SILK NANO-FIBER NEUROGRAM PIPE AND A MANUFACTURING METHOD THEREOF, CAPABLE OF EXCHANGING BODY FLUID THROUGH AN AIR GAP
The present invention refers to fiber spinning silk from silk fiber time of a steam heater and then dope electric radiation by diameter about 200 to 400 nanometer in silk nanofibers are entangled state, the external multi-formed silk nanofiber nerve conduit and relates to manufacturing method thereof. Neural tissue damage development of a traffic means disappeared video information according to a traffic accident, by contaminations environment cancer end portion for generating the same associated and neuronal occurring by adverse side-effects of surgical of wet liquid to flow down (B. Schlosshauer et al. , "Rat Schwann cells inbioresorbable nerve guides to promote and accelerate axonal regeneration," Brain Res. , 963, 321-326, 2003). Neural tissue damage the damaged nervous the organicmolecules, or mobile terminal capacity of muscle paralysis of and sense of a user in a working capacity is with. Such pathology thus cut off may be for treating and neuroscience to a method for directly connecting nerves, relatively low and stored in the databases or use frequency neural normal body portion to remove the damage to the element between the such as being predominantly in a method, there have been used. Double and neuroscience to depth in the dermis at which nerve therapy for directly connecting nerves mix itself with the foam damage neural ultra short length so as to control an operation of, actual is a problem having a predetermined length for sensing a neuronal injury the. and a fixed. Furthermore, to remove the body part during the normal neural tissue when implanted the site of injury (autograft), nerve tissue provides another control part as well as planes causing nerve injury, the site of injury neural tissue form and the size of a nerve tissue implanted in the match in order to form a gradually. These two method using actual clinician result, 25% 5 years after treatment the gastro-intestinal motility dysfunction, 1-3% managed to get the sense and degree been recovery only by exposing and developing the photoresist far enough onto the end report is very low. a results (A. C. Lee et al. , "Controlled release of nerve growth factor enhances sciatic nerve regeneration," Exp. Neurol. , 184, 295-303, 2003). Recently and such of treatment of damaged as replacement of the original direct growth nervous and neuroscience to recovery function crosslinked to serving as a nerve conduit is provided to the protein complex from a studies are supplied only with the proceeds by means of wet liquid to flow down. Nerve conduit acids to epoxygenated fatty acids therein the ends of a nerve cut made artificial tan to tube fitted therein has the depth in the dermis at which nerve connection is numeral key tube inducing a receiver, and the receiver opens method has advantages over. First, which interfere with neural regeneration from surrounding structures scar tissue (scar tissue) can be film substrate includes a concave portion, second, correct axonal in a direction (of central functions components of a neurotransmitter) may thus derive combined configuration information which growth of, third, neural on one side of the side of the case, an secretion promoting material while are within the tube the outer a disrupting the playback can be is and its shielded from. Such nerve conduit of materials for the greatly human tissue (intravenous, neural enveloped, cells such as muscle tissue has been removed), natural polymer (collagen, such as chitosan) and synthetic polymer (silicon, poly [...] (polylactic acid, PLA), polyglycolic acid (polyglycolic acid, PGA), poly [...] -glycolic acid copolymer (polylactic acid-co-glycolic acid, PLGA), polycaprolactone (polycaprolactone), poly [...] -polycaprolactone copolymer (poly lactic acid-co-caprolactone), poly [...] -hydroxy [...] copolymer (poly hydroxybutyric acid-co-hydroxyvaleric acid), poly (poly phosphoester) such as [...]) is studied of wet liquid to flow down. However by using human tissue, which does not sufficient supply of tissue, (autograft) implant self ID when the user client receiving the immune response which is unrestricted use by, natural polymer is (absorption) at room temperature in the case of control the pertinent pixel is generated by the function to nerve conduit is arranged the unmounted store before the card is to be solved by the it is also known. Supply of material, the easily and, synthetic polymer excellent in various properties and reduced in the primary studies using of the objects' traces to. Nerve conduit the following requirements a user assigns is is mounted on the inside. First, nerve tissue reproduced, to be recovered and must remain inner space during which, second, even motion procedure site neural distal section can be maintained is stable, and the appropriate must sheet which has a tensile strength in a flexible and, third, the playback of neural tissue healing biodegrades deserve equal is not required in the control procedures then removed, time, this does not reaction tissue rejection or minimized the new device must be powered on, active energy of the reactive, virus or infectivity of non factor and should, an associate, nerve conduit inward and outward can be exchanged of bodily fluid has permeability to which should have both appropriate, copyright 2001, nerve conduit decomposition product of toxicity is. not have to. Actual clinically non-degradable-back step of at although the tubes are used, after neural reproduction, and the body of a lead remaining at the chronic inflammatory, and regenerating calcium of silicon in neural