfusion and optionally mechanical actuation of one or more microfluidic devices, such as organ-on-a-chip microfluidic devices comprising cells that mimic at least one function of an organ in the body. A method for pressure control is contemplated to allow the control of flow rate (while perfusing cells) despite limitations of common pressure regulators. The method for pressure control allows for perfusion of a microfluidic device, such as an organ on a chip microfluidic device comprising cells that mimic cells in an organ in the body, that is detachably linked with said assembly, so that fluid enters ports of the microfluidic device from a fluid reservoir, optionally without tubing, at a controllable flow rate. 1. A method of controlling pressure while perfusing cells , comprising: A) providing a) a plurality of microfluidic devices , each of said microfluidic devices comprising i) one or more microchannels comprising living cells and ii) one or more reservoirs comprising culture media , b) one or more pressure actuators , B) coupling said pressure actuators to at least one of the said reservoirs , the coupling adapted such that actuated pressure modulates the perfusion of at least some of said living cells , C) turning said one or more pressure actuators between two or more pressure setpoints , thereby controlling pressure while perfusing said cells.2. The method of claim 1 , wherein said pressure actuators are turned between two or more setpoints in a switching pattern.3. The method of claim 2 , wherein the switching pattern is selected such that the average value of pressure acting liquid in said one or more reservoirs corresponds to a desired value.4. The method of claim 3 , wherein the switching pattern is selected such that the average gas pressure is maintained below 1 kPa.5. The method of claim 1 , wherein each of the microfluidic device comprises a top channel claim 1 , a bottom channel claim 1 , and a ...

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Номер записи: 33
02-03-2017 дата публикации

Outgas Measurement Device

Номер: US20170059541A1
Автор: Howard Gregory
Принадлежит: TrenLot, Inc.

An apparatus is described to detect, measure or both, key compounds, such as sulfur dioxide (SO2) associated withoutgassed elemental compounds suggestive of the composition of one or more constituents in a liquid. 1. An outgas measurement device , comprising:a housing wherein the housing is hollow;a porous membrane; anda measurement sensor, wherein the measurement sensor is an electrochemical gas measurement sensor.2. The outgas measurement device of claim 1 , wherein the measurement sensor measures outgassed constituents in one or more barrels.3. The outgas measurement device of claim 2 , wherein the one or more barrels comprise one or more of a wine barrel claim 2 , a whisky barrel claim 2 , or a beer oak barrel.4. The outgas measurement device of claim 1 , wherein the measurement sensor measures outgassed constituents in one or more tanks.5. The outgas measurement device of claim 4 , wherein the one or more tanks comprise one or more of a wine tank claim 4 , or a whisky tank.6. The device of claim 1 , wherein the measurement sensor measures sulfur dioxide.7. The device of claim 1 , wherein the measurement sensor measures one or more outgasses compounds naturally occurring from the decomposition of wood barrel material.8. The device of claim 7 , wherein the one or more outgasses compounds comprise one or more hydrocarbon based gases.9. The device of claim 8 , wherein the one or more hydrocarbon based gases comprise Methane (CH4).10. The device of claim 1 , wherein the measurement sensor measures one or more of outgassed ethanol alcohol claim 1 , hydrocarbons claim 1 , and nitrogen dioxide. The present application for patent claims priority through the applicant's prior provisional patent application, entitled:A portion of the disclosure of this patent document contains or may contain material subject to copyright protection. The copyright owner has no objection to the photocopy reproduction of the patent document or the patent disclosure in exactly the form it ...

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Номер записи: 34
02-03-2017 дата публикации

SYSTEM AND METHOD FOR DETECTION OF A CONTAMINATED BEVERAGE

Номер: US20170059542A1
Автор: Abramson Michael T., MacDonald John C.
Принадлежит:

A system and method for a beverage container configured to test for a contaminated beverage in the beverage container comprises a base of the beverage container, an upper portion of the beverage container, and a sidewall with an inner surface and an outer surface that extends from the base to the upper portion, and a testing material. A portion of the beverage container is the testing material configured to visibly react when the portion of the beverage container is contacted with the contaminated beverage. 1. An apparatus configured to test a beverage , comprising:a straw, wherein the straw comprises a material; andan indicator chemically bonded to at least a portion of the material of the straw, wherein the indicator visibly reacts with a colorimetric response when the indicator is contacted with alcohol to indicate the alcohol is present in the beverage, wherein at least the portion of the material of the straw comprises the indicator, and wherein the visible reaction with the colorimetric response is shown on at least the portion of the material of the straw via the indicator chemically bonded to at least the portion of the material of the straw, wherein the indicator includes a colorimetric indicator, and wherein the indicator is chemically bonded to at least the portion of the material of the straw via at least one of an intramolecular bond and an intermolecular bond.2. The apparatus of wherein at least the portion of the material of the straw comprises a film claim 1 , and wherein the film comprises the chemically bonded indicator.3. The apparatus of wherein the visible reaction is shown on at least the portion of the material of the straw via the indicator chemically bonded to at least the portion of the material of the straw when a concentration threshold of the alcohol is present in the beverage.4. The apparatus of wherein the indicator is at least one of present on a surface of at least the portion of the material of the straw and within at least the ...

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Номер записи: 35
17-03-2022 дата публикации

METHOD FOR DETERMINING A KNEADING STATE OF A DOUGH, SYSTEM FOR MONITORING THE KNEADING STATE AND KNEADING MACHINE

Номер: US20220079170A1
Автор: Cheio De Oliveira Jose, FONTAINE Joran, LE BAIL Alain, REBILLARD Adrien, RIBETTE LANCELOT Eloïse
Принадлежит:

A method for determining a kneading state of a dough such as a cereal dough, wherein the dough being-is configured to be kneaded in a kneading machine, the kneading machine comprising a plurality of having sensors configured to measure representative quantities of the dough during a kneading cycle and at least one kneading tool. The method includes the following steps: 1. A method for determining a kneading state of a dough such as a cereal dough , the dough being configured to be kneaded in a kneading machine , the kneading machine comprising a plurality of sensors configured to measure representative quantities of the dough during a kneading cycle and at least one kneading tool , the method including the following steps:continuously collecting the measurements of the representative quantities of the dough during the kneading cycle,determining a kneading state based on a kneading state model defined as a function of the measurements of the representative quantities, the kneading state model being defined or adapted based on a learning phase, andchecking up the fulfillment of a kneading stop criterion based on the kneading state.2. The determination method according to claim 1 , comprising a step of stopping the kneading cycle of the dough if the kneading stop criterion is met.3. The determination method according to claim 1 , wherein the learning phase comprises at least one test cycle comprising the following steps:a. continuously collecting the measurements of the representative quantities during a kneading cycle,b. defining the kneading state model, andc. defining the kneading stop criterion.4. The determination method according to claim 1 , wherein the learning phase comprises a step of defining ranges of values of the representative quantities corresponding to the kneading stop criterion.5. The determination method according to claim 2 , wherein the learning phase comprises a step of checking up the dough allowing defining or adapting the kneading stop ...

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Номер записи: 36
28-02-2019 дата публикации

SOURCE-TRACING METHOD OF WINE PRODUCING AREA BASED ON MULTI-ELEMENTS AND STABLE

Номер: US20190064135A1
Автор: BIAN Xuehai, Chen Bo, JIN Baohui, Jin XiaoLei, Wu Hao, XIE Liqi, Yan Zhi, ZHAO Xu
Принадлежит:

The present application provides a method for tracing wine origin based on multielements and stable isotopes, comprising the steps of: 1). A method for tracing wine origin based on multielements and stable isotopes , characterized by comprising steps of:1) sample collection: collecting wine samples from a plurality of origins;{'sub': '3', '2) sample pre-processing: filtering each of the wine samples collected in Step 1) using a pore size of 0.22 μm aqueous filter membrane, and pouring 1.5 ml of a filtrate into an autosampler vial for carbon stable isotope ratio analysis of ethanol and glycerol; pouring 0.3 ml of the wine sample into a 12 ml glass tube with a stopper for analyzing oxygen stable isotopes in water; and pouring 0.5 ml of the wine sample into a 15 ml centrifuge tube, and adding 0.5 ml of concentrated HNO, performing digestion overnight, and diluting to a constant volume of 10 ml to be tested;'}3) sample analysis: performing carbon stable isotope analysis, oxygen stable isotope ratio analysis and elemental analysis, respectively;4) statistical modeling: utilizing information of the origins of the wine samples and analytical data of Step 3) to perform statistical modeling to obtain a origin discrimination model coefficient matrix and a corresponding predicted accuracy rate of the origin discrimination; and5) sample testing: performing origin discrimination by using an unknown sample in the model, and ultimately determining attributes of the origin.2. The method for tracing wine origin based on multielements and stable isotopes according to claim 1 , characterized in that the carbon stable isotope analysis comprises steps of: switching on a host machine of a stable isotope ratio mass spectrometer and adjusting a reference gas balance claim 1 , such that a variation of the carbon stable isotope ratios of COreference gases is less than 0.06‰.3. The method for tracing wine origin based on multielements and stable isotopes according to claim 1 , characterized ...

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Номер записи: 37
08-03-2018 дата публикации

CANCER CELL ENRICHMENT SYSTEM

Номер: US20180066223A1
Автор: CASSEREAU Luke, LIM James
Принадлежит:

Embodiments of the invention relate to a cell culture incubator having a gas flow regulation system that exerts control over atmospheric parameters within the incubator. Particular embodiments include an enclosed environmental chamber and a control unit operably linked thereto, the control unit having an oxygen module and a pressure module. Control unit embodiments, by way of these modules, are configured to regulate both oxygen partial pressure and total gas pressure within the enclosed environmental chamber. Embodiments of the control unit are adapted (a) to provide instructions to the oxygen module to regulate an oxygen level to an instructed hypoxic oxygen level and (b) to provide instructions to the pressure module to regulate total gas pressure to an instructed positive pressure level. The regulation of oxygen to the instructed hypoxic level prevails despite an oxygen partial pressure-increasing effect of the positive pressure condition associated with the instructed positive pressure level. 126-. (canceled)27. A cell culture incubator comprising:an enclosed environmental chamber; anda control unit operably linked to the enclosed environmental chamber, wherein the control unit comprises an oxygen module and a pressure module, a) provide instructions to the oxygen module to regulate the oxygen level to an instructed hypoxic oxygen level; and', 'b) provide instructions to the pressure module to regulate the total gas pressure to an instructed positive pressure level,, 'wherein the control unit is configured to regulate each of an oxygen level and a total gas pressure within the enclosed environmental chamber, and wherein the control unit is adapted towherein the regulation of the oxygen level to the instructed hypoxic level prevails despite an oxygen partial pressure increasing effect of a positive pressure condition, per the instructed positive pressure level.28. The cell culture incubator of claim 27 , further comprising:an oxygen sensor configured to measure ...

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Номер записи: 38
12-03-2015 дата публикации

SYSTEM AND METHOD FOR DETECTION OF A CONTAMINATED BEVERAGE

Номер: US20150071831A1
Автор: Abramson Michael T., MacDonald John C.
Принадлежит:

A system and method for a beverage container configured to test for a contaminated beverage in the beverage container comprises a base of the beverage container, an upper portion of the beverage container, and a sidewall with an inner surface and an outer surface that extends from the base to the upper portion, and a testing material. A portion of the beverage container is the testing material configured to visibly react when the portion of the beverage container is contacted with the contaminated beverage. 1. An apparatus configured to test a beverage , comprising:a beverage container configured to hold a beverage, wherein the beverage container comprises a material; andan indicator chemically bonded to at least a portion of the material of the beverage container, wherein the indicator visibly reacts when the indicator is contacted with alcohol to indicate the alcohol is present in the beverage, wherein at least the portion of the material of the beverage container comprises the indicator, and wherein the visible reaction is shown on at least the portion of the material of the beverage container via the indicator chemically bonded to at least the portion of the material of the beverage container.2. The apparatus of wherein at least the portion of the material of the beverage container comprises a film claim 1 , and wherein the film comprises the chemically bonded indicator.3. The apparatus of wherein the visible reaction is shown on at least the portion of the material of the beverage container via the indicator chemically bonded to at least the portion of the material of the beverage container when a concentration threshold of the alcohol is present in the beverage.4. The apparatus of wherein the indicator is at least one of pre-attached and post-attached to at least the portion of the material of the beverage container.5. The apparatus of further comprising a second indicator chemically bonded to at least a second portion of the material of the beverage container ...

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Номер записи: 39
11-03-2021 дата публикации

Cell Culture Incubator System

Номер: US20210071129A1
Автор: Cremonese Joseph G.
Принадлежит:

Disclosed is a cell culture incubator system having a sensor strip configured to be placed within a reaction vessel (e.g., incubator vessel) so as to position the sensor within media covering the cells. A reader is placed outside but adjacent the vessel to read the sensor so as to detect changes in dissolved Oand pH. The system is used to determine if the incubator environment has too much COand is therefore trending towards hypoxia and/or acidity, or has too much dissolved Oand is therefore trending toward oxygen toxicity via the cell nutrient media. In some embodiments, the system includes a rocker unit configured to rock the reaction vessel to enhance cell growth. In some embodiments, the system includes a gas circulation system to adjust the COand dissolved Olevels in the reaction vessel. 1. A sensor strip , comprising:a strip comprising a first end and a second end;the first end having a fluorescence-based sensor disposed or attached thereto, the first end being further configured to be inserted into a reaction vessel; andthe second end having a stopper configured to engage with a mouth of the reaction vessel so as to secure the sensor strip to the reaction vessel and to position the fluorescence-based sensor at a bottom of the reaction vessel or suspend the fluorescence-based sensor at a distance above the bottom the reaction vessel.2. The sensor strip of claim 1 , comprising a plurality of fluorescence-based sensors disposed or attached to the first end.3. The sensor strip of claim 1 , comprising:a first fluorescence-based sensor disposed or attached to the first end, the first fluorescence-based sensor configured to detect a pH level; anda second fluorescence-based sensor disposed or attached to the first end, the second fluorescence-based sensor configured to detect a dissolved oxygen level.4. The sensor strip of claim 1 , wherein the suspended position of the fluorescence-based sensor at a distance above the bottom the reaction vessel includes positioning ...

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Номер записи: 40
05-03-2020 дата публикации

Liquid transfer system

Номер: US20200070144A1
Автор: Alan Blanchard
Принадлежит: Thrive Bioscience Inc

Aspects of the invention relate to liquid transfer systems including a pipette tip having a body defining an inner volume and having first and second ends, the first end arranged to transfer a volume of liquid into and out of the pipette tip and the second end arranged for attachment to a pipette, and a bladder sealingly attached to the body. In some embodiments, the bladder is disposable in the inner volume. The bladder may be defined by a membranous sac and/or a planar membrane. The bladder may be moveable in response to an applied pressure to transfer liquid. The bladder also may be stretchable to accommodate a volume of fluid. In some embodiments, the pipette tip is used in a cell culture incubator, with a manipulator including a pipette that is in fluid communication with the pipette tip.

