Homogenous thermal shift ligand binding assay
Номер патента: EP3183580A1
Опубликовано: 28-06-2017
Автор(ы): Daniel K. Treiber, Elena MENICHELLI
Принадлежит: DiscoveRx Corp
Опубликовано: 28-06-2017
Автор(ы): Daniel K. Treiber, Elena MENICHELLI
Принадлежит: DiscoveRx Corp
Реферат: Disclosed are methods for detecting and quantitatively measuring a binding property of a compound to a target macromolecule, wherein the target macromolecule is subject to denaturation and is linked to a labeling peptide, such as a short enzyme fragment. The method uses a fluid mixture comprising (i) a chimeric molecule comprising a target macromolecule linked to the labeling peptide and (ii) a compound being measured for binding to the target macromolecule, wherein said target macromolecule is subject to denaturation. After allowing for binding of the compound (e.g. a small molecule inhibitor of the target macromolecule), one detects a signal from the labeling peptide, such as by enzyme fragment complementation. This signal indicates a differential between denatured and non- denatured target macromolecules and thereby indicates a differential between target macromolecules not bound to the compound and target macromolecules bound to the compound, respectively.
Homogenous thermal shift ligand binding assay
Номер патента: US09618516B2. Автор: Daniel K. Treiber,Elena MENICHELLI. Владелец: DiscoveRx Corp. Дата публикации: 2017-04-11.