МОДИФИЦИРОВАННЫЙ ФИБРОИН

Verfahren zur Herstellung von Proteinfaser, und Verfahren zum Schrumpfen von Proteinfaser

Die vorliegende Erfindung betrifft ein Verfahren zur Herstellung einer Proteinfaser, umfassend einen Schritt in welchem eine Proteinrohfaser enthaltend ein Protein mit Wasserdampf in Kontakt gebracht wird in einer Lagerkammer, in welcher die Temperatur auf einen Bereich unterhalb von 120°C eingestellt ist, um eine Hitzebehandlung der Proteinrohfaser durchzuführen.

VORRICHTUNG UND VERFAHREN ZUM SPINNEN VON MATERIALIEN

Verwendung von künstlich hergestellter Spinnenseide

Die Verwendung von Saiten (2) an Musikinstrumenten soll den hohen Beanspruchungen, den spezifischen musikalischen Eigenschaften und den guten physikalischen Eigenschaften gerecht werden. Dazu ist künstlich hergestellte Spinnenseide erfindungsgemäß ein Bestandteil der Saite (2).

PROCEDURE FOR THE PRODUCTION OF FIBERS ON THE BASIS OF SILK

DEVICE AND PROCEDURE FOR SPIDERS OF MATERIALS

Fibre-forming process and fibres produced by the process

The present invention relates to a process for the preparation of fibres and fibres prepared by the process. The process can provide discontinuous colloidal polymer fibres in a process that employs a low viscosity dispersion medium.

Metalloprotein compositions

The present invention relates to compositions comprising: a polypeptide, wherein at least a portion of the polypeptide has a coiled coil structure; and a chelate comprising a chelating agent and a metal ion; and wherein the chelate is bound to at least one amino acid of the polypeptide. In a preferred embodiment the polypeptide is a silk fibroin, wherein at least a portion of said silk fibroin has a coiled coil structure.

Composite materials comprising synthetic dragline spider silk

Composites based on a polymer and a mixture of proteins derived from a MaSp (major ampullate spidroin) protein are provides. Further, methods for preparation of same, and method of use of the composites are provided.

Molecularly composite polymeric material of fibroin/cellulose and process for producing the same

BIOPOLYMER OPTICAL WAVEGUIDE AND METHOD OF MANUFACTURING THE SAME

A method of manufacturing a biopolymer optical waveguide includes providing a biopolymer, unwinding the biopolymer progressively to extract individual biopolymer fibers, and putting the unwound fibers under tension. The tensioned fibers are then cast in a different polymer to form a biopolymer optical waveguide that guides light due to the difference in indices of refraction between the biopolymer and the different polymer. The optical fibers may be used in biomedical applications and can be inserted in the body as transmissive media. Printing techniques may be used to manufacture the biopolymer optical waveguides.

SILK-BASED FIBRE

The invention relates to a novel process for preparing a solution of silk or silk-like protein from a variety of potential sources, and spinning it to produce a reformed silk fibre for biomedical and other uses. The silk is pre- dissolved to form an amorphous powder, then re-dissolved in a mixture of dichloroacetic acid with either chloroform or dichloromethane, to form a homogenous silk fibroin solution for spinning. By controlling spinning and drawing conditions, and by appropriate chemical modification, fibres with a variety of mechanical and biological properties can be formed. Such fibres can have an extended or shortened biostability in the human body, and are suitable for implantable devices such as absorbable artificial ligaments and engineering matrices, drug delivery implants, and many other uses.

УСТРОЙСТВО И СПОСОБ ХРАНЕНИЯ ПРОТЕИНОВ

В изобретении предлагается устройство для хранения протеина (20), которое содержит первую камеру (30) для хранения протеина (20) и вторую камеру (40) для хранения щелочного буферного раствора (50), причем вторая камера (40) имеет флюидальное сообщение с первой камерой (30). В соответствии с предпочтительным вариантом осуществления изобретения щелочной буферный раствор содержит ионы кальция, которые способствуют загустеванию протеина (20). Предлагается также способ хранения протеина (20), который включает в себя следующие операции: первую операцию помещения протеина в первую камеру хранения (30); вторую операцию воздействия на протеин (20) при помощи щелочного буферного раствора (50); и третью операцию сохранения протеина (20) в щелочной среде в первой камере хранения (30).

DEVICE AND METHOD FOR STORAGE PROTEINS

Procédé d'obtention de fibres de collagène de peau d'origine humaine, fibres ainsi obtenues et composition les contenant

L'invention concerne un procédé d'obtention de fibres de collagène obtenues à partir de fragments de peau humaine. On traite le matériau de départ pour obtenir, par action d'une solution de chlorure de magnésium, une suspension de fibrilles de collagène; on reforme in vitro les fibres de collagène sous l'action d'un promoteur de fibrillogénèse constitué par un sel d'acide de la glucosamine et/ou l'acide N-acétyl neuraminique; on contrôle en microscopie les fibres obtenues et, si nécessaire, on les lave par une solution de sorbitan; on rince les fibres et on les utilise dans une composition tamponnée pour des injections dermiques sur le sujet qui a fourni les fragments de peau servant de matériau de départ.

POLYPEPTIDE SOLUTION, ARTIFICIAL POLYPEPTIDE FIBER PRODUCTION METHOD USING SAME, AND POLYPEPTIDE REFINING METHOD

This polypeptide solution is a polypeptide solution wherein a polypeptide derived from a natural spider thread protein is dissolved in a solvent, said solvent including at least one selected from formulas (i)-(iii): (i) DMSO; (ii) Inorganic salt added to DMSO; (iii) Inorganic salt added to DMF. In addition, this invention uses the polypeptide solution as a dope liquid, pushes the dope liquid out from a cap into a desolvation tank, separates the solvent from the dope liquid, forms fiber and uses same as undrawn yarn, and obtains an artificial polypeptide fiber. In addition, this invention refines the polypeptide by removing insoluble matter after thermal treatment of the polypeptide solution. As a result, a polypeptide solution, a production method for an artificial polypeptide fiber, and a polypeptide refining method are provided that have high media solubility, are capable of high-temperature melting, are highly safe, and have inexpensive solvent costs.

ELECTROSPUN SILK MATERIAL SYSTEMS FOR WOUND HEALING

The present invention relates to the processes of preparing silkfibroin/polyethylene oxide blended materials, and the resulting materials thereof, which are suitable for biomedical applications such as wound healing. In particular, the electrospun silk fibroin/PEO mats with a silk:PEO blend ratio of 2:1 to 4:1, treated with controlled evaporation, constraint-drying techniques, and/or alcohol treatment, and/or PEO extraction, demonstrate suitable physical and biofunctional properties, such as fiber structure, topography, absorption, water vapor transmission rates, oxygen permeation, and biodegradability, relevant to biomaterial systems with utility for wound dressings.

Method for producing fibroin-like protein

A method for producing a fibroin-like protein is provided. A fibroin-like protein is produced by a method of culturing Escherichia coli having a gene encoding the fibroin-like protein in a medium, inducing expression of the gene encoding the fibroin-like protein, and collecting the fibroin-like protein, wherein accumulation of an organic acid at the time of inducing the expression is reduced, and wherein the gene is expressed under control of a tryptophan promoter.

ELECTROSPUN SILK MATERIAL SYSTEMS FOR WOUND HEALING

Recombinant spider silk proteins

The present invention is directed to recombinant spider silk proteins, nucleic acids, coding for these recombinant spider silk proteins, as well as hosts suitable for expressing those nucleic acids. Furthermore, the present invention is directed to a method of aggregation of spider silk proteins and the use of the proteins in the field of biotechnology and/or medicine and other industrial fields, in particular in the manufacture of automotive parts, in the aircraft construction, in the processing of textiles and leather, as well as in the manufacture and processing of paper and the like.

WATER-ABSORBING AND QUICK-DRYING PROPERTY-IMPARTING AGENT, AND METHOD FOR IMPARTING WATER-ABSORBING AND QUICK-DRYING PROPERTIES

Provided is a water-absorbing and quick-drying property-imparting agent capable of easily imparting water-absorbing and quick-drying properties to various materials or articles in a simple process, and a method capable of easily imparting water-absorbing and quick-drying properties to predetermined materials or articles. A water-absorbing and quick-drying property-imparting agent containing modified fibroin as an active ingredient, and a method for imparting water-absorbing and quick-drying properties to an article, the method including a step of incorporating modified fibroin into the article.

МОДИФИЦИРОВАННЫЙ ФИБРОИН

Изобретение относится к области биотехнологии, конкретно к модифицированному фиброину, включающему последовательность домена, представленную формулой: [мотив (A)n-REP]m. Модифицированный фиброин, в котором по меньшей мере один или множество глициновых остатков в REP заменены другим аминокислотным остатком по сравнению с природным фиброином, сохраняет прочность и растяжимость природного фиброина и может быть использован для получения волокон. 16 н. и 24 з.п. ф-лы, 3 ил., 9 табл., 5 пр.

Applying a liquid protein onto a permeable surface, and silk mono-filament having specific properties

A casting device comprises a permeable protein supporting surface, and a region which allows the protein to be exposed to a fluid. The dried liquid protein is stretched. The preferred protein is artificial, natural or native silk. The fluid is preferably a conditioned gas, having a controlled water vapour. The casting surface comprises at least two layers of water or water vapour permeable material. Preferably at least one of the casting surface layers comprises clay. The silk mono-filament has a breaking force of at least 40 N. The mono-filaments, produced by cutting the membrane, may be used as a musical string or in a medical device.

EQUIPMENT AND PROCEDURE FOR THE PRODUCTION OF SILK FIBERS WITH HIGH FIRMNESS

SILK EMPLOYMENT FORERUNNER FOR THE PRODUCTION OF THREADS

IMPROVED SILK FIBERS

Methods and compositions are provided for improved proteinaceous block copolymer fibers based on long repeat units having molecular weight of greater than about 10 kDal. Each repeat unit includes more than about 150 amino acid residues that are organized into a number of "quasi-repeat units." The fibers have improved mechanical properties that better recapitulate those of the native silk fibers. See Fig. 1. WO 2016/149414 PCT/US2016/022707 Cl)) ct aI) z00 ...

APPARATUS AND METHOD FOR THE SELECTIVE ASSEMBLY OF PROTEIN

The invention describes a method for the selective assembly of proteins into a structure. The method has a first step of the inclusion of a first partial protein sequence -GYG- into a further protein sequence. The protein sequence has in its native state a loop structure. A second step involves the addition of metal ions to thereby form the selective assembly.

METHOD FOR PRODUCING FIBER AND FILM OF SILK AND SILK-LIKE MATERIAL

A method for producing a silk or silk-like fiber, characterized in that it is spun from a solution of silk fibroin and/or a silk-like material in hexafluoroacetone hydrate and optionally is then stretched, or in hat it is casted from a solution of silk fibroin and/or a silk-like material in hexafluoroacetone hydrate followed by drying, and optionally is then stretched.

APPARATUS AND METHOD FOR FORMING MATERIALS

An apparatus and method for forming liquid spinning solution into a solid formed product whereby the solution is passed through at least one tubular passage (17) having walls formed at least partly of semipermeable and/or porous material. The semipermeable and/or porous material allows parameters, such as the concentration of hydrogen ions, water, salts and low molecular weight, of the liquid spinning solution to be altered as the spinning solution passes through the tubular passage(s).

METHOD AND DEVICE FOR PRODUCING A THREAD FROM SILK PROTEINS

The invention relates to a method for producing a thread from silk proteins and to device for carrying out said method. The thus produced threads and th e use thereof are also exposed. The invention uses a high-performance diffusio n unity for producing high-quality silk threads.

A PROSTHETIC FABRIC STRUCTURE

Disclosed herein is an implantable prosthesis for breast augmentation or reconstruction procedures. The prosthesis comprises a biocompatible and biodegradable fabric structure comprising one or more individual yarns comprised of sericin-extracted native fibroin fibers, wherein the yarns are intertwined to produce the fabric structure. The fabric structure extends in at least a first dimension and has at least a first surface that is adapted to engage and support natural breast tissue and/or a prosthetic breast implant in a patient. Also disclosed are methods of supporting breast tissue or a breast implant in a patient by inserting the prosthesis between the skin of the patient and the breast tissue and/or implant within the patient.

Method for preparing conductive regenerated silk fibroin nanofiber membrane

Electrospinning method for mass-preparing nanofibers by novel electrospinning device

PROTEIN FIBER AND METHOD FOR PRODUCING SAME

This protein fiber contains silk fibroin, and the protein fiber has a stress of at least 450 MPa and a rupture elongation of at least 5%. This method for producing a protein fiber is for obtaining the aforementioned protein fiber, obtained by wet-spinning a doped liquid containing silk fibroin, and then performing at least heated stretching under dry heat on the un-stretched thread of protein fiber. The stretched thread or unstretched thread of the protein fiber may be further cross-linked by means of a carbodiimide. As a result, provided are: a protein fiber having high stress and a favorable rupture elongation and a method for producing same.

Concentrated aqueous silk fibroin solution and use thereof

The present invention provides for concentrated aqueous silk fibroin solutions and an all-aqueous mode for preparation of concentrated aqueous fibroin solutions that avoids the use of organic solvents, direct additives, or harsh chemicals. The invention further provides for the use of these solutions in production of materials, e.g., fibers, films, foams, meshes, scaffolds and hydrogels.

Concentrated Aqueous Silk Fibroin Solution and Use Thereof

The present invention provides for concentrated aqueous silk fibroin solutions and an all-aqueous mode for preparation of concentrated aqueous fibroin solutions that avoids the use of organic solvents, direct additives, or harsh chemicals. The invention further provides for the use of these solutions in production of materials, e.g., fibers, films, foams, meshes, scaffolds and hydrogels. 1. An aqueous silk fibroin solution having a fibroin concentration of at least 10 wt % , wherein said solution is free of organic solvents.25-. (canceled)6. The aqueous silk fibroin solution of further comprising a therapeutic agent.7Bombyx mori.. The solution of claim 1 , wherein said fibroin is obtained from a solution containing a dissolved silkworm silk claim 1 , wherein the silkworm silk is obtained from810-. (canceled)11. The solution of claim 1 , wherein said silk protein is obtained from a solution containing a genetically engineered silk1239-. (canceled)40. A method of producing a film comprising the steps of:(a) preparing a silk fibroin solution comprising silk fibroin in an aqueous salt solution;(b) dialyzing said solution against a hygroscopic polymer to result in an aqueous solution of at least 10 wt % of fibroin; and(c) casting the solution of step (b) to form a film.4158-. (canceled)59. A silk fibroin composition having a fibroin concentration of at least 4% silk fibroin but not more than 33% silk fibroin claim 1 , wherein said composition is free of alcohol.60. The silk fibroin composition of claim 59 , wherein the composition comprises between about 10% and about 30% silk fibroin.61. The silk fibroin composition of claim 59 , wherein the composition is an aqueous or solid silk fibroin composition.62. The silk fibroin composition of claim 59 , wherein the composition is a film.63. The silk fibroin composition of claim 59 , wherein the composition comprises multiple layers.64. The silk fibroin composition of claim 59 , further comprising a therapeutic agent.65. The ...

APPARATUS AND METHOD FOR STORING PROTEINS

An apparatus for the storage of a protein (20) comprising a first compartment (30) for storing the protein (20) and a second compartment (40) for storing an alkaline buffer (50), the second compartment (40) being in fluid communication with the first compartment (30) is described. In one preferential embodiment of the invention, the alkaline buffer comprises calcium ions which encourage the gelling of the protein (20). A method for the storage of a protein (20) is also described. The method comprises: a first step of placing the protein in a first storage compartment (30); a second step of exposing the protein (20) to an alkaline buffer (50); and a third step of maintaining the protein (20) in the alkaline atmosphere in the first storage compartment (30).

Method and apparatus for forming objects

Apparatus and method for storing proteins

USE OF ARTIFICIALLY MANUFACTURE SPIDER SILK

A PROCESS FOR SPINNING POLYPEPTIDE FIBERS

A prosthetic fabric structure

Disclosed herein is an implantable prosthesis for breast augmentation or reconstruction procedures. The prosthesis comprises a biocompatible and biodegradable fabric structure comprising one or more individual yarns comprised of sericin-extracted native fibroin fibers, wherein the yarns are intertwined to produce the fabric structure. The fabric structure extends in at least a first dimension and has at least a first surface that is adapted to engage and support natural breast tissue and/or a prosthetic breast implant in a patient. Also disclosed are methods of supporting breast tissue or a breast implant in a patient by inserting the prosthesis between the skin of the patient and the breast tissue and/or implant within the patient.

Highly contracted synthetic fibroin fiber, production method therefor, and method for contracting synthetic fibroin fiber

The present invention relates to a contracted synthetic fibroin fiber comprising modified fibroin, wherein the contraction percentage defined by the following equation exceeds 7%. Contraction percentage={1-(length of contracted synthetic fibroin fiber/length of synthetic fibroin fiber after spinning and before contact with water)}×100 (%) ...

A PROCESS FOR SPINNING POLYPEPTIDE FIBERS

RECOMBINANT SPIDER SILK PROTEINS

PRECURSOR FEEDSTOCK FOR FORMING FILAMENTS

The application relates to a method of obtaining silk feedstock solution from a gland of a silkworm or another silk-producing arthropod in which the body of the silkworm or the other silk-producing arthropod is cut open and the gland removed. The silk feedstock solution is subsequently extracted from the gland and used for extrusion, spinning or moulding of objects.

MODIFIED FIBROIN

The present invention relates to modified fibroin containing a domain sequence represented by formula 1: [((A)n motif)-REP]m or formula 2: [((A)n motif)-REP]m-((A)n motif), wherein the domain sequence has an amino acid sequence having a reduced glutamine residue content in association with the deletion of one or multiple glutamine residues in REP or the substitution of one or multiple glutamine residues in REP by another amino acid residues. [In formula 1 and formula 2, "(A)n motif" represents an amino acid sequence composed of 4 to 27 amino acid residues, wherein the ratio of the number of alanine residues relative to the total number of amino acid residues in "(A)n motif" is 80% or more; "REP" represents an amino acid residue composed of 10 to 200 amino acid residues; m represents an integer of 10 to 300; and multiple pieces of "(A)n motifs" or the like may have the same amino acid sequence as each other or may have different amino acid sequences from each other.] ...

SHAPE MEMORY SILK MATERIALS

The present application relates to silk fibroin-based materials, methods for making and using the same. Provided materials exhibit shape memory characteristics while showing comparable or better volumetric swelling, biocompatibility and/or degradability when compared to current memory polymers derived from either natural or synthetic materials.

COMPOSITE MATERIALS COMPRISING SYNTHETIC DRAGLINE SPIDER SILK

Composites based on a polymer and a mixture of proteins derived from a MaSp (major ampullate spidroin) protein are provides. Further, methods for preparation of same, and method of use of the composites are provided.