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Номер записи: 41
16-03-2017 дата публикации

TESTING METHODS AND APPARATUS

Номер: US20170073721A1
Автор: Brooking Annie, Price Derek, RICKETT Toby William
Принадлежит:

We describe a method of selectively testing a liquid sample for the presence of a specific living biological entity, the method comprising: preparing a plurality of culture vessels each for selective detection of the presence of a different living biological entity, wherein preparing a said culture vessel comprises: providing a culture vessel comprising a sealable chamber for receiving and culturing a liquid sample such that presence of said biological entity is detectable by detecting a change in pressure in a head space of said chamber; providing a selective growth medium in said culture vessel, wherein said selective growth medium is in a dry form and selectively facilitates growth of a specific living biological entity, and providing the culture vessel with an identifier to identity said specific biological entity; providing a test system comprising a device to receive said culture vessel and to detect said change in pressure in said head space of said chamber, the device further comprising a temperature control system, and a data processor to monitor said detected change in pressure, to control said temperature control system to regulate a temperature of a liquid sample in said culture vessel, and to signal detection of the presence of a biological entity in said culture vessel from said detected change in pressure; storing a plurality of entity—selective test protocols in a memory of said test system, wherein a said test protocol defines one or both of a temperature of incubation of said liquid sample in said culture vessel and a threshold change in pressure in said head space of said chamber for detection of the target entity, wherein said test protocols are respectively identified according to one of a plurality of specific biological entities for which they selectively facilitate growth; selecting, from said plurality of culture vessels, a culture vessel to selectively detect a target said specific biological entity; providing a test liquid sample to said ...

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Номер записи: 42
05-03-2020 дата публикации

BIOREACTION CONTAINER

Номер: US20200071654A1
Автор: HEO Sang Oh, KIM Dong Hoon, KIM Doo Hyun, OH Duk Jae
Принадлежит:

A bioreaction container including a culture chamber configured to contain a culture solution and a life form in an inner space, the culture chamber having an open upper end; a chamber cover portion coupled to the upper end of the culture chamber, the chamber cover portion having a protruding tube provided on one side thereof so as to communicate with the inner space; a filter cap coupled to the protruding tube in an attachable/detachable manner so as to open/close the protruding tube; a gas introduction portion configured to penetrate the chamber cover portion and to communicate with the inner space so as to supply a predetermined gas into the inner space; and an acidity/basicity adjustment portion installed on the chamber cover portion while containing an adjustment solution that adjusts pH of the culture solution such that the adjustment solution is discharged into the inner space by pneumatic pressure. 110.-. (canceled)11. A bioreaction container comprising:a culture chamber configured to contain a culture solution and a life form in an inner space, the culture chamber having an open upper end;a chamber cover portion coupled to the upper end of the culture chamber, the chamber cover portion having a protruding tube provided on one side thereof so as to communicate with the inner space;a filter cap coupled to the protruding tube in an attachable/detachable manner so as to open/close the protruding tube;a gas introduction portion configured to penetrate the chamber cover portion and to communicate with the inner space so as to supply a predetermined gas into the inner space; andan acidity/basicity adjustment portion installed on the chamber cover portion while containing an adjustment solution that adjusts pH of the culture solution such that the adjustment solution is discharged into the inner space by pneumatic pressure, whereinacidity/basicity adjustment portion comprises:a storage tank having a containing space in which the adjustment solution is stored, the ...

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Номер записи: 43
05-03-2020 дата публикации

SYSTEM AND METHOD FOR MONITORING AND CONTROLLING CONDITIONS WITHIN A VESSEL

Номер: US20200071655A1
Автор: Palmaz Christian Gaston
Принадлежит:

A system and method for regression modeling an interior volume of a containment vessel and interpolating data from multi-point sensor arrays within the containment vessel to detect conditions across the interior volume of the containment vessel. 1. A system for monitoring and controlling conditions within a containment vessel , comprising:a. a plurality of sensor arrays, each of the plurality of sensor arrays having a length configured to allow each sensor array to extend between an inner bottom surface and an inner top surface of the containment vessel; each of the plurality of sensor arrays having a plurality of sensors spaced along the length of the sensor array, each of the plurality of sensors configured to sense a condition to be monitored or controlled within the containment vessel;b. at least one of the plurality of sensor arrays being positioned at or proximate to a center position of the containment vessel and at least one of the plurality of sensor arrays being positioned at or proximate to an inner peripheral wall of the containment vessel such that each of the plurality of sensors are in vertical alignment between the plurality of sensor arrays;c. a computer in communication with each of the plurality of sensors which receives signals from each of the plurality of sensors corresponding to the condition sensed by each of the plurality of sensors; andd. a processor configured to process the signals from each of the plurality of sensors and interpolate the signals across a substantial area within the containment vessel.2. The system according to claim 1 , further comprising a first housing passing into the containment vessel and positioned along a central axis of the containment vessel and a second housing passing into the containment vessel and positioned proximate to a side wall of the containment vessel claim 1 , at least one of the plurality of sensor arrays being disposed in the first housing and second housing.3. The system according to claim 2 , ...

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Номер записи: 44
12-03-2020 дата публикации

AUTOMATED CELL GROWTH AND/OR CONCENTRATION MODULES AS STAND-ALONE DEVICES OR FOR USE IN MULTI-MODULE CELL PROCESSING INSTRUMENTATION

Номер: US20200080045A1
Автор: BELGRADER Phillip, Bernate Jorge, Chabansky Bruce, Masquelier Don
Принадлежит:

The present disclosure provides a cell growth, buffer exchange, and/or cell concentration/filtration device that may be used as a stand-alone device or as a module configured to be used in an automated multi-module cell processing environment. 1. A method for concentrating a cell sample , comprising the steps of: [ a retentate member comprising an upper surface and a lower surface with a retentate channel structure disposed on the lower surface of the retentate member and first and second retentate ports wherein the first retentate port is disposed at a first end of the retentate channel structure and the second retentate port is disposed at a second end of the retentate channel structure, and wherein the first and second retentate ports traverse the first member from the lower surface to the upper surface;', 'a permeate member comprising an upper surface and a lower surface with a permeate channel structure disposed on the upper surface of the permeate member and at least one permeate port, wherein the at least one permeate port is disposed at a first end of the permeate channel structure, wherein the at least one permeate port traverses the permeate member from the lower surface to the upper surface, and wherein the retentate and permeate channel structures mate to form a single flow channel; and', 'a membrane disposed between the retentate and permeate members thereby bifurcating the single flow channel into upper and lower portions;, 'a tangential flow assembly comprising, 'a reservoir assembly comprising a first retentate reservoir fluidically coupled to the first retentate port, a second retentate reservoir fluidically coupled to the second retentate port and a reservoir top disposed over the first and second retentate reservoirs;', 'a pneumatic assembly configured to apply pressure to move liquid through the single flow channel via negative and positive pressure applied to the first and second retentate reservoirs, to monitor pressure in the retentate ...

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Номер записи: 45
25-03-2021 дата публикации

CELL CULTURE SYSTEM AND CELL CULTURE METHOD

Номер: US20210087521A1
Автор: IKEDA Ryosuke, Iso Yoshiyuki, KAMEKURA Koichi, MIZUNUMA Takato, TOMIMATSU Hirofumi
Принадлежит: IHI CORPORATION

The cell culture system has a culture tank containing a liquid medium that cultures cells, and a cell separator including a hydrodynamic separation device and a liquid feeding unit. The hydrodynamic separation device has a curved flow channel having a rectangular cross-section, and separates relatively large cells from the cells contained in the liquid medium using a vortex flow generated by flow through the curved flow channel. The liquid feeding unit flows the liquid medium through the hydrodynamic separation device in a pressure environment controlled to suppress decrease in cell viability caused by pressure fluctuation in the liquid medium during flowing through the hydrodynamic separation device. 1. A cell culture system , comprising a culture tank that contains a liquid medium that cultures cells , and a cell separator , the cell separator comprising:a hydrodynamic separation device having a curved flow channel having a rectangular cross-section, and separating relatively large cells from the cells contained in the liquid medium using a vortex flow generated by flow through the curved flow channel; anda liquid feeding unit which flows the liquid medium through the hydrodynamic separation device in a pressure environment controlled so as to suppress decrease in cell viability caused by pressure fluctuation in the liquid medium during flowing through the hydrodynamic separation device.2. The cell culture system according to claim 1 , wherein the hydrodynamic separation device has a single inlet for taking in the liquid medium and at least two outlets for discharging the separated liquid medium claim 1 , wherein a liquid medium containing the relatively large cells concentrated is discharged from one of the at least two outlets claim 1 , and the rest of the liquid medium containing relatively small cells is discharged from the other of the at least two outlets.3. The cell culture system according to claim 1 , wherein the liquid feeding unit has an urging device ...

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Номер записи: 46
29-03-2018 дата публикации

CELL CULTURE INCUBATORS WITH INTEGRATED CELL MANIPULATION SYSTEMS

Номер: US20180087021A1
Автор: BLANCHARD ALAN
Принадлежит: Thrive Bioscience, Inc.

Aspects of the invention relate to automated cell culture incubators. In some embodiments, automated cell culture incubators comprise an integrated manipulation having one or more cell scrapers. 1. A cell culture incubator comprising:an incubator cabinet comprising an internal chamber for incubation of cells in one or more cell culture vessels;a door opening from an external environment to said internal chamber;an imager configured for imaging said cells within said internal chamber;a manipulator for manipulating said cells in said one or more cell culture vessels within said internal chamber; and,a cell culture vessel transfer device for moving said one or more cell culture vessels between locations within said internal chamber.2. The incubator of claim 1 , wherein said imager is a holographic microscope.3. The incubator of claim 1 , wherein said imager is a bright-field microscope.4. The incubator of claim 1 , wherein said imager is a fluorescence microscope.5. The incubator of any one of - claim 1 , wherein said one or more cell culture vessels comprise fiducial marks for facilitating alignment of said one or more cell culture vessels to said imager and said manipulator.6. The incubator of any one of - claim 1 , further comprising an imaging location claim 1 , wherein the imaging location comprises fiducial marks.7. The incubator of any one of - claim 1 , wherein said manipulator for manipulating said cells is a cell picker.8. The incubator of any one of - claim 1 , further comprising a controller to control said manipulator.9. The incubator of claim 8 , wherein said controller is located exterior to said incubator cabinet.10. The incubator of claim 9 , wherein said controller comprises a computer.11. A cell culture incubator comprising:an incubator cabinet comprising an internal chamber for incubation of cells in one or more cell culture vessels;a door opening to said internal chamber;a holographic imager and a first imaging location, said holographic imager ...

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Номер записи: 47
05-05-2022 дата публикации

PHOTOBIOREACTOR FOR BLUE-GREEN ALGAE CULTIVATION

Номер: US20220135932A1
Автор: GALBIATI Cristiano
Принадлежит:

The present invention concerns the field of high-efficiency, quality-controlled production of blue-green algae for direct human consumption, for extraction of proteins, vitamins, and amino acids, and for production of organic materials loaded with the special isotope 13C.

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Номер записи: 48
19-03-2020 дата публикации

Large scale cell growing devices and systems

Номер: US20200087604A1
Автор: Justin Zdeb, Stephen John Pearson
Принадлежит: Individual

A cell-growing system may include a cell-growing container, a pump for controlling the pressure of the chamber of the container, a filter for maintaining the chamber sterilized, a flow control regulator for controlling the substance supplied to the cell-growing container. The cell-growing container may include an internal structure that takes the form of a reticulated structure to increase the surface area for growing cells. The cell-growing system may be used to perform cycles of cell expansion and harvest. The cell-growing system may be maintained as a closed and sterilized system through the cycles. In harvesting, a first subset of the cells can be disassociated from the cell culture while a second subset remains. The disassociated cells may be discharged to a collection container while maintaining the system closed to an external environment. The remaining cells may be used as seeds to proliferate more cells in the next cycle.

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Номер записи: 49
16-04-2015 дата публикации

SYSTEM AND METHOD FOR DETECTION OF A CONTAMINATED BEVERAGE

Номер: US20150104861A1
Автор: Abramson Michael T., Landrau Nelson, MacDonald John C., Nivorozhkin Alex, Walker Brendan
Принадлежит:

An apparatus may be configured to test a beverage, the apparatus may include but is not limited to, a beverage container that may be configured to hold a beverage, wherein the beverage container may include a material. An indicator may be chemically bonded to at least a portion of the material of the beverage container, wherein at least the portion of the material of the beverage container may include the indicator, wherein the indicator may be associated with at least part of a brand profile to identify a beverage brand's beverage, wherein the indicator may visibly react when the indicator is contacted with the beverage to show whether the beverage may be identified as the beverage brand's beverage, and wherein the visible reaction may be shown on at least the portion of the material of the beverage container via the indicator chemically bonded to at least the portion of the material of the beverage container. 1. An apparatus configured to test a beverage for brand verification , comprising:a beverage container configured to hold a beverage, wherein the beverage container includes a material; andan indicator chemically bonded to at least a portion of the material of the beverage container, wherein at least the portion of the material of the beverage container includes the indicator, wherein the indicator is associated with a brand profile that identifies a beverage brand's beverage, wherein the indicator visibly reacts when the indicator is contacted with the beverage to show whether the beverage is identified as the beverage brand's beverage, and wherein the visible reaction is shown on at least the portion of the material of the beverage container via the indicator chemically bonded to at least the portion of the material of the beverage container.2. The apparatus of wherein the indicator visibly reacts when the indicator is at least one of contacted with a substance in the beverage included in the brand profile and contacted with the beverage lacking the ...