the pain are gradually is implemented using the same, are reduced and the. In vivo, the lower-melting-biodegradable polymer low plate structure of the nerve conduit row is assigned to an is applied to clinical (American Neurotube Neuroregen yarn product). Suture a plating member, a deposition member used a material (polyglycolic acid, PGA) purifying fluoroethanes jet stream by using a carrier gas, the sensory nerve lacking of less than 3 centimeter for repair of DNS bent one by one by storing in the a drug is in the form of internal even pleating an outer clad pipe was reinforced by structure. On the other hand, in Japanese, 'han' could more long dural defects the purpose used on the same bio-degradable polymer as a material, the noise which passes through and tissue friendly as it does not contain chromium solution collagen outside fill the collagen sponges period heating the solutions which have been applied types of nerve conduit row is assigned to an, animal experiment 8 centimeter results embodiment lacking unharmful repairing is made a perfectly site peripheral nerves as well as the nerves been been found is applied to clinical from years 2002 (25th which registration patent number 10-0718073 call). However, during nerve conduit a current commercial collagen based nerve conduit of which the biodegradation royal tomb or excellent, extracted from animal collagen to fabrication process and the storage are inserted and installed is suitable for mass production are elongated, a diffusion prevention film comprising a clinical expensive thus making will both high gas output and use as a raw, dissolution rate is too rapid and distorted in the thickness, immune response which capable of inducing and infectious diseases, tension effected using the very in vivo is problem. Furthermore, poly [...] (polylactic acid, PLA), poly [...] -glycolic acid copolymer (glycolic acid-co-polylactic acid, PLGA) biocompatible such as synthetic polymer a is verified the nerve conduit based polymer without a void type, and a tube and the tensile strength stability and structure but excellent, and to reduce the original cost of located in a readily exchange of a body fluid, biodegradable period long and more than 1000years 2 because the material acidic decomposition product generated by inflammatory response protruded to the outside of the package. Inventor is in the pre-existing synthetic polymer nerve conduit may be nerve conduit to exploit bottom and right and left of both study the, silk nanofiber nerve conduit this biometric as well as is excellent in, that a body fluid between the conduit through the voids inward and outward and can be exchanged, a flexible and suitable tensile strength and tear has, gastro-intestinal motility dysfunction depth in the dermis at which nerve damage superior recovery function and specificity nerve regeneration therapy effect according to the value of the synthetic polymer nerve conduit in lieu of the damage nerves may be use in treating phases are in equilibrium the present invention, is completed. The present purpose of the invention the silk fiber time of a steam heater and then dope fiber spinning silk from electric radiation by diameter about 200 to 400 nanometer in silk nanofibers are entangled state, the external multi-formed providing a conduit for neural nanofiber silk 410 is pulled out from the engagement. It is another object of the present invention said silk nanofiber nerve conduit of is provided to manufacturing method. Said end of the, silk fiber the present invention refers to time of a steam heater and then dope fiber spinning silk from electric radiation by diameter about 200 to 400 nanometer in silk nanofibers are entangled state, the external multi-formed provides nerve conduit nanofiber silk. Furthermore, the present invention refers to fiber spinning dope preparing a step (step 1); said step 1 to a prepared fiber spinning dope (polyethyleneoxide, PEO) polyethylene oxide coated with electrospun collecting unit by channel shaped step of manufacturing a nanofiber silk (step 2); and said step 2 made in channel shaped silk nano-fiber manufactured by collection unit includes separating an RFID circuit from a (step 3) of nerve conduit nanofiber silk including provides manufacturing method. The present invention according to silk nanofiber nerve conduit are biocompatible as well as excellent, the conduit through the voids between inward and outward and that a body fluid can be exchanged, a flexible and suitable since in similar to an in having tensile strength and tear, help playback of neural damage is moved and specificity superior recovery function nerve regeneration therapy effect according to the value of the synthetic polymer nerve conduit in lieu of the treatment of neural damage can be highly available. The present invention refers to fiber spinning silk from silk fiber time of a steam heater and then dope electric radiation by diameter about 200 to 400 nanometer in silk nanofibers are entangled state, the external multi-formed provides nerve conduit nanofiber silk. Hereinafter, the present invention described a detail the. A silk fiber said growing eating the reel drum out cocoons thread the fiber high tensile strength to speak, of brightening agent for unique, excellent dyeability or the like came to be used in fibrous material higher long. In addition two-stranded fibroin is silk fiber is folded enveloped sericin which a structure that the, fibroin vivo even some organization of the surroundings in friendly as it does not contain chromium predetermined part since the powder, gel aqueous solution such as various prepared food, pharmaceuticals as wetting agents can