ELECTROSPUN SILK MATERIAL SYSTEMS FOR WOUND HEALING

The present invention relates to the processes of preparing silkfibroin/polyethylene oxide blended materials, and the resulting materials thereof, which are suitable for biomedical applications such as wound healing. In particular, the electrospun silk fibroin/PEO mats with a silk:PEO blend ratio of 2:1 to 4:1, treated with controlled evaporation, constraint-drying techniques, and/or alcohol treatment, and/or PEO extraction, demonstrate suitable physical and biofunctional properties, such as fiber structure, topography, absorption, water vapor transmission rates, oxygen permeation, and biodegradability, relevant to biomaterial systems with utility for wound dressings.

SILK BIOMATERIALS AND METHODS OF USE THEREOF

... ²²²The present invention provides an all-aqueous process and composition for ²production of silk biomaterials, e.g., fibers, films, foams and mats. In the ²process, at least one biocompatible polymer, such as poly(ethylene oxide) ²(PEO) (a well-documented biocompatible material), was blended with the silk ²protein prior to processing e.g., electrospinning. We discovered that this ²step avoids problems associated with conformational transitions of fibroin ²during solubilization and reprocessing from aqueous solution which lead to ²embrittled materials. Moreover, the process avoids the use of organic solvents ²that can pose problems when the processed biomaterials are exposed to cells in ²vitro or in vivo.² ...

ELECTROACTIVE BIOPOLYMER OPTICAL AND ELECTRO-OPTICAL DEVICES AND METHOD OF MANUFACTURING THE SAME

A method of manufacturing a biopolymer optical device in-cludes providing a polymer, providing a substrate, casting the polymer on the substrate, and enzymatically polymerizing an organic compound to generate a conducting polymer between the provided polymer and the substrate. The polymer may be a biopolymer such as silk and may be modified using organic compounds such as tyrosines to provide a molecular-level interface between the provided bulk biopolymer of the biopolymer optical device and a substrate or other conducting layer via a tyrosine- enzyme polymerization. The enzy-matically polymerizing may include catalyzing the organic compound with peroxidase enzyme reactions. The result is a carbon-carbon conjugated back-bone that provides polymeric "wires" for use in polymer and biopolymer op-tical devices. An all organic biopolymer electroactive material is thereby pro-vided that provides optical functions and features.

Metalloprotein compositions

SILK NANO-FIBER NEUROGRAM PIPE AND A MANUFACTURING METHOD THEREOF, CAPABLE OF EXCHANGING BODY FLUID THROUGH AN AIR GAP

PURPOSE: A silk nano-fiber neurogram pipe and a manufacturing method thereof, capable of exchanging body fluid through an air gap are provided to enhance neurogenesis effect and recover exercise and sense. CONSTITUTION: A manufacturing method of a silk nano-fiber neurogram pipe comprises as follows. The silk nano-fiber neurogram pipe is prepared. A silk nano fabric of a neurogram pipe is manufactured by electrospinning the silk nano-fiber neurogram to a collecting unit. The silk nanofiber of the conduit shape is divided from the gathering unit. A collecting unit is coated on polyethyleneoxide. COPYRIGHT KIPO 2011 ...

Una fibra grapada a base de fibra natural, procedimiento y aparato para su fabricación.

La solicitud se refiere a un método y aparato para manufacturar fibras cortadas a base de fibra natural. La solicitud además se refiere a las fibras cortadas, lana cruda a base de fibras cortadas y productos comprendiendo tales. Un método comprende proporcionar una suspensión de celulosa incluyendo agua, fibrillas de celulosa refinada y, por lo menos, un modificador de reología, direccionar la suspensión de celulosa a través de una boquilla sobre una superficie, secar la suspensión de celulosa sobre la superficie para formar una fibra y cortar la suspensión de celulosa sobre la superficie para formar fibras cortadas.

MOLDED REGENERATED SILK GEOMETRIES USING TEMPERATURE CONTROL AND MECHANICAL PROCESSING

The present disclosure provides methods for fabricating various regenerated silk geometries using temperature control. In addition to temperature control, mechanical processing can be used to enhance properties of the fabricated article. The present disclosure also provides silk foam and paper-like materials molded using freezer processing.

CONCENTRATED AQUEOUS SILK FIBROIN SOLUTION AND USE THEREOF

The present invention provides for concentrated aqueous silk fibroin solutions and an all-aqueous mode for preparation of concentrated aqueous fibroin solutions that avoids the use of organic solvents, direct additives, or harsh chemicals. The invention further provides for the use of these solutions in production of materials, e.g., fibers, films, foams, meshes, scaffolds and hydrogels.

METHOD FOR MANUFACTURING PROTEIN MOLDED BODY

An object of the present invention is to provide a method for advantageously producing a protein molded article which can solve a problem caused by esterification of a hydroxyl group contained in a protein while maintaining a sufficient strength. A method for producing a protein molded article according to the present invention includes a step of bringing a raw material molded article containing a protein in which a hydroxyl group is esterified into contact with an acidic or basic medium in a state of applying a tensile force, thus hydrolyzing an ester group.

РЕКОМБИНАНТНЫЙ БЕЛОК ШЕЛКА ПАУКОВ, КОДИРУЮЩАЯ ЕГО НУКЛЕИНОВАЯ КИСЛОТА, ВЕКТОР И КЛЕТКА-ХОЗЯИН, ПОДХОДЯЩИЕ ДЛЯ ЕГО ЭКСПРЕССИИ, СПОСОБ ЕГО АГРЕГАЦИИ, СПОСОБ ПОЛУЧЕНИЯ МАТЕРИАЛА ИЗ НЕГО, СОДЕРЖАЩИЙ ЕГО ПРОДУКТ И ПРИМЕНЕНИЕ

Изобретение относится к области биотехнологии, конкретно к получению рекомбинантных белков шелка паука, и может быть использовано в медицине. Рекомбинантным путем получают белки, состоящие из комбинации модулей С, Q и А белков шелка паука. Полученные белки агрегируют и используют для получения различных материалов: сфер, нано- и микрофибрилл, пен, пленок, гелей и покрытий. Изобретение позволяет получить нити из рекомбинантных белков шелка паука, обладающие высокой прочностью на разрыв и эластичностью. 13 н. и 14 з.п. ф-лы, 2 табл., 18 ил. РОССИЙСКАЯ ФЕДЕРАЦИЯ (19) RU (11) 2 415 938 (13) C2 (51) МПК C12N C12N C07K A61L 15/12 15/63 14/435 17/00 (2006.01) (2006.01) (2006.01) (2006.01) ФЕДЕРАЛЬНАЯ СЛУЖБА ПО ИНТЕЛЛЕКТУАЛЬНОЙ СОБСТВЕННОСТИ, ПАТЕНТАМ И ТОВАРНЫМ ЗНАКАМ (12) ОПИСАНИЕ ИЗОБРЕТЕНИЯ К ПАТЕНТУ (72) Автор(ы): ШАЙБЕЛЬ Томас (DE), ХЮММЕРИХ Даниель (DE), АКЕРШОТТ Кристиан (DE) (21)(22) Заявка: 2007102846/10, 21.07.2005 (24) Дата начала отсчета срока действия патента: 21.07.2005 (73) Патентообладатель(и): АЭмЗильк ГмбХ (DE) R U Приоритет(ы): (30) Конвенционный приоритет: 22.07.2004 US 60/590,196 (43) Дата публикации заявки: 27.08.2008 Бюл. № 24 2 4 1 5 9 3 8 2 4 1 5 9 3 8 R U (56) Список документов, цитированных в отчете о поиске: LAZARIS A. et al. Spider silk fibers spun from soluble recombinant silk produced in mammalian cells, Science, 2002, v.295, n.5554, p.472-476. LEWIS R.V. et al. Expression and purification of a spider silk protein: a new strategy for producing repetitive proteins. Protein Expr. Purit, 1996, n.4, p.400-406. SCHELLER J. et al. Production of spider (см. прод.) C 2 C 2 (45) Опубликовано: 10.04.2011 Бюл. № 10 (85) Дата начала рассмотрения заявки PCT на национальной фазе: 22.02.2007 (86) Заявка PCT: EP 2005/007968 (21.07.2005) (87) Публикация заявки РСТ: WO 2006/008163 (26.01.2006) Адрес для переписки: 191036, Санкт-Петербург, а/я 24, "НЕВИНПАТ", пат.пов. А.В.Поликарпову (54) РЕКОМБИНАНТНЫЙ БЕЛОК ШЕЛКА ПАУКОВ, КОДИРУЮЩАЯ ЕГО НУКЛЕИНОВАЯ КИСЛОТА, ...

В ВЫСОКОЙ СТЕПЕНИ СЖАТОЕ СИНТЕТИЧЕСКОЕ ФИБРОИНОВОЕ ВОЛОКНО, СПОСОБ ЕГО ПОЛУЧЕНИЯ И СПОСОБ СЖАТИЯ СИНТЕТИЧЕСКОГО ФИБРОИНОВОГО ВОЛОКНА

FIELD: fiber production.SUBSTANCE: invention relates to artificial fibroin fiber with a high compression ratio. Artificial fibroin web includes modified fibroin, wherein the compression percentage exceeds 7%. Modified fibroin is synthetically obtained fibroin and is a protein comprising a domain sequence represented by the formula: [motif (A)n–REP]m, where motif (A)n is an amino acid sequence, in which the percentage of alanine residues is 40% or more of the total number of amino acid residues, n is an integer of 2-20, REP is an amino acid sequence consisting of 2-200 amino acid residues, m is an integer from 2 to 300.EFFECT: invention provides the production of artificial fibroin fiber, which has a high compression percentage, has excellent tactile properties and elasticity and can be obtained in a safe way.13 cl, 6 dwg, 7 tbl РОССИЙСКАЯ ФЕДЕРАЦИЯ (19) RU (11) (13) 2 757 909 C2 (51) МПК D01F 4/02 (2006.01) C07K 14/435 (2006.01) D06M 11/05 (2006.01) ФЕДЕРАЛЬНАЯ СЛУЖБА ПО ИНТЕЛЛЕКТУАЛЬНОЙ СОБСТВЕННОСТИ (12) ОПИСАНИЕ ИЗОБРЕТЕНИЯ К ПАТЕНТУ (52) СПК D01F 4/02 (2021.02); C07K 14/43518 (2021.02); C07K 14/43586 (2021.02); D06M 11/05 (2021.02) (21)(22) Заявка: 2019130386, 05.03.2018 (24) Дата начала отсчета срока действия патента: Дата регистрации: (73) Патентообладатель(и): СПАЙБЕР ИНК. (JP) 22.10.2021 10.03.2017 JP 2017-046524 (43) Дата публикации заявки: 12.04.2021 Бюл. № 11 (56) Список документов, цитированных в отчете о поиске: US 2005/0010035 A1, 13.01.2005. WO 2012/165476 A1, 06.12.2012. WO 2013/065651 A1, 10.05.2013. RU 2323282 C1, 27.04.2008. (45) Опубликовано: 22.10.2021 Бюл. № 30 (85) Дата начала рассмотрения заявки PCT на национальной фазе: 10.10.2019 2 7 5 7 9 0 9 Приоритет(ы): (30) Конвенционный приоритет: R U 05.03.2018 (72) Автор(ы): СУГАХАРА, Дзунити (JP), МОРИТА, Кейсуке (JP), НАКАМУРА, Хироюки (JP) JP 2018/008199 (05.03.2018) C 2 C 2 (86) Заявка PCT: (87) Публикация заявки PCT: R U 2 7 5 7 9 0 9 WO 2018/164021 (13.09.2018) Адрес для переписки: 129090, ...

РЕКОМБИНАНТНЫЕ БЕЛКИ ШЕЛКА ПАУКОВ

... 1. Рекомбинантный белок шелка паука, содержащий: а) одну или более чем одну синтетическую повторяющуюся последовательность белка шелка паука, где указанная синтетическая повторяющаяся последовательность содержит мультимеризованную (multimerized) повторяющуюся единицу и, возможно, б) одну или более чем одну аутентичную неповторяющуюся последовательность белка шелка паука. 2. Рекомбинантный белок шелка паука по п.1, где последовательности происходят из канатных белков паука из главной ампуловидной железы и/или из белков, происходящих из жгутиковидной железы. 3. Рекомбинантный белок шелка паука по п.1, где аутентичные неповторяющиеся последовательности происходят из аминоконцевого неповторяющегося участка (жгутиковидные белки) и/или из карбоксиконцевого неповторяющегося участка (жгутиковидные и канатные белки) встречающегося в природе белка шелка паука. 4. Рекомбинантный белок шелка паука по п.3, где аутентичная неповторяющаяся последовательность модифицирована для того, чтобы адаптировать ...

PROCEDURE FOR THE PRODUCTION OF SEIDENFIBROINVLIESSTOFFEN

BIOCOMPATIBLE IMPLANT FROM SILK FABRIC

Precursor silk feedstock for forming filaments

RECOMBINANT SPIDER SILK PROTEINS

... ²²²The present invention is directed to recombinant spider silk proteins, nucleic ²acids, coding for these recombinant spider silk proteins, as well as hosts ²suitable for expressing those nucleic acids. Furthermore, the present ²invention is directed to a method of aggregation of spider silk proteins and ²the use of the proteins in the field of biotechnology and/or medicine and ²other industrial fields, in particular in the manufacture of automotive parts, ²in the aircraft construction, in the processing of textiles and leather, as ²well as in the manufacture and processing of paper and the like.² ...

BIOPOLYMER OPTICAL WAVEGUIDE AND METHOD OF MANUFACTURING THE SAME

A method of manufacturing a biopolymer optical waveguide includes providing a biopolymer, unwinding the biopolymer progressively to extract individual biopolymer fibers, and putting the unwound fibers under tension. The tensioned fibers are then cast in a different polymer to form a biopolymer optical waveguide that guides light due to the difference in indices of refraction between the biopolymer and the different polymer. The optical fibers may be used in biomedical applications and can be inserted in the body as transmissive media. Printing techniques may be used to manufacture the biopolymer optical waveguides.

COMPRESSION AND HEAT-ASSISTED PRODUCTION OF SILK-BASED MATERIALS

In some embodiments, the present disclosure provides methods including the steps of (i) providing silk fibroin material comprising substantially amorphous structure, and (ii) applying at least one of elevated temperature and elevated pressure to the silk fibroin material to form a silk fibroin article, wherein the applying induces fusion between at least a portion of the silk fibroin and structural change of fibroin in the silk fibroin material. In some embodiments, the present disclosure also provides silk fibroin articles made in accordance with the methods disclosed herein.

FIBRE-FORMING PROCESS AND FIBRES PRODUCED BY THE PROCESS

The present invention relates to a process for the preparation of fibres and fibres prepared by the process. The process can provide discontinuous colloidal polymer fibres in a process that employs a low viscosity dispersion medium.

DRAWN SILK EGEL FIBERS AND METHODS OF MAKING SAME

The present invention relates to compositions and method for drawing egel silk fibroin fibers. The resulting fibers can transmit light and hence can be used as optical fiber. Silk fibroin fiber is produced by a method comprising applying an electric field to a solubilized silk fibroin solution to create a silk fibroin gel; converting the silk fibroin gel to a viscous silk liquid; and drawing a silk fiber from the viscous silk liquid. The silk fiber of the invention can be used in materials such as textile, medical sutures, and tissue materials, as well as conferring optical properties into these materials.

METHOD AND DEVICE FOR PRODUCING A THREAD FROM SILK PROTEINS

The invention relates to a method for producing a thread from silk proteins and to device for carrying out said method. The thus produced threads and the use thereof are also exposed. The invention uses a high-performance diffusion unity for producing high-quality silk threads.

RECOMBINANT SPIDER SILK PROTEINS

The present invention is directed to recombinant spider silk proteins, nucleic acids, coding for these recombinant spider silk proteins, as well as hosts suitable for expressing those nucleic acids. Furthermore, the present invention is directed to a method of aggregation of spider silk proteins and the use of the proteins in the field of biotechnology and/or medicine and other industrial fields, in particular in the manufacture of automotive parts, in the aircraft construction, in the processing of textiles and leather, as well as in the manufacture and processing of paper and the like.

METHODS AND APPARATUS FOR SPINNING SPIDER SILK PROTEIN

The invention features methods and apparatuses for spinning silk protein fibers (biofilaments) from recombinant biofilament proteins. The methods are particularly useful for spinning fibers of spider silk or silkworm silk proteins from recombinant mammalian cells and may be used to spin such fibers for use in the manufacture of industrial and commercial products.

PRECURSOR FEEDSTOCK FOR FORMING FILAMENTS

The application relates to a method of obtaining silk feedstock solution from a gland of a silkworm or another silk-producing arthropod in which the body of the silkworm or the other silk-producing arthropod is cut open and the gland removed. The silk feedstock solution is subsequently extracted from the gland and used for extrusion, spinning or moulding of objects.

Method for Producing Fibroin-Like Protein

A method for producing a fibroin-like protein is provided. A fibroin-like protein is produced by a method of culturing having a gene encoding the fibroin-like protein in a medium, inducing expression of the gene encoding the fibroin-like protein, and collecting the fibroin-like protein, wherein accumulation of an organic acid at the time of inducing the expression is reduced, and wherein the gene is expressed under control of a tryptophan promoter. 1. A method for producing a fibroin-like protein , the method comprising:{'i': 'Escherichia coli', '(A) culturing having a gene encoding the fibroin-like protein in a medium;'}(B) inducing expression of the gene encoding the fibroin-like protein; and(C) collecting the fibroin-like protein,wherein accumulation of an organic acid at the time of said inducing the expression is reduced, andwherein the gene is expressed under the control of a tryptophan promoter.2. The method according to claim 1 , wherein said inducing occurs by depleting tryptophan in the medium claim 1 , adding 3-beta-indoleacrylic acid to the medium claim 1 , or a combination thereof.3. The method according to claim 1 , wherein said inducing occurs either by depleting tryptophan in the medium claim 1 , or by depleting tryptophan in the medium and adding 3-beta-indoleacrylic acid to the medium.4. The method according to claim 2 , wherein the concentration of tryptophan in the medium is lower than 50 mg/L during said depleting.5. The method according to claim 1 ,wherein the step (A) comprises a culture period A1 and a culture period A2,wherein the culture period A2 occurs after the culture period A1,wherein the concentration of tryptophan in the medium during the culture period A1 is 50 mg/L or higher, andwherein the concentration tryptophan in the medium during the culture period A2 is lower than 50 mg/L.6. The method according to claim 5 , wherein said inducing expression occurs at the timepoint of switching from the culture period A1 to the culture period ...

Methods of generating highly-crystalline recombinant spider silk protein fibers

Provided herein are scalable methods of processing wet-spun fiber comprising recombinant spider silk polypeptides to generate a three-dimensional crystalline lattice of beta-sheet structures in the fiber.