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Номер записи: 50
08-04-2021 дата публикации

System for Deep Sediment Flow Culture Simulating In-situ Water Pressure

Номер: US20210101141A1
Автор: Chen Qiuwen, CHEN YUCHEN, KUI Lanlin, LIU Dongsheng, MA Honghai, ZHANG Jianyun, ZHANG Qi, ZHU Haoyu
Принадлежит:

It discloses a system for deep sediment flow culture simulating in-situ water pressure, comprising a flow culture apparatus, an inflow pressurizer and an outflow depressurizer, wherein the inflow pressurizer comprises a pressure tank, an air inlet pipe, a pressure regulating valve, a pressure-resistant container and a first support, wherein the pressure tank is connected with an air inlet of the pressure-resistant container through the air inlet pipe, the pressure regulating valve is arranged on the air inlet pipe, and the pressure-resistant container containing in-situ overlying water added with isotopes is placed on the first support, a water outlet of the pressure-resistant container being connected with a water inlet pipe of the flow culture apparatus; the outflow depressurizer comprises a porous medium pipe, a second support, a depressurized water outlet pipe and a water catcher. 1. A system for deep sediment flow culture simulating in-situ water pressure , comprising a flow culture apparatus , and an inflow pressurizer and an outflow depressurizer each connected with the flow culture apparatus , wherein the inflow pressurizer comprises a pressure tank , an air inlet pipe , a pressure regulating valve , a pressure-resistant container and a first support , wherein the pressure tank is connected with an air inlet of the pressure-resistant container through the air inlet pipe , the pressure regulating valve is arranged on the air inlet pipe , and the pressure-resistant container containing in-situ overlying water added with isotopes is placed on the first support , a water outlet of the pressure-resistant container being connected with a water inlet pipe of the flow culture apparatus; the outflow depressurizer comprises a porous medium pipe , a second support , a depressurized water outlet pipe and a water catcher , wherein the porous medium pipe is filled with a porous medium material and placed on the second support , wherein an inlet of the porous medium pipe ...

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Номер записи: 51
12-04-2018 дата публикации

CANCER CELL ENRICHMENT SYSTEM

Номер: US20180100134A1
Автор: CASSEREAU Luke, LIM James
Принадлежит:

The present invention describes a method for the propagation of a subpopulation of cells. The subpopulation of cells can be grown in specific culture conditions to promote growth and enrichment of the subpopulation of cells. The method can be used to determine biomarkers, and guide treatment of cancer patients. 126-. (canceled)27. A cell culture incubator , wherein the cell culture incubator comprises:a) an enclosed environmental chamber; and (i) an oxygen level module which receives an input to adjust an oxygen level in the enclosed environmental chamber to generate an instructed oxygen level within the environmental chamber wherein the instructed oxygen level is an instructed hypoxic oxygen level;', '(ii) a pressure module which receives an input to adjust pressure in the enclosed environmental chamber to generate an instructed pressure level within the environmental chamber wherein the instructed pressure level is an instructed positive pressure level;', '(iii) a temperature module which receives an input to adjust temperature in the enclosed environmental chamber to generate an instructed temperature within the environmental chamber; and', '(iv) a humidity module which receives an input to adjust humidity in the enclosed environmental chamber to generate an instructed humidity level within the environmental chamber., 'b) a control unit operably linked to the enclosed environmental chamber, wherein the control unit comprises a computer program product, wherein the computer program product comprises a computer-readable medium, wherein the computer-readable medium comprises a computer-executable code encoded, wherein the computer-executable code is adapted to instruct28. The cell culture incubator of claim 27 , wherein:a) the enclosed environmental chamber reaches the instructed hypoxic oxygen level within at least one hour of the oxygen level module receiving the input to adjust the oxygen level within the enclosed environmental chamber;b) the enclosed ...

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Номер записи: 52
21-04-2016 дата публикации

System for switching over the exhaust air of a bioreactor

Номер: US20160108354A1
Автор: Bernward Husemann, Ralf Hartmann, Wolfgang Kahlert
Принадлежит: SARTORIUS STEDIM BIOTECH GMBH

A system ( 1 ) is provided for switching over exhaust air of a bioreactor ( 2 ) that has a disposable container ( 3 ). The system ( 1 ) has at least two exhaust air ducts ( 4, 5 ) connecting the disposable container ( 3 ) to exhaust air filters ( 6, 7, 18, 18′ ). A valve ( 8 ) is disposed in at least one of the at least two exhaust air ducts ( 4, 5 ) and enables the associated exhaust air filter ( 6, 7, 18, 18 ′) to be freed.

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Номер записи: 53
09-06-2022 дата публикации

MICROFLUIDIC SYSTEM FOR INTRACELLULAR DELIVERY OF MATERIALS AND METHOD THEREFOR

Номер: US20220177819A1
Автор: CHUNG Aram, HUR Jeong-Soo, KANG Geoum-Young
Принадлежит: MxT BIOTECH

There is provided a microfluidic system delivering external materials into a cell by cell mechanoporation using inertia, the microfluidic system including a fluidic channel structure through which a solution containing a cell and external materials flows continuously, in which the fluidic channel structure includes a junction between one or more channels, a localized vortex is generated near an interface of the junction, the cell is deformed by the vortex, and transient discontinuities are generated in a cell membrane by the vortex and the external materials are introduced into the cell by solution exchange between the cell and fluid around the cell. 1. A microfluidic system delivering external materials into a cell by cell mechanoporation using inertia , the microfluidic system comprising:a fluidic channel structure through which a solution containing a cell and external materials flows continuously,wherein the fluidic channel structure includes a junction between one or more channels,a localized vortex is generated near an interface of the junction,the cell is deformed by the vortex, andtransient discontinuities are generated in a cell membrane by the vortex and the external materials are introduced into the cell by solution exchange between the cell and fluid around the cell.2. The microfluidic system of claim 1 , wherein the fluidic channel structure including the junction between one or more channels includes a junction including a T claim 1 , Y claim 1 , cross shape claim 1 , or a combination thereof.3. The microfluidic system of claim 2 , wherein the fluidic channel structure includes a cavity near a fluid stagnation point when the fluidic channel structure is a channel of the T or Y shape.4. The microfluidic system of claim 3 , wherein the cavity has a shape of a circle claim 3 , an ellipse claim 3 , an elongate slit claim 3 , a square claim 3 , a rectangle claim 3 , a trapezoid claim 3 , a polygon claim 3 , and a combination thereof claim 3 , and a ...

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Номер записи: 54
17-07-2014 дата публикации

METHOD OF MEASUREMENT OF GLUTEN CONTENT IN A SAMPLE OF FLOUR

Номер: US20140196533A1
Автор: SCHULTZ Irma
Принадлежит:

The present invention relates to a method for measuring the gluten content in a flour sample. The method includes preparing the flour, sifting it, and adding water, then pre-hydrating with water in a pre-hydrating mixer for a few seconds, until a sphere cohesive material (SCM) is obtained. Then, the sphere SCM is submerged in a container with salt water for a few minutes, to allow the structuring of the gluten. Then, the sphere is washed in a washing machine to eliminate the water soluble substances, which are not gluten. Then, the gluten structured with excess water is centrifuged in a “centrifuge”, then removed from the centrifuge and weighed. The value that is read is the measurement of wet gluten. To obtain the value of the dry gluten, dry in a desiccation stove or a Teflon® dryer. 1. A method of measurement of a gluten content in a flour sample , the method comprising the steps of:1) weighing between 5 to 30 grams of a milled flour sample, passing the weighted flour sample to a hydrating mixer, adding between 3 and 8 ml of a saline solution;2) mixing during 14 to 30 seconds until forming a sphere of cohesive material SCM.;3) submerging the sphere of step 2 into a container with the saline solution for 20 to 60 minutes or wrapping the sphere SCM in a filter paper wetted with the saline solution for 40 and 80 minutes;4) placing the sphere obtained in step 3 into a washer machine and washing the sphere until a wash water loses turbidity;5) placing the sphere obtained in step 4 in a centrifugal and centrifuge for 0.5 to 3 minutes at 6000 RPM; and(6) weighing the sphere of step 5 to obtain a wet gluten and calculating a percentage of wet gluten, drying the wet gluten for 2 to 15 minutes in a Teflon® press at 150° C. or at 105° C. in a laboratory drying oven, weighing the dry gluten and calculating a percentage of dry gluten.2. A method of measurement of a gluten content in a flour sample , the method comprising the steps of:1) weighing 10 grams of a milled flour ...

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Номер записи: 55
27-04-2017 дата публикации

NONINVASIVE REFRACTOMETER

Номер: US20170115214A1
Автор: HARJUNMAA Hannu, Harjunmaa Sinikka
Принадлежит:

The present invention pertains to the measurement of the refractive index of a medium, such as a fluid, through the wall of its container. The essential characteristic of the invention is that, by using at least two separate light paths that are of unequal length and that reflect from the wall/medium interface, it is possible to perform the measurement of the refractive index of the medium so that the result is insensitive to the color and thickness of the wall. 1. A method for measuring the refractive index of a medium situated behind a transparent wall , wherein at least two light beams are arranged to illuminate the wall/medium interface through the wall at different angles and wherein the Fresnel reflections of the beams from the wall/medium interface are detected by at least one detector.2. A method according to claim 1 , wherein there are at least two light sources and one detector.3. A method according to claim 1 , wherein there is one light source and at least two detectors.4. A method according to claim 1 , wherein there is at least two light sources and at least two detectors.5. A method according to claim 1 , wherein the Fresnel reflection from the wall/medium interface is compared to the Fresnel reflection from a reference interface detected by the same detector.6. A method according to claim 1 , where the medium is a beverage and the wall is a bottle wall.7. A method according to claim 1 , where the beverage is wine or liqueur.8. An Apparatus according to claim 1 , comprising light sources and detectors claim 1 , the total number of them combined being at least three claim 1 , an optical setup that defines at least two lightpaths of different lengths from said light sources twice through the wall of a container to said detectors claim 1 , a control unit and a display.9. An apparatus according to claim 8 , where the reference interface is separate from the device.10. An apparatus according to claim 8 , where the reference interface is contained within ...

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Номер записи: 56
05-05-2016 дата публикации

Non-Invasive Wine Taint Detector

Номер: US20160123876A1
Автор: Muldoon Cecilia
Принадлежит:

A system includes a computing device including a memory configured to store instructions. The computing device also includes a processor to execute the instructions to perform operations including initiating transmission of incident light from one or more light sources to a sealed bottle containing liquid. The operations also include receiving scattered light from the liquid contained in the sealed bottle. The operations also include processing one or more signals representative of the scattered light to detect interactions of the incident light with a particular molecule. 1. A computing device implemented method comprising:initiating transmission of incident light from one or more light sources to a sealed bottle containing liquid;receiving scattered light from the liquid contained in the sealed bottle; andprocessing one or more signals representative of the scattered light to detect interactions of the incident light with a particular molecule.2. The computing device implemented method of claim 1 , further comprising:filtering the scattered light prior to processing one or more signals representative of the scattered light.3. The computing device implemented method of claim 1 , wherein processing one or more signals representative of the scattered light comprises filtering signals.4. The computing device implemented method of claim 3 , wherein filtering the scattered light comprises passing frequencies of the received scattered light that are within a particular frequency range.5. The computing device implemented method of claim 1 , further comprising:spectrally separating the scattered light prior to processing one or more signals representative of the scattered light.6. The computing device implemented method of claim 1 , wherein the liquid is wine.7. The computing device implemented method of claim 1 , wherein the particular molecule is trichloroanisole or methanethiol.8. The computing device implemented method of claim 7 , wherein processing comprises ...

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Номер записи: 57
04-05-2017 дата публикации

SYSTEMS AND METHODS FOR IMPROVED PERFORMANCE OF FLUIDIC AND MICROFLUIDIC SYSTEMS

Номер: US20170121658A1
Автор: Fernandez-Alcon Jose, Geisler Hubert, Hinojosa Christopher David, Ingber Donald E., Levner Daniel, Sliz Josiah, Thompson Guy
Принадлежит:

Systems and methods for improved flow properties in fluidic and microfluidic systems are disclosed. The system includes a microfluidic device having a first microchannel, a fluid reservoir having a working fluid and a pressurized gas, a pump in communication with the fluid reservoir to maintain a desired pressure of the pressurized gas, and a fluid-resistance element located within a fluid path between the fluid reservoir and the first microchannel. The fluid-resistance element includes a first fluidic resistance that is substantially larger than a second fluidic resistance associated with the first microchannel. 1. A system for monitoring a biological function associated with cells , comprising:a microfluidic device having a first microchannel, a second microchannel, and a membrane located at an interface region between the first microchannel and the second microchannel, the membrane including a first side facing toward the first microchannel and a second side facing toward the second microchannel, the membrane having the cells adhered thereto;a fluid line for delivering a working fluid to or from the first microchannel from or to, respectively, a fluid reservoir; anda fluid-resistance element coupled to the fluid line, the fluid-resistance element comprising a substrate having an elongated fluid path, thereby having a first fluidic resistance that is substantially larger than a second fluidic resistance associated with the first microchannel.2. The system of claim 1 , wherein the fluid reservoir includes the working fluid and a pressurized gas claim 1 , the pressurized gas forcing the flow of the working fluid through the fluid line and fluid-resistance element.3. The system of claim 1 , wherein the substrate of said fluid-resistance element comprises a chip having an elongated fluid path claim 1 , the first fluidic resistance being created by the elongated fluid path.4. The system of claim 1 , wherein the substrate of said fluid-resistance element comprises an ...

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Номер записи: 58
04-05-2017 дата публикации

SYSTEMS AND METHODS FOR IMPROVED PERFORMANCE OF FLUIDIC AND MICROFLUIDIC SYSTEMS

Номер: US20170121659A1
Автор: Fernandez-Alcon Jose, Geisler Hubert, Hinojosa Christopher David, Ingber Donald E., Levner Daniel, Sliz Josiah, Thompson Guy
Принадлежит:

Systems and methods for improved flow properties in fluidic and microfluidic systems are disclosed. The system includes a microfluidic device having a first microchannel, a fluid reservoir having a working fluid and a pressurized gas, a pump in communication with the fluid reservoir to maintain a desired pressure of the pressurized gas, and a fluid-resistance element located within a fluid path between the fluid reservoir and the first microchannel. The fluid-resistance element includes a first fluidic resistance that is substantially larger than a second fluidic resistance associated with the first microchannel. 1. A microfluidic system , comprising:a cartridge removably interfaced with a microfluidic device having a first microchannel;a fluid reservoir;a fluid line for delivering a working fluid to or from the first microchannel from the fluid reservoir; anda fluid-resistance element within the fluid line, the fluid-resistance element having a first fluidic resistance that is substantially larger than a second fluidic resistance associated with the first microchannel.2. The microfluidic system of claim 1 , wherein said fluid-resistance element is attached to said cartridge.3. The microfluidic system of claim 1 , wherein said fluid-resistance element is removably attached to said cartridge.4. The microfluidic system of claim 1 , wherein said fluid-resistance element comprises a substrate having an elongated fluid path.5. The microfluidic system of claim 4 , wherein said substrate of said fluid-resistance element comprises an elongated tube having an elongated fluid path claim 4 , the first fluidic resistance being created by the elongated fluid path.6. The microfluidic system of claim 5 , wherein the tube undergoes multiple windings so as to create said elongated path.7. A microfluidic system claim 5 , comprising:a cartridge configured to be removably interfaced with a microfluidic device having a first microchannel;a fluid reservoir having a working fluid and a ...

