Auf einer Oberfläche befestigte multifunktionelle Polymere-Netzwerke für Sensorchips

HYBRID PROCEDURE FOR THE PRODUCTION OF CARRIERS FOR THE ANALYTBESTIMMUNG

MICRO ARRAY DEVICES WITH CONTROLLABLE REACTION VOLUME

DEVICES AND PROCEDURES FOR MEASURING THE STORAGE REACTION

DEVICE IN FORM OF A MICRO TITER PLATE TO THE MULTIPLEXIERTEN ARRAY

PROCEDURE FOR THE PRODUCTION OF STRUCTURED ONE, SELFORGANIZED MOLECULAR ONE MONO SITUATIONS OF INDIVIDUAL MOLECULAR SPECIES, IN PARTICULAR OF SUBSTANCE LIBRARIES

PROCEDURE FOR THE PRODUCTION OF MANUFACTURING CHEMICAL SURFACES

PROCEDURE FOR GENERATING INFORMATION IN CONNECTION WITH THE MOLECULAR STRUCTURE OF A BIO MOLECULE

DEVICE FOR THE EXECUTION OF CHEMICAL AND BIOCHEMICAL REACTIONS WITH USE OF PHOTO-PRODUCED REAGENTS

SYNTHESIS OF COMBINATORIAL LIBRARIES

Cellular microarrays for screening differentiation factors

Provided is a microarray platform for the culture of cells atop combinatorial matrix mixtures; enabling the study of differentiation in response to a multitude of microenvironments in parallel.

Coded nucleic acid carriers

Laminated assembly for active bioelectronic devices

1-thiogalactose derivatives

1-galactose derivatives

METHOD FOR ANALYZING BIOMOLECULE

In a method for analyzing a biomolecule by localizing the biomolecule on a solid phase and detecting the biomolecule as a visible two-dimensional signal, the signal is detected by capturing a two-dimensional image on the solid phase with use of a scanner comprising a light source for irradiating a light on the solid phase and an image sensor for receiving a reflected light from the solid phase and capturing the two-dimensional image on the solid phase by two-dimensionally scanning the solid phase, and analyzing obtained two-dimensional image data.

COMBINATORIAL COMPLEX CARBOHYDRATE LIBRARIES AND METHODS FOR THE MANUFACTURE AND USES THEREOF

A combinatorial complex carbohydrate library is provided and including a plurality of addressable complex carbohydrate structures.

APPARATUS AND METHODS FOR THE AUTOMATED SYNTHESIS OF OLIGOSACCHARIDES

One aspect of the present invention relates to an apparatus for the efficient synthesis of oligosaccharides on a solid support, e.g., formed by subunit addition to terminal subunits immobilized on solid-phase particles. In certain embodiments, the apparatus of the present invention is used in combinatorial methods, e.g., as described herein, of synthesizing oligosaccharides.

BUILDING BLOCKS FOR ARTIFICIAL RECEPTORS

The present invention relates to building blocks for making or forming candidate artificial receptors. A building block can provide one or more structural characteristics such as positive charge, negative charge, acid, base, electron acceptor, electron donor, hydrogen bond donor, hydrogen bond acceptor, free electron pair, Ir electrons, charge polarization, hydrophilicity, hydrophobicity, and the like. A building block can be bulky or it can be small.

TARGET PLATE FOR MASS SPECTOMETERS AND USE THEREOF

A plate suitable for use with mass spectrometers comprising a number of target spots arranged in a surface portion of said plate making it possible to deposit small amounts of fluid at said target spots without the fluid escaping or getting mixed with fluid deposited at another target surface of the same plate. The target surfaces being arranged in a surface portion of said plate so that a base material of said plate constitutes the walls of receptacles, characterised in that the shape, size, temperature and possible agents of said receptacle facilitate evaporation of a solution in which sample molecules are suspended. Embodiments include different types of matrix and enzymes arranged at said spots, and methods for enhancing MALDI analysis efficiency.

MICRODEVICES HAVING A PREFERENTIAL AXIS OF MAGNETIZATION AND USES THEREOF

The invention relates generally to the field of moiety or molecule isolation, detection and manipulation and library synthesis. In particular, the invention provides a microdevice, which microdevice comprises: a) magnetizable substance; and b) a photorecognizable coding pattern, wherein said microdevice has a preferential axis of magnetization. Systems and methods for isolating detecting and manipulating moieties and synthesizing libraries using the microdevices are also provided.

SYNTHESIS OF OLIGOSACCHARIDES, REAGENTS AND METHODS RELATED THERETO

One aspect of the present invention relates to differentially protected glycosyl phosphates. Another aspect of the present invention relates to the preparation of glycosyl phosphates from glycal precursors. In another aspect of the present invention, glycosyl phosphates are used as glycosyl donors in glycosylation reactions.

DEVICE FOR CONTAINING, REACTING AND MEASURING, AND METHOD OF CONTAINING, REACTING AND MEASURING

The invention relates to a device for containing, reacting and measuring, and a method of containing, reacting and measuring, and provides a device for containing, reacting and measuring, and a method of containing, reacting and measuring which is also able to effectively and quickly perform the reaction processing, measuring and identification. The invention comprises; a transparent container section having a liquid inlet/outlet and which is able to contain a base member with several kinds of substances for detection having predetermined chemical structures fixed at respective fixed positions which are arranged at predetermined spacing, and with each of the chemical structures associated with each of the fixed positions, a drawing and discharging section which is able to draw and discharge the liquid into and from the container section via the inlet/outlet, and a measuring device which is able to receive light from the contained base member, external to the container section and in a condition ...

MICROFLUIDIC MICROARRAY ASSEMBLIES AND METHODS OF MANUFACTURING AND USING SAME

The invention encompasses microfluidic microarray assemblies (MMA) and subassemblies and methods for their manufacture and use. In one embodiment, first and second channel plates are provided and are sealingly connected to a test chip in consecutive steps. Each plate includes microfluidic channels configured in a predetermined reagent distribution pattern. The test chip comprises a plurality of discrete test positions, each test position being located at the intersection between a first predetermined reagent pattern and a second predetermined reagent pattern, wherein at least one of said patterns is non-linear. The first channel plate allows the distribution of a first reagent on said test chip, wherein said first reagent is immobilized at said test positions. The second channel plate allows the distribution of a second reagent on said test chip, wherein said second reagent comprises a plurality of different test samples.

PATTERNING WITH COMPOSITIONS COMPRISING LIPID

Better patterning methods, including for : better methods for forming biomolecular arrays, including a method comprising: -providing a tip and a substrate surface, disposing a patterning composition at the end of the tip, depositing at least some of the patterning composition from the tip to the substrate surface to form a deposit disposed on the substrate surface, wherein the patterning composition comprises at least one lipid, optionally at least one solvent, and at least one patterning species different from the lipid and the optional solvent. The lipid can be a phospholipid such as DOPC (1, 2-di-oleoyl-sn-glycero-3-phosphocholine). The, patterning species can be an oligonucleotide or a protein. Microarrays and nanoarrays can be prepared including nanoscale resolution of deposits. The lipid can activate patterning or increase the rate of patterning. Simplified tip preparation can be achieved. Nanoscopic, SPM (atomic force microscope), and AFM (scanning probe microscope) tips can be used ...

SELF-ADDRESSABLE SELF-ASSEMBLING MICROELECTRONIC SYSTEMS ANDDEVICES FOR MOLECULAR BIOLOGICAL ANALYSIS AND DIAGNOSTICS

A self-addressable, self-assembling microelectronic device is designed and f abricated to actively carry out and control multi-step and multiplex molecular biological reactions in microscopic formats. These reactions include nucleic acid hybridization, antibody/a ntigen reaction, diagnostics, and biopolymer synthesis. The device can be fabricat ed using both microlithographic and micro-machining tech niques. The device can electronically control the transport and attachment of specif ic binding entities to specific micro-locations. The sp ecific binding entities include molecular biological molecules such as nucleic acids and po lypeptides. The device can subsequently control the tra nsport and reaction of analytes or reactants at the addressed specific micro-locations. The device is able to concentrate analytes and reactan ts, remove non-specifically bound molecules, provide stringency control for DNA hybridi zation reactions, and improve the detection of analytes. The device can be electronically ...

SYNTHESIS OF COMBINATORIAL ARRAYS OF ORGANIC COMPOUNDS THROUGH THE USE OF MULTIPLE COMPONENT COMBINATORIAL ARRAY SYNTHESES

The present invention relates to an array of compounds having a common core structure wherein the compounds of the array comprise the products of a multiple component combinatorial array synthesis having at least three components. The present invention also relates to the method of synthesizing that array. A further embodiment of the present invention relates to the use of solid phase synthesis to synthesize the combinatorial array of compounds. The present invention also relates to a method of creating a combinatorial array of compounds with a common core structure by identifying the desired core structure, identifying an MCCA reaction capable of generating that core structure, followed by preparing an array of compounds using the identified MCCA reaction according to the aforementioned method. The present invention also relates to a method for conducting in vitro assays of biological material using the combinatorial arrays of the present invention.

SYNTHESIS OF COMBINATORIAL LIBRARIES

Directly dividing the contents of each sub-pool into the subpools for the next step in the synthetic scheme for producing a combinatorial library reduces the standard deviation, .sigma., relative to the standard deviation of the split synthesis method for producing such libraries.

METHODS FOR SCREENING COMPOUND LIBRARIES

Disclosed are methods for screening compound libraries using frontal chromatography in combination with mass spectrometry to identify and rank those members of the library that bind to a target receptor. Methods are also disclose d which permit a compound library to be rapidly screened to determine if any members of the library have a higher affinity for the target receptor relative to a pre-selecte d indicator compound.

AFFINITY BASED SELF-ASSEMBLY SYSTEMS AND DEVICES FOR PHOTONIC AND ELECTRONIC APPLICATIONS

This invention relates to techniques which utilize programmable functionalized self-assembling nucleic acids, nucleic acid modified structures, and other selective affinity or binding moieties as building blocks. The invention is a method for the fabrication of micro scale and nanoscale devices comprising the steps of: fabricating first component devices on a first support, releasing at least one first component device from the first support, transporting the first component device to a second support, and attaching the first component device to the second support. The invention also provides for orienting a structure in an electric field, reacting affinity sequences, and assembling chromophoric structures by photoactivation.

SUBSTRATE FOR MICROARRAY AND MICROARRAY HAVING PATTERNED THIN LAYER AND METHOD FOR PRODUCING SAME WHICH ALLOWS TO INCREASE SIGNAL-TO-NOISE OF SPOT REGION WITH REGARD TO BACKGROUND SIGNAL

PURPOSE: A substrate for microarray and a microarray having a patterned thin layer and a method for producing the same substrate and microarray are provided to increase the signal-to-noise of the spot region with regard to the background signal. CONSTITUTION: The substrate for the microarray having a patterned thin layer is provided, wherein the substrate and the thin layer have different refractive index each other; the patterned thin layer comprises a spot region having the thickness such that a first reflected light from the surface and a second reflected light from the thin layer result in constructive interference, and blank region having the thickness such that a first reflected light from the surface and a second reflected light from the thin layer result in destructive interference; the substrate is made of one selected from glass, silicon wafer, fused silica, gold, silver, copper, platinum, polystyrene, poly(methylacrylate) and polycarbonate; and the thin layer is selected from ...