be. Said silk nanofiber nerve conduit of conduit wall the thickness of the it is preferred that a 0.3 millimeter to 0.1 millimeter. Less than said range when easily by external pressure in internal conduit and causing various problems eccentric, said bending softness occurs outputs signals corresponding to a predetermined condition. Silk nanofiber nerve conduit the cross-section of said fine the fibres, in which has a structure and overlaps boronic acids layer layer , diameter of about 200 to 400 nanometer in silk nanofiber an irregular entangled state, the external multi-formed is preferably not less structure. The conduit through the voids between said inward and outward have the effect of exchanging the of body fluid in an of inward and outward pipe void-free between of body fluid in an synthetic polymer shouldering can have the effect of exchanging the 100ms nerve conduit based can be. damp condition the nerve conduit nanofiber silk said surgical suture cut to a sufficient for connecting to nerves a flexible, tensile strength and tear preferably indicative that. Furthermore, the present invention refers to said silk nanofiber nerve conduit of provides manufacturing method. The present invention according to the nerve conduit nanofiber silk Fiber spinning dope preparing a step (step 1); Said step to a prepared fiber spinning dope 1 (polyethyleneoxide, PEO) polyethylene oxide coated with electrospun collecting unit by channel shaped step of manufacturing a nanofiber silk (step 2); and Said step 2 made in channel shaped silk nano-fiber manufactured by collection unit (step 3) includes separating an RFID circuit from plate is deformed due to heat contraction can be produced by manufacturing method. Hereinafter, the present invention according to said manufacturing method a lever is combined to a body a described more specifically. First, a fiber spinning liquid, which is wet spun 1 the present invention according to said step is step is loaded into a reactor. In silk fiber spinning liquid, which is wet spun fiber said removing sericin (step a); effective for silk fiber removed myself sericin, drying and the electrodialysis of a preparation of the silk fibroin aqueous solution (step b); said silk fibroin aqueous solution by freeze-drying step of manufacturing a silk fibroin sponge (step c); and said silk fibroin sponge a predetermined dissolved in the nonaqueous solvent (step d) filtering the a can prepare the through. Said sericin is fibroin stranded two silk fiber is folded membrane which a structure that the, fibroin said of the surroundings in friendly as it does not contain chromium vivo even some organization since the predetermined part powder, gel aqueous solution such as various prepared food, pharmaceuticals as wetting agents can be. The silk fiber is when the preparing a spinning liquid, which is wet spun silk fiber is to eliminate the sericin in a biocompatible preferably to maximize the. A silk fiber oleic acid sodium, sodium carbonate solution is heated by using the step is performed of immersing a sericin can be removed from the. Silk fiber removed myself sericin said distillate repeated using the washed and dried to be a, calcium chloride/water/ethanol mixed solution of particles having a diameter of, dialysis film distilled water introduced to the electrodialysis of a silk fibroin aqueous solution can be produced. Is 12 to 14 kDa MWCO dialysis in said dialysis layer it is preferable that the layer on the integrated circuit substrate through. Silk fibroin aqueous solution said silk fibroin by freeze-drying time of a steam heater and then a sponge, / H2O of 1 kg/0.5L a filtering the spinning liquid, which is wet spun to step is performed can be produced. Said spinning liquid, which is wet spun spinning liquid, which is wet spun rayon fiber concentration of 18% to 12 set the scene for electrospinning process, smooth performance, uniform in the form of fibers preferably to. 12 to 18% in concentration range when the size of a fiber increases the concentration is increased. Concentration of a raw fluid which is to fiber spinning said less than said range when content of silk in spinning liquid, which is wet spun out the fiber is too low, the said viscosity of solution too high a fiber forming a capillary tube inserted stably is difficult unable to proceed electric spinning manner so that the ultra a causing various problems. Next, the said step 1 step 2 to a prepared fiber spinning dope (polyethyleneoxide, PEO) polyethylene oxide coated with electrospun collecting unit by channel shaped step of manufacturing a nanofiber silk is. Said polyethylene oxide (polyethyleneoxide, PEO) a collection unit coated with 5 to 15% polyethylene with oxide coated metal rod is preferably not less. Said range less than the entire surface of metal rod when uniformly causing various problems and is placed, said polyethylene oxide layer is thickened by the refrigerant cooling means is too a causing various problems. Said step 1 to a prepared fiber spinning dope the hermetic container such as a syringe containing the constant volume delivery pumps using the channel shaped at a controlled rate can be nano-fibre production silk. The syringe needle end of the contact tip are made of metal material, such as it is preferable that the used. Said electric radiation power supply device, electrode, spinneret, ground, spinning liquid, which is wet spun delivery unit, consisting of collecting electric radiation market can be by using. Said power supply device a upon radiation, a triarylaminosilane, a tetraarylaminosilane and applying a voltage DC power as delivered to the air from the base a voltage range it is preferred that a 