숙지황 분획물을 유효성분으로 포함하는 타박상 치료용 조성물
The present invention refers to active ingredient composition is useful fractions including bruise composition for treating relates to search, more specifically in the jade it recovers sourly, glomerular endothelial cells and an elongated stem of promoting the expression of efficacy of the composition is the jade it recovers sourly, number -2 heme and heme number -1 fractions including pharmaceutical formulation for treating bruise on active ingredient are disclosed. The pads of the bruise (contusion) under dullness by force (gutta, fall prevention) of the muscles of a size such as soft tissue damage caused edema and hemorrhage under blood diseases as (bruises), herb in [e[e] referred as follows. In the case of knocking and internal bleeding must appear, ligament or of a muscle inflammation, edema is where it is generated sequentially, one end surface of a vehicle trailer is recovered and edema inflammation is reduced but, due to the bleeding is prevented from being left behind [e[e] intramuscular maxims. This [e[e] when old and so as the circulation of blood into a wall, and the wall [e[e] muscle pain remains with the SnO 2 is equal to inflammatory diseases occur due to necrosis of blood. needle about therapy in the treatment of such knocking, circulation therapy by an optical body massaging [e[e] simultaneously preventing inflammation, vascular blood circulation by expanding such as physical therapy is important disclosed. However, the Lotus hereinafter for did not have to continue to practices does not in physical therapy, therapeutic massaging [e[e][e[e] is received from this by only about 80% degree alleviate inflammation pain and inflammation fixed on the blood circulation inside the eliminating effect portion selects a controlled [e[e] performance constant treatment should made in the area. On the other hand, iron (Fe) atoms including porphyrin ring number (heme oxygenase, HO) heme the jade it recovers sourly, (porphyrin ring) is composed of a heme (heme) the oxygen molecules to an enzyme for catalyzing an oxidation reaction involved, a compound such as 1 heme the jade it recovers sourly, number (HO) iron (Fe) and carbon monoxide (biliverdin) rented beer [tin is hemoglobin by decomposing (CO) create other (Noguchi [Compound 1] Heme b + 3O2 + 3 ½ NADPH + 3 ½ H+ →biliberdin + Fe2+ + CO + 3 ½ NADP+ + 3H2 O Such as reaction of the billet beer [tin of said red green yellow bilirubin is transformed into a slowly while via heme is being lost, as a result bruises at high speed ([e[e]) are disclosed. Where is the largest HO in a normal activity of spleen, here the old encapsulated red destroyed substrate. The jade it recovers sourly, number -1 heme is a heme the jade it recovers sourly, (heme oxygenase, HO) number (heme oxygenase-a 1, HO-a 1) heme and present presentation form of the jade it recovers sourly, number -2 (heme oxygenase-a 2, HO-a 2) 2. The jade it recovers sourly, heme said number -1 (heme oxygenase-a 1, HO-a 1) oxidative stress, hypoxic, etc. which is excellent in dispersibility is diffused is induced reaction of heavy metal, in particular by reducing or skin bruises bleeding more rapidly by a stand-alone number hemoglobin numerical inflammation or oxidative stimuli heme when antioxidant protection cells which exerts its effect in other (Maines In this regard HO-a 1 and HO-a 2 to studies, it sells the water [thang LPS in stimulating a macrophage (macrophage) expression of a steroidal anti-inflammatory is a known effect by HO-a 1 if, HO-a 1 between the protective oxidative tissue damage, the enemy [hyel vegetal (erythroblast) level of deficiencies in the spleen in HO-a 1 CD49d weakly oxidative erythropoiesis (oxidative erythropoiesis) made by being melted resistance to that known (Oh In addition, it carries on shoulder but d it comes in HO-a 2 is anticoagulant number by the selectively activated to strong (menadion) that have been reported, such as HO-a 1 carbon monoxide (CO) is generated in the process of nitric oxide synthase (NO synthetase) heme metabolism leading and is intended to function, the stored iron is deficient HO-a 2 unit which toxicity, inflammation in four [phu theory are separated carbon monoxide (CO) if a rented beer [tin with the quinone metabolism by HO-a 2 is a feedback of the indebtedness [nyo tube (Tubuloglomerular feedback) is the reuptake of component number nutritional deficiencies and multi [nyo billion billion nutrient ingredients by number which can exhibit symptoms, increasing survival of vascular endothelial cells in hypoxic (hypoxia) that acetaldehyde (Wang However, HO-a 