LAMINATED ASSEMBLY FOR ACTIVE BIOELECTRONIC DEVICES

PURPOSE: Devices containing active electrodes especially adapted for electrophoretic transport of nucleic acids, their hybridization and analysis, are provided. CONSTITUTION: Methods of manufacture and devices for performing active biological operations utilize laminated structures(30). A first planar sample support(50) includes at least one sample through hole(56) a planar electrode(32,70) is disposed adjacent the first planar sample support(50), and includes an electrode through region(38), a second planar support(40) includes a vent through hole(48), the planar electrode(32) being in a laminated relationship between the first planar sample support(50) and the second planar support(40), further characterized in that the sample through hole(56), electrode through hole(38) and vent through hole(48) are in overlapping arrangement. Preferably, some or all of the through holes, through regions and vent through holes are aligned. COPYRIGHT 2000 KIPO ...

FLOW CELL DEVICES, SYSTEMS AND METHODS OF USING THE SAME

Flow cell devices and methods for using the devices are disclosed. In one aspect, the flow cell devices are used to expose substrate surfaces comprising biopolymers or monomers to a desired fluid. The flow cell assemblies comprise a flow cell chamber (2) for receiving a substrate and a bottom manifold (3) in fluid communication with the chamber (2) for feeding a fluid via a plurality of inlet ports (4) . The bottom manifold comprises an entry conduit (5) which communicates with a first end of the flow cell chamber via an entry port (6) and an exit conduit (7) which communicates with a second end of the flow cell via exit port (8) . The side of the bottom manifold comprises openings that connect or are co-extensive with the inlet ports (4) . The flow cell may also comprise a similar top manifold (9). Workstations are also provided including a flow cell device and one or more station (s) for processing a substrate, such as one or more of a printer, reaction chamber, wash chamber, and scanner ...

NOVEL STRATEGIE FOR SYNTHESISING POLYMERS ON SURFACES

The invention relates to a novel method for synthesising polymers, particularly biopolymers such as nuclear acids, peptides and saccharides, on the surface of solid supports. The inventive method enables improved quality polymers to be produced at a lower cost. Novel structures of support-bound polymers are also disclosed.

NANOPARTICLES HAVING OLIGONUCLEOTIDES ATTACHED THERETO AND USES THEREFOR

The invention provides methods of detecting a nucleic acid. The methods comprise contacting the nucleic acid with one or more types of particles having oligonucleotides attached thereto. In one embodiment of the method, the oligonucleotides are attached to nanoparticles and have sequences complementary to portions of the sequence of the nucleic acid. A detectable change (preferably a color change) is brought about as a result of the hybridization of the oligonucleotides on the nanoparticles to the nucleic acid. The invention also provides compositions and kits comprising particles. The invention further provides methods of synthesizing unique nanoparticle-oligonucleotide conjugates, the conjugates produced by the methods, and methods of using the conjugates. In addition, the invention provides nanomaterials and nanostructures comprising nanoparticles and methods of nanofabrication utilizing nanoparticles. Finally, the invention provides a method of separating a selected nucleic acid from ...

ELECTRODE ARRAY DEVICE HAVING AN ADSORBED POROUS REACTION LAYER HAVING A LINKER MOIETY

There is disclosed an electrode array device having an adsorbed porous reaction layer having a linker attacher thereto for improved synthesis quality. The array comprises a plurality of electrodes on a substrate, wherein the electrodes are electronically connected to a computer control system. The array has an adsorbed porous reaction layer having a linker attached thereto on the plurality of electrodes, wherein the adsorbed porous reaction layer comprises a chemical species having at least one hydroxyl group. In the preferred embodiment, the reaction layer is sucrose having an ionic linker comprised of DNA. In another preferred embodiment, the reaction layer is sucrose, fructose, and glucose having an ionic linker comprised of DNA.

METHOD FOR PREPARING SUBSTRATES HAVING IMMOBILIZED MOLECULES AND SUBSTRATES

A method for the efficient immobilization of molecules onto substrate surfaces that employs an isocyanate compound to form a reactive isocyanate surface, nanoparticles onto surfaces as well as silylated molecules such as silylated oligonucleotides or proteins onto unmodified surfaces such as a glass surface is provided. Also provided are compounds, devices, and kits for modifying surfaces such as glass surfaces.

Methods and compositions for producing biopolymeric arrays

Methods and compositions are provided for producing arrays of polymeric binding agents. In the subject methods, the individual polymers of the array are synthesized using solid phase synthesis techniques on the surface of a substrate. A critical feature of the invention is that one or more locations on the substrate surface are spatially and temporally protected by a protective bubble during the synthesis protocol, where the protective bubble may be produced using any convenient bubble producing means. The bubble producing means may be a component of either a substrate or a structure separate from the substrate. Also provided are the arrays produced by the subject methods, kits for use in practicing the subject methods, and methods of using the arrays in analyte detection assays, including hybridization assays, such as gene discovery, differential gene expression and gene sequencing assays.

High throughput mechanical rapid serial property testing of materials libraries

A library of materials is screened for mechanical properties such as surface tension or interfacial tension. A library of materials is provided. A stimulus such as a stress or force is provided to each member of the library. A response (e.g., a resistance) of each of the materials due to the stimulus is measured and the response, the stimulus or both are recorded and related to provide data. Thereafter, the data is analyzed to reach conclusions regarding the material samples.

Synthesizing and screening molecular diversity

A general stochastic method for synthesizing compounds can be used to generate large collections of tagged compounds that can be screened to identify and isolate compounds with useful properties.

Active programmable electronic devices for molecular biological analysis and diagnostics

A self-addressable, self-assembling microelectronic device is designed and fabricated to actively carry out and control multi-step and multiplex molecular biological reactions in microscopic formats. These reactions include nucleic acid hybridization, antibody/antigen reaction, diagnostics, and biopolymer synthesis. The device can be fabricated using both microlithographic and micromachining techniques. The device can electronically control the transport and attachment of specific binding entities to specific micro-locations. The specific binding entities include molecular biological molecules such as nucleic acids and polypeptides. The device can subsequently control the transport and reaction of analytes or reactants at the addressed specific microlocations. The device is able to concentrate analytes and reactants, remove non-specifically bound molecules, provide stringency control for DNA hybridization reactions, and improve the detection of analytes. The device can be electronically replicated.

Functionated photoacid generator and functionated polymer system for biological microarray synthesis

In some embodiment of the invention, methods are provided for the synthesis of polymer arrays. In one embodiment, a reactive polymer and a photo acid generator is used for the photodirected polymer array synthesis.

MICROARRAY DEVICES HAVING CONTROLLABLE REACTION VOLUME

Method of detection of molecular recognition by electrochemiluminescence

The invention provides an apparatus and method for determining a signal produced by a micro array device. The apparatus provides an unstructured probe (5) and structured probe (4). The unstructured probe binds to a target (18) and provides a first signal (14) that can be compared to a second signal (15) produced by a structured probe. A more accurate level of intensity of the first signal can be determined by comparing to the second signal produced by the structured probe. A method for determining a more accurate level of signal intensity produced from the unstructured probes bound to the target is also disclosed.

Process for producing array plate for biomolecules having hydrophilic and hydrophobic regions

A method for manufacturing an array plate for biomolecules and a method for manufacturing a biochip using this array plate are provided. The array plate manufacturing method includes: (a) coating a surface of a substrate (1) with a hydrophobic material to form a hydrophobic layer (3); (b) etching the hydrophobic layer through an etch mask (4) placed thereon to form a hydrophilic binding site (5); (c) removing the remaining etch mask; and (d) processing the remaining region of the hydrophobic layer (3) to recover its original hydrophobic properties. The biochip manufacturing method includes processing the surface of the hydrophilic binding site (5) of an array plate manufactured using the method, and applying a solution containing biomolecules to the surface of the hydrophilic binding site.

MICROARRAY AND ITS MANUFACTURING METHOD

PROBLEM TO BE SOLVED: To provide a microarray containing biological reaction molecules, its utilizing method, and its manufacturing method. SOLUTION: It is possible to manufacture a large number of the same arrays by preparing the arrays by slicing a bundle of capillaries or rods each containing a specific reactant. For example, under condition that fluorescence or chemiluminescent signals are obtained, various biochemical and quantitative analyses are performed on each array on the basis of nucleic acid hybridization and small molecular binding to obtain the images of the signals. By electronically processing the images of the signals, clinically and experimentally useful data is obtained in the method. Therefore, it is possible to simultaneously screen all the compounds on specific chemical and biological actions through the use of the arrays. COPYRIGHT: (C)2003,JPO ...

VERFAHREN ZUR PARALLELEN SYNTHESE UND ÜBERTRAGUNG VON MOLEKÜLEN AUF EIN SUBSTRAT

Apparat zur Durchführung von sich wiederholenden chemischen Reaktionen.

Microarray carrier containing colored glass fluorescent standard, useful for preparing DNA or protein arrays for e.g. diagnosis, enables instrument calibration and comparability of measurements between laboratories

Microarray carrier (1) comprises a substrate (2), preferably essentially non-fluorescent, and at least one standard (A) for fluorescent measurements, made of colored glass. An independent claim is also included for a biochip that comprises (1) and, on (1), at least one microarray of probe molecules.

Verfahren und Vorrichtung zur Herstellung in heterogener Phase von Makromolekülen wie Peptiden, Polynuklotiden oder Polysocchariden.

SELFORGANIZED SYSTEMS ON THE BASIS OF AFFINITY AND ARRANGEMENTS FOR PHOTO NICHE OR ELECTRONIC APPLICATIONS

COMBINATORIAL STRATEGY OF GROUP OF PROTECTION FOR MULTI-FUNCTIONAL MOLEKULE

PROCEDURE FOR THE PRODUCTION OF A CHIP FOR THE MOLECULAR PROOF

PROCEDURE FOR ARRANGING A POLYMER MOLECULE

MICRO-FLUID POORLY REACTIVE WITH THREE FLOW LEVELS

COMBINATORIAL ONE, COMPLEXES COAL HYDRATE LIBRARIES AND PROCEDURE FOR THE PRODUCTION AND FOR THE USE OF IT

METHOD AND SYSTEM FOR THE DETECTION OF CARDIAC RISK FACTORS

Saccharide derivatives

High throughput screening array containing porous material

Spatially addressable combinatorial chemical arrays in cd-rom format

Scanning optical detection system

Method for producing finished chemical surfaces

Photocleavable protecting groups

Apparatus and methods for the automated synthesis of oligosaccharides

METHOD AND DEVICE TO OPTIMIZE ANALYTE AND ANTIBODY SUBSTRATE BINDING BY LEAST ENERGY ADSORPTION

... ²²²The present invention provides a method of making an assay device for ²conducting an assay to detect a concentration of an analyte in a sample fluid. ²The assay devices would typically have a substantially planar surface having a ²series of site specific immobilized calibration spot arrays containing pre-²determined quantities of the analyte printed thereon. In addition, a series of ²site specific immobilized test spot arrays, including capture antibody for ²binding the analyte protein is printed on the assay device. The method ²involves first modifying the planar surface to provide hydrophobic binding ²sites, hydrophilic linking and covalent bonding sites. Then the method ²requires printing the series of site specific immobilized test spot arrays and ²the series of site specific immobilized calibration spot arrays on the ²substantially planar surface. Applying the sample fluid to the assay device is ²the next step followed by testing a sensitivity of the assay and modulating ²ratios ...

