Method for displaying the sound fruit or vegetable carbon dioxide a freshly cut climacteric.

[0001] the present invention relates to a method for displaying the sound fruit or vegetable carbon dioxide a freshly cut intelligent climacteric, which storage area for fresh foodstuff belongs.

[0002] fresh cut fruit and vegetables are also as a cut fruit and vegetables, semi-processed fruit and vegetables and processed fruit and vegetables are characterized by fresh or slightly known, direct human consumption and well nutrients obtained from. A climacteric fruit or vegetable fleshy sound fruit respiration rate during the time period between dramatic growth experienced in its growth position and initiation of the aging process, wherein the amount of ethylene formed inside the fruits (climacteric fruit maturation-promoting hormone undergoes during or before a) rapid increase also. This is often connected with the freshness of the fruits and the ripening process until a state can be enjoyed. Even after a slight cutting klimakterisches a fruit or vegetable has a menopause, a freshly cut sound fruit or vegetable climacteric why the fresh mass to some related c0 concentrations of₂ and ethene is in its packaging.

[0003] as a new packaging technology, the last decades in the foreign food industry is appeared, the intelligent food packaging technique allows a positive engagement and ensuring the quality and safety of goods by means of distribution for a novel packaging materials, - - structures and forms, consumers selecting safe and reliable food what facilitates, contributes to the storage and transportation of food and good market prospects promises. An intelligent package with indicator function detects the quality, safety and other data of the packaged articles during storage especially through monitoring the change of environment in the package or the property packaged articles, which is of great importance for the security and the nutritional value of foodstuffs. The indicator function of a intelligent package is mostly by indicating a change of data including the temperature, of the gas, the pH, of certain volatile substances and the microorganisms within the package implemented, wherein such change in control by a built-in indicator is displayed in the package.

[0004] a c0₂ - Concentration based application to the packaging a freshly cut fresh indicator is sound fruit or vegetable climacteric potentials. An existing intelligent package with fresh display usually pH color indicator used as reactant, wherein as conventional staining solution for pH sensitive indicator Bromothymol blue labels against change, xylenol blue, bromocresol green, bromocresol purple, phenol red, methyl red, etc. are used. A conventional optical gas-sensitive material also porphyrin-based compounds also raise and applies is usually in the manufacture of gas sensors.

[0005] a Hu yunfeng et (2014) developed as an indicator of change in c0₂ - Concentration within a food package a c0₂ - Mixing indicator, which from a solution of methyl red and blue in a mass ratio of 3:2 and bromothymol is a proportion by weight of 5% and a clear color change in an environment with a c0 each₂ - Volume fraction of 0, 5%, 7%, 13%, 18%, 34%, 43%, 61%, 78% undergoes. On this basis the team of authors was (2014) by means of methyl cellulose (a proportion by weight of 1.8%) and glycerol (a proportion by weight of 4%) as a gelled indicator label for c0 hautsächliche gelling substances₂ - In terms of quantity. In an ink composition for testing carbon dioxide (authorized publication number: is CN 100,340,857 carbon) is the color in a pH indicator abhänigkeit, an adhesive and a solution determined, wherein the pH indicator bromocresol green at least one of the substances, methyl red, chlorinated phenol red, bromocresol purple, bromothymol blue, neutral red, phenol red, cresol red, A naphtholphthalein, curcumin, meta-cresol purple, of ethyl - (2.4 type dinitrophenyl) - diacetate, thymol blue, para-xylenol blue and phenolphthalein contains. A color-changing indicator means (registration number is CN 104,777,162 alpha ., a U is CN 204,575,542) has an indicator portion on, which with carbon dioxide, nitrogen or oxygen and can react with the increase in reaction time has different colors, so as to enable a determination of the fresh product. At a food freshness indicator card for displaying 0 ¾ as gas and a manufacturing method therefor indicative (application number: is CN 104,048,958 α) is used as a base film for a polylactides film indicator card, wherein a composite indicator solution of Bromothymol blue and methyl red deposited onto said base sheet and dried, a complete indicator card. With respect to the present invention is that it is the above-mentioned c0₂ - Mixing indicators synthesized chemicals to low security, a clear color change for a broad region of the change in the c0₂ - Amount need not necessarily sound fruit and vegetable and fresh cut can find application in packaging. Despite the advantages such as the wide color change region and the clear color change in the indicator labels for such conventional reaction substances are possible pollution problems due to insufficient security as regards food pigment used solution of manufacturing process and migration of the pigment solution.