1 or HO-a 2 conventional material is most chemicals in use as long-term expression of osteoporosis, arteriosclerosis, deterioration due to conditions such as door number possibility carcinogenesis resistance and flow tides. The HO-a 1 and HO-a 2 are also derived from natural materials while adverse bleeding caused by the [e[e] and of promoting the expression of ameliorating or preventing the development of compositions that can be bruise on by executing the desireable disclosed. The present invention the natural from a material composition for treating victims of the bruise on effort perform automatically change, fractions of promoting the expression of HO-a 1 and HO-a 2 composition is by bruise on treatment can be to contain and thus, the present invention has been completed. The purpose of the invention including pharmaceutical composition for treatment fractions the composition active ingredient under public affairs knocking number 30 to 60 seconds. The present invention refers to pharmaceutical composition for treating active ingredient composition is useful fractions including knocking number under public affairs substrate. Used in the present invention the term "bruise treatment" is also under dullness (gutta, fall prevention) or the like pads of soft tissue from damaging force generated [e[e] and massaging of the muscles, caused by prevention and improvement in hemorrhage under blood components to each other. In the present invention composition is used ( In the present invention, said composition is useful fractions THon using hydrothermal extraction composition is then extracted from extracted was filtered, C1 To C4 The alcohol solution obtained seeds, stem and extending in glomerular endothelial cells HO-a 1 and HO-a 2 of activity substance potency relative to said substrate. Said C1 To C4 Lower alcohol solution methanol, ethanol, propanol, butanol, normal - propanol, ISO - propanol, normal - butanol, 1 - rearranged pentanols, using 2 - butoxyethanol or ethylene glycol can be, preferably ethanol, more preferably 50% to 70% ethanol, 60% ethanol than preferably use now. One particular as in the embodiment, of the present invention composition is of a total weight of 10 times water composition is then wet organic material composition is useful fractions THon hyperkeratosis, 95 to 100 °C in 2 to 4 hours then heated filtering, said filtrate 30 to 99. 5% can be obtained by drawing out seeds into ethanol. Said composition is 60% ethanol fraction obtained water-port elongated in glomerular endothelial cells expression of HO-a 1 uses include fraction obtained composition is about 1. 6 increase to 3 times, about 1 expression of HO-a 2. 4 to 3. 2 times which produces the appearance of the skin has been increasing. The, of the present invention composition is useful pharmaceutical compositions for treating bruise on fractions THon can be. The entire composition of the present invention composition may be a predetermined composition is 0. 00001 to 30% by weight, preferably 0. 0001 to 10% by weight can be said. Said composition is 0 weight fraction. 00001% by weight less than HO-a 1 and HO-a 2 expression promoting efficacy is weak and, exceeds 30% by weight of HO-a 1 and HO-a 2 hereinafter if composition is useful fraction compared to increase efficacy of shape and number of promoting the expression of the waste thus ensuring the safety of the pin is not door number point. According to one aspect of the present invention one embodiment pharmaceutical composition in addition to the recombinant active ingredient composition is useful fractions can be pharmaceutically acceptable. Said pharmaceutically acceptable vehicle used typically in number number provided, carbohydrate current compound (example: lactose, amylose, dextrose, sucrose, sorbitol, mannitol, starch, cellulose, or the like), acacia rubber, calcium phosphate, alginate, gelatin, calcium silicate, microcrystalline cellulose, polyvinylpyrrolidone, cellulose, water, syrup, salt solution, alcohol, gum arabic, vegetable oil (example: corn oil, heat treatment seed oil, soy, olive oil, coconut oil), polyethylene glycol, methyl cellulose, methyl hydroxy benzoate, propyl hydroxy benzoate, talc, magnesium and mineral oils and the like acid including but, limited to are not correct. In addition to the components of the present invention pharmaceutical compositions unexpectedly give rise said lubricating number, wet number, number sweetener, flavor number, number emulsion, suspension number, number further comprises preserving such as can be. A cosmetically acceptable suitable carrier and is about number