PROCESS FOR PRODUCING ALCOHOLS UNDER AEROBIC CONDITIONS AND PRODUCT EXTRACTION USING OLEYL ALCOHOL
The present invention relates to a biotechnological method of producing alcohols including higher alcohols from a carbon source in aerobic conditions. In particular, the method relates to a biotechnological production of at least one alcohol in an aqueous solution in the presence of oxygen and a means of extracting the alcohol from the aqueous solution. Biotechnological methods of producing alcohols, particularly ethanol are well known in the art. Especially the use of acetogenic bacteria on various carbon sources to produce ethanol and/or acetate is well known. However, in most cases, the production of alcohols can only be successfully carried out in the absence of oxygen. This phenomenon is confirmed at least by Brioukhanov, 2006, Imlay, 2006, Lan, 2013 and the like where it is shown that acetogenic bacteria do not successfully produce ethanol in aerobic conditions. Therefore, in the current methods known in the art, carbon substrates comprising oxygen, such as waste gases from steel mills are first processed to remove the oxygen before they are introduced to the acetogenic cells for ethanol and/or acetate production. The oxygen separation step makes the process more expensive and time consuming. Further, there may be some loss in the raw materials during this step of separation. There is thus a need in the art for a means of producing ethanol and/or acetate in the presence of oxygen. Ethanol may then be used as a raw material for production of higher carbon compounds such as alcohols, acids and the like. For example, butanol and higher alcohols have several uses including being used as fuel. For example, butanol in the future can replace gasoline as the energy contents of the two fuels are nearly the same. Further, butanol has several other superior properties as an alternative fuel when compared to ethanol. These include butanol having higher energy content, butanol being less “evaporative” than ethanol or gasoline and butanol being easily transportable compared to ethanol. For these reasons and more, there is already an existing potential market for butanol and/or related higher alcohols. Butanol and other higher alcohols are also used as industrial solvents. Similarly, propanol is a solvent used in the pharmaceutical industry for resins and cellulose esters amongst other compounds. This solvent, which is better known as isopropanol or isopropyl alcohol, is widely used on printing ink and in the printing industry. 1-propanol is produced in nature by the decomposition of organic materials by a variety of microorganisms and may be found in plants and fusel oil. 1-propanol can also be produced from petrochemically-derived ethene by a reaction with carbon monoxide and hydrogen to give propionaldehyde, which is then hydrogenated. It is also a by-product of methanol manufacture and may be produced from propane directly or from acrolein. Propanol has further other potential uses. All these alcohols are currently being produced by cracking gasoline or petroleum which is bad for the environment. Further, most of these alcohols produced commercially are being extracted by conventional procedures such as distillation. Distillation of alcohol using these conventional means may require more input of energy than the output energy capability of the extracted alcohol. Accordingly, there is a need for an environmentally friendly and bio-based efficient alternative to the petro-based production process of alcohols and an energy-efficient way of extracting these bio-based alcohols. The present invention provides a biotechnological means of producing at least one alcohol from a carbon source in aerobic conditions. The carbon source may comprise carbon dioxide and/or carbon monoxide. In particular, the method comprises at least two parts. One part that involves the formation of the alcohol in an aqueous medium from the carbon source and a further part which involves the extraction of the alcohol from the aqueous medium. In one aspect of the present invention, there is provided a method of producing at least one alcohol from a carbon source, the method comprising:
The method according to any aspect of the present invention may be an aerobic method of producing at least one isolated alcohol from a carbon source. An isolated alcohol may refer to at least one alcohol that may be separated from the medium where the alcohol has been produced. In one example, the alcohol may be produced in an aqueous medium (e.g. fermentation medium where the alcohol is produced by specific cells from a carbon source). The isolated alcohol may refer to the alcohol extracted from the aqueous medium. In particular, the extracting step allows for the separation of excess water from the aqueous medium thus resulting in a formation of a mixture containing the extracted alcohol. The extracting medium may also be referred to as the ‘extraction medium’. The extraction medium may be used for extracting/isolating the alcohol produced according to any method of the present invention from the aqueous medium wherein the alcohol was originally produced. At the end of the extracted step, excess water from the aqueous medium may be removed thus resulting in the extracting medium containing the extracted alcohol. The extracting medium may comprise a combination of compounds that may result in an efficient means of extracting the alcohol from the aqueous medium. In particular, the extracting medium may comprise: (i) at least one hydrocarbon comprising at least 5-18 carbon atoms, and (ii) at least one polyoxyalkylene polymer. The extraction medium according to any aspect of the present invention may efficiently extract the fermentation alcohol into the hydrocarbon-polyoxyalkylene polymer extracting medium. In particular, the hydrocarbon in the mixture comprising hydrocarbon-polyoxyalkylene polymer may be least one alkane. More in particular, the alkane may comprise at least 5 carbon atoms. This extracting medium of a mixture of polypropylene glycol and at least one alkane may be considered suitable in the method according to any aspect of the present invention as the mixture works efficiently in extracting the desired alcohol in the presence of oxygen. In particular, the mixture of polypropylene glycol and at least one alkane may be considered to work better than oleyl alcohol as an extracting medium as the double bond in oleyl alcohol may be considered to be sensitive to the presence of oxygen. Even more in particular, the alkane may comprise at least 5 to 18 carbon atoms. In one example, the alkane may be selected from the group consisting of pentane, hexane, heptane, octane, nonane, decane, undecane, dodecane, tridecane, tetradecane, pentadecane, hexadecane, heptadecane and octadecane. In a further example, the extracting medium may comprise a mixture of polypropylene glycol and hexadecane. More in particular, the mixture of polypropylene glycol and hexadecane may comprise about 50, 55, 60, 65, 70, 75, 80% w/w of polypropylene glycol and 50, 45, 40, 35, 30, 25, 20% w/w hexadecane. Even more in particular, the mixture of polypropylene glycol and hexadecane may comprise 70% w/w polypropylene glycol and about 30% w/w hexadecane. In step (bi) according to any aspect of the present invention, the alcohol in the aqueous medium may contact the extracting medium for a time sufficient to extract the alcohol from the aqueous medium into the extracting medium. A skilled person may be capable of determining the amount of time needed for substantially all the alcohol to be extracted into the extracting medium. The time needed may be about, 1, 2, 3, 4, 5 6, 7, 8, 9 or 10 minutes. In some examples the time needed may be dependent on the amount of alcohol that may be extracted. In particular, the time needed to extract the alcohol from the aqueous medium into the extracting medium may be about 3 minutes. The ratio of the extracting medium used to the amount of alcohol to be extracted may vary depending on how quick the extraction is to be carried out. In one example, the amount of extracting medium is equal to the amount of aqueous medium comprising the alcohol. After the step of contacting the extracting medium with the aqueous medium, the two phases (aqueous and organic) are separated using any means known in the art. In one example, the two phases may be separated using a separation funnel. In one example, wherein the alcohol may be propanol, step (a) comprises
