COMPOSITION FOR IMPROVING INTESTINAL HEALTH COMPRISING RED YEAST RICE EXTRACT

Hereinafter, the present invention is described therein that are directionally.

In one aspect, the specification disclosure to the techniques described monascus; or monascus extract composition for improving intestinal germ gun including number [...] substrate.

(Red yeast rice) is an apparatus and a fermented rice monascus germ monascus or monascus rice culture as meaning, for example general rice [...] monascus bacteria (Monascus Pilosus, MonascusPurpureus, MonascusAnka, MonascusRuber) Is 15 to 30 days during the odor of red of conservatism the features. Monascus red dye prepared by the method of cooking is famous throughout the world which is used of duck Beijing. Lowering the blood cholesterol level lowering efficacy in vivo monascus hypertension and well known. This monascus cholesterol improving efficacy of natural dye is a major cause component is red and assumes a choline K, this drug for the treatment of hyperlipidemia in the biomass in the process to produce a star [...] developed Lovastatin is famous throughout the world.

Monascus monascus itself as well as monascus manufactured goods in, e.g. drying, grinding, such as fermentation of second form including additional processing means other.

Monascus extract as well as extract betulin of monascus workpiece, e.g. at dried, concentrated, fraction, such as fermentation of second form including additional processing means other. In addition, the commercial extract monascus accordingly, or the normal extraction method are advantageously easier to use in appropriately selected, combination, can be obtained by the addition or change.

One exemplary embodiment, said monascus extract, the relayed number but not limited to, water, lower alcohols of 1 to 4 carbon atoms (e.g., methanol, ethanol, butanol or the like), ethylene, acetone, hexane (hexane), ether, chloroform, ethyl acetate, butyl acetate, dichloro methane, N, N - dimethyl formamide (DMF), methylene chloride, dimethyl sulfoxide (DMSO), glycerin, butylene glycol, propylene glycol, deep with it will bloom, [leyn the glycol, methylene chloride and [...] selected from the group consisting of be a 1 or more extraction solvent extract of white.

One exemplary embodiment, be a hydrophilic solvent extract of white said monascus extract.

One exemplary embodiment, said hydrophilic the solvent can be water, distilled water, or sterile, be positive number, phosphate buffer saline (PBS), methanol, ethanol, 1 - propanol, 2 - propanol, 1 - rearranged pentanols, 2 - butoxyethanol, ethylene writing this call, acetone and 2 - group can be selected from the non-polymerization.

One exemplary embodiment, the extract number bath method for supercritical fluid extraction, subcritical total extraction, lycopene, hot-extracted, reflux cooling extraction, using high pressure extraction or ultrasonic extraction method or like resin and method including using the party industry such as XAD and HP-a 20 using conventional extraction method can be. Specifically, heating process is performed at room temperature but can be extracted reflux extraction or reflux, the number is not one. In addition, corresponding to about 1 to 15 times building monascus extraction solvent is a solvent extraction can be applied, specifically about 10 times can be extracted by the application of solvent but, limited to are not correct. In addition, extraction recovery is 1 to 5 times may be, specifically 3 can be extracted but times, which are not limited to, extraction time is 2 to 100 may be time, specifically 10 to 70 can be a time, the one number are not disclosed.

Improving intestinal germ gun stores easily, the proliferation or growth of the recombinant expression vector of the promote proliferation or growth number intestinal beneficial intestinal feedstuff is one billion, intestinal beneficial bacteria and balancing feedstuff which means that the other.

The means by which intestinal beneficial bacteria, intestinal provided by mixing in a microorganism can be efficacy without affecting general term. For example, intestinal beneficial bacteria can be a pro bio tic [su. For example, one said intestinal beneficial bacteria the number not, a lactic acid bacterium (Bifidobacterium), Polyester (Lactobacillus), Into Streptococcus lactoperoxidase (Lactococcus), Streptococcus genus (Streptococcus), At the time of which it carries on shoulder oh as reports into (Akkermansia), Into plaque curl pak reel bud (Faecalibacterium), Or into faecalis (Enterococcus) Can be comprising.