[0006] natural pigment-sensitized substances chemically synthesized substances are far beyond their safety and can thus use in the pigment sensitized intelligent packaging for food find width. In color development component substances it is mostly natural pigment is sensitized to anthocyans, at different pH values or different redox systems also different colors. To Wang et guilian (2014) created an intelligent indicator label for freshness of strawberries, carrots pigment solution is used as a pH sensitizer and the color of the indicator label within the seasoning and maturation phase of strawberries - changed. With respect to the present invention needs this indicator label direct contact with strawberries, wherein despite safety during its extraction and the whole fresh carrots pigment used as indicator label (pH indicator, carrier, transparent base material) are further certain security risks. However the invention used a secure indicator label, natural pigment and does not require direct contact with fresh cut fruit or vegetables, which provides for increased safety.

[0007] in a manufacturing process for a natural pigments based optical food odor sensor (authorized publication number: 102,507,550 b is a CN) are pigments from four raw materials, black rice therefore, flowers of Rhododendron simsii, peach blossom and violet sweet potatoes to different pH values extracted and (4 - 10) conditioned, wherein each thereof under a weakly acidic, neutral and weakly alkaline condition having a clear color change pigment as gas-sensitive agent, fixed and dried on a substrate material, to obtain a matrix of optical sensors. With respect to the present invention is this optical sensor for detection and other meat products used pigmeat odor, wherein the color of the color sensitive material damaged meat products towards alkaline condition changed, fresh cut fruit or vegetables during the course of a fish and meat products undergoes different odor - spoiling process to change, a change in direction so alkaline condition, so that the a color reaction of the triggering thresholds are very different pigment.

[0008] as described so far, it is existing c0₂ - indicator labels usually chemically synthesized substances of low pH color reaction to security. However in the present invention is a natural pigment is used as an indicator, which is extracted by means of ultrasound technology, wherein a two-layer indicator layer comprising a pigment and a gelled film and the film produced natural pigment containing moremore bauwollfaserpapier natural pigment and the cotton fiber paper, a natural pigment film is adhered on the surface thereof, with a layer of sealing are wrapped lPDE film, a c0₂ - complete indicator map, the use in a packaging bag and a packaging box for a fresh cut fruit or vegetable can apply klimakterisches. The menopause or before the sound fruit or vegetable climacteric changes the color of the c0₂ - indicator map toward a acids-related color, namely by the violet for red-violet, a decrease of the sound fruit or vegetable refers to what fresh, sound fruit or vegetable during decay of the fresh cut when the color of the c0₂ - indicator map further toward a acids-related color, red for changed from red-violet so, what indicates, that the fruits or vegetables not enjoying value more.

[0009] of the present invention is based on the, for repair of the preceding disadvantages a method for displaying the sound fruit or vegetable carbon dioxide a freshly cut provide intelligent climacteric, which sound fruit or vegetable fresh a freshly cut an intelligent recognition of climacteric allows, wherein a color change of the indicator label toward a acids-related color on a decrease indicates fresh.

[0010] a method for displaying the sound fruit or vegetable carbon dioxide a freshly cut intelligent climacteric, characterized by the following concrete steps:

[0011] (1) extracting natural pigment: pitting of the fruits of Myrica titles, mixing with water in a mass ratio of 1:1 moremore entsteinter fruits of Myrica titles, form an even suspension obtained, an HCl solution having a concentration of 0.3% to add mass 0.4% as the extractant, twice the volume of the uniform suspension of whose volume corresponds, ultrasonic treatment for 20-to-30 minutes with an ultrasonic power of 360 W is, a pause of 4 s after an operating time of 4 s and a temperature of 60 °c ultrasonic protection, to obtain a liquid crude extract for anthocyans,

Adding a pure ethanol in the crude extract obtained in the volume ratio of 1:1 for liquid anthocyans, by mixing and further sonication for 10 io 15 minutes with an ultrasonic power of 360 W is, a pause of 4 s after an operating time of 4 s and a temperature of 60 °c ultrasonic protection; bearing for 2 H in the dark at room temperature and collecting the clear supernatant, obtain a anthocyans solution,