number number Remington's Pharmaceutical Sciences (19th ed. , 1995) disclosed detailed. Of the present invention can be administered by a variety of routes such as oral or parenteral pharmaceutical compositions unexpectedly give rise and, all of the schemas of unexpected administration can be, for example, oral, rectal or intravenous, muscle, can be administered to the patient by subcutaneous injection. The, transdermal administration is preferably include parenteral route, most preferably by application among all (topical application) is administered locally. Suitable dosage is number number of pharmaceutical composition of the present invention method, administration scheme, patient's age, weight, -, pathological conditions, food, administration time, routes, such as sensitive and reaction speed is determined by the various prescribed for a printed wiring board can be. Therapeutic compositions of the present invention reference adult oral dosage is about number 0. 001 - 100 mg/kg (body weight) in the range. In addition external reference number is 1 when adult. 0 to 3. 0 ml 1 1 in an amount of 1 to 5 times by applying times the now continue for one month or more. Only, or limit the scope of the present invention said dosage is not disclosed. In the embodiment of the present invention invention is hereinafter for person with skill in the art is provided to the pharmaceutical compositions unexpectedly give rise can be according to the method, a cosmetically acceptable carrier and/or excipient about number number number number number tank under anger or otherwise prepared by using unit dose form to ingrowth container number bath 1308. amount. An acid number according to each method of the present invention conventional pharmaceutical compositions unexpectedly give rise, granules number, positive number, number capsule, suspension, emulsion, syrup, aerosol such as oral number type, can be used as external number. Number number there is a guaranteed number of normally used, a specific number, number coupled, wet number, number disintegrating, dilution number or excipients such as surfactants using tank number number number with each other. Positive number number number is solid for oral administration, ring number, acid number, granules number, number or the like included in a capsule, for oral suspension include liquid number number number, number content, oil number, which number syrup like a simple dilution number corresponding commonly used in water, in addition to the paraffin droplet number various excipients, for example wet number, sweetener number, direction number, preservation can be like number. For parental administration number number number for non-aqueous is external, suspension number include propylene glycol (propylene glycol), polyethylene glycol, vegetable oil such as olive oil is used as the alkali can be. On the other hand, the effects of the present invention composition is useful fractions THon fractions from composition is also provided to human toxicity and side effects that are less safe and using said long-term can be a pharmaceutical composition such as can be applied safely. Active ingredient used in the present invention composition is useful in glomerular endothelial cells and an elongated uses include fraction distilling exhibit increasing activity of HO-a 1 and HO-a 2, bruise on are useful as pharmaceutical compositions for the treatment of can. Figure 1 (A) standard article (5 a-HMF), composition is extract (B), (C) Fr provided 1 (water fractions), (D) Fr provided 2 (30% EtOH fractions), (E) Fr provided 3 (60% EtOH fractions), (F) Fr provided 4 (99. 5% EtOH fractions) receives the chromatography indicating graph of HPLC are disclosed. Figure 2 quinone dye composition is useful fraction (fraction water) Fr provided 1, Fr provided 2 (30% EtOH fractions), Fr provided 3 (60% EtOH fractions), Fr provided 4 (99. 5% EtOH fractions) on human hepatocyte (HH) has been treated by a 5 a-HMF concentration on human kidney glomerular endothelial cells in centrifuged (HRGEC) HO-a 1 graph are disclosed. Figure 3 shows a composition is also manufactured by fraction Fr provided 3 (60% EtOH fractions) and/or the [hey it pushed (hemin) glomerular endothelial cells in human hepatocyte (HH) a location on a human kidney (HRGEC) HO-a 1 active measuring graph are disclosed. Figure 4 quinone dye composition is useful fraction (fraction water) Fr provided 1, Fr provided 2 (30% EtOH fractions), Fr provided 3 (60% EtOH fractions), Fr provided 4 (99. 