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Номер записи: 59
04-05-2017 дата публикации

SYSTEMS AND METHODS FOR IMPROVED PERFORMANCE OF FLUIDIC AND MICROFLUIDIC SYSTEMS

Номер: US20170121663A1
Автор: Fernandez-Alcon Jose, Geisler Hubert, Hinojosa Christopher David, Ingber Donald E., Levner Daniel, Sliz Josiah, Thompson Guy
Принадлежит:

Systems and methods for improved flow properties in fluidic and microfluidic systems are disclosed. The system includes a microfluidic device having a first microchannel, a fluid reservoir having a working fluid and a pressurized gas, a pump in communication with the fluid reservoir to maintain a desired pressure of the pressurized gas, and a fluid-resistance element located within a fluid path between the fluid reservoir and the first microchannel. The fluid-resistance element includes a first fluidic resistance that is substantially larger than a second fluidic resistance associated with the first microchannel. 1. A method of pressure driven flow , comprising: a microfluidic device having a microchannel;', 'a fluid reservoir comprising a working fluid;', 'a fluid line for delivering said working fluid from said fluid reservoir to or from the microchannel; and', 'a fluid-resistance element coupled to the fluid line, the fluid-resistance element comprising a substrate having an elongated fluid path, thereby having a first fluidic resistance that is substantially larger than a second fluidic resistance associated with the microchannel; and, 'a) providingb) forcing the working fluid through the fluid line and the fluid-resistance element with pressurized gas such that said working fluid flows through said microchannel at a flow rate.2. The method of claim 1 , wherein the fluid reservoir includes a pressurized gas.3. The method of claim 1 , wherein the substrate of said fluid-resistance element comprises a chip having an elongated fluid path claim 1 , the first fluidic resistance being created by the elongated fluid path.4. The method of claim 3 , wherein the substrate of said fluid-resistance element comprises an elongated tube having an elongated fluid path claim 3 , the first fluidic resistance being created by the elongated fluid path.5. The method of claim 4 , wherein the tube undergoes multiple windings so as to create said elongated path.6. The method of claim 1 ...

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Номер записи: 60
14-05-2015 дата публикации

DEVICE AND METHOD FOR CONTINUOUS CELL CULTURE AND OTHER REACTIONS

Номер: US20150132845A1
Автор: Lee Kevin Shao-Kwan, Ram Rajeev Jagga
Принадлежит: Massachusetts Institute of Technology

Devices, systems, and methods for continuous cell culture and other reactions are generally described. In some embodiments, chambers (e.g., cell growth chambers) including at least a portion of a wall formed of a flexible member are provided. A retaining structure can be incorporated outside and proximate to the chamber such that when liquid is added to the chamber, the flexible member is consistently and predictably deformed, and a consistent volume of liquid is added. The flexible member can be formed of, in some embodiments, a gas-permeable medium. In some embodiments, reaction chambers can be arranged in a fluidic loop, and a bypass channel can be used to introduce and/or extract fluid from the loop without affecting loop operation. 154-. (canceled)55. An article for transporting liquid , comprising:a first chamber comprising a first wall and a second wall, with at least a portion of the first wall of the first chamber defined by a first flexible member portion;a first inlet and a first outlet fluidically connected to the first chamber;a second chamber comprising a first wall and a second wall, with at least a portion of the first wall of the second chamber defined by the first flexible member portion;a second inlet fluidically connected to the second chamber;a third chamber comprising a first wall and a second wall, with at least a portion of the first wall of the third chamber defined by a second flexible member portion;a third inlet and a third outlet fluidically connected to the third chamber;a fourth chamber comprising a first wall and a second wall, with at least a portion of the first wall of the fourth chamber defined by the second flexible member portion;a fourth inlet fluidically connected to the fourth chamber;a first fluidic pathway connected to the first outlet and the third inlet;a first valve fluidically connected to the first chamber and to the first inlet;a second valve fluidically connected to the third chamber and to the third outlet;a bypass ...

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Номер записи: 61
11-05-2017 дата публикации

MICROFLUIDIC PRESSURE REGULATOR FOR ROBUST HYDROGEL LOADING WITHOUT BURSTING

Номер: US20170130187A1
Автор: George Steven C., Hughes Christopher C.W., Lee Abraham P., Phan Duc, Wang Xiaolin
Принадлежит:

A pressure regulator module for a chip-based microfluidic platform is provided. The module includes a microfluidic channel for passing flowable material from the inlet region through the outlet region and into a downstream compartment; one or more microvalves fluidly connected to the microfluidic channel and upstream of the outlet region; and one or more reservoirs fluidly connected to the microvalves, for receiving flowable material diverted by the microvalves, where a flow of flowable material passing from the inlet region toward the downstream compartment is at least partially diverted by the microvalves into the reservoirs as a result of a pressure increase in the microfluidic channel. In some versions, the microvalves are capillary burst valves. A microfluidic chip containing the module and a method of using the module are provided. 1. A pressure regulator module for a chip-based microfluidic platform , comprising:a microfluidic channel comprising an inlet region and an outlet region downstream of the inlet region, said channel for passing flowable material from the inlet region through the outlet region and into a downstream compartment;one or more microvalves fluidly connected to the microfluidic channel and disposed upstream of the outlet region, said microvalves for releasing pressure in the microfluidic channel, wherein each microvalve has an open state permitting flowable material to pass through said microvalve and a closed state preventing flowable material from passing through said microvalve, and wherein each microvalve in its open state diverts flowable material from the microfluidic channel; andone or more reservoirs fluidly connected to the microvalves, for receiving flowable material diverted by the microvalves;wherein a flow of flowable material passing from the inlet region toward the downstream compartment is at least partially diverted by at least one of the microvalves into at least one of the reservoirs as a result of a pressure increase in ...

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Номер записи: 62
21-05-2015 дата публикации

BIOREACTOR SYSTEM AND METHOD FOR CLONING THE PHYSIOLOGICAL STATE OF MICROORGANISMS

Номер: US20150140544A1
Автор: Adamberg Kaarel, Erm Sten, Vilu Raivo
Принадлежит:

Bioreactor system and method for cloning the physiological state of microorganisms comprises in preferred embodiment of a mother-reactor and one or more daughter-reactors with sensors, stirrers, fluid and gas flow channels, scales, pumps, controllers, computer, software, valves and accessory devices. The bioreactors are inter-connected with culture transfer hose. To achieve the method, the mother-reactor is filled with necessary volume, inoculated, stabilised in continuous cultivation, the microbial culture's volume is increased in variable volume cultivation while maintaining constant physiology, microbial culture is transferred from the mother-reactor into the daughter-reactors while maintaining constant physiology, experiment in the daughter reactor follows. After the experiment daughter-reactors are sterilized and rinsed. During the experiment culture volume in the mother-reactor is increased anew, after the experiment in the daughter-reactors is finished another culture transfer follows and next experiment is conducted. This sequence—variable volume cultivation, culture transfer, experiment—is repeated until necessary data has been acquired. 1. A bioreactor system for cloning physiological state of microorganisms is comprising bioreactors equipped with sensors and a stirrer and connected with transfer channels , and fluid inflow and fluid outflow , gas inflow and gas outflow , device to control the volume , pumps , and controllers connected to a mother reactor and daughter reactor(s) , a computer with control software , a temperature maintenance device , an over-pressure valve , extra devices , titrant , and air filter , wherein bioreactors of changeable status are connected into a network of reactors , said network comprising of at least one mother reactor and at least one daughter reactor , wherein the at least one mother reactor has:a transfer channel attached, by means of which the at least one mother reactor is connected to a daughter reactor; an extra ...

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Номер записи: 63
19-05-2016 дата публикации

A Microfluidic Device with a Diffusion Barrier

Номер: US20160137963A1
Автор: Eriksen Johan, Kristensen Anders, Marie Rudolphe
Принадлежит:

The invention provides a microfluidic device for macromolecule amplification by sequential addition of liquid reagents. The device of the invention comprises a chip forming a plurality of reaction chambers each extending between an inlet and an outlet, each inlet being in fluid communication with a common junction via micro channels. To enable amplification of DNA, e.g. by MDA, the device comprises a diffusion barrier at each inlet configured to increase the pressure threshold for a reagent to cross the resistor. The invention further provides a method of mixing liquid reagents by use of the device where single DNA molecules are allowed to cross the diffusion barrier individually. 1. A microfluidic device for macromolecule amplification by sequential addition of liquid reagents , the device comprising , a chip forming a plurality of reaction chambers each extending between an inlet and an outlet , each inlet being in fluid communication with a common junction via micro channels , characterised in that the device comprises a diffusion barrier at each inlet configured to increase the pressure threshold for a reagent to cross the diffusion barrier.2. The device according to claim 1 , where each diffusion barrier is configured to prevent diffusion of macromolecules including genomic DNA and enzymes in or out of the reaction chambers.3. The device according to claim 1 , where the diffusion barrier has a largest dimension in the range of 10-500 nm.4. The device according to claim 1 , where the chamber claim 1 , micro channels claim 1 , and the diffusion barrier are constituted by recesses in a surface of the chip claim 1 , the recesses constituting the diffusion barrier having a lower depth than the recesses constituting the micro channels and chambers.5. The device according to claim 4 , where the recesses are covered by a cover layer bonded to the surface.6. The device according to claim 1 , comprising a plurality of reagent delivery ports where each port has an ...

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Номер записи: 64
19-05-2016 дата публикации

APPARATUS AND METHOD FOR DEHYDRATING BIOLOGICAL MATERIALS

Номер: US20160137997A1
Автор: Durance Timothy D., FU Jun
Принадлежит:

An apparatus and method for microwave vacuum-drying of temperature-sensitive biological materials on a continuous flow-through basis, in which the materials are frozen, ground to frozen particles, dehydrated to a powder, and the powder collected. The apparatus has a microwave generator and waveguide, a freezing chamber with a grinder, a rotatable dehydration chamber in or adjacent to the waveguide, and a powder collector to receive the powdered biological material. The apparatus operates under reduced pressure provided by a vacuum system coupled to the powder collector. 1. An apparatus for continuous throughput dehydration of an aqueous biological material , comprising:(a) a microwave generator;(b) a waveguide to direct microwave radiation from the generator;(c) a freezing chamber for receiving the aqueous biological material and freezing it to form a frozen aqueous biological material;(d) means for feeding the aqueous biological material into the freezing chamber;(e) means for forming a particulate frozen aqueous biological material from the frozen aqueous biological material;(f) a rotatable dehydration chamber in fluid communication with the freezing chamber, the dehydration chamber being positioned in the waveguide to receive microwave radiation produced by the generator, the dehydration chamber having a wall that is transparent to microwave radiation;(g) means for rotating the dehydration chamber;(h) a powder collector in fluid communication with the dehydration chamber;(i) a vacuum system; and(j) means for operatively connecting the vacuum system to the powder collector for applying a vacuum to the freezing chamber, the dehydration chamber and the powder collector whereby the vacuum conveys dehydrated biological material from the dehydration chamber to the powder collector on a continuous throughput basis.2. An apparatus according to claim 1 , further comprising an agitator in the dehydration chamber.3. An apparatus according to claim 1 , further comprising ...

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Номер записи: 65
25-05-2017 дата публикации

Embryo Incubator Incorporating Temperature Control

Номер: US20170145372A1
Автор: Hansen Niels Stengaard, Janssens Ronny
Принадлежит: Kivex Biotec A/S

The invention relates to an incubator having a chamber for cultivating embryos and a gas control system and a temperature control system for providing respectively a predefined atmosphere and a predefined temperature in the incubating chamber. One embodiment relates to an incubator for incubating embryos, comprising one or more incubating chambers adapted to contain the embryo(s), a gas distribution system in fluid communication with the incubating chamber(s), configured to sustain a predefined level of Oand/or COin said incubating chamber(s), and a temperature control system, configured to provide said incubating chamber(s) with a predefined temperature. 1. An incubator for incubating embryos , comprising:one or more incubating chambers adapted to contain one or more embryos;{'sub': 2', '2, 'a gas distribution system in fluid communication with the one or more incubating chambers and configured to sustain a predefined level of Oand/or COin the one or more incubating chambers; and'}a temperature control system configured to provide the one or more incubating chambers with a cyclic temperature profile.2. The incubator according to claim 1 , wherein the cyclic temperature profile simulates a daily biological temperature variation.3. The incubator according to claim 1 , wherein the cyclic temperature profile corresponds to a basal body temperature cycle of a human being.4. (canceled)5. (canceled)624. The incubator according to claim 1 , wherein the cyclic temperature profile is defined by a hour duration claim 1 , a maximum temperature claim 1 , a minimum temperature claim 1 , and four predefined temperature change times claim 1 , and wherein the cyclic temperature profile is repeated automatically.7. (canceled)8. The incubator according to claim 6 , wherein the cyclic temperature profile is repeated automatically for an infinite number of times until a stop is activated claim 6 , or wherein the cyclic temperature profile is repeated automatically for a finite claim 6 ...

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Номер записи: 66
25-05-2017 дата публикации

Method and system for controlling the conversion of lignocellulosic materials

Номер: US20170145374A1
Автор: Eugene Van Rensburg, Josebus Maree Van Zyl, Lee Rybick Lynd, Thomas Michael Harms, Willem Heber van Zyl
Принадлежит: STELLENBOSCH UNIVERSITY

The invention provides a method and system for controlling the conversion of crystalline insoluble cellulose to an organic product in a bioreactor containing crystalline insoluble cellulose and a culture medium. A processor of a computing device receives an input from a sensor in the bioreactor. The input may be a measurement of one or more of concentration, temperature, pH and pressure. The processor calculates conversion of cellulose using the input to provide a total calculated organic product in the bioreactor. The processor receives a further input from a sensor in the bioreactor of the total actual organic product and compares the total calculated organic product and the total actual organic product. The processor then transmits an instruction to an agitator associated with the bioreactor to control agitation of the content of the bioreactor if the total actual organic product is outside a predetermined range of the total calculated organic product.