Static adsorption of the anthocyans solution by means of the AB 8 resin volume mass ratio of 40:1 in, acidified ethanol eluent having a pH of 3 and a collecting the eluate; removing the ethanol and partially water by concentrating the eluate at a vacuum value of 10 kpa and a temperature of 37 °c, to obtain a concentrated anthocyans solution, which solution is referred to as natural pigment and a volume, which volume corresponds to the fifth to sixth before their focus,

[0012] (2) preparing a c0₂ - sensitive indicator solution for natural pigment is:

Conditioning the from step (1) by means of a natural pigment solution obtained with a concentration of 0.2 mol/l NaOH solution to a pH of 5 io 6, dipping a cotton fiber paper in terms of the natural pigment before-conditioned pH solution, removing and drying of the paper for 10 hours at room temperature, a cotton fiber paper having a film adhered to obtain natural pigment, by mixing the terms borrowed the pH conditioned natural pigment with a methylcellulose solution 2.0% to 2.5% and a proportion by weight of glycerol with a volume content of 3.0% to 3.5% a, plates for 24 hours at room temperature and dry cast, natural pigment to form a film; film enveloping the natural pigment and the cotton fiber paper density, with a natural pigment film is adhered on the surface thereof, with a layer of lPDE film, a c0₂ - complete indicator map.

[0013] (3) testing: adhering the from step (2) c0 produced₂ on an inner side of a packaging bag or a packaging box lndikatorkarte - for a fresh cut fruit or vegetable klimakterisches, without direct contact with the fruit or vegetable to come, and determining the freshness of the fresh cut sound fruit or vegetable based on the dramatic increase in c0 by₂ The color change during menopause - concentration caused c0₂ - lndikatorkarte, wherein the range of the color change of the indicator card between 0% and 5% - ^ the co concentration is triggered.

The sound fruit or vegetable climacteric or before the menopause that the color of the c0₂ - lndikatorkarte towards acids-related color, so changed from violet to red-violet, a decrease of the sound fruit or vegetable refers to what fresh, sound fruit or vegetable during decay of the fresh cut when the color of the c0₂ - lndikatorkarte further toward a acids-related color, red for changed from red-violet so, what indicates, that the fruits or vegetables not enjoying value more.

[0014] advantageous effect of the invention: the present invention provides a method for efficient extraction available natural pigment, in ultrasound for extracting water and alcohol used for acidic precipitation and a c0₂ for intelligent recognition of a freshly cut - lndikatorkarte fresh sound fruit or vegetable climacteric is prepared, wherein a color change triggering range of c0₂ 0% and 5% - concentration between and said indicator is a self-produced is natural pigment with a high safety. It is specifically provided, a two-layer indicator layer of cotton fiber paper that, the pigment is adhered on the surface thereof, and a gelled film natural pigment with a layer of good sealing and then vented lPDE film wrapped on the inside of a package are adhered, without direct contact with the fresh cut fruit or vegetables to pass.

The vegetables can be a green leaf vegetables.

[0015] embodiment 1: a method for intelligent displaying the carbon dioxide from the fresh cut (climacteric) broccoli

Pitting of the fruits of Myrica titles, mixing with water in a mass ratio of 1:1 moremore entsteinter fruits of Myrica titles, form an even suspension obtained, adding a HCl solution having a concentration of 0.4% mass as extractant, twice the volume of the uniform suspension of whose volume corresponds, for 30 min with an ultrasonic power of 360 W is ultrasonic treatment, a pause of 4 s after an operating time of 4 s and a temperature of 60 °c ultrasonic protection, to obtain a liquid crude extract for anthocyans,

Adding a pure ethanol in the crude extract obtained in the volume ratio of 1:1 for liquid anthocyans, by mixing for 15 min with an ultrasonic power of ultrasonic treatment and further 360 watts, a pause of 4 s after an operating time of 4 s and a temperature of 60 °c ultrasonic protection; bearing for 2 H in the dark at room temperature and collecting the clear supernatant, obtain a anthocyans solution,