In another example, the alcohol may be at least one higher alcohol and step (a) comprises
The third microorganism capable of converting acetate and/or ethanol to propanol and propionic acid may refer to any microorganism that may be able to carry out fermentative production of propanol. This third microorganism may be a propionogen. Propionogens are C3-producing microorganisms. In particular, propionogens refers to any microorganism which may be capable of converting syngas intermediates, such as ethanol and acetate, to propionic acid and propanol. The terms “propionogen” or “C3-producing microorganism” refers to microorganisms which, when contacted with a substrate, convert the substrate to propanol. These microorganisms may produce the appropriate enzymes intracellularly and/or extracellularly. These propanol producing microorganisms may be capable of utilising starting material for propanol fermentation that may be waste materials. For instance, ethanol and/or acetate derived from syngas may be utilized for the propanol production. This is particularly advantageous as inexpensive starting materials can be utilized that would originally have been considered waste. This also enables the removal of waste which consequently reduces environmental pollution. In one example, the propionogen according to any aspect of the present invention may use at least the methylmalonyl-succinate pathway or the lactate-acrylate pathway to produce propanol from acetate and/or ethanol. In particular, the propionogen used according to any aspect of the present invention may be selected from the group consisting of In one example, the third microorganism may be any eukaryotic or prokaryotic microorganism that may be genetically modified. More in particular, the third microorganism may be a strain selected from the group consisting of In one example, the ethanol and/or acetate may be converted to the corresponding higher acid in the presence of the fourth microorganism capable of carrying out the ethanol-carboxylate fermentation pathway. The ethanol-carboxylate fermentation pathway is described in detail at least in Seedorf, H., et al., 2008. In particular, the fourth organism may be selected from the group consisting of In another example, the fourth microorganism may be a wild type organism that expresses at least one enzyme selected from the group consisting of E4to E14, wherein E4is an alcohol dehydrogenase (adh), E5is an acetaldehyde dehydrogenase (ald), E6is an acetoacetyl-CoA thiolase (thl), E7is a 3-hydroxybutyryl-CoA dehydrogenase (hbd), E8is a 3-hydroxybutyryl-CoA dehydratase (crt), E9is a butyryl-CoA dehydrogenase (bcd), E10is an electron transfer flavoprotein subunit (etf), E11is a coenzyme A transferase (cat), E12is an acetate kinase (ack), E13is phosphotransacetylase (pta) and E14is a transhydrogenase. In particular, the wild type fourth microorganism according to any aspect of the present invention may express at least E5, E6and E7. Even more in particular, the wild type fourth microorganism according to any aspect of the present invention may express at least E7. In another example, the fourth microorganism according to any aspect of the present invention may be a genetically modified organism that has increased expression relative to the wild type microorganism of at least one enzyme selected E4to E14wherein E4is an alcohol dehydrogenase (adh), E5is an acetaldehyde dehydrogenase (ald), E6is an acetoacetyl-CoA thiolase (thl), E7is a 3-hydroxybutyryl-CoA dehydrogenase (hbd), E8is a 3-hydroxybutyryl-CoA dehydratase (crt), E9is a butyryl-CoA dehydrogenase (bcd), E10is an electron transfer flavoprotein subunit (etf), is a coenzyme A transferase (cat), E12is an acetate kinase (ack) E13is phosphotransacetylase (pta) and E14is a transhydrogenase. In particular, the genetically modified fourth microorganism according to any aspect of the present invention may express at least enzymes E5, E6and E7. Even more in particular, the genetically modified fourth microorganism according to any aspect of the present invention may express at least E7. The enzymes E4to E14may be isolated from The term “acetate” as used herein, refers to both acetic acid and salts thereof, which results inevitably, because as known in the art, since the microorganisms work in an aqueous environment, and there is always a balance between salt and acid present. In particular, the second acetogenic microorganism in a post exponential phase may be in the stationary phase of the cell. The acetogenic cells in the log phase allow for any other acetogenic cells in the aqueous medium to produce acetate and/or ethanol in the presence of oxygen. The concentration of acetogenic cells in the log phase may be maintained in the reaction mixture. Therefore, at any point in time in the reaction, the reaction mixture comprises acetogenic cells in the log phase and acetogenic cells in another growth phase, for example in the stationary phase. A skilled person would understand the different growth phases of microorganisms and the methods to measure them and identify them. In particular, most microorganisms in batch culture, may be found in at least four different growth phases; namely they are: lag phase (A), log phase or exponential phase (B), stationary phase (C), and death phase (D). The log phase may be further divided into the early log phase and mid to late log/exponential phase. The stationary phase may also be further distinguished into the early stationary phase and the stationary phase. For example, Cotter, J. L., 2009, Najafpour. G., 2006, Younesi, H., 2005, and Köpke, M., 2009 disclose different growth phases of acetogenic bacteria. In particular, the growth phase of cells may be measured using methods taught at least in Shuler ML, 1992 and Fuchs G., 2007. The lag phase is the phase immediately after inoculation of the cells into a fresh medium, the population remains temporarily unchanged. Although there is no apparent cell division occurring, the cells may be growing in volume or mass, synthesizing enzymes, proteins, RNA, etc., and increasing in metabolic activity. The length of the lag phase may be dependent on a wide variety of factors including the size of the inoculum; time necessary to recover from physical damage or shock in the transfer; time required for synthesis of essential coenzymes or division factors; and time required for synthesis of new (inducible) enzymes that are necessary to metabolize the substrates present in the medium. The exponential (log) phase of growth is a pattern of balanced growth wherein all the cells are dividing regularly by binary fission, and are growing by geometric progression. The cells divide at a constant rate depending upon the composition of the growth medium and the conditions of incubation. The rate of exponential growth of a bacterial culture is expressed as generation time, also the doubling time of the bacterial population. Generation time (G) is defined as the time (t) per generation (n=number of generations). Hence, G=t/n is the equation from which calculations of generation time derive. The exponential phase may be divided into the (i) early log phase and (ii) mid to late log/exponential phase. A skilled person may easily identify when a microorganism, particularly an acetogenic bacteria, enters the log phase. For example, the method of calculating the growth rate of acetogenic bacteria to determine if they are in the log phase may be done using the method taught at least in Henstra A. M., 2007. In particular, the microorganism in the exponential growth phase according to any aspect of the present invention may include cells in the early log phase and mid to late log/exponential phase. The stationary phase is the phase where exponential growth ends as exponential growth cannot be continued forever in a batch culture (e.g. a closed system such as a test tube or flask). Population growth is limited by one of three factors: 1. exhaustion of available nutrients; 2. accumulation of inhibitory metabolites or end products; 3. exhaustion of space, in this case called a lack of “biological space”. During the stationary phase, if viable cells are being counted, it cannot be determined whether some cells are dying and an equal number of cells are dividing, or the population of cells has simply stopped