20 kV to 5. Said device and a power supply electrode is connected from connected to said spinneret (injection needle) the metallic material conduits in the venting cap, and signal transmitted from the video signal input emitting portion can be float in electrode. A ground said spinning liquid, which is wet spun high voltage delivery pumps a metered dose responsible delivery outlet for closing of rainwater is made in two portions ground of and collecting manholl open.. Said collection unit a channel type to produce circular metal rod of coat titanium oxide film may be used in, a desired site nerve conduit inner diameter of diameter of and guided along a, use can be made of, metal rod. Said metal rod collection unit during an electric radiation uniformly nano-fiber manufactured by surface in order to apply a co-rotating at a speed of 300 rpm to 100 it is preferable that the. Next, the 3 said step said step 2 made in channel shaped silk nano-fiber manufactured by collection unit is separated from.. Said step 2 after performing a electrostatic spinning of said collection unit meth anol or ethanol to the insoluble nano-fiber manufactured by silk an objective article is immersed of 7:1.. Said process the silk nanofiber a disposable the scene of labor nerve conduit. difficult to use. After, an objective article is immersed water collection unit said silk nano-fiber manufactured by said. may be separated from the collection unit. Separated silk nanofiber nerve conduit consists of distilled water of flushed by additional the storing can be made. Furthermore, the present invention refers to 5 to 15% polyethylene with oxide coated metal rod collecting unit silk nano fiber spinning dope after electric scraps, anol or ethanol to the collection unit an objective article is immersed the insoluble nano-fiber manufactured by silk, 50W %, 0.5-20W % polyethylene an objective article is immersed water by dissolving silk nano-fiber manufactured by said collection unit separated from the same provides nerve conduit nanofiber silk. Hereinafter, and in the embodiment of the present invention. as further described and/or at least two different, stage, of the following embodiment examples of the present invention relate a is, contents of the following embodiment of the present invention and/or at least two different limited not. < in the embodiment1aodirenc> silknanofibernerve conduitfor manufacturing Step 1: materials of fiber spinning Oleic acid from silkworm short and thin whiskers dried sodium carbonate (sodium carbonate) 40 g of sodium (sodium oleate) are each 0.3, immersed 1L 0.2%(w/v) dissolved in an aqueous solution, followed by heating the 1 time and repetitive offset boil sumps remove the sericin the washed. High dimensional removed myself sericin again dry calcium chloride/water/ethanol is 1/8/2 to a solution mixed at a molar ratio from 1:4, at rates of 1/20 was dissolved in 85 °C 3 minutes. Dissolved solution dialysis membrane (MWCO: 12-14 kDa) distilled water is enclosed in the electrodialysis of a 3 to 2%(w/w) and out during about concentration representing a silk fibroin aqueous solution is obtained. Said silk fibroin aqueous solution by freeze-drying silk fibroin suitable dissolution to combat the difference 2 sponge-, (purity 98% or more) to/H2O of 1 kg/0.5L same concentration of 13%(w/v) in completely dissolving the fiber spinning dope have been prepared. 2 step: manufacturing of nanofibers silk channel shaped Collection unit to 1.65 millimeter diameter coated with oxide polyethylene 10% using metal rod of said step 1 a fiber spinning dope produced in 10 kV of the applying voltage having a, the rate of release of the/time 0.2 milliliter, 200 rpm of metal rod rotational speed on the electric spinning manner so that the ultra performed for all the. Silk nanofibers are be integrated in a collection unit thereafter 2.1 millimeter to 2.0 after a time when electric spinning manner so that the ultra, the composition is left for to the middle or the lower cover can be volatile WIPO drying been produced with at nano-fiber manufactured by silk channel shaped. 3 step: silkchannel shaped separation of nanofibers Said step 2 obtained at the channel shaped silk nanofibers are integrated collection unit is immersed for time 1 to ethanol silk nano-fiber manufactured by insoluble and then, said collection unit is immersed for water separated the from silk nano-fiber manufactured by collection unit. Silk flushed by distilled same nanofiber nerve conduit is obtained. <1aodirenc e.g. experiment > measuring structural features of a ship Silk nanofiber nerve conduit of the present invention according to to economize in structural features of a ship for test data of is performed for all the for. For nerve conduit nanofiber silk said in the embodiment 1 also photograph showed to 1. Furthermore, said in the embodiment 1 silk nanofiber nerve conduit of SEM takes a picture also showed to 2. More specifically the reproductivity of the laser diode by nerve conduit nanofiber silk been shown in of Figure 2 (a), of gravity of the robot in its cross-section of Figure 2 (b) and (c) said been shown in, fibrous structure of silk nanofiber nerve conduit surface showed to of Figure 2 (d). Also 1 as demonstrated, the present invention according to in the embodiment 1 silk nanofiber nerve conduit has a circular cross section, structure and empty inner portion and a property of, conduit wall has a thickness of from about 0.2 millimeter can be viewed in. Of Figure 2 (a), (b), (c) and (d) as demonstrated, the present invention according to in the embodiment 1 silk nanofiber nerve conduit the cross-section of fine the fibres, in which has a structure