CODED NUCLEIC ACID CARRIERS

The present invention relates generally to coded solid or semi-solid nucleic acid carriers for use in multiplexing solid phase nucleic acid-based reactions. The use of coded carriers facilitates multiplexing due to the ability to deconvolute multiple nucleic acid-based events and to correlate these to particular experiments. The present invention further provides a method for identifying a nucleic acid molecule having a defined characteristic within a population of two or more different nucleic acid molecules using coded nucleic acid carriers. Conversely, the nucleic acid can be used as the code for a particular peptide, or other chemical, bound specifically to a microsphere with a specific oligonucleotide sequence. Alternatively, the method of the present invention permits screening for molecules which interact with target nucleic acid, or other, molecules. The method and the coded carriers of the present invention enable high throughput screening of nucleic acid, or other, molecules.

DEVICES AND METHODS FOR CARRYING OUT CHEMICAL REACTIONS USING PHOTOGENERATED REAGENTS

Fluidic methods and devices for conducting parallel chemical reactions are disclosed. The methods are based on the use of in situ photogenerated reagents such as photogenerated acids, photogenerated bases, or any other suitable chemical compounds that produce active reagents upon light radiation. The present invention describes devices and methods for performing a large number of parallel chemical reactions without the use of a large number of valves, pumps, and other complicated fluidic components. The present invention provides microfluidic devices that contain a plurality of microscopic vessels for carrying out discrete chemical reactions. Other applications may include the preparation of microarrays of DNA and RNA oligonucleotides, peptides, oligosacchrides, phospholipids and other biopolymers on a substrate surface for assessing gene sequence information, screening for biological and chemical activities, identifying intermolecular complex formation, and determining structural features ...

METHOD OF SYNTHESIZING DIVERSE COLLECTIONS OF OLIGOMERS

A general stochastic method for synthesizing random oligomers can be used to synthesize compounds to screen for desired properties. The use of identification tags on the oligomers facilitates identification of oligomers with desired properties.

APPARATUS AND METHODS FOR ACTIVE PROGRAMMABLE MATRIX DEVICES

A system for performing molecular biological diagnosis, analysis and multistep and multiplex reactions utilizes a self-addressable, self-assembling microelectronic system for actively carrying out controlled reactions in microscopic formats. Preferably, a fluidic system flows a sample across an active area of the biochip, increasing diagnostic efficiency. Preferably, the fluidic system includes a flow cell having a window. Pulsed activation of the electrodes of the biochip are advantageously employed with the fluidic system, permitting more complete sampling of the materials within the biological sample. An improved detection system utilizes a preferably coaxially oriented excitation fiber, such as a fiber optic, disposed within a light guide, such as a liquid light guide. In this way, small geometry systems may be fluorescently imaged. A highly automated DNA diagnostic system results. Perturbation of the fluorescence signal during electronic denaturation is detailed and analyzed for analytical ...

ELECTROSPRAY DEVICE FOR MASS SPECTROMETER

An electrospray apparatus employing multiple electrospray needles mounted in a circular arrangement sequentially delivers multiple sample streams to a mass spectrometer for analysis. One electrospray device includes an electrospray chamber, a rotatable needle supporting plate, a plurality of electrospray needles mounted on the plate, and a charger for applying a charge to droplets delivered to the electrospray chamber by the needles. Another electrospray device includes an electrospray chamber, a plurality of electrospray needles arranged in a circular arrangement, a charger, and a rotatable member for delivering gas phase ions from one needle at a time to the mass spectrometer. The rotatable electrospray apparatus provides fast repetitive analysis of simultaneously operating chromatography columns or other sample streams with a single mass spectrometer.

PROCESS SOL-GEL OF FONCTIONNALISATION Of a SURFACE Of a SOLID SUBSTRATE.

The molecular biological diagnostic system automated

PREPARATION AND USE OF A REACTIVE SOLID SUPPORT SURFACE

A method of preparing a protein-resistant reactive solid support surface is disclosed. The method comprises the steps of: a) providing a solid support having a hydrogel coating with a plurality of binding elements, b) coupling a protein resistant compound to the hydrogel via a first fraction of the binding elements, and c) coupling at least one binding agent to the hydrogel via a second fraction of the binding elements, whereby the protein resistant compound and the at least one binding agent are co-immobilized to the hydrogel. Also the use of the reactive surface in analysis, such as immunogenicity assays, is disclosed.

METHOD FOR PREPARING SUBSTRATES HAVING IMMOBILIZED MOLECULES AND SUBSTRATES

A method for the efficient immobilization of molecules onto substrate surfaces that employs an isocyanate compound to form a reactive isocyanate surface, nanoparticles onto surfaces as well as silylated molecules such as silylated oligonucleotides or proteins onto unmodified surfaces such as a glass surface is provided. Also provided are compounds, devices, and kits for modifying surfaces such as glass surfaces.

SURFACE-ATTACHED POLYFUNCTIONAL POLYMER NETWORKS FOR SENSOR CHIPS

The invention relates to polyfunctional polymer networks comprising an assembly of cross-linked poylmer subchains attached to a surface, with each polymer subchain comprising a multitude of identical or different repeating units carrying one or more functional groups which allows an interaction with a sample or probe molecule.

MICRODEVICES HAVING A PREFERENTIAL AXIS OF MAGNETIZATION AND USES THEREOF

The invention relates generally to the field of moiety or molecule isolation, detection and manipulation and library synthesis. In particular, the invention provides a microdevice, which microdevice comprises: a) magnetizable substance; and b) a photorecognizable coding pattern, wherein said microdevice has a preferential axis of magnetization. Systems and methods for isolating detecting and manipulating moieties and synthesizing libraries using the microdevices are also provided.

DEVICE AND METHOD FOR THE PRODUCTION OF RADIOCHEMICAL COMPOUNDS

The invention relates to a device for producing radiochemical compounds. Said device comprises at least a reaction module, a dosing module, and a supply module. The reaction module has at least one reaction vessel that includes a closeable opening through which substances needed for the production of a predefined radiochemical compound can be introduced into the reaction vessel of the reaction module and through which the produced radiochemical compound can be removed from the reaction vessel of the reaction module. The dosing module has at least one pipetting head which can be moved relative to the supply module and the reaction module in the x, y, and z directions and also has at least one dosing unit. At least one storage tank for one of the substances needed for the production of the respective radiochemical substance is formed in the supply module.

ONE DIMENSIONAL UNICHEMO PROTECTION (UCP) IN ORGANIC SYNTHESIS

A protected template molecule and a new one-dimensional UniChemo Protection (UCP) organic synthetic method for preparing polyfunctional organic molecules is described. The synthetic method can be used with many kinds of chemical reactions and provides selective access to many functional groups in a template molecule. The method utilizes protection groups that are each composed of building block units that can be removed one by one affording a new protection group one unit shorter or exposing a functional group on the template molecule. The exposed functional group on the template molecule can react with a target group. Different target groups can be introduced into the template molecule by using protection groups containing different numbers of building block units.

UNIVERSAL READOUT FOR TARGET INDENTIFICATION USING BIOLOGICAL MICROARRAYS

A method and apparatus for implementing the method is provided. The method involves performing an indirect competitive binding assay on a microarray to identify biological or chemical targets and screen for compounds of interest. The microarray comprises a common ligand located among membrane-, lipid- or protein-associated active binding sites. The method takes advantage of known or well-characterized binding kinetics, and steric interference between biological or chemicals targets of interest and universal readout units for different binding sites within the limited confines of a microspot. The biological targets, chemicals or organisms can specifically bind to target-binding sites, while the universal readout unit binds to the ligands in the microspot.

DEVICES AND METHODS FOR CARRYING OUT CHEMICAL REACTIONS USING PHOTOGENERATED REAGENTS

Fluidic methods and devices for conducting parallel chemical reactions are disclosed. The methods are based on the use of in situ photogenerated reagents such as photogenerated acids, photogenerated bases, or any other suitable chemical compounds that produce active reagents upon light radiation. The present invention describes devices and methods for performing a large number of parallel chemical reactions without the use of a large number of valves, pumps, and other complicated fluidic components. The present invention provides microfluidic devices that contain a plurality of microscopic vessels for carrying out discrete chemical reactions. Other applications may include the preparation of microarrays of DNA and RNA oligonucleotides, peptides, oligosacchrides, phospholipids and other biopolymers on a substrate surface for assessing gene sequence information, screening for biological and chemical activities, identifying intermolecular complex formation, and determining structural features ...

METHOD OF FIXING BIOMOLECULE ON METAL SUPPORT

A solution of a nucleic acid is spotted on a metal support comprising a metal selected from among the metals of the groups I, II, III, IV, V, VI and VII in the second to seventh periods in the periodic table and transition metals or an alloy containing such a metal, and dried. Then the support is irradiated with ultraviolet rays containing a component of a wavelength of 280 nm, preferably in a dose of 100 mJ/cm2 or more, to thereby fix the nucleic acid on the support.

THIN FILM HPMP MATRIX SYSTEMS AND METHODS FOR CONSTRUCTING AND DISPLAYING LIGANDS

The invention relates to methods and systems of unhindered construction and display of tethered organic ligand molecules, and more particularly to preparation and use of thin film, substantially non-crosslinked hydrophilic polar multi-functionalized polymers (HPMPs) anchored to a variety of functionalized substrates so that the HPMP forms a thin film matrix layer providing a unique, highly hydrated, high dielectric environment equivalent to an aqueous solution, for affinity binding of Ligands (L) to Tagged Target Molecules (TTMs).

Methods and compositions for producing biopolymeric arrays

Methods and compositions are provided for producing arrays of polymeric binding agents. In the subject methods, the individual polymers of the array are synthesized using solid phase synthesis techniques on the surface of a substrate. A critical feature of the invention is that one or more locations on the substrate surface are spatially and temporally protected by a protective bubble during the synthesis protocol, where the protective bubble may be produced using any convenient bubble producing means. The bubble producing means may be a component of either a substrate or a structure separate from the substrate. Also provided are the arrays produced by the subject methods, kits for use in practicing the subject methods, and methods of using the arrays in analyte detection assays, including hybridization assays, such as gene discovery, differential gene expression and gene sequencing assays.

Microarray substrate with integrated photodetector and methods of use thereof

The present invention provides a microarray substrate comprising a plurality of photodetectors integrated therein. The invention further provides a detection device for use in conjunction with a microarray substrate of the invention, as well as methods of use of same.

Polymer-supported solution synthesis of oligosaccharides

The present invention provides improved syntheses of oligosacchairdes. In accordance with preferred embodiments, anomeric specificity in such syntheses can be attained using polymer-supported liquid synthetic design with certain novel diether linkers. The present invention also provides novel strategies for capping imcompletely glycosylated hydroxyls.

Parallel inverse chromatography methods for simultaneous evaluation of interactions between modifying agents and receptors

Methods and apparatus for the evaluation of interactions between substances using inverse chromatography are disclosed. Preferably, interactions are evaluated between a liquid test sample and a solid phase comprising a receptor in the presence of a liquid carrier. Preferably, one of the modifying agent or receptor are members of a combinatorial library.

