천연 살선충 조성물 및 살선충 활성을 갖는 은사시나무 추출물의 제조방법

Hereinafter, the present invention more detailed as follows.

As above-mentioned is method of recycling damaged our current (Bursaphelenchus Xylophilus) Generated by the method of recycling damaged bottle pine and fired by shafts is a serious etc..' pine aids' infected once the treatment plant diseases referred to this wool without substantially all pines are of caspases. In the case of shafts which mainly pines in our south fat, fat is primarily fired etc. polyaddition of shafts.

Room number method for plant diseases include, 1) number of the nematode which will live about points tree preventive injection injection, 2) mediated charge room number synthetic insecticidal number for spraying air of microorganisms or antagonism, 3) from a room number and nematode infection mediated conductitivy neck for crushing throat infection, such as the pin is fired and fumigation method. However injection injection sampled high mountain zone are expensive to the sonar nothing rim neck infection room number method for room number is effective method or a blend of due to the tropics. In the case of number 7 about a month a number 5 is mainly a synthetic insecticidal activity mediated insectwithin month stabilizers the case air win one effective method, administration of synthetic insecticidal number shown to distributing areas and in a door number cannot be measured disclosed. By environment-friendly mediated charge room number method of release kasugaensis section, as compared to synthetic insecticidal number yet does not have lugs away from the normalizing effect effect are disclosed. The growth of organic synthesis but excellent sustained use beneficial loyalty reduced due to abuse, such as door number for resistance development and in the axis which is toxicity etc. as well.

The letter or number method is the development of new environment-friendly situations in room are disclosed.

The present invention refers to the step number including silver in a silver tree when an extract of a crude extract by a manufacturing method in a silver wood extract the aforementioned door number when the number public box fitting and then, through various facility for cultivating, edible crops, they are folded to a pine acid damage to plural revegetation nematode can room number.

In particular, pine, like applied to wooden fired for a parasitic nematode may have a method of recycling damaged the nematode which will live activity.

The present invention refers to an extract in step number when a silver tree wood extract 24 through a high pressure liquid coolant when a silver silver bath can be extract number.

When the silver for cultivating said extract to the user facility, edible crops, they are folded to a pine acid damage to plural revegetation nematode the nematode which will live as activity, preferably pine, like the method of recycling damaged applied to wooden fired a parasitic nematode (Bursaphelenchus xylophilus) For the nematode which will live activity may have.

On the other hand, silver fiber of the present invention in wood (meristotheca: PopulusTomentiglandulosa) Sensors mounted thereon is tree (P. Davidiana) silver hundred on amount (P. Alba) Resulting from the natural hybrid between are disclosed. Biological classification (Plantae) (Angiospermae) genome (Dicotyledoneae) plant riverplant orgin to protect polysilicon (Salicaceae) me [mwus[mwus] with pizza plant door neck (Salicales) as a reducing to plants, and in particular of the supply pipe to the sleeve coupling army bark is silver lower angled leaves a beautiful injection planting or induction planting, planting can be used as buffer.

On the other hand, when a silver the present strabismus called the other tree also called tree which, specifically, dB the present strabismus tree wood (P. Davidiana) silver hundred on amount (P. Alba) Resulting from the same tree as when silver meristotheca between artificial hybrid have diameters less than 2000. Thus, the artificial tree of the present invention when a silver the present strabismus wooden hybrid implementation being including general outline.

In residual tree structure, including oratorical talentheartwood department and outwardly from the inside of the active injection, can be composed of hard pine bark.

In other words, tree formation layers can be formed about outside hard pine bark, inside alcohol can be hard mask is formed, the present invention can be used when a silver in silver tree tree continuity, preferably when tree formation layers comprising silver, when at least one of eugenia and alcohol can be further comprises silver.

Applying a silver general tree when said extraction solvent extraction can be, using distilled water as a solvent in the inner wall of a high pressure liquid coolant heat through extraction number can be a silver wood extract.

Preferably said silver in tree 90 - 130 °C, preferably at a temperature of 100 - 125 °C chuck in a number can be a silver tree document data to extract high pressure liquid coolant, also said when, method of recycling damaged (BursaphelenchusXylophilus) In order to obtain the extract for the nematode which will live activity can be excellent.