and overlaps boronic acids layer layer , diameter of about 200 to 400 in silk nanofiber an irregular entangled it is found that the structure. The present invention according to in the embodiment 1 through a fine nerve conduit nanofiber silk an irregular fibers integral entangled state, the it is found that the. Thus the nerve conduit nanofiber silk the present invention according to the conduit through the voids between said inward and outward the existing have the effect of exchanging the of body fluid in an pipe void-free cannot be have the effect of exchanging the of body fluid in an inward and outward between a synthetic polymer based nerve conduit can be 100ms. <2aodirenc e.g. experiment > measuring mechanical properties Wettability on nerve conduit nanofiber silk the present invention according to to see for for test data of is performed for all the. Nerve conduit of tensile characteristics in order to test length 4 centimeter conduit was produced. Nerve conduit the inner diameter of 1.65 millimeter cd1a., average the thickness of the duct wall was m micro 200. The area of a cross-section thus 1.16 millimeter2 ([{ (1.65+0.4) /2}2-(1.65/2)2] ×π) was. Conduit followed by immersion for time 1 as saline to the embodiment for tensile test set forth. Amount of sample stuffs to saline are fixed in tester end. Min/10 millimeter to 2 centimeter the Gauge length is made at speeds in the order of steel cutting tensile until determined, it was determined that elongation at. Table 1 showed to result to the computer of the a. believer cutting with said table 1 is about 250%, an elongation at yield point and and out about 20%, ultimate tensile strength is 4.92 MPa to in that it exhibits -65 sufficient tear suture can be viewed. The present invention according to in the embodiment 1 through a surgical nerve conduit nanofiber silk suture cut to a sufficient for connecting to nerves it is found that the. Thus the present invention according to a surgical nerve conduit nanofiber silk suture, use can be made of, to. <3aodirenc e.g. experiment > nerve regeneration for measuring effectiveness of The present invention according to silk nanofiber nerve conduit of to see an effect on nerve regeneration for for test data of is performed for all the. Randomly mouse ischial extracts 8 millimeter in the middle of the femoral nerves to cut a wafer adhered with a length with a fixing member of the back after removing the control group a voice't do something, 8 millimeter in the middle of the ischial nerves to cut a wafer adhered with a length to self neural after removing the, again by replacing the behind the control group positive implantation of the self, 8 millimeter in the middle of the length, and cut into a ischial nerves after removing the 10 millimeter for nerve conduit nanofiber silk the present invention according to in the embodiment 1 neural length the implant to the site of injury is divided into predetermined size sub group the experiments. Main 1 to post-surgical such as said, main 4, main 7, 10 is moved in and out in function main interval each test joint to be measured (ankle stance angel test, ASA) was for enforcing strongly. RAT put track working are in and the underlying material surface is next scene is calculated for one frame to a user an Image to be human power by operating all systems by through analysis program. To measure recovery sense in addition the sole of a foot pressure test (paw pressure test, PP) also performed for all the. The transparent plastic hemisphere has the hole the decrease eye on a murine is the sole of a foot pressure measuring device of the pressure tip of RAT under the pressure increasing gradually after putting a foot the rats have painful shouted when it was determined that pressure of the moment. The, ice due to fear an experiment using a computer to an individual reaction (freezing reaction) was excluded from the show. Result showed to table a 2 and 3. <3-1>Each test joint Articulation angle (ASA) in intermediate steps of walking represents the angle-oriented and telescoping knee normal of RAT 90° value of when the. Result showed to a matrix table 2. <3-2>Sole pressure test Sole pressure (PP) rats are sore event high pressure time (g) exhibits from dripping sense resulted in smaller dimensions. Result showed to a matrix table 2. Table 2 and 3 as demonstrated, articulation angle (ASA) 90° when ever normal treatment with a fixing member of the back an auto voice untreated main 10 after the control group in the case of 42 ° (47% of RAT normal), benign implanting neural user the control group in the case of 57 ° (63% of RAT normal) but a function of, the present invention according to in the embodiment 1 silk nanofiber nerve conduit implanting a mitral valve annuloplasty ring 62 ° (69% of RAT normal) when the is measured by using a the experiment from magnesium, strontium and calcium, been show a motor function improving the best, the voice at a significant a motor function improving material and positive the control group better a motor function improving has been observed on. Furthermore, nerve injury 10 week or two (PP) pressure the sole of the foot measuring untreated treatment nothing to show for his in the case of the control group voice 16.09 g, benign implanting neural user in the case of the control group 25.15 g, silk the present invention according to in the embodiment 1 when the implanting a mitral valve annuloplasty ring nerve conduit nanofibers 15.58 g has been determined. The present invention according to in the embodiment 1 silk nanofiber nerve conduit implanting a mitral valve annuloplasty ring a voice recovery of the sensory nerve, in this case, the cannot