Apparatus for liquid sample handling

Method and device to remove fluid and vapor

The present invention relates to a method and device for the removal of fluids and vapors in containment, from adjacent surfaces including supporting flat surfaces and substrates, such as protein spots printed in arrays and micro-array formats. This method and device removes fluids and vapors under controlled and repeatable conditions, enabling uniform phases for degree of drying incurred by objects, specimen and structures, including tissues, adsorbed particles and biological substrates, while reducing and preventing impact of meniscus phase surface tension forces.

A DEVICE FOR THE ADDITION OF A COMPOUND OR COMPOUND MIXTURE TO ANOTHER UNDER VARIOUS REACTION CONDITIONS, PARALLEL OR OTHERWISE

A device for the addition of a compound or compound mixture to another under various reaction conditions, parallel or otherwise is disclosed. The method and apparatus offer significant advantages over conventional experimental reactors, and is especially useful for synthesizing and screening large numbers of compounds. The device generally consists of a body (10) containing one or more recessed areas (14) surrounding one or more hollow pistons (18) mounted in barrels (30) covered by a top-plate (24). The recessed areas are filled with compounds. The hollow pistons are filled with other compounds. Using gas pressure the pistons move. This movement results in the addition of the compounds contained in the recessed areas to the compounds contained in the pistons. Modifications as found for conventional experimental reactors can also be incorporated in the apparatus.

APPARATUS FOR PIPETTING POWDER

The present invention provides apparatus and methods for collecting a plurality of measured quantities of a powdered material and dispensing each of the measured quantities to, for example, a multi-well vessel. Vacuum is used to collect the powdered material and at least one of gravity, a gas push, or a physical push is used to dispense the powdered material. Apparatus and methods of the invention are particularly useful for collection and delivery of powdered materials in high throughput chemical synthesis and biological assay environments where accurate measurement and dispensing of powders is critical.

A SYSTEM AND METHOD FOR HIGH THROUGHPUT SAMPLE PREPARATION AND ANALYSIS USING COLUMN CHROMATOGRAPHY

A system and method high throughput sample preparation and analysis using column chromatography. Each port in a plurality of ports has a port input th at interfaces with a first fluid source and a port output. A fluidic circuit is coupled to each port output and to a second fluid source, the fluidic circui t for controlling fluid flow from the plurality of ports and the second fluid source. The fluidic circuit is also coupled to a plurality of chromatography columns. An interface to an analyzer receives output from at least one of th e plurality of chromatography columns. The plurality of chromatography columns is moved relative to the analyzer via a translation stage, such that sample output from one of the plurality of chromatography columns can be selectivel y presented to the analyzer.

A SYSTEM AND METHOD FOR HIGH THROUGHPUT PROCESSING OF DROPLETS

A method for high throughput processing of a plurality of droplets. The droplets are dispensed onto a moving surface (1) and delayed in a delay line (11) in which the droplets hang from the moving surface for at least a specified minimum period of time. A laminate (6) may be spooled onto the moving surface (1) and each droplet may be dispensed onto the laminate (6). At least one operation is performed on each droplet from the group of operations consisting of mixing, diluting, concentration, heating, cooling, humidifying, filtering, and analyzing. The laminate (6) may then, in certain embodiments, be spooled off the moving surface (1), processed, and reused.

Biological micro-reactor suitable for high throughput screening, forms alternating mobile liquid reactors and liquid separators in parallel capillaries

L'invention concerne un dispositif microfluidique d'injection de trains de chambres mobiles de réaction (102, 103) avec séparateurs (101) non miscibles dans des micro-canaux (21 à 26), comprenant : - des moyens d'injection (10) pour injecter en alternance et en parallèle dans les micro-canaux du liquide pour former les chambres mobiles de réaction et du liquide pour former les séparateurs, - des moyens de contrôle de la progression de l'un des deux liquides, disposés pour intervenir sur une zone (31) de chaque micro-canal délimitant un volume a injecter de ce liquide, les moyens de contrôle étant aptes à provoquer l'arrêt ou le ralentissement de la progression de ce liquide sur la zone de chaque micro-canal en exerçant une action basée sur une propriété physico-chimique de ce liquide, cette action n'ayant pas d'effet direct sur l'autre liquide.

HIGH THROUGHPUT MECHANICAL ALLOYING AND SCREENING

The invention provides an apparatus and methods for conducting ball milling operations in a high throughput manner. Also provided are devices and methods for high throughput screening of alloys and other materials to identify those that have desired gas storage properties.

METHOD AND APPARATUS FOR USE IN SOLID-PHASE PHYSICAL, CHEMICAL, BIOLOGICAL AND BIOCHEMICAL TECHNIQUES

The method and apparatus which is suitable for performing a wide variety of processes involving the passage of a liquid through a solid-phase micro-particulate material consists of the micro-particulate material (16) being provided within a plunger (10) that is capable of sliding movement within a well (1). Downward movement of the plunger (10) into the well (1) forces any liquid contained in the well to pass into the plunger and through the micro-particulate material (16). Reversing the movement of the plunger results in the liquid, now within the plunger, to be drawn back into the well. Thus, the liquid passes twice through the micro-particulate material and is caused to move without any external pumping or vacuum system. The method and apparatus is particularly suited to plural processing and to micro-chromatographic procedures.

Method for isolation of independent, parallel chemical micro-reactions using a porous filter

The present invention relates to methods and apparatuses for conducting densely packed, independent chemical reactions in parallel in fluid-permeable arrays. Accordingly, this invention also focuses on the use of such arrays for applications such as DNA sequencing, most preferably pyrophosphate sequencing, and DNA amplification.

Methods and systems for positioning microspheres for imaging

Various methods and systems for positioning microspheres for imaging are provided. One system includes a filter medium that includes openings. The openings are spaced in a substantially equidistant manner across the filter medium. The system also includes a flow subsystem coupled to the filter medium. The flow subsystem is configured to exert a force on the microspheres such that the microspheres are positioned above the openings. A method for positioning microspheres for imaging includes exerting a force on the microspheres through a filter medium such that the microspheres are positioned above openings in the filter medium. The openings are spaced as described above.

Microfabricated, flowthrough porous apparatus for discrete detection of binding reactions

An improved microfabricated apparatus for conducting a multiplicity of individual and simultaneous binding reactions is described. The apparatus comprises a substrate on which are located discrete and isolated sites for binding reactions. The apparatus is characterized by discrete and isolated regions that extend through said substrate and terminate on a second surface thereof such that when a test sample is applied to the substrate, it is capable of penetrating through each such region during the course of said binding reaction. The apparatus is especially useful for sequencing by hybridization of DNA molecules.

A microarray assembly comprising a microporous membrane and an incubation chamber arrangement

Production of a thin film or powder array by liquid source misted chemical deposition comprises producing two or more types of metal precursor liquids; producing droplets; depositing the droplets on a substrate; and thermo-processing

Producing a thin film or powder array (I) by a liquid source misted chemical deposition process comprising melting a metal precursor into a solvent and producing two or more types of metal precursor liquids; producing droplets by applying high frequency to one of the liquid; transferring the droplets into a vacuum chamber; depositing the droplets on a substrate by a shutter or a moving mask; and thermo-processing the droplets, is new. Producing a thin film or powder array (I) by a liquid source misted chemical deposition process comprising: (a) melting a metal precursor (consisting of a material or catalyst) into a solvent and producing two or more types of metal precursor liquid; (b) selecting one type of liquid; (c) putting it into a reactor and producing droplets by applying high frequency to the liquid; transferring the droplets into a vacuum chamber at a given pressure; (d) depositing the droplets on each area of a substrate to have a concentration gradient by means of a shutter or ...

Reaktionsblock für parallele Synthesechemie und Gefäss dafür

EQUIPMENT AND PROCEDURE FOR THE POLYMER SYNTHESIS WITH STANDS

APPARATUS FOR THE EXECUTION OF BIOCHEMICAL REACTIONS

DEVICE AND PROCEDURE FOR THE SYNTHESIS OF POLYMERS WITH USE OF ARRAYS

REACTION BLOCK FOR PARALLEL SYNTHESIS CHEMISTRY AND CONTAINER FOR IT

METHOD AND SYSTEM FOR THE DETECTION OF CARDIAC RISK FACTORS

NOVEL INTEGRATED MICROARRAY ANALYSIS

REACTION BLOCK FOR PARALLEL SYNTHETIC CHEMISTRY AND VESSEL THEREFOR

A reaction vessel, particularly for use in parallel synthetic chemistry, is characterized by an inner structure, namely a reaction chamber and a discharge channel which are interconnected. The interconnection preferably consist of a connection channel between the bottom of the reaction chamber and the discharge channel so that almost any liquid in the reaction chamber can be removed through the discharge channel. The connection channel may be molded by an extension hinged to the core molding the reaction chamber, so that the reaction vessel can be conveniently manufactured by injection molding. A reaction block particularly for use with these reaction vessels comprises a closure means covering the open ends of the discharge channel and the reaction chamber of at least two of the reaction vessels. By guiding means the closure means is directed to perform a generally linear movement in moving from the opening to the closing position, and during this movement, to move towards the open ends ...

PARALLEL LOADING OF ARRAYS

... ²²²The present inventions provides an apparatus (20) and method for the parallel ²loading of multiple samples in a microarray (10) containing a plurality of sub-²arrays (14). The method makes use of a microarray (10) containing multiple sub-²arrays (14), a loading channel array (20), and a fluid handling robot or an ²assembly robot or machine.² ...

PARALLEL LOADING OF ARRAYS

The present inventions provides an apparatus (20) and method for the parallel loading of multiple samples in a microarray (10) containing a plurality of sub- arrays (14). The method makes use of a microarray (10) containing multiple sub- arrays (14), a loading channel array (20), and a fluid handling robot or an assembly robot or machine.

METHODS AND SYSTEMS FOR POSITIONING MICROSPHERES FOR IMAGING

Various methods and systems for positioning microspheres for imaging are provided. One system includes a filter medium that includes openings. The openings are spaced in a substantially equidistant manner across the filter medium. The system also includes a flow subsystem coupled to the filter medium. The flow subsystem is configured to exert a force on the microspheres such that the microspheres are positioned above the openings. A method for positioning microspheres for imaging includes exerting a force on the microspheres through a filter medium such that the microspheres are positioned above openings in the filter medium. The openings are spaced as described above.

Methods and systems for positioning microspheres for imaging

Various methods and systems for positioning microspheres for imaging are provided. One system includes a filter medium that includes openings. The openings are spaced in a substantially equidistant manner across the filter medium. The system also includes a flow subsystem coupled to the filter medium. The flow subsystem is configured to exert a force on the microspheres such that the microspheres are positioned above the openings. A method for positioning microspheres for imaging includes exerting a force on the microspheres through a filter medium such that the microspheres are positioned above openings in the filter medium. The openings are spaced as described above.

ASPIRING DEVICE ALLOWING TO DISSOCIATE FROM the FINE SOLID PARTICLES Of WITH a LIQUID

A device for separating the liquid and solid constituents in a chemical reaction medium (1), comprising a metal filtering needle (12) with lateral holes (5) able to pass through polymer- or rubber-like membranes without losing their impermeability to gas (2), a source of suction (7) able to draw liquid out of an enclosed space (8), a channel for evacuating liquid (6) and an end piece (4).

REACTION BLOCK ASSEMBLY FOR CHEMICAL SYNTHESIS

The reaction block assembly of the invention comprises a reaction block in which are disposed a plurality of reaction wells. Influent and effluent ducts open at the upper face of the reaction block adjacent each of the reaction wells. These ducts provide communication between each reaction well and a source of reagents and gases and with waste removal means for removal of effluent from the reaction wells. A membrane sheet overlies the upper face of the reaction block to define a pneumatic valve to open and close the duct openings each of the reaction wells to control the input of reagents and gases and effluent flow from the reaction wells. The reaction block assembly preferably includes a temperature control block that underlies the reaction block for control of the temperature in the reaction wells. A waster block underlies the reaction block or, if used, the temperature control block. The effluent ducts from the reaction wells communicate with the waste block for removal of reaction ...