growing and dividing. The stationary phase, like the lag phase, is not necessarily a period of quiescence. Bacteria that produce secondary metabolites, such as antibiotics, do so during the stationary phase of the growth cycle (Secondary metabolites are defined as metabolites produced after the active stage of growth). The death phase follows the stationary phase. During the death phase, the number of viable cells decreases geometrically (exponentially), essentially the reverse of growth during the log phase. In one example, where O2is present in the reaction mixture according to any aspect of the present invention, the first acetogenic bacteria may be in an exponential growth phase and the other acetogenic bacteria may be in any other growth phase in the lifecycle of an acetogenic microorganism. In particular, according to any aspect of the present invention, the acetogenic bacteria in the reaction mixture may comprise one acetogenic bacteria in an exponential growth phase and another in the stationary phase. In the presence of oxygen, without the presence of the acetogenic bacteria in an exponential growth, the acetogenic bacteria in the stationary phase may not be capable of producing acetate and/or ethanol. This phenomenon is confirmed at least by Brioukhanov, 2006, Imlay, 2006, Lan, 2013 and the like. The inventors thus surprisingly found that in the presence of acetogenic bacteria in an exponential growth, the acetogenic bacteria in any growth phase may aerobically respire and produce acetate and/or ethanol at more than or equal to the amounts produced when the reaction mixture was absent of oxygen. In one example, the acetogenic bacteria in the exponential growth phase may be capable of removing the free oxygen from the reaction mixture, providing a suitable environment (with no free oxygen) for the acetogenic bacteria in any growth phase to metabolise the carbon substrate to produce acetate and/or ethanol. In another example, the aqueous medium may already comprise acetogenic bacteria in any growth phase, particularly in the stationary phase, in the presence of a carbon source. In this example, there may be oxygen present in the carbon source supplied to the aqueous medium or in the aqueous medium itself. In the presence of oxygen, the acetogenic bacteria may be inactive and not produce acetate and/or ethanol prior to the addition of the acetogenic bacteria in the exponential growth phase. In this very example, the acetogenic bacteria in the exponential growth phase may be added to the aqueous medium. The inactive acetogenic bacteria already found in the aqueous medium may then be activated and may start producing acetate and/or ethanol. In a further example, the acetogenic bacteria in any growth phase may be first mixed with the acetogenic bacteria in the exponential growth phase and then the carbon source and/or oxygen added. According to any aspect of the present invention, a microorganism in the exponential growth phase grown in the presence of oxygen may result in the microorganism gaining an adaptation to grow and metabolise in the presence of oxygen. In particular, the microorganism may be capable of removing the oxygen from the environment surrounding the microorganism. This newly acquired adaptation allows for the acetogenic bacteria in the exponential growth phase to rid the environment of oxygen and therefore produce acetate and ethanol from the carbon source. In particular, the acetogenic bacteria with the newly acquired adaptation allows for the bacteria to convert the carbon source to acetate and/or ethanol. In one example, the acetogenic bacteria in the reaction mixture according to any aspect of the present impression may comprise a combination of cells: cells in the log phase and cells in the stationary phase. In the method according to any aspect of the present invention the acetogenic cells in the log phase may comprise a growing rate selected from the group consisting of 0.01 to 2 h−1, 0.01 to 1 h−1, 0.05 to 1 h−1, 0.05 to 2 h−10.05 to 0.5 Wand the like. In one example, the OD600of the cells of the log phase acetogenic cells in the reaction mixture may be selected from the range consisting of 0.001 to 2, 0.01 to 2, 0.1 to 1, 0.1 to 0.5 and the like. A skilled person would be able to use any method known in the art to measure the OD600and determine the growth rate of the cells in the reaction mixture and/or to be added in the reaction mixture. For example, Koch (1994) may be used. In particular, bacterial growth can be determined and monitored using different methods. One of the most common is a turbidity measurement, which relies upon the optical density (OD) of bacteria in suspension and uses a spectrophotometer. The OD may be measured at 600 nm using a UV spectrometer. In order to maintain the concentration of the first and second acetogenic bacteria in the reaction mixture, a skilled person may be capable of extracting a sample at fixed time points to measure the OD600, pH, concentration of oxygen and concentration of ethanol and/or higher alcohols formed. The skilled person would then be able to add the necessary component(s) to maintain the concentration of first and second acetogenic bacteria in the reaction mixture and to ensure an optimum environment is maintained for the production of ethanol and/or acetate. The term “acetogenic bacteria” as used herein refers to a microorganism which is able to perform the Wood-Ljungdahl pathway and thus is able to convert CO, CO2and/or hydrogen to acetate. These microorganisms include microorganisms which in their wild-type form do not have a Wood-Ljungdahl pathway, but have acquired this trait as a result of genetic modification. Such microorganisms include but are not limited to In particular, the acetogenic bacteria may be selected from the group consisting of Another particularly suitable bacterium may be In the reaction mixture according to any aspect of the present invention, there may be oxygen present (i.e. aerobic conditions are used). It is advantageous to incorporate O2in the reaction mixture and/or gas flow being supplied to the reaction mixture as most waste gases including synthesis gas comprises oxygen in small or large amounts. It is difficult and costly to remove this oxygen prior to using synthesis gas as a carbon source for production of higher alcohols. The method according to any aspect of the present invention allows the production of at least one higher alcohol without the need to first remove any trace of oxygen from the carbon source. This allows for time and money to be saved. More in particular, the O2concentration in the gas flow may be may be present at less than 1% by volume of the total amount of gas in the gas flow. In particular, the oxygen may be present at a concentration range of 0.000005 to 2% by volume, at a range of 0.00005 to 2% by volume, 0.0005 to 2% by volume, 0.005 to 2% by volume, 0.05 to 2% by volume, 0.00005 to 1.5% by volume, 0.0005 to 1.5% by volume, 0.005 to 1.5% by volume, 0.05 to 1.5% by volume, 0.5 to 1.5% by volume, 0.00005 to 1% by volume, 0.0005 to 1% by volume, 0.005 to 1% by volume, 0.05 to 1% by volume, 0.5 to 1% by volume, 0.55 to 1% by volume, 0.60 to 1% by volume, particularly at a range of 0.60 to 1.5%, 0.65 to 1%, and 0.70 to 1% by volume in the gas phase of the gas flow and/or in the medium. In particular, the acetogenic microorganism is particularly suitable when the proportion of O2in the gas phase/flow is about 0.00005, 0.0005, 0.005, 0.05, 0.5, 0.6, 0.7, 0.8, 0.9, 1, 1.5, 2% by volume in relation to the volume of the gas in the gas flow. A skilled person would be able to use any one of the methods known in the art to measure the volume concentration of oxygen in the gas flow. In particular, the volume of oxygen may be measured using any method known in the art. In one example, a gas phase concentration of oxygen may be measured by a trace oxygen dipping probe from PreSens Precision Sensing GmbH. Oxygen concentration may be measured by fluorescence quenching, where the degree of quenching correlates to the partial pressure of oxygen in the gas phase. Even more in particular, the first and second microorganisms according to any aspect of the present invention are capable of working optimally in the aqueous medium when the oxygen is supplied by a gas flow with concentration of oxygen of less than 1% by volume of the total gas, in about 0.015% by volume of