determines. The clinical actually of a mammalian motor neuron of a mammalian motor neuron than the cost of large since the recovery of, the present invention according to the nerve conduit nanofiber silk useful in patient nerve injury can be used. <4aodirenc e.g. experiment > self cutting index measuring Peripheral nerve injury as a result of the cutting of the which are conditions occurs frequently most neural species by angiogenesis as a result, cooperation with the degree of cutting self submasters thus permitting pain is observed to animals are degree of pain sensation that the. can reflect. Transplantation of nerve conduit nanofiber silk the present invention according to by to see degree of pain of nerve damage for test data of is performed for all the for. A surgical 3 e.g. experiment 3 said one group for cut self is subject to centrifugal separation has been observed on. 1 occurrence of cutting self levels of a nail step self cutting, cutting self levels of toe comprises a 2, 3 comprises a toe and at a pressure ranging above sole levels of self cutting, 3 or more is the sole of the foot he got down the main divided into cutting of or more. Complete step user is cut and recovery respect to the case 1, the projection unit is inserted into the recovery complete or several self step 2 serious septic tank. so, the serving of an ad. The, cooperation with the self-cutting from being lowered due to serious pain reaction was excluded from the an experiment using a computer to an individual. Result showed to a matrix table 3. Said table 3 with a, treatment with a fixing member of the back the control group voice untreated benign implanting neural user and the control group in the case of post-surgical 1 to 2 in or self cutting is started, the present invention according to in the embodiment 1 silk nanofiber nerve conduit implanting a mitral valve annuloplasty ring I've taken is stock price 4 post-surgical when the user is cut and is started up it is found that the. Furthermore, voice the ratio generating cutting for establishing a self-step 2 the control group, positive the control group, or similar both in the embodiment 1, the ratio generating cutting or several self step 3 post-surgical 10 main during. In the case of the control group voice 67%, 33% positive the control group in the case of, in the case of in the embodiment 1 can be viewed in 0%. The present invention according to in the embodiment 1 through silk nanofiber nerve conduit implanting a mitral valve annuloplasty ring or several self step 3 when the 0% ratio generating cutting the nerve injury since the neuropathic pain occurrence rate of positive molecular weight organic solvent, diethyl ether to get significantly reduced even can be. Of the actual clinical is the sensory nerve in patients to chronic pain according to recovery of, thereby preventing the bicyclic complications important of nerve damage a neuropathic pain of. accumulated values in Serial the first power source line is applied. <5aodirenc e.g. experiment > implanted nerve conduit internal measuring The present invention according to silk nanofiber nerve conduit is internal nerve conduit when implanted to see scene of a for test data of is performed for all the for. In the embodiment 1 of 4 e.g. experiment said silk nanofiber nerve conduit of big implanted or not the program has instructed the main 10 after nerve conduit interior the n bit parallel data inputted photographing and second lines are adjacent to each, showed to 3 also same. More specifically silk nanofiber nerve conduit interior been shown in of Figure 3 (a), thus expanding the internal nerve conduit said been shown in of Figure 3 (b), of Figure 3 (c) of Figure 3 (b) in the lateral outputs a signal corresponding to been shown in, of Figure 3 (b) of Figure 3 (d) showed to of gravity of the robot in its by the interlocked movement. As demonstrated of Figure 3 (a), the present invention according to in the embodiment 1 silk nanofiber nerve conduit implanting a mitral valve annuloplasty ring interior nerve conduit when the fully filled is the same city it is found that the. The enlargement of same as demonstrated of Figure 3 (b), nerve conduit filled into the remaining space material fiber yarns are formed of liquid are brought together such as connection with a set of of the bundle of fibres and can be viewed. The enlargement of once again able to same of Figure 3 (c) and (d) as demonstrated, a fine fiber said fiber bundle are brought toward the are formed of liquid which a main body bundle is, urged the noted fibers results in a definite directional with a pin extending an upper side of the trench area can be viewed. Fiber are brought toward the a small bundle into two wavelengths through the optical, small foot the collected and large bundles a while a semiconductor are and fabric an initial distance a the directivity normal neurotransmitter is an important property of tissue. The present invention according to in the embodiment 1 through silk nanofiber nerve conduit implanting a mitral valve annuloplasty ring when the implanted silk nanofiber nerve conduit interior neural axon newly and are differently oriented which are produced and it is found that the. Thus the present invention according to the nerve conduit nanofiber silk when the implanting a mitral valve annuloplasty ring nerve conduit internal to the tissue newly created group in both damage nerves can be use in treating. <6aodirenc e.g. experiment > measuring histological inside nerve conduit implanted The present invention according to process is performed to eliminate the first nerve conduit nanofiber silk ischial to neuronal injury ischial when implanted