Toxin detection and compound screening using biological membrane microarrays

A microarray containing components of cell membranes and a high-throughput method for using the microarray to detect toxins and screen for other biological or chemical compounds that may block the binding of toxin molecules to targets is provided. The microarray according to the present invention provides an attractive platform for efficient study of fundamental aspects of molecular recognition at the cell surface. Specific binding pattern of a given toxin to a set of different biological membrane probes in the microarray can be employed as a “signature” to identify and detect the presence of a toxin in a sample.

Multicomponent devices for molecular biological analysis and diagnostics

An electronic device performs active biological operations such as, for example, the analysis of a solution containing charged biological materials. The device can take the form of a flow cell that includes an inlet chamber and an outlet chamber connected to a flow cell chamber. An array containing a plurality of electrodes is contained within the flow cell chamber. Inlet and outlet ports are provided in the inlet chamber and outlet chamber, respectively. The inlet and outlet chambers advantageously have substantially constant cross-sectional flow areas.

Device for delivery of multiple liquid sample streams to a mass spectrometer

An electrospray apparatus employing multiple electrospray needles mounted in a circular arrangement sequentially delivers multiple sample streams to a mass spectrometer for analysis. One electrospray device includes an electrospray chamber, a rotatable needle supporting plate, a plurality of electrospray needles mounted on the plate, and a charger for applying a charge to droplets delivered to the electrospray chamber by the needles. Another electrospray device includes an electrospray chamber, a plurality of electrospray needles arranged in a circular arrangement, a charger, and a rotatable member for delivering gas phase ions from one needle at a time to the mass spectrometer. The rotatable electrospray apparatus provides fast repetitive analysis of simultaneously operating chromatography columns or other sample streams with a single mass spectrometer.

SUPPORT HAVING SELECTIVELY BONDING SUBSTANCE FIXED THERETO

A support carrying an immobilized selective binding substance, that the support surface has a polymer containing the structural unit represented by the following General Formula (1) in an amount of 10% or more with respect all monomer units, and a selective binding substance is immobilized on the support surface by binding to the carboxyl group formed thereon via a covalent bond: (in General Formula (1), R1, R2, and R3 each represent an alkyl or aryl group or a hydrogen atom.) ...

METHOD FOR PRODUCING PATTERNS OF PHYSICAL, CHEMICAL OR BIOCHEMICAL STRUCTURES ON CARRIERS

The invention relates to a method for producing patterns of physical, chemical and/or biochemical structures on a carrier by means of a local transfer of electrons and/or energy from the nanostructures on the carrier. According to said method, said transfer is induced by selective excitation of the plasmons in the nanostructures. The excitation of the plasmons occurs in an especially electrical manner by means of electromagnetic radiation and/or electron radiation.

Kontinuierlich arbeitendes eindimensionales Festphasenreaktor-System

Festphasenreaktor-System aus einem annähernd eindimensional geformten beweglichen 1-D-Element mit einer Querschnittsabmessung (größte Ausdehnung) im Bereich zwischen 1 μm und 1 mm, an dessen Oberflächen affine, bioaffine, substratspezifische oder substratgruppenspezifische Targetmoleküle oder stoffbindende Strukturen vorhanden sind, gekennzeichnet dadurch, dass das 1-D-Element in longitudinaler Richtung kontinuierlich durch mindestens drei örtlich voneinander getrennte Reaktionszonen bewegt wird, in denen an den Targetmolekülen oder stoffbindenden Strukturen im bewegten Zustand gleichzeitig und örtlich hintereinander Reaktionen zur Durchführung von Stoff- bzw. Informationsübertragung, Stoffbindungen, Stoffgewinnung, Stoffwandlung, Stoffreinigung oder Analyse bzw. Detektion ablaufen.

Device for producing radiochemical compounds, comprises reaction module, dosing module, and supply module, where reaction module comprises reaction vessel with opening by which substances required for producing compounds are introduced

The device comprises a reaction module (2), a dosing module, a supply module and a dispensing module, where the reaction module comprises a reaction vessel (3) with a closable opening by which substances required for producing a predetermined radiochemical compounds are introduced into the reaction vessel. The produced radiochemical compound is removed from the reaction vessel of the reaction module. The dosing module comprises a pipetting head (9), which is relatively moved to the supply and reaction modules and comprises a dosage unit (10a, 10b, 10c, 12). The device comprises a reaction module (2), a dosing module, a supply module and a dispensing module, where the reaction module comprises a reaction vessel (3) with a closable opening by which substances required for producing a predetermined radiochemical compounds are introduced into the reaction vessel. The produced radiochemical compound is removed from the reaction vessel of the reaction module. The dosing module comprises a pipetting ...

PROCEDURE FOR THE PARALLELS SYNTHESIS AND TRANSMISSION OF MOLECULES ON A SUBSTRATE

DEVICE AND PROCEDURE FOR THE AUTOMATED SYNTHESIS OF OLIGOSACCHARIDEN

PROCEDURE AND DEVICE FOR THE PRODUCTION IN HETEROGENEOUS PHASE OF MACROMOLECULES SUCH AS PEPTIDEN, POLYNUKLOTIDEN OR POLYSOCCHARIDEN.

ITSELF ADDRESSING AND JOINING MICROELECTRONIC SYSTEMS AND DEVICES FOR MOLECULAR-BIOLOGICAL ANALYSES AND DIAGNOSES

PROCEDURE FOR THE SIFTING OF A LIBRARY OF CHEMICAL COMPOUNDS

HYDROPHOBER ARTICLE WITH RASTER OF HYDROPHILIC RANGES, ITS PRODUCTION AND USE

METHOD AND DEVICE FOR PRODUCING SACCHARIDES AND SACCHARIDE ARRAYS

The present invention relates to a method and a device for producing saccharides and saccharide arrays. Said method is particularly useful for the synthesis of saccharides in parallel and of high-density saccharide arrays, such as microarrays, which are required for high-throughput screenings. 1. A method for synthesizing saccharide comprising the steps:A) providing a solid support with at least one immobilized acceptor group for reacting with a saccharide;B) delivering the saccharide onto the solid support;C) applying a vapor of a mixture of a glycosylation reagent and a solvent onto the solid support at a temperature below 20° C. in order to initiate a coupling reaction of the saccharide to the at least one immobilized acceptor group.2. The method according to claim 1 , wherein step C) is carried out at a temperature below 5° C.3. The method according to claim 1 , wherein the ratio of the solvent and the glycosylation reagent is in the range of 1:10 to 100 claim 1 ,000:1.4. The method according to claim 1 , wherein the solvent is an aprotic organic solvent selected from: methylene chloride claim 1 , chloroform claim 1 , acetonitrile claim 1 , diethyl ether claim 1 , 1 claim 1 ,4-dioxane claim 1 , methyl tert-butyl ether claim 1 , toluene and ethyl acetate.5. The method according to claim 1 , wherein the glycosylation reagent is a Lewis acid selected from: AgOTf claim 1 , BF.OEt claim 1 , trimethylsilyl trifluoromethanesulfonate claim 1 , trifluoromethanesulfonic acid claim 1 , trifluoromethanesulfonic anhydride claim 1 , lanthanoid(III) triflates claim 1 , NIS/AgOTf claim 1 , NIS/TfOH or dimethyl(methylthio)sulfonium trifluoromethanesulfonate.7. The method according to claim 1 , wherein in step B) the saccharide is a monosaccharide.8. The method according to claim 1 , further comprising step C′) between step B) and step C):C′) drying the solid support obtained in step B) under reduced pressure and/or heating.9. The method according to claim 1 , further comprising ...

Neutralization and Containment of Redox Species Produced by Circumferential Electrodes

There is disclosed an electrode array architecture employing continuous and discontinuous circumferential electrodes. There is further disclosed a process for the neutralization of acid generated at anode(s) by base generated at cathode(s) circumferentially located to each other so as to confine a region of pH change. The cathodes can be displayed as concentric rings (continuous) or as counter electrodes in a cross pattern (discontinuous). In this way reagents, such as acid, generated in a center electrode are countered (neutralized) by reagents, such as base, generated at the corners or at the outer ring. 1. An oligonucleotide bound to a semiconductor electrode array comprising:an oligonucleotide bound to a porous reaction layer adsorbed to and forming a layer on a top surface of a semiconductor electrode array, where the semiconductor electrode array comprises a plurality of continuous circumferential electrodes, where each of the plurality of continuous circumferential electrodes is separately addressable, where each electrode of the plurality of continuous circumferential electrodes comprises:(a) a top conducting surface region, where the top conducting surface region is defined by a top conducting surface which makes up a part of the top surface;(b) an insulating material in a gap region, where the gap region is defined by an inner margin and an outer margin, where the inner margin borders with the insulating material that surrounds an anode of a first continuous circumferential electrode of the plurality of continuous circumferential electrodes and the outer margin is surrounded by a cathode of the first continuous circumferential electrode, where the anode is adapted to produce an acid, and where the cathode is adapted to produce a base; and(c) the porous reaction layer formed from polysaccharide material and monosaccharide material that adsorbs to the top conducting surface and contains free hydroxyl groups or free amine groups or sulfhydryl groups (or ...

POLYMERIZED MICROARRAYS

Micropatterns of glycan-bearing brush polymers generated by the initiation of oligomerization of acrylate and methacrylate monomers from thiol-terminated surfaces. Chain lengths are controlled in situ by varying exposure time, and these multivalent glycan scaffolds detect glycan binding proteins at sub-micromolar concentrations. 1. A microarray comprising:a thiol-terminated substrate;a plurality of polymer brushes bound via thiol-(meth)acrylate polymerization to the thiol-terminated substrate.2. The microarray of claim 1 , wherein the polymer brushes exhibit a linear length growth rate from the substrate.3. The microarray of claim 1 , wherein the polymer brushes comprise a polymer grown by free radical polymerization.4. The microarray of claim 1 , wherein the polymer brushes comprise a materials selected from n (meth)acrylate oligomer and poly((meth)acrylate polymer.5. The microarray of claim 1 , wherein each of the polymer brushes comprise a plurality of binding sites for molecules selected from the group consisting of glycans claim 1 , glycan binding proteins claim 1 , antibodies claim 1 , peptides claim 1 , small molecules claim 1 , and DNA.7. The method of claim 6 , wherein the initiator is selected from the group consisting of a photoinitiator or a radical initiator.8. The method of claim 7 , wherein the initiator is a photoinitiator.9. The method of claim 8 , wherein the deposition of the (meth)acrylate-containing monomers and deposition of the photoinitiator is done simultaneously.10. The method of claim 8 , wherein the deposition of the (meth)acrylate-containing monomers and deposition of the photoinitiator is by polymer pen lithography and further wherein the acrylate-containing monomers and the photoinitiator are constituents of an ink for polymer pen lithography.11. The method of claim 8 , wherein the irradiation is by beam pen lithography.12. The method of wherein the photoinitiator is selected from the group consisting of 2 claim 8 ,2-dimethoxy-2- ...