Intestinal feedstuff is obtained, such as the middle of intestinal materials in a microorganism can be general term deleterious effects which negatively affects birds. For example, said intestinal feedstuff also relates not one number, e. coli (EscherichiaColi), Into [phwu simple reel bud (Fusobacterium), Clostridium genus (Clostridium), Into star pillow nose [khwu (Staphylococcus), Or disaccharides into (Porphyromonas) Can be comprising.

One exemplary embodiment, said composition to enhance the proliferation or growth of microbial growth or proliferation of intestinal beneficial intestinal feedstuff number can be a billion.

One exemplary embodiment, beta - billion in writing base [khwu [ni day [cu activity said composition can number. In writing base [khwu [ni day [cu - beta, intestinal feedstuff as a marker, beta - has been found that when active in writing base [khwu [ni day [cu, number of intestinal feedstuff plant billion meaning can be know.

One exemplary embodiment, said composition number billion activity can trip the toe this [cu which it sells. Trip the toe this [cu which it sells, intestinal feedstuff as a marker, trip the toe this [cu which it sells active has been found that when, these plant billion number of intestinal feedstuff can be know.

One exemplary embodiment, the content of a fatty acid can be intestinal short said composition. The specification in short chain fatty acid (Short a-chain fatty acids) RM, hereinafter means including two carbon atoms can be fatty acid 6. Said short the fatty acid acetate, propionate (propionate), isobutyrate (isobutyrate), butyrate (butyrate), selected from the group consisting of isocyanate (isovalerate) and at least one ballet [thu be a valet rate (valerate).

One exemplary embodiment, be a bowel movement said composition to improve the function. Bowel function improving the means by which, for example regularly or irregular bowel movement, constipation or diarrhea symptoms improve the can.

One exemplary embodiment, be pre-it will be a bio tic [su said composition. In another aspect, the specification is monascus; extract of microbial growth and the growth of beneficial intestinal feedstuff or monascus number side for a bio tic [su [...] billion and intestinal including number, this synergistic effect that helps enhancing the effect of pro bio tic [su flow tides. The, said free bio tic [su fibrous, oligosaccharide, and galacto oligosaccharide [...] toe raising party 1 or more can be selected from the group consisting further comprises.

Pro bio tic [su stores, in the body a healthy means affecting the microorganisms can be.

Free bio tic [su column, or promoting the growth of beneficial microorganisms including bacteria used by the microorganism by means of the host and a healthy component which allows the fiber can be.

One exemplary embodiment, said composition constipation, diarrhea, or gut for improving health food related disorders sprayable compositions disclosed.

One exemplary embodiment, said composition constipation, diarrhea, or gut for preventing or treating of diseases associated sprayable compositions disclosed.

The middle of the population reaches a large organ microorganism within the tube to be coated by intestinal germ gun balance starts to form. The individual microorganisms intestinal germ gun vitamin supply, it has infection-preventing and field function, thereby the germ collective will composition constipation, field generation of related disorders and deep relationship when it is judged that known (Mistuoka T. Bifidobacteria Microflora, 1 (1): 3, 1982).

The specification disclosure to stimulate the growth and proliferation of intestinal beneficial intestinal feedstuff compositions of microbial growth or proliferation or to improve intestinal germ gun number billion to bowel movement function, as well as acts in conjunction with pro bio tic [su field function is obtained on a synergistic effect in enhancing the effectiveness of pre-with bio tic [su pro bio tic [su flow tides. This monascus extract effect, as the major components thereof effective in improving aqueouis but very unstable monascus of cholesterol are known as having the properties of the lipophilic precipitation in K or K assumes a choline is major active components including water that is difficult to extract assumes a choline hydrophilic solvent monascus extract appears even 2000.

In another aspect, the specification disclosure to compositions including said composition for improving health field kit number [...] substrate. The, said monascus; or monascus extract composition can be administered in conjunction with including pro bio tic [su.