Method for polymer synthesis using arrays

A polymer synthesis apparatus (20) for building a polymer chain including a head assembly (21) having an array of nozzles (22) with each nozzle coupled to a reservoir (23) of liquid reagent (24), and a base assembly (25) having an array of reaction wells (26). A transport mechanism (27) aligns the reaction wells (26) and selected nozzles (22) for deposition of the liquid reagent (24) into selected reaction wells (26). A sliding seal (30) is positioned between the head assembly (21) and the base assembly (25) to form a common chamber (31) enclosing both the reaction well (26) and the nozzles (22) therein. A gas inlet (70) into the common chamber (31), upstream from the nozzles (22), and a gas outlet: (71) out of the common chamber (31), downstream from the nozzles (22), sweeps the common chamber (31) of toxic fumes emitted by the reagents. Each reaction well (26) includes an orifice (74) extending into the well (26) which is of a size and dimension to form a capillary liquid seal to retain ...

Bead trapping device

A bead trapping device for trapping beads. The device is formed from a substrate, posts extending from the substrate; the posts being patterned to form cavities between the posts, the cavities having a diameter between 0.5 mum and 200 mum; and means for loading beads onto the substrate. A method of trapping microscopic beads for analysis, the method comprising the steps of contacting a fluid containing beads with a substrate having posts extending from the substrate to form bead trapping cavities, the bead trapping cavities having a diameter between 0.5 mum and 200 mum; and loading the beads into the bead trapping cavities.

Reaction plenum

An apparatus for facilitating the running of multiple simultaneous solid phase reactions for mounting on a temperature controllable water bath and a method of using this apparatus in the presence or absence of paramagnetic beads to perform chemical syntheses resulting in separation and recovery of a final desired reaction product is provided. The apparatus comprises a reaction plate, a reaction vessel for mounting in the reaction plate, a reaction plate holder, a screw-like rod, a mounting block attached to the reaction plate holder for operably receiving the screw-like rod, a motor, a sonication region in the water bath and a magnetic separation region in the water bath. Paramagnetic beads having reaction sites are introduced into the reaction vessels along with the appropriate solvent and one or more reactants. The contents of the reaction vessel are sonicated, then moved to the magnetic separation region where the paramagnetic beads are tightly held against the reaction vessel while ...

Pipetting device with pipette tip for solid phase reactions

A pipetting device for use with a pipette tip containing a solid phase reaction support, comprising a cylinder, a plunger movable within the cylinder between a retracted and an extended position, and a seal disposed between the plunger and cylinder for holding the plunger as it moves. The plunger has a central passageway within an upper portion of the plunger communicating with a side egress port on the plunger, the plunger movable between a retracted position, for aspirating and dispensing liquid reagent wherein the port remains above the seal, and an inserted position wherein the port is positioned below the seal for allowing fluid (e.g., washing solution or inert gas) to be introduced into the cylinder and tip. In an apparatus and method, an array of such devices is used, each fitted with a pipette tip having a solid support retained therein, for performing high-throughput and parallel chemical synthesis of biopolymers (e.g., oligonucleotides or peptides). In one aspect, means are provided ...

Device for capturing beads and method and apparatus for arraying beads

Liquid sample handling or transferring device

Apparatus for liquid sample handling comprises: <SL> <LI>(a) a plurality of hollow capillaries, each being open at both ends and of defined internal volume, wherein on contact of an open end of a capillary with a sample, said defined volume of sample is drawn up into the capillary by capillary action; <LI>(b) a housing which retains the capillaries in their desired orientation; and optionally <LI>(c) means to effect sample removal from the capillaries. </SL> The capillaries may be in a row or array. Sample removal may be via pressure application. The capillaries may be surface treated.

Process

NOVEL INTEGRATED MICROARRAY ANALYSIS

The present invention relates to methods for identifying analyte nucleic acids present in a sample comprising nucleic acid molecules, said method comprising the steps of: (a) providing a porous substrate, said substrate comprising a plurality of micro-channels, wherein said micro-channels have immobilized thereon a target molecule capable of binding to an analyte present in said sample; wherein said channels are further provided with analyte amplification components, a reporter system; and said sample; (b) amplifying analyte nucleic acid molecules present within said sample; and (c) allowing binding to take place between an amplified analyte nucleic acid obtained in step (b), said target molecule immobilized onto the channels of said substrate and said reporter system, wherein said reporter system allows detecting whether binding has occurred between said target molecule and said analyte nucleic acid. The present invention further relates to the use of said methods as well as kits for carrying ...

METHOD AND DEVICE TO REMOVE FLUID AND VAPOR

The present inventioti relates to a method and device for the removal of tluids and vapors in containment, fronl aiijacent surtaces including supporting flat surfaces and substrates, such as protein spots printed in arravs atid micro-array fot-mats. This method and device removes fluids and vapors under controlled and repeatable cunditions, enabling uuifortn phases for degree of drying, incurred bv objects, specimetl and structures. including tissucs, adsorbed particles and biological sttbstrates, while reducing and preventing impact of ineniscus phase surface tension forces.

METHOD OF PRODUCING THIN FILM OR POWDER ARRAY USING LIQUID SOURCE MISTED CHEMICAL DEPOSITION PROCESS USING REACTION AND DIFFUSION BETWEEN LIQUID AND LIQUID

PURPOSE: A method of producing a thin film or a powder array using a liquid source misted chemical deposition process is provided to form the thin film or the power array having an uniform phase by using a liquid mixing method at the room temperature. CONSTITUTION: Two or more metal precursor solutions are fabricated by resolving a metal precursor into a solvent. A liquid source is generated by applying a high frequency to the solution selected from the metal precursor solutions within a reactor. The liquid source is transferred to a vacuum chamber under the constant pressure. The liquid source is deposited on each region of a substrate by using a folding screen or a shifting mask. A thin film or a powder array is fabricated by performing a thermal process. The fabrication processes are performed by using the remaining solution. © KIPO 2005 ...

Vorrichtung und Verfahren zur Synthese von Polymeren mit Anwendung von Arrays

TEST DOSING EQUIPMENT WITH MULTIPLE CHANNELS AS WELL AS DOSING PROCEDURES

MICRO FLUIDIC CHIP, WHICH IS QUALIFIED TO SYNTHESIS OF RADIOACTIVELY MARKED MOLECULES IN A FOR THE IMAGING AT HUMANS OF MEANS POSITRON EMISSION TOPOGRAPHY SUITABLE YARDSTICK

COMBINATORIAL CHEMICAL SYNTHESIS

Combinational synthesis methods obtain a plurality of compositions having materially different characteristics using an apparatus having a plurality of collectors. A first quantity of fluid reactants are reacted to form a first quantity of production composition. Following completion of the collection of the first quantity of product composition, a second quantity of fluid reactants are reacted to form a second quantity of product composition materially different from the first quantity of product composition. An apparatus includes a nozzle (130) connected to a reactant source (120) and a plurality of collectors (236). The nozzle and plurality of collectors move relative to each other such that a collector can be selectively placed to receive a fluid stream emanating from the nozzle. The plurality of product compositions can be evaluated to determine their suitability for various applications. © KIPO & WIPO 2007 ...

REACTION BLOCK ASSEMBLY FOR CHEMICAL SYNTHESIS

The reaction block assembly (20) comprises a reaction block (12) containing a plurality of reaction wells (14). Influent and effluent ducts (38, 38', 39, 39') open at the upper face of the reaction block adjacent each to each of the reaction wells. These ducts provide communication between each reaction well and a source of reagents and gases and with waste removal means for removal of effluent from the reaction wells. A membrane sheet (28) overlies the upper face of the reaction block to define a pneumatic valve to open and lose the duct openings. The reaction block assembly preferably includes a temperature control block (16) that underlies the reaction block for control of the temperature in the reaction wells. Overlying the reaction block is a top manifold plate (18) block for delivery of high pressure gas to actuate the pneumatic valves by applying pressure against the membrane to seal the duct openings.

SYSTEM AND METHOD FOR HIGH THROUGHPUT SCREENING OF DROPLETS

A system and method for high throughput screening of fluid samples. A reduced pressure is applied, via an injection valve, to a sample aspiration tube. A first fluid and a second fluid are alternatively aspired, via the sample aspiration tube, the first fluid for filling a sample loop with samples, the second fluid for flushing the sample aspiration tube. Excess fluid aspirated from the first fluid source and all fluid aspirated from the second fluid source is captured in an inline trap.

Method for isolation of independent, parallel chemical micro-reactions using a porous filter

The present invention relates to methods and apparati for conducting densely packed, independent chemical reactions in parallel in a substantially two-dimensional array. Accordingly, this invention also focuses on the use of this array for applications such as DNA sequencing, most preferably pyrosequencing, and DNA amplification.

Apparatus and method for combinatorial chemistry synthesis

In a first embodiment, this invention includes an integrated robot apparatus for performing combinatorial chemistry synthesis protocols and having interchangeable work-stations, robot arm tools, and reaction vessels and reaction vessel arrays. The work-stations and tools are specialized to perform tasks necessary for the synthesis in a plurality of the reaction vessels grouped in a plurality of the reaction vessel arrays. Preferably, these elements function interchangeably because they have standardized sizes and conformation. The work-stations and tools include those for fluid dispensing or aspirating from individual reaction vessels or from all the reaction vessels in an array simultaneously. The reaction vessels can include, alternatively, stackable, ball-sealed reaction vessels, microtitre-like reaction vessel arrays, arrays of independent reaction vessels, valve-sealed reaction vessels, septum-sealed reaction vessels, and syringe reaction vessels. In alternative embodiments, this invention ...

Microfluidic chip capable of synthesizing radioactively labeled molecules on a scale suitable for human imaging with positron emission tomography

Described herein are automated, integrated microfluidic device comprising a chemical reaction chip comprising for performing chemical reaction, a microscale column integrated with the chip and configured for liquid flow from the column to at least one flow channel, and wherein the fluid flow into the column is controlled by on-chip valves; and comprising at least two on-chip valves for controlling fluid flow in the microfluidic device.

Wicking-based reagent-solution draining in an automated system

Embodiments of the present invention include processing steps and subsystems, within automated-biopolymer-synthesis systems and within other automated systems for organic-chemistry-based processing, for removing reagent solutions and solvents from reaction chambers following various synthetic reaction steps and washing steps undertaken during biopolymer synthesis. Embodiments of the present invention employ any of various different types of liquid-absorbing materials to wick, or remove by capillary action, liquids from reaction chambers. Wicking-based methods and subcomponents of the present invention remove significantly greater fractions of solutions from reaction chambers than conventional methods and subsystems and, in addition, are mechanically simpler and produce fewer deleterious side effects than currently used methods and subsystems.

Device for powder metering for chemical production processes under clean-room conditions, use thereof and metered addition method

Device for metering powder, in particular in clean-rooms, which includes a vessel containing powder and a sealing head with a septum for the vessel, wherein the sealing head is connectable powder-tight with the vessel and the septum powder-tight with the sealing head and the device further includes a vessel holder, which serves to hold the sealing head of the vessel, and the vessel with its opening points downwards, so that the powder can flow out of the vessel, wherein a gap is provided between the sealing head and a holding bowl of the vessel holder, in which a gas flow between the holding bowl and the sealing head can be created. The invention also relates to a use of the device and a metered addition method.

MULTI-CHANNEL REAGENT DISPENSING APPARATUS AND METHOD

Embodiments of the present invention are directed to an improved chemical synthesis apparatus for performing chemical synthesis such as nuclei acid synthesis in a plurality of reaction wells or cells in an efficient manner. The apparatus employs dispenser heads (16) that each include a cluster of nozzles (14) which are fluidicly coupled to a plurality of reagent sources (12) for dispensing different reagents through the single dispenser head. Because each dispenser head (16) is capable of dispensing a plurality of different reagents, the apparatus can perform simultaneous synthesis in a plurality of cells at a high throughput without complex and elaborate control of movement of the dispenser heads (16) relative to the cells.