Systems and methods for manufacturing a silk fibroin solution and powders containing silk fibroin

The disclosure relates to systems and methods for improving the manufacturing of silk solutions and powders containing silk fibroin obtained from silkworm cocoons. The solutions and powders can be used to improve the post-harvest preservation of perishables and to improve the performance of packaging, including biodegradable packaging.

В ВЫСОКОЙ СТЕПЕНИ СЖАТОЕ СИНТЕТИЧЕСКОЕ ФИБРОИНОВОЕ ВОЛОКНО, СПОСОБ ЕГО ПОЛУЧЕНИЯ И СПОСОБ СЖАТИЯ СИНТЕТИЧЕСКОГО ФИБРОИНОВОГО ВОЛОКНА

ШТАПЕЛЬНЫЕ ВОЛОКНА ИЗ НАТУРАЛЬНЫХ ВОЛОКОН, СПОСОБ И АППАРАТ ДЛЯ ИХ ПРОИЗВОДСТВА

Raw material for bio-spinning casting

Raw material for bio-spinning and casting

Improvements in or relating to the production of filaments, yarns, threads and other articles from natural silk

Albumens and albuminoids, processes for making artificial silk and like textile materials from; albumens and albuminoids, films or sheets containing.-Filaments, threads, yarns, bristles and other articles are formed by dissolving natural silk in an acid, imparting to the solution the desired shape and then subjecting the solution to the action of a precipitating agent comprising one or more alcohols, e.g. ethyl or methyl alcohol. A strong acid is preferably employed, but not sufficiently strong to impair the silk at the temperature employed. Sulphuric acid of 25-75 per cent, syrupy orthophosphoric acid and hydrochloric acid are specified. To prevent deterioration, low temperatures are employed, e.g. from -10 DEG C. to 10 DEG C., and are preferably maintained during storage, if any, and precipitation. The solution is extruded to form light or heavy filaments. The precipitating bath is preferably of large volume and may include a diluent for the acid and/or neutralizing substances, e.g. sodium ...

A prosthetic fabric structure

Disclosed herein is an implantable prosthesis for breast augmentation or reconstruction procedures. The prosthesis comprises a biocompatible and biodegradable fabric structure comprising one or more individual yarns comprised of sericin-extracted native fibroin fibers, wherein the yarns are intertwined to produce the fabric structure. The fabric structure extends in at least a first dimension and has at least a first surface that is adapted to engage and support natural breast tissue and/or a prosthetic breast implant in a patient. Also disclosed are methods of supporting breast tissue or a breast implant in a patient by inserting the prosthesis between the skin of the patient and the breast tissue and/or implant within the patient.

NON-WOVEN FABRIC COMPRISING ULTRA-FINE FIBER OF SILK FIBROIN AND/OR SILK-LIKE MATERIAL, AND METHOD FOR PRODUCTION THEREOF

A non-woven fabric comprising ultra-fine fibers of silk fibroin and/or a silk- like material; and a method for producing the non-woven fabric, which comprises dissolving silk fibroin and/or a silk-like material in hexafluoroacetone hydrate or a solvent containing the hydrate as a primary component and subjecting the resultant solution to electrospinning.

SILK BIOMATERIALS AND METHODS OF USE THEREOF

The present invention provides an all-aqueous process and composition for production of silk biomaterials, e.g., fibers, films, foams and mats. In the process, at least one biocompatible polymer, such as poly(ethylene oxide) (PEO) (a well-documented biocompatible material), was blended with the silk protein prior to processing e.g., electrospinning. We discovered that this step avoids problems associated with conformational transitions of fibroin during solubilization and reprocessing from aqueous solution which lead to embrittled materials. Moreover, the process avoids the use of organic solvents that can pose problems when the processed biomaterials are exposed to cells in vitro or in vivo.

A NATURAL FIBER BASED STAPLE FIBERS, A METHOD AND APPARATUS FOR MANUFACTURING SUCH

The application relates to a method and apparatus for manufacturing a natural fiber based staple fibers. The application further relates to the staple fibers, staple fiber based raw wool and products comprising such. A method comprises providing a cellulose suspension (101, 310, 510) including water, refined cellulose fibrils and at least one rheology modifier, directing the cellulose suspension through a nozzle (102, 320, 520) onto a surface (300, 400, 500), drying the cellulose suspension onto the surface (103, 300, 400, 500) for forming a fiber (350, 550), and cutting the cellulose suspension on the surface for forming staple fibers (105).

MODIFIED FIBROIN

A modified fibroin including a domain sequence indicated by formula 1: [(A)nmotif-REP]m. The domain sequence has an amino acid sequence having a reduced (A)nmotif content compared to naturally-derived fibroin, said reduction being equivalent to the deletion of at least one (A)nmotif. [In formula 1, (A)nmotif indicates an amino acid sequence comprising 4-20 amino acid residues. The number of alanine residues relative to the total number of amino acid residues in (A)nmotif is at least 83%. REP indicates an amino acid sequence comprising 10-200 amino acid residues. m indicates an integer between 8 and 300. A plurality of (A)nmotif etc., may be the same amino acid sequence or different amino acid sequences.] ...

SPIDER SILK PROTEIN SCAFFOLDS FOR EUKARYOTIC CELL CULTURE

A method and a combination for the cultivation of eukaryotic cells are provided, as well as a method for preparation of eukaryotic cells. The methods comprise providing a sample of eukaryotic cells to be cultured, applying said sample to a cell scaffold material; and maintaining said cell scaffold material having cells applied thereto under conditions suitable for cell culture. The combination comprises eukaryotic cells and a cell scaffold material. The cell scaffold material comprises a polymer of a spider silk protein.

METHOD OF OBTAINING COLLAGEN SKIN OF HUMAN ORIGIN, FIBERS SO OBTAINED AND COMPOSITION CONTAINING THE SAME

PROCESS Of OBTAINING FIBRES OF COLLAGEN OF SKIN Of HUMAN ORIGIN, FIBRES THUS OBTAINED AND COMPOSITION the CONTAINER

METHOD FOR THE PREPARATION OF A NON-WOVEN SILK FIBROIN FABRICS

A process for producing non-woven silk fiber fabrics comprises the following steps: a) obtaining silk fibroin, for example either from silk cocoons, or silk textiles or waste silk; b) removing the sericin layer covering the silk fibroin fibers, when present; c) breaking the disulfide bonds between heavy (350 kDa) and light (27kDa) chains of silk fibroin in order to obtain the production of chain fragments which serve as a specific cellular recognition sites promoting the attachment and growth of cells. d) homogenising of the material resulting from step c).

PROCESS FOR COATING A BIOMEDICAL IMPLANT WITH A BIOCOMPATIBLE POLYMER AND A BIOMEDICAL IMPLANT THEREFROM

The present invention disclosed a process to coat the surface of flexible polymeric implant with biocompatible polymer such that the coating does not crack when the implant is subjected to mechanical forces such as tension, torsion or bending while retaining the inherent properties of the coated polymer. 1. A process for obtaining an implant with a biocompatible polymer coating , said process comprising:(a) dipping the implant in a solution of biocompatible polymer to obtain an implant with a dip coating; and 'wherein, fibres of the biocompatible polymer are partially embedded into the dip coating, and said coating remains intact/independent of cracks/tears even after application of mechanical forces selected from the group consisting of bending, tensile stress, compression, and torsion.', '(b) immediately electrospinning the same polymer onto the implant with dip coating of step (a) to obtain an implant with a biocompatible polymer coating;'}2. The process as claimed in claim 1 , wherein said biocompatible polymer is selected from the group consisting of silk fibroin claim 1 , polylactic acid (PLA) claim 1 , poly ε-caprolactone (PCL) claim 1 , and collagen.3. The process as claimed in claim 2 , wherein said biocompatible polymer is silk fibroin.4. The process as claimed in claim 1 , wherein said implant is made up of a material selected from the group consisting of metal claim 1 , polymer claim 1 , ceramic claim 1 , and composites thereof.5. The process as claimed in claim 3 , wherein said metal is titanium.6. The process as claimed in claim 3 , wherein said polymer is polydimethylsiloxane (PDMS) or polyethylene.7. The process as claimed in claim 3 , wherein said ceramic is hydroxyapatite.8. The process as claimed in claim 1 , wherein said implant is selected from the group consisting of breast implant claim 1 , ocular implant claim 1 , cardiovascular stent claim 1 , and catheter tube.9. The process as claimed in claim 1 , wherein said implant is in a form selected ...

RECOMBINANT-STRUCTURE PROTEIN MULTIFILAMENT AND METHOD FOR MANUFACTURING SAME

An object of the present invention is to provide a method for producing a modified fibroin multifilament excellent in productivity and a multifilament produced by the same. The multifilament of the present invention contains modified fibroin, in which the multifilament has 100 or more constituent single yarns, a coefficient of variation in elastic modulus is 15% or less, a coefficient of variation in strength is 15% or less, and a coefficient of variation in elongation is less than 33%. 1. A multifilament comprising modified fibroin , wherein the multifilament has 100 or more constituent single yarns , a coefficient of variation in elastic modulus is 15% or less , a coefficient of variation in strength is 15% or less , and a coefficient of variation in elongation is less than 33%.2. The multifilament according to claim 1 , wherein the coefficient of variation in elongation of the multifilament is 20% or less.3. The multifilament according to claim 1 , wherein the coefficient of variation in elongation of the multifilament is 10% or less.4. The multifilament according to claim 1 , wherein a coefficient of variation in fineness of the multifilament is 20% or less.5. The multifilament according to claim 1 , wherein the coefficient of variation in strength of the multifilament is 10% or less claim 1 , and the coefficient of variation in elastic modulus of the multifilament is 10% or less.6. The multifilament according to any one of claim 1 , wherein an average hydropathy index of the modified fibroin is more than −0.8.7. The multifilament according to claim 1 , wherein the modified fibroin is modified spider silk fibroin.8. The multifilament according to to claim 1 , wherein the multifilament has a shrinkage history of being irreversibly shrunk after spinning.9. The multifilament according to claim 8 , wherein the shrinkage history is a shrinkage history of being irreversibly shrunk by bringing the multifilament into contact with water or a shrinkage history of being ...

Molded Article and Method for Production Thereof

Disclosed is a molded article including a modified fibroin and a water resistance-imparting material. The modified fibroin and the water resistance-imparting material may be covalently bonded. 1. A molded article , comprising: a modified fibroin; and a water resistance-imparting material.2. The molded article according to claim 1 , wherein the modified fibroin and the water resistance-imparting material are covalently bonded.3. The molded article according to claim 1 , wherein the water resistance-imparting material is at least one selected from a silicone-based polymer and a fluorine-based polymer.4. The molded article according to claim 1 , wherein the modified fibroin is a modified spider silk fibroin.5. A method for producing a molded article claim 1 , the method comprising a step of bonding a water resistance-imparting material to a precursor molded article comprising a modified fibroin.6. The method according to claim 5 , wherein the step of bonding the water resistance-imparting material to the precursor molded article includes irradiating the precursor molded article with plasma in a state in which the water resistance-imparting material or a precursor of the water resistance-imparting material is brought into contact with the precursor molded article claim 5 , and thereby covalently bonding the modified fibroin and the water resistance-imparting material.7. The method according to claim 5 , wherein the water resistance-imparting material is at least one selected from a silicone-based polymer and a fluorine-based polymer.8. The method according to claim 5 , wherein the modified fibroin is a modified spider silk fibroin.9. A method for producing a molded article claim 5 , the method comprising a step of molding a raw material containing a modified fibroin and a water resistance-imparting material and thereby obtaining a molded article. The present invention relates to a molded article and a method for producing the same.Silk fiber is a natural fiber ...

Silk fibroin materials and use thereof

The present application provides a composition comprising porous silk fibroin scaffold material. The porous silk fibroin scaffold can be used for tissue engineering. The porosity of the silk fibroin scaffold described herein can be adjusted to mimic the gradient of densities found in natural tissue.

Method for Manufacturing Protein Fiber

The present invention relates to a method for manufacturing a protein fiber, the method including a step of bringing a spinning raw liquid containing a spider silk protein and a solvent into contact with a coagulation liquid to coagulate the spider silk protein, in which the coagulation liquid contains a ketone.

Process for making silk fibroin fibers

The present invention relates to a process for spinning silk fibers. The process involves dissolving silk fibroin in an aqueous salt solution, removing the salt from the solution, followed by removal of the water to form a regenerated silk material. The silk material is then dissolved in hexafluoroisopropanol to form a fiber-spinnable solution.

Electrospun silk material systems for wound healing

The present invention relates to the processes of preparing silkfibroin/polyethylene oxide blended materials, and the resulting materials thereof, which are suitable for biomedical applications such as wound healing. In particular, the electrospun silk fibroin/PEO mats with a silk:PEO blend ratio of 2:1 to 4:1, treated with controlled evaporation, constraint-drying techniques, and/or alcohol treatment, and/or PEO extraction, demonstrate suitable physical and biofunctional properties, such as fiber structure, topography, absorption, water vapor transmission rates, oxygen permeation, and biodegradability, relevant to biomaterial systems with utility for wound dressings.

Methods and combination

A method and a combination for the cultivation of eukaryotic cells are provided, as well as a method for preparation of eukaryotic cells. The methods comprise providing a sample of eukaryotic cells to be cultured, applying said sample to a cell scaffold material; and maintaining said cell scaffold material having cells applied thereto under conditions suitable for cell culture. The combination comprises eukaryotic cells and a cell scaffold material. The cell scaffold material comprises a polymer of a spider silk protein.

Microbe Proliferation Inhibitor, Method for Inhibiting Proliferation of Microbes, UV Resistance-Improving Agent, Method for Improving UV Resistance, Artificial Protein Formation Body and method for Manufacturing Same, Artificial Protein Solution, and Coloring Agent

One aspect of the present invention is a microbial growth inhibitor comprising xanthurenic acid. 1. (canceled)2. A method for inhibiting microbial growth in an article or improving UV resistance of an article , the method comprising incorporating xanthurenic acid in the article.3. The method according to claim 2 , wherein the article comprises a protein.4. The method according to claim 3 , wherein the protein is a structural protein.5. The method according to claim 4 , wherein the structural protein comprises fibroin.6. The method according to claim 5 , wherein the fibroin is spider silk fibroin.7. The method according to claim 2 , wherein the xanthurenic acid is incorporated at a ratio of 0.02 to 2.0% by mass with respect to a total mass of the article.8. The method according to claim 2 , wherein the article is fiber.916-. (canceled)17. An artificial protein formed product comprising:an artificial protein; andxanthurenic acid.18. The artificial protein formed product according to claim 17 , wherein the artificial protein is a structural protein.19. The artificial protein formed product according to claim 18 , wherein the structural protein comprises fibroin.20. The artificial protein formed product according to claim 19 , wherein the fibroin is spider silk fibroin.21. The artificial protein formed product according to claim 17 , wherein a content of the xanthurenic acid is 0.02 to 2.0% by mass with respect to a total mass of the artificial protein formed product.22. The artificial protein formed product according to claim 17 , wherein the artificial protein formed product is fiber.2327-. (canceled)28. An artificial protein solution comprising:an artificial protein; andxanthurenic acid.29. The artificial protein solution according to claim 28 , wherein the artificial protein is a structural protein.30. The artificial protein solution according to claim 29 , wherein the structural protein comprises fibroin.31. The artificial protein solution according to claim 30 , ...

Method for Producing Polymer Aggregate

The present invention relates to a method for producing a polymer aggregate, the method including: a step for bringing a polymer solution containing a polymer substance and cations for gel formation into contact with an anion solution containing anions for gel formation to form a gel at an interface between the polymer solution and the anion solution; and a step for causing the polymer substance to aggregate inside the gel. 1. A method for producing a polymer aggregate , comprising:a step for bringing a polymer solution containing a polymer substance and cations for gel formation into contact with an anion solution containing anions for gel formation to form a gel at an interface between the polymer solution and the anion solution; anda step for causing the polymer substance to aggregate inside the gel.2. The method for producing a polymer aggregate according to claim 1 , wherein the cations for gel formation are at least one selected from multivalent cations or hydrogen ions.3. The method for producing a polymer aggregate according to claim 1 , wherein the anions for gel formation are alginate ions.4. The method for producing a polymer aggregate according to claim 1 , wherein the anion solution further contains an aggregation promoter for the polymer substance.5. The method for producing a polymer aggregate according to claim 1 , wherein the polymer solution further contains a dissolution promoter for the polymer substance.6. The method for producing a polymer aggregate according to claim 1 , wherein solvents of the polymer solution and the anion solution are water.7. The method for producing a polymer aggregate according to claim 1 , wherein the polymer substance is a protein.8. The method for producing a polymer aggregate according to claim 7 , wherein the protein is a polypeptide derived from a spider silk protein. A computer readable text file, entitled “SequenceListing.txt,” created on or about Jun. 21, 2018 with a file size of about 60 kb contains the ...

Silk Fibroin Materials and Use Thereof

The present invention provides processes for producing porous silk fibroin scaffold material. The porous silk fibroin scaffold can be used for tissue engineering. The porosity of the silk fibroin scaffolds described herein can be adjusted as to mimic the gradient of densities found in natural tissue. Accordingly, methods for engineering of 3-dimensional tissue, e.g. bone and cartilage, using the silk fibroin scaffold material are also provided. 1. (canceled)2. A three-dimensional body comprising silk fibroin having interconnected pores , wherein the pores have an average diameter between about 50 microns and about 100 microns , and wherein total porosity of the three-dimensional body is at least 80%.3. The three-dimensional body of claim 2 , wherein the silk fibroin is in a beta-sheet conformation.4. The three-dimensional body of claim 2 , wherein the three-dimensional body comprises from 5% (w/v) to 35% (w/v) of the silk fibroin.5. The three-dimensional body of claim 2 , wherein the porosity is at least 90%.6. The three-dimensional body of claim 2 , wherein the porosity is at least 95%.7. The three-dimensional body of claim 2 , wherein the silk fibroin is substantially free of sericin.8. The three-dimensional body of claim 2 , wherein the three-dimensional body is an article for tissue engineering or tissue guided regeneration application.9. The three-dimensional body of claim 2 , wherein the three-dimensional body further comprises an additive.10. The three-dimensional body of claim 9 , wherein the additive is selected from peptides claim 9 , antibodies claim 9 , DNA claim 9 , RNA claim 9 , modified RNA/protein composites claim 9 , glycogens or other sugars and alcohols.11. The three-dimensional body of claim 9 , wherein the additive is a biologically active or pharmaceutically active compound.12. The three-dimensional body of claim 9 , wherein the additive is an agent that enhances proliferation or differentiation of a cell.13. The three-dimensional body of claim ...