DEVICE AND METHOD FOR THE PRODUCTION OF RADIOCHEMICAL COMPOUNDS

The invention relates to a device for the preparation of radiochemical compounds. It is provided that the device comprises at least a reaction module, a dosing module, and a storage module, wherein 1. A device for the preparation of radiochemical compounds comprising:at least a reaction module, a dosing module, and a storage module, whereinthe reaction module has at least one reaction vessel having a closable opening through which substances needed for the preparation of a predetermined radiochemical compound can be introduced into the reaction vessel of the reaction module and through which the prepared radiochemical compound can be removed from the reaction vessel of the reaction module;the dosing module has at least one pipetting head which can be moved relative to the storage module and the reaction module and in x, y, and z directions and also has one or more dosing units, wherein at least one dosing unit is a triple lumen dosing unit having a first channel for taking up, transporting, and releasing a substance needed for the synthesis of the radio chemical compound, a second channel for supplying a gas into a reaction vessel and a third channel for draining off gaseous reaction products from the reaction vessel; andat least one reservoir for one of the substances needed for the preparation of the respective radiochemical compound is formed in the storage module.2. The device according to claim 1 , wherein the reaction vessel of the reaction module has an internal volume of 10 nl to 20 claim 1 ,000 □l.3. The device according to claim 1 , wherein the reaction module has at least one of a heating claim 1 , cooling claim 1 , or facility microwave.4. The device according to claim 1 , wherein the reaction vessel has a closure with the opening being opened when a substance needed for the preparation of the respective radiochemical compound is introduced into the reaction vessel or the reaction mixture or a part of that is drained off from the reaction vessel and the ...

Method for Preparing Topographically Structured Microarrays

A method for preparing a topographically structured hydrogel microarray is described comprising the steps of a) providing one or more types of biomolecule(s) on top of micropillars of an array of micropillars, preferably by means of robotical spotting, b) providing a partially crosslinked hydrogel on a substrate, preferably attached to a substantially rigid and/or planar substrate, c) simultaneously soft-embossing a hydrogel microwell array and transferring the biomolecule(s) from the micropillars to the microwells by pressing the micropillars of the array of step a) onto the partially crosslinked layer of hydrogel of step b) until substantial completion of crosslinking and d) demolding the array of micropillars of step a) from the hydrogel microwell array of step c). The method according to the invention has the advantages of resulting in higher biochemical patterning precision, allowing for modulation of biochemical parameters by interfacing microarray manufacture with robotic technology and rendering the microarrays obtained compatible with existing read-out systems such as microscopes. Further, the elasticity of the hydrogel can be varied by tuning its shear modulus.

DUAL RUN CASSETTE FOR THE SYNTHESIS OF 18F-LABELLED COMPOUNDS

The invention provides a new chemical process, a new cassette configuration, and new software. The invention allows one synthesizer in one hot cell to produce sequentially two batches of [F]-labelled PET tracer in the same day. 1. A cassette for the synthesis of a plurality of batches of an [F]-labelled positron-emission tomography (PET) tracer wherein said cassette comprises:(i) an anion exchange column for each of said plurality of batches;(ii) a reaction vessel;(iii) a vial containing an aliquot of eluent for each of said plurality of batches;(iv) a vial containing an aliquot of a precursor compound for each of said plurality of batches;(v) reagent vials wherein each reagent vial contains an aliquot of reagent for each of said plurality of batches;(vi) optionally, a solid-phase extraction (SPE) column for deprotection and/or one or more SPE columns for purification; and,(vii) means for cleaning said reaction vessel and said SPE columns.2. The cassette as defined in wherein said PET tracer is selected from [F]fluorodeoxyglucose ([F]FDG) claim 1 , [F]Fluoromisonidazole ([F]FMISO) claim 1 , [F]fluorothymidine ([F]FLT) claim 1 , [F]Fluoroazomycin arabinofuranoside ([F]FAZA) claim 1 , [F]Fluoroethyl-choline ([F]FECH) claim 1 , [F]fluorocyclobutane-1-carboxylic acid ([F]FACBC) claim 1 , [F]flumanezil ([F]FMZ) claim 1 , [F]tyrosine claim 1 , [F]altanaserine claim 1 , 4-[F]fluoro-3-iodobenzyl guanidine ([F]FIBG) claim 1 , meta-[F]fluorobenzylguanidine ([F]mFBG) and [F]5-fluorouracil.3. (canceled)4. (canceled)5. The cassette as defined in wherein said anion exchange column is a quaternary ammonium anion exchange (QMA) column.6. The cassette as defined in wherein said eluent comprises a cationic counterion dissolved in an organic-aqueous solution.7. (canceled)8. (canceled)9. (canceled)10. (canceled)11. (canceled)12. (canceled)13. (canceled)14. (canceled)15. (canceled)16. (canceled)17. (canceled)18. The cassette as defined in wherein said means for cleaning said reaction ...

SURFACE MEDIATED SYNTHESIS OF POLYNUCLEOTIDES, POLYPEPTIDES AND POLYSACCHARIDES AND RELATED MATERIALS, METHODS AND SYSTEMS

Surface mediated polymer synthesizing methods and related systems and materials are described where monomers are attached to monomer binding regions on a surface and subsequently form chemical bonds with adjacent monomers on the surface to form linear polymers selected from polynucleotide, polypeptides and polysaccharides.

Neutralization and Containment of Redox Species Produced by Circumferential Electrodes

There is disclosed an electrode array architecture employing continuous and discontinuous circumferential electrodes. There is further disclosed a process for the neutralization of acid generated at anode(s) by base generated at cathode(s) circumferentially located to each other so as to confine a region of pH change. The cathodes can be displayed as concentric rings (continuous) or as counter electrodes in a cross pattern (discontinuous). In this way reagents, such as acid, generated in a center electrode are countered (neutralized) by reagents, such as base, generated at the corners or at the outer ring. 1. A method for electrochemical oligomer synthesis on a dense electrode array when electrode margins are 50 microns or less distance from each other , comprising:(a) providing a dense electrode array having separately addressable electrodes;(b) contacting the dense electrode array with an electrochemical deblocking solution; and(c) conducting a deblocking step by addressing each active electrode with a first current or a first voltage, where each active electrode is surrounded by at least two neighboring electrodes that are biased with a second current or a second voltage as counter electrodes.2. The method of claim 1 , where the deblocking step addresses each active electrode with the first current.3. The method of claim 1 , where the at least two neighboring electrodes include at least four neighboring electrodes.4. The method of claim 1 , where the at least two neighboring electrodes are arranged in a pattern selected from the group consisting of nearest neighbor claim 1 , neighbor electrodes claim 1 , border of neighbor electrodes claim 1 , border of nearest-neighbor claim 1 , one closest available electrode claim 1 , and electrode that is not approach.5. The method of claim 1 , where the electrochemical deblocking solution includes between:a lower limit of approximately 10% acetonitrile; andan upper limit of approximately 99% acetonitrile.6. The method of ...

METHOD FOR PREPARING TOPOGRAPHICALLY STRUCTURED MICROARRAYS

A method for preparing a topographically structured hydrogel microarray is described comprising the steps of a) providing one or more types of biomolecule(s) on top of micropillars of an array of micropillars, preferably by means of robotical spotting, b) providing a partially crosslinked hydrogel on a substrate, preferably attached to a substantially rigid and/or planar substrate, c) simultaneously soft-embossing a hydrogel microwell array and transferring the biomolecule(s) from the micropillars to the microwells by pressing the micropillars of the array of step a) onto the partially crosslinked layer of hydrogel of step b) until substantial completion of crosslinking and d) demolding the array of micropillars of step a) from the hydrogel microwell array of step c). The method according to the invention has the advantages of resulting in higher biochemical patterning precision, allowing for modulation of biochemical parameters by interfacing microarray manufacture with robotic technology and rendering the microarrays obtained compatible with existing read-out systems such as microscopes. Further, the elasticity of the hydrogel can be varied by tuning its shear modulus. 110-. (canceled)11. A microarray comprising a substrate and a hydrogel layer on said substrate , wherein the hydrogel layer is topographically structured with microwells ,wherein the hydrogel layer has a shear modulus between 1 and 100 kPa, and each of the microwells is functionalized with more than one type of biomolecules.12. A method for preparing an array of micropillars , said array of micropillars not having any metal layer deposited thereon , comprising the steps of:a) providing a photolithography mask,b) covering a substrate with a photoresist, wherein the substrate is a substantially planar and rigid silicon wafer,c) exposing the substrate to the photolithography mask of step a) for at least one cycle followed by development of the photoresist, andd) etching the substrate to obtain the ...

ELECTROCHEMICAL REACTOR TO CONTROL THE PH IN MINIATURISED DIMENSIONS

The present invention is related to an electrochemical reactor () and a microfluidic platform () comprising this reactor (), controlling pH in a closed environment, wherein this reactor () comprises at least one cell (), wherein each cell () containing at least one micro-well () able to contain a liquid and reagents and a cap () to close the said cell () and wherein the cell () further comprises at least one working electrode () producing reversible REDOX reactions. 1. An electrochemical reactor controlling pH upon a scale of at least one pH value , in a closed environment , said reactor comprising:one or more cell(s), each cell containing two connected micro-wells separated by a diffusion barrier, wherein the first micro well comprises a working electrode functionalized with reversible REDOX molecules deposited upon the working electrode and the second micro-well comprising a counter electrode, anda cap which is configured to open and close the said cell.2. The electrochemical reactor according to the claim 1 , wherein the diffusion barrier is selected from the group consisting of a channel claim 1 , a porous material or a combination of both.3. The electrochemical reactor according to claim 1 , wherein the electrode is selected from the group consisting of gold electrode claim 1 , Platinum electrode claim 1 , Carbon electrode claim 1 , and Carbon electrode recovered by a platinum film.4. The electrochemical reactor according to claim 1 , wherein the reverse REDOX molecules are selected from the group consisting of aniline claim 1 , hydroquinone claim 1 , pyrrole claim 1 , pyrrole polymers claim 1 , and a bis-aniline-cross linked nanoparticles (NP) composite.5. The electrochemical reactor according to claim 1 , wherein the counter electrode is functionalized and prepared in a deprotonated state by an external electrode.6. The electrochemical reactor according to claim 1 , wherein the cell or the cap further comprises a pH sensor.7. The electrochemical reactor of ...

GLYCAN-BASED DRUGS, THERAPIES AND BIOMARKERS

The present disclosure discloses simple and efficient glycan- or carbohydrate-based processes or methods for the rapid identification of biological markers and therapeutic targets especially glycan-related targets of infectious diseases, cancers, autoimmune diseases, allergies, inflammation, toxicity, obesity and/or other disorders of humans, animals, plants and other organisms. Therefore, novel methods and products for the diagnosis, prevention, and treatment of such diseases obtainable based on these therapeutic targets can be developed. 2: The method of claim 1 , wherein the pharmaceutical composition is in the form of a tablet claim 1 , a capsule claim 1 , a pill claim 1 , a powder claim 1 , or granules.3: The method of claim 1 , wherein the pharmaceutical composition is administered orally.4: The method of claim 1 , wherein the analog of N-acetylneuraminic acid is N-acetylneuraminic acid methyl ester.5: The method of claim 1 , wherein the analog of N-acetylneuraminic acid is a hydroxylated analog of N-acetylneuraminic acid claim 1 , a glycoconjugate of N-acetylneuraminic acid or an N-acetylneuraminic acid containing an O- or N-glycosidic linkage to an amino acid.6: The method of claim 1 , wherein administration of N-acetylneuraminic acid methyl ester treats or prevents viral diarrhea in the patient.7: The method of claim 1 , wherein administration of N-acetylneuraminic acid methyl ester treats or prevents an infectious disease caused by an influenza virus claim 1 , a Newcastle disease virus claim 1 , or a rotavirus in the patient. This application is a divisional of U.S. application Ser. No. 14/803,725, filed Jul. 20, 2015, which is a divisional of U.S. application Ser. No. 12/900,913, filed Oct. 8, 2010, now U.S. Pat. No. 9,119,866, issued Sep. 1, 2015, which claims the benefit of priority from U.S. Provisional Application No. 61/278,685, filed Oct. 9, 2009, and U.S. Provisional Application No. 61/335,415, filed Jan. 7, 2010, and which is a continuation-in- ...

