23-03-2017 дата публикации
Номер: US20170081651A1
The present invention relates to a polypeptide having proline-specific endoprotease activity, selected from the group consisting of 1. A polypeptide having proline-specific endoprotease activity , selected from the group consisting ofi. a polypeptide comprising a mature polypeptide sequence of SEQ ID NO: 2;ii. a polypeptide that has least 70%, 75%, 80%, 85%, 86%, 87%, 88%, 89%, 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98% or 99% identity to the mature polypeptide sequence of SEQ ID NO: 2;iii. a polypeptide encoded by a nucleic acid that hybridizes under medium stringency, optionally under high stringency conditions to the complementary strand of the mature polypeptide coding sequence of SEQ ID NO:1;iv. a polypeptide encoded by a nucleic acid that has at least 80%, 85%, 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99% or 100% identity to the mature polypeptide coding sequence of SEQ ID NO: 1.2. A polypeptide that is an isolated claim 1 , substantially pure claim 1 , pure claim 1 , recombinant claim 1 , synthetic or variant polypeptide of the polypeptide of .3. A polypeptide according to claim 1 , wherein the mature polypeptide sequence of SEQ ID NO: 2 comprises amino acids 36 to 526 of SEQ ID NO: 2 claim 1 , wherein methionine at position 1 in SEQ ID NO: 2 is counted as 1.4. A composition comprising a polypeptide according to .5. A composition according to claim 4 , comprising a carrier claim 4 , an excipient claim 4 , or an auxiliary enzyme.6. A nucleic acid encoding a proline-specific endoprotease claim 4 , which has at least 70% claim 4 , 75% claim 4 , 80% claim 4 , 85% claim 4 , 90% claim 4 , 95% claim 4 , 96% claim 4 , 97% claim 4 , 98% claim 4 , or 99% sequence identity to the mature polypeptide encoding sequence of SEQ ID NO: 1.7. A nucleic acid that is an isolated claim 6 , substantially pure claim 6 , pure claim 6 , recombinant claim 6 , synthetic or variant nucleic acid of the nucleic acid of .8. An expression vector comprising a nucleic acid according to ...
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