Static adsorption of the anthocyans solution by means of the AB 8 resin volume mass ratio of 40:1 in, acidified ethanol eluent having a pH of 3 and a collecting the eluate; removing the ethanol and partially water by concentrating the eluate at a vacuum value of 10 kpa and a temperature of 37 °c, to obtain a concentrated anthocyans solution, which solution is referred to as natural pigment and a volume, which volume corresponds to the fifth before their focus,

Conditioning the from step (1) by means of a natural pigment solution obtained with a concentration of 0.2 mol/l NaOH solution to a pH of 6, dipping a cotton fiber paper in terms of the natural pigment before-conditioned pH solution, removing and drying of the paper for 10h at room temperature, a cotton fiber paper having a film adhered to obtain natural pigment, by mixing the solution with regard to the pH conditioned natural pigment with a methyl cellulose and a proportion by weight of 2.0% to 3.5% in a volume fraction of glycerol, slab casting and dry at room temperature for 24h, natural pigment to form a film; film enveloping the natural pigment and the cotton fiber paper density, with a natural pigment film is adhered on the surface thereof, with a layer of lPDE film, a c0₂lndikatorkarte - finish.

The c0₂ - within a transparent box for fresh cut broccolilndikatorkarte is adhered, to pass without direct contact therewith. At a violet c0₂lndikatorkarte - may be considered, that the freshly cut broccoli is still fresh, wherein at a red-violet c0₂ it indicates - lndikatorkarte, that the freshly cut the climacteric broccoli highs, the area of the c0₂ 300g fresh broccoli - concentration at a package with cut between 4.0% and 4.5% is introduced and a spoiling process already, while a red c0₂ - lndikatorkarte means, that the freshly cut broccoli is already spoiled.

[0016] embodiment 2: a method for intelligent displaying the carbon dioxide from freshly cut (climacteric) mangoes

Pitting of the fruits of Myrica titles, mixing with water in a mass ratio of 1:1 moremore entsteinter fruits of Myrica titles, form an even suspension obtained, adding a HCl solution having a concentration of 0.3% mass as extractant, twice the volume of the uniform suspension of whose volume corresponds, ultrasonic treatment for 25 min with an ultrasonic power of 360 W is, a pause of 4 s after an operating time of 4 s and a temperature of 60 °c ultrasonic protection, to obtain a liquid crude extract for anthocyans,

Adding a pure ethanol in the crude extract obtained in the volume ratio of 1:1 for liquid anthocyans, by mixing and further ultrasonic treatment for 12 min with an ultrasonic power of 360 W is, a pause of 4 s after an operating time of 4 s and a temperature of 60 °c ultrasonic protection; bearing for 2 H in the dark at room temperature and collecting the clear supernatant, obtain a anthocyans solution,

Static adsorption of the anthocyans solution by means of the AB 8 resin volume mass ratio of 40:1 in, acidified ethanol eluent having a pH of 3 and a collecting the eluate; removing the ethanol and partially water by concentrating the eluate at a vacuum value of 10 kpa and a temperature of 37 °c, to obtain a concentrated anthocyans solution, which solution is referred to as natural pigment and a volume, which volume corresponds to the sixth before their focus,

Conditioning the from step (1) by means of a natural pigment solution obtained with a concentration of 0.2 mol/l NaOH solution to a pH of 5, dipping a cotton fiber paper in terms of the natural pigment before-conditioned pH solution, removing and drying of the paper for 10h at room temperature, a cotton fiber paper having a film adhered to obtain natural pigment, by mixing the solution with regard to the pH conditioned natural pigment with a methyl cellulose and a glycerol 2.5% in a volume fraction of a proportion by weight of 3.25%, plates and dry at room temperature for casting 24h, natural pigment to form a film; film enveloping the natural pigment and the cotton fiber paper density, with a natural pigment film is adhered on the surface thereof, with a layer of lPDE film, a c0₂ - complete indicator map.

The c0₂ - indicator map is within a transparent box for fresh cut mangoes adhered, to pass without direct contact therewith. At a violet c0₂ - indicator map can be assumed, that the freshly cut mangoes is still fresh, wherein at a red-violet c0₂ indicator map - it indicates, that the freshly cut the climacteric mangoes highs, the area of the c0₂ Concentration at a package with fresh cut between 3.0% - 3.5% and a spoiling process and the mango 300g is introduced already, while a red c0₂ - indicator map means, that the freshly cut already mangoes is spoiled.