Equipment and method for measuring storage reaction

An equipment and a method for measuring storage reaction in which a reaction can be processed, measured and identified efficiently and quickly. The equipment for measuring storage reaction comprises a translucent storage section having an inlet/outlet of fluid, being fixed to each fixing position where a plurality of substances to be detected, each having a specified chemical structure, are arranged at a specified interval, and being capable of storing a basic member while allowing each chemical structure to correspond to each fixing position, a section for sucking/delivering the fluid from/into the containing section through the inlet/outlet, and a measuring equipment for receiving the light from the storage basic material at the outside of the storing section under a state corresponding to the fixing position.

Biochips with surfaces coated with polysaccharide-based hydrogels

The present invention provides a substrate having a polymerized, polysaccharide-based hydrogel attached to the surface. The hydrogel can be derivatized with binding functionalities that bind analytes from a sample. The invention further provides methods of using the device and gels that are capable of selectively binding one or more analytes from a sample.

Individually addressable micro-electromagnetic unit array chips

Microdevice containing photorecognizable coding patterns and methods of using and producing the same

This invention relates generally to the field of moiety or molecule analysis, isolation, detection and manipulation and library synthesis. In particular, the invention provides a microdevice, which microdevice comprises: a) a substrate; and b) a photorecognizable coding pattern on the substrate. Preferably, the microdevice does not comprise an anodized metal surface layer. Methods and kits for isolating, detecting and manipulating moieties, and synthesizing libraries using the microdevices are also provided. The invention further provides two-dimensional optical encoders and uses thereof.

Microdevices having a preferential axis of magnetization and uses thereof

This invention relates generally to the field of moiety or molecule isolation, detection and manipulation and library synthesis. In particular, the invention provides a microdevice, which microdevice comprises: a) a magnetizable substance; and b) a photorecognizable coding pattern, wherein said microdevice has a preferential axis of magnetization. Systems and methods for isolating, detecting and manipulating moieties and synthesizing libraries using the microdevices are also provided.

Method and system for the detection of cardiac risk factors

A system for the rapid characterization of multi-cardiovascular risk factor analyte fluids, in one embodiment, includes a light source, a sensor array, and a detector. The sensor array is formed from a supporting member, in which a plurality of cavities may be formed. A series of chemically sensitive particles, in one embodiment, are positioned within the cavities. The particles may produce a signal when a receptor, coupled to the particle, interacts with the cardiovascular risk factor analyte and the particle-analyte complex is visualized using a visualization reagent. Using pattern recognition techniques, the analytes within a multi-analyte fluid may be characterized. In an embodiment, each cavity of the plurality of cavities is designed to capture and contain a specific size particle. Flexible projections may be positioned over each of the cavities to provide retention of the particles in the cavities.

可单独选通的平面结构的微电磁单元阵列芯片

本发明揭示了具有可单独选通的微电磁单元构成的阵列的电磁芯片和电磁生物芯片，以及利用这些芯片定向操纵生物分子和化学试剂等微粒和微结构的方法。

Individually addressable micro-electromagnetic unit array chips in horizontal configurations

The present invention provides electromagnetic chips and electromagnetic biochips having arrays of individually addressable micro-electromagnetic units, as well as methods of utilizing these chips for directed manipulation of micro-particles and micro-structures such as biomolecules and chemical reagents.

Methods for transport in molecular biological analysis and diagnostics

A self-addressable, self-assembling microelectronic device is designed and fabricated to actively carry out and control multi-step and multiplex molecular biological reactions in microscopic formats. These reactions include nucleic acid hybridizations, antibody/antigen reactions, diagnostics, and biopolymer synthesis. The device can be fabricated using both microlithographic and micro-machining techniques. The device can electronically control the transport and attachment of specific binding entities to specific micro-locations. The specific binding entities include molecular biological molecules such as nucleic acids and polypeptides. The device can subsequently control the transport and reaction of analytes or reactants at the addressed specific micro-locations. The device is able to concentrate analytes and reactants, remove non-specifically bound molecules, provide stringency control for DNA hybridization reactions, and improve the detection of analytes. The device can be electronically replicated.

Method for enhancing the hybridization efficiency of target nucleic acids using a self-addressable, self-assembling microelectronic device

A self-addressable, self-assembling microelectronic device is designed and fabricated to actively carry out and control multi-step and multiplex molecular biological reactions in microscopic formats. These reactions include nucleic acid hybridizations, antibody/antigen reactions, diagnostics, and biopolymer synthesis. The device can be fabricated using both microlithographic and micro-machining techniques. The device can electronically control the transport and attachment of specific binding entities to specific microlocations. The specific binding entities include molecular biological molecules such as nucleic acids and polypeptides. The device can subsequently control the transport and reaction of analytes or reactants at the addressed specific microlocations. The device is able to concentrate analytes and reactants, remove non-specifically bound molecules, provide stringency control for DNA hybridization reactions, and improve the detection of analytes. The device can be electronically replicated.

Methods for electronically-controlled enzymatic reactions

A self-addressable, self-assembling microelectronic device is designed and fabricated to actively carry out and control multi-step and multiplex molecular biological reactions in microscopic formats. These reactions include nucleic acid hybridizations, antibody/antigen reactions, diagnostics, and biopolymer synthesis. The device can be fabricated using both microlithographic and micro-machining techniques. The device can electronically control the transport and attachment of specific binding entities to specific micro-locations. The specific binding entities include molecular biological molecules such as nucleic acids and polypeptides. The device can subsequently control the transport and reaction of analytes or reactants at the addressed specific micro-locations. The device is able to concentrate analytes and reactants, remove non-specifically bound molecules, provide stringency control for DNA hybridization reactions, and improve the detection of analytes. The device can be electronically replicated.

Method and apparatus for electronic synthesis of molecular structures

A self-addressable, self-assembling microelectronic device is designed and fabricated to actively carry out and control multi-step and multiplex molecular biological reactions in microscopic formats. These reactions include nucleic acid hybridization, antibody/antigen reaction, diagnostics, and biopolymer synthesis. The device can be fabricated using both microlithographic and micro-machining techniques. The device can electronically control the transport and attachment of specific binding entities to specific micro-locations. The specific binding entities include molecular biological molecules such as nucleic acids and polypeptides. The device can subsequently control the transport and reaction of analytes or reactants at the addressed specific micro-locations. The device is able to concentrate analytes and reactants, remove non-specifically bound molecules, provide stringency control for DNA hybridization reactions, and improve the detection of analytes. The device can be electronically replicated.

Method for the electronic analysis of a sample oligonucleotide sequence

A self-addressable, self-assembling microelectronic device is designed and fabricated to actively carry out and control multi-step and multiplex molecular biological reactions in microscopic formats. These reactions include nucleic acid hybridization, antibody/antigen reaction, diagnostics, and biopolymer synthesis. The device can be fabricated using both microlithographic and micro-machining techniques. The device can electronically control the transport and attachment of specific binding entities to specific micro-locations. The specific binding entities include molecular biological molecules such as nucleic acids and polypeptides. The device can subsequently control the transport and reaction of analytes or reactants at the addressed specific micro-locations. The device is able to concentrate analytes and reactants, remove non-specifically bound molecules, provide stringency control for DNA hybridization reactions, and improve the detection of analytes. The device can be electronically replicated.

Self-addressable self-assembling microelectronic systems and devices for molecular biological analysis and diagnostics

A self-addressable, self-assembling microelectronic device is designed and fabricated to actively carry out and control multi-step and multiplex molecular biological reactions in microscopic formats. These reactions include nucleic acid hybridization, antibody/antigen reaction, diagnostics, and biopolymer synthesis. The device can be fabricated using both microlithographic and micro-machining techniques. The device can electronically control the transport and attachment of specific binding entities to specific micro-locations. The specific binding entities include molecular biological molecules such as nucleic acids and polypeptides. The device can subsequently control the transport and reaction of analytes or reactants at the addressed specific micro-locations. The device is able to concentrate analytes and reactants, remove non-specifically bound molecules, provide stringency control for DNA hybridization reactions, and improve the detection of analytes. The device can be electronically replicated.

Self-addressable self-assembling microelectronic systems and devices for molecular biological analysis and diagnostics

A self-addressable, self-assembling microelectronic device is designed and fabricated to actively carry out and control multi-step and multiplex molecular biological reactions in microscopic formats. These reactions include nucleic acid hybridization, antibody/antigen reaction, diagnostics, and biopolymer synthesis. The device can be fabricated using both microlithographic and micro-machining techniques. The device can electronically control the transport and attachment of specific binding entities to specific micro-locations. The specific binding entities include molecular biological molecules such as nucleic acids and polypeptides. The device can subsequently control the transport and reaction of analytes or reactants at the addressed specific micro-locations. The device is able to concentrate analytes and reactants, remove non-specifically bound molecules, provide stringency control for DNA hybridization reactions, and improve the detection of analytes. The device can be electronically replicated.

Self-addressable self-assembling microelectronic integrated systems, component devices, mechanisms, methods, and procedures for molecular biological analysis and diagnostics

A method for electronically stabilizing hybridization of nucleic acids bound at a test site of a microelectronic device is described. First and second negatively charged nucleic acids are provided, the second nucleic acid being bound to the test site. A zwitterionic buffer having a conductance of less than 100 mS/cm is applied to the microelectronic device. A current is applied to the test site to positively bias the test site, such that the first negatively charged nucleic acid is transported to the positively biased test site having the bound the second negatively charged nucleic acid. At the test site, the first and second negatively charged nucleic acids hybridize. The zwitterionic buffer acquires a net positive charge under influence of the current, such that the positively charged zwitterionic buffer stabilizes the hybridization by reducing the repulsion between the first and second negatively charged nucleic acids.

Self-addressable self-assembling microelectronic systems and devices for molecular biological analysis and diagnostics

A method for analyzing nucleic acid obtained from a cell sample on a platform is described. A platform having a cell selector, a nucleic acid selector, and an array of microlocations, wherein at least one microlocation has an associated capture sequence, is provided. The cell selector is contacted with a cell sample, wherein a portion of the cells remain associated with the cell selector. At least a portion of cells associated with the cell selector are lysed to release a nucleic acid sample. The nucleic acid selector is then contacted with the nucleic acid sample, such that a portion of the nucleic acid sample remains associated with the nucleic acid selector. The associated nucleic acid sample is then released from the nucleic acid selector and then is contacted with the array of microlocations, such that at least a portion of the released nucleic acid sample hybridizes with the capture sequence.