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Номер записи: 67
25-05-2017 дата публикации

ULTRAHIGH-PRESSURE HOMOGENIZING INTEGRATED DEVICE AND CELL DISRUPTOR

Номер: US20170145376A1
Автор: YU Qian, Yu Xingwen
Принадлежит: Guangzhou Juneng Nano&Bio Technology Co.,Ltd

Disclosed is an ultrahigh-pressure homogenizing integrated device and a cell disruptor. The ultrahigh-pressure homogenizing integrated device includes a long oil cylinder, a main connecting sleeve, a high-pressure cylinder homogenizing main body, an auxiliary connecting sleeve and a short oil cylinder, which are sequentially and coaxially arranged. An upper part of the high-pressure cylinder homogenizing main body is provided with a feeding hole communicated with a high-pressure cavity; and the feeding hole is connected with an integrated feeding device. A pressurizing plunger rod in the high-pressure cavity of the high-pressure cylinder homogenizing main body is connected with a piston rod of the long oil cylinder; and a homogenizing valve arranged in the inner cavity, which is communicated with the high-pressure cavity, of the high-pressure cylinder homogenizing main body, is connected with an ejector rod of the short oil cylinder. 1. An ultrahigh-pressure homogenizing integrated device , comprising a long oil cylinder , a main connecting sleeve , a high-pressure cylinder homogenizing main body , an auxiliary connecting sleeve and a short oil cylinder , wherein a feeding hole communicated with a high-pressure cavity is disposed at an upper part of the high-pressure cylinder homogenizing main body , an integrated feeding device is connected with the feeding hole , the long oil cylinder , the main connecting sleeve , the high-pressure cylinder homogenizing main body , the auxiliary connecting sleeve and the short oil cylinder are sequentially and coaxially arranged , and a pressurizing plunger rod in the high-pressure cavity of the high-pressure cylinder homogenizing main body is connected with a piston rod of the long oil cylinder , a homogenizing valve in an inner cavity , which is communicated with the high-pressure cavity , of the high-pressure cylinder homogenizing main body is connected with an ejector rod of the short oil cylinder.2. The ultrahigh-pressure ...

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Номер записи: 68
02-06-2016 дата публикации

APPARATUS AND PROCESS FOR CONTINUOUS SACCHARIFICATION OF MARINE ALGAE AND CELLULOSIC BIOMASS

Номер: US20160152937A1
Автор: Im Dongjoong, Jeong Dong In, Kim IN Sik, Shin Myung Kyo
Принадлежит:

The present disclosure relates to an apparatus and process for continuous saccharification of marine algae and cellulosic biomass. 1. A apparatus for continuous saccharification of marine algae and cellulosic biomass , the apparatus comprising:a source input unit;a reactor;a reaction unit including a downward reactor and an upward reactor;a reactant liquid discharge unit; anda deposit removal unit.2. The apparatus of claim 1 ,wherein the source includes marine algae including micro-algae, terrestrial cellulosic biomass, or the combination thereof.3. The apparatus of claim 1 ,wherein the marine algae include marine algae containing water or marine algae from which water is removed by a dehydration process.4. The apparatus of claim 1 ,wherein the source input unit further includes an agitator.5. The apparatus of claim 1 ,wherein the reactor comprises a spraying device, a driving unit, and a pressure control device.6. The apparatus of claim 5 ,wherein the spraying device is configured to directly inject high-pressure steam into the reactor.7. The apparatus of claim 1 ,wherein a plurality of the reaction unit is included.8. The apparatus of claim 1 ,wherein the downward reactor and the upward reactor further include a impeller.9. The apparatus of claim 8 ,wherein a slope of the impeller is adjusted depending on a reaction direction of the downward reactor and the upward reactor.10. The apparatus of claim 1 ,wherein the source is mixed with a reaction catalyst in the reactor.11. The apparatus of claim 10 ,wherein the reaction catalyst includes one selected from the group consisting of water, an acid, a base, an enzyme, and the combinations thereof.12. The apparatus of claim 10 ,wherein the reaction catalyst is recycled by collecting through the deposit removal unit.13. The apparatus of claim 1 ,wherein the temperature of the downward reactor and the upward reactor is about from about 50° C. to about 300° C.14. The apparatus of claim 1 ,wherein the reactor, the downward ...

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Номер записи: 69
16-05-2019 дата публикации

METHOD AND SYSTEM FOR A BIOARTIFICIAL ORGAN

Номер: US20190144807A1
Автор: Baker Michael John, Suckow Shelby K.
Принадлежит:

Methods and systems are provided for a stem cell organ device including an array of alternating stem cell channels and fluid channels. In one example, a method may include loading a stem cell channel with stem cells and flowing blood through a fluid channel in order to allow an exchange of molecules between the stem cells and the blood. 1. A method for a stem cell organ device fluidly coupled to a user comprising:entering a blood bypass mode in response to stem cells being less than fully loaded into the stem cell organ device, the blood bypass mode comprising flowing blood through a multi-fluid manifold and back to the user without filtering the blood.2. The method of claim 1 , wherein the blood bypass mode further comprises flowing blood through a first port of the multi-fluid manifold via a variable pump claim 1 , wherein flowing blood through the first port comprises flowing blood through a bypass passage fluidly coupling the stem cell organ device to the user claim 1 , the bypass passage being fluidly separated from a plurality of fluid channels in which the stem cells are arranged.3. The method of claim 2 , further comprising entering a blood mode in response to stem cells being fully loaded into the stem cell organ device claim 2 , wherein blood flows through a second port of the multi-media manifold claim 2 , through the plurality of fluid channels claim 2 , and back to the user without entering the bypass passage.4. The method of claim 3 , further comprising filtering blood in the blood mode claim 3 , wherein each of the plurality of fluid channels comprises a membrane claim 3 , and where the membrane separates the stem cells in a fluid channel from blood so that stems cells and blood do not directly contact one another claim 3 , and where the membrane is shaped to allow nutrients to pass from the stem cells to the blood and for salt claim 3 , water claim 3 , and toxins to pass from the blood to the stem cells.5. The method of claim 2 , further comprising ...

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Номер записи: 70
16-05-2019 дата публикации

Method and Device for Estimation of Alcohol Content in Fermentation or Distillation Vessels

Номер: US20190145947A1
Автор: McCARTHY Wil, Oltyan Chris
Принадлежит:

The subject matter described herein relates to a device and method for estimating the alcohol-by-volume (ABV) of a liquid inside a fermentation or distillation vessel, without opening the vessel or requiring a liquid sample. Other properties of the liquid may also be estimated using this method, by including additional sensors in the device. This method has particular, but not exclusive, application in the home brew, microbrew, home and small batch winemaking, and small-batch distillery industries. 1. A method for estimating the alcohol-by-volume of a liquid in a fermentation or distillation vessel , or in a container of fermented or distilled liquid , comprising:sampling the gas within the vessel or through an aperture within the vessel;waiting until all sensors have settled into a steady state;measuring the temperature, humidity, and alcohol concentration of the gas;comparing the sensor readings against curve fits or machine learning algorithms based on data sets of known ABV, temperature, and humidity, to compute an estimated ABV for the liquid and;either storing the estimated ABV, reporting it to a user, reporting it to an external processor, uploading it to a remote site, or any combination thereof.2. The method of wherein the alcohol concentration of the gas is measured with a semiconductor based alcohol selected from a set that includes but is not limited to MQ-3 compliant sensors.3. The method of wherein the temperature and humidity of the gas are measured with a DHT-22 compliant sensor.4. The method of wherein the output of the alcohol sensor is scaled to match the expectations of the microprocessor.5. The method of claim 1 , wherein the sensitivity of the alcohol sensor is reduced from its native or expected state.6. The method of claim 1 , wherein the sensor is cleaned automatically by means of a cleaning cycle.7. The method of claim 6 , wherein the cleaning cycle is achieved through a voltage increase to a heater circuit.8. The method of claim 1 , ...

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Номер записи: 71
16-05-2019 дата публикации

SYSTEM AND METHOD FOR DETERMINING FILL VOLUME IN A CONTAINER

Номер: US20190145955A1
Автор: Bachur, Foley, Jr. Nicholas R., JR. Timothy G.
Принадлежит: BECTON DICKINSON AND COMPANY

A system and method for detecting a pathogen in a sample is provided, the system capable of measuring the volume of a sample in a container through the use of various measurement technologies, thereby ensuring that a user is aware of volumes not meeting specification and/or allowing correction of results to account for the out-of-specification sample. 125-. (canceled)26. A system for detecting a pathogen in a sample , comprising a plurality of sample containers comprising a growth medium and adapted to receive a biological fluid sample; an incubation and measurement module comprising a plurality of individual openings , each opening adapted to receive one of the plurality of sample containers; a weight variation sensor , separate from or part of said incubation and measurement module , the weight variation sensor adapted to determine a sample volume in said sample containers; and one or more interfaces adapted to perform one or more tasks selected from the group consisting of: (a) notifying a user if said sample containers are not within predetermined sample volume specifications , (b) accepting instructions on subsequent handling of said sample containers that are not within predetermined sample volume specifications , and (c) prompting user to place said sample container into a test rack of said incubation and measurement module if sample volume is within sample volume specification wherein the weight variation sensor: i) determines a prior container weight , wherein the prior container weight is a container weight before the sample is added thereto; ii) stores the prior container weight; iii) determines a sample container weight after the sample is added to the sample container; iv) subtracts the prior container weight from the sample container weight to obtain a weight difference; and iv) calculates a sample volume from the weight difference.27. The system of wherein said weight variation sensor is part of said incubation and measurement module.28. The system of ...

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Номер записи: 72
17-06-2021 дата публикации

CELL COMPRESSION DEVICE

Номер: US20210179997A1
Автор: LI Qingsen
Принадлежит: FONDAZIONE ISTITUTO FIRC DI ONCOLOGIA MOLECOLARE (IFOM)

A cell compression device having a main body including a central internal cavity with a symmetry longitudinal axis and a lower opening aligned with the axis, and a deformable membrane having a piston attached thereto is disclosed. The membrane is clamped to the main body at the lower opening to close the central internal cavity forming a variable pressure chamber. The attached piston is located outside the variable pressure chamber and the membrane being clamped to the main body in correspondence with clamping points. When the deformable membrane is clamped to the main body, the membrane is in a relaxed configuration, wherein the distance of two opposing clamping points is lower than the length of the membrane between the two opposing clamping points, and a symmetry longitudinal axis of the main portion of the piston coincides with the symmetry longitudinal axis of the central internal cavity. 1. A cell compression device for applying dynamic compression stress to cells (C) , comprising:a main body having a central internal cavity with a symmetry longitudinal axis (A) and a lower opening aligned with said axis (A),a deformable membrane having a piston attached thereto, the piston comprising a main portion with a symmetry longitudinal axis (B), andclamping means to clamp the membrane to the main body at the lower opening to close the central internal cavity so that the closed central internal cavity forms a variable pressure chamber, the attached piston being located outside said variable pressure chamber and the membrane being clamped to the main body in correspondence of clamping points,wherein in an assembled configuration, when the deformable membrane is clamped to the main body, said membrane is in a relaxed configuration, wherein the distance of two opposing clamping points is lower than the length of the membrane between said two opposing clamping points, and the symmetry longitudinal axis (B) of the main portion of the piston coincides with the symmetry ...

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Номер записи: 73
31-05-2018 дата публикации

PORTABLE DEVICE FOR CONTROLLING AN ALCOHOLIC BEVERAGE THROUGH A CONTAINER, A SYSTEM AND A METHOD ASSOCIATED THERETO

Номер: US20180149592A1
Автор: Bruneel Delhaye Caroline, BRUNEEL Jean-Luc, Brunerie Pascal, Fil Benoit, Gouret Katia, Guillaume Francois, Verger Stephane
Принадлежит:

The present disclosure provides a portable device for controlling an alcoholic beverage that includes a single light source emitting a monochromatic excitation light beam of a wavelength between 350 and 650 nanometers, a beam splitter to reflect the light beam, a focus and collection lens, and a positioning device to orient the light beam along a direction substantially normal to an outer surface of the container. The positioning device positions the outer surface of the container at a predetermined distance from the focus lens. The device includes a filtering device for filtering fluorescence radiation captured by the focus lens to eliminate wavelengths shorter than or equal to the wavelength of the light beam. A spectrometer module produces a signal corresponding to the measured spectrum of the fluorescence radiation and an analysis module compares the measured spectrum to a reference spectrum. 1. A portable device for controlling an alcoholic beverage in an at least partially transparent container , the portable device comprising:a single light source emitting a monochromatic excitation light beam having a wavelength between 350 and 650 nanometers;a beam splitter oriented at 45° with respect to the direction of emission of the light source to reflect the excitation light beam;a focus and collection lens;a positioning device that orients the excitation light beam emitted from the light source along a direction normal to an outer surface of the container, wherein the positioning device is operable to position the outer surface of the container at a predetermined distance from the focus and collection lens such that the excitation light beam is focused within the container and the distance from the wall of the container is less than 1 millimeter, wherein the positioning device includes a through opening for passage of the excitation light beam;at least one filtering device for filtering fluorescence radiation captured by the focus lens and transmitted by the beam ...

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Номер записи: 74
23-05-2019 дата публикации

MULTIWELL PLATE WITH INTEGRATED STIRRING MECHANISM

Номер: US20190153376A1
Автор: Ahrar Siavash, Duncan Philip N., Hui Elliot En-Yu, Nguyen Transon V., Werner Erik Morgan
Принадлежит:

This invention describes a design for a multiwell plate that contains integrated pumps that are used to stir each well of the plate. The device employs microfluidic logic technology to drive each peristaltic pump. This enables the plates to run autonomously, requiring only a static vacuum supply for power. The devices are entirely constructed out of low-cost polymers, with no electronics, and yet contains simple digital logic circuits to control the pumps. A stack of these plates may be run continuously in a standard cell culture incubator, allowing high-throughput culture of organoids. 1500. An integrated multiwell stirring plate () comprising:{'b': '501', 'a. a plate body ();'}{'b': 502', '501, 'b. a plurality of wells () embedded within the plate body (); and'}{'b': '510', 'claim-text': [{'b': '504', 'i. a pump inlet ();'}, {'b': 506', '502, 'ii. a pump outlet (), fluidly connected with one of the wells (); and'}, {'b': 508', '504', '506, 'iii. a fluid channel (), fluidly connecting the pump in line between the pump inlet () and the pump outlet ();'}], 'c. a plurality of pneumatic, peristaltic pumps (), each pump comprising{'b': 510', '508', '506', '511', '502', '511', '512', '502, 'wherein the pump () is configured to pump a fluid through the fluid channel () and out of the pump outlet () so as to produce a fluid jet () into the well (), and wherein each jet () is configured to impart a convective flow () of the fluid within the well (); and'}{'b': 540', '510, 'd. one or more microfluidic pneumatic control mechanisms () configured to control the pumps ();'}{'b': 510', '540', '501, 'wherein both the peristaltic pumps () and the control mechanisms () are embedded and integrated within the plate body ().'}2511. The multiwell stirring plate of claim 1 , wherein the jet () is angled to agitate the fluid in a flow pattern.3. The multiwell stirring plate of claim 2 , wherein the flow pattern is a rotational flow pattern.4. The multiwell stirring plate of claim 2 , ...