the total volume of gas in the gas flow supplied to the reaction mixture. According to any aspect of the present invention, the aerobic conditions in which the carbon source is converted to ethanol and/or acetate in the reaction mixture refers to gas surrounding the reaction mixture. The gas may comprise at least 1% by volume of the total gas of oxygen and other gases including carbon sources such as CO, CO2and the like. The aqueous medium according to any aspect of the present invention may comprise oxygen. The oxygen may be dissolved in the medium by any means known in the art. In particular, the oxygen may be present at 0.5 mg/L in the absence of cells. In particular, the dissolved concentration of free oxygen in the aqueous medium may at least be 0.01 mg/L. In another example, the dissolved oxygen may be about 0.01, 0.02, 0.03, 0.04, 0.05, 0.1, 0.2, 0.3, 0.4, 0.5 mg/L. In particular, the dissolved oxygen concentration may be 0.01-0.5 mg/L, 0.01-0.4 mg/L, 0.01-0.3 mg/L, 0.01-0.1 mg/L. In particular, the oxygen may be provided to the aqueous medium in a continuous gas flow. More in particular, the aqueous medium may comprise oxygen and a carbon source comprising CO and/or CO2. More in particular, the oxygen and a carbon source comprising CO and/or CO2is provided to the aqueous medium in a continuous gas flow. Even more in particular, the continuous gas flow comprises synthesis gas and oxygen. In one example, both gases are part of the same flow/stream. In another example, each gas is a separate flow/stream provided to the aqueous medium. These gases may be divided for example using separate nozzles that open up into the aqueous medium, frits, membranes within the pipe supplying the gas into the aqueous medium and the like. The oxygen may be free oxygen. According to any aspect of the present invention, ‘a reaction mixture comprising free oxygen’ refers to the reaction mixture comprising elemental oxygen in the form of O2. The O2may be dissolved oxygen in the reaction mixture. In particular, the dissolved oxygen may be in the concentration of 5 ppm (0.000005% vol; 5×10−6). A skilled person may be capable of using any method known in the art to measure the concentration of dissolved oxygen. In one example, the dissolved oxygen may be measured by Oxygen Dipping Probes (Type PSt6 from PreSens Precision Sensing GmbH, Regensburg, Germany. Step (aii) of the method according to any aspect of the present invention involves contacting the acetate and/or ethanol from step (ai) with a third microorganism capable of converting the acetate and/or ethanol to propanol. In particular, the third microorganism may be genetically modified to comprise increased expression relative to the wild type cell of enzymes necessary to carry out the methylmalonyl-succinate pathway or the lactate-acrylate pathway to produce propanol from acetate and/or ethanol. According to any aspect of the present invention, the first, second, third and/or fourth microorganism may be a genetically modified microorganism. The genetically modified cell or microorganism may be genetically different from the wild type cell or microorganism. The genetic difference between the genetically modified microorganism according to any aspect of the present invention and the wild type microorganism may be in the presence of a complete gene, amino acid, nucleotide etc. in the genetically modified microorganism that may be absent in the wild type microorganism. In one example, the genetically modified microorganism according to any aspect of the present invention may comprise enzymes that enable the microorganism to produce propanol and/or propionic acid. The wild type microorganism relative to the genetically modified microorganism according to any aspect of the present invention may have none or no detectable activity of the enzymes that enable the genetically modified microorganism to produce propanol and/or propionic acid. As used herein, the term ‘genetically modified microorganism’ may be used interchangeably with the term ‘genetically modified cell’. The genetic modification according to any aspect of the present invention may be carried out on the cell of the microorganism. The phrase “wild type” as used herein in conjunction with a cell or microorganism may denote a cell with a genome make-up that is in a form as seen naturally in the wild. The term may be applicable for both the whole cell and for individual genes. The term ‘wild type’ may thus also include cells which have been genetically modified in other aspects (i.e. with regard to one or more genes) but not in relation to the genes of interest. The term “wild type” therefore does not include such cells where the gene sequences of the specific genes of interest have been altered at least partially by man using recombinant methods. A wild type cell according to any aspect of the present invention thus refers to a cell that has no genetic mutation with respect to the whole genome and/or a particular gene. Therefore, in one example, a wild type cell with respect to enzyme E1may refer to a cell that has the natural/non-altered expression of the enzyme E1in the cell. The wild type cell with respect to enzyme E2, E3, E4, E5, E6, E7, E8, E9, E10, E11, E12, E13, E14, etc. may be interpreted the same way and may refer to a cell that has the natural/non-altered expression of the enzyme E2, E3, E4, E5, E6, E7, E8, E9, E10, E11, E12, E13, E14, etc. respectively in the cell. A skilled person would be able to use any method known in the art to genetically modify a cell or microorganism. According to any aspect of the present invention, the genetically modified cell may be genetically modified so that in a defined time interval, within 2 hours, in particular within 8 hours or 24 hours, it forms at least twice, especially at least 10 times, at least 100 times, at least 1000 times or at least 10000 times more propanol and/or propionic acid than the wild-type cell. The increase in product formation can be determined for example by cultivating the cell according to any aspect of the present invention and the wild-type cell each separately under the same conditions (same cell density, same nutrient medium, same culture conditions) for a specified time interval in a suitable nutrient medium and then determining the amount of target product (alcohol with at least 3 carbons) in the nutrient medium. The term “second microorganism” or “third microorganism” or “fourth microorganism”, refers to a microorganism that is different from “the first microorganism” according to any aspect of the present invention. The numbers do not refer to the number of organisms that may be involved in the method according to any aspect of the present invention. For example, in a method of producing a higher alcohol according to any aspect of the present invention, a “first microorganism”, a “second microorganism” and a “fourth microorganism” are involved. There may not be a “third microorganism” involved in the method of producing higher alcohols according to any aspect of the present invention. The use of the numbers to refer to the microorganisms may thus be taken to differentiate between the different microorganisms involved according to any aspect of the present invention but not to the number of organisms involved. For example, the use of “fourth microorganism” in the method according to any aspect of the present invention does not mean there are four organisms used in the method but that the specific fourth microorganism may be present in the method. In one example, where propanol is the alcohol produced according to any aspect of the present invention, the first, second and third microorganism may be present in one fermenter in the presence of oxygen. The product, aqueous medium comprising the propanol may then be transferred to another vessel for carrying out (b) extraction using the extracting medium. In a further example, where propanol is the alcohol produced according to any aspect of the present invention, the first, second and third microorganism may be present in one fermenter in the presence of oxygen. The product, propanol in the aqueous medium may then be directly extracted from the medium containing the cells. The cells may then be recycled for production of the propanol. In one example, where propanol is the alcohol produced according to any aspect of the present invention, the first and second microorganism may