As such, the reason is that when a silver through extraction number extract of a tree when wooden silver heat bath method of recycling damaged (Bursaphelenchus xylophilus) For obtaining an extract for the nematode which will live activity which, if not extracting heat methanol, alcohol solvent such as silver if method of recycling damaged when obtaining it burns and it comes extract (Bursaphelenchus Xylophilus) Extract for the nematode which will live activity thereof can not obtained.

On the other hand, silver in said wood in an extract, the extraction time is 10 minutes - 100 minutes sampling time, preferably 10 - 40 minutes, extracting the number 1 - 4 times, preferably be a 2 - 3 times.

On the other hand, in the present invention refers to extraction of said alcohol and alcohol extract to yield a silver tree alcohol solvent extract through a step number when an extract silver tree extract can be an extract of high pressure liquid coolant tank when a credit card number.

Specifically, when wooden alcohol solvent alcohol extract first silver extraction obtained substrate. Said alcohol is C₁ - C₄ It can be used any alcohol alcohol selected from the group consisting, preferably can be ethanol.

The meaning of the present invention alcohol solvent including 100% alcohol and alcohol or other solvent to be a mixed with the refrigerant. In this case other as a solvent preferably water, ethyl acetate, chloroform and hexane etc. can be, 50 - 95% by weight water alcohol solvent when washed, preferably 60 - 80% by weight of the total extracted using an aqueous solution having a concentration alcohol which can be very advantageous.

When a silver C tree₁ - C₄ Alcohol solvent in extraction, extraction temperature 18 - 70 °C, preferably 20 - 25 °C extraction time is time 20 - 72, preferably 22 - 26 time, extracting the number 1 - 4 times, preferably be a 2 - 3 times.

Next, in a water-alcohol extract by extracting said actuating a silver tree number high pressure liquid coolant therein.

At this time, the pressure-reducing concentrated and said alcohol extract, a high pressure liquid coolant pressure concentrated alcohol extract can be an extract in a silver wood extract number.

Thus, when wooden alcohol solvent extraction of silver obtained alcohol extract, an extract obtained alcohol extract the nematode which will live activity in wood extract is a silver prepared by the number may have.

Further, when the manufacturing method of the present invention extract said chloroform extract of silver in silver tree (Chloroform: CHCl₃ ) In the slurry tank including a solvate fractions number can be extracted.

This increase in time as steps for further active the nematode which will live extract of silver, said silver fractions THon in wood extract may have more enhanced the nematode which will live activity.

Specifically, the present invention refers to a material that when silver silver tree when an extract and chloroform extract said number tank when a silver tree wood extract (Chloroform: CHCl₃ ) Including a number extracting step when a silver bath through a number extract fractions solvate can be high pressure liquid coolant.

In addition, the present invention refers to when wooden alcohol solvent extraction to yield a silver alcohol extract, an extract heat and said tank when said alcohol extract number when a chloroform extract silver tree extract silver tree (Chloroform: CHCl₃ ) Including a number extracting step when a silver bath through a number extract fractions solvate can be high pressure liquid coolant.

As such, the reason is that when a silver extracted using chloroform solvent extract tree using a solvent chloroform extract to reactive method of recycling damaged wood and wood when extracting silver (Bursaphelenchus xylophilus) Extract for the nematode which will live activity can be obtained's oldest. In other words, in addition to the chloroform solvent (hexane) or ethyl acetate (ethyl acetate) [heyk[heyk] People wood extract using a solvent in extracting silver reactive method of recycling damaged if (BursaphelenchusXylophilus) Extract for the nematode which will live activity thereof can not obtained.

On the other hand, the method of recycling damaged wood extract as described in said aforementioned silver (Bursaphelenchus xylophilus) the nematode which will live may have activity for which, preferably based on expressions 1 to when, after processing 48 may have the nematode ratio which it will live 80 - 100%.

[Mathematical equation 1]

Further, the present invention refers to extract composition as the active ingredient in a silver number including natural the nematode which will liveunder public affairs substrate.

At this time, when the number of the silver in said silver extract extract can be extract prepared by the aforementioned manufacturing method.