PROTEIN FIBER CRIMPING METHOD, PROTEIN FIBER PRODUCTION METHOD, PROTEIN FIBERS, SPUN YARN, AND TEXTILE PRODUCT

Protein fiber having crimp property such that the protein fiber crimps in response to a stimulus is immersed in a solution of protein having a different composition from the protein. The protein having a different composition is infiltrated into the protein fiber and the protein fiber is made crimped. 1: A crimping method comprising:a step for immersing protein fiber having crimp property such that the protein fiber crimps in response to a stimulus, in a solution of protein having a different composition from the protein fiber; anda step for infiltrating said protein having a different composition into said protein fiber and crimping said protein fiber.2: The crimping method of claim 1 , wherein said protein fiber is made of artificial protein.3: The crimping method of claim 2 , wherein said artificial protein is artificial spider silk protein.4: The crimping method of claim 1 , wherein staples of filaments of said protein fiber are immersed in said solution of protein.5: The crimping method of claim 4 , wherein spun yarn made of said staples twisted together is immersed in said solution of protein.6: The crimping method of claim 5 , wherein a textile product made of said spun yarn is immersed in said solution of protein claim 5 , said staples in the textile product are made crimped claim 5 , and the textile product is fulled.7: The crimping method of claim 6 , wherein said textile product is immersed in said solution of protein under a condition that impact is not applied to said textile product.8: The crimping method of claim 1 , wherein said solution of protein is an aqueous solution of hydrolyzed keratin.9: The crimping method of claim 8 , wherein number averaged molecular weight of said hydrolyzed keratin is not less than 500 and not more than 5000.10: The crimping method of claim 8 , wherein concentration of said hydrolyzed keratin before immersing said textile product is not less than 0.1 mass % and not more than 2 mass % and immersing period of said textile ...

Molded Article, Production Method for Same, and Method for Improving Toughness of Molded Article

The present invention provides, in one aspect, a method for producing a molded article, the method comprising exposing a molded article precursor comprising a protein to an environment with a relative humidity of 90% or more to obtain the molded article. 1. A method for producing a molded article , the method comprising exposing a molded article precursor comprising a protein to an environment with a relative humidity of 90% or more to obtain the molded article.2. The method according to claim 1 , further comprising drying the molded article precursor before exposing the molded article precursor to the environment.3. The method according to claim 1 , wherein the protein is a structural protein.4. The method according to claim 1 , wherein the protein is at least one selected from the group consisting of keratin claim 1 , collagen claim 1 , elastin claim 1 , resilin claim 1 , silk fibroin claim 1 , and spider silk fibroin.5. The method according to claim 1 , wherein the protein is spider silk fibroin.6. A molded article comprising a protein having an exposure history to an environment with a relative humidity of 90% or more.7. The molded article according to claim 6 , wherein the protein is a structural protein.8. The molded article according to claim 6 , wherein the protein is at least one selected from the group consisting of keratin claim 6 , collagen claim 6 , elastin claim 6 , resilin claim 6 , silk fibroin claim 6 , and spider silk fibroin.9. The molded article according to claim 6 , wherein the protein is spider silk fibroin.10. A method for improving toughness of a molded article comprising a protein claim 6 , the method comprising exposing the molded article to an environment with a relative humidity of 90% or more.11. The method according to claim 10 , further comprising drying the molded article before exposing the molded article to the environment.12. The method according to claim 10 , wherein the protein is a structural protein.13. The method according to ...

Method and Device for Manufacturing Protein Fiber

The present invention relates to a method for manufacturing a protein fiber, including an extension and contraction step of contracting or extending a protein raw fiber containing a protein by bringing the protein raw fiber into contact with a liquid or vapor; and a drying step of drying the protein raw fiber that has undergone the extension and contraction step while adjusting a length of the protein raw fiber to an arbitrary length. 1. A method for manufacturing a protein fiber , comprising:an extension and contraction step of contracting or extending a protein raw fiber containing a protein by bringing the protein raw fiber into contact with a liquid or vapor; anda drying step of drying the protein raw fiber that has undergone the extension and contraction step while adjusting a length of the protein raw fiber to an arbitrary length.2. The method for manufacturing a protein fiber according to claim 1 , wherein the extension and contraction step is a step of naturally contracting the protein raw fiber by bringing the protein raw fiber into contact with the liquid or vapor.3. The method for manufacturing a protein fiber according to claim 2 , wherein the extension and contraction step is performed in a state where the protein raw fiber is not loosened.4. The method for manufacturing a protein fiber according to claim 3 , wherein claim 3 , in the extension and contraction step claim 3 , the protein raw fiber is tensioned such that a contraction amount of the protein raw fiber is substantially the same as a contraction amount of the protein raw fiber in a case where the protein raw fiber is naturally contracted by being brought into contact with the liquid or vapor in a state where the protein raw fiber is loosened.5. The method for manufacturing a protein fiber according to claim 1 , wherein the protein is a structural protein.6. The method for manufacturing a protein fiber according to claim 5 , wherein the structural protein is a spider silk fibroin.7. The method ...

Highly Contracted Synthetic Fibroin Fiber, Production Method Therefor, and Method for Contracting Synthetic Fibroin Fiber

The present invention relates to a highly contracted artificial fibroin fiber including a modified fibroin, in which a contraction percentage defined by the following equation exceeds 7%. 1. A highly contracted artificial fibroin fiber comprising:a modified fibroin, {'br': None, 'contraction percentage={1−(length of contracted artificial fibroin fiber/length of artificial fibroin fiber before being brought into contact with water after spinning)}×100(%)'}, 'wherein a contraction percentage defined by the following equation exceeds 7%.'}2. The highly contracted artificial fibroin fiber according to claim 1 , wherein the modified fibroin is a modified spider silk fibroin.3. The highly contracted artificial fibroin fiber according to claim 1 , which is contracted by being brought into contact with water below a boiling point.4. The highly contracted artificial fibroin fiber according to claim 3 , wherein a temperature of the water is 10° C. to 90° C.5. The highly contracted artificial fibroin fiber according to claim 3 , which is further contracted by drying after being brought into contact with the water.6. A method for producing a highly contracted artificial fibroin fiber claim 3 , comprising:a step of contracting an artificial fibroin fiber containing a modified fibroin by bringing the artificial fibroin fiber into contact with water below a boiling point, {'br': None, 'contraction percentage={1−(length of contracted artificial fibroin fiber/length of artificial fibroin fiber before being brought into contact with water after spinning)}×100(%)'}, 'wherein a contraction percentage defined by the following equation exceeds 7%.'}7. The method for producing a highly contracted artificial fibroin fiber according to claim 6 , wherein the modified fibroin is a modified spider silk fibroin.8. The method for producing a highly contracted artificial fibroin fiber according to claim 6 , wherein a temperature of the water is 10° C. to 90° C.9. The method for producing a highly ...

Production Method for Protein Molded Article, Production Method for Protein Solution, and Production Method for Protein

The present invention relates to a production method for a protein molded article, including: dissolving a protein in a solvent containing formic acid at a temperature of 40° C. or higher and lower than 80° C. to obtain a protein solution; and molding a protein molded article using the protein solution. 1. A production method for a protein molded article , comprising: dissolving a protein in a solvent containing formic acid at a temperature of 40° C. or higher and lower than 80° C. to obtain a protein solution; andmolding a protein molded article using the protein solution.2. The production method for a protein molded article according to claim 1 , wherein the protein molded article is a protein fiber.3. The production method for a protein molded article according to claim 1 , wherein the protein is a structural protein.4. The production method for a protein molded article according to claim 3 , wherein the structural protein is a spider silk fibroin.5. A production method for a protein solution claim 3 , comprising:dissolving a protein in a solvent containing formic acid at a temperature of 40° C. or higher and lower than 80° C. to obtain a protein solution.6. The production method according to claim 5 , wherein the protein is a structural protein.7. The production method for according to claim 6 , wherein the structural protein is a spider silk fibroin.8. A production method for a protein claim 6 , comprising:dissolving a target protein and impurities in a solvent containing formic acid at a temperature of 40° C. or higher and lower than 80° C. to obtain a protein solution containing the target protein; andtreating the protein solution with a poor solvent for the target protein to aggregate the target protein, thereby obtaining the target protein as an aggregate. The present invention relates to a production method for a protein molded article, a production method for a protein solution, and a production method for a protein.A fiber, a film, a porous body, and the ...

Blended Yarn, Knitted/Woven Body of Same, and Method for Manufacturing Said Knitted/Woven Body

The present invention aims at providing a blended yarn with which a knitted or woven body, wherein a decrease in tension and stiffness due to moistness is reduced, can be manufactured, even in a case where the blended yarn is used for the manufacture of the knitted or woven body; a knitted or woven body of the same; and a method for manufacturing the knitted or woven body. The blended yarn according to the present invention comprises a modified fibroin fiber and a regenerated cellulose fiber. 1. A blended yarn comprising:a modified fibroin fiber; anda regenerated cellulose fiber.2. The blended yarn according to claim 1 , wherein a shrinkage rate of the modified fibroin fiber as defined by the following formula is more than 7%:{'br': None, 'Shrinkage rate={1−(Length of modified fibroin fiber after being brought into contact with aqueous medium/Length of modified fibroin fiber before being brought into contact with aqueous medium)}×100(%).'}3. The blended yarn according to claim 1 , wherein an amount of the modified fibroin fiber is 5% to 40% by mass.4. The blended yarn according to claim 1 , wherein the modified fibroin fiber is a modified spider silk fibroin fiber.5. The blended yarn according to claim 1 , wherein the regenerated cellulose fiber is a lyocell fiber.6. A knitted or woven body of the blended yarn according to .7. A method for manufacturing a knitted or woven body claim 1 , comprising steps of:blend-spinning a modified fibroin fiber and a regenerated cellulose fiber to obtain a blended yarn; andknitting or weaving the blended yarn to obtain an unprocessed knitted or woven body.8. The manufacturing method according to claim 7 , wherein a shrinkage rate of the modified fibroin fiber as defined by the following formula is more than 7%:{'br': None, 'Shrinkage rate={1−(Length of modified fibroin fiber after being brought into contact with aqueous medium/Length of modified fibroin fiber before being brought into contact with aqueous medium)}×100(%).'}9. The ...

CONCENTRATED AQUEOUS SILK FIBROIN SOLUTION AND USE THEREOF

The present invention provides for concentrated aqueous silk fibroin solutions and an all-aqueous mode for preparation of concentrated aqueous fibroin solutions that avoids the use of organic solvents, direct additives, or harsh chemicals. The invention further provides for the use of these solutions in production of materials, e.g., fibers, films, foams, meshes, scaffolds and hydrogels. 158-. (canceled)59Bombyx mori. An aqueous silk fibroin solution having a fibroin concentration of at least 4 wt % , wherein said solution is fee of organic solvents , and wherein the silk fibroin is obtained from: a silkworm silk obtained from or a solution containing a genetically engineered silk.60. The aqueous silk fibroin solution of claim 59 , wherein the fibroin concentration is between 4 wt % to 20 wt %.61. The aqueous silk fibroin solution of claim 59 , the aqueous silk fibroin solution consisting of the silk fibroin and water.62. The aqueous silk fibroin solution of claim 59 , the aqueous silk fibroin solution further comprising an additive.63. A method comprising the steps of:(a) preparing a silk fibroin solution comprising silk fibroin in an aqueous salt solution; and(b) dialyzing said solution against a hygroscopic polymer to result in an aqueous silk fibroin solution of at least 4 wt % of fibroin.64. The method of claim 63 , further comprising the step of casting the aqueous silk fibroin solution to form a film.65. The method of claim 64 , wherein the casting includes air-drying.66. The method of claim 63 , wherein the dialyzing results in the aqueous silk fibroin solution having at least 10 wt % of fibroin.67. A silk fibroin composition having a fibroin concentration of at least 4% silk fibroin but not more than 33% silk fibroin claim 63 , wherein said composition is free of alcohol.68. The silk fibroin composition of claim 67 , wherein the silk fibroin composition comprises between about 10% and about 30% silk fibroin.69. The silk fibroin composition of claim 67 , ...

Method for Manufacturing Fibroin Solution and Method for Manufacturing Protein Molded Body

Disclosed is a method for manufacturing a fibroin solution including a step of forming a slurry containing a solvent-containing dissolving liquid and a fibroin-containing protein powder dispersed in the dissolving liquid by continuously introducing the protein powder to a thin film of the dissolving liquid while flowing the thin film; and a step of forming a fibroin solution by dissolving, in the dissolving liquid, the protein powder in the slurry. 1. A method for manufacturing a fibroin solution , the method comprising:a step of forming a slurry containing a solvent-containing dissolving liquid and a fibroin-containing protein powder dispersed in the dissolving liquid by continuously introducing the protein powder to a thin film of the dissolving liquid while flowing the thin film; anda step of forming a fibroin solution by dissolving, in the dissolving liquid, the protein powder in the slurry.2. The method according to claim 1 , wherein the thin film of the dissolving liquid is flowed on a surface by continuously supplying the dissolving liquid onto the surface.3. The method according to claim 1 , wherein the step of forming the slurry includes continuously supplying the dissolving liquid onto a rotation surface of a rotary body by using a metering pump so that the thin film of the dissolving liquid flows on the rotation surface claim 1 , and continuously supplying the protein powder by using a metering feeder so that the protein powder is continuously introduced to the thin film on the rotation surface.4. The method according to claim 1 , wherein the protein powder in the slurry is dissolved in the dissolving liquid by heating the slurry under stirring in an in-line mixer.5. The method according to claim 1 , further comprising a step of cooling the fibroin solution under stirring in the in-line mixer.6. The method according to claim 1 , further comprising a step of defoaming the fibroin solution.7. The method according to claim 1 , further comprising a step of ...

Composite materials comprising synthetic dragline spider silk

Composites based on a polymer and a mixture of proteins derived from a MaSp (major ampullate spidroin) protein are provides. Further, methods for preparation of same, and method of use of the composites are provided.

SHAPE MEMORY SILK MATERIALS

The present application relates to silk fibroin-based materials, methods for making and using the same. Provided materials exhibit shape memory characteristics while showing comparable or better volumetric swelling, biocompatibility and/or degradability when compared to current memory polymers derived from either natural or synthetic materials. 1. A silk material having a structure characterized by:interconnected, evenly spaced pores having rounded morphology;substantial absence of ice crystals;substantial uniformity of pore shape; orcombinations thereof, ["a) is susceptible to compression into a compressed state of reduced volume relative to the material's initial volume prior to compression; and", 'b) is characterized by an ability, in response to an environmental trigger, to expand from the compressed state to an expanded state having an expanded volume comparable to the initial volume., 'which silk material2. A silk material having a structure characterized in that it achieves a volume expansion of at least two fold and/or a mass increase of about 400% when exposed to a trigger to form a sponge having a structure characterized by:interconnected, evenly spaced pores having rounded morphology;substantial absence of ice crystals;substantial uniformity of pore shape; orcombinations thereof.3. The silk material of or , wherein the material is characterized by volumetric expansion when exposed to a trigger and/or external stimulus following compression.4. The silk material of claim 3 , wherein the swelling is at least two fold and/or about 400%.5. The silk material of claim 3 , wherein the trigger comprises one or more members selected from the group consisting of: an aqueous media claim 3 , heat claim 3 , electrical or magnetic fields claim 3 , pH claim 3 , light claim 3 , pressure claim 3 , ions claim 3 , enzymes and sugar.6. The silk material of claim 5 , wherein the trigger is water claim 5 , Phosphate Buffered Saline (“PBS”) claim 5 , Dulbecco's Modified Eagle's ...

METHOD AND APPARATUS FOR MANUFACTURING A STAPLE FIBER BASED ON NATURAL PROTEIN FIBER, A RAW WOOL BASED ON THE STAPLE FIBER, A FIBROUS YARN MADE OF THE STAPLE FIBER, A NON-WOVEN MATERIAL MADE OF THE STAPLE FIBER AND AN ITEM COMPRISING THE STAPLE FIBER.

Disclosed embodiments describe a method for manufacturing a staple fiber based on natural protein fiber. The method may include: providing a protein suspension, the protein suspension comprising fibrils of the natural protein fiber; directing the protein suspension through a nozzle onto a surface for forming a protein based fiber; drying the protein suspension on the surface; extracting the fiber from the surface; and providing the staple fiber. Disclosed embodiments may further describe a fiber based raw wool. The raw wool may include staple fibers, wherein the staple fibers are reconstructed on the basis of protein fibrils and are mechanically subdivided from natural protein fibers, wherein the protein fibrils are interlocked by hydrogen bonds, and wherein the raw wool comprises an unoriented, entangled, fluffy network of staple fibers. 148. -. (canceled)49. A method for manufacturing a staple fiber based on natural protein fiber , the method comprising:providing a protein suspension, the protein suspension comprising fibrils of the natural protein fiber;directing the protein suspension through a nozzle onto a surface for forming a protein based fiber;drying the protein suspension on the surface;extracting the fiber from the surface; andproviding the staple fiber.50. The method of claim 49 , wherein the natural protein fibers comprise collagen fibers.51. The method of claim 49 , wherein the natural protein fibrils are mechanically divided from a source of natural protein fibers.52. The method of claim 49 , wherein the protein suspension exits the nozzle in at least one of a round of elliptic cross-sectional shape.53. The method of claim 49 , wherein drying the protein suspension on the surface comprises drying via at least one of: radiation claim 49 , blowing claim 49 , and conduction.54. The method of claim 49 , wherein the surface comprises a hydrophobic external surface.55. The method of claim 49 , further comprising applying at least one of oil and wax to the ...

Modified Fibroin Fibers

The present invention provides a modified fibroin fiber having a shrinkage history of being irreversibly shrunk after spinning, the modified fibroin fiber containing modified fibroin, wherein a fiber diameter of a raw material fiber before being irreversibly shrunk exceeds 25 μm. 1. A modified fibroin fiber having a shrinkage history of being irreversibly shrunk after spinning , the modified fibroin fiber comprising modified fibroin , wherein a fiber diameter of a raw material fiber before being irreversibly shrunk exceeds 25 μm.2. The modified fibroin fiber according to claim 1 , wherein the shrinkage history is a shrinkage history of being irreversibly shrunk by bringing the raw material fiber into contact with water or a shrinkage history of being irreversibly shrunk by heating and relaxing the raw material fiber.3. The modified fibroin fiber according to claim 1 , wherein there is substantially no residual stress generated by drawing during a spinning process.4. The modified fibroin fiber according to claim 1 , wherein a shrinkage rate is 3.3% or less claim 1 , the shrinkage rate being defined by the following Equation (1):{'br': None, 'Shrinkage rate (%)=(1−(length of modified fibroin fiber when dried from wet state/length of modified fibroin fiber when in wet state))×100.'}5. The modified fibroin fiber according to claim 1 , wherein the modified fibroin is modified spider silk fibroin.6. The modified fibroin fiber according to claim 1 , wherein the modified fibroin is hydrophobic-modified spider silk fibroin.7. The modified fibroin fiber according to claim 1 , wherein the modified fibroin fiber has a fiber diameter of less than ±20% of the fiber diameter of the raw material fiber before being irreversibly shrunk.8. The modified fibroin fiber according to claim 1 , wherein a sectional shape is a circular shape or an elliptical shape.9. A product comprising the modified fibroin fiber according to .10. The product according to claim 9 , wherein the product is ...