Said combination, monascus; extract including administering a composition including composition or monascus pro bio tic [su simultaneously, one after the other of the first or both sense including both administration are used.

The pro bio tic [su said, pro bio tic [su including a form of composition or pharmaceutical health food composition may be, for example, lactic acid bacteria including the [hyo it will be a water be induced.

One exemplary embodiment, said kit further comprising a manual, manual said is, 0 said composition. 1 To 2,000 mg/kg/day in a dosage administration including content that may be disclosed.

One exemplary embodiment, be said administration is oral administration.

The specification according to composition said composition effective pharmaceuticals suppressed compatible mineral or organic carrier applied to solid, semi-solid or liquid oral administration or parenteral administration in the form of number to number number number can be under anger.

For oral administration number number positive number as said, ring number, number granules, capsule number, acid number, number of fine-grained, component number, device for number, number syrup, pellet number or the like is cited. In addition, a number number number as said scanning for parental administration, drip number, ointment, lotion, spray, suspension number, oil number, like left number is cited. The specification hereinafter number number number number under anger according to commercial law for an effective ingredient of order number can be surfactants under anger, number excipient, colorant, flavoring agents, the preservation, stable number, buffer number, suspension number, other transmission using auxiliary number can be appropriately.

In addition, pharmaceutical compositions according to the desired method oral, parenteral, rectal, topical, transdermal, intravenous, intramuscular, intraperitoneally, such as can be avoid administration, the active ingredient of pharmaceutical compositions administered subject age, sex, weight, severity and pathologies, depending on rotor will determine routes or prescription. This factor based on the level of one skilled dose determination is composed of, for example of 1 dose 0. 1 To 2,000 mg/kg are disclosed.

The specification number number functional foods or health food composition in a variety of types of food additives [...] substrate. Specifically, said composition including leaching difference, tea, beverage, milk, cheese, yogurt, juice, health care number number locus can be working, other various food additives can be used in form of a number.

In addition, food composition without impairing the main effect within a range that the active ingredient to this synergistic effect such that main effect can further include other ingredients. For example, properties for enhanced perfume, dye, sterilization number, anti-oxidation number, number of a hydantoin, moisturizing number, number permanently, an inorganic salt, synthetic polymer substance or the like can be further comprises addition of emulsion number and number. In addition, a water soluble vitamin, utility vitamin, polymer peptide, such as seaweed extract can be further includes preparing a gel and an auxiliary component. Said components are present in the number or is a power one skilled can without difficulty selecting enemy compound, diffuses the specification can be within a range that does not impair the aim of and effect. For example, add-on greater than said components based on the total composition, 0. 01 To 5 weight %, or 0. 01 To 3% by weight may be in the range disclosed.

In another aspect, the specification disclosure to the techniques described monascus; or monascus extract to promote growth of microorganisms intestinal including intestinal microbial culture medium composition which number [...] substrate. Said intestinal microorganisms are present in the in vitro culturing can be administered to a warm-or artificially.

One exemplary embodiment, said culture medium composition that are to promote the celebration which is intestinal growth of microorganisms can be.

One exemplary embodiment, the axis which is said culture medium composition to promote other biological intestinal growth of microorganisms can be campaigns, the axis which is said other intestinal microorganisms are of a living human, simian, bovine, cattle, cow, chlorine, horse, amount, Pig, mouse, RAT, hamster, mouse, cavy, two, feline, deer, elephant, chlorine, chicken, [...], turkey, duck, ostrich, quail, camel, rabbit, fish, flatfish, rockfishes, [...], eels, tuna, prolactin, resultant, trout, direction right, loach, frog, grows up, drawer, sea cucumber, [...], squirt, Marsh snail, turban shell, mud snail, bulge, bee, grasshopper, silkworm, or nematode or present in the intestinal tracts of persons who, the same culturing in vitro can be artificially.