Specifically, when the silver in said silver tree extract can be an extract in a silver wood extract, when wooden alcohol solvent extraction of alcohol extract extracted actuating the silver in silver be a wood extract.

Said alcohol is C₁ - C₄ It can be used any alcohol alcohol selected from the group consisting, preferably can be ethanol.

In addition, when silver is silver in wooden alcohol solvent extraction of said extract extracted when actuating the alcohol extract heat because of seeds can be a silver or chloroform extract fraction including wood and, when wooden alcohol comprises dipping a silver, a silver or chloroform extract fraction including human health when wood heat because of seeds can be a disclosed.

Said alcohol is C₁ - C₄ It can be used any alcohol alcohol selected from the group consisting, preferably can be ethanol.

In addition, when a silver composition of the present invention the nematode which will live silver tree in tree continuity can be, and preferably is a silver tree formation layers, at least one of eugenia and alcohol can be further comprises when a silver.

In addition, of the present invention natural the nematode which will live composition method of recycling damaged (Bursaphelenchus Xylophilus) For the nematode which will live may have activity, preferably based on expressions 1 to when, after processing 48 can be visible the nematode ratio which it will live 80 - 100%.

[Mathematical equation 1]

On the other hand, the present invention refers to a natural number including the aforementioned natural the nematode which will liveunder public affairsthe nematode which will live composition and number, said number is method of recycling damaged natural the nematode which will live (BursaphelenchusXylophilus) For the nematode which will live activity may have.

In the embodiment of the present invention through to more specifically describe but, in the embodiment of the present invention has the number is relayed to the range, this method for assisting in the understanding of the present invention will be interpreted.

In the embodiment

Preparation example 1: When a silver tree Pine bark, pine bark including formation layers, including alcohol and alcohol formation number bath

After taking a forest gene distal part of injection when a silver material-making brocadcast FM with forest Institute wood, pine bark, pine bark including formation layers, including alcohol and alcohol was separated into hard.

Preparation example 2: Method of recycling damaged of Separation and identification

Method of recycling damaged wood buck the bay the atmosphere law which will peel separating nematode infection throat using a transparent conductive layer, the type and volume of separated nematode in method of recycling damaged by using method of recycling damaged his separating genetic pure water.

The method of recycling damaged mark separated pure germ nuclear powdery mildew rolled (Botrytis Cineria) With respect to the pure culture growth medium to advance after passaging.

In the embodiment 1

Example 1 disclosed in appropriate size prepared in distilled water to 500 ml of a silver fiber isolated from formation layers without further including injection tree bark 60g, extracting 3 times in the bell 20 121 °C have, been found when the filtered, cream or same number when a silver wood extract was a high pressure liquid coolant.

In the embodiment 2

The same method in the embodiment 1 to a high pressure liquid coolant when a silver wood extract his number.

But, instead of example 1 prepared in example 1 are reproduced using a silver injection including isolated from formation layers prepared pine bark of a tree including alcohol injection when isolated from formation layers as well as silver.

In the embodiment 3

The same method in the embodiment 1 to a high pressure liquid coolant when a silver wood extract his number.

But, instead of example 1 prepared in example 1 are reproduced using a silver injection including isolated from formation layers prepared in tree bark 10g isolated from bark of silver injection was used.

In the embodiment 4

The same method in the embodiment 1 to a high pressure liquid coolant when a silver wood extract his number.

But, instead of example 1 prepared in example 1 are reproduced using a silver injection including isolated from formation layers prepared in isolated from alcohol injection tree bark 50g of silver was used.

Comparison example 1

Example 1 disclosed in appropriate size prepared in 500 ml ethanol injection tree bark 60g including isolated from formation layers of silver to without further application, by extracting 3 times at room temperature in 48 time is 20 °C have, been found when the filtered, pressure concentrated ethanol extract was a silver tree number same when high pressure liquid coolant.

Experiment example 1

Example 2 preparation of sterilizing method of recycling damaged and suspended in distilled water, said method of recycling damaged about 100 mary 0 included in the suspension. 5 ml sterile container by less affected by substrate.

In each extract prepared by the number 0 in the embodiment 1 - 4 and comparison of said container. 5 ml was added to embodiment the nematode which will live activity assays.