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Номер записи: 75
08-06-2017 дата публикации

CELL CONCENTRATION ADJUSTMENT DEVICE, AND AUTOMATIC SUBCULTURE SYSTEM USING SAME

Номер: US20170159003A1
Автор: Aso Sadamitsu, Imai Kazumichi, Kawarai Masako, SAKURAI Toshinari, SHIMASE Akihiro, Takada Eiichiro
Принадлежит: HITACHI HIGH-TECHNOLOGIES CORPORATION

A device which automatically performs a step in which expanded and cultured cells are diluted to a desired cell concentration and re-inoculated using a cell-concentration adjustment device having an inlet for taking in a cell suspension; an outlet for discharging a diluted cell suspension; and a flow path which is provided between the inlet and the outlet and is capable of holding a cell suspension, the flow path being provided with: a liquid delivery pump for causing a cell suspension inside to flow; a cell-concentration measurement instrument for collecting data related to a cell concentration per unit amount of the cell suspension; and a dilution-liquid container for holding a dilution liquid which is supplied to the flow path to dilute the cell suspension. The device further includes a control unit for controlling at least the liquid delivery pump on the basis of the data obtained by the cell-concentration measurement instrument, wherein the control unit determines, on the basis of the data obtained by the cell-concentration measurement instrument, an amount of the dilution liquid required to bring the cell concentration to the desired concentration, and drives the liquid delivery pump so as to take in the required amount of the dilution liquid into the flow path and mix the cell suspension and the dilution liquid. 2. The cell-concentration adjustment device according to claim 1 , wherein at least a part of the flow path provided between the inlet and the outlet forms a circulation flow path claim 1 , the liquid delivery pump and the cell-concentration measurement instrument are provided in the circulation flow path claim 1 , and the control unit drives the liquid delivery pump to cause the cell suspension and the dilution liquid to repeatedly flow in the circulation flow path until a variation in the data obtained from the cell-concentration measurement instrument becomes a value within a predetermined range claim 1 , to thereby mix the cell suspension and the ...

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Номер записи: 76
28-08-2014 дата публикации

METHODS FOR DETECTING FILM FORMATION POTENTIAL IN FOOD PRODUCTS

Номер: US20140244201A1
Автор: GNANASEKHARAN Vivek, HIGGINS Leigh Anne
Принадлежит: NESTEC S.A.

The present disclosure provides methods for determining the film forming potential of food products. In a general embodiment, a method for determining the film forming potential of a food product is provided and includes providing a temperature-control device having a processor and a computer readable medium storing instructions which, when executed, cause the processor to cycle an interior temperature of the temperature-control device between different temperatures in a predetermined amount of time. The methods further include placing a packaged food product in the temperature-control device, and causing the processor to execute the stored instructions. 1. A method for testing at least one food product for film formation potential , the method comprising:providing a temperature-control device comprising a processor and a computer readable medium storing instructions which, when executed, cause the processor to cycle an interior temperature of the temperature-control device between at least three temperatures in a predetermined amount of time;placing a packaged food product in the temperature-control device; andcausing the processor to execute the stored instructions,determining an amount of film formed on a surface selected from the group consisting of the food product, an interior lid on the packaged food product, an interior of the packaging, and combinations thereof; andwherein the stored instructions, when executed, cause the processor to cycle the interior temperature of the temperature-control device at least once.2. The method according to further comprising programming the computer readable medium to contain the stored instructions.3. The method according to wherein the stored instructions claim 1 , when executed claim 1 , cause the processor to cycle the interior temperature of the temperature-control device more than once.4. The method according to further comprising shearing the packaged food product before packaging.5. The method according to wherein a ...

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Номер записи: 77
15-06-2017 дата публикации

SINGLE-USE BIOREACTOR SENSOR INTERFACE

Номер: US20170166852A1
Автор: Feng Chang-Dong
Принадлежит:

A bioreactor sensing system is presented. The bioreactor sensing system comprises a bioreaction vessel. The bioreaction vessel comprises an aperture configured to provide an interface for monitoring contents within the bioreaction vessel. The bioreaction vessel also comprises a port coupled to the bioreaction vessel proximate the aperture. The bioreactor sensing system also comprises a sensing device disposed at least partially within the port such that a sensing element is exposed to the contents. The bioreactor sensing system also comprises an external seal configured to be applied over a portion of the sensing device and a portion of the port. 1. A bioreactor sensing system comprising: an aperture configured to provide an interface for monitoring contents within the bioreaction vessel; and', 'a port coupled to the bioreaction vessel proximate the aperture;, 'a bioreaction vessel comprisinga sensing device disposed at least partially within the port such that a sensing element is exposed to the contents; andan external seal configured to be applied over a portion of the sensing device and a portion of the port.2. The system of claim 1 , wherein the external seal comprises elastomeric tubing.3. The system of claim 1 , wherein the sensing device comprises a sensor feature claim 1 , the port comprises a port feature claim 1 , and wherein the sensor feature claim 1 , and the port feature comprise an interface claim 1 , and wherein the external seal fluidically seals the interface.4. The system of claim 3 , wherein the sensing element comprises a sensor hose barb.5. The system of claim 4 , wherein the port feature comprises a port hose barb claim 4 , and wherein the port hose barb and the sensor hose barb have opposing directionalities.6. A method of sealably coupling a sensor to a bioreactor claim 4 , comprising:locating a port on the bioreactor, that is configured to receive a sensing element of the sensor;positioning the sensor within the port such that the sensing ...

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Номер записи: 78
30-05-2019 дата публикации

Pure algae growth system and method

Номер: US20190161714A1
Автор: Martin L. Johnson
Принадлежит: Siftex Equipment Company Inc

A tank receives, mixes, and dispenses input materials including algae feed stock, a micro-nutrients, cleaning solution, a sterile gas, a heating/cooling fluid, carbon dioxide, and raw water with a water treatment system. A plurality of output stations include a vent to atmosphere, a heating/cooling media return, a cleaning disposal, and an algae concentrate/product. The vent to atmosphere is coupled to the tank. The heating/cooling media return is coupled to the heating/cooling media supply through the tank. The cleaning solution disposal station is coupled to the tank with a pump followed by a nano bubbler and an algae growing system. An algae concentrate/product station is coupled to the tank. An algae dewatering system is intermediate the tank and the algae concentrate/product. A water return line couples the algae dewatering system and the water treatment system.

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Номер записи: 79
21-05-2020 дата публикации

MONITORING SYSTEM FOR WINEMAKING

Номер: US20200157479A1
Автор: Botas Bilro Lúcia Maria, Estima da Costa Pedro Miguel, Gonçalves Fábio Patrício Domingues, Nunes Nogueira Rogério, Ventura de Sousa e Carvalho Pereira Ricardo José
Принадлежит:

Aspects of a monitoring method and system for winemaking are provided. The system comprises at least two sensors, a controller, a wireless transmitter and a wireless receiver. One sensor measures a wine parameter, while the other sensor measures an ambient condition. The controller is able to generate an alarm based on the wine parameter and the ambient condition. The wireless transmitter is able to communicate the wine parameter and the ambient condition to a remote database. The wireless receiver is able to receive a modification to the firmware. 1. A system for monitoring winemaking comprising:a first sensor operable to measure a wine parameter;a second sensor operable to measure an ambient condition;a controller operable according to firmware stored in a memory, wherein the controller is operable to generate an alarm according to the wine parameter and the ambient condition;a wireless transmitter operable to communicate the wine parameter and the ambient condition to a remote database; anda wireless receiver operable to receive a modification to the firmware.2. The system according to claim 1 , wherein the wine parameter is one of a pressure claim 1 , a temperature claim 1 , a density claim 1 , a level claim 1 , a turbidity claim 1 , a color claim 1 , a pH and an alcohol content.3. The system according to claim 1 , wherein the ambient condition is measured by a temperature probe that is external to a container that comprises the wine.4. The system according to claim 1 , wherein the ambient condition is measured by a hygrometer.5. The system according to claim 1 , wherein the ambient condition is measured by a lux sensor.6. The system according to claim 1 , wherein the system comprises an accelerometer.7. The system according to claim 6 , wherein the accelerometer is operable to calibrate an orientation of the system.8. The system according to claim 6 , wherein the accelerometer is operable to remotely indicate that the system is askew.9. The system according to ...

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Номер записи: 80
21-05-2020 дата публикации

FERMENTATION MONITORING SYSTEM

Номер: US20200158602A1
Автор: Botas Bilro Lúcia Maria, Estima da Costa Pedro Miguel, Gonçalves Fábio Patrício Domingues, Nunes Nogueira Rogério, Ventura de Sousa e Carvalho Pereira Ricardo José
Принадлежит:

Aspects of a monitoring system for winemaking are provided. 1. A system is provided, substantially as shown and described shown and described with respect to for monitoring winemaking. Limitations and disadvantages of conventional and traditional monitoring systems for winemaking will become apparent to one of skill in the art, through comparison of such systems with some aspects of the present invention as set forth in the remainder of the present application with reference to the drawings.Monitoring systems for winemaking substantially as shown in and/or described in connection with at least one of the figures, as set forth more completely in the claims.These and other advantages, aspects and novel features of the present invention, as well as details of an illustrated embodiment thereof, will be more fully understood from the following description and drawings.This disclosure describes systems for monitoring a wine fermentation or other similar process. Many facets of wine change during the fermentation process. Parameters (e.g., pressure, temperature, density, level, turbidity, color, pH, alcohol content) may be automatically captured continuously or otherwise over time and wirelessly transferred to a database for analysis and storage. Thresholds may be set such that a winemaker can be notified when the monitored parameters are out of selected or desired range.illustrates components of a sensor device in accordance with an example implementation of this disclosure. The depicted components of the sensor device include a controller , an on/off switch , an alarm , a power supply , one or more sensor(s) , and a transceiver .In operation, the sensor device is coupled to a wine barrel or vat during a winemaking process. The temperature probe measures the temperature of the wine, and the one or more sensor(s) measure additional parameters (e.g., pressure, density, level, turbidity, color, pH, alcohol content) of the wine. These measurements are conveyed to the ...

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Номер записи: 81
21-06-2018 дата публикации

Liquid Flow-Circulation Chamber, Cell Concentration Device, and Cell Concentration System

Номер: US20180171278A1
Автор: Hashiba Shuhei, IGARASHI Yumiko, KATO Midori, KIYAMA Masaharu
Принадлежит:

The present invention is directed to provide cell concentration devices and cell concentration systems having a novel liquid flow-circulation chamber. It is a cell concentration device including a liquid flow-circulation chamber, the liquid flow-circulation chamber having a first tube and a second tube, the first tube and the second tube being connected with each other via a liquid delivery tube outside the liquid flow-circulation chamber, the liquid delivery tube including a liquid delivery pump and a cell concentration module, the liquid delivery pump being capable of feeding a liquid through the liquid delivery pump in both directions, the first tube and the second tube each having an open end in a liquid in the liquid flow-circulation chamber, the first tube having an opening in a gas in the liquid flow-circulation chamber, and the liquid being a suspension of cells. 1. A liquid flow-circulation chamber comprising a first tube and a second tube , the liquid flow-circulation chamber being hermetically closed ,the first tube and the second tube being connected with each other via a liquid delivery tube outside the liquid flow-circulation chamber,the liquid delivery tube comprising a liquid delivery pump capable of feeding a liquid through the liquid delivery pump in both directions,the first tube and the second tube each having an open end in a liquid in the liquid flow-circulation chamber, andthe first tube having an opening in a gas in the liquid flow-circulation chamber.2. The liquid flow-circulation chamber according to claim 1 , further comprising a third tube connected to a gas bag.3. The liquid flow-circulation chamber according to claim 1 , wherein an opening area of the opening is larger than a cross-sectional area of the first tube.4. The liquid flow-circulation chamber according to claim 1 , wherein the liquid contains cells.5. A cell concentration device comprising a liquid flow-circulation chamber claim 1 ,the liquid flow-circulation chamber having a ...

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Номер записи: 82
06-06-2019 дата публикации

WINE PROBE

Номер: US20190170719A1
Автор: GUILLEBASTRE Bastien, MUNOZ Fabien
Принадлежит: OENONEO

A wine probe includes a sealed body configured to be immersed in wine and defining a through cavity of the body. The probe includes at least one light source positioned on a first sidewall of the cavity configured to consecutively emit at least two radiations in at least two different wavelengths. The probe also includes at least one light sensor positioned on a second side wall opposite the first, facing the light source and configured to measure at least two light intensities from the radiations which have passed through the cavity. The probe also includes a control device configured to initiate emission of the radiations and to collect the measurements made by the sensor. The control device is configured to transmit data relative to the analysed wine to a readout from the measurements. Also, a method measures quantities relative to a wine by the probe. 111-. (canceled)12. Wine probe comprising:a sealed body configured to be immersed in wine and defining a through cavity of the sealed body,a cover closing the sealed body,at least one light source configured to consecutively emit at least two radiations in at least two different wavelengths, the at least one light source being positioned on a first side wall of the through cavity defined in the sealed body,at least one light sensor positioned on a second side wall of the through cavity opposite the first side wall, the at least one light sensor being placed facing the at least one light source, the at least one light sensor being configured to measure at least two light intensities from the at least two radiations emitted by the at least one light source which have passed through the through cavity,a control device configured to initiate emission of the at least two radiations and to collect the light intensity measurements made by the at least one light sensor, the control device being configured to transmit data relative to an analysed wine to a readout from the at least two light intensity measurements, the ...

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Номер записи: 83
04-06-2020 дата публикации

APPARATUS FOR SUPPLYING GRAIN AND FLOUR TO CONTAINER OF FOOD PROCESSING MACHINE

Номер: US20200170261A1
Автор: NOGUCHI Akinori, UENO Maya
Принадлежит: SODICK CO., LTD.

A grain and flour supply apparatus is provided, including a holding bin provided above a container and having an exhaust port from which gas is discharged, an introduction port into which grain and flour are introduced, and a supply port which supplies the grain and flour to the container; a first vacuum breaker valve opening and closing the exhaust port; a second vacuum breaker valve opening and closing the introduction port; a third vacuum breaker valve opening and closing the supply port; a temperature sensor measuring the temperature in the holding bin; a vacuum device drawing a vacuum in the holding bin; and a control device configured to control the first vacuum breaker valve, the second vacuum breaker valve, the third vacuum breaker valve, and the vacuum device, and set with a target temperature. 1. A grain and flour supply apparatus for supplying grain and flour to a container of a food processing machine , comprising:a holding bin provided above the container and comprising an exhaust port from which gas is discharged, an introduction port into which the grain and flour are introduced, and a supply port which supplies the grain and flour to the container;a first vacuum breaker valve opening and closing the exhaust port;a second vacuum breaker valve opening and closing the introduction port;a third vacuum breaker valve opening and closing the supply port;a temperature sensor measuring a temperature in the holding bin and outputting a measured temperature;a vacuum device comprising one or more vacuum pumps connected to the exhaust port via the first vacuum breaker valve, and drawing a vacuum in the holding bin; anda control device configured to control the first vacuum breaker valve, the second vacuum breaker valve, the third vacuum breaker valve, and the vacuum device, and set with a target temperature,wherein the control device operates the vacuum device in a state where the first vacuum breaker valve is opened and the second vacuum breaker valve and the ...