Contact unit comprising at least one reaction container integrated therein or attached thereto

APPARATUS FOR PIPETTING POWDER

REACTION BLOCK FOR PARALLEL SYNTHETIC CHEMISTRY AND VESSEL THEREFOR

A reaction vessel, particularly for use in parallel synthetic chemistry, is characterized by an inner structure, namely a reaction chamber and a discharge channel,which are interconnected. The interconnection preferably consist of a connection channel between the bottom of the reaction chamber and the discharge channel so that almost any liquid in the reaction chamber can be removed through the discharge channel. The connection channel may be molded by an extension hinged to the core molding the reaction chamber, so that the reaction vessel can be conveniently manufactured by injection molding. A reaction block particularly for use with these reaction vessels comprises a closure means covering the open ends of the discharge channel and the reaction chamber of at least two of the reaction vessels. By guiding means the closure means is directed to perform a generally linear movement in moving from the opening to the closing position, and during this movement, to move towards the open ends ...

MICROREACTOR

The invention is directed to a porous support for conducting one or more chemical reactions comprising a polymeric or silica porous monolith functionalised with an agent. In one embodiment, the agent is a catalytic or scavenging agent. The invention also relates to microreactors comprising such porous supports for use in conducting chemical reactions including organic chemical reactions, metal scavenging, diagnostic reactions or catalysis reactions. The novel porous supports described herein find particular application in small scale high throughput screening.

Microfluidic chip capable of synthesizing radioactively labeled molecules on a scale suitable for human imaging with positron emission tomography

Described herein are automated, integrated microfluidic device comprising a chemical reaction chip comprising for performing chemical reaction, a microscale column integrated with the chip and configured for liquid flow from the column to at least one flow channel, and wherein the fluid flow into the column is controlled by on-chip valves; and comprising at least two on-chip valves for controlling fluid flow in the microfluidic device.

Solid phase synthesis reaction vessel and method of using the same

A solid phase synthesis reaction vessel includes a vessel with a first inlet, a second inlet, and an outlet. A first stopcock is disposed within the first inlet and a second stopcock is disposed within the outlet. Solid phase material is prevented from escaping the reaction vessel via the outlet while allowing fluid to flow through the outlet by a frit. In one embodiment, the reaction vessel includes an outer hollow shell surrounding the vessel to allow cooling or heating fluid to flow therein for controlling the temperature in the interior reaction volume. The solid phase synthesis reaction vessel provides for the independent opening and closing of each of the inlets and outlet to provide for various ways of draining the vessel, varying the conditions within the vessel, and/or adding additional materials into the vessel.

SYSTEM AND METHOD FOR HIGH THROUGHPUT SCREENING OF DROPLETS

A system and method for high throughput screening of fluid samples. A reduced pressure is applied, via an injection valve, to a sample aspiration tube. A first fluid and a second fluid are alternatively aspired, via the sample aspiration tube, the first fluid for filling a sample loop with samples, the second fluid for flushing the sample aspiration tube. Excess fluid aspirated from the first fluid source and all fluid aspirated from the second fluid source is captured in an inline trap.

SYSTEM AND METHOD FOR HIGH THROUGHPUT SCREENING OF DROPLETS

DEVICE AND METHOD FOR THE TRANSFER OF LIQUID SAMPLES

Method and system for the detection of cardiac risk factors

Process and device for the simultaneous synthesis of several polypeptides

Polymer synthesizer

Method and apparatus for use in solid-phase physical, chemical, biological and biochemical techniques

SYSTEM AND METHOD FOR HIGH THROUGHPUT SCREENING OF DROPLETS

A system and method for high throughput screening of fluid samples. A reduced pressure is applied, via an injection valve, to a sample aspiration tube. A first fluid and a second fluid are alternatively aspired, via the sample aspiration tube, the first fluid for filling a sample loop with samples, the second fluid for flushing the sample aspiration tube. Excess fluid aspirated from the first fluid source and all fluid aspirated from the second fluid source is captured in an inline trap.

A DEVICE FOR THE ADDITION OF A COMPOUND OR COMPOUND MIXTURE TO ANOTHER UNDER VARIOUS REACTION CONDITIONS PARALLEL OR OTHERWISE

A device for the addition of a compound or compound mixture to another under various reaction conditions, parallel or otherwise is disclosed. The method and apparatus offer significant advantages over conventional experimental reactors, and is especially useful for synthesizing and screening large numbers of compounds. The device generally consists of a body (10) containing one or more recessed areas (14) surrounding one or more hollow pistons (18) mounted in barrels (30) covered by a top-plate (24). The recessed areas are filled with compounds. The hollow pistons are filled with other compounds. Using gas pressure the pistons move. This movement results in the addition of the compounds contained in the recessed areas to the compounds contained in the pistons. Modifications as found for conventional experimental reactors can also be incorporated in the apparatus.

MICROFLUIDIC CHIP FOR SYNTHESIZING RADIOACTIVELY LABELED MOLECULES SUITABLE FOR HUMAN IMAGING WITH POSITRON EMISSION TOMOGRAPHY

The invention concerns automated, inegrated microfluidic devices comprising a chemical reaction chip for performing reactions, such as the preparation of radiopharmaceutical compounds. The chip comprises an integrated microscale column and is configured for liquid flow from the column to at least one flow channel. The fluid flow is controlled by at least two on-chip valves. These double valves are configured consecutively and within proximity of 300 micrometer from each other. The reaction chamber is substantially "coinshaped", e.g. a reaction cylinder, preferably having a height diameter to height ratio greater than 3. Curved inlet and outlet channels ensure en effective elution.

METHOD FOR ISOLATION OF INDEPENDENT, PARALLEL CHEMICAL MICRO-REACTIONS USING A POROUS FILTER

The present invention relates to the field of fluid dynamics. More specifically, this invention relates to methods and apparatus for conducting densely packed, independent chemical reactions in parallel in a substantially two-dimensional array. Accordingly, this invention also focuses on the use of this array for applications such as DNA sequencing, most preferably pyrosequencing, and DNA amplification.

MICROFLUIDIC SELECTION OF LIBRARY ELEMENTS

Disclosed herein is a system comprising a chip; a flow channel disposed in the chip; the flow channel being in communication with an entry port and an exit port; the flow channel being operative to permit the flow of a library from the entry port to the exit port; a substrate; the substrate being disposed upon the chip; the substrate being operative to act as an upper wall for the flow channel; and a receptor; the receptor being disposed on the substrate; the receptor being operative to interact with a component from the library.

PROCESS AND DEVICE FOR THE SIMULTANEOUS SYNTHESIS OF SEVERAL POLYPEPTIDES

Process and device for the fully automatic simultaneous synthesis of several polypeptides, in which up to 48 different polypeptides may be synthesided in an automatic pipette by the solid-phase method of synthesis. The device has individual reaction vessels for the synthesis of the individual polypeptides which are brought to form one unit by a holding device. The simultaneous extraction of the fluids from the reaction vessels after reaction or washing process takes place via the holding device.

APPARATUS AND METHOD FOR POLYMER SYNTHESIS USING ARRAYS

Apparatus for liquid sample handling

Apparatus and method for combinatorial chemistry synthesis

Method and system for the detection of cardiac risk factors

Method for high throughput chemical screening

SPIRATING DEVICE CAPABLE OF SEPARATING FINE SOLID PARTICLES FROM A LIQUID

Dispositif de séparation des constituants liquides et solides d'un milieu réactionnel chimique (1) comprenant une aiguille filtrante (12) métallique pourvue de trous latéraux (5) permettant de passer à travers des membranes de type polymérique ou caoutchouteux (3) sans que celles-ci perdent leur étanchéité aux gaz (2), une source aspirante (7) capable d'aspirer un liquide hors d'une enceinte close (8), un canal d'évacuation du liquide (6),et un embout (4). FIGURE 1 <IMG> Device for separating the liquid and solid constituents of a chemical reaction medium (1) comprising a metal filter needle (12) provided with lateral holes (5) making it possible to pass through membranes of the polymeric or rubbery type (3) without those -these lose their gas tightness (2), a suction source (7) capable of sucking a liquid out of a closed enclosure (8), a liquid evacuation channel (6), and a nozzle (4) . FIGURE 1 <IMG>

APPARATUS AND METHOD FOR POLYMER SYNTHESIS USING ARRAYS

A polymer synthesis method and apparatus (20) is disclosed for building a polymer chain including a head assembly (21) having nozzles (22) with each nozzle coupled to a reservoir (23) of liquid reagent (24), and a base assembly (25) having reaction wells (26). A gas inlet (70) into the common chamber (31), upstream from the nozzles (22), and a gas outlet (71) out of the common chamber (31), downstream from the nozzles (22), sweeps the common chamber (31) of toxic fumes emitted by the reagents. A pressure regulating device (82) is provided for controlling a pressure differential, between pressure exerted on the reaction well (26) and pressure exerted on an exit (80) of the orifice (74), such that upon the pressure differential exceeding a predetermined amount, the reagent solution (76) is expelled.

A SOLID PHASE SYNTHESIS REACTION VESSEL AND METHOD OF USING THE SAME

A solid phase synthesis reaction vessel (1) includes a vessel (1) with a first inlet (3), a second inlet (5), and an outlet (7). A first stopcock (11) is disposed within the first inlet (3) and a second stopcock (13) is disposed within the outlet (7). Solid phase material is prevented from escaping the reaction vessel (1) via the outlet (7) while allowing fluid to flow through the outlet by a frit (9). In one embodiment, the reaction vessel (1) includes an outer hollow shell (31, 34) surrounding the vessel (1) to allow cooling or heating fluid to flow therein for controlling the temperature in the interior reaction volume (15). The solid phase synthesis reaction vessel (1) provides for the independent opening and closing of each of the inlets (3) and outlet (7) to provide for various ways of draining the vessel (1), varying the conditions within the vessel (1), and/or adding additional materials into the vessel (1).

APPARATUS FOR LIQUID SAMPLE HANDLING

There is provided according to the invention an apparatus for liquid sample handling which comprises: (a) a plurality of hollow capillaries (1), each being open at both ends and of defined internal volume, wherein on contact of an open end of a capillary (1) with a sample, said defined volume of sample is drawn up into the capillary (1) by capillary action; (b) a housing (2) which retains the capillaries in their desired orientation; and (c) means to effect sample removal from the capillaries; and uses thereof and processes employing said apparatus.

DEVICE FOR SURFACE FUNCTIONALIZATION AND DETECTION

A fluidic device (100) is described for locally coating an inner surface of a fluidic channel. The fluidic device (100) comprises a first (101), a second (102) and a third (103) fluidic channel intersecting at a common junction (105). The first fluidic channel is connectable to a coating fluid reservoir and the third fluidic channel is connectable to a sample fluid reservoir. The fluidic device (100) further comprises a fluid control means (111) configured for creating a fluidic flow path for a coating fluid at the common junction (105) such that, when coating, a coating fluid propagates from the first (101) to the second (102) fluidic channel via the common junction (105) without propagating into the third (103) fluidic channel. A corresponding method for coating and for sensing also has been disclosed.

ASPIRING DEVICE ALLOWING TO DISSOCIATE FROM the FINE SOLID PARTICLES Of WITH a LIQUID

DEVICE Of INJECTION PARALLELISEE AND SYNCHRONISEE FOR DIFFERENT INJECTIONS SEQUENTIELLESDE REACTIVE

COMPUTER-CONTROLLED, MODULE TYPE, MULTIPURPOSE SYNTHESIZER

The present invention provides a computer-controlled, module type, multipurpose synthesizer which contains a liquid flow control means by which the flow of reactants and solvents into the liquid flow control means is controlled by negative pressure and the flow of reactants and solvents out of the liquid flow control means is controlled by positive pressure. This apparatus also contains a directional flow valve means for controlling the direction of flow of reactants and solvents, which allows reactants and solvents to be rapidly passed from the liquid flow control means through the reaction chamber, continuously, rather than being discarded after each pass. This apparatus may also be equipped with a recycling system by which reactants and solvents can be used repeatedly, which can dramatically reduce the amount of expensive monomers usually required. Moreover, the present apparatus may be connected to a fraction collector to measure the progress of synthesis.