be present in a first fermenter and the third microorganism in a second fermenter. In fermenter 1, the first and second microorganisms come in contact with the carbon source to produce acetate and/or ethanol. Ethanol and/or acetate may then be brought into contact with a third microorganism in fermenter 2 to produce propanol. A cycle may be created wherein the acetate and/or ethanol produced in fermenter 1 may be regularly fed into fermenter 2, the acetate and/or ethanol in fermenter 2 may be converted to propanol. Oxygen may be added into fermenter 2 to enable the third microorganism to convert acetate to propanol. Similarly, in fermenter 1 the first and second microorganism may come in contact with the carbon source comprising CO to produce acetate and/or ethanol. Ethanol and/or acetate may then be brought into contact with a third microorganism in fermenter 2 to produce propanol. A cycle may be created wherein the acetate and/or ethanol produced in fermenter 1 may be regularly fed into fermenter 2, the acetate and/or ethanol in fermenter 2 may be converted to propanol. CO fed into fermenter 1 may be transferred into fermenter 2 together with the acetate and/or ethanol. No special extraction method may be needed between the two fermenters. In one example, where a higher alcohol is produced according to any aspect of the present invention, the first, second and fourth microorganism may be present in a first fermenter. In the fermenter the first and second microorganisms come in contact with the carbon source first to produce acetate and/or ethanol. Ethanol and/or acetate then contacts the fourth microorganism in the same fermenter to produce at least one acid. The acid then contacts then produces at least one corresponding higher alcohol when it contacts the first and second microorganisms again. No special extraction method may be needed as the fourth microorganism has surprisingly been found to convert acetate and/or ethanol to at least one acid in the presence of CO. In a further example, where a higher alcohol is produced according to any aspect of the present invention, the first and second microorganism may be present in a first fermenter and the fourth microorganism in a second fermenter. In fermenter 1, the first and second microorganisms come in contact with the carbon source to produce acetate and/or ethanol. Ethanol and/or acetate may then be brought into contact with a fourth microorganism in fermenter 2 to produce at least one acid. The acid may then be fed back into fermenter 1 to produce at least one higher alcohol. A cycle may be created wherein the acetate and/or ethanol produced in fermenter 1 may be regularly fed into fermenter 2, the acetate and/or ethanol in fermenter 2 may be converted to at least one acid and the acid in fermenter 2 fed back into fermenter 1. Similarly, in fermenter 1 the first and second microorganism may come in contact with the carbon source comprising CO to produce acetate and/or ethanol. Ethanol and/or acetate may then be brought into contact with a fourth microorganism in fermenter 2 to produce at least one acid. The acid may then be optionally extracted and fed back into fermenter 1 to convert the acid to the desired higher alcohol. A cycle may be created wherein the acetate and/or ethanol produced in fermenter 1 may be regularly fed into fermenter 2, the acetate and/or ethanol in fermenter 2 may be converted to at least one acid and the acid in fermenter 2 fed back into fermenter 1. CO fed into fermenter 1 may be transferred into fermenter 2 together with the acetate and/or ethanol. No special extraction method may be needed as the fourth microorganism has surprisingly been found to convert acetate and/or ethanol to at least one acid in the presence of CO. In yet another example, where a higher alcohol is produced according to any aspect of the present invention, the media is being recycled between fermenters 1 and 2. Therefore, the ethanol and/or acetate produced in fermenter 1 may be fed into fermenter 2 and the acid produced in fermenter 2 may be fed back into fermenter 1. In the process of recycling the media, CO from fermenter 1 may be introduced into fermenter 2. Also, the acids produced in fermenter 2 may be consequently reintroduced into fermenter 1. The fourth microorganisms in fermenter 2 may be able to continue producing acids from acetate and ethanol in the presence of the CO recycled from fermenter 1 into fermenter 2. The accumulated higher alcohols in fermenters 1 and 2 may then be extracted by means known in the art. In a further example, there may be three containers present to carry out the method according to any aspect of the present invention. The first and second microorganism may be present in a first fermenter, the fourth microorganism in a second fermenter and a third fermenter with the first and second microorganisms. In fermenter 1, the first and second microorganisms come in contact with the carbon source to produce acetate and/or ethanol. Ethanol and/or acetate may then be brought into contact with a fourth microorganism in fermenter 2 to produce at least one acid. The acid may then be fed into fermenter 3 to produce at least one higher alcohol. In the production of the acid and/or higher alcohol from the carbon source a combination of bacteria may be used. There may be more than one acetogenic bacteria present in combination with one or more fourth microorganisms. In another example, there may be more than one type of acetogenic bacteria present and only one type of fourth microorganism. In yet another example, there may be more than one fourth microorganism present in combination with only one acetogenic bacteria. The phrase ‘the genetically modified cell has an increased activity, in comparison with its wild type, in enzymes’ as used herein refers to the activity of the respective enzyme that is increased by a factor of at least 2, in particular of at least 10, more in particular of at least 100, yet more in particular of at least 1000 and even more in particular of at least 10000. The phrase “increased activity of an enzyme”, as used herein is to be understood as increased intracellular activity. Basically, an increase in enzymatic activity can be achieved by increasing the copy number of the gene sequence or gene sequences that code for the enzyme, using a strong promoter or employing a gene or allele that codes for a corresponding enzyme with increased activity and optionally by combining these measures. Genetically modified cells used in the method according to the invention are for example produced by transformation, transduction, conjugation or a combination of these methods with a vector that contains the desired gene, an allele of this gene or parts thereof and a vector that makes expression of the gene possible. Heterologous expression is in particular achieved by integration of the gene or of the alleles in the chromosome of the cell or an extrachromosomally replicating vector. Similarly, a decreased activity of an enzyme refers to decreased intracellular activity. In one example, the increased expression of an enzyme according to any aspect of the present invention may be 5, 10, 15, 20, 25, 30, 25, 40, 45, 50, 55, 60, 65, 70, 75, 80, 85, 90, 95 or 100% more relative to the expression of the enzyme in the wild type cell. Similarly, the decreased expression of an enzyme according to any aspect of the present invention may be 5, 10, 15, 20, 25, 30, 25, 40, 45, 50, 55, 60, 65, 70, 75, 80, 85, 90, 95 or 100% less relative to the expression of the enzyme in the wild type cell. The culture medium to be used must be suitable for the requirements of the particular strains. Descriptions of culture media for various microorganisms are given in “Manual of Methods for General Bacteriology”. All percentages (%) are, unless otherwise specified, mass percent. With respect to the source of substrates comprising carbon dioxide and/or carbon monoxide, a skilled person would understand that many possible sources for the provision of CO and/or CO2as a carbon source exist. It can be seen that in practice, as the carbon source of the present invention any gas or any gas mixture can be used which is able to supply the microorganisms with sufficient amounts of carbon, so that acetate and/or ethanol, may be formed from the source of CO and/or CO2. Generally for the cell of the present invention the carbon source comprises at