5% EtOH fractions) on human hepatocyte (HH) has been treated by a 5 a-HMF concentration on human kidney glomerular endothelial cells centrifuged in graph (HRGEC) HO-a 2 are disclosed. Figure 5 shows a composition is also manufactured by fraction Fr provided 3 (60% EtOH fractions) and/or the [hey it pushed (hemin) glomerular endothelial cells in human hepatocyte (HH) a location on a human kidney (HRGEC) HO-a 2 active measuring graph are disclosed. Hereinafter, in the embodiment example of the present invention to aid in understanding like detailed the on-sensors other. However, the present invention according to in the embodiment are various other shape can be, in the embodiment of the present invention are interpreted to range limited to don't substrate. With the present invention mean knowledge of the present invention in the embodiment are the art to entire surface account for which ball number are disclosed. In the embodiment 1. Sample number bath and component analysis 1 - 1. Extract or composition is useful composition is useful fraction number bath Extracting 10L and water extract composition is 1 kg the composition when putting into a little, then extracting 3 to 95 provided 100 °C same time filtering, high pressure liquid coolant is dried to his number. 1 kg extract composition is useful fractions THon 10L and water composition is useful when putting into a little, then extracting 3 to 95 provided 100 °C same time filtering, said filtering extract is a water column filled 500g (Fr provided 1) into 100 ml HP 20 3L, 3L (Fr provided 2) 30% ethanol, 60% ethanol 3L (Fr provided 3) or 99. 3L-port number was 5% ethanol (Fr provided 4) the high pressure liquid coolant. The, obtained according to each solvent fraction rate 54. 7% (Fr provided 1), 18, 8% (Fr provided 2), 12. 5% (Fr provided 3), 14. This was 1% (Fr-a 4). 1 - 2. Composition is useful fraction component analysis High pressure liquid coolant composition is useful in a number said 1 - 1 extract, the quinone dye composition is useful fraction Fr provided 1 (water fractions), Fr provided 2 (30% ethanol fractions), Fr provided 3 (60% ethanol fractions) or Fr provided 4 (99. 5% ethanol fractions) then methanol 10 ml each fractions after melting the sword misfortune cream was used. In addition, 5 a-HMF(5 a-Hydroxymethyl-a 2 provided furaldehyde) introducing to methanol 10 ml to 1 to 3 was used. 10 uL 5 μm size particles in liquid chromatograph (liquid chromatograph) by introducing said sword misfortune and then it will be burnt after HPLC (high performance liquid chromatography) filled with silica gel (octadecylsilica gel) for preparation of 5 a-HMF component to him. Also shown to result 1. A hollow cylindrical member in Figure 1, each reactive 5 provided HMF content contained in quantifying the results, fractions is manufactured by Fr-a 1 0. 2 mg/g, 30% ethanol fraction for an Fr-a 2 is 1. 1 mg/g, 60% ethanol fraction 56 is manufactured by Fr provided 3. 5 mg/g, 99. 5% ethanol fraction 5 is manufactured by Fr provided 4. 0 mg/g of 5 a-HMF confirm it is incorporated in the first call. In the embodiment 2. Cell experiments 2 - 1. Experiment material preparation High pressure liquid coolant composition is useful in a number said in the embodiment 1 - 1 water fractions (Fr provided 1), 30% ethanol fractions (Fr provided 2), 60% ethanol fractions (Fr provided 3) or 99. 5% ethanol fractions (Fr provided 4) 1 mg/ml ethanol along each such that melting is made, to the same dilution medium 100, 10, 1, 0. Render 1 ug/ml have been produced. Then, the same applying to a final concentration of 0 to 10 times dilution cell misfortune exposure respectively. 01, 0. 1, 1. 0, 10. 0 ug/ml was prepared number so that the high pressure liquid coolant. 2 - 2. Cell culture The outer surface (ScienCell Research Laboratories, USA) 5% (fetal bovine serum) is small [thay oh serum experiment rig HH human hepatocytes, hepatocyte growth factor (hepatocyte growth supplement; HGS, Cat. No. 5252) and Streptococcus feed solution/1% penicillin-resistant Streptococcus pneumoniae (penicillin/streptomycin solution; P/S, Cat. No. 0503) (Hepatocyte medium; ScienCell Research Laboratories, USA) at a temperature of 37 °C to hepatocyte medium containing 500 ml, 5% CO2 Humidification of latched by using the work through. In addition, a human kidney glomerular endothelial cells (ScienCell Research Laboratories, USA) 5% (fetal bovine serum) is small [thay HRGEC oh serum and endothelial cells growth factor (endothelial growth supplement; ECGS, Cat. No. 1052) and 1% penicillin/streptomycin solution (P/S, Cat. No. 0503) (endothelial Cell Medium; ScienCell Research Laboratories, USA) to 500 ml of medium containing endothelial cells 37 °C, 5% CO2 Humidification of latched by using the work through. 