METHOD FOR ISOLATION OF INDEPENDENT, PARALLEL CHEMICAL MICRO-REACTIONS USING A POROUS FILTER

The present invention relates to methods and apparatus for conducting densely packed, independent chemical reactions in parallel in a substantially two-dimensional array. Accordingly, this invention also focuses on the use of this array for applications such as DNA sequencing, most preferably pyrosequencing, and DNA amplification.

Microfabricated flowthrough porous apparatus for discrete detection binding reactions

An improved microfabricated apparatus for conducting a multiplicity of individual and simultaneous binding reactions is described. The apparatus comprises a substrate on which are located discrete and isolated sites for binding reactions. The apparatus is characterized by discrete and isolated regions that extend through said substrate and terminate on a second surface thereof such that when a test sample is allowed to the substrate, it is capable of penetrating through each such region during the course of said binding reaction. The apparatus is especially useful for sequencing by hybridization of DNA molecules.

Parallel loading of arrays

A solid phase synthesis reaction vessel and method of using the same

HIGH THROUGHPUT MECHANICAL ALLOYING AND SCREENING

The invention provides an apparatus and methods for conducting ball milling operations in a high throughput manner. Also provided are devices and methods for high throughput screening of alloys and other materials to identify those that have desired gas storage properties.

AUTOMATED OLIGOMER SYNTHESIS

L'invention concerne des systèmes comprenant un appareil et des procédés de synthèse automatisée d'oligomères.

MICROFLUIDIC DEVICES WITH CHEMICAL REACTION CIRCUITS

New microfluidic devices, useful for carrying out chemical reactions, are provided. The devices are adapted for on-chip solvent exchange, chemical processes requiring multiple chemical reactions, and rapid concentration of reagents.

DEVICE AND METHOD FOR THE TRANSFER OF LIQUID SAMPLES

The invention relates to a transfer device (1) for removing liquid samples (2) from containers (3) and for introducing said liquid samples (2) into chambers (5), arranged below said containers. The device (1) has an individual chamber (5', 5'') for each of the containers (3, 3'). Said transfer devices (1) are characterised in having containing means (4), comprising a single individual restriction opening (10) for each container (3, 3'), or for each chamber (5, 5', 5''), which defines the flow of fluids for introducing into the containers (3, 3') or for removal from the chambers (5, 5', 5''). The invention further relates to a method for removing liquid samples (2) form containers (3) and for introducing said liquid samples (2) into chambers (5) arranged beneath said containers with such a transfer device (1). The device (1) can be used for the individual immobilisation of liquid samples (2) on MALDI-MS targets (21), or for the collection of liquid samples in individual collection chambers (12).

Apparatus for automated synthesis

Disclosed is an apparatus for automated synthesis containing a shaker deck with a plurality of reaction blocks containing a plurality of reaction wells, an arm containing an array of extending units capable of actuating physical steps along a plurality of axes of each reaction block, each reaction block being situated on a turntable, each turntable being further capable of rotating the reaction block along a central axis. Also disclosed are methods of preparing a peptide, oligonucleotide an small molecules using the apparatus.

DEVICE FOR PRODUCING POLY(METH)ACRYLATE IN POWDER FORM

The invention relates to an apparatus for producing pulverulent poly(meth)acrylate, comprising a reactor for droplet polymerization having an apparatus for dropletization of a monomer solution for the preparation of the poly(meth)acrylate having holes through which the monomer solution is introduced, an addition point for a gas above the apparatus for dropletization, at least one gas withdrawal point on the circumference of the reactor, a fluidized bed and an apparatus for product discharge from the fluidized bed. The apparatus for product discharge comprises a discharge apparatus, with a backup segment () disposed above the discharge apparatus. 1. An apparatus for producing pulverulent poly(meth)acrylate , comprising a{'b': 1', '5', '13', '5', '19', '1', '11', '33', '11', '33', '49', '39', '49, 'reactor () for droplet polymerization having an apparatus () for dropletization of a monomer solution for the preparation of the poly(meth)acrylate having holes through which the monomer solution is introduced, an addition point () for a gas above the apparatus () for dropletization, at least one gas withdrawal point () on the circumference of the reactor (), a fluidized bed () and an apparatus () for product discharge from the fluidized bed (), wherein the apparatus () for product discharge comprises a discharge apparatus (), with a backup segment () disposed above the discharge apparatus ().'}2394347. The apparatus according to claim 1 , wherein the backup segment () has an upper sensor for detecting a maximum fill level () and a lower sensor for detecting a minimum fill level ().3414345334733. The apparatus according to claim 2 , wherein a closed-loop fill level control system is included claim 2 , in which the upper sensor detects a first upper fill level () and claim 2 , when the latter is attained claim 2 , the feed is reduced claim 2 , and detects a second upper fill level () and claim 2 , when the latter is attained claim 2 , the feed is closed claim 2 , and the ...

Device for Surface Functionalization and Detection

A fluidic device (100) is described for locally coating an inner surface of a fluidic channel. The fluidic device (100) comprises a first (101), a second (102) and a third (103) fluidic channel intersecting at a common junction (105). The first fluidic channel is connectable to a coating fluid reservoir and the third fluidic channel is connectable to a sample fluid reservoir. The fluidic device (100) further comprises a fluid control means (111) configured for creating a fluidic flow path for a coating fluid at the common junction (105) such that, when coating, a coating fluid propagates from the first (101) to the second (102) fluidic channel via the common junction (105) without propagating into the third (103) fluidic channel. A corresponding method for coating and for sensing also has been disclosed.

MULTIPLE CAPILLARY DEVICE AND METHOD FOR SYNTHESIS AND DISPENSING

PROCESS AND DEVICE FOR THE SIMULTANEOUS SYNTHESIS OF SEVERAL POLYPEPTIDES

GERÄT UND VERFAHREN ZUR POLYMERSYNTHESE MIT GERÜSTEN

Microarray-Vorrichtung mit mikroporöser Membrane und einer Inkubationskammernanordnung

MULTI-CHANNEL REAGENT DISPENSING APPARATUS AND METHOD

CHEMICAL REACTION MONITOR

An apparatus for parallel monitoring of a plurality of chemical reactions, each chemical reaction occurring within a respective one of a plurality of wells. The apparatus includes a lighting device for illuminating the plurality of wells, the lighting device having an light emitting diode (LED) array, a camera device configured to obtain images of the plurality of wells and saving the images to an image storage location, the camera device having a charge couple device (CCD) capable of imaging the plurality of wells simultaneously, a viewing program for viewing one or more of the saved images, and an analyzing program for opening each saved image and geometrically registering each saved image in order to determine a specific value corresponding to each one of the chemical reactions at the time the image was obtained, the analyzing program saving the specific values to an analysis results database. A method of utilizing the apparatus is also disclosed.

CHEMICAL REACTION MONITOR

An apparatus for parallel monitoring of a plurality of chemical reactions, each chemical reaction occurring within a respective one of a plurality of wells. The apparatus includes a lighting device for illuminating the plurali ty of wells, the lighting device having an light emitting diode (LED) array, a camera device configured to obtain images of the plurality of wells and savi ng the images to an image storage location, the camera device having a charge couple device (CCD) capable of imaging the plurality of wells simultaneously , a viewing program for viewing one or more of the saved images, and an analyzing program for opening each saved image and geometrically registering each saved image in order to determine a specific value corresponding to eac h one of the chemical reactions at the time the image was obtained, the analyzing program saving the specific values to an analysis results database . A method of utilizing the apparatus is also disclosed.

DEVICE FOR POWDER METERING FOR CHEMICAL PRODUCTION PROCESSES UNDER CLEAN-ROOM CONDITIONS, USE THEREOF AND METERED ADDITION METHOD

Device for metering powder, in particular in clean-rooms, which includes a vessel containing powder and a sealing head with a septum for the vessel, wherein the sealing head is connectable powder-tight with the vessel and the septum powder-tight with the sealing head and the device further includes a vessel holder, which serves to hold the sealing head of the vessel, and the vessel with its opening points downwards, so that the powder can flow out of the vessel, wherein a gap is provided between the sealing head and a holding bowl of the vessel holder, in which a gas flow between the holding bowl and the sealing head can be created. The invention also relates to a use of the device and a metered addition method. 1. A device for metering powder , which comprises a vessel containing powder and a sealing head with a septum for the vessel , wherein the sealing head is connectable powder-tight with the vessel and the septum with the sealing head , and the device further comprises a vessel holder , which serves to hold the sealing head of the vessel , and the vessel with its opening points downwards , so that the powder can flow out of the vessel , wherein a gap is present between the sealing head and a holding bowl of the vessel holder , in which a gas flow between the holding bowl and the sealing head can be created.2. The device according to claim 1 , wherein the cross-section of the gap between the holding bowl and the sealing head decreases in the direction of the septum claim 1 , so that the flow rate of the gas increases in the direction of the septum.3. The device according to one of claim 1 , wherein the gap between the holding bowl and the sealing head is shaped such that the flow rate is maximal on the septum and the flow is guided against the septum in order to remove powder particles from the septum and the sealing head.4. The device according to claim 1 , wherein the sealing head has a septum adapter and a septum cap with an opening claim 1 , wherein the ...

Well plate and suction device provided with well plate

A well plate is formed with a well for holding a subject to be sucked by a suction nozzle on an inner bottom part and storing liquid and, a clearance forming member for forming a clearance to allow the liquid to flow in a state where a tip part of the suction nozzle is inserted into and held in contact with the well is provided in the well. According to the present invention, the clearance enabling the liquid to flow is formed even in the state where the tip part of the suction nozzle is inserted into and held in contact with the well in sucking the subject held in the well by the suction nozzle. The suction nozzle can suck the liquid around through the clearance and the subject held in the well is efficiently sucked through the suction port along the flow of the sucked liquid.

NUCLEIC ACID INTEGRATED DETECTION METHOD AND DETECTION REAGENT TUBE

A nucleic acid integrated detection method and detection reagent tube are provided, separating a lysis solution, a cleaning solution and a reaction solution in a detection reagent tube by providing a plurality of separation plugs in an over-under arrangement and disposing a hydrophobic layer in liquid or solid phase on each separation plug; adding a sample into the lysis solution; extracting nucleic acid in the sample using magnetic nanobeads; and then driving the magnetic nanobeads carrying the nucleic acid to sequentially pass through each hydrophobic layer along a magnetic bead channel and into the cleaning solution and the reaction solution to realize a cleaning and amplification for the nucleic acid, and finally, detecting the nucleic acid of the sample by an external device using an optical detection method, thus realizing a plurality of steps of nucleic acid extraction, cleaning and amplification reactions in the same detection reagent tube. 1. A nucleic acid integrated detection method , the method comprising:separating a lysis solution, a cleaning solution and a reaction solution in a detection reagent tube by providing a plurality of separation plugs arranged one above one another and disposing a hydrophobic layer in a liquid or a solid phase at each separation plug;adding a sample into the lysis solution for mixing and lysis;extracting a nucleic acid in the sample using magnetic nanobeads; and then driving, by an external magnet, the magnetic nanobeads carrying the nucleic acid to sequentially pass through each hydrophobic layer along a magnetic bead channel in an inner wall of the detection reagent tube and into the cleaning solution and the reaction solution to realize a cleaning and amplification for the nucleic acid, wherein a biological agent required in the reaction solution is stored in a separation plug above the reaction solution; anddetecting the nucleic acid of the sample by an external device using an optical detection method, thus realizing a ...