least 50% by weight, at least 70% by weight, particularly at least 90% by weight of CO2and/or CO, wherein the percentages by weight—% relate to all carbon sources that are available to the cell according to any aspect of the present invention. The carbon material source may be provided. Examples of carbon sources in gas forms include exhaust gases such as synthesis gas, flue gas and petroleum refinery gases produced by yeast fermentation or clostridial fermentation. These exhaust gases are formed from the gasification of cellulose-containing materials or coal gasification. In one example, these exhaust gases may not necessarily be produced as by-products of other processes but can specifically be produced for use with the mixed culture of the present invention. According to any aspect of the present invention, the carbon source may be synthesis gas. Synthesis gas can for example be produced as a by-product of coal gasification. Accordingly, the microorganism according to any aspect of the present invention may be capable of converting a substance which is a waste product into a valuable resource. In another example, synthesis gas may be a by-product of gasification of widely available, low-cost agricultural raw materials for use with the mixed culture of the present invention to produce substituted and unsubstituted organic compounds. There are numerous examples of raw materials that can be converted into synthesis gas, as almost all forms of vegetation can be used for this purpose. In particular, raw materials are selected from the group consisting of perennial grasses such as miscanthus, corn residues, processing waste such as sawdust and the like. In general, synthesis gas may be obtained in a gasification apparatus of dried biomass, mainly through pyrolysis, partial oxidation and steam reforming, wherein the primary products of the synthesis gas are CO, H2and CO2. Syngas may also be a product of electrolysis of CO2. A skilled person would understand the suitable conditions to carry out electrolysis of CO2to produce syngas comprising CO in a desired amount. Usually, a portion of the synthesis gas obtained from the gasification process is first processed in order to optimize product yields, and to avoid formation of tar. Cracking of the undesired tar and CO in the synthesis gas may be carried out using lime and/or dolomite. These processes are described in detail in for example, Reed, 1981. Mixtures of sources can be used as a carbon source. According to any aspect of the present invention, a reducing agent, for example hydrogen may be supplied together with the carbon source. In particular, this hydrogen may be supplied when the C and/or CO2is supplied and/or used. In one example, the hydrogen gas is part of the synthesis gas present according to any aspect of the present invention. In another example, where the hydrogen gas in the synthesis gas is insufficient for the method of the present invention, additional hydrogen gas may be supplied. A skilled person would understand the other conditions necessary to carry out the method according to any aspect of the present invention. In particular, the conditions in the container (e.g. fermenter) may be varied depending on the first, second and third microorganisms used. The varying of the conditions to be suitable for the optimal functioning of the microorganisms is within the knowledge of a skilled person. In one example, the method according to any aspect of the present invention may be carried out in an aqueous medium with a pH between 5 and 8, 5.5 and 7. The pressure may be between 1 and 10 bar. The term “contacting”, as used herein, means bringing about direct contact between the cell according to any aspect of the present invention and the medium comprising the carbon source in step (a) and/or the direct contact between the third microorganism and the acetate and/or ethanol from step (a) in step (b). For example, the cell, and the medium comprising the carbon source may be in different compartments in step (a). In particular, the carbon source may be in a gaseous state and added to the medium comprising the cells according to any aspect of the present invention. In particular, the aqueous medium may comprise the cells and a carbon source comprising CO and/or CO2for step (a) to be carried out. More in particular, the carbon source comprising CO and/or CO2is provided to the aqueous medium comprising the cells in a continuous gas flow. Even more in particular, the continuous gas flow comprises synthesis gas. These gases may be supplied for example using nozzles that open up into the aqueous medium, frits, membranes within the pipe supplying the gas into the aqueous medium and the like. The overall efficiency, alcohol productivity and/or overall carbon capture of the method of the present invention may be dependent on the stoichiometry of the CO2, CO, and H2in the continuous gas flow. The continuous gas flows applied may be of composition CO2and H2. In particular, in the continuous gas flow, concentration range of CO2may be about 10-50%, in particular 3% by weight and H2would be within 44% to 84%, in particular, 64 to 66.04% by weight. In another example, the continuous gas flow can also comprise inert gases like N2, up to a N2concentration of 50% by weight. The term ‘about’ as used herein refers to a variation within 20 percent. In particular, the term “about” as used herein refers to +/−20%, more in particular, +/−10%, even more in particular, +/−5% of a given measurement or value. A skilled person would understand that it may be necessary to monitor the composition and flow rates of the streams at relevant intervals. Control of the composition of the stream can be achieved by varying the proportions of the constituent streams to achieve a target or desirable composition. The composition and flow rate of the blended stream can be monitored by any means known in the art. In one example, the system is adapted to continuously monitor the flow rates and compositions of at least two streams and combine them to produce a single blended substrate stream in a continuous gas flow of optimal composition, and means for passing the optimised substrate stream to the fermenter. Microorganisms which convert CO2and/or CO to acetate and/or ethanol, in particular acetate, as well as appropriate procedures and process conditions for carrying out this metabolic reaction is well known in the art. Such processes are, for example described in WO9800558, WO2000014052 and WO2010115054. The term “an aqueous solution” or “medium” comprises any solution comprising water, mainly water as solvent that may be used to keep the cell according to any aspect of the present invention, at least temporarily, in a metabolically active and/or viable state and comprises, if such is necessary, any additional substrates. The person skilled in the art is familiar with the preparation of numerous aqueous solutions, usually referred to as media that may be used to keep inventive cells, for example LB medium in the case of In particular, the reaction mixture according to any aspect of the present invention (i.e. mixture of the first microorganism—the acetogenic organism in log phase, the second microorganism—the acetogenic organism in stationary phase, the carbon source in the presence of oxygen can be employed in any known bioreactor or fermenter to carry out any aspect of the present invention. The reaction mixture may further comprise a fourth microorganism to result in higher alcohols being produced in the fermenter. In another example, the reaction mixture may further comprise a third microorganism to result in propanol being produced in the fermenter ‘Higher alcohols’ as used herein refers to alcohols that contain 4 to 10 carbon atoms and may be somewhat viscous, or oily, and have heavier fruity odours. Higher alcohols may include but are not limited to butanol, pentanol, hexanol, heptanol, octanol, nonanol, decanol and the like. More in particular, the higher alcohol may be selected from the group consisting of 1-butanol, 2-methyl-1-butanol, isobutanol, 3-methyl-1-butanol, 1-hexanol, 1-octanol, 1-pentanol, 1-heptanol, 3-methyl-1-pentanol, 4-methyl-1-hexanol, 5-methyl-1-heptanol, 4-methyl-1-pentanol, 5-methyl-1-hexanol, 6-methyl-1-heptanol and combinations thereof. According to any aspect of the present invention, the ‘corresponding higher alcohol’ refers to an alcohol with the