Individually addressable micro-electromagnetic unit array chips in horizontal configurations

Apparatus for studying arrays

Apparatus and methods are disclosed for conducting chemical reactions. The apparatus comprises a plurality of wells in a housing and a channel in the housing. The channel surrounds the plurality of wells and is adapted for filling with an amount of a fluid to form a convex meniscus extending above the top of the channel. In the method one or more liquid samples are placed in separate wells in a housing surface comprising a plurality of the wells. The volume of the liquid sample in each of the wells is sufficient to form a convex meniscus at the surface of each of the wells. The liquid samples are contacted with a plurality of arrays of chemical compounds. In one approach, the liquid samples are contacted with a substrate surface having a plurality of arrays of chemical compounds arranged on the substrate surface. Each of the arrays corresponds to a respective well in the housing. As a result of the contact, the substrate surface compresses each convex meniscus without cross-contact between adjacent liquid samples.

microreactor

Vorrichtung zum Transport von mindestens einer magnetischen Partikelfraktion (4) durch ein mikrofluidisches System umfassend, a1) mindestens einen mikrofluidischem Kanal (6) a2) mit einem Fluid, umfassend a3) die magnetische Partikelfraktion (4), b1) Mittel (1) zur Erzeugung einer Fluidströmung (8) b2) axial zum mikrofluidischem Kanal (6) b3) mit zwei Schaltstellungen b4) zur Erzeugung zweier Fließrichtungen der Fluidströmung (8) im mikrofluidischen Kanal (6), b5) wobei während des Betriebs der Vorrichtung stetig ein periodischer Wechsel der beiden Schaltstellungen erfolgt, c1) mindestens ein hinzu schaltbares äußeres Magnetfeld (2, 10) im mikrofluidischen Kanal (6) c2) zur temporären Fixierung der magnetischen Partikelfraktionen (4), c3) wobei das Magnetfeld nur bei einer Schaltstellung für eine Fließrichtung der Fluidströmung (8) hinzu geschaltet ist, so dass c4) daraus eine gerichtete Bewegung der magnetischen Partikelfraktion (4) im mikrofluidischen Kanal (6) resultiert. Device for transporting at least one magnetic particle fraction (4) through a microfluidic system, a1) at least one microfluidic channel (6) a2) with a fluid comprising a3) the magnetic particle fraction (4), b1) means (1) for generating a fluid flow (8) b2) axially to the microfluidic channel (6) b3) with two switch positions b4) for generating two directions of flow of the fluid flow (8) in the microfluidic channel (6), b5) wherein a constant periodic change of the two switching positions takes place during operation of the device, c1) at least one additional external magnetic field (2, 10) in the microfluidic channel (6) c2) for temporarily fixing the magnetic particle fractions (4), c3) wherein the magnetic field is connected only at a switching position for a flow direction of the fluid flow (8), so that c4) results in a directed movement of the magnetic particle fraction (4) in the microfluidic channel (6).

Method and system for the detection of cardiac risk factors

A system for the rapid characterization of multi-cardiovascular risk factor analyte fluids, in one embodiment, includes a light source, a sensor array, and a detector. The sensor array is formed from a supporting member, in which a plurality of cavities may be formed. A series of chemically sensitive particles, in one embodiment, are positioned within the cavities. The particles may produce a signal when a receptor, coupled to the particle, interacts with the cardiovascular risk factor analyte and the particle-analyte complex is visualized using a visualization reagent. Using pattern recognition techniques, the analytes within a multi-analyte fluid may be characterized. In an embodiment, each cavity of the plurality of cavities is designed to capture and contain a specific size particle. Flexible projections may be positioned over each of the cavities to provide retention of the particles in the cavities.

Combinatorial array for nucleic acid analysis

This invention relates to an array, including a universal micro-array, for the analysis of nucleic acids, such as DNA. The devices and methods of the invention can be used for identifying gene expression patterns in any organism. More specifically, all possible oligonucleotides (n-mers) necessary for the identification of gene expression patterns are synthesized. According to the invention, n is large enough to give the specificity to uniquely identify the expression pattern of each gene in an organism of interest, and is small enough that the method and device can be easily and efficiently practised and made. The invention provides a method of analyzing molecules, such as polynucleotides (e.g., DNA), by measuring the signal of an optically-detectable (e.g., fluorescent, ultraviolet, radioactive or color change) reporter associated with the molecules. In a polynucleotide analysis device according to the invention, levels of gene expression are correlated to a signal from an optically-detectable (e.g. fluorescent) reporter associated with a hybridized polynucleotide. The invention includes an algorithm and method to interpret data derived from a micro-array or other device, including techniques to decode or deconvolve potentially ambiguous signals into unambiguous or reliable gene expression data.

Methods and apparatus for characterization of polymers using multi-dimensional liquid chromatography with regular second-dimension sampling

Methods and apparatus for characterizing a polymer sample and in preferred embodiments, libraries of polymer samples, in a comprehensive, directly-coupled multi-dimensional liquid chromatography system are disclosed. The first and second dimensions are preferably high-performance liquid chromatography dimensions, such as for example, a first dimension adapted for determining composition (e.g. adapted for mobile-phase gradient elution chromatography, including reverse phase chromatography, adsorption chromatography and the like), and a second dimension adapted for determining molecular weight or particle size (e.g., adapted for size exclusion chromatography, including gel permeation chromatography).

Methods and apparatus for characterization of polymers using multi-dimensional liquid chromatography with regular second-dimension sampling

Methods and apparatus for characterizing a polymer sample and in preferred embodiments, libraries of polymer samples, in a comprehensive, directly-coupled multi-dimensional liquid chromatography system are disclosed. The first and second dimensions are preferably high-performance liquid chromatography dimensions, such as for example, a first dimension adapted for determining composition (e.g. adapted for mobile-phase gradient elution chromatography, including reverse phase chromatography, adsorption chromatography and the like), and a second dimension adapted for determining molecular weight or particle size (e.g., adapted for size exclusion chromatography, including gel permeation chromatography).

Method and apparatus for producing position addressable combinatorial libraries

Method and apparatus for producing combinatorial position-addressable libraries of different-sequence oligomers or different-substituent small molecule compounds are disclosed. The method employs massive parallel synthesis by stepwise subunit addition or substituent addition in a dense capillary-tube array. The libraries allow high throughput screening of library compounds in either solid phase or solution phase, and position-related identification of active library compounds.

Method for arranging a polymer molecule

Method for arranging a polymer molecule

System for solid phase reactions

SOL-GEL METHOD OF FUNCTIONALIZING A SURFACE OF A SOLID SUBSTRATE.

Procédé sol-gel de fonctionnalisation d'une surface d'un substrat solide en vue de pourvoir cette surface de fonctions chimiques susceptibles de réagir avec des molécules chimiques ou biologiques, en d'autres termes de fonctions chimiques susceptibles d'immobiliser, d'accrocher, de greffer des molécules biologiques ou chimiques.L'invention trouve son application par exemple dans le cadre de la préparation de capteurs chimiques et biologiques, notamment de la réalisation de puces à ADN, à oligonucléotides, à sucres, à peptides, et à petites molécules organiques. Sol-gel process for the functionalization of a surface of a solid substrate with a view to providing this surface with chemical functions capable of reacting with chemical or biological molecules, in other words with chemical functions capable of immobilizing, binding , of grafting biological or chemical molecules. The invention finds its application for example in the context of the preparation of chemical and biological sensors, in particular the production of DNA chips, oligonucleotides, sugars, peptides, and small organic molecules.

Sol-gel method for functionalization of the surface of solid substrates

Sol-gel process for the functionalisation of a surface of a solid substrate

The invention relates to a sol-gel process for the functionalisation of a surface of a solid substrate in order to provide said surface with chemical functions that can react with chemical or biological molecules, i.e. chemical functions that can immobilise, attach, graft biological or chemical molecules. The invention can be used, for example, in the preparation of chemical and biological sensors, such as for the production of chips comprising DNA, oligonucleotides, sugars, peptides and small organic molecules.

Reaction chamber

METHOD OF LOCATION OF POLYMERIC MOLECULE

ÐÎÑÑÈÉÑÊÀß ÔÅÄÅÐÀÖÈß (19) RU (11) 2005 112 193 (13) A (51) ÌÏÊ B01J 19/00 C12Q 1/68 (2006.01) (2006.01) ÔÅÄÅÐÀËÜÍÀß ÑËÓÆÁÀ ÏÎ ÈÍÒÅËËÅÊÒÓÀËÜÍÎÉ ÑÎÁÑÒÂÅÍÍÎÑÒÈ, ÏÀÒÅÍÒÀÌ È ÒÎÂÀÐÍÛÌ ÇÍÀÊÀÌ (12) ÇÀßÂÊÀ ÍÀ ÈÇÎÁÐÅÒÅÍÈÅ (21), (22) Çà âêà: 2005112193/13, 17.09.2003 (71) Çà âèòåëü(è): ÊÀËÀ×Å ÀËÅÊÑÅÉ (DE) (30) Ïðèîðèòåò: 17.09.2002 EP 02020812.0 (43) Äàòà ïóáëèêàöèè çà âêè: 20.02.2006 Áþë. ¹ 5 (86) Çà âêà PCT: EP 03/10329 (17.09.2003) (74) Ïàòåíòíûé ïîâåðåííûé: Ñàíäèãóðñêèé Îëåã Ëüâîâè÷ Àäðåñ äë ïåðåïèñêè: 191040, Ñàíêò-Ïåòåðáóðã, à/ 40, ïàò.ïîâ. Î.Ë.Ñàíäèãóðñêîìó R U Ôîðìóëà èçîáðåòåíè 1. Ìåòîä ðàñïîëîæåíè ïîëèìåðíîé ìîëåêóëû, òàêîé êàê áèîìîëåêóëà, íà ïîäëîæêå, âêëþ÷àþùèé â ñåá ñëåäóþùèå øàãè: ïðèãîòîâëåíèå ñóáñòðàòà (3), èìåþùåãî ïîâåðõíîñòü (2); ïðèãîòîâëåíèå ïîâåðõíîñòíîãî ñëî (4) íà óêàçàííîé ïîâåðõíîñòè (2) ñóáñòðàòà (3), ïðè÷åì äàííûé ñóáñòðàò (3) è óïîì íóòûé ïîâåðõíîñòíûé ñëîé (4) ñîñòàâë þò ïîäëîæêó (5); ïîìåùåíèå ïîëèìåðíîé ìîëåêóëû (1) íà äàííûé ïîâåðõíîñòíûé ñëîå (4) â ïåðâîì ïîëîæåíèè; è àäñîðáèðîâàíèå ïîëèìåðíîé ìîëåêóëû (1) íà óêàçàííîì ïîâåðõíîñòíîì ñëîå (4) ñ ïîïå÷åíèåì àäñîðáèðîâàííîãî ñîñòî íè ïîëèìåðíîé ìîëåêóëû (1) ãäå ïîëèìåðíà ìîëåêóëà (1) èìååò ïåðâóþ êîíôîðìàöèþ íà óêàçàííîì ïîâåðõíîñòíîì ñëîå (4); ãäå óêàçàííûé ïîâåðõíîñòíûé ñëîé (4) ñêîíôèãóðèðîâàí òàêèì îáðàçîì ÷òîáû äîñòè÷ü çàäàííîãî ìîëåêóë ðíîãî âçàèìîäåéñòâè ìåæäó ïîëèìåðíîé ìîëåêóëîé (1) è óêàçàííîé ïîäëîæêîé (5), ïîçâîë þùåãî ôèêñàöèþ óêàçàííîé ïîëèìåðíîé ìîëåêóëû (1) â ïåðâîé êîíôîðìàöèè è ïåðåìåùåíèå ïî êðàéíåé ìåðå ÷àñòè ïîëèìåðíîé ìîëåêóëû (1) â óïîì íóòîì àäñîðáèðîâàííîì ñîñòî íèè ïî óêàçàííîìó ïîâåðõíîñòíîìó ñëîþ (4) îòíîñèòåëüíî óïîì íóòîé ïîäëîæêè (5) ïðèìåíåíèåì âíåøíåé ñèëû. 2. Ìåòîä ïî ï.1, îòëè÷àþùèéñ òåì, ÷òî îí âêëþ÷àåò ïîñëåäóþùóþ ôèêñàöèþ ïîëèìåðíîé ìîëåêóëû (1) íà ïîâåðõíîñòíîì ñëîå (4). 3. Ìåòîä ïî ï.1, îòëè÷àþùèéñ òåì, ÷òî îí âêëþ÷àåò øàã ïî ïåðåìåùåíèþ ïîëèìåðíîé ìîëåêóëû (1) â óêàçàííîì àäñîðáèðîâàííîì ñîñòî íèè ïî äàííîìó ïîâåðõíîñòíîìó ñëîþ (4) ìàíèïóëèðîâàíèåì ...