Microarray processing apparatus, well plate for microarray processing apparatus, microarray holder, and microarray washing method

Provided is a microarray processing apparatus which is capable of sufficiently washing a microarray. The microarray processing apparatus ( 30 ) includes a well plate ( 38 ) in which one or two or more wells ( 40 ) are formed, each well ( 40 ) accommodating a microarray ( 1 ), and a suction nozzle ( 46 ) that suctions a liquid from the well. The well has a concave portion whose upper end is opened, which has a depth equal to or greater than a height of the microarray, and into which a front end of the suction nozzle can be inserted up to a height position of a lower end of the microarray accommodated in the well. The suction nozzle can relatively descend in the well until the front end of the suction nozzle is located at the height position of the lower end of the microarray accommodated in the well.

Microreactor array platform

A microreactor array platform and method for sealing a reagent in microreactors of an array of microreactors are provided. The microreactor array platform includes an array of microreactors, and a sealing film having a first surface and an opposite second surface, the sealing film configured to movably seal the array of microreactors. The microreactor array platform also includes an injector for delivering a reagent into the array of microreactors via a fluid path between the array and the second surface of the sealing film, and an applicator for directing a sealing liquid against the first surface of the sealing film. The microreactor array platform further includes a system for creating a pressure differential between the reagent in the injector and a space between the array of microreactors and the second surface of the sealing film.

MICROARRAY PROCESSING APPARATUS, WELL PLATE FOR MICROARRAY PROCESSING APPARATUS, MICROARRAY HOLDER, AND MICROARRAY WASHING METHOD

Provided is a microarray processing apparatus which is capable of sufficiently washing a microarray. The microarray processing apparatus () includes a well plate () in which one or two or more wells () are formed, each well () accommodating a microarray (), and a suction nozzle () that suctions a liquid from the well. The well has a concave portion whose upper end is opened, which has a depth equal to or greater than a height of the microarray, and into which a front end of the suction nozzle can be inserted up to a height position of a lower end of the microarray accommodated in the well. The suction nozzle can relatively descend in the well until the front end of the suction nozzle is located at the height position of the lower end of the microarray accommodated in the well. 1. A well plate comprising:a plate; andone or more wells formed in the plate, each being capable of accommodating a microarray and having a concave portion formed at an inner wall of each well,wherein the concave portion comprises an opened upper end and extends along a depth direction of each well from a bottom surface of each well to the opened upper end, a length of the concave portion along the depth direction of each well is equal to or greater than a height of the microarray, and front end of a suction nozzle that suctions a liquid from each well can be inserted into the concave portion up to a height position of a lower end of the microarray accommodated in each well.2. The well plate according to claim 1 ,wherein the concave portion of each well has a shape into which the front end of the suction nozzle can be inserted up to a position lower than the height position of the lower end of the microarray accommodated in each well.3. The well plate according to claim 1 ,wherein the concave portion of each well has a shape into which a front end of an injection nozzle through which a liquid is injected into each well can be inserted up to a position lower than a height position of an upper ...

DEVICE FOR POWDER METERING FOR CHEMICAL PRODUCTION PROCESSES UNDER CLEAN-ROOM CONDITIONS, USE THEREOF AND METERED ADDITION METHOD

Device for metering powder, in particular in clean-rooms, which includes a vessel containing powder and a sealing head with a septum for the vessel, wherein the sealing head is connectable powder-tight with the vessel and the septum powder-tight with the sealing head and the device further includes a vessel holder, which serves to hold the sealing head of the vessel, and the vessel with its opening points downwards, so that the powder can flow out of the vessel, wherein a gap is provided between the sealing head and a holding bowl of the vessel holder, in which a gas flow between the holding bowl and the sealing head can be created. The invention also relates to a use of the device and a metered addition method. 1. Device for metering powder , which comprises a vessel containing powder and a sealing head with a septum for the vessel , and the vessel points downwards with its opening , so that the powder can flow out of the vessel , and the device further has a vessel holder , which serves to hold the sealing head of the vessel , wherein the vessel holder has a cannula for piercing the septum and a gas for fluidizing the downward-flowing powder is fed to the cannula , and further a metering system is provided , which is based on the filling of a volume in a connecting hose separable by valves , wherein the bulk density of the powder in the connecting hose is kept essentially constant by a gas flow varying with time during the fluidization.2. Device according to claim 1 , wherein the gas volume of the fluidization amounts to between 0.3 and 3.0 times claim 1 , preferably between 0.5 and 2.0 times the separable volume in the connecting hose and/or the gas flow is greatest at the end of the fluidization.3. Device according to claim 1 , wherein the sealing head is connectable powder-tight with the vessel and the septum with the sealing head claim 1 , and wherein a gap is present between the sealing head and a holding bowl of the vessel holder claim 1 , in which a gas ...

DEVICE FOR PARALLEL OLIGOMER SYNTHESIS, METHOD OF PARALLEL OLIGOMER SYNTHESIS AND USE THEREOF

A device for parallel oligomer synthesis having a centrifuge with a plurality of reactor holders configured to retain reactors at an angle and a plurality of siphon based outflow holders are disclosed. A method of parallel solid-based peptide synthesis following the timing protocol of the device and a use of the device for parallel oligomer synthesis are also disclosed. 1. A device for parallel oligomer synthesis , the device comprising:a centrifuge, the centrifuge comprisinga plurality of reactor holders, the reactor holders being configured to retain reactors at an angle, anda plurality of holders for siphon based outflows,a plurality of reactors, the reactors having upper ends and lower ends, the lower ends comprising nozzles, the reactors being positioned at an angle in the reactor holders,a plurality of siphon based outflows, the siphon based outflows having first ends and second ends, the first ends being connected to the nozzles of the lower ends of reactors, and the siphon based outflows being configured to work on a siphoning principle,a distribution rotor, the distribution rotor being provided with pre-activation compartments at its outer circumference, the pre-activation compartments being provided with grooves directed towards the outer edge of the distribution rotor, and configured to enable the transfer of contents from the pre-activation compartments of the distribution rotor to the reactors upon rotation of the distribution rotor,a lifting device, the lifting device being configured to enable the distribution rotor to move in vertical direction between an upper position and a lower position,a positioning device, the positioning device being configured to enable a rotation of the distribution rotor in the upper position independently from the centrifuge,a driving motor, the driving motor, the centrifuge and the distribution rotor having the same common axis, the driving motor being configured to enable a synchronized rotation of the centrifuge and the ...

Microfluidic device for analyzing nucleic acids and/or proteins, methods of preparation and uses thereof

The invention relates to a microfluidic device for nucleic acid and/or protein analysis, wherein said device comprises a capillary on the inner surface of which is attached an array of at least two reagents. Also encompassed by the invention is such a capillary. In addition, the invention concerns methods for attaching at least two reagents on the inner surface of a capillary, as well as methods using a microfluidic device and enabling a multiplex analysis of nucleic acids and/or proteins to be performed.

Method and system for the detection of cardiac risk factors

A system for the rapid characterization of multi-cardiovascular risk factor analyte fluids, in one embodiment, includes a light source, a sensor array, and a detector. The sensor array is formed from a supporting member, in which a plurality of cavities may be formed. A series of chemically sensitive particles, in one embodiment, are positioned within the cavities. The particles may produce a signal when a receptor, coupled to the particle, interacts with the cardiovascular risk factor analyte and the particle-analyte complex is visualized using a visualization reagent. Using pattern recognition techniques, the analytes within a multi-analyte fluid may be characterized. In an embodiment, each cavity of the plurality of cavities is designed to capture and contain a specific size particle. Flexible projections may be positioned over each of the cavities to provide retention of the particles in the cavities.

Systems for particle manipulation

Systems, including methods and apparatus, for particle manipulation to perform assays.

METHOD AND APPARATUS FOR CARRYING OUT BIOCHEMICAL REACTIONS

PCT No. PCT/SE91/00523 Sec. 371 Date Mar. 19, 1992 Sec. 102(e) Date Mar. 19, 1992 PCT Filed Aug. 7, 1991 PCT Pub. No. WO92/02303 PCT Pub. Date Feb. 20, 1992.An apparatus for carrying out biochemical reactions in microtiter plates includes a device (42, 43) for receiving and holding microtiter plate (44) to be processed, the microtiter plate being provided with a plurality of wells and each well having a bottom outlet, and a movably mounted device (47) for the application of gas pressure to the wells of the microtiter plate (44), the pressure device including a sealing collar (48) and being movable between a position where it leaves the upper part of the microtiter plate accessible, and a position where it sealingly contacts the microtiter plate through the sealing collar (48) so that a gas pressure may be applied to the inlets of the microtiter plate wells for pressing out liquid present therein through the bottom outlet of each well.

Method and system for the detection of cardiac risk factors

A system for the rapid characterization of multi-cardiovascular risk factor analyte fluids, in one embodiment, includes a light source, a sensor array, and a detector. The sensor array is formed from a supporting member, in which a plurality of cavities may be formed. A series of chemically sensitive particles, in one embodiment, are positioned within the cavities. The particles may produce a signal when a receptor, coupled to the particle, interacts with the cardiovascular risk factor analyte and the particle-analyte complex is visualized using a visualization reagent. Using pattern recognition techniques, the analytes within a multi-analyte fluid may be characterized. In an embodiment, each cavity of the plurality of cavities is designed to capture and contain a specific size particle. Flexible projections may be positioned over each of the cavities to provide retention of the particles in the cavities.

聚合物回收与洗涤方法

本发明涉及聚合物材料的回收和洗涤方法，特别是来自高通量反应容器的聚合物样品的回收和洗涤的自动方法，所述方法包含：(i)使用包含C5到C8链烷烃的漂洗液将聚合物样品从高通量反应容器漂洗到过滤容器中，在所述过滤容器中提供包含聚合物样品、漂洗液和氧合物的混合物，(ii)通过过滤，在所述过滤容器中将漂洗液和氧合物从聚合物样品中除去，和(iii)随后在所述过滤容器中洗涤所述的聚合物样品以除去任何残留的包含C5到C8链烷烃的漂洗液。

一种核酸一体化检测方法及检测试剂管

本发明公开了一种核酸一体化检测方法及检测试剂管，通过在主管内设置多个呈上下布置的分隔塞，并在每个分隔塞上设置液相或固相的疏水层，从而将检测试剂管内的裂解液、清洗液和反应液进行隔离；将样品加入裂解液中进行混匀和裂解,并利用纳米磁珠对样品中的核酸进行提取，然后利用外部磁性体带动纳米磁珠携带核酸沿着检测试剂管内壁上的磁珠通道依次穿过各个疏水层进入清洗液和反应液，实现核酸的清洗和扩增，其中，反应液所需的生物试剂储藏在反应液上方的分隔塞内，最后外部设备通过光学检测实现对样品核酸的检测，从而实现在同一个检测试剂管内进行核酸提取、清洗和扩增反应多个步骤。本发明具有能够减少检测误差和降低操作难度的特点。

Synthesis of oligomers in arrays

Systems, including apparatus and methods, for synthesis of oligomers in arrays.

Well plate and suction device provided with well plate

A well plate is formed with a well for holding a subject to be sucked by a suction nozzle on an inner bottom part and storing liquid and, a clearance forming member for forming a clearance to allow the liquid to flow in a state where a tip part of the suction nozzle is inserted into and held in contact with the well is provided in the well. According to the present invention, the clearance enabling the liquid to flow is formed even in the state where the tip part of the suction nozzle is inserted into and held in contact with the well in sucking the subject held in the well by the suction nozzle. The suction nozzle can suck the liquid around through the clearance and the subject held in the well is efficiently sucked through the suction port along the flow of the sucked liquid.

Bead trapping device

A bead trapping device for trapping beads. The device is formed from a substrate, posts extending from the substrate; the posts being patterned to form cavities between the posts, the cavities having a diameter between 0.5 μm and 200 μm; and means for loading beads onto the substrate. A method of trapping microscopic beads for analysis, the method comprising the steps of contacting a fluid containing beads with a substrate having posts extending from the substrate to form bead trapping cavities, the bead trapping cavities having a diameter between 0.5 μm and 200 μm; and loading the beads into the bead trapping cavities.