Said container while dead method of recycling damaged the nematode which will live active assays can be seen when the identifying, using the opening and closing of every predetermined time 24 while checking whether total 3 times collapsing were measured.

the nematode ratio which it will live (%) determined by means of the expressions is a 1, the result table 1 are described.

[Mathematical equation 1]

Table 1 of example 1 are reproduced using isolated from inflammatory and injection when a silver using a number of pine bark extract in a high pressure liquid coolant including formation layers prepared in example 1 in the embodiment 1 of silver and a silver tree tree including alcohol injection using a wood extract isolated from formation layers in the embodiment 2 has been completed is 90% or more when a silver high pressure liquid coolant which are processed a number 48 snow line insect ratio, is 99% or more were able to confirm that the processing has been completed the nematode ratio which it will livecharge 72.

In addition, when silver of example 1 prepared using a high pressure liquid coolant injection of pine bark extract isolated from tree a number when a silver tree in the embodiment 3, when silver of example 1 prepared using a high pressure liquid coolant injection tree isolated from alcohol extract prepared in the embodiment 4 and a number of example 1 silver in silver tree in tree bark extract isolated from formation layers into ethanol injection including recycling damaged the nematode which will live remedies for comparison example 1 were not confirm activity.

In the embodiment 5

Example 1 disclosed in appropriate size prepared in 500 ml ethanol injection tree bark 60g including isolated from formation layers of silver to without further application, by extracting 3 times at room temperature in 48 time is 20 °C have, been found when the filtered, pressure concentrated ethanol extract was a silver tree number same when high pressure liquid coolant.

Said beverage exhibits high without further 500 ml distilled water, extracting 3 times in the bell 20 121 °C have, been found when the filtered, cream or same number when a silver wood extract was a high pressure liquid coolant.

In the embodiment 6

In the embodiment 5 was a method identical to the number when a silver tree to extract high pressure liquid coolant.

But, instead of example 1 prepared in example 1 are reproduced using a silver injection including isolated from formation layers prepared pine bark of a tree including alcohol injection when isolated from formation layers as well as silver.

In the embodiment 7

In the embodiment 5 was a method identical to the number when a silver tree to extract high pressure liquid coolant.

But, instead of example 1 prepared in example 1 are reproduced using a silver injection including isolated from formation layers prepared in tree bark 10g isolated from bark of silver injection was used.

In the embodiment 8

In the embodiment 5 was a method identical to the number when a silver tree to extract high pressure liquid coolant.

But, instead of example 1 prepared in example 1 are reproduced using a silver injection including isolated from formation layers prepared in isolated from alcohol injection tree bark 50g of silver was used.

Experiment example 2

Example 2 preparation of sterilizing method of recycling damaged and suspended in distilled water, said method of recycling damaged about 100 mary 0 included in the suspension. 5 ml sterile container by less affected by substrate.

In the embodiment 5 - 8 0 extract prepared by the number in each said container. 5 ml was added to embodiment the nematode which will live activity assays.

Said container while dead method of recycling damaged the nematode which will live active assays can be seen when the identifying, using the opening and closing of every predetermined time 24 while checking whether total 3 times collapsing were measured.

the nematode ratio which it will live (%) determined by means of the expressions is a 1, the result table 2 are described.

[Mathematical equation 1]

Table 2 prepared in example 1 isolated from formation layers such as a silver solvent ethanol injection of tree bark including particularly as an extract prepared in example 1 in the embodiment 5 of silver in silver prepared by the number to extract isolated from a tree and tree including alcohol solvent ethanol injection hard particularly as an extract to a silver prepared by the number 48 is 85% or more snow line insect ratio time after processing in the embodiment 6 which are in wood extract, 98% or more the nematode ratio which it will livecharge 72 after the first call request identifying processing time.

In other words, in the embodiment 5 in number in the embodiment 2 in the embodiment 1 and the silver in silver prepared by the number in the embodiment 6 and when wood extract similar to method of recycling damaged chargethe nematode which will live activity in wood extract produced therewith identifying first call request.