same number of carbon atoms as that of the acid from which the corresponding higher alcohol is formed. For example, butanoic acid may be converted to the corresponding alcohol-butanol; hexanoic acid may be converted to the corresponding alcohol-hexanol; heptanoic acid may be converted to the corresponding alcohol-heptanol; octanoic acid may be converted to the corresponding alcohol-octanol; nonanoic acid may be converted to the corresponding alcohol-nonanol; decanoic acid may be converted to the corresponding alcohol-decanol and the like. The foregoing describes preferred embodiments, which, as will be understood by those skilled in the art, may be subject to variations or modifications in design, construction or operation without departing from the scope of the claims. These variations, for instance, are intended to be covered by the scope of the claims. Co-cultivation of A defined medium was used for the co-cultivation of both microorganisms consisting of 2 g/L (NH4)2HPO4, 0.2 g/L NaCl, 0.15 g/l KCl, 1 g/l KOH, 0.5 g/L MgCl2×6H2O, 0.2 g/L CaCl2)×2H2O, 15 mg/L FeCl2×4H2O, 0.4 g/L L-cysteine-HCl, 0.4 g/L Na2S×9H2O, 3 mg/L boric acid, 2 mg/L CoCl2×6H2O, 1 mg/L ZnSO4×7 H2O, 0.3 mg/L Na2MoO4×2 H2O, 0.3 mg/L MnSO4×H2O, 0.2 mg/L NiCl2×6 H2O, 0.1 mg/L CuCl2×2 H2O, 0.1 mg/L Na2SeO3, 106 μg/L biotin, 5 μg/L folic acid, 2.5 μg/L pyridoxine-HCl, 266 μg/L thiamine-HCl×H2O, 12.5 μg/L riboflavin, 12.5 μg/L nicotinic acid, 413 μg/L Ca-pantothenoic acid, 12.5 μg/L vitamine B12, 12.5 μg/L p-aminobenzoic acid, 15 μg/L lipioic acid. The autotrophic cultivation was performed in 250 mL defined medium in a 500 mL serum bottle that was continuously gassed with synthesis gas consisting of 67% H2and 33% CO2at a rate of 1 L/h. The gas was introduced into the liquid phase by a microbubble disperser with a pore diameter of 10 μm. The serum bottle was continuously shaken in an open water bath Innova 3100 from New Brunswick Scientific at 37° C. and a shaking rate of 150 min−1. The pH was held in a range of pH 5.0-6.5 by continuous addition of an anaerobic stock solution of KOH (40 g/L). At the beginning of the experiment, Parallel to that, During the experiment samples of 5 mL were taken for the determination of OD600, pH and product concentrations. The latter were determined by quantitative1H-NMR-spectroscopy. After inoculation of Co-Cultivation of A complex medium was used for the co-cultivation of both microorganisms consisting of 1 g/L NH4Cl, 0.1 g/L KCl, 0.2 g/L MgSO4×7 H2O, 0.8 g/L NaCl, 0.1 g/L KH2PO4, 20 mg/L CaCl2×2 H2O, 20 g/L MES, 1 g/L yeast extract, 0.4 g/L L-cysteine-HCl, 0.4 g/L Na2S×9H2O, 20 mg/L nitrilotriacetic acid, 10 mg/L MnSO4×H2O, 8 mg/L (NH4)2Fe(SO4)2×6 H2O, 2 mg/L CoCl2×6 H2O, 2 mg/L ZnSO4×7 H2O, 0.2 mg/L CuCl2×2 H2O, 0.2 mg/L Na2MoO4×2 H2O, 0.2 mg/L NiCl2×6 H2O, 0.2 mg/L Na2SeO4, 0.2 mg/L Na2WO4×2 H2O, 20 μg/L biotin, 20 μg/L folic acid, 100 μg/L pyridoxine-HCl, 50 μg/L thiamine-HCl×H2O, 50 μg/L riboflavin, 50 μg/L nicotinic acid, 50 μg/L Ca-pantothenoic acid, 1 μg/L vitamine B12, 50 μg/L p-aminobenzoic acid, 50 μg/L lipoic acid. The autotrophic cultivation was performed in 500 mL complex medium in a 1 L serum bottle that was continuously gassed with synthesis gas consisting of 5% H2, 25% CO2, 25% CO and 45% N2at a rate of ˜12 L/h (≥0.5 ppm). The gas was introduced into the liquid phase by a microbubble disperser with a pore diameter of 10 μm. The serum bottle was continuously shaken in an open water bath Innova 3100 from New Brunswick Scientific at 37° C. and a shaking rate of 120 min−1. The pH was not controlled during this experiment. At the beginning of the experiment, Parallel to that, During the experiment samples of 5 mL were taken for the determination of OD600, pH and product concentrations. The latter were determined by quantitative1H-NMR-spectroscopy. After inoculation of Growth and Production of Acetate and Ethanol by For the biotransformation of hydrogen and carbon dioxide to acetic acid and ethanol the homoacetogenic bacterium For the preculture 500 ml medium (ATCC1754-medium: pH=6.0; 20 g/L MES; 1 g/L yeast extract, 0.8 g/L NaCl; 1 g/L NH4Cl; 0.1 g/L KCl; 0.1 g/L KH2PO4; 0.2 g/L MgSO4×7 H2O; 0.02 g/L CaCl2×2 H2O; 20 mg/L nitrilotriacetic acid; 10 mg/L MnSO4×H2O; 8 mg/L (NH4)2Fe(SO4)2×6 H2O; 2 mg/L CoCl2×6 H2O; 2 mg/L ZnSO4×7 H2O; 0.2 mg/L CuCl2×2 H2O; 0.2 mg/L Na2MoO4×2 H2O; 0.2 mg/L NiCl2×6 H2O; 0.2 mg/L Na2SeO4; 0.2 mg/L Na2WO4×2 H2O; 20 μg/L d-biotin; 20 μg/L folic acid; 100 μg/L pyridoxine-HCl; 50 μg/L thiamine-HCl×H2O; 50 μg/L riboflavin; 50 μg/L nicotinic acid; 50 μg/L Ca-pantothenate; 1 μg/L vitamin B12; 50 μg/L p-aminobenzoate; 50 μg/L lipoic acid; approx. 67.5 mg/L NaOH) with additional 400 mg/L L-cysteine-hydrochlorid and 400 mg/L Na2S×9H2O were inoculated with 5 mL of a frozen cryo stock of After the precultivation, the cell suspension was centrifuged (10 min, 4200 rpm) and the pellet was resuspended in fresh medium. For the main culture, as many cells from the preculture as necessary for an OD600nmof 0.1 were transferred in 500 mL medium with additional 400 mg/L L-cysteine-hydrochlorid. The chemolithoautotrophic cultivation was carried out in a 1 L pressure-resistant glass bottle at 37° C., 150 rpm and a ventilation rate of 1 L/h with a premixed gas with 66.95% H2, 33% CO2, 0.05% O2in an open water bath shaker for 41 h. The gas was discharged into the medium through a sparger with a pore size of 10 μm, which was mounted in the center of the reactors. Culturing was carried out with no pH control. During cultivation several 5 mL samples were taken to determinate OD600nm, pH and product formation. The determination of the product concentrations was performed by semiquantitative 1H-NMR spectroscopy. As an internal quantification standard sodium trimethylsilylpropionate (T(M)SP) was used. Also the dissolved oxygen in the cultivation medium was measured online by oxygen dipping probes (PSt6 with Oxy4Trace, Presens, Germany). During the cultivation period cell growth was observed by an increase of the OD600nmfrom 0.08 to 0.76 in 41 h, which correlates with a growth rate of p=0.054 h−1. The concentration of acetate increased from 37 mg/L to 6600 mg/L and the concentration of ethanol increased from 4 mg/L to 120 mg/L. Over the whole cultivation period the dissolved oxygen concentration was 0.00 mg/L. In a similar technical setting with the same parameters (medium composition, volume, bottle, gas, ventilation rate, temperature, shaking frequency), but without cells in the medium, a dissolved oxygen concentration of 0.01 mg/L was measured. Growth and Acetate Production by For the biotransformation of hydrogen and carbon dioxide to acetic acid the homoacetogenic bacterium For the preculture 500 ml medium (ATCC1754-medium: pH=6.0; 20 g/L MES; 1 g/L yeast extract, 0.8 g/L NaCl; 1 g/L NH4Cl; 0.1 g/L KCl; 0.1 g/L KH2PO4; 0.2 g/L MgSO4×7 H2O; 0.02 g/L CaCl2×2 H2O; 20 mg/L nitrilotriacetic acid; 10 mg/L MnSO4×H2O; 8 mg/L (NH4)2Fe(SO4)2×6 H2O; 2 mg/L CoCl2×6 H2O; 2 mg/L ZnSO4×7 H2O; 0.2 mg/L CuCl2×2 H2O; 0.2 mg/L Na2MoO4×2 H2O; 0.2 mg/L NiCl2×6 H2O; 0.2 mg/L Na2SeO4; 0.2 mg/L Na2WO4×2 H2O; 20 μg/L d-biotin; 20 μg/L folic acid; 100 μg/L pyridoxine-HCl; 50 μg/L thiamine-HCl×H2O; 50 μg/L riboflavin; 50 μg/L nicotinic acid; 50 μg/L Ca-pantothenate; 1 μg/L vitamin B12; 50 μg/L p-aminobenzoate; 50 μg/L lipoic acid; approx. 