IMPROVED SILK FIBERS

Methods and compositions are provided for improved proteinaceous block copolymer fibers based on long repeat units having molecular weight of greater than about 10 kDal. Each repeat unit includes more than about 150 amino acid residues that are organized into a number of “quasi-repeat units.” The fibers have improved mechanical properties that better recapitulate those of C the native silk fibers. 1. A proteinaceous block copolymer fiber , wherein the block copolymer comprises: more than 150 amino acid residues and having a molecular weight of at least 10 kDal;', 'an alanine-rich region with 6 or more consecutive amino acids, comprising an alanine content of at least 80%;', 'a glycine-rich region with 12 or more consecutive amino acids, comprising a glycine content of at least 40% and an alanine content of less than 30%; and, 'at least two occurrences of a repeat unit, the repeat unit comprisingwherein the fiber comprises at least one property selected from the group consisting of a modulus of elasticity greater than 550 cN/tex, an extensibility of at least 10% and an ultimate tensile strength of at least 15 cN/tex.2. The fiber of claim 1 , wherein the repeat unit comprises from 150 to 1000 amino acid residues.3. The fiber of any of - claim 1 , wherein the repeat unit has a molecular weight from 10 kDal to 100 kDal.4. The fiber of any of - claim 1 , wherein the repeat comprises from 2 to 20 alanine-rich regions.5. The fiber of any of - claim 1 , wherein each alanine-rich region comprises from 6 to 20 consecutive amino acids claim 1 , comprising an alanine content from 80% to 100%.6. The fiber of any of - claim 1 , wherein the repeat comprises from 2 to 20 glycine-rich regions.7. The fiber of any of - claim 1 , wherein each glycine-rich region comprises from 12 to 150 consecutive amino acids claim 1 , comprising a glycine content from 40% to 80%.8. The fiber of claim 1 , wherein the repeat unit comprises 315 amino acid residues claim 1 , 6 alanine-rich regions claim ...

Methods and Compositions for Synthesizing Improved Silk Fibers

The present disclosure provides methods and compositions for directed to synthetic block copolymer proteins, expression constructs for their secretion, recombinant microorganisms for their production, and synthetic fibers (including advantageously, microfibers) comprising these proteins that recapitulate many properties of natural silk. The recombinant microorganisms can be used for the commercial production of silk-like fibers. 1. An expression construct encoding a proteinaceous block co-polymer , comprising a polypeptide , the polypeptide comprising from 2 to 8 concatenated repeats of SEQ ID NO: 1396 or circularly permuted variants thereof.2. The expression construct of claim 1 , further comprising a secretion signal operatively linked to the coding sequence for the block co-polymer polypeptide.3. The expression construct of claim 2 , wherein the secretion signal comprises an alpha mating factor leader and pro sequence.4. The expression construct of claim 1 , wherein the polypeptide comprises a property selected from the group consisting of an alanine composition from 12 to 40% claim 1 , a glycine composition from 25 to 50% claim 1 , a proline composition from 9 to 20% claim 1 , a β-turn composition from 15 to 37% claim 1 , a GPG amino acid motif content from 18 to 55% claim 1 , and a poly-alanine amino acid motif content from 9 to 35%.5. The expression construct of claim 1 , the polypeptide consisting of 3 concatenated repeats of SEQ ID NO: 1396.6. A host cell claim 1 , comprising: the expression construct of .7. The host cell of claim 6 , wherein the cell is selected from the group consisting of: a yeast cell claim 6 , a fungal cell claim 6 , and a gram positive bacteria cell.8Trichoderma.. The host cell of claim 7 , wherein the cell is from the genus9. A method of producing a proteinaceous block co-polymer comprising: a polypeptide comprising from 2 to 8 concatenated repeats of SEQ ID NO: 1396 or circularly permuted variants thereof claim 7 , the method ...

POLAR SOLVENT SOLUTION AND PRODUCTION METHOD THEREOF

A polar solvent solution of the present invention is a polar solvent solution in which a solute containing a polyamino acid is dissolved in a polar solvent. The solution has a moisture content of less than 5 mass % based on 100 mass % of the solution. A method for producing a polar solvent solution of the present invention includes changing a moisture content of the solution to adjust the viscosity of the solution. Further, another method for producing a polar solvent solution includes reducing a moisture content of the solution to increase the viscosity of the solution. Thus, the present invention provides a polar solvent solution that enables stable spinning and casting without lowering its viscosity when used as dopes for spinning, film, etc., and methods for producing the same. 1. A polar solvent solution in which a solute containing a polyamino acid is dissolved in a polar solvent ,wherein the solution has a moisture content of less than 5% by mass based on 100% by mass of the solution.2. The polar solvent solution according to claim 1 , wherein the polyamino acid is a polypeptide.3. The polar solvent solution according to claim 1 , wherein the polar solvent contains at least one selected from the group consisting of dimethylsulfoxide (DMSO) claim 1 , N claim 1 ,N-dimethylformamide (DMF) claim 1 , N claim 1 ,N-dimethylacetamide (DMA) claim 1 , and N-methyl-2-pyrrolidone (NMP).4. The polar solvent solution according to claim 2 , wherein the polypeptide is a structural protein.5. The polar solvent solution according to claim 4 , wherein the structural protein includes a crystal region.6. The polar solvent solution according to claim 2 , wherein the polypeptide is a spider silk protein.7. A method for producing a polar solvent solution in which a solute containing a polyamino acid is dissolved in a polar solvent claim 2 , comprising:changing a moisture content of the solution to adjust a viscosity of the solution.8. A method for producing a polar solvent solution ...

Methods and Compositions for Synthesizing Improved Silk Fibers

The present disclosure provides methods and compositions for directed to synthetic block copolymer proteins, expression constructs for their secretion, recombinant microorganisms for their production, and synthetic fibers (including advantageously, microfibers) comprising these proteins that recapitulate many properties of natural silk. The recombinant microorganisms can be used for the commercial production of silk-like fibers. 1. A fiber spinning solution , comprisinga polypeptide, the polypeptide comprising from 2 to 8 concatenated repeats of SEQ ID NO: 1396 or circularly permuted variants thereof, the polypeptide further comprising a FLAG tag; anda spinning solvent, wherein said polypeptide is dissolved in said spinning solvent.2. The fiber spinning solution of claim 1 , wherein said spinning solvent comprises formic acid.3. The fiber spinning solution of claim 1 , wherein said spinning solvent is formic acid.4. The fiber spinning solution of claim 1 , wherein said fiber spinning solution comprises from 20-30% of said polypeptide by weight.5. The fiber spinning solution of claim 1 , wherein the FLAG tag is on the C-terminal end of the polypeptide.6. The fiber spinning solution of claim 1 , wherein said FLAG tag is a 3×FLAG tag.7. The fiber spinning solution of claim 1 , wherein said FLAG tag is encoded by a polynucleotide comprising SEQ ID NO: 1409.8. The fiber spinning solution of claim 1 , wherein the polypeptide comprises a property selected from the group consisting of an alanine composition from 12 to 40% claim 1 , a glycine composition from 25 to 50% claim 1 , a proline composition from 9 to 20% claim 1 , a β-turn composition from 15 to 37% claim 1 , a GPG amino acid motif content from 18 to 55% claim 1 , and a poly-alanine amino acid motif content from 9 to 35%.9. The fiber spinning solution of claim 1 , wherein the polypeptide comprises exactly 3 concatenated repeats of SEQ ID NO: 1396.10. A method of making a silk fiber claim 1 , comprising:{'claim-ref ...

Composite Fibers and Method for Manufacturing Same

Provided is a side-by-side type composite fiber having a latent crimping ability, including a first component containing a modified fibroin and a second component containing a structural protein, in which the first component and the second component are joined to each other. 1. A side-by-side type composite fiber having a latent crimping ability , comprising:a first component containing a modified fibroin; anda second component containing a structural protein,wherein the first component and the second component are joined to each other.2. The composite fiber according to claim 1 , wherein a composition ratio of the first component to the second component is 9:1 to 1:9 based on a mass of the composite fiber.3. The composite fiber according to claim 1 , wherein the structural protein is at least one selected from the group consisting of a silk fibroin claim 1 , a spider silk fibroin claim 1 , collagen claim 1 , resilin claim 1 , elastin claim 1 , and keratin.4. The composite fiber according to claim 1 , wherein the structural protein is at least one selected from the group consisting of a silk fibroin claim 1 , a spider silk fibroin claim 1 , and keratin.5. The composite fiber according to claim 1 , wherein the modified fibroin is a modified fibroin including a domain sequence represented by Formula 1: [(A)motif−REP] claim 1 , and{'sub': n', 'n, 'the domain sequence has an amino acid sequence in which a content of the (A)motif is reduced, the amino acid sequence being equivalent to an amino acid sequence in which at least one or a plurality of the (A)motifs are deleted, as compared with a naturally occurring fibroin,'}{'sub': n', 'n', 'n, 'in Formula 1, the (A)motif represents an amino acid sequence composed of 2 to 27 amino acid residues and the number of alanine residues with respect to a total number of amino acid residues in the (A)motif is 83% or more, the REP represents an amino acid sequence composed of 10 to 200 amino acid residues, m represents an integer of ...

Methods of Generating Highly-Crystalline Recombinant Spider Silk Protein Fibers

Provided herein are scalable methods of processing wet-spun fiber comprising recombinant spider silk polypeptides to generate a three-dimensional crystalline lattice of beta-sheet structures in the fiber. 1. A drawn fiber comprising silk polypeptide , wherein the fiber is generated by:dissolving a powder comprising the silk polypeptide into a solvent to generate a spin dope;extruding the spin dope into a coagulation bath to form a precursor fiber;collecting the precursor fiber without drawing the precursor fiber; anddrawing the precursor fiber over a hot surface to generate a drawn fiber,wherein the drawn fiber has a crystallinity index of at least 6% as measured using X-ray diffraction.2. The drawn fiber of claim 1 , wherein said silk polypeptide is a recombinant silk polypeptide.3. The drawn fiber of claim 2 , wherein said recombinant silk polypeptide comprises two or more repeat units of a proteinaceous block copolymer.4. The drawn fiber of claim 1 , wherein said silk polypeptide is a recombinant spider silk polypeptide.5. The drawn fiber of claim 4 , wherein the recombinant spider silk polypeptide is 18B (SEQ ID NO: 1).6. The drawn fiber of claim 1 , wherein the powder comprising the silk polypeptide is comprised of at least 60% silk polypeptide by weight claim 1 , at least 70% silk polypeptide by weight claim 1 , or at least 80% silk polypeptide by weight.7. (canceled)8. (canceled)9. The drawn fiber of claim 1 , wherein the drawn fiber is drawn over the hot surface at a draw ratio of at least 2× claim 1 , at least 3× claim 1 , at least 4× claim 1 , or at least 6×.1012.-. (canceled)13. The drawn fiber of claim 9 , wherein the draw ratio is computed by determining the draw ratio at failure.14. The drawn fiber of claim 13 , wherein determining the draw ratio at failure comprises determining the distribution of maximum elongation at break of one or more precursor fibers using an apparatus designed to draw the fiber over a hot surface while increasing the draw ratio ...

Protein Molded Article And Method For Producing Same, Protein Solution, And Protein Molded Article Plasticizer

The present invention relates to a protein molded article including a protein, in which an ionic liquid is included inside the protein molded article. The present invention also relates to a production method of the protein molded article, including a step of adding the ionic liquid to a molding material of the protein molded article. Further, the present invention relates to a protein solution including a protein, an ionic liquid, and a solvent. Further, the present invention also relates to a protein molded article plasticizer including an ionic liquid. 1. A protein molded article comprising a protein , wherein an ionic liquid is included inside the protein molded article.2. The protein molded article according to claim 1 , wherein the ionic liquid is dispersed inside the protein molded article.3. The protein molded article according to claim 1 , wherein the protein is a structural protein.4. The protein molded article according to claim 3 , wherein the structural protein is fibroin.5. The protein molded article according to claim 4 , wherein the fibroin is spider silk fibroin.6. The protein molded article according to claim 1 , wherein the ionic liquid is a hydrophobic ionic liquid.7. The protein molded article according to claim 1 , wherein the protein molded article is a fiber or a film.8. The protein molded article according to claim 1 , wherein a temperature at which a heat weight loss ratio of the ionic liquid is 10% by mass is 200° C. or higher.9. A production method of the protein molded article according to claim 1 , comprising a step of adding the ionic liquid to a molding material of the protein molded article.10. The production method according to claim 9 , wherein the step of adding the ionic liquid is a step of causing infiltration of the ionic liquid into the molding material of the protein molded article.11. The production method according to claim 9 , wherein the step of adding the ionic liquid is a step of mixing the molding material of the ...

NANOFIBERS COMPRISING FIBROIN AS WELL AS SYSTEM COMPRISING HYDROGEL AND SAID NANOFIBERS

Described herein are nanofibers including an outer membrane and a core, wherein the outer membrane is made of fibroin and the core is a biocompatible and biodegradable polymer. Also described herein are a method for obtaining the nanofibers and the use thereof to convey bioactive molecules and/or particles and/or cells and/or in the treatment of diseases. Also described herein are powdered nanofibers, optionally suspended in an aqueous solution, a hydrogel system including the powdered nanofibers, and the use of the powdered nanofibers and of the hydrogel system to convey bioactive molecules and/or particles and/or cells and/or in the treatment of diseases. 1. Nanofibers comprising an outer membrane and a core , wherein said outer membrane is made of fibroin and said core is a biocompatible and biodegradable polymer.2. The nanofibers according to claim 1 , wherein said fibroin is the natural or recombinant protein of silk.3. The nanofibers according to claim 1 , wherein said biocompatible and biodegradable polymer is a natural or synthetic polymer claim 1 , used alone or in combination.4. The nanofibers according to claim 1 , wherein said biocompatible and biodegradable polymer is a water-soluble polymer.5. The nanofibers according to claim 1 , wherein said biocompatible and biodegradable polymer is one or more polymers selected from the group comprising polyethylene oxide (PEO) claim 1 , polycaprolactone (PCL) claim 1 , hyaluronic acid claim 1 , gelatin claim 1 , collagen claim 1 , chitosan claim 1 , alginate claim 1 , albumin.6. The nanofibers according to claim 1 , wherein said biocompatible and biodegradable polymer is PEO.7. The nanofibers according to claim 1 , wherein said nanofibers have a diameter from 50 to 2000 nm.8. The nanofibers according to claim 7 , wherein said nanofibers have a diameter from 200 to 500 nm.9. The nanofibers according to claim 1 , wherein a thickness of said outer membrane is from 10 to 750 nm claim 1 , or from 20 to 250 nm.10222. ...

POLAR SOLVENT SOLUTION AND PRODUCTION METHOD THEREOF

A polar solvent solution of the present invention is a polar solvent solution in which a solute containing a polyamine acid is dissolved in a polar solvent. An inorganic salt is added to the solution, and a mole ratio of moisture to the inorganic salt in the solution is 2.5×m×n or less, where m represents the number of cations forming the inorganic salt and n represents a charge number of the cation. A production method of the present invention is a method for producing the above solution, including: changing at, least one of a moisture content and a content of the inorganic salt in the solution to adjust a viscosity of the solution. Thus, the present invention provides a polar solvent solution whose viscosity can be easily adjusted to a desired value and thus enables stable spinning and casting when used as dopes for spinning, film, etc., and methods for producing the same. 1. A polar solvent solution in which a solute containing a polyamino acid is dissolved in a polar solvent ,wherein an inorganic salt is added to the solution, anda mole ratio of moisture to the inorganic salt in the solution is 2.5×m×n or less, where m represents the number of cations forming the inorganic salt and n represents a charge number of the cation.2. The polar solvent solution according to claim 1 , wherein the polyamino acid is a polypeptide.3. The polar solvent solution according to claim 1 , wherein the solution has a moisture content of 0.6% by mass or more and 9.1% by mass or less based on 100% by mass of the solution.4. The polar solvent solution according to claim 1 , wherein the polar solvent contains at least one selected from the group consisting of dimethylsulfoxide (DMSO) claim 1 , N claim 1 ,N-dimethylformamide (DMF) claim 1 , N claim 1 ,N-dimethylacetamide (DMA) claim 1 , and N-methyl-2-pyrrolidone (NMP).5. The polar solvent solution according to claim 2 , wherein the polypeptide is a structural protein.6. The polar solvent solution according to claim 5 , wherein the ...

SOLUTION-DYED PROTEIN FIBER AND METHOD FOR PRODUCING SAME

A solution-dyed protein fiber of the present invention includes 0-100 mass % of silk fibroin and 100-0 mass % of a polypeptide derived from spider silk proteins when the protein fiber is assumed to be 100 mass %, wherein the solution-dyed protein fiber contains a solution-dyeing colorant. The fiber is obtained by: dissolving or dispersing a solution-dyeing colorant in a solvent used for a spinning solution or in dimethyl sulfoxide, thereby preparing a coloring liquid; adding a solvent to the coloring liquid in an amount necessary for a spinning solution; adding and dissolving protein powder into the solvent, thereby preparing a spinning solution; and subjecting the spinning solution to wet spinning or dry-wet spinning. Thereby, the present invention provides a low-cost solution-dyed protein fiber in which a solution-dyeing colorant is dispersed uniformly and that can exhibit bright color tone, and a method for producing the same. 1. A solution-dyed protein fiber comprising 0-100 mass % of silk fibroin and 100-0 mass % of a polypeptide derived from spider silk proteins when the protein fiber is assumed to be 100 mass % ,wherein the solution-dyed protein fiber contains a solution-dyeing colorant.2. The solution-dyed protein fiber according to claim 1 , wherein the solution-dyeing colorant is at least one selected from dyes and pigments.3. The solution-dyed protein fiber according to claim 1 , wherein the solution-dyeing colorant is at least one selected from an acid dye claim 1 , a basic dye claim 1 , a fluorescent dye claim 1 , a direct dye claim 1 , a disperse dye claim 1 , a plant pigment claim 1 , a food natural pigment claim 1 , and a carbon black.4. The solution-dyed protein fiber according to claim 1 , wherein an abundance of the solution-dyeing colorant is in a range of 0.1 to 2 mass % based on 100 mass % of the protein fiber.5. The solution-dyed protein fiber according to claim 1 , wherein the solution-dyed protein fiber has a diameter in a range of 5 to 200 ...