One exemplary embodiment, said monascus extract water, lower alcohols of 1 to 4 carbon atoms (e.g., methanol, ethanol, butanol or the like), ethylene, acetone, hexane, ether, chloroform, ethyl acetate, butyl acetate, dichloro methane, N, N - dimethyl formamide (DMF), methylene chloride, dimethyl sulfoxide (DMSO), glycerin, butylene glycol, propylene glycol, deep with it will bloom, [leyn the glycol, 1 or more solvents selected from the group consisting of methylene chloride and [...] be a extract of white.

One exemplary embodiment, be a hydrophilic solvent extract of white said monascus extract.

One exemplary embodiment, said monascus extract monascus water extract; ethanol extract; or be a mixture of water and ethanol extract.

One exemplary embodiment, said culture medium composition be a recombinant expression vector of intestinal beneficial and facile proliferation or growth.

One exemplary embodiment, the intestinal short chain fatty acid (short chain fatty acid) content of said culture medium composition culture can be increase.

Hereinafter, the present invention broadcast receiver through more detailed in the embodiment. In the embodiment for the present invention is only to exemplify these provided, in the embodiment of the present invention interprets these range number by one person with skill in the art-case is not in the in the art to will.

In the embodiment 1. Number of bath agent rice monascus

Participant are stored in appropriate size 200 g and water to rice monascus manufactured by adding 2 L reflux extraction when the extraction vessel monascus rice, filter paper after filtering extract are obtained. When the extraction process is embodiment 1 times, flecks agent after monascus rice a 7 g obtained.

On the other hand, Korean food scientific bulletin ' monascus from generating and extracting optimizing monacolin K (KOREAN J. FOOD SCI. TECHNOL. Vol. 35, No. 5, Pp. 830 - 834 (2003))' According to, monascus extract of cervi in K while having high contents of monatin choline, monascus water extract assumes a nearly not detected in K as in the picomolar choline. , the specification disclosure to the monascus extract effect on intestinal germ gun independently of monatin choline K can be know.

In the embodiment 2. Monascus rice ethanol extract number bath

Participant are stored in appropriate size and concentration to 200 g of rice monascus manufactured 2 L ethanol 95% when the reflux extraction and applying an extraction vessel rice monascus, filter paper after filtering extract are obtained. When the extraction process is embodiment 1 times, the ethanol extract 2 g after flecks monascus rice obtained.

Test example 1. In contact with human fecal sample

For 3 months in antibiotic prescription number 7 has not yet been subjected to intestinal microbiological culture name is from 10 - 30 g of fecal sample to ensure health Korean Young people have, 30 minutes mixing same transferred anaerobic work is positioned on me. Number added to 40 ml glycerol is carried out after cryopreservation for storing into one which is included in the refrigerant blend free [...] have, intestinal culturing microorganisms during the same for inoculation sample at night.

Test example 2. Continuous anaerobic culture intestinal microorganisms

Intestinal microbial culture in a contact can be maintained constant and continuously using conducting bioreactor for anaerobic culture. Table 1 basic medium usable in culturing medium and high protein intake group such as order of media as well as simulating table 2.

Each filled with 300 ml of media kept anaerobic bioreactor gas injection after test example 1 of nitrogen after sterilization through a predetermined sample was the seeded intestinal microorganisms. 24 Hours performing batch culture of media using continuous culture medium performing shows when the total dwell time 24 time has been maintained. Culture was maintained temperature 37 °C, pH is 1 N sodium hydroxide 1 N hydrochloric acid solution using a solution 5. 5 Been maintained. Culture solution is recovered by sample 1 times daily for confirming the own total 30 or more short-chain fatty acid (short chain fatty acid, SCFA) analysis that has been culture homestretch.