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Номер записи: 84
28-06-2018 дата публикации

SENSOR INTERFACE FOR SINGLE-USE CONTAINERS

Номер: US20180179486A1
Автор: Fadell Paul R., Price Joshua M.
Принадлежит:

A single-use sensor interface for coupling a single-use container to a re-usable sensing instrument is provided. The single-use sensor interface includes a polymeric flange couplable to a wall of the single-use container. The polymeric flange has a sidewall and an interface portion configured to receive a deflectable polymeric diaphragm. A deflectable polymeric diaphragm is coupled to the interface portion of the polymeric flange. An instrument coupling portion is sealingly coupled to the polymeric flange. The instrument coupling portion is configured to couple to the re-usable sensing instrument. Another single-use sensor interface for coupling a single-use container to a re-usable sensing instrument includes a polymeric hose adapter and at least one instrument attachment portion. The polymeric hose adapter portion has at least one hose barb configured to retain a hose slid thereover. The hose adapter portion has at least one interface portion that is configured to receive a deflectable polymeric diaphragm. A deflectable polymeric diaphragm is coupled to the interface portion. At least one instrument attachment portion is coupled to the hose adapter portion. The at least one instrument attachment portion has a cylindrical sidewall. 1. A single-use sensor interface for coupling a single-use container to a re-usable sensing instrument , the interface comprising:a polymeric flange couplable to a wall of the single-use container, the polymeric flange having a sidewall and an interface portion configured to receive a deflectable polymeric diaphragm;a deflectable polymeric diaphragm coupled to the interface portion of the polymeric flange;an instrument coupling portion sealingly coupled to the polymeric flange, the instrument coupling portion being configured to couple to the re-usable sensing instrument.2. single-use sensor interface of claim 1 , wherein the instrument coupling portion releasably couples to the polymeric flange using a clamp.3. The single-use sensor ...

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Номер записи: 85
18-09-2014 дата публикации

DOUGH-MIXING CONTROL METHOD AND DEVICE CAPABLE OF DETERMINING DOUGH GLUTEN DEVELOPMENT

Номер: US20140272024A1
Автор: CHEN Shuang-Yuan
Принадлежит: SINMAG EQUIPMENT CORPORATION

A dough-mixing control device capable of determining dough gluten development is mounted on a dough mixer and has a controller, a power sensor, a temperature sensor, an output module and an operation module. The controller is electrically connected to the power sensor, temperature sensor, output module and operation module. Each parameter and determination condition inputted through the operation module is transmitted to the controller. A dough gluten development determination procedure built in the controller controls temperature and gluten development of mixed dough and performs statistical calculation according to the inputted parameters and determination conditions so as to acquire the optimal gluten development of dough. Accordingly, drawbacks such as high human production cost and unstable dough quality of conventional dough mixing that relies on human experience to determine dough gluten development can be resolved. 1. A dough-mixing control method capable of determining dough gluten development , comprising:a dough gluten development determination step determining if a moving average ratio of average current or power has reached its maximum where the average current or power is consumed by a dough mixer while mixing a piece of dough, and if positive, further determining that an optimal condition of dough gluten of the piece of dough has been reached; anda statistical production control step recording or calculating an optimal total mixing time and an optimal total mixing energy associated with a dough recipe, wherein the optimal total mixing time is equal to a calculated sum of multiple mixing periods corresponding to multiple mixing speed stages in the dough mixing process, and the optimal total mixing energy is equal to a sum of power consumed in the mixing speed stages in the dough mixing process.7. The method as claimed in claim 1 , whereineach of the optimal total mixing time and the optimal total mixing energy has a range factor serving as a tolerance ...

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Номер записи: 86
04-06-2020 дата публикации

GAS-FED FERMENTATION REACTORS, SYSTEMS AND PROCESSES

Номер: US20200172853A1
Автор: AYLEN Graham Ian, Johannessen Arild, NGUYEN Luan Thanh, Silverman Joshua A.
Принадлежит:

Reactors, systems and processes for the production of biomass by culturing microorganisms in aqueous liquid culture medium circulating inner loop reactor which utilize nonvertical pressure reduction zones are described. Recovery and processing of the culture microorganisms to obtain products, such as proteins or hydrocarbons is described. 1. A system for stimulating production of biomass comprising: a gas/liquid separation vessel for separating a multi-phase mixture of a gas and a liquid culture medium into a gas phase and a liquid phase, the gas/liquid separation vessel including an outlet and an inlet;', 'a loop section including an inlet in fluid communication with the outlet of the gas/liquid separation vessel and an outlet in fluid communication with the inlet of the gas/liquid separation vessel, the loop section including a portion of the loop section between the inlet of the loop section and the outlet of the loop section slopes upward; and', 'a first non-vertical pressure reduction zone including an eccentric expander, the first non-vertical pressure reduction zone located between the inlet of the loop section and the outlet of the loop section., 'a loop reactor, the loop reactor including2. The system of claim 1 , wherein the eccentric expander includes a pipe section having a constant inner diameter.3. The system of claim 2 , wherein the eccentric expander includes an eccentric expander section connected to the pipe section.4. The system of claim 1 , wherein the eccentric expander includes an eccentric expander section having a first end having a first inner diameter and a second end having a second inner diameter claim 1 , the second inner diameter being larger than the first inner diameter.5. The system of claim 3 , wherein the eccentric expander section has a first end having a first inner diameter and a second end having a second inner diameter claim 3 , the second inner diameter being larger than the first inner diameter claim 3 , and the first inner ...

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Номер записи: 87
15-07-2021 дата публикации

FACTORY-ON-A-CHIP FOR PRODUCTION OF BIOLOGICALLY DERIVED MEDICINES/BIOPHARMACEUTICALS/BIOLOGICS/ BIOTHERAPEUTICS

Номер: US20210214664A1
Автор: AL-ADHAMI Mustafa, ANDAR ABHAY, Kostov Yordan, Rao Govind
Принадлежит:

The present invention provides for a fully integrated microfluidic system capable of producing single-dose amounts of biotherapeutics at the point-of-care wherein protein production, purification and product harvest are all integrated as a single microfluidic device which is portable and capable of continuous-flow production of biotherapeutics at the microscale using a cell-free reaction system. 1. A factory-on-a-chip microfluidic device comprising:(i) a microfluidic bioreactor unit equipped with a continuous collection channel for a synthesizing a crude protein in a reaction within the microfluidic bioreactor unit;(ii) a microfluidic mixer/de-bubbler unit communicatively connected to the microfluidic bioreactor unit to dilute the crude protein and remove any air bubbles during mixing; and(iii) a microfluidic purification unit communicatively connected to the microfluidic mixer/de-bubbler unit comprising at least one purification column capturing the crude protein and providing a purified protein.2. The factory-on-a-chip microfluidic device according to claim 1 , further comprising at least one process analytical technology (PAT) sensor for monitoring pH claim 1 , pressure claim 1 , temperature claim 1 , dissolved-oxygen claim 1 , redox conditions claim 1 , ionic strength claim 1 , UV-Vis absorbance claim 1 , fluorescence claim 1 , light scatter claim 1 , and/or circular dichroism conditions during the reaction claim 1 , purification and/or analysis of the crude and/or purified protein claim 1 , wherein the at least one sensor is communicatively connected to the a microfluidic bioreactor unit and/or microfluidic purification unit.3. The factory-on-a-chip microfluidic device according to claim 1 , wherein the mixer/de-bubbler comprises a porous membrane to eliminate bubbles.4. The factory-on-a-chip microfluidic device according to claim 1 , wherein the units (i) claim 1 , (ii) claim 1 , and (iii) are stacked together to form a single unit.5. The factory-on-a-chip ...

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Номер записи: 88
15-07-2021 дата публикации

BIOENVIRONMENTAL SIMULATION DEVICE AND METHOD

Номер: US20210214667A1
Автор: KIM Dong Ki, Kim Ji Eun, KIM Yon Su, Kim Yong Chul, LEE Sa Ram, YANG Seung Hee, Yu Mi Yeon
Принадлежит:

The bioenvironmental simulation device according to an embodiment of the present invention includes at least one mounting unit on which cells to be measured are placed, a rotational force application unit configured to rotate the mounting unit so as to apply a rotational force to the cells to be measured placed on the mounting unit, and a culture liquid flow device through which a culture liquid flows across the mounting unit, wherein the culture liquid flows by the culture liquid flow device so as to apply a shear force to the cells to be measured. 1. A bioenvironmental simulation device , comprising:at least one mounting unit on which cells to be measured are placed;a rotational force application unit configured to rotate the mounting unit so as to apply a rotational force to the cells to be measured placed on the mounting unit; anda culture liquid flow device through which a culture liquid flows across the mounting unit, wherein the culture liquid flows by the culture liquid flow device so as to apply a shear force to the cells to be measured.2. The bioenvironmental simulation device according to claim 1 , wherein the at least one mounting unit comprises:a first mounting part which has a first cell placing surface on which a first cell to be measured is placed on one surface of a separation membrane, and forms a first space through which a first culture liquid passes with facing the first cell placing surface;a second mounting part which has a second cell placing surface on which a second cell to be measured interacting with the first cell to be measured is placed on the other surface of the separation membrane, and forms a second space through which a second culture liquid passes with facing the second cell placing surface, wherein the second space is partitioned from the first space by the separation membrane.3. The bioenvironmental simulation device according to claim 2 , wherein the first and second culture liquids are configured to pass through the first and ...

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Номер записи: 89
07-07-2016 дата публикации

DEVICE FOR IN-VITRO MODELLING IN-VIVO TISSUES OF ORGANS

Номер: US20160194588A1
Автор: FELDER Marcel, Geiser Thomas, GUENAT Olivier Thierry, STUCKI Andreas, STUCKI Janick Daniel
Принадлежит:

A device () for in-vitro modelling in-vivo tissues of organs, comprises: a first body portion () with at least one access chamber (), a second body portion () with at least one culturing chamber (), and a culturing membrane () dividing the at least one access chamber () from the culturing chamber (). The device further comprises a third body portion () with at least one actuation chamber () having at least one limitation cavity, and an actuation membrane () dividing the at least one culturing chamber () from the at least one actuation chamber (). With the device according to the invention a robust actuation system can be provided that does not depend on the mechanical properties of the actuation membrane material, nor on the pressure, and that allows to mimic three-dimensional deformations of the tissue, in particular of lung alveoli. 1. A device for in-vitro modelling in-vivo tissues of organs , the device comprising:a first body portion with at least one access chamber;a second body portion with at least one culturing chamber;a culturing membrane dividing the at least one access chamber from the culturing chamber;a third body portion with at least one actuation chamber having at least one limitation cavity; andan actuation membrane dividing the at least one culturing chamber from the at least one actuation chamber.2. The device according to claim 1 , wherein a volume of the at least one limitation cavity of the at least one actuation chamber is adjusted to correspond to a predefined deflection of the culturing membrane into or from the at least one culturing chamber.3. The device according to claim 1 , wherein the at least one actuation chamber is connected to a deflection actuation channel.4. The device according to claim 1 , wherein the at least one actuation chamber is connected to a deflection actuation port being connected to a pressure application means for adjusting the pressure inside the at least one limitation cavity of the at least one actuation chamber ...

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Номер записи: 90
05-07-2018 дата публикации

A BIOREACTOR SYSTEM AND METHOD THEREOF

Номер: US20180187139A1
Автор: Patel Ravindrakumar Dhirubhai
Принадлежит:

The present invention relates to bioreactor system and method thereof wherein support matrix () comprises at last one central shaft and plurality of peripheral shaft being radially surrounds central shaft. Arrays of discs () are mounted along the shaft by defining interspatial vicinities between two successive plates. Thus, discs mounted on peripheral shafts are rotated within the interspatial vicinity of discs of central shaft to ensures sufficient mixing and avoid stagnant fluidic zones which is created when discs are mounted closely apart from each other on shafts. Further, plurality of deflector vanes that are axially provided along the length of the central shaft to redirect substantially co-axial direction fluid flow into interior of culture vessel and more specifically towards the central axis. Thus, bioreactor system provides scalable and disposable bioreactor with efficient mixing and homogeneous conditions and thereby supports high density growth and maintenance of cells and other biological material. 1. A bioreactor system for processing , propagating , culturing , entrapping or encapsulating the biological material , cells , chemicals or enzymes and comprising:{'b': 1', '1', '1', '14', '15, 'i': a', 'b, 'at least one culture vessel () wherein process for culturing cells takes place arranged to contain fluid medium having an inlet port () and an outlet port () and contain at least one fluid IN/Out conduits () for the purpose of supplying fluid therein and discharging fluid therefrom after completion of culturing process to maintain desired metabolic state of the culture process including inoculation conduit and sampling conduit and contains at least one sensing element () for temperature, pressure, pH, oxygen, carbon dioxide and other metabolites important to be measured and controlled during process;'}{'b': 2', '1', '2', '9', '1', '6', '9', '6', '10', '1', '9', '11', '9', '10', '11', '11', '1', '12', '1', '11', '9', '10', '8', '13', '9', '10', '16', '9', ...

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Номер записи: 91
07-07-2016 дата публикации

METHOD AND AN APPARATUS FOR IDENTIFYING A CONTENT PROPERTY OF FOODSTUFF

Номер: US20160195469A1
Автор: Manning Sean, Richardson Andrew Callum, Rowland Kristopher John
Принадлежит:

The present invention relate to an apparatus for processing of foodstuff. The apparatus comprises an element for processing of the foodstuff. The apparatus further comprises a light source that is arranged to emit light having at least two wavelengths or wavelength ranges, the light source further being arranged to direct the light to a surface of at least the foodstuff, and a detector positioned to detect at least a portion of light reflected from the surface of the foodstuff, and arranged to generate an output indicative of the detected intensity. In addition, the apparatus comprises a component for identifying a content property of the foodstuff, the component being arranged to determine a relation between a generated output for one of the at least two wavelengths or wavelength ranges and a generated output for the other or another one of the at least two wavelengths or wavelength ranges, the component further being arranged to compare the determined relation with predetermined data to generate information indicative of the content property of the foodstuff. The apparatus is arranged such that processing of the foodstuff is controlled using the information indicative of the content property of the foodstuff. 1. An apparatus for processing of foodstuff , the apparatus comprising:an element for processing the foodstuff;a light source for emitting light and positioned to direct the light to a surface of at least the foodstuff, the emitted light having at least two wavelengths or wavelength ranges;a detector positioned to detect at least a portion of the emitted light that is reflected from the surface of the foodstuff, the detector being arranged to generate an output indicative of detected light intensity;a component for identifying a content property of the foodstuff and being arranged to determine a relation between a generated output for one of the at least two wavelengths or wavelength ranges and a generated output for the other or another one of the at least ...