Modified Fibroin

Provided is a modified fibroin, including a domain sequence represented by Formula 1: [(A) n motif-REP] m , in which the domain sequence has an amino acid sequence having a reduced content of (A) n motif equivalent to an amino acid sequence in which, at least, one or a plurality of the (A) n motifs is deleted, as compared to naturally occurring fibroin. [In Formula 1, (A) n motif represents an amino acid sequence consisting of 4 to 20 amino acid residues and the number of alanine residues relative to the total number of amino acid residues in the (A) n motif is 83% or more, REP represents an amino acid sequence consisting of 10 to 200 amino acid residues, m represents an integer of 8 to 300, and a plurality of (A) n motifs or the like may be the same amino acid sequence or different amino acid sequences.]

Modified Fibroin

Provided is a modified fibroin, including a domain sequence represented by Formula 1: [(A)motif-REP], in which the domain sequence has an amino acid sequence having a reduced content of glycine residues equivalent to an amino acid sequence in which, at least, one or a plurality of the glycine residues in REP is substituted with another amino acid residue, as compared to naturally occurring fibroin: 1. A modified fibroin , comprising:{'sub': n', 'm, 'a domain sequence represented by Formula 1: [(A)motif-REP],'}wherein the domain sequence has an amino acid sequence having a reduced content of glycine residues equivalent to an amino acid sequence in which, at least, one or a plurality of the glycine residues in REP is substituted with another amino acid residue, as compared to naturally occurring fibroin;{'sub': n', 'n', 'n, 'wherein in Formula 1, (A)motif represents an amino acid sequence consisting of 4 to 20 amino acid residues and the number of alanine residues relative to the total number of amino acid residues in the (A)motif is 83% or more, REP represents an amino acid sequence consisting of 10 to 200 amino acid residues, m represents an integer of 8 to 300, a plurality of (A)motifs may be the same amino acid sequence or different amino acid sequences, and a plurality of REPs may be the same amino acid sequence or different amino acid sequences.'}2. The modified fibroin according to claim 1 , wherein the domain sequence has an amino acid sequence equivalent to an amino acid sequence in which claim 1 , at least claim 1 , in at least one motif sequence selected from GGX and GPGXX (where X represents an amino acid residue other than glycine) in REP claim 1 , one glycine residue in one or a plurality of the motif sequences is substituted with another amino acid residue claim 1 , as compared to the naturally occurring fibroin.3. The modified fibroin according to claim 2 , wherein the ratio of the motif sequence having the substitution of a glycine residue with ...

POLAR SOLVENT SOLUTION AND PRODUCTION METHOD THEREOF

A polar solvent solution of the present invention is a polar solvent solution in which a solute containing a polyamine acid is dissolved in a polar solvent. The solution has a moisture content of less than 5 mass % based on 100 mass % of the solution. A method for producing a polar solvent solution of the present invention includes changing a moisture content of the solution to adjust the viscosity of the solution. Further, another method for producing a polar solvent solution includes reducing a moisture content of the solution to increase the viscosity of the solution. Thus, the present invention provides a polar solvent solution that enables stable spinning and casting without lowering its viscosity when used as dopes for spinning, film, etc., and methods for producing the same. 16-. (canceled)7. A method for producing a polar solvent solution in which a solute containing a polyamino acid is dissolved in a polar solvent , comprising:changing a moisture content of the solution to adjust a viscosity of the solution.8. A method for producing a polar solvent solution in which a solute containing a polyamino acid is dissolved in a polar solvent , comprising:reducing a moisture content of the solution to increase a viscosity of the solution.9. The method for producing a polar solvent solution according to claim 7 , wherein the polyamino acid is a polypeptide.10. The method for producing a polar solvent solution according to claim 7 , wherein the solution has a moisture content of less than 5% by mass based on 100% by mass of the solution.11. The method for producing a polar solvent solution according to claim 7 , wherein the polar solvent contains at least one selected from the group consisting of dimethylsulfoxide (DMSO) claim 7 , N claim 7 ,N-dimethylformamide (DMF) claim 7 , N claim 7 ,N-dimethylacetamide (DMA) claim 7 , and N-methyl-2-pyrrolidone (NMP).1214-. (canceled)15. The method for producing a polar solvent solution according to claim 7 , wherein the moisture ...

METHOD FOR PRODUCING THREAD BUNDLE DERIVED FROM BAGWORM SILK THREADS SPUN ON THE SURFACE OF A BASE MATERIAL

A bagworm silk thread spun on a base material was conventionally difficult to be collected, and thus, cannot be utilized. An object of the present invention is to develop and provide a method in which such a bagworm silk thread is peeled off from a base material with a weak force without being damaged and is collected as a thread bundle. 1. A method for producing a thread bundle of a bagworm silk thread , comprising:wetting liquid applying process of applying a wetting liquid to the bagworm silk thread spun on the surface of a base material; andseparating the bagworm silk thread from the base material,wherein the wetting liquid is in a liquid form at least in the range of 20° C. or more and less than 30° C. under the atmospheric pressure, and is a pure substance or a mixture that does not damage, denature, or dissolve a fibroin protein which is a fiber component of the bagworm silk thread.2. The method according to claim 1 , further comprising a spinning process of placing a bagworm on the surface of the base material before the wetting liquid applying process and allowing the bagworm to spin the thread.3. The method according to claim 2 , further comprising a bagworm collection process of collecting the bagworm together with a nest after the spinning process and before the wetting liquid applying process.4. The method according to claim 1 , further comprising a washing process of washing the separated bagworm silk thread.5. The method according to claim 1 , further comprising a degumming process of degumming the separated bagworm silk thread.6. The method according to claim 1 , wherein the wetting liquid is a pure substance or a mixture that has a melting point of less than 20° C. and a boiling point of 30° C. or more and 300° C. or less.7. The method according to claim 1 , wherein the wetting liquid is an aqueous solution or an organic solvent.8. A method for collecting a bagworm silk thread spun on the surface of a base material claim 1 , comprising:applying a ...

USE OF AROMATIC ALCOHOL AS STRUCTURAL INDUCER AND METHOD FOR TREATING SILK FIBROIN

The present invention provides use of an aromatic alcohol as a structural inducer for silk fibroin, and also provides a method for treating silk fibroin. The method includes the following steps: bringing silk fibroin into contact with an aromatic alcohol-containing vapor or liquid to induce the structural transformation of silk fibroin, to obtain a water-insoluble silk fibroin, wherein the mechanical performance of the resulting silk fibroin material and the bonding strength between the silk fibroin material and a substrate material are over 50% higher than those of a silk fibroin material obtained by a traditional treatment method (induction with methanol, ethanol, and steam). 1. Use of an aromatic alcohol as a structural inducer for silk fibroin.3. The use according to claim 1 , the aromatic alcohol is in a liquid or vapor state.4. A treatment method for obtaining a water-insoluble silk fibroin material claim 1 , comprising steps of:bringing silk fibroin into contact with an aromatic alcohol-containing vapor or liquid to induce the structural transformation of silk fibroin, to obtain the water-insoluble silk fibroin material.6. The treatment method according to claim 4 , wherein the silk fibroin material is in a dry state and has a moisture content of not higher than 30%.7. The treatment method according to claim 4 , wherein the silk fibroin material is soaked in an aromatic alcohol-containing liquid or the silk fibroin material is fumigated with an aromatic alcohol-containing vapor.8. The treatment method according to claim 4 , wherein when an aromatic alcohol-containing liquid is used for treatment claim 4 , the treatment time is not less than 5 seconds; and the treatment temperature is 20-200° C.9. The treatment method according to claim 4 , wherein when an aromatic alcohol-containing vapor is used for treatment claim 4 , the treatment time is not less than 5 min claim 4 , and the treatment temperature is 25-200° C.10. The treatment method according to claim 4 ...

Shape Memory Silk Materials

The present application relates to silk fibroin-based materials, methods for making and using the same. Provided materials exhibit shape memory characteristics while showing comparable or better volumetric swelling, biocompatibility and/or degradability when compared to current memory polymers derived from either natural or synthetic materials. 194.-. (canceled)95. A silk material having a structure characterized by:(i) interconnected, evenly spaced pores having rounded morphology;(ii) substantial absence of negative imprints of ice crystals;(iii) substantial uniformity of pore shape; or(iv) combinations thereof,wherein the structure is indistinguishable from a comparison structure made by cooling a silk fibroin solution at a cooling rate between −10° C./min to −0.001° C./min to a freezing temperature of less than or equal to −20° C., andwherein the silk material is susceptible to compression into a compressed state of reduced volume relative to the material's initial volume prior to compression.96. The silk material of claim 95 , wherein the material is characterized by volumetric expansion when exposed to a trigger and/or external stimulus following compression.97. The silk material of claim 96 , wherein the volumetric expansion is at least two fold and/or about 400%.98. The silk material of claim 96 , wherein the trigger is water claim 96 , Phosphate Buffered Saline (“PBS”) claim 96 , Dulbecco's Modified Eagle's Medium (“DMEM”) claim 96 , bodily fluids claim 96 , or combinations thereof claim 96 ,wherein the material remains compressed when dry and expands after immersion into the aqueous media.99. The silk material of claim 95 , wherein the silk material comprises silk fibroin having an average molecular weight in a range between about 500 Da and about 3000 kDa.100. The silk material of claim 95 , wherein the silk material comprises silk fibroin at a concentration within a range of about 0.1% to about 50%.101. The silk material of further comprising a ...

Artificial Hair Fiber, Method for Manufacturing Same, and Artificial Hair

The present invention pertains to an artificial hair fiber that includes an artificial fibroin fiber containing a modified fibroin and that expands when being in a wet state and shrinks when being dried after the wet state.

COMPOSITE MOLDING COMPOSITION INCLUDING FIBROIN-LIKE PROTEIN, AND METHOD FOR PRODUCING COMPOSITE MOLDING COMPOSITION

Provided are a composite molding composition such as a composite film and a composite fiber that have properties such as an improved tensile strength, strain and toughness as a result of blending a peptide or polyamino acid having a β-sheet structure with a natural or artificially modified fibroin-derived protein; a method for producing such composite molding composition; and a method for improving the physical properties of a composite molding composition containing a fibroin-derived protein. 1. A composition comprising:a fibroin-derived protein; anda polypeptide and/or polyamino acid having a β-sheet structure.2. The composition according to claim 1 ,wherein the composition is a composite molding composition which is obtained by molding the fibroin-derived protein blended with the polypeptide and/or polyamino acid having the β-sheet structure.3. The composite molding composition according to claim 2 ,wherein the composite molding composition retains the β-sheet structure derived from the polypeptide and/or polyamino acid.4. The composite molding composition according to claim 2 ,wherein said composite molding composition is selected from a composite fiber, a composite film, a composite gel or a composite molded body.5. A composite molding composition formed of a fibroin-derived protein blended with a polypeptide and/or polyamino acid having a β-sheet structure claim 2 ,wherein the composite molding composition is selected from a composite fiber, a composite film and a composite gel, and is produced by a method comprising:(i) preparing a dope solution in which the fibroin-derived protein and the polypeptide and/or polyamino acid having the β-sheet structure are dissolved; and(ii) molding the composite molding composition from the dope solution.6. A pre-drawn composite molding composition claim 5 , being selected from a composite fiber or a composite film claim 5 , and produced by a method comprising the processes (i) and (ii) according to and(iii) drawing the ...

Modified Fibroin

The present invention relates to a modified fibroin including a domain sequence represented by Formula 1: [(A)motif-REP]or Formula 2: [(A)motif-REP]-(A)motif, in which the domain sequence has an amino acid sequence with a reduced content of a glutamine residue equivalent to an amino acid in which one or a plurality of glutamine residues in REP are deleted or substituted with other amino acid residues, as compared with a naturally occurring fibroin. [In Formula 1 and Formula 2, (A)motif represents an amino acid sequence consisting of 4 to 27 amino acid residues, and the number of alanine residues with respect to the total number of amino acid residues in the (A)motif is 80% or more. REP represents an amino acid sequence consisting of 10 to 200 amino acid residues. m represents an integer of 10 to 300. A plurality of (A)motifs may be the same amino acid sequence or different amino acid sequences.] 1. A modified fibroin , comprising:{'sub': n', 'm', 'n', 'm', 'n, 'a domain sequence represented by Formula 1: [(A)motif-REP]or Formula 2: [(A)motif-REP]-(A)motif,'}wherein the domain sequence has an amino acid sequence equivalent with a reduced content of a glutamine residue to an amino acid in which one or a plurality of glutamine residues in REP are deleted or substituted with other amino acid residues, as compared with a naturally occurring fibroin wherein{'sub': n', 'n', 'n, 'in Formula 1 and Formula 2, (A)motif represents an amino acid sequence consisting of 4 to 27 amino acid residues and the number of alanine residues with respect to the total number of amino acid residues in the (A)motif is 80% or more, REP represents an amino acid sequence consisting of 10 to 200 amino acid residues, m represents an integer of 10 to 300, a plurality of (A)motifs may be the same amino acid sequence or different amino acid sequences, and a plurality of REPs may be the same amino acid sequence or different amino acid sequences.'}2. The modified fibroin according to claim 1 , wherein ...

PEPTIDE-BASED NANOFIBRILLAR MATERIALS

Disclosed herein are nanofibrillar materials and aerogel-like materials comprised of nanofibrils, and methods for making such materials. 1. An nanofibrillar material , comprising: nanofibrils with an average diameter between about 2 nm and about 40 nm and', 'interconnected pores,', 'wherein a majority of the interconnected pores have an average diameter between about 2 nm and about 100 nm; and, 'an amphiphilic polypeptide having'} a porosity of at least about 80%,', {'sup': '3', 'a bulk density that is less than about 75 kg/m, and'}, 'a compressive modulus between that is between about 50 kPa and about 300 kPa., 'wherein the nanofibrillar material is characterized by'}2. The nanofibrillar material of claim 1 , wherein the amphiphilic polypeptide comprises a beta-sheet secondary structure.3. The nanofibrillar material of or claim 1 , wherein the amphiphilic polypeptide comprises an amino acid sequence of polypeptides selected from the group consisting of:actins, catenins, claudins, coilins, collagens, elastins, elaunins, extensins, fibrillins, fibroins, keratins, laminins, lamins, tublins, viral structural proteins, zein proteins (seed storage protein) or combination thereof.4. The nanofibrillar material of any of the preceding claims claim 1 , wherein the amphiphilic polypeptide comprises an amino acid sequence motif GAGAGS (SEQ ID NO: 1).5. The nanofibrillar material of claim 4 , wherein the polypeptide comprises a plurality of GAGAGS repeats.6. The nanofibrillar material of any of the preceding claims claim 4 , wherein the amphiphilic polypeptide comprises a silk fibroin polypeptide.7. The nanofibrillar material of any of the preceding claims claim 4 , wherein the nanofibrillar material consists essentially of a silk fibroin polypeptide.8. The nanofibrillar material of any of the preceding claims claim 4 , wherein the amphiphilic polypeptide has an average molecular weight between about 10 kDa and about 400 kDa.9. The nanofibrillar material of any of - claim 4 , ...

Methods and Compositions for Synthesizing Improved Silk Fibers

The present disclosure provides methods and compositions for directed to synthetic block copolymer proteins, expression constructs for their secretion, recombinant microorganisms for their production, and synthetic fibers (including advantageously, microfibers) comprising these proteins that recapitulate many properties of natural silk. The recombinant microorganisms can be used for the commercial production of silk-like fibers.

COMPRESSION AND HEAT-ASSISTED PRODUCTION OF SILK-BASED MATERIALS

In some embodiments, the present disclosure provides methods including the steps of (i) providing silk fibroin material comprising substantially amorphous structure, and (ii) applying at least one of elevated temperature and elevated pressure to the silk fibroin material to form a silk fibroin article, wherein the applying induces fusion between at least a portion of the silk fibroin and structural change of fibroin in the silk fibroin material. In some embodiments, the present disclosure also provides silk fibroin articles made in accordance with the methods disclosed herein. 1. A method comprising:providing silk fibroin material comprising substantially amorphous structure; and(ii) applying at least one of elevated temperature and elevated pressure to the silk fibroin material to form a silk fibroin article, wherein the applying induces fusion between at least a portion of the silk fibroin and structural change of fibroin in the silk fibroin material.2. A method comprising:selecting an elevated temperature and an elevated pressure to produce a desired silk fibroin article of a desired crystallinity and desired material properties; and(ii) applying the elevated temperature and the elevated pressure to a silk fibroin material comprising substantially amorphous structure to form a silk fibroin article, wherein the silk fibroin article has the desired crystallinity and the desired material properties.3. The method of claim 2 , wherein the applying step induces fusion between at least a portion of the filk fibroin and structural change of fibroin in the silk fibroin material.4. The method of any one of the above claims claim 2 , wherein the applying step comprises application of both elevated temperature and elevated pressure to the silk fibroin material.5. The method of any one of the above claims claim 2 , wherein the silk fibroin article is substantially homogeneous.6. The method of any one of the above claims claim 2 , wherein the silk fibroin article comprises ...

DEVICES AND METHODS FOR RADIATIVE COOLING

Devices for radiative cooling and optical waveguiding are provided, wherein the devices comprise a fabric including one or more fibers extending for a length in a longitudinal direction and a plurality of void structures positioned within each of the one or more fibers and extended over the length of each of the one or more fibers. Each of the plurality of void structures is configured to scatter at least a portion of an electromagnetic radiation received thereon to thereby radiatively cool the object. 1. A system for radiative cooling of an object positioned thereunder , comprising:a fabric including one or more fibers extending for a length in a longitudinal direction; anda plurality of void structures positioned within each of the one or more fibers and extended over the length of each of the one or more fibers, wherein each of the plurality of void structures is configured to scatter at least a portion of electromagnetic radiation received thereon to thereby radiatively cool the object.2. The system of claim 1 , wherein each of the plurality of void structures comprises a three-dimensional nanostructured void.3. The system of claim 1 , wherein each of the plurality of void structures comprises a void having a diameter from about 10 nm to about 10 μm.4. The system of claim 1 , wherein each of the plurality of void structures has a tubular shape.5. The system of claim 4 , wherein each of the tubular voids has a diameter from about 10 nm to about 10 μm claim 4 , and a length from 1 μm to 1 meter.6. The system of claim 4 , wherein each of the plurality of void structures scatters incident electromagnetic radiation at a wavelength to a transverse direction of the one or more fibers.7. The system of claim 1 , wherein the plurality of void structures includes void structures of a first size positioned in a center region of each of the one or more fibers claim 1 , and void structures of a second size positioned in an edge region of each of the one or more fibers.8. The ...