Test example 3. Intestinal germ gunThe wetness sensor function improving efficacy/search

Candidate material for improving the intestinal flora and rapidly evaluate efficacy by using biotechnological conducting 96 well plate. The 10 μl into a continuous anaerobic culture over a microorganism culture medium was seeded onto a 1 ml for searching a table such as the composition of the intestinal 3. The intestinal flora improving agent concentration of 100 micro g/mL and monascus ethanol extract assessing the efficacy for monascus (0. 2% DMSO) was treated with 48 hours at a temperature of 37 °C engineers. The positive controls with it cuts intestinal germ gun is well known that a representative [...] prefetches processed fructo-oligosaccharide (Fructooligosaccharides, FOS, 1 mg/mL) O2, while at the same time known as the antibiotic chloramphenicol in the damaged intestinal germ gun number (10 or 40 micro g/mL) that compares the effect of improving intestinal germ gun using his. After using a microorganism culture, degree of intestinal microbial growth, feedstuff assay, short chain fatty acid content was improved by a reduction in intestinal germ gun verify efficacy.

Test example 3 - 1. If the intestinal microbial growth

Fructo-oligosaccharide (FOS), antibiotic number, monascus agent, monascus ethanol extract by 96 well plate 100 μl culture processed then transferred, 600 nm absorbance using a relative degree of microbial growth Plate reader groups were measured.

As a result, monascus agent, monascus beverage exhibits high intestinal bacteria growth in the first put has been confirmed. Specifically, basing the pre-processed fructo-oligosaccharide (FOS) intestinal germ gun is demonstrated an improvement effect of a recombinant expression vector of intestinal growth while [...] height, at the time of administration of the antibiotics are known to damaged intestinal germ gun number (chloramphenicol) is also used for capable of intestinal bacteria growth is lowered. Monascus agent, to form an expression vector of the monascus extract intestinal germ gun has been enhanced intestinal is beverage exhibits high monascus improvements confirm growth were. The intestinal germ gun growth-promoting effect normal medium conditions (also 1) general monascus extract as well as high protein intake has been confirmed that the culture conditions simulating those similarly even (2 also).

Test example 3 - 2. Intestinal microorganism culture of β-a glucuronidase activity monitoring

Fructo-oligosaccharide (FOS), antibiotic number, monascus agent, monascus extract is treated using microorganism culture in writing base [khwu [ni day [cu - beta rays were measured activity. In writing base [khwu [ni day [cu - beta activity monitoring may be used standard test item to verify that the health efficacy are disclosed. The higher the return electrode has a high distribution of feedstuff in writing base [khwu [ni day [cu beta - active in intestinal germ gun and, beta - active in distribution of feedstuff in writing base [khwu [ni day [cu low ratio low intestinal germ gun can comprise means other. Intestinal microorganism culture with the culture conditions (fermentor) anaerobically culturing solution ladles and it carries on shoulder, homestretch. 1 Anaerobic culture solution. The fructo-oligosaccharide (FOS) 0 ml, antibiotic number, monascus agent, each handling a microorganism culture was inoculated with 10 μl monascus ethanol extract after intestinal. In a sample 48 37 °C aerobically culturing enzyme solution was used to time after extraction. 96 Well plate 20 mm 4 a-nitrophenyl a-β-a D-a glucuronide 20 μl is added, with respect to the enzyme solution obtained by adding 80 μl reaction in 60 minutes 37 °C. 0. 5 N NaOH 100 μl reaction in 10 minutes after applying terminate when the centrifugal separator 3500 rpm, supernatant in absorbance 405 nm takes a long time. In order to correct for intestinal bacteria growth degree enzymatic activity after intestinal growth of microorganisms degree (OD₆₀₀ ) Value of dividing the puzzle with [khwu (%) beta - calculating the final relative [...] activity was.

As a result, basing the pre-[...] fructo-oligosaccharide (FOS) processed intestinal germ gun improvement effect is demonstrated in writing base [khwu [ni day [cu - beta activity while lowering, the intestinal bacteria that are known to antibiotics (chloramphenicol) intestinal germ gun damaged number is making sure that the capable of increasing activity in writing base [khwu [ni day [cu - beta in a culture. Monascus agent, upon administering an active beta - type plastic the lower beverage exhibits high monascus in writing base [khwu [ni day [cu corn stalk and a feedstuff distribution ratio mode of the beneficial bacteria distribution ratio monascus extract is present in the intestinal germ gun confirm that a first call request (3 also).