In the embodiment 9

9 - 1. When a silver treeHeat Extract number bath

Example 1 disclosed in appropriate size prepared in 500 ml ethanol injection tree bark 60g including isolated from formation layers of silver to without further application, by extracting 3 times at room temperature in 48 time is 20 °C have, been found when the filtered, pressure concentrated ethanol extract was a silver tree number same when high pressure liquid coolant.

Said beverage exhibits high without further 500 ml distilled water, extracting 3 times in the bell 20 121 °C have, been found when the filtered, cream or same number when a silver wood extract was a high pressure liquid coolant.

9 - 2. When a silver treeHeat Extract Fractions Number bath

In the embodiment 9 - 1 in chloroform extract organic solvent and solvent fraction in a silver prepared by the number tree, have same extracting 3 times, when the at least two filtered, cream or a silver wood fractions number was same when high pressure liquid coolant.

In the embodiment 10

In the embodiment 9 the method identical to the number when a silver wood fractions was high pressure liquid coolant.

But, instead of example 1 prepared in example 1 are reproduced using a silver injection including isolated from formation layers prepared pine bark of a tree including alcohol injection when isolated from formation layers as well as silver.

In the embodiment 11

11 - 1. When a silver treeHeat Extract number bath

Example 1 disclosed in appropriate size prepared in 500 ml ethanol injection tree bark 60g including isolated from formation layers of silver to without further application, by extracting 3 times at room temperature in 48 time is 20 °C have, been found when the filtered, pressure concentrated ethanol extract was a silver tree number same when high pressure liquid coolant.

Said beverage exhibits high without further 500 ml distilled water, extracting 3 times in the bell 20 121 °C have, been found when the filtered, cream or same number when a silver wood extract was a high pressure liquid coolant.

11 - 2. When a silver treeHeat Extract Fractions Number bath

In the embodiment 11 - 1 in a water-organic solvent and solvent fraction (Hexane) [heyk[heyk] People tree in a silver prepared by the number, have same extracting 3 times, when the at least two filtered, cream or a silver wood fractions number was same when high pressure liquid coolant.

In the embodiment 12

12 - 1. When a silver treeHeat Extract number bath

Example 1 disclosed in appropriate size prepared in 500 ml ethanol injection tree bark 60g including isolated from formation layers of silver to without further application, by extracting 3 times at room temperature in 48 time is 20 °C have, been found when the filtered, pressure concentrated ethanol extract was a silver tree number same when high pressure liquid coolant.

Said beverage exhibits high without further 500 ml distilled water, extracting 3 times in the bell 20 121 °C have, been found when the filtered, cream or same number when a silver wood extract was a high pressure liquid coolant.

12 - 2. When a silver treeHeat Extract Fractions Number bath

In the embodiment 12 - 1 ethyl acetate extract (Ethyl acetate) in organic solvent and solvent fraction number wood heat when a silver produced therewith, have same extracting 3 times, when the at least two filtered, cream or a silver wood fractions number was same when high pressure liquid coolant.

Experiment example 3

Example 2 preparation of sterilizing method of recycling damaged and suspended in distilled water, said method of recycling damaged about 100 mary 0 included in the suspension. 5 ml sterile container by less affected by substrate.

In the embodiment 9 - 12 0 extract prepared by the number in each said container. 5 ml was added to embodiment the nematode which will live activity assays.

Said container while dead method of recycling damaged the nematode which will live active assays can be seen when the identifying, using the opening and closing of every predetermined time 24 while checking whether total 3 times collapsing were measured.

the nematode ratio which it will live (%) determined by means of the expressions is a 1, the result table 3 are described.

[Mathematical equation 1]

Table 3 in the embodiment 9 such as chloroform fraction of time after processing in the embodiment 10 and 48 for an identifying chargethe nematode ratio which it will live 100% cream, this method of recycling damaged than when silver is in the embodiment 1, 2, 5, 6 wood extract (BursaphelenchusXylophilus) Confirm it for better the nematode which will live active queue.

In addition, in the embodiment 11 and ethyl acetate fraction (Hexane) (Ethyl acetate) in the embodiment 12 for an for an [heyk[heyk] People is method of recycling damaged fraction (BursaphelenchusXylophilus) For the nematode which will live activity were not confirm it.