Method and apparatus for printing a pattern of different reagent fluids on a porous substrate while applying a pressure drop and porous substrate printed with distinct dots having an enhanced depth of penetration

The invention relates to a method of printing a pattern of distinct dots (40, 62) of different reagent fluids (23) on a porous substrate (8) while a pressure drop is applied across the thickness of the substrate from the printing side (36) to the opposite side (34). The printed porous substrate (8) is used for a chemical assays. The invention also relates to a porous substrate (8) obtainable by said method, wherein the distinct dots (40, 62) of fluid have an enhanced penetration depth. A printing apparatus for carrying out the method according to the invention is also included in the invention. The invention leads to a reduction in the loss of reagent fluid in the printing process and an enhancement of the sensitivity of the substrate.

Method and device to remove fluid and vapor

The present inventioti relates to a method and device for the removal of tluids and vapors in containment, fronl aiijacent surtaces including supporting flat surfaces and substrates, such as protein spots printed in arravs atid micro-array fot-mats. This method and device removes fluids and vapors under controlled and repeatable cunditions, enabling uuifortn phases for degree of drying, incurred bv objects, specimetl and structures. including tissucs, adsorbed particles and biological sttbstrates, while reducing and preventing impact of ineniscus phase surface tension forces.

Microfluidic chip capable of synthesizing radioactively labeled molecules on a scale suitable for human imaging with positron emission tomography

본 발명은 자동화된 통합 마이크로유체 장치로서 화학 반응을 위한 화학 반응 칩, 및 칩이 통합되고, 컬럼으로부터 최소한 하나의 유동 채널에 액체 유동을 위하여 구성된 미소규모의 컬럼을 포함하며, 여기서 컬럼으로의 유체 유동은 온-칩 밸브에 의하여 조절되며, 및 마이크로유체 장치 내의 유체 유동을 조절하기 위한 최소한 2개의 온-칩 밸브를 포함한다. 마이크로유체 칩, 양전자 방출 단층촬영, 인체 영상화, 방사성 표지 분자

Microfluidic chip capable of synthesizing radioactively labeled molecules on a scale suitable for human imaging with positron emission tomography

본 발명은 자동화된 통합 마이크로유체 장치로서 화학 반응을 위한 화학 반응 칩, 및 칩이 통합되고, 컬럼으로부터 최소한 하나의 유동 채널에 액체 유동을 위하여 구성된 미소규모의 컬럼을 포함하며, 여기서 컬럼으로의 유체 유동은 온-칩 밸브에 의하여 조절되며, 및 마이크로유체 장치 내의 유체 유동을 조절하기 위한 최소한 2개의 온-칩 밸브를 포함한다. The present invention is an automated integrated microfluidic device comprising a chemical reaction chip for a chemical reaction, and a microscale column in which the chip is integrated and configured for liquid flow from the column to at least one flow channel, wherein the fluid to the column The flow is controlled by an on-chip valve and includes at least two on-chip valves for regulating fluid flow in the microfluidic device. 마이크로유체 칩, 양전자 방출 단층촬영, 인체 영상화, 방사성 표지 분자 Microfluidic Chips, Positron Emission Tomography, Human Imaging, Radiolabelled Molecules

Combinatorial chemical synthesis

조합 합성 방법은 복수의 수집기를 갖춘 장치를 이용하여 재료적으로 상이한 특성을 갖는 복수의 조성물을 얻는다. 제1 정량의 생성 조성물을 형성하도록 제1 정량의 유체 반응물을 반응시킨다. 제1 정량의 생성 조성물의 수집이 완료된 후에, 상기 제1 정량의 생성 조성물과 재료적으로 상이한 제2 정량의 생성 조성물을 형성하도록 제2 정량의 유체 반응물을 반응시킨다. 장치는 반응물 공급원(120)에 연결된 노즐(130)과 복수의 수집기(236)를 구비한다. 노즐과 복수의 수집기는, 수집기가 노즐로부터 방출되는 유체 스트림(fluid stream)을 수용하게 선택적으로 배치될 수 있도록 서로에 대해 이동한다. 복수의 생성 조성물은 다양한 용례에 대한 그들의 적합성을 결정하도록 평가될 수 있다. The combinatorial synthesis method uses a device equipped with a plurality of collectors to obtain a plurality of compositions having materially different properties. The first quantity of fluid reactant is reacted to form a first quantity of product composition. After collection of the first quantity of product composition is completed, the second quantity of fluid reactant is reacted to form a second amount of product composition that is materially different from the first amount of product composition. The apparatus has a nozzle 130 and a plurality of collectors 236 connected to the reactant source 120. The nozzle and the plurality of collectors move relative to each other such that the collector can be selectively arranged to receive a fluid stream exiting the nozzle. Multiple product compositions can be evaluated to determine their suitability for various applications.

New integrated microarray analysis

本発明は、以下の段階を含む、核酸分子を含む試料中に存在する検体核酸を同定するための方法に関する：(a) 多孔性基体を準備する段階であって、上記基体は複数のマイクロチャンネルを含み、上記マイクロチャンネルは、その上に上記試料中に存在する検体に結合することができる標的分子が固定されており、上記チャンネルはさらに、検体増幅成分、レポーターシステム；および上記試料；が提供され；(b) 上記試料中に存在する検体核酸分子を増幅する段階；および(c) 段階(b)で得られた増幅された検体核酸、上記基体のチャンネル上に固定された上記標的分子、および上記レポーターシステムとの間で結合を生じさせる段階であって、上記レポーターシステムにより、上記標的分子と上記検体核酸との間で結合が生じたかどうかを検出することができる段階。本発明はさらに、上記方法の使用、さらには上記方法を実行するためのキットに関する。

Apparatus and method for combinatorial chemistry synthesis

In a first embodiment, this invention includes an integrated robot apparatus for performing combinatorial chemistry synthesis protocols and having interchangeable work-stations, robot arm tools, and reaction vessels and reaction vessel arrays. The work-stations and tools are specialized to perform tasks necessary for the synthesis in a plurality of the reaction vessels grouped in a plurality of the reaction vessel arrays. Preferably, these elements function interchangeably because they have standardized sizes and conformation. The work-stations and tools include those for fluid dispensing or aspirating from individual reaction vessels or from all the reaction vessels in an array simultaneously. The reaction vessels can include, alternatively, stackable, ball-sealed reaction vessels, microtitre-like reaction vessel arrays, arrays of independent reaction vessels, valve-sealed reaction vessels, septum-sealed reaction vessels, and syringe reaction vessels. In alternative embodiments, this invention includes these work-stations, tools, reaction vessels and reaction vessel arrays in various combinations or sub-combinations either for use in partially integrated robots or for manual or standalone use.

Microfluidic devices with chemical reaction circuits

New microfluidic devices, useful for carrying out chemical reactions, are provided. The devices are adapted for on-chip solvent exchange, chemical processes requiring multiple chemical reactions, and rapid concentration of reagents.

Microfluidic chip for synthesizing radioactively labeled molecules suitable for human imaging with positron emission tomography

The invention concerns automated, inegrated microfluidic devices comprising a chemical reaction chip for performing reactions, such as the preparation of radiopharmaceutical compounds. The chip comprises an integrated microscale column and is configured for liquid flow from the column to at least one flow channel. The fluid flow is controlled by at least two on-chip valves. These double valves are configured consecutively and within proximity of 300 micrometer from each other. The reaction chamber is substantially 'coinshaped', e.g. a reaction cylinder, preferably having a height diameter to height ratio greater than 3. Curved inlet and outlet channels ensure en effective elution.

Apparatus and method for polymer synthesis using arrays

A polymer synthesis method and apparatus (20) is disclosed for building a polymer chain including a head assembly (21) having nozzles (22) with each nozzle coupled to a reservoir (23) of liquid reagent (24), and a base assembly (25) having reaction wells (26). A gas inlet (70) into the common chamber (31), upstream from the nozzles (22), and a gas outlet (71) out of the common chamber (31), downstream from the nozzles (22), sweeps the common chamber (31) of toxic fumes emitted by the reagents. A pressure regulating device (82) is provided for controlling a pressure differential, between pressure exerted on the reaction well (26) and pressure exerted on an exit (80) of the orifice (74), such that upon the pressure differential exceeding a predetermined amount, the reagent solution (76) is expelled.

Microfluidic devices with chemical reaction circuits

New microfluidic devices, useful for carrying out chemical reactions, are provided. The devices are adapted for on-chip solvent exchange, chemical processes requiring multiple chemical reactions, and rapid concentration of reagents.

Device for surface functionalization and detection

描述一种用于局部涂覆流体通道的内表面的流体设备(100)。流体设备(100)包括在公共接合点(105)处相交的第一流体通道(101)、第二流体通道(102)和第三流体通道(103)。第一流体通道可连接到涂层流体贮存器，并且第三流体通道可连接到样本流体贮存器。流体设备(100)还包括流体控制装置(111)，该装置被配置用于在公共接合点(105)处产生用于涂层流体的流体流动路径，使得当涂覆时，涂层流体经由公共接合点(105)从第一流体通道(101)传播到第二流体通道(102)，而不传播到第三流体通道(103)中。已经公开了用于涂覆和感测的对应方法。

For chemical production processes for the device of powder metering, its purposes and metering adding method under clean-room condition

本发明涉及一种用于计量粉末(59)的装置、特别是在洁净室中用于计量粉末的装置，所述装置包括容纳粉末(59)的容器(56)和具有分隔件(115)的用于容器(56)的密封头(57)，其中，密封头(57)可与容器(56)粉末密封地连接，分隔件(115)可与密封头(57)粉末密封地连接，所述装置还包括容器保持器(58)，所述容器保持器用于保持容器(56)的密封头(57)，容器(56)以其开口(56a)指向下方，使得粉末(59)可以流出容器(56)，其中，在密封头(57)和容器保持器(58)的保持碗(131)之间存在间隙(200)，在所述间隙中，可以在保持碗(131)和密封头(57)之间产生气体流动。本发明还涉及所述装置的用途和计量添加方法。

Method for isolation of independent, parallel chemical micro-reactions using a porous filter

The present invention relates to the field of fluid dynamics. More specifically, this invention relates to methods and apparatus for conducting densely packed, independent chemical reactions in parallel in a substantially two-dimensional array. Accordingly, this invention also focuses on the use of this array for applications such as DNA sequencing, most preferably pyrosequencing, and DNA amplification.

Apparatus for liquid sample handling

Multiple capillary device and method for synthesis and dispensing

A system and method for synthesizing a peptide, a nucleic acid sequence, an oligonucleotide, a DNA sequence, an RNA sequence, or the like, inside an array of capillary tubes, is provided. The system can comprise an array of capillary tubes. Each of the capillary tubes in the array of capillary tubes can comprise a first end, a second end, an inner wall, and a sequence linker bonded to the inner wall. The system can comprise a pressure control source that can be in fluid communication with each of the first ends of the array of capillary tubes. The system can comprise a reagent container support, wherein the second end of each of the capillary tubes can be adapted to move towards and/or away from the reagent container support.

Method and system for the detection of cardiac risk factors

A system for the rapid characterization of multi-cardiovascular risk factor analyte fluids, in one embodiment, includes a light source, a sensor array, and a detector. The sensor array is formed from a supporting member, in which a plurality of cavities may be formed. A series of chemically sensitive particles, in one embodiment, are positioned within the cavities. The particles may produce a signal when a receptor, coupled to the particle, interacts with the cardiovascular risk factor analyte and the particle-analyte complex is visualized using a visualization reagent. Using pattern recognition techniques, the analytes within a multi-analyte fluid may be characterized. In an embodiment, each cavity of the plurality of cavities is designed to capture and contain a specific size particle. Flexible projections may be positioned over each of the cavities to provide retention of the particles in the cavities.

Nucleic acid integrated detection method and detection reagent tube

A nucleic acid integrated detection method and detection reagent tube are provided, separating a lysis solution, a cleaning solution and a reaction solution in a detection reagent tube by providing a plurality of separation plugs in an over-under arrangement and disposing a hydrophobic layer in liquid or solid phase on each separation plug; adding a sample into the lysis solution; extracting nucleic acid in the sample using magnetic nanobeads; and then driving the magnetic nanobeads carrying the nucleic acid to sequentially pass through each hydrophobic layer along a magnetic bead channel and into the cleaning solution and the reaction solution to realize a cleaning and amplification for the nucleic acid, and finally, detecting the nucleic acid of the sample by an external device using an optical detection method, thus realizing a plurality of steps of nucleic acid extraction, cleaning and amplification reactions in the same detection reagent tube.