Test example 3 - 3. Intestinal microorganism culture of tryptophanase activity monitoring

Fructo-oligosaccharide (FOS), antibiotic number, monascus agent, monascus extract is treated intestinal microorganism culture were measured activity using trip the toe this [cu which it sells. Trip the toe this [cu which it sells health efficacy assay may be used to verify that the item standard test are disclosed. The higher the return electrode has a high active intestinal germ gun and distribution of feedstuff in the toe this [cu which it sells trip, trip the toe this [cu which it sells active means can comprise low ratio low intestinal germ gun distribution of feedstuff in 2000. Intestinal microorganism culture with the culture conditions (fermentor) anaerobically culturing solution ladles and it carries on shoulder, homestretch. 1 Anaerobic culture solution. 0 Ml was inoculated with 10 μl sample after processing the natural intestinal microorganism culture. In anaerobic incubator 48 37 °C culture nor post-enzyme solution was used to sample time. Prydoxal phosphate 2. 75 Mg, disodium EDTA dehydrate 19. 6 Mg, a bovine serum albumin 10 mg 0. 05 M potassium phosphate buffer (pH 7. 5) Number was reactive mixed solution for 1 to 100 ml high pressure liquid coolant. 96 Well plate 40 μl reaction mixed solution on 20 μl culture sample, a reaction with respect to the mixed and are in 40 μl 40 mm tryptophan 37 °C 60 minutes. Reagents (p provided demethylaminobenzaldehyde 14. 7 G, 948 ml 95% ethanol, H2SO 4 52 ml) then terminate reaction by adding 100 μl, 3500 rpm when the supernatant were measured absorbance 550 nm in 10 minutes centrifuging takes in. In order to correct for intestinal bacteria growth degree enzymatic activity after intestinal growth of microorganisms degree (OD₆₀₀ ) Value of dividing the final relative trip the toe this [cu which it sells his calculating active (%).

As a result, basing the pre-[...] fructo-oligosaccharide (FOS) effect is demonstrated intestinal germ gun processed one high lowering activity while the toe this [cu which it sells, the intestinal bacteria that are known to antibiotics (chloramphenicol) number is damaged intestinal germ gun trip in a culture of making sure that the capable of increasing activity the toe this [cu which it sells. Monascus agent, monascus ethanol extract upon administering an active low trip the toe this [cu which it sells a rubber-type plastic, the beneficial bacteria distribution ratio distribution ratio mode of the corn stalk and a feedstuff monascus extract is present in the intestinal germ gun confirm that a first call request (4 also).

Test example 3 - 4. Short chain fatty acid content analysis

Fructo-oligosaccharide (FOS), antibiotic number, monascus agent, monascus beverage exhibits high content of short-chain fatty acid (short chain fatty acid, SCFA) processed in intestinal microorganism culture for analysis, gas chromatography (GC) analysis was embodiment. Analyzing a test sample is 10 minutes in 96 well plate 400 μl 50% sulfuric acid (sulfuric acid) to 3500 rpm centrifuging dehydrates obtained after 20 μl through the stabilizing, 40 μl (internal standard, 1% 2 a-methyl pentanic acid) is the standard adding 400 μl (ethyl ether) about 2 minutes this is selected ethyl ether is mixed and pulverized (vortex) at both ends. After the separation of the sample and the dirt collecting chamber in the upper layer by recovering the ethyl ether was embodiment GC analysis. Bruker 450 a-GC model and analysis device is used, the columns Supelco yarn Nukol Fused silica capillary column (30 mm × 0. 25 Mm × 0. 25 Micro m) O2, table 4 analysis conditions such as disclosed.

As a result, short-chain fatty acid content in regulated nothing control 18. 6 Mm has been measured. While, basing the pre-processed [...] fructo-oligosaccharide (FOS) processing is demonstrated an improvement effect of intestinal germ gun [...] content 25. Has been increased to 41 mm, a short-chain fatty acids that are known to antibiotic administration number intestinal germ gun damaged (chloramphenicol) content of 2. 03 Mm is also used for lower backed up in the queue. Monascus agent, the content of short-chain fatty acids to form monascus beverage exhibits high 20. 56 And 20. 11 Mm is also used for high to first call request. The monascus (table 5) were confirm that a germ gun intestinal extract.