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Номер записи: 92
11-06-2020 дата публикации

LIQUID FILTRATION SYSTEM WITH INTEGRATED BLEED FUNCTION

Номер: US20200181554A1
Автор: GRANT Paul
Принадлежит: The Automation Partnership (Cambridge) Limited

A liquid filtration system according to the present invention comprises a bioreactor; a filter configured to filter liquid passing therethrough; a perfusion pump configured to move liquid between the bioreactor and the filter; a liquid line providing fluidic communication between the bioreactor, perfusion pump and filter; and a bleed outlet provided on the liquid line, the bleed outlet configured to provide means to remove liquid selectively from the system under the action of the perfusion pump. With the liquid filtration system according to the present invention, both the filtering and bleed functions may be performed under the action of a single pump, thereby significantly reducing the complexity and cost of the components required. 1. A liquid filtration system for perfusion of a cell culture comprising:a bioreactor for holding a cell culture liquid;a filter configured to filter cell culture liquid passing therethrough;a perfusion pump configured to move cell culture liquid between the bioreactor and the filter;a liquid line providing fluidic communication between the bioreactor, perfusion pump and filter; anda bleed outlet provided on the liquid line, the bleed outlet configured to provide means to remove cell culture liquid selectively from the system under the action of the perfusion pump.2. The liquid filtration system of wherein the bleed outlet is provided by an additional outlet in the perfusion pump.3. The liquid filtration system of wherein the bleed outlet further comprisesa first pinch valve configured to selectively open and close the bleed outlet.4. The liquid filtration system of further comprising a second claim 3 , associated pinch valve claim 3 , the second pinch valve configured to close the liquid line when the first pinch valve is open.5. The liquid filtration system of further comprising one or more sensors configured to determine one or more parameters of a liquid in the system.6. The liquid filtration system of wherein the parameters ...

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Номер записи: 93
11-06-2020 дата публикации

Pulsatile Perfusion Bioreactor for Mimicking, Controlling, and Optimizing Blood Vessel Mechanics

Номер: US20200181556A1
Автор: EBERTH JOHN F., GORE CONRAD MICHAEL, PRIM DAVID A., SHAZLY TAREK, ZHOU BORAN
Принадлежит:

A pulsatile perfusion bioreactor for culturing one or more engineered blood vessels having a lumen and a wall is provided. The bioreactor includes a chamber for holding the engineered blood vessel and cell culture media; a mechanical property monitoring system for measuring axial tensile stress and strain, circumferential tensile stress and strain, and/or shear stress imparted on the vessel wall; and a pump system for delivering cell culture media through the vessel lumen, wherein the vessel is exposed to a composite pressure waveform and a composite flow waveform as the media is delivered there through. The pump system includes a steady flow and peristaltic pumps. Further, the composite pressure and flow waveforms each include a mean component, a fundamental frequency component, and a second harmonic frequency component. The bioreactor also includes a computer interface for monitoring and adjusting the composite waveforms to maintain a predetermined stress level. 1. A method of culturing a one or more engineered blood vessels having a lumen and a wall inside a pulsatile perfusion bioreactor , the method comprising:inserting the engineered blood vessel to be cultured into a chamber;filling the chamber with cell culture media;delivering cell culture media through the lumen of the engineered blood vessel via a pump system, wherein the engineered blood vessel is exposed to a composite pressure waveform and a composite flow waveform as the cell culture media is delivered through the lumen, the pump system comprising a steady flow pump and a peristaltic pump, wherein the composite pressure waveform comprises a mean pressure component, a first harmonic frequency pressure component, and a second harmonic frequency pressure component, and wherein the composite flow waveform component comprises a mean flow component, a first harmonic frequency flow component, and a second harmonic frequency flow component;measuring axial tensile stress, circumferential tensile stress, shear ...

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Номер записи: 94
22-07-2021 дата публикации

ORGANOID CULTURE SYSTEM AND METHOD FOR STERILISING AN ORGANOID CULTURE SYSTEM

Номер: US20210222108A1
Автор: ARCE VAZQUEZ Victor Manuel, COSTOYA PUENTE Jose Antonio, GOLAN CANCELA Irene, PARDO VAZQUEZ Jose Luis, ZUMALAVE RIVAS Jose Antonio
Принадлежит:

The invention relates to a modular sterilizable system for organoid culture, which comprises a culture module with one or more sample wells and an stirring module that includes an air compressor with flow control means, a nozzle for each sample well, a pressure sensor and a controller that acts on the flow control means according to the pressure reading. The method for sterilizing the system consists in decoupling the two modules, removing the culture module and sterilizing it. The system can have a module for monitoring the growth of the organoids, as well as a module for controlling the physiochemical parameters of the culture. 1. A sterilizable organoid culture system comprising:{'b': '2', 'claim-text': {'b': '4', 'one or more sample wells ();'}, 'a culture module () comprising{'b': 8', '4, 'a stirring module () comprising stirring means for each sample well ();'}characterized in that:{'b': '8', 'claim-text': [{'b': '81', 'an air compressor system comprising flow control means () configured to supply a pressurized air flow at a given pressure;'}, {'b': 82', '4', '4, 'a nozzle () for each sample well (), configured to channel the pressurized air flow to each sample well ();'}, {'b': 83', '84', '84', '81', '81', '83, 'a pressure sensor () and a controller (), the controller () being configured to act on the flow control means () by varying a supply power of the flow control means () based on a pressure reading provided by the pressure sensor ().'}], 'the stirring module () comprises28483. A system according to claim 1 , wherein the controller () is configured so that the pressure reading provided by the pressure sensor () corresponds to the pressure transfer/time curve selected for each experiment.38. A system according to wherein the stirring module () comprises:{'b': 5', '51', '52', '4, 'a cover () comprising discharge means (, ) for discharging air from each sample well ().'}48. A system according to wherein the stirring module () comprises:{'b': 81', '82, 'an ...

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Номер записи: 95
22-07-2021 дата публикации

Automated incubator with robotic transport

Номер: US20210222110A1
Автор: Alan Blanchard
Принадлежит: Thrive Bioscience Inc

A cell incubator system comprising a housing having an internal chamber having a controlled temperature and gas mixture therein appropriate for the growth of particular cells for incubation of the particular cells in one or more cell culture vessels in the internal chamber and a plurality of cell operation stations in the internal chamber. The stations receive plates having wells for holding cells and wherein the cell operations stations perform operations on cells in the wells. The system has a robotic transport for moving plates from one station to another.

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Номер записи: 96
23-07-2015 дата публикации

EFFLUENT MONITORING SYSTEMS

Номер: US20150203805A1
Автор: Price Derek
Принадлежит:

We describe a system for monitoring effluent discharge to determine one or both of a biochemical oxygen demand (BOD) and a toxicity of the discharge. The system comprises: a culture vessel comprising a sealable chamber for culturing a fluid sample and a pressure measurement transducer for measuring a pressure in a headspace of the chamber; and a data processing system to: input pressure data from the pressure measurement transducer; and determine a value for one or both of BOD and toxicity from the change in pressure measured by the pressure transducer. 1. A system for monitoring effluent discharge to determine one or both of a biochemical oxygen demand (BOD) and a toxicity of said discharge , the system comprising:a culture vessel comprising a sealable chamber for culturing a fluid sample and a pressure measurement transducer for measuring a pressure in a headspace of said sealable chamber; anda data processing system to:input pressure data from said pressure measurement transducer; anddetermine a value for one or both of said BOD and said toxicity from said change in pressure measured by said pressure transducer.2. A system as claimed in comprising a pair of said culture vessels claim 1 , wherein one said culture vessel is a control.3. A system as claimed in wherein said culture vessel is removable for disposal.4. A system as claimed in in combination with bacteria for said culture vessel claim 1 , wherein one or both of said culture vessel and said bacteria are provided in a sealed enclosure.5. A system as claimed in in combination with bacteria for said culture vessel claim 4 , wherein two sets of said bacteria are provided in respective sealed enclosures claim 4 , one for a control culture claim 4 , and wherein said sealed enclosures are physically linked together.6. A system as claimed in in combination with bacteria for said culture vessel claim 1 , wherein said bacteria comprise bacteria of at least two different types claim 1 , characteristic of different ...

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Номер записи: 97
13-07-2017 дата публикации

LIQUID FEEDING DEVICE AND CELL CULTURE DEVICE

Номер: US20170198249A1
Автор: IGARASHI Yumiko, KIYAMA Masaharu, Nakajima Ryota, Nakamura Taku, Nozaki Takayuki, SUGAYA Masakazu, Suzuki Daisuke, TAKEDA Shizu, TERADA Koichi, ZHOU Guangbin
Принадлежит:

There is the problem that a liquid culture medium fed by a pump has a high frequency of contact with a gas during movement in a tube, and the pH value of the liquid culture medium before and after feeding changes easily. In a liquid feeder and a cell culture apparatus provided therewith, a feed pipe is connected with a gas bag the liquid to be fed into the feed pipe is fed so as to be sandwiched by a gas for suppressing the qualitative changes of the liquid. Furthermore, the gas bag is connected with a container for holding a liquid, and holds the gas for suppressing qualitative changes of the liquid in a gas phase of the container. 1. A liquid feeder comprising:a receptor connected to one end of a feed pipe for feeding a liquid, for receiving the liquid;a gasbag connected to the other end of the feed pipe, for holding a gas for suppressing qualitative changes of the liquid; anda liquid feeding mechanism part for feeding by sandwiching the liquid to be fed to the receptor by the feed pipe.2. The liquid feeder according to comprising:a liquid bottle holding the liquid,flow channels connecting the feed pipe to the liquid bottle; andbranch points branching and connected to the flow channels connected to the gas bag,wherein the liquid feeding mechanism part includes a pump placed in the feed pipe, and a gas introduction valve for introducing the gas of the gas bag to the branch point.3. The liquid feeder according to claim 2 ,wherein when the branch point is provided above the liquid level of the liquid held in the liquid bottle, andwhen the liquid in flow channels connected to the liquid bottle returns to the liquid bottle by dropping, the flow channels are filled with the gas.4. The liquid feeder according to claim 3 ,wherein the liquid feeding mechanism part further includes:an air pressure adjustment pipe for connecting the liquid bottle to the gas bag; anda gas continuity valve for controlling the opening and the closing of the air pressure adjustment pipe.5. The ...

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Номер записи: 98
18-06-2020 дата публикации

METHOD AND APPARATUS FOR LINING A CHAMBER

Номер: US20200188868A1
Автор: CARITE Christophe, Zambaux Jean-Pascal
Принадлежит:

The present invention provides a method and kit which can be used to prevent or reduce contaminants in a clean production environment. The method involves providing a processing apparatus having a chamber, and providing a liner within the chamber, wherein a negative pressure is applied to the interior of the chamber to retain the liner against the interior wall of the chamber. 121.-. (canceled)22. A method comprising:(a) providing a chamber with a flexible liner displaced therein, wherein the chamber comprises a wall with an air permeable opening, wherein the flexible liner comprises an outer surface and an inner surface, wherein the flexible liner has a thickness of from 0.1 μm to 500 μm, and wherein the flexible liner has an air permeable opening;(b) applying a negative pressure through the air permeable opening of the chamber and maintaining the negative pressure within the chamber for a period of time, thereby retaining the flexible liner against the wall of the chamber; and(c) applying a positive pressure to an interior of the flexible liner via the air permeable opening in the flexible liner to inflate the flexible liner while applying the negative pressure into the interior of the chamber.23. The method of claim 22 , wherein the flexible liner has an oxygen transmission rate of less than 5000 cm·m·d.24. The method of claim 22 , wherein the flexible liner has a water vapor transmission rate of less than 200 g·m·d.25. The method of claim 22 , wherein the flexible liner is of a material selected from the group consisting of: polyvinylchloride claim 22 , latex claim 22 , polyethylene claim 22 , polypropylene claim 22 , polyetheretherketone claim 22 , polyester claim 22 , nylon claim 22 , polytetrafluoroethylene claim 22 , silicone rubber claim 22 , cellulose claim 22 , polylactic acid claim 22 , and perfluoroalkoxy alkane.26. The method of claim 22 , wherein the flexible liner further comprises an access opening.27. The method of claim 26 , further comprising ...

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Номер записи: 99
30-07-2015 дата публикации

BIOREACTOR EXHAUST

Номер: US20150210974A1
Автор: Niazi Sarfaraz K.
Принадлежит: Therapeutic Proteins International, LLC

An exhaust system suitable for high volume exhaust from flexible disposable bags is described that prevents nutrient media volume loss and prevents cross-contamination without using any filters. The invention described here allows the use of disposable two-dimensional bioreactors for the cultivation of bacterial and other organisms and cells require high aeration. 1. A method of ventilating a bioreactor comprising:(a) providing a bioreactor exhaust assembly;(b) winding and seating completely the exhaust tube in the groove in the vertical cylindrical block;(c) attaching the top end of the exhaust tube to the condenser block;(d) cooling down the cylindrical block to a temperature between 5° C. and 8° C. by turning on and recirculating the cooling liquid in the cylindrical block;(e) attaching the lower end of the exhaust tube to a gas outlet in the bioreactor;(f) letting the exhaust from the bioreactor enter the exhaust tube;(g) letting the condensate form in the exhaust tube;(h) letting the condensate in the exhaust tube flow back in the bioreactor;(i) measuring the pressure inside the exhaust tube using the pressure sensor attached to the condenser box;(j) releasing the exhaust gas to atmosphere by opening the low pressure relief valve when the pressure in the exhaust tube reaches a pressure above 0.05-inch (±10%) water gauge;(k) closing the low pressure relief valve and stopping the exhaust of gas to atmosphere when the pressure inside the condenser box reaches below 0.05-inch (±10%) water gauge; and(l) repeating steps (i)-(k) till the end of the bioreaction cycle.2. A method of ventilating a bioreactor comprising:(a) providing a bioreactor exhaust assembly;(b) winding and seating the exhaust tube in the groove in the vertical cylindrical block;(c) attaching the top end of the exhaust tube to the condenser block;(d) cooling down the cylindrical block to a temperature between 5° C. and 8° C. by turning on and recirculating the cooling liquid in the cylindrical block ...

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Номер записи: 100