2 - 3. HO-a 1 and HO-a 2 assay Said 2 - 2 human kidney glomerular endothelial cells seeded onto 12 - well plate in human hepatocyte and prepare the HH HRGEC (1 × 106 Cell/well) and, 5% CO2 , In him as time 37 °C 16. Culturing said endothelial cells in said 2 - 1 and madecassoside first experiments prepare the water composition is useful fractions (Fr provided 1), 30% ethanol fractions (Fr provided 2), 60% ethanol fractions (Fr provided 3), 99. 5% ethanol fractions (Fr provided 4) or 5 a-HMF 0 respectively. 01, 0. 1, 1, 10 ug/ml concentration next to him as time 24. In addition, a second experiment is the [hey it pushed the stem cells and endothelial cells (hemin) two divided into group 1 uM concentration without processing anything remaining group is a treating time 2 after culturing, two group 0. 1, 1, 10 ug/ml concentration of 60% ethanol fractions (Fr provided 3) 24 and each processing time him as. Said 2 - 2 human kidney glomerular endothelial cells in human hepatocyte and prepare it regulated HRGEC HH was used. First, said sialidase HO-a 1 first, and collected from each of a culture of a second experiment, HO-a 1 assay kit (Heme oxygenase-a 1 ELISA kit, MyBioSource, USA) using activity of HO-a 1 were measured. Said sialidase HO-a 2 first, and collected from each of a culture of a second experiment, HO-a 2 assay kit (Heme oxygenase-a 2 ELISA kit, MyBioSource, USA) using activity of HO-a 2 were measured. 1 and 2 to 5 also shown to result table. Table 2 to a hollow cylindrical member 1 and also in Figure 5, water composition is 60% ethanol fraction composition is (HH) glomerular endothelial cells processing madecassoside fractions (Fr provided 3) other fractions (HRGEC) and expansion (HH) and expansion (HRGEC) glomerular endothelial cells compared to processing madecassoside cells expressing many HO-a 1 and HO-a 2 was too soon, 60% ethanol fractions (Fr provided 3) concentration when it has processed according to a concentration formed by increased expression of HO-a 1 and HO-a 2 depending on concentration, in particular, the [hey it pushed (hemin) (HH) and extending in glomerular endothelial cells processing madecassoside (HRGEC) HO-a 1 and HO-a 2 expression dose were not inserted is further increased. While, water (Fr provided 1) composition is useful fractions, fraction composition is 30% (Fr provided 2) or composition is 99. (HH) and extending 5% fractions (Fr provided 4) glomerular endothelial cells (HRGEC) processing madecassoside finish composition is useful fractions is similar when the regulated expressing HO-a 1 and HO-a 2, and H0 provided 2 expression dose does not increase the concentration increased when H0 provided 1 were confirm it. The present invention refers to compositions for treating relates to active ingredient composition is useful fractions including knocking, said composition is useful in glomerular endothelial cells and an elongated uses include fraction distilling exhibit increasing activity of HO-a 1 and HO-a 2, bruise on are useful as pharmaceutical compositions for the treatment of can. Then filtering the extract composition is heat hyperkeratosis, C1 To C4 Lower alcohol solution solvent seeds obtained fractions including pharmaceutical compositions for the treatment of active ingredient composition is characterized by knocking. According to Claim 1, said C1 To C4 50% alcohol solution (v/v) to 70% (v/v) ethanol solution characterized by a pharmaceutical composition for the treatment of knocking. According to Claim 1, said number -1 fractions (heme oxygenase provided 1, HO-a 1) and the ham jade it is sour or composition is a heme the jade it recovers sourly, number -2 (heme oxygenase-a 2, HO-a 2) promotes protein expression of pharmaceutical compositions for the treatment of bruise characterized. Concentration (ug/ml) HO-a 1 HO-a 2 HH HRGEC HH HRGEC Controls 52. 66 ± 7. 85 66. 55 ± 2. 23 49. 20 ± 2. 89 54. 30 ± 1. 84 5 a-HMF(0. 01) 98. 05 ± 8. 72 100. 43 ± 2. 89 69. 06 ± 11. 85 75. 66 ± 5. 65 5 a-HMF(0. 1) 108. 32 ± 9. 50 113. 67 ± 13. 02 84. 73 ± 8. 39 87. 82 ± 7. 51 5 a-HMF(1. 0) 124. 96 ± 7. 85 125. 10 ± 16. 96 107. 93 ± 6. 68 102. 90 ± 0. 62 (10) 5 provided HMF 141. 54 ± 6. 98 151. 72 ± 18. 46 122. 54 ± 0. 46 134. 36 ± 0. 01 Fr provided 3 (0. 01) 99. 47 ± 1. 07 105. 99 ± 0. 24 70. 97 ± 1. 95 93. 48 ± 7. 93 Fr provided 3 (0. 1) 133. 11 ± 3. 27 122. 20 ± 1. 10 85. 44 ± 0. 30 111. 16 ± 3. 18 Fr provided 3 (1. 0) 142. 87 ± 7. 31 147. 42 ± 2. 16 104. 59 ± 6. 88 142. 94 ± 1. 13 (10) Fr provided 3 180. 94 ± 5. 43 175. 77 ± 8. 66 142. 67 ± 7. 78 164. 93 ± 0. 11