and cytological of myelination of of nerve fibers to see degree for test data of is performed for all the for. In the embodiment 1 of 4 e.g. experiment said silk nanofiber nerve conduit of big implanted or not the program has instructed the main 10 neural ischial after the memory block paraffin samples behind pak paragraph the fine thickness m micro 10. Mitogen misdiagnosis dyeing (Hematoxylin and Eosin stain, H & E), look brush fast dyeing (Luxol fast blue stain, LFB) for performing the n bit parallel data inputted, obviated and post dyeing the was and filling the discharge, for preservation of long of tissue. [...] microscope photograph of the tissue (BX-51 microscopy, OPUMPUS, Japan) as captured by using the n bit parallel data inputted, Image analysis equipment (KAPPA image analysis, USA) was an analyte using a. For dyeing & E H after undergoing the elimination paraffin tissue, 10 ingredient [...] , 1 minutes misdiagnosis solution dye of 100 big battery is installed in a battery. Brush look tissue for dyeing LFB fast blue solution (0.1% luxol fast blue MSBN and a including acetic acid 0.05% 95% ethanol) in 57 °C unchanged placing into 24 reacted time. Furthermore, alcohol 95% and, after washing the in order a distilled water tank, and water of new nerve cells for dyeing separator for cortical portion 37 °C [...] violet solution (cresyl violet) to 0.1% in 1 he dipped his time. Obviated and post dyeing the tissue dyeing the textured dimensionally stable shelf for the sealed by welding. The, a timing that is specified several seconds layer, a blue dyed to which neural cells is dyeing violet or pink. &E dyeing result been shown in H of Figure 4 (a), LFB dyeing result been shown in of Figure 4 (b), in the embodiment 1 of Figure 4 (b) of Figure 4 (c) result of the enlargement of showed to. As demonstrated of Figure 4 (a), with a fixing member of the back treatment in the case of the control group voice untreated not not comprise radially extending micro 10 since the neural reproducing a thickness m a crust-less slide Image on discontinuous portions and, in locations that consistency of a wide variety of sizes of neural paper created can be viewed. Furthermore, implanting neural user benign the control group in the case of material such that a directed flow in the center thereof a fragment implanted has a central rotary axis extending and no coincidence is notified assembly seems to have rounded out facing peripheral neural species mass is formed on a can be viewed. High cutting result of autoimmune (said table 3) and a neither is clients ask. Furthermore, the present invention according to in the embodiment 1 silk nanofiber nerve conduit implanting a mitral valve annuloplasty ring initially when the conduit into fully into an empty space that was the chunk polarization film and the cytoplasm and nuclear are dyed cells with. a main body. As demonstrated of Figure 4 (b), in the case of the control group and benign the control group voice neural species in be inject fuel by removing conglomeration phenomenon of nerve fibers can be viewed. Furthermore, the present invention according to in the embodiment 1 silk nanofiber nerve conduit implanting a mitral valve annuloplasty ring into nerve conduit when the broader than a few seconds in addition cells blue dyed to a neuronal having a main body a timing that is specified several seconds cells. As demonstrated of Figure 4 (c), in the direction of travel of cells from blue dyed to neural cells assumes a directional side peripheral in the center thereof an upper side of the trench area that are played back it is found that the. The present invention according to in the embodiment 1 through silk nanofiber nerve conduit implanting a mitral valve annuloplasty ring in the center thereof when the neural cells assumes a directional peripheral side also that are played back an upper side of the trench area can be viewed. Thus the present invention according to silk nanofiber nerve conduit the nerve conduit implanting a mitral valve annuloplasty ring when the species the neural nerve tissue inside peripheral side in the center thereof without an upper side of the trench area assumes a directional in both damage reproduced useful in the treatment of a neural, use can be made of,. Therefore the present invention according to silk nanofiber nerve conduit implantation involves the as well as biocompatible contains a, conduit that a body fluid can be exchanged between inward and outward and, a flexible and suitable tensile strength and tear has, normal depth in the dermis at which nerve additional damage (when implantable self) without the recovery of the gastro-intestinal motility dysfunction depth in the dermis at which nerve damage superior according to the value of the nerve regeneration therapy effect synthetic polymer nerve conduit in lieu of the damaged nerves surgically treating can be highly available. Complications important of nerve damage in addition a neuropathic pain generation of the exit unit is:a power expected a. Also the present invention according to Figure 1 shows a matrix gel nanofiber silk electrophotographic and; Also Figure 2 shows a silk nanofiber nerve conduit of the present invention according to and electron micrographs ((a) cross section electrophotographic, (b) cross-section 100 Image is magnified, photo, (c) cross-section 1000 Image is magnified, photo, (d) of a fiber structure, surface neural tubes nanofiber silk); Also the present invention according to Figure 3 shows a silk nanofiber nerve conduit of big implanted or not the program has instructed the main 10 of nerve conduit inside and scanning electron micrographs (internal neural tubes (a), (b) (a) photo to expand a; arrow neural played back and electronic content to be occured display bundle. , (C) (b) of an arrow in neural reproducing represented by electrophotographic the enlargement of laterally bundle, (d) (b) of an arrow in a longitudinal bundle neural reproducing represented by photo in an enlarged size with); Also the present invention according to Figure 4 shows a silk nanofiber nerve conduit of big implanted or not the program has instructed the main 10 electrophotographic analysis histological inside nerve conduit of is ((a) basic dyeing a mitogen misdiagnosis dyeing electrophotographic, (b) neural bundle specifically dyed on the look brush fast blue dyeing (Luxol fast blue stain) electrophotographic, (c) (b) of in the embodiment 1 the enlargement of electrophotographic; within the tube blue filars by bundle is neural is reproduced). PURPOSE: A silk nano-fiber neurogram pipe and a manufacturing method thereof, capable of exchanging body fluid through an air gap are provided to enhance neurogenesis effect and recover exercise and sense. CONSTITUTION: A manufacturing method of a silk nano-fiber neurogram pipe comprises as follows. The silk nano-fiber neurogram pipe is prepared. A silk nano fabric of a neurogram pipe is manufactured by electrospinning the silk nano-fiber neurogram to a collecting unit. The silk nanofiber of the conduit shape is divided from the gathering unit. A collecting unit is coated on polyethyleneoxide. COPYRIGHT KIPO 2011 Silk fiber time of a steam heater and then dope fiber spinning silk from electric radiation by diameter about 200 to 400 nanometer in silk nanofibers are entangled state, the external multi-formed silk nanofiber nerve conduit. Fiber spinning dope preparing a step (step 1); Said step to a prepared fiber spinning dope 1 (polyethyleneoxide, PEO) polyethylene oxide coated with electrospun collecting unit by channel shaped step of manufacturing a nanofiber silk (step 2); and Said step 2 made in channel shaped silk nano-fiber manufactured by collection unit (step 3) includes separating an RFID circuit from semiconductor device using the same comprise the silk nanofiber nerve conduit of manufacturing method. According to Claim 2, said spinning liquid, which is wet spun fiber 1 step Removing sericin in silk fiber (step a); Sericin effective for silk fiber removed myself, drying and the electrodialysis of a preparation of the silk fibroin aqueous solution (step b); Silk fibroin aqueous solution said silk fibroin by freeze-drying step of manufacturing a sponge (step c); and A predetermined sponge silk fibroin said filtering the dissolved in the nonaqueous solvent (step d) includes a step which is prepared to characterized by silk nanofiber nerve conduit of manufacturing method. According to Claim 2, said spinning liquid, which is wet spun which is prepared at a concentration of 12 to 18% to manufacturing method characterized by silk nanofiber conduit. According to Claim 2, said polyethylene 2 step 5 to 15% a collection unit coated with oxide polyethylene with oxide coated metal rod is characterized by silk nanofiber conduit manufacturing method. According to Claim 2, said step 20 kV radiation voltage electrical 2 to 5 characterized by applying voltage range of silk nanofiber conduit manufacturing method. According to Claim 2, the 3 said step said collection unit after performing a electric spinning manner so that the ultra anol or ethanol to the insoluble nano-fiber manufactured by silk an objective article is immersed to thereby manufacturing method characterized by silk nanofiber conduit. According to Claim 7, said silk nano-fiber manufactured by insoluble and then said water collection unit -50W %, 0.5-20W polyethylene an objective article is immersed said nano-fiber manufactured by silk by dissolving purpose: a collection unit characterized by silk nanofiber conduit manufacturing method. 5 to 15% polyethylene with oxide coated metal rod collecting unit silk nano fiber spinning dope after electric scraps, anol or ethanol to the collection unit an objective article is immersed the insoluble nano-fiber manufactured by silk, 50W %, 0.5-20W % polyethylene an objective article is immersed water by dissolving silk nano-fiber manufactured by said collection unit which are produced separated from silk nanofiber nerve conduit. In the embodiment 1 Cutting elongation (%) 243 ±15 Cutting strength (MPa) 4.92 ± 0.07 Yield point (%) 20 ASA (°) PP (g) Voice the control group 1 main 33 60.43 4 main 36 35.79 7 main 38 15.89 10 main 42 16.09 Positive the control group 1 main 37 51.03 4 main 40 23.11 7 main 45 17.00 10 main 57 25.15 In the embodiment 1 1 main 45 45.89 4 main 36 36.85 7 main 45 11.13 10 main 62 15.58 Post-surgical time (main) 1 2 3 4 5 6 7 8 9 10 1 (%) ratio generating step: user is cut and levels of nail Voice the control group 0 20 80 60 60 60 60 60 60 60 Positive the control group 50 50 50 50 50 50 50 75 75 75 In the embodiment 1 0 0 0 17 33 33 50 67 83 83 2 (%) ratio generating step: toe level for establishing a self-cutting Voice the control group 0 0 60 60 60 60 60 60 60 60 Positive the control group 0 25 25 25 50 50 50 50 50 50 In the embodiment 1 0 0 0 0 0 17 33 50 50 50 3 (%) ratio generating step: user is cut and levels of the sole of a foot and at a pressure ranging above toe Voice the control group 0 0 20 40 40 40 40 40 40 40 Positive the control group 0 25 25 25 25 25 25 25 25 25 In the embodiment 1 0 0 0 0 0 0 0 0 0 0 During individual generating cutting self generating step 3 (%) ratio Voice the control group 0 0 25 67 67 67 67 67 67 67 Positive the control group 0 33 33 33 33 33 33 33 33 33 In the embodiment 1 0 0 0 0 0 0 0 0 0 0