67.5 mg/L NaOH) with additional 400 mg/L L-cysteine-hydrochlorid and 400 mg/L Na2S×9H2O were inoculated with 5 mL of a frozen cryo stock of After the precultivation, the cell suspension was centrifuged (10 min, 4200 rpm) and the pellet was washed with 10 ml medium and centrifuged again. For the main culture, as many washed cells from the preculture as necessary for an OD600nmof 0.1 were transferred in 200 mL medium with additional 400 mg/L L-cysteine-hydrochlorid. The chemolithoautotrophic cultivation was carried out in a 250 mL pressure-resistant glass bottles at 37° C., 150 rpm and a ventilation rate of 1 L/h with a premixed gas with 66.85% H2, 33% CO2, 0.15% O2in an open water bath shaker for 47 h. The gas was discharged into the medium through a sparger with a pore size of 10 μm, which was mounted in the center of the reactors. Culturing was carried out with no pH control. During cultivation several 5 mL samples were taken to determinate OD600nm, pH and product formation. The determination of the product concentrations was performed by semiquantitative 1H-NMR spectroscopy. As an internal quantification standard sodium trimethylsilylpropionate (T(M)SP) was used. Also the dissolved oxygen in the cultivation medium was measured online by oxygen dipping probes (PSt6 with Oxy4Trace, Presens, Germany). During the cultivation period cell growth was observed by an increase of the OD600nmfrom 0.10 to 0.45, which correlates with a growth rate of p=0.032 h−1. The concentration of acetate increased from 7 mg/L to 2347 mg/L and the concentration of ethanol increased from 2 mg/L to 319 mg/L. Over the whole cultivation period the dissolved oxygen concentration was 0.00 mg/L. In a similar technical setting with the same parameters (medium composition, volume, bottle, gas, ventilation rate, temperature, shaking frequency), but without cells in the medium, a dissolved oxygen concentration of 0.03 mg/L was measured. Production of propionic acid and propanol on synthesis gas with oxygen with For the biotransformation of hydrogen and carbon dioxide to propionic acid and propanol the homoacetogenic bacterium For the cultivation of For the main culture of For the first preculture of For a second preculture of For the co-cultivation culture, as many washed cells from the second preculture of During cultivation several 5 mL samples were taken to determinate OD600nm, pH and product formation. The determination of the product concentrations is performed by LCMS and semiquantitative1H-NMR spectroscopy. As an internal quantification standard sodium trimethylsilylpropionate (T(M)SP) was used. During the co-cultivation the concentration of acetate increased from 0.79 g/L to 1.83 g/L, for propionate from 0.00 g/L to 0.23 g/L, for propanol from 0 to 19 mg/L, for butyrate from 0 to 14 mg/L, and for formate from 32 mg/L to 335 mg/L. The concentration of ethanol decreased from 47 mg/L to 25 mg/L during this time. Production and Processing of Propanol from Synthesis Gas with For the biotransformation of hydrogen and carbon dioxide to propanol the homoacetogenic bacterium For the cultivation of For the main culture of During 187 h 4.6 g/L ethanol and 14.6 g/L acetate were produced. For the first preculture of For a second preculture of For the main culture 150 mL of the sterile-filtered medium from the main culture of During the cultivation time 2 g/L ethanol were consumed and 2 g/L propionate and 49 mg/L propanol were produced. During both cultivations several 5 mL samples were taken to determinate OD600nm, pH and product formation. The determination of the product concentrations was performed by HPLC and NMR spectroscopy. For extraction of propanol two 50 mL samples of the main culture with In both cases propanol was extracted completely into the organic phase. For the extraction of butanol, two separate extracting mediums were used (1) oleyl alcohol and (2) a mixture of polypropylene glycol and hexadecane (70/30% w/w) was used. For the extraction with 70% w/w polypropylene and 30% w/w hexadecane, 50 g of a butanol solution with 0.522 g/kg were shaken at ambient temperature (20-25° C.) in a separation funnel with 46 g of the extracting medium for two minutes. The two phases were separated and butanol concentration in the aqueous phase was determined. After extraction the butanol concentration in the aqueous phase had decreased to 0.251 g/kg. The distribution coefficient calculated by mass balance was 1.17. For the extraction with oleyl alcohol 4.81 g of a butanol solution with 5.97 g/kg were shaken at ambient temperature (20-25° C.) in a centrifuge tube with 2.02 g extractant for 30 minutes. The two phases were separated and the concentration in the aqueous phase was determined. After extraction butanol concentration in the aqueous phase had decreased to 2.40 g/kg. The distribution coefficient calculated by mass balance was 2.97. The present invention relates to a method of producing at least one alcohol from a carbon source, the method comprising: (a) producing the alcohol in an aqueous medium under aerobic conditions; and (b) extracting the alcohol from step (a) from the aqueous medium by: (bi) contacting the alcohol in the aqueous medium with at least one extracting medium for a time sufficient to extract the alcohol from the aqueous medium into the extracting medium, (bii) separating the extracting medium with the extracted alcohol from the aqueous medium wherein the extracting medium comprises:—oleyl alcohol; or—polypropylene glycol and an alkane wherein the alcohol comprises at least 3 carbon atoms. 1. A method of producing at least one alcohol from a carbon source, the method comprising:
(a) producing the alcohol in an aqueous medium under aerobic conditions; and (b) extracting the alcohol from (a) from the aqueous medium by:
(bi) contacting the alcohol in the aqueous medium with at least one extracting medium for a time sufficient to extract the alcohol from the aqueous medium into the extracting medium, and (bii) separating the extracting medium with the extracted alcohol from the aqueous medium, wherein the extracting medium comprises oleyl alcohol, the alcohol comprises at least 3 carbon atoms, and the carbon source comprises CO and/or CO2. 2. The method according to (ai) contacting the carbon source with a reaction mixture comprising
a first acetogenic microorganism in an exponential growth phase; free oxygen; and a second acetogenic microorganism in a stationary phase, wherein the first and second acetogenic microorganisms are capable of converting the carbon source to the acetate and/or ethanol; and (aii) contacting the acetate and/or ethanol from (ai) with a third microorganism capable of converting the acetate and/or ethanol to the propanol in the aqueous medium. 3. The method according to (a1) contacting the carbon source with a reaction mixture comprising
a first acetogenic microorganism in an exponential growth phase; free oxygen; and a second acetogenic microorganism in a stationary phase wherein the first and second acetogenic microorganisms are capable of converting the carbon source to the acetate and/or ethanol; (a2) contacting the acetate and/or ethanol from (a1) with a fourth microorganism capable of carrying out the ethanol carboxylate fermentation pathway and converting the acetate and/or ethanol from (a1) to form an acid; and (a3) contacting the acid from (a2) with the reaction mixture of (a1), wherein the first and/or second acetogenic microorganisms are capable of converting the acid to the corresponding higher alcohol in the aqueous medium, and wherein the higher alcohol comprises 4 to 10 carbon atoms. 4. The method according to 5. The method according to 6. The method according to 7. The method according to 8. The method according to 9. The method according to 10. The method according to 11. The method according to 12. The method according to 13. The method according to FIELD OF THE INVENTION
BACKGROUND OF THE INVENTION
DESCRIPTION OF THE INVENTION
EXAMPLES
Example 1
Results of Example 1 NMR-analytics Process Acetate, Ethanol, Butyrate, n-Butanol, Hexanoate, Hexanol, time, h pH OD600 mg/L mg/L mg/L mg/L mg/L mg/L 0.0 6.37 0.11 4 2 n.d. n.d. n.d. n.d. 19.5 5.49 0.12 818 10 n.d. n.d. n.d. n.d. 40.3 5.49 0.21 1930 51 n.d. n.d. n.d. n.d. 63.8 5.10 0.43 5005 160 n.d. n.d. n.d. n.d. 79.5 5.85 0.49 8444 260 n.d. n.d. n.d. n.d. 95.0 5.95 0.58 8984 291 n.d. n.d. n.d. n.d. 96.0 5.90 0.78 9299 316 16 n.d. 23 n.d. 121.5 6.28 0.71 11030 9 801 10 81 n.d. 142.3 6.29 0.71 12238 8 934 14 87 n.d. 160.3 6.30 0.67 13096 9 1006 16 103 n.d. 185.5 6.28 0.57 16860 13 1143 17 134 n.d. 209.0 6.23 0.52 19275 17 1163 16 136 n.d. 234.0 5.82 0.42 18368 19 991 15 108 n.d. 259.3 5.72 0.34 18744 20 1005 15 116 n.d. (n.d. = not detected) Example 2
Results of Example 2 NMR-analytics Process Acetate, Ethanol, Butyrate, n-Butanol, Hexanoate, Hexanol, time, h pH OD600 mg/L mg/L mg/L mg/L mg/L mg/L 0.0 6.13 0.11 26 3 n.d. n.d. n.d. n.d. 18.0 5.89 0.55 1063 18 n.d. n.d. n.d. n.d. 42.0 5.58 1.02 2353 79 n.d. n.d. n.d. n.d. 71.3 5.31 1.34 3081 534 n.d. n.d. n.d. n.d. 117.5 5.39 1.78 2612 1946 n.d. n.d. n.d. n.d. 162.0 5.87 1.88 665 4153 n.d. n.d. n.d. n.d. 192.0 6.02 1.85 43 4747 n.d. n.d. n.d. n.d. 240.0 6.03 1.19 28 4805 n.d. n.d. n.d. n.d. 240.0 6.03 1.17 3209 4775 134 n.d. 46 n.d. 258.0 6.24 1.22 1078 1727 46 522 1380 457 283.5 6.49 1.24 331 112 1380 690 1590 1478 330.0 6.50 0.80 343 110 1590 603 1344 1165 (n.d. = not detected) Example 3
Example 4
Example 5
Example 6
Example 7
Extraction of Butanol