Methods for reagent removal in flow chambers

Methods and apparatus are disclosed for synthesizing a plurality of compounds on the surface of supports. Biopolymer features are attached to the surfaces of the supports. The synthesis generally comprises a plurality of steps. The support is placed into a flow chamber, and a reagent is introduced into the flow chamber. The reagent is reactive with features on the surface of the support. During removal of the reagent from the flow chamber, the pressure in the chamber is maintained substantially atmospheric. In another embodiment the reagent is removed from the flow chamber under vacuum. In another embodiment the reagent is removed from the flow chamber by simultaneously venting and applying a vacuum to the flow chamber.

Chemical reaction monitor

An apparatus for parallel monitoring of a plurality of chemical reactions, each chemical reaction occurring within a respective one of a plurality of wells. The apparatus includes a lighting device for illuminating the plurality of wells, the lighting device having an light emitting diode (LED) array, a camera device configured to obtain images of the plurality of wells and saving the images to an image storage location, the camera device having a charge couple device (CCD) capable of imaging the plurality of wells simultaneously, a viewing program for viewing one or more of the saved images, and an analyzing program for opening each saved image and geometrically registering each saved image in order to determine a specific value corresponding to each one of the chemical reactions at the time the image was obtained, the analyzing program saving the specific values to an analysis results database. A method of utilizing the apparatus is also disclosed.

Method and apparatus for oligo peptide synthesis

An oligo peptide synthesis apparatus comprises a stationary apparatus part, which is stationary in relation to the frame and a movable apparatus part. The movable apparatus part comprises an oligo peptide synthesis reaction holder, which comprises a number of receptors for receiving a corresponding number of vessels and the receptors are located on a circle. The movable apparatus part comprises a revolveable holder, which has a disengageable coupling for connecting the revolveable holder to the oligo peptide synthesis reaction holder.

Small volume chambers

Apparatus and methods are disclosed for forming a reaction chamber having relatively small volumes. An apparatus comprises two elements and a mechanism for introducing a gas to form a movable aerodynamic seal between the elements. In this manner a chamber having a controllable interior environment is formed. One of the elements may have at least a portion of a device for dispensing reagents sealingly affixed therein. The other element may be adapted for introduction of a support into the interior of the chamber formed by the top and the bottom element. The apparatus may be used in methods for synthesizing biopolymers on a support.

Method and device for investigating substance libraries

The aim of the invention is to investigate the bonding of substances to target molecules. This is achieved by means of a densely packed device wherein various target molecules are bonded in a large number of sample areas. Cross-contamination and evaporation need to be minimized. The active surface of the sample areas have to be maximized. The inventive solution resides in the use of carrier plates, containing densely packed capillary structures and having a very large inner surface despite small outer dimensions. 1000 times more molecules can be bonded than on a flat outer surface of a comparable size. Cross contamination is avoided by the lack of cross links between the capillaries. Evaporation is minimized by a small outer surface. After the inner surface of the capillaries has been silanized, peptides and peptidomimetics are synthesized in a locally targeted manner. The molecular interactions of components of the substance library with active substances in a solution or a suspension are investigated by means of a local resolution optical detection method. Handling, especially cleaning and covering with substances, is carried out in a simple manner by rinsing liquids through the capillary plate.

A solid phase synthesis reaction vessel and method of using the same

A solid phase synthesis reaction vessel (1) includes a vessel (1) with a first inlet (3), a second inlet (5), and an outlet (7). A first stopcock (11) is disposed within the first inlet (3) and a second stopcock (13) is disposed within the outlet (7). Solid phase material is prevented from escaping the reaction vessel (1) via the outlet (7) while allowing fluid to flow through the outlet by a frit (9). In one embodiment, the reaction vessel (1) includes an outer hollow shell (31, 34) surrounding the vessel (1) to allow cooling or heating fluid to flow therein for controlling the temperature in the interior reaction volume (15). The solid phase synthesis reaction vessel (1) provides for the independent opening and closing of each of the inlets (3) and outlet (7) to provide for various ways of draining the vessel (1), varying the conditions within the vessel (1), and/or adding additional materials into the vessel (1).

Method and device to remove fluid and vapor

Microarray processing apparatus, well plate for microarray processing apparatus, microarray holder, and microarray cleaning method

Method for removing fluid and vapor

Combinatorial chemical synthesis

Apparatus for performing chemical reactions that occur simultaneously or continuously

Device for surface functionalization and detection

A fluidic device (100) is described for locally coating an inner surface of a fluidic channel. The fluidic device (100) comprises a first (101), a second (102) and a third (103) fluidic channel intersecting at a common junction (105). The first fluidic channel is connectable to a coating fluid reservoir and the third fluidic channel is connectable to a sample fluid reservoir. The fluidic device (100) further comprises a fluid control means (111) configured for creating a fluidic flow path for a coating fluid at the common junction (105) such that, when coating, a coating fluid propagates from the first (101) to the second (102) fluidic channel via the common junction (105) without propagating into the third (103) fluidic channel. A corresponding method for coating and for sensing also has been disclosed.

Laboratory reactor apparatus for automated synthesis of peptides

Automated polymer-synthesis system

Embodiments of the present invention are directed to automated-polymer-synthesis systems that include discrete reagent-solution-addition, wait-time, and reagent-solution-draining sub-systems which together significantly increase throughput and decrease sub-system idle time. The automated-polymer-synthesis systems that represent embodiments of the present invention additionally include switches at points in which carriers can be received from multiple input paths or output to multiple different output paths. The automated-polymer-synthesis systems that represent embodiments of the present invention generally include an input spur and output spur in addition to a main loop, allowing carriers containing only completed polymers to be removed and new carriers input, so that carriers traverse the automated-polymer-synthesis systems independently from one another.

Device for metering powder for chemical production processes under clean room conditions, use of same, and metering method

The invention relates to a device for metering powder (59), in particular into clean rooms, having a container (56) with powder (59) and a closure head (57) with a septum (115) for the container (56). The closure head (57) can be connected to the container (56) in a powder-tight manner, and the septum (115) can be connected to the closure head (57) in a powder-tight manner. The device additionally has a container receiving area (58), which is used to receive the closure head (57) of the container (56), and the opening (56a) of the container (56) points downwards such that the powder (59) can be poured out of the container (56). A gap (200) is provided between the closure head (57) and the receiving bowl (131) of the container receiving area (58), wherein a gas flow between the receiving bowl (131) and the closure head (57) can be produced in said gap. The invention also relates to the use of the device and to a metering method.

DISPENSING DEVICE FOR MULTIPLE CHANNEL REAGENTS AND DISPENSING PROCEDURE.

Cabezal dispensador de reactivos de múltiples canales (14a, 14b) para introducir una pluralidad de reactivos en una celda (24), comprendiendo el cabezal dispensador: un cuerpo del cabezal dispensador que tiene un extremo dispensador con un tamaño de salida comparable con el tamaño de la celda (24); y un grupo de boquillas (34) que incluyen una pluralidad de boquillas dispensadoras de reactivo que se disponen de modo que estén fluidamente acopladas con una pluralidad defuentes de reactivo (12a, 12b), estando el grupo de boquillas agrupado para proporcionar una pluralidad de salidas de boquillas en el cuerpo del cabezal dispensador en comunicación fluida con el extremo dispensador para permitir que una pluralidad de reactivos de la pluralidad de fuentes de reactivo se dispense al mismo tiempo a la celda (24) a través del extremo dispensador del cuerpo del cabezal dispensador. Multi-channel reagent dispensing head (14a, 14b) for introducing a plurality of reagents into a cell (24), the dispensing head comprising: a body of the dispensing head having a dispensing end with an output size comparable to the size of the cell (24); and a group of nozzles (34) that include a plurality of reagent dispensing nozzles that are arranged so that they are fluidly coupled with a plurality of reagent sources (12a, 12b), the nozzle group being grouped to provide a plurality of outlets of nozzles in the body of the dispensing head in fluid communication with the dispensing end to allow a plurality of reagents of the plurality of reagent sources to be dispensed at the same time to the cell (24) through the dispensing end of the body of the dispensing head .

Device for powder metering for chemical manufacturing processes under clean room conditions, its use and method of metering addition

Device for capturing beads and method and apparatus for arraying beads

Beads having diameters of several tens of micrometers to several millimeters and immobilized with biomolecules are captured by one kind of bead capturing nozzle one by one without fail. Using a bead holding plate having a plurality of wells each of which holds a plurality of the beads and a solution, a vibration generator mounted with a stage to attach the bead holding plate, and a bead capturing nozzle for bead capture connected to a suction pump, only a single bead is captured at the tip of the bead capturing nozzle by inserting the nozzle having an inner diameter smaller than the beads and a negative pressure inside created by the pump into the solution to allow the nozzle to come in contact with the beads in the well, applying a vibration to the bead holding plate by the vibration generator, and withdrawing the bead capturing nozzle into the air.

Device for surface functionalization and detection

A fluidic device (100) is described for locally coating an inner surface of a fluidic channel. The fluidic device (100) comprises a first (101), a second (102) and a third (103) fluidic channel intersecting at a common junction (105). The first fluidic channel is connectable to a coating fluid reservoir and the third fluidic channel is connectable to a sample fluid reservoir. The fluidic device (100) further comprises a fluid control means (111) configured for creating a fluidic flow path for a coating fluid at the common junction (105) such that, when coating, a coating fluid propagates from the first (101) to the second (102) fluidic channel via the common junction (105) without propagating into the third (103) fluidic channel. A corresponding method for coating and for sensing also has been disclosed.

Device for parallel and synchronous injection for sequential injection of different reagents

The invention relates to a microfluidic device for injecting series of mobile reaction chambers ( 102, 103 ) having non-miscible segmenters ( 101 ) in micro-channels ( 21 to 26 ), comprising: injection means ( 10 ) for injecting into microreaction channels alternatingly and in parallel liquid to form mobile reaction chambers and liquid for forming the segmenters; means for controlling the progression of one of the two liquids, applied to act on one zone ( 31 ) of each microchannel delimiting an injection volume of said liquid; the control means being able to cause stopping or slowing of the progression of said liquid over the zone of each microchannel by exerting an action based on a physico-chemical property of the liquid and said action not affecting the other liquid.

Microfluidic selection of library elements

Disclosed herein is a system comprising a chip; a flow channel disposed in the chip; the flow channel being in communication with an entry port and an exit port; the flow channel being operative to permit the flow of a library from the entry port to the exit port; a substrate; the substrate being disposed upon the chip; the substrate being operative to act as an upper wall for the flow channel; and a receptor; the receptor being disposed on the substrate; the receptor being operative to interact with a component from the library.

Microreactor array platform

A microreactor array platform and method for sealing a reagent in microreactors of an array of microreactors are provided. The microreactor array platform includes an array of microreactors, and a sealing film having a first surface and an opposite second surface, the sealing film configured to movably seal the array of microreactors. The microreactor array platform also includes an injector for delivering a reagent into the array of microreactors via a fluid path between the array and the second surface of the sealing film, and an applicator for directing a sealing liquid against the first surface of the sealing film. The microreactor array platform further includes a system for creating a pressure differential between the reagent in the injector and a space between the array of microreactors and the second surface of the sealing film.

A microarray assembly comprising a microporous membrane and an incubation chamber arrangement

The present invention relates to a microarray assembly adopted for carrying out a chemical reaction, in particular a hybridization reaction, comprising a microporous membrane having a plurality of different analyte-specific capture molecules attached in a microarray format, said membrane being arranged in an incubation chamber, said device further comprising: a receptacle for receiving said microporous membrane, sealing means for substantially encapsulating said membrane in opposition to said receptacle, so that said membrane is urged between said receptacle and said sealing means, at least one inlet port for supplying a fluid to a first cavity adjacent to a first end of said membrane, and a second cavity arranged at a substantially opposite second end of said membrane, thereby allowing a fluid flow from said first to said second cavity through said membrane.