MOLDED REGENERATED SILK GEOMETRIES USING TEMPERATURE CONTROL AND MECHANICAL PROCESSING

The present disclosure provides methods for fabricating various regenerated silk geometries using temperature control. In addition to temperature control, mechanical processing can be used to enhance properties of the fabricated article. The present disclosure also provides silk foam and paper-like materials molded using freezer processing. 1. A method of fabricating an article from silk fibroin , the method comprising:(i) providing a silk fibroin solution into a mold; and(ii) holding the mold at a temperature from about −30° C. to about 25° C. for a period of time.2. (canceled)3. (canceled)4. (canceled)5. The method of claim 1 , further comprising a post-processing step.6. The method of claim 5 , wherein the post-processing step comprises drying claim 5 , rehydrating claim 5 , coating claim 5 , soaking in a solution claim 5 , mechanical processing claim 5 , or freeze-drying the article.7. The method of claim 1 , further comprising incubating the silk fibroin solution at a temperature from about −30° C. to about 25° C. for a period of time before coating the surface of the mold.8. The method of claim 1 , wherein the silk fibroin solution comprises an additive in addition to silk fibroins.9. (canceled)10. The method of claim 1 , wherein the article is a film claim 1 , a foam claim 1 , a fiber claim 1 , a coating claim 1 , a gel claim 1 , a hydrogel claim 1 , a sponge claim 1 , a 3D-scaffold claim 1 , and the like.11. The method of claim 1 , further comprising preprocessing the silk fibroin solution before contacting with the mold.12. The method of claim 11 , wherein said preprocessing comprises increasing viscosity of the silk fibroin solution.13. The method of claim 12 , wherein said preprocessing comprises electrogelation claim 12 , pH induced gelation claim 12 , shear stress induced gelation claim 12 , or a combination thereof.14. The method of claim 12 , further comprising heating the silk fibroin solution before pouring into the mold.15. An article prepared by a ...

CONCENTRATED AQUEOUS SILK FIBROIN SOLUTION AND USE THEREOF

The present invention provides for concentrated aqueous silk fibroin solutions and an all-aqueous mode for preparation of concentrated aqueous fibroin solutions that avoids the use of organic solvents, direct additives, or harsh chemicals. The invention further provides for the use of these solutions in production of materials, e.g., fibers, films, foams, meshes, scaffolds and hydrogels. 158-. (canceled)59. A composition comprising a silk fibroin protein solution and a therapeutic agent wherein said silk fibroin protein solution consists of at least 4% wt silk fibroin protein but not more than 33% wt silk fibroin protein.60. The composition of claim 59 , wherein said silk fibroin protein is obtained from a solution of dissolved silkworm silk claim 59 , genetically engineered silk claim 59 , or dissolved spider silk.61. The composition of claim 59 , wherein the therapeutic agent is a small molecule claim 59 , a protein claim 59 , a peptide claim 59 , a polysaccharide claim 59 , a glycoprotein claim 59 , a lipoprotein claim 59 , or a nucleic acid.62. The composition of claim 59 , wherein the therapeutic agent is a small molecule selected from the group consisting of vitamins claim 59 , sedatives claim 59 , hypnotics claim 59 , antibiotics claim 59 , antiviral agents claim 59 , steroids claim 59 , growth factor inhibitors claim 59 , growth factor promoters claim 59 , prostaglandins and radiopharmaceuticals.63. The composition of claim 59 , wherein the therapeutic agent is selected from the group consisting of anti-infectives claim 59 , chemotherapeutic agents claim 59 , anti-rejection agents claim 59 , analgesics and analgesic combinations claim 59 , anti-inflammatory agents claim 59 , hormones claim 59 , anti-angiogenic agents claim 59 , anti-thrombotics claim 59 , anti-metabolics claim 59 , antimitotics claim 59 , fibrinolytics claim 59 , and growth factors.64. The composition of claim 63 , wherein the growth factor is selected from the group consisting of bone ...

Methods and Compositions for Synthesizing Improved Silk Fibers

The present disclosure provides methods and compositions for directed to synthetic block copolymer proteins, expression constructs for their secretion, recombinant microorganisms for their production, and synthetic fibers (including advantageously, microfibers) comprising these proteins that recapitulate many properties of natural silk. The recombinant microorganisms can be used for the commercial production of silk-like fibers. 1. A fiber spinning solution , comprisinga polypeptide, the polypeptide comprising from 2 to 8 concatenated repeats of a recombinant silk polypeptide or circularly permuted variants thereof, the polypeptide further comprising a FLAG tag; anda spinning solvent, wherein said polypeptide is dissolved in said spinning solvent.2. The fiber spinning solution of claim 1 , wherein said spinning solvent comprises formic acid.3. The fiber spinning solution of claim 1 , wherein said spinning solvent is formic acid.4. The fiber spinning solution of claim 1 , wherein said fiber spinning solution comprises from 20-30% of said polypeptide by weight.5. The fiber spinning solution of claim 1 , wherein the FLAG tag is on the C-terminal end of the polypeptide.6. The fiber spinning solution of claim 1 , wherein said FLAG tag is a 3×FLAG tag.7. The fiber spinning solution of claim 1 , wherein said FLAG tag is encoded by a polynucleotide comprising SEQ ID NO: 1409.8. The fiber spinning solution of claim 1 , wherein the polypeptide comprises a property selected from the group consisting of an alanine composition from 12 to 40% claim 1 , a glycine composition from 25 to 50% claim 1 , a proline composition from 9 to 20% claim 1 , a β-turn composition from 15 to 37% claim 1 , a GPG amino acid motif content from 18 to 55% claim 1 , and a poly-alanine amino acid motif content from 9 to 35%.9. The fiber spinning solution of claim 1 , wherein said concatenated repeats comprise SEQ ID NO: 1396.10. A method of making a silk fiber claim 1 , comprising:{'claim-ref': {'@idref ...

Biomimetic Mechanical Tension Driven Fabrication of Nanofibrillar Architecture

This present disclosure provides methods for utilizing such forces in when generating nanofibrillar constructs with engineered morphology from the nano- to macro-scales. Using for example, a biopolymer silk fibroin as a base material, patterns an intermediate hydrogel were generated within a deformable mold. Subsequently, mechanical tension was introduced via either hydrogel contraction or mold deformation, and finally a material is reentrapped in this transformed shape via beta-sheet crystallization and critical point drying. Topdown engineered anchorages, cables, and shapes act in concert to mediate precision changes in nanofiber alignment/orientation and a macroscale form of provided nanofibrillar structure. An ability of this technique to engineer large gradients of nano- and micro-scale order, manipulate mechanical properties (such as plasticity and thermal transport), and the in-situ generation of 2D and 3D, multi-tiered and doped, nanofibrillar constructs was demonstrated. 1. An article of manufacture , comprising:a nanofibrillar architecture, comprising:a fibrillar or fibrous material;wherein the nanofibrillar architecture is substantially crystalline, and wherein the nanofibrillar architecture is characterized by birefringence.2. The article of manufacture of claim 1 , wherein the fibrillar or fibrous material is or comprises actin claim 1 , collagen claim 1 , elastins claim 1 , keratin claim 1 , myosin claim 1 , and/or silk.3. The article of manufacture of any of the preceding claims claim 1 , wherein the nanofibrillar architecture is two-dimensional.4. The article of manufacture of any of the preceding claims claim 1 , wherein the nanofibrillar architecture is three-dimensional.5. The article of manufacture of any of the preceding claims claim 1 , wherein the nanofibrillar architecture further comprises at least one additive claim 1 , agent claim 1 , and/or functional moiety.6. The article of manufacture of any of the preceding claims claim 1 , wherein ...

METHOD FOR PRODUCING ELONGATED STRUCTURES SUCH AS FIBERS FROM POLYMER SOLUTIONS BY STRAINING FLOW SPINNING

A method of molecular self-assembly is disclosed using two interacting streams that are allowed to interact and are subsequently forced through an orifice. A first stream of a dope solution of polymer molecules is extruded out of a capillary. The dope stream is surrounded by a focusing fluid which is miscible with the dope solution. The interaction between the jet of dope solution and surrounding focusing fluid creates hydrodynamic stretching and allows for extracting solvent from the dope solution. Concentrated polymers within the solution at stretched regions of the jet interact, and finally self-assembly takes place after the fluids are forced through the outlet of a converging nozzle. The formation of the structure can be optionally completed in a coagulating space. The structures thus obtained such as fibers or threads can be wound onto a mandrel. 1. A method of molecular self-assembly , comprising:extruding a stream of a dope solution of polymer molecules out of a capillary into a surrounding environment of a coaxially flowing focusing fluid miscible with the dope solution in a space limited by a convergent nozzle with an outlet that the dope and focusing fluid are forced to traverse;hydrodynamically stretching the extruded stream of polymer dope solution inside the convergent nozzle due to its interaction with the focusing fluid while simultaneously and selectively extracting solvent into the focusing fluid from the dope solution by molecular diffusion;wherein polymer concentration in the dope solution at a stretched region of the stream reaches a level such that contact among polymer molecules results in molecular self-assembly of the polymer molecules and a delayed stretching is imposed when the dope solution and the focusing fluid are forced through the nozzle outlet; andcontinuously extracting an elongated structure of self-assembled polymer molecules such as a fiber or a thread;wherein the capillary-nozzle system presents the following parameters:{'sub': ...

SILK FIBROIN SECURITY FIBERS CONTAINING SECURITY MARKERS AND A PROCESS FOR THE PREPARATION THEREOF

Present invention discloses loaded silk fibroin fibers as security features. Particularly, it provides security fiber comprising silk fibroin loaded with fluorescent chromophore or IR absorbing chromophore useful to combat counterfeiting. The invention discloses paper composition containing loaded silk fibroin fibers, provides method for authentication of paper embedded with security features. 1. A security fiber comprising silk fibroin loaded with markers selected from inorganic UV fluorescent chromophore and/or organic IR absorbing chromophore that is identified on exposure to said spectral wavelength , wherein said security fiber is useful to combat counterfeiting.2. The security fiber according to claim 1 , wherein the UV fluorescent chromophore is selected from inorganic material preferably Sodium yttrium Fluoride-oxide doped with Ytterbium and Thalium.3. The security fiber according to claim 1 , wherein the IR absorbing chromophore is selected from organic dye such as Dimethyl{4-[1 claim 1 ,7 claim 1 ,7-tris(4-dimethylaminophenyl)-2 claim 1 ,4 claim 1 ,6-heptatrienylidene]-2 claim 1 ,5-cyclohexadien-1-ylidene}ammonium perchlorate4. The security fiber according to claim 1 , wherein the silk fibroin is selected from natural silk fiber or regenerated silk fiber.5. The security fiber according to claim 1 , wherein the markers are loaded in the silk fibroin in the ratio ranging between 0.09-0.1 wt %.6. The security fiber according to claim 1 , wherein the fibers are micron size claim 1 , in the range of 10 microns to 200 microns and thickness of the fibers is in the range of 50 microns to 150 microns.7. The security fiber according to claim 1 , wherein a substrate was embedded with security fiber which can be identified on exposure to said spectral wavelength.8. The security fiber according to claim 7 , wherein the substrate is selected from textiles claim 7 , fabrics claim 7 , plastics claim 7 , security paper such as bank note paper claim 7 , passport paper claim ...

ELECTROACTIVE BIOPOLYMER OPTICAL AND ELECTRO-OPTICAL DEVICES AND METHOD OF MANUFACTURING THE SAME

A method of manufacturing a biopolymer optical device includes providing a polymer, providing a substrate, casting the polymer on the substrate, and enzymatically polymerizing an organic compound to generate a conducting polymer between the provided polymer and the substrate. The polymer may be a biopolymer such as silk and may be modified using organic compounds such as tyrosines to provide a molecular-level interface between the provided bulk biopolymer of the biopolymer optical device and a substrate or other conducting layer via a tyrosine-enzyme polymerization. The enzymatically polymerizing may include catalyzing the organic compound with peroxidase enzyme reactions. The result is a carbon-carbon conjugated backbone that provides polymeric “wires” for use in polymer and biopolymer optical devices. An all organic biopolymer electroactive material is thereby provided that provides optical functions and features. 126-. (canceled)27. An electroactive biopolymer optical device comprising:a biopolymer having an optical feature;wherein the biopolymer is a genetically altered biopolymer or a chemically modified biopolymer comprising an aromatic organic compound;wherein the biopolymer is characterized by an ability to form crosslinks between a plurality of aromatic organic compounds, andwherein the biopolymer is characterized as conductive at the aromatic organic compound crosslinks, so that the device has directed integration of electronic components.28. The device of claim 27 , wherein the biopolymer is a genetically altered or chemically modified silk polypeptide.29. The device of claim 27 , wherein the aromatic organic compound is tyrosine and the aromatic organic compound crosslinks are crosslinks between at least two tyrosine monomers.30. The device of claim 29 , wherein the tyrosine crosslinks form a carbon-carbon conjugated backbone.31. The device of claim 29 , wherein the tyrosine crosslinks form conjugated conduits along the biopolymer.32. The device of claim ...

SPLIT INTEIN MEDIATED PROTEIN POLYMERIZATION FOR MICROBIAL PRODUCTION OF MATERIALS

The present disclosure is directed to systems and methods for synthesizing a spidroin. In some embodiments, the methods comprise synthesizing a monomer in vivo in a heterologous host, the monomer comprising an N-terminus IntC domain and a C-terminus IntN domain, and post-translationally polymerizing the synthesized monomer via in vitro split-intein mediated polymerization. 1. A method for synthesizing a spidroin , the method comprising:{'sup': C', 'N, 'synthesizing a monomer in vivo in a heterologous host, the monomer comprising an N-terminus Intdomain and a C-terminus Intdomain; and'}post-translationally polymerizing the synthesized monomer via in vitro split-intein mediated polymerization.2. The method of claim 1 , wherein the heterologous host is a protein-expressing microbial host.3. The method of claim 1 , wherein the monomer is a silk amino acid sequence from a spider species.4. The method of claim 1 , wherein the synthesized spidroin has a molecular weight of at least about 300 kDa.5. The method of claim 1 , further comprising spinning the synthesized spidroin into fibers.6. The method of claim 5 , wherein the fibers have a tensile strength of from about 0.9 GPa to about 1.3 GPa.7. The method of claim 5 , wherein the fibers have an extensibility of from about 10% to about 25%.8. The method of claim 5 , wherein the fibers have a toughness of from about 60 MJ/mto about 170 MJ/m.9. The method of claim 5 , wherein the fibers have a β-sheet content of from about 35% to about 45%.10. A method for synthesizing a spidroin claim 5 , the method comprising:{'sup': 'N', 'synthesizing a seed protein in vivo in a heterologous host, the seed protein comprising a C-terminus Intdomain;'}{'sup': C', 'N, 'synthesizing a monomer in vivo in the heterologous host, the monomer comprising an N-terminus Intdomain and a C-terminus Intdomain; and'}co-translationally polymerizing the monomer via in vivo split-intein mediated polymerization.11. The method of claim 10 , wherein the ...

SILK FIBERS

Methods and compositions are provided for improved proteinaceous block copolymer fibers based on long repeat units having molecular weight of greater than about 10 kDal. Each repeat unit includes more than about 150 amino acid residues that are organized into a number of “quasi-repeat units.” The fibers have improved mechanical properties that better recapitulate those of the native silk fibers. 1. A proteinaceous block copolymer fiber , wherein the block copolymer comprises: more than 150 amino acid residues and having a molecular weight of at least 10 kDal;', 'an alanine-rich region with 6 or more consecutive amino acids, comprising an alanine content of at least 80%;', 'a glycine-rich region with 12 or more consecutive amino acids, comprising a glycine content of at least 40% and an alanine content of less than 30%; and, 'at least two occurrences of a repeat unit, the repeat unit comprisingwherein the fiber comprises at least one property selected from the group consisting of a modulus of elasticity greater than 550 cN/tex, an extensibility of at least 10% and an ultimate tensile strength of at least 15 cN/tex.2. The fiber of claim 1 , wherein the repeat unit comprises from 150 to 1000 amino acid residues.3. The fiber of claim 1 , wherein the repeat unit has a molecular weight from 10 kDal to 100 kDal.4. The fiber of claim 1 , wherein the repeat comprises from 2 to 20 alanine-rich regions.5. The fiber of claim 1 , wherein each alanine-rich region comprises from 6 to 20 consecutive amino acids claim 1 , comprising an alanine content from 80% to 100%.6. The fiber of claim 1 , wherein the repeat unit comprises from 2 to 20 glycine-rich regions.7. The fiber of any of claim 1 , wherein each glycine-rich region comprises from 12 to 150 consecutive amino acids claim 1 , comprising a glycine content from 40% to 80%.8. The fiber of claim 1 , wherein the repeat unit comprises 315 amino acid residues claim 1 , 6 alanine-rich regions claim 1 , and 6 glycine-rich regions ...

Concentrated aqueous silk fibroin solution and use thereof

The present invention provides for concentrated aqueous silk fibroin solutions and an all-aqueous mode for preparation of concentrated aqueous fibroin solutions that avoids the use of organic solvents, direct additives, or harsh chemicals. The invention further provides for the use of these solutions in production of materials, e.g., fibers, films, foams, meshes, scaffolds and hydrogels.

Recombinant spider silk proteins

The present invention is directed to recombinant spider silk proteins, nucleic acids, coding for these recombinant spider silk proteins, as well as hosts suitable for expressing those nucleic acids. Furthermore, the present invention is directed to a method of aggregation of spider silk proteins and the use of the proteins in the field of biotechnology and/or medicine and other industrial fields, in particular in the manufacture of automotive parts, in the aircraft construction, in the processing of textiles and leather, as well as in the manufacture and processing of paper and the like.

Methods and compositions for synthesizing improved silk fibers

The present disclosure provides methods and compositions for directed to synthetic block copolymer proteins, expression constructs for their secretion, recombinant microorganisms for their production, and synthetic fibers (including advantageously, microfibers) comprising these proteins that recapitulate many properties of natural silk. The recombinant microorganisms can be used for the commercial production of silk-like fibers.

Artificial polypeptide fiber and method for producing same

This artificial polypeptide fiber is an artificial fiber having a polypeptide as the primary component, and has a stress of at least 350 MPa and a toughness of at least 138 MJ/m 3 . A method for producing an artificial polypeptide fiber is for producing the aforementioned artificial polypeptide fiber obtained by spinning a spinning solution (6) containing a polypeptide derived from natural spider silk protein and then stretching in at least two stages, and the at least two stages of stretching include a first stretching stage (3) in moist heat, and a second stretching stage (4) in dry heat. As a result, provided are: an artificial polypeptide fiber having high toughness and favorable stress and rupture elongation; and a method for producing the artificial polypeptide fiber.