The example described below according to one aspect of the specification number number composition but, various approaches and other applicable even number type, the present invention is a bending not only specifically described defining a bending disclosed.

Example number number 1. Also number

Monascus extract 150 mg, palm oil 2 mg, 8 mg stiffening type selling, mixing and lecithin hwang lead 4 mg 6 mg, 400 mg per capsule 1 according to a conventional method by number number was also filled with high pressure liquid coolant.

Example number number 2. Positive number

Monascus extract 150 mg, 100 mg glucose, red Ginseng extract 50 mg, 96 mg and magnesium stearate 4 mg 40 mg ethanol mixing adding 30% starch granules is formed, and a lubricant homogeneously in a one or two-gate 60 °C to a positive number.

Example number number 3. Number granules

Monascus extract 150 mg, 100 mg glucose, 30% ethanol added 600 mg 50 mg 100 mg Ginseng extract and starch granules mixed formed, 60 °C added in granules filling-gate formed fabrics. 1 G final weight contents of electrodes respectively.

Example number number 4. Number drink

150 Mg monascus extract, 10 g glucose, 50 mg Ginseng extract, 2 g citric acid be positive and number 187. 8 G-gate mixing bottle filling. Final contents is set to 200 ml capacity.

Example number number 5. Number tank health food

Monascus extract............... 1000 Mg

Vitamin mixtures

Vitamin A acetate............ 70 Micro g

Vitamin E................. 1. 0 Mg

Vitamin B1................ 0. 13 Mg

Vitamin B2................ 0. 15 Mg

Vitamin B6................ 0. 5 Mg

Vitamin B12................ 0. 2 Micro g

Vitamin C.................. 10 Mg

Biotin................... 10 Micro g

Nicotinic acid amide............... 1. 7 Mg

Folic acid.................... 50 Micro g

Pantothenic acid calcium............... 0. 5 Mg

Inorganic mixture

Number 1 sulfate iron................. 1. 75 Mg

Zinc oxide.................. 0. 82 Mg

Magnesium carbonate................ 25. 3 Mg

Number 1 potassium phosphate................ 15 Mg

Number 2 calcium phosphate................ 55 Mg

Citric acid potassium................. 90 Mg

Calcium carbonate................. 100 Mg

Magnesium chloride................ 24. 8 Mg

The ratio of mixture of vitamins and minerals said preferred embodiment suitable component mixed but relatively health food color, its combined arbitrarily modified embodiment and reflecting element, said manufacturing method of high pressure liquid coolant according to conventional health food components may be mixed together and then granules number, number of health food composition according to conventional method can be use of the bath.

Example number number 6. Number of health beverage tank

Monascus extract............... 1000 Mg

Citric acid.................. 1000 Mg

Oligosaccharide................. 100 G

Japanese apricot concentrate................. 2 G

Taurine................... 1 G

Total number applied positive number........... 900 Ml

About 1 time of said conventional health drink manufacturing method according components may be mixed together and then heated with agitation 85 °C, made sterile by filtration after sealing sterilizing solution was refrigerated container 2 l acquires. Then, the health drink composition number bath as well as conventional method.

Said composition ratio suitable component but a preferred number number displayed relatively infused example mixed layer or demand, demand country, use in applications such as use at least in certain regions, and optionally modified embodiment in accordance with the National broadcast blended in such a proportion reflecting element disclosed. In the present invention if said person with skill in the art based on the content of the present invention is provided in a variety of different applications and deformation will performing category.

Or more, the present invention techniques that are directionally-specific content portion shaped conductor, to the party industry in person with skill in the art, such traded only in a preferred embodiment solar day is received, the range of the present invention apparently is the number that are not one will. The substantial range of the present invention defined by appended claim and their equivalent that will.