Suction device enabling fine solid particles to be separated from a liquid

Device for separating the liquid and solid constituents of a chemical reaction mixture (1), comprising a metal filtering needle (12) provided with lateral holes (5) and able to pass through polymeric- or rubber-type membranes (3) without the latter losing their gastightness (2), a suction source (7) capable of sucking out a liquid from a closed vessel (8), a liquid evacuation channel (6) and an end-piece (4).

Reaction plenum with magnetic separation and/or ultrasonic agitation

The inventive apparatus is comprised of a means for producing cavitation in a complex reaction mixture to enhance the yield of the selected reaction product, a means for controlling the temperature of the complex reaction mixture, especially during cavitation, and a means for affecting magnetic separation of paramagnetic beads to which the selected reaction product is attached from the complex reaction mixture. In one embodiment, the compound(s) or molecule(s) is synthesized in situ, and isolated using the inventive apparatus. The apparatus finds use in the fields of solid phase organic synthesis, and for isolation and purification of a selected compound(s) or molecule (s), especially where automation is desired.

Contact unit comprising at least one reaction container integrated therein or attached thereto

Twelve reaction vessels ( 2 ) in two parallel rows are attached to a switch block ( 401 ). The switch block ( 401 ) comprises a reaction block ( 403 ) and twelve slide plates ( 404 ), which are arranged within it and which can be adjusted between an open position, a closed position and a further position. In the open position, access in straight line to the associated reaction vessel ( 2 ) is possible whereas in the closed position, the corresponding reaction vessel ( 2 ) is closed in a pressure-tight manner by the switch block ( 401 ). In the case of a positive pressure or depression in the reaction vessel ( 2 ), the corresponding slide plate ( 404 ) is pressed by this positive pressure or depression against the reaction block ( 403 ) or is drawn onto it. By means of this switch block ( 401 ), it is also possible to carry out processes in which high positive pressures are present in the reaction vessels ( 2 ).

Reaction block assembly for chemical synthesis

The reaction block assembly of the invention comprises a reaction block in which are disposed a plurality of reaction wells. Influent and effluent ducts open at the upper face of the reaction block adjacent each of the reaction wells. These ducts provide communication between each reaction well and a source of reagents and gases and with waste removal means for removal of effluent from the reaction wells. A membrane sheet overlies the upper face of the reaction block to define a pneumatic valve to open and close the duct openings each of the reaction wells to control the input of reagents and gases and effluent flow from the reaction wells. The reaction block assembly preferably includes a temperature control block that underlies the reaction block for control of the temperature in the reaction wells. A waste block underlies the reaction block or, if used, the temperature control block. The effluent ducts from the reaction wells communicate with the waste block for removal of reaction well effluent from the reaction block assembly. Overlying the reaction block is a top manifold plate block for delivery of high pressure gas to actuate the pneumatic valves by applying pressure against the membrane to seal the duct openings.

Method and device to remove fluid and vapor

The present invention relates to a method and device for the removal of fluids and vapors in containment, from adjacent surfaces including supporting flat surfaces and substrates, such as protein spots printed in arrays and micro-array formats. This method and device removes fluids and vapors under controlled and repeatable conditions, enabling uniform phases for degree of drying incurred by objects, specimen and structures, including tissues, adsorbed particles and biological substrates, while reducing and preventing impact of meniscus phase surface tension forces.

Combinatorial chemical synthesis

Combinatorial synthesis methods obtain a plurality of compositions having materially different characteristics using an apparatus having a plurality of collectors. A first quantity of fluid reactants are reacted to form a first quantity of product composition. Following completion of the collection of the first quantity of product composition, a second quantity of fluid reactants are reacted to form a second quantity of product composition, the second quantity of product composition being material different from the first quantity of product composition. An apparatus includes a nozzle connected to a reactant source and a plurality of collectors. The nozzle and plurality of collectors move relative to each other such that a collector can be selectively placed to receive a fluid stream emanating from the nozzle. The plurality of product compositions can be evaluated to determine their suitability for various applications.

Apparatus for carrying out biochemical reactions

An apparatus for carrying out biochemical reactions in microtiter plates comprises means (42, 43) for receiving and holding a microtiter plate (44) to be processed, the microtiter plate being provided with a plurality of wells and each well having a bottom outlet, and a movably mounted device (47) for the application of gas pressure to the wells of the microtiter plate (44), the pressure device comprising sealing means (48) and being movable between a position where it leaves the upper part of the microtiter plate accessible, and a position where it sealingly contacts the microtiter plate through the sealing means (48) so that a gas pressure may be applied to the inlets of said microtiter plate wells for pressing out liquid present therein through the bottom outlet of each well.

Multi-channel peptide synthesizer and operating method thereof

Disclosed is a multi-channel peptide synthesizer, including a gas-bath thermotank, a plurality of reactor tubes, a motor, a rotating rack, a liquid-feeding tube, a feeding device, a vacuum tube and a nitrogen tube. The gas-bath thermotank body provides a desired constant temperature for reaction. The reactor tube provides a place for peptide synthesis and resin washing. The motor and the rotating rack are used to fully mix the reaction and cleaning solutions. Various liquid reagents required are fed to the reactor tube through the liquid-adding tube. Various materials required are prepared in advance in the feeding device and directly fed to the reactor tube. The reaction or washing solution in the reactor tube is pumped to a waste liquid tank through the vacuum tube. Nitrogen is introduced into each reactor tube through the nitrogen tube. This device can be applied in batch-wise peptide synthesis using solid-phase methods.

Device for powder metering for chemical production processes under clean-room conditions, use thereof and metered addition method

本發明係關於一種用於對粉末(59)進行計量，尤其在潔淨室中對粉末(59)進行計量之裝置，其包含容納粉末(59)之容器(56)及用於該容器(56)之具有隔板(115)的密封頭(57)，其中該密封頭(57)可與該容器(56)不透粉末地連接且該隔板(115)可與該密封頭(57)不透粉末地連接，且該裝置進一步包含用以固持該容器(56)之該密封頭(57)的容器固持器(58)，且該容器(56)之開口(56a)指向下方，以使得該粉末(59)可自該容器(56)流出，其中在該密封頭(57)與該容器固持器(58)之固持槽(131)之間提供間隙(200)，在該間隙中可產生該固持槽(131)與該密封頭(57)之間的氣流(關於此參見圖5a)。 本發明亦關於該裝置之用途及一種計量添加方法。

Compact dispenser

A flow through dispenser for the drop-wise dispensation of liquid which arrangement comprises: a housing ( 104 ) comprising: (i) a flow through microconduit ( 105 ) with an upstream end ( 106 ) and a downstream end (outlet) ( 107 ); and (ii) a dispenser orifice ( 108 ) between these two ends, and an inlet tube ( 110 ) which is attached to the upstream end ( 106 ) and provides an inlet ( 111 ) that can be connected to a liquid storage ( 112 ) for liquid ( 113 ) that is to be transported in flow through microconduit ( 105 ) and inlet tube ( 110 ) and/or dispensed through the orifice ( 108 ). The inner volume between the inlet and the downstream end is is ≦10 μl. An instrument set-up for drop-wise dispensation of liquid to target areas of a microdevice. The characteristic feature comprises: a) a flow through drop dispenser ( 202 ) comprising: one or more flow through paths ( 220 ) which each has an outlet ( 221 ), an inlet ( 222 ), and a dispenser orifice ( 208 ); and b) a generator for liquid transport ( 217 ) by aspirating or pushing liquid through said paths ( 220 ) from the inlet(s) ( 211 ) to the outlet(s) ( 221 ), pushing being accomplished by using over pressure of gas upstream the inlet end ( 211 ).

Suction device enabling fine solid particles to be dissociated from a liquid

Dispositif de séparation des constituants liquides et solides d'un milieu réactionnel chimique (1) comprenant une aiguille filtrante (12) métallique pourvue de trous latéraux (5) permettant de passer à travers des membranes de type polymérique ou caoutchouteux (3) sans que celles-ci perdent leur étanchéité aux gaz (2), une source aspirante (7) capable d'aspirer un liquide hors d'une enceinte close (8), un canal d'évacuation du liquide (6), et un embout (4).

System and method for high throughput processing of droplets

A method for high throughput processing of a plurality of droplets. The droplets are dispensed onto a moving surface and delayed in a delay line in which the droplets hang from the moving surface for at least a specified minimum period of time. A laminate may be spooled onto the moving surface and each droplet may be dispensed onto the laminate. At least one operation is performed on each droplet from the group of operations consisting of mixing, diluting, concentration, heating, cooling, humidifying, filtering, and analyzing. The laminate may then, in certain embodiments, be spooled off the moving surface, processed, and reused.

Device for parallel oligomer synthesis, method of parallel oligomer synthesis and use thereof

Small distribution device

Systems, apparatus and kits for enzymatic polynucleotide synthesis

The invention is directed to systems, apparatus and kits for automated synthesis of a plurality of polynucleotides in an array of reaction chambers using a template-free polymerase. In some embodiments, adaptive elements and processes are provided to monitor and control disruption of the synthesis process and fluid movement by enzyme aggregation.

Nucleic acid integrated detection method and detection reagent tube

Microfabricated flowthrough porous apparatus for discrete detection binding reactions

An improved apparatus for conducting a multiplicity of individual and simultaneous binding reactions is described. The apparatus comprises a substrate on which are located discrete and isolated sites for binding reactions. The apparatus is characterized by discrete and isolated regions that extend through a substrate and terminate on a second surface thereof, such that when a test sample is applied to the substrate, it is capable of penetrating through each such region during the course of the binding reaction. The apparatus is especially useful for sequencing by hybridization of DNA molecules.

Device and method for the transfer of liquid samples

The present invention relates to a transfer device ( 1 ) for removing fluid samples ( 2 ) from containers ( 3 ) and for introducing these fluid samples ( 2 ) into chambers ( 5 ) positioned below these containers, the device ( 1 ) including an individual chamber ( 5′, 5 ″) for each of the containers ( 3, 3 ′). Transfer devices ( 1 ) according to the present invention are distinguished in that they have enclosure means ( 4 ), which include one single individually assigned restriction opening ( 10 ), which limits the flow of fluids to be introduced into the containers ( 3, 3 ′) or removed from the chambers ( 5, 5′, 5 ″), for each container ( 3, 3 ′) or for each chamber ( 5, 5′, 5 ″). Furthermore, the present invention relates to a method of removing fluid samples ( 2 ) from containers ( 3 ) and of introducing these fluid samples ( 2 ) into chambers ( 5 ) positioned below these containers using such a transfer device ( 1 ). The device ( 1 ) may be used for individually immobilizing fluid samples ( 2 ) on MALDI-MS targets ( 21 ) and/or for collecting liquid samples in individual collection spaces ( 12 ).

Device for surface functionalization and detection

A fluidic device (100) is described for locally coating an inner surface of a fluidic channel. The fluidic device (100) comprises a first (101), a second (102) and a third (103) fluidic channel intersecting at a common junction (105). The first fluidic channel is connectable to a coating fluid reservoir and the third fluidic channel is connectable to a sample fluid reservoir. The fluidic device (100) further comprises a fluid control means (111) configured for creating a fluidic flow path for a coating fluid at the common junction (105) such that, when coating, a coating fluid propagates from the first (101) to the second (102) fluidic channel via the common junction (105) without propagating into the third (103) fluidic channel. A corresponding method for coating and for sensing also has been disclosed.

Method for isolation of independent, parallel chemical micro-reactions using a porous filter

The present invention relates to the field of fluid dynamics. More specifically, this invention relates to methods and apparatus for conducting densely packed, independent chemical reactions in parallel in a substantially two-dimensional array. Accordingly, this invention also focuses on the use of this array for applications such as DNA sequencing, most preferably pyrosequencing, and DNA amplification.