SYSTEMS AND METHODS FOR MANUFACTURING A SILK FIBROIN SOLUTION AND POWDERS CONTAINING SILK FIBROIN

The disclosure relates to systems and methods for improving the manufacturing of silk solutions and powders containing silk fibroin obtained from silkworm cocoons. The solutions and powders can be used to improve the post-harvest preservation of perishables and to improve the performance of packaging, including biodegradable packaging. 1. A method of processing silk inputs to obtain food grade silk fibroin , the method comprising the steps of:introducing a plurality of silk inputs to a single reactor vessel;introducing a solvent to the single reactor vessel;introducing a first compound to the single reactor vessel;introducing heat to the single reactor vessel contents to promote degumming of the silk inputs;controlling movement or positioning of the silk inputs within the single reactor vessel;removing at least a portion of the solvent and any degumming residue from the single reactor vessel;rinsing the degummed silk inputs;introducing a second compound to the single reactor vessel to dissolve any remaining silk fibroin proteins into solution;agitating the contents of the single reactor vessel;filtering the contents of the single reactor vessel to substantially remove the second compound and produce a purified silk fibroin-based solution; andpowderizing the purified silk fibroin-based solution to obtain the purified silk fibroin in a powder form.2. The method of claim 1 , wherein the silk inputs come from a Bombyx mori silkworm.3. The method of claim 1 , wherein a packing density of the silk inputs in the single reactor vessel is between about 1% and about 70%.4. The method of claim 1 , wherein a packing density of the silk inputs in the single reactor vessel is greater than 5%.5. The method of claim 1 , wherein a packing density of the silk inputs in the single reactor vessel is greater than 15%.6. The method of claim 1 , wherein a packing density of the silk inputs in the single reactor vessel is greater than 25%.7. The method of claim 1 , wherein the filtering ...

High Molecular Weight Recombinant Silk or Silk-like Proteins and Micro or Nano-spider Silk or Silk-like Fibres Manufactured by Using the Same

본 발명은 천연 실크 단백질과 거의 유사한 분자량을 가지는 고분자량의 재조합 실크 또는 실크 유사 단백질 및 이를 이용하여 제조된, 물성이 향상된 마이크로 또는 나노 크기의 거미줄 또는 거미줄 유사 섬유에 관한 것이다. 본 발명에 따른 재조합 실크 또는 실크 유사 단백질은 거미로부터 생산되는 드래그라인 실크 단백질처럼 고분자량을 가지면서 섬유로 제조 시 천연 실크단백질과 유사하거나 더 우수한 물성을 가지는 장점이 있다. 따라서, 본 발명에 따른 재조합 실크 또는 실크 유사 단백질 및 이를 이용하여 제조된 거미줄 또는 거미줄 유사 섬유는 거미줄 섬유의 적용이 기대되는 생물공학적 분야 및/또는 의약 분야 등과 같은 다양한 산업적 분야에 사용될 수 있어 매우 유용하다. The present invention relates to a high molecular weight recombinant silk or silk-like protein having a molecular weight almost similar to that of a natural silk protein and to micro or nano-sized spider webs or spider webs having improved physical properties. Recombinant silk or silk-like protein according to the present invention has a high molecular weight, such as dragline silk protein produced from the spider has the advantage of having properties similar to or better than natural silk protein when manufactured from fibers. Therefore, the recombinant silk or silk-like protein and the cobweb or cobweb-like fiber prepared using the same according to the present invention can be used in various industrial fields such as biotechnological field and / or medicine field where application of cobweb fiber is expected. Do.

重组蜘蛛丝蛋白

本发明涉及重组蜘蛛丝蛋白，编码这些重组蜘蛛丝蛋白的核酸，以及适合于表达那些核酸的宿主。另外，本发明涉及聚集蜘蛛丝蛋白的方法和将所述蛋白质用在生物技术和/或药物领域和其它工业领域，特别是在制备汽车部件，在飞行器构建，在加工纺织品和皮革，以及在制备和加工纸等中的应用。

improved silk fiber

분자량이 약 10 kDal 초과인 긴 반복 단위에 기초한 개선된 단백질성 블록 공중합체 섬유에 대한 방법 및 조성물이 제공된다. 각각의 반복 단위는 다수의 "준-반복 단위"로 조직된 약 150개 초과의 아미노산 잔기를 포함한다. 섬유는 천연 실크 섬유의 기계적 특성을 보다 우수하게 재현하는 개선된 기계적 특성을 갖는다. Methods and compositions are provided for improved proteinaceous block copolymer fibers based on long repeat units having a molecular weight greater than about 10 kDal. Each repeat unit comprises more than about 150 amino acid residues organized into multiple “quasi-repeat units”. The fibers have improved mechanical properties that better reproduce the mechanical properties of natural silk fibers.

Composite material containing synthetic spider dragline silk

개선된 실크 섬유

분자량이 약 10 kDal 초과인 긴 반복 단위에 기초한 개선된 단백질성 블록 공중합체 섬유에 대한 방법 및 조성물이 제공된다. 각각의 반복 단위는 다수의 "준-반복 단위"로 조직된 약 150개 초과의 아미노산 잔기를 포함한다. 섬유는 천연 실크 섬유의 기계적 특성을 보다 우수하게 재현하는 개선된 기계적 특성을 갖는다.

Modified fibroin

A kind of nanometer silver antimicrobial regenerated silk fibroin material and preparation method thereof

本发明公开了一种纳米银抗菌再生丝素蛋白材料及其制备方法，包括以下步骤： 将脱胶蚕丝或再生丝素蛋白溶解于以甲酸为主的溶剂中获得丝素甲酸溶液，然后加入银盐搅拌溶解；

Metalloprotein compositions

Artificial polypeptide fiber and method for producing same

本发明的人造多肽纤维为以多肽为主成分的人造纤维，其应力为350MPa以上、韧性为138MJ/m 3 以上。本发明的人造多肽纤维的制造方法中，所述人造多肽纤维是通过将含有天然型蛛丝蛋白来源的多肽的纺丝液（6）纺丝，进行至少两阶段的拉伸而得到的，其中，所述至少两阶段的拉伸包括湿热下的第一阶段拉伸（3）和干热下的第二阶段拉伸（4）。由此，本发明提供应力和断裂伸长率适度、韧性高的人造多肽纤维及其制造方法。

Method for manufacturing protein crimped staples

本発明は、タンパク質フィラメントからタンパク質捲縮ステープルを効率的に且つ低コストに製造する法を提供することを目的とする。本発明に係るタンパク質捲縮ステープルの製造方法は、ａ）改変フィブロインを含む人工フィブロインフィラメントを準備する工程と、ｂ）人工フィブロインフィラメントをカットして、人工フィブロインステープルを得るカット工程と、ｃ）カット工程に先だって、人工フィブロインフィラメントを水性媒体と接触させて捲縮させるか、若しくはカット工程の後に、人工フィブロインステープルを水性媒体と接触させて捲縮させる捲縮工程とを含む。 An object of the present invention is to provide a method for efficiently and inexpensively producing protein crimp staples from protein filaments. The method for producing a protein crimped staple according to the present invention includes a) a step of preparing an artificial fibroin filament containing modified fibroin, b) a cutting step of cutting the artificial fibroin filament to obtain an artificial fibroin table, and c) cutting. Prior to the step, the artificial fibroin filament is brought into contact with an aqueous medium to be crimped, or after the cutting step, the artificial fibroin protein is brought into contact with an aqueous medium to be crimped.

Wound dressing of silk fibroin nanofibers nonwoven and its preparation

견섬유에서 세리신을 제거하여 얻어진 견 피브로인의 나노섬유가 부직포 형태로 서로 얽혀 있는 구조를 갖는 창상피복재가 제공된다. 상기 창상피복재는 견섬유에서 세리신을 제거하여 얻어진 견 피브로인 용액을 동결건조하고, 건조된 견 피브로인을 전기방사 용매에 용해시킨 후 전기 방사함으로써 제조될 수 있다. 본 발명의 창상피복재는 밀착성 및 공기투과도가 우수하고, 손상된 피부조직의 세포 재생에 유리하다. Provided is a wound dressing having a structure in which nanofibers of silk fibroin obtained by removing sericin from silk fibers are entangled with each other in the form of a nonwoven fabric. The wound coating material may be prepared by lyophilizing a silk fibroin solution obtained by removing sericin from silk fibers, dissolving the dried silk fibroin in an electrospinning solvent and then electrospinning. The wound dressing of the present invention has excellent adhesion and air permeability, and is advantageous for cell regeneration of damaged skin tissue.

Method and device for producing a thread from silk proteins

本发明涉及从丝蛋白质制备线的方法，包括适于实施所述方法的装置。而且，本发明涉及由此获得的线及其应用。本发明使用扩散单元，所述扩散单元导致以高收率生产高品质的丝线。

High-shrinkage man-made fibroin fiber and its production method, and shrinkage method of man-made fibroin fiber

【課題】充分に高い収縮率を有し、肌触り性及び柔軟性に優れ、しかも安全に製造が可能な高収縮人造フィブロイン繊維の提供。【解決手段】改変フィブロインを含む、収縮された人造フィブロイン繊維であって、下記式で定義される収縮率が２％以上である、高収縮人造フィブロイン繊維。収縮率＝｛１−（水に接触させて湿潤状態にした人造フィブロイン繊維の長さ／紡糸後、水と接触する前の人造フィブロイン繊維の長さ）｝×１００（％）【選択図】なし

A kind of sulfonic acid based quantum dot/fibroin albumen composite nano-fiber membrane and its preparation method and application

本发明提供了一种磺酸基量子点/丝素蛋白复合纳米纤维膜及其制备方法和应用，该制备方法包括以下过程：对天然蚕丝进行脱胶处理，获得丝素蛋白；接着溴化锂水溶液溶解丝素，并经过透析、离心以及冷冻干燥处理得到丝素蛋白海绵；将丝素蛋白海绵溶解在甲酸中混和均匀，然后将磺化石墨烯量子点水溶液通过超声均匀分散在丝素蛋白‑甲酸溶液中，并通过静电纺丝设备制备了上述复合纳米纤维膜；并利用小鼠成纤维细胞对制备的复合纳米膜进行了细胞活性评估，体现了复合纳米膜具有优异的生物相容性。本发明所制备的复合纤维膜具有良好的生物相容性、可降解性，同时具备促进人骨髓基质干细胞增殖分化的潜能，具有良好的生物医用材料应用前景。

Proteins with repeated sequences of polyamino acids or polypeptides, and chemical synthesis methods thereof

Concentrated aqueous silk fibroin solution and use thereof

The present invention provides for concentrated aqueous silk fibroin solutions and an all-aqueous mode for preparation of concentrated aqueous fibroin solutions that avoids the use of organic solvents, direct additives, or harsh chemicals. The invention further provides for the use of these solutions in production of materials, e.g., fibers, films, foams, meshes, scaffolds and hydrogels.

Concentrated aqueous silk fibroin solutions and their use

Concentrated aqueous silk fibroin solution and use thereof

Concentrated aqueous silk fibroin solutions free of organic solvents and uses thereof

The present invention provides for concentrated aqueous silk fibroin solutions and an all-aqueous mode for preparation of concentrated aqueous fibroin solutions that avoids the use of organic solvents, direct additives, or harsh chemicals. The invention further provides for the use of these solutions in production of materials, e.g., fibers, films, foams, meshes, scaffolds and hydrogels.

Concentrated aqueous silk fibroin solution and use thereof

The present invention provides for concentrated aqueous silk fibroin solutions and an all-aqueous mode for preparation of concentrated aqueous fibroin solutions that avoids the use of organic solvents, direct additives, or harsh chemicals. The invention further provides for the use of these solutions in production of materials, e.g., fibers, films, foams, meshes, scaffolds and hydrogels.

Concentrated aqueous silk fibroin solution and use thereof

The present invention provides for concentrated aqueous silk fibroin solutions and an all-aqueous mode for preparation of concentrated aqueous fibroin solutions that avoids the use of organic solvents, direct additives, or harsh chemicals. The invention further provides for the use of these solutions in production of materials, e.g., fibers, films, foams, meshes, scaffolds and hydrogels.

Concentrated aqueous silk fibroin solution and use thereof

The present invention provides for concentrated aqueous silk fibroin solutions and an all-aqueous mode for preparation of concentrated aqueous fibroin solutions that avoids the use of organic solvents, direct additives, or harsh chemicals. The invention further provides for the use of these solutions in production of materials, e.g., fibers, films, foams, meshes, scaffolds and hydrogels.

Concentrated aqueous silk fibroin solution and use thereof

The present invention provides for concentrated aqueous silk fibroin solutions and an all-aqueous mode for preparation of concentrated aqueous fibroin solutions that avoids the use of organic solvents, direct additives, or harsh chemicals. The invention further provides for the use of these solutions in production of materials, e.g., fibers, films, foams, meshes, scaffolds and hydrogels.

Concentrated aqueous silk fibroin solution and use thereof

The present invention provides for concentrated aqueous silk fibroin solutions and an all-aqueous mode for preparation of concentrated aqueous fibroin solutions that avoids the use of organic solvents, direct additives, or harsh chemicals. The invention further provides for the use of these solutions in production of materials, e.g., fibers, films, foams, meshes, scaffolds and hydrogels.

Concentrated aqueous silk fibroin solution and use thereof

The present invention provides for concentrated aqueous silk fibroin solutions and an all-aqueous mode for preparation of concentrated aqueous fibroin solutions that avoids the use of organic solvents, direct additives, or harsh chemicals. The invention further provides for the use of these solutions in production of materials, e.g., fibers, films, foams, meshes, scaffolds and hydrogels.

Concentrated aqueous silk fibroin solution and use thereof

The present invention provides for concentrated aqueous silk fibroin solutions and an all-aqueous mode for preparation of concentrated aqueous fibroin solutions that avoids the use of organic solvents, direct additives, or harsh chemicals. The invention further provides for the use of these solutions in production of materials, e.g., fibers, films, foams, meshes, scaffolds and hydrogels.

Concentrated aqueous silk fibroin solution and use thereof

The present invention provides for concentrated aqueous silk fibroin solutions and an all-aqueous mode for preparation of concentrated aqueous fibroin solutions that avoids the use of organic solvents, direct additives, or harsh chemicals. The invention further provides for the use of these solutions in production of materials, e.g., fibers, films, foams, meshes, scaffolds and hydrogels.

Producing fibres by extruding onto a treatment device

The apparatus comprises a feedstock reservoir 20, a spinneret 30, an extrusion opening 40, a treatment drum 50 and a storage device 100. The drum 50 is a short distance from the opening 40. The direct physical contact between the extrudate and the surface of the treatment drum 50 enables change of chemical and physical properties of the extrudate, thereby allowing, for example, precise control of the crystallization processes of the extrudate. Preferably the reservoir 20, spinneret 30 or drum 50 is permeable. The apparatus produces mechanically strong fibres from: biopolymers especially spider or silkworm silk; semiconductors; and liquid crystals.

Apparatus and method for the manfacture of a silk mono-filament with a high tensile strength

A method and an apparatus (20) for the manufacture of a single silk mono filament (60). The single silk mono filament (60) has a tensile strength of above 40 Ncwtons. The single silk mono filament (60) has applications as a musical string or a medical device.

Method of manufacturing a biopolymer optical waveguide

A method of manufacturing a biopolymer optical waveguide includes providing a biopolymer, unwinding the biopolymer progressively to extract individual biopolymer fibers, and putting the unwound fibers under tension. The tensioned fibers are then cast in a different polymer to form a biopolymer optical waveguide that guides light due to the difference in indices of refraction between the biopolymer and the different polymer. The optical fibers may be used in biomedical applications and can be inserted in the body as transmissive media. Printing techniques may be used to manufacture the biopolymer optical waveguides.

Methods of making drawn silk fibers

The present invention relates to compositions and method for drawing egel silk fibroin fibers. The resulting fibers can transmit light and hence can be used as optical fiber. Silk fibroin fiber is produced by a method comprising applying an electric field to a solubilized silk fibroin solution to create a silk fibroin gel; converting the silk fibroin gel to a viscous silk liquid; and drawing a silk fiber from the viscous silk liquid. The silk fiber of the invention can be used in materials such as textile, medical sutures, and tissue materials, as well as conferring optical properties into these materials.

Method for producing polymer aggregate

Peptide-based nanofibrillar materials

Disclosed herein are nanofibrillar materials and aerogel-like materials comprised of nanofibrils, and methods for making such materials.

Shape memory silk materials

The present application relates to silk fibroin-based materials, methods for making and using the same. Provided materials exhibit shape memory characteristics while showing comparable or better volumetric swelling, biocompatibility and/or degradability when compared to current memory polymers derived from either natural or synthetic materials.

Biomimetic mechanical tension driven fabrication of nanofibrillar architecture

This present disclosure provides methods for utilizing such forces in when generating nanofibrillar constructs with engineered morphology from the nano- to macro-scales. Using for example, a biopolymer silk fibroin as a base material, patterns an intermediate hydrogel were generated within a deformable mold. Subsequently, mechanical tension was introduced via either hydrogel contraction or mold deformation, and finally a material is reentrapped in this transformed shape via beta-sheet crystallization and critical point drying. Topdown engineered anchorages, cables, and shapes act in concert to mediate precision changes in nanofiber alignment/orientation and a macroscale form of provided nanofibrillar structure. An ability of this technique to engineer large gradients of nano- and micro-scale order, manipulate mechanical properties (such as plasticity and thermal transport), and the in-situ generation of 2D and 3D, multi-tiered and doped, nanofibrillar constructs was demonstrated.

Silk biomaterials and methods of use thereof

The present invention provides an all-aqueous process and composition for production of silk biomaterials, e.g., fibers, films, foams and mats. In the process, at least one biocompatible polymer, such as poly(ethylene oxide) (PEO) (a well-documented biocompatible material), was blended with the silk protein prior to processing e.g., electrospinning. We discovered that this step avoids problems associated with conformational transitions of fibroin during solubilization and reprocessing from aqueous solution which lead to embrittled materials. Moreover, the process avoids the use of organic solvents that can pose problems when the processed biomaterials are exposed to cells in vitro or in vivo.

Silk fibroin materials and use thereof

The present invention provides processes for producing porous silk fibroin scaffold material. The porous silk fibroin scaffold can be used for tissue engineering. The porosity of the silk fibroin scaffolds described herein can be adjusted as to mimic the gradient of densities found in natural tissue. Accordingly, methods for engineering of 3-dimensional tissue, e.g. bone and cartilage, using the silk fibroin scaffold material are also provided.

Electrospun silk material systems for wound healing

The present invention relates to the processes of preparing silkfibroin/polyethylene oxide blended materials, and the resulting materials thereof, which are suitable for biomedical applications such as wound healing. In particular, the electrospun silk fibroin/PEO mats with a silk:PEO blend ratio of 2:1 to 4:1, treated with controlled evaporation, constraint-drying techniques, and/or alcohol treatment, and/or PEO extraction, demonstrate suitable physical and biofunctional properties, such as fiber structure, topography, absorption, water vapor transmission rates, oxygen permeation, and biodegradability, relevant to biomaterial systems with utility for wound dressings.