METHOD FOR SYNTHESIZING DIFFERENT OLIGOMER COLLECTIONS.

UN METODO ESTOCASTICO GENERAL PARA SINTETIZAR AL AZAR OLIGOMEROS QUE PUEDEN USARSE PARA SINTETIZAR COMPUESTOS A CLASIFICAR CON PROPIEDADES REQUERIDAS. EL USO DE CHAPAS DE IDENTIFICACION SOBRE LOS OLIGOMEROS FACILITA LA IDENTIFICACION DE OLIGOMEROS CON LAS PROPIEDADES DESEADAS. A GENERAL STOCHASTIC METHOD FOR SYNTHESIZING RANDOM OLIGOMERS THAT CAN BE USED TO SYNTHESIZE COMPOUNDS TO BE CLASSIFIED WITH REQUIRED PROPERTIES. THE USE OF IDENTIFICATION SHEETS ON OLIGOMERS FACILITATES THE IDENTIFICATION OF OLIGOMERS WITH THE DESIRED PROPERTIES.

Modular array arrangements

The present invention pertains to modular array arrangements comprising a carrier and at least one insert for attachment to said carrier, which at least one insert may be positioned in or on said carrier in a predetermined, fixed orientation. In addition, the present invention relates to an insert having first connecting means and at least one section adapted for the application of samples, to a method of preparing a modular array arrangement and to a method for using such modular array arrangements in a screening assay.

Bioarray chip reaction apparatus and its manufacture

Agitation systems for reversibly directing fluid samples flow back and forth across a nucleic acid array, thereby promoting hybridization between targets in the fluid sample and probes on the nucleic acid array.

Microfluidic reaction carrier

Gegenstand der vorliegenden Erfindung ist ein mikrofluidischer Reaktionsträger, der eine rein fluidische oder auch eine lichtgesteuerte Synthese und Analyse von Oligomeren oder Polymeren ermöglicht. The present invention relates to a microfluidic reaction carrier which enables purely fluidic or light-controlled synthesis and analysis of oligomers or polymers.

Microfluidic chip for synthesizing radioactively labeled molecules suitable for human imaging with positron emission tomography

The invention concerns automated, inegrated microfluidic devices comprising a chemical reaction chip for performing reactions, such as the preparation of radiopharmaceutical compounds. The chip comprises an integrated microscale column and is configured for liquid flow from the column to at least one flow channel. The fluid flow is controlled by at least two on-chip valves. These double valves are configured consecutively and within proximity of 300 micrometer from each other. The reaction chamber is substantially "coinshaped", e.g. a reaction cylinder, preferably having a height diameter to height ratio greater than 3. Curved inlet and outlet channels ensure en effective elution.

Methods for determination of single nucleic acid polymorphisms using bioelectronic microchip

Methods are provided for the analysis and determination of the nature of single nucleic acid polymorphisms (SNPs) in a genetic target. In one method of this invention, the nature of the SNPs in the genetic target is determined by the steps of providing a plurality of hybridization complexes arrayed on a plurality of test sites on an electronically bioactive microchip, where the hybridization complex includes at least a nucleic acid target containing a SNP, a stabilizer probe having a sequence complementary to the target sequence and/or reporter probe, and a reporter probe having a selected sequence complementary to either the stabilizer or the same target sequence strand wherein a selected sequence of the reporter includes either a wild type nucleotide or a nucleotide corresponding to the SNP of the target. In accordance with the invention, the stabilizer, reporter and target amplicons are hybridized using electronic assistance of the microchip system such that base-stacking energies are utilized in discerning among other identifying indicators, the presence of wild type or polymorphism sequence. Applications include disease diagnostics, such as for the identification of polymorphisms in structural genes, regulatory regions, antibiotic or chemotherapeutic resistance conferring regions, or for SNPs associated with speciation or used for determination of genetic linkage.

Methods for determination of single nucleic acid polymorphisms using a bioelectronic microchip

This application includes methods for detecting single nucleotide polymorphisms (SNPs) in a sample using an electronically addressable microchip having a plurality of test sites. A sample nucleic acid is electronically biased, concentrated at, and immobilized to a test site on the microchip. A mixture comprising a first labeled probe and a second labeled probe is electronically hybridized to the sample nucleic acid to form first or second hybridized complexes. The first labeled probe is perfectly complementary to the first sample nucleic acid and the second labeled probe is complementary to the sample nucleic acid and contains a nucleotide that forms a mismatch with the nucleotide at the site of the polymorphism. The first or second hybridized complexes are detected by determining a signal intensity of the label of the first or second probe.

Selective binding substance immobilization carrier

担体の表面に選択結合性物質を固定化した選択結合性物質固定化担体であって、担体表面が、下記一般式（１）で表される構造単位を全モノマー単位の１０％以上含有しているポリマーを有し、選択結合性物質は担体表面に生成したカルボキシル基との共有結合にて固定化されていることを特徴とする選択結合性物質固定化担体である。（一般式（１）のＲ１、Ｒ２、Ｒ３は、アルキル基、アリール基もしくは水素原子を表す。） A selective binding substance-immobilized support in which a selective binding substance is immobilized on the surface of the support, wherein the support surface contains 10% or more of the structural units represented by the following general formula (1) in total monomer units: The selective binding substance-immobilized carrier is characterized in that the selective binding substance is immobilized by a covalent bond with a carboxyl group formed on the surface of the carrier. (R1, R2, and R3 in the general formula (1) represent an alkyl group, an aryl group, or a hydrogen atom.)

Methods for electronic stringency control for molecular biological analysis and diagnostics

A self-addressable, self-assembling microelectronic device is designed and fabricated to actively carry out and control multi-step and multiplex molecular biological reactions in microscopic formats. These reactions include nucleic acid hybridizations, antibody/antigen reactions, diagnostics, and biopolymer synthesis. The device can be fabricated using both microlithographic and micro-machining techniques. The device can electronically control the transport and attachment of specific binding entities to specific micro-locations. The specific binding entities include molecular biological molecules such as nucleic acids and polypeptides. The device can subsequently control the transport and reaction of analytes or reactants at the addressed specific micro-locations. The device is able to concentrate analytes and reactants, remove non-specifically bound molecules, provide stringency control for DNA hybridization reactions, and improve the detection of analytes. The device can be electronically replicated.

A substrate for microarray and a microarray having a patterned thin layer and a method for producing the substrate for microarray and the microarray

본 발명은 기판상에 패턴화된 박막층을 갖는 마이크로어레이 기판으로서, 상기 기판과 상기 박막층 물질은 서로 다른 굴절율을 가지며, 상기 패턴화된 박막층은 조사된 여기광이 상기 기판으로부터 반사되는 제1 반사광과 상기 박막층으로부터 반사되는 제2 반사광이 보강 간섭을 일으키는 두께를 갖는 스팟 영역과 조사된 여기광이 상기 기판으로부터 반사되는 제1 반사광과 상기 박막층으로부터 반사되는 제2 반사광이 상쇄 간섭을 일으키는 두께를 갖는 여백 영역을 포함하는 마이크로어레이 기판을 제공한다. The present invention provides a microarray substrate having a patterned thin film layer on a substrate, wherein the substrate and the thin film material have different refractive indices, and the patterned thin film layer comprises: first reflected light in which irradiated excitation light is reflected from the substrate; A spot area having a thickness in which the second reflected light reflected from the thin film layer causes constructive interference and a margin having a thickness in which the first reflected light reflected from the substrate and the second reflected light reflected from the thin film layer cause destructive interference. A microarray substrate comprising a region is provided. 마이크로어레이, 패턴 Microarray, pattern

Microfluidic chip capable of synthesizing radioactively labeled molecules on a scale suitable for human imaging with positron emission tomography

본 발명은 자동화된 통합 마이크로유체 장치로서 화학 반응을 위한 화학 반응 칩, 및 칩이 통합되고, 컬럼으로부터 최소한 하나의 유동 채널에 액체 유동을 위하여 구성된 미소규모의 컬럼을 포함하며, 여기서 컬럼으로의 유체 유동은 온-칩 밸브에 의하여 조절되며, 및 마이크로유체 장치 내의 유체 유동을 조절하기 위한 최소한 2개의 온-칩 밸브를 포함한다. 마이크로유체 칩, 양전자 방출 단층촬영, 인체 영상화, 방사성 표지 분자

Preparation of biopolymer arrays

A method and apparatus for fabricating an array of biopolymers on a substrate using a biopolymer or biomonomer fluid, and using a fluid dispensing head. The head has at least one jet which can dispense droplets onto a substrate, the jet including a chamber with an orifice, and including an ejector which, when activated, causes a droplet to be ejected from the orifice. The method includes positioning the head with the orifice facing the substrate. Multiple droplets of the biopolymer or biomonomer fluid are dispensed from the head orifice so as to form an array of droplets on the substrate. A gas flow is directed through a venturi which has a throat opening communicating with the dispensing head chamber. A venturi control valve which particularly communicate with an outlet of the venturi, is adjusted to alter the chamber pressure. The venturi may be driven by a source of inert anhydrous compressed gas which assists in maintaining fluid in the head isolated from moisture.

Methods and procedures for molecular biological analysis and diagnostics

A self-addressable, self-assembling microelectronic device is designed and fabricated to actively carry out and control multi-step and multiplex molecular biological reactions in microscopic formats. These reactions include nucleic acid hybridizations, antibody/antigen reactions, diagnostics, and biopolymer synthesis. The device can be fabricated using both microlithographic and micro-machining techniques. The device can electronically control the transport and attachment of specific binding entities to specific microlocations. The specific binding entities include molecular biological molecules such as nucleic acids and polypeptides. The device can subsequently control the transport and reaction of analytes or reactants at the addressed specific microlocations. The device is able to concentrate analytes and reactants, remove non-specifically bound molecules, provide stringency control for DNA hybridization reactions, and improve the detection of analytes. The device can be electronically replicated.

Biochip with surface coated with polysaccharide hydrogel

本発明は、重合した多糖系ヒドロゲルが表面に結合されている基体を提供する。ヒドロゲルはサンプル由来のアナライトと結合する結合性官能基で誘導体化することができる。本発明はさらに、サンプル由来の1種以上のアナライトと選択的に結合することができる装置およびゲルの使用方法を提供する。

High-throughput formation, identification and analysis of various solid forms

(57)【要約】 本発明は、化合物などの物質の固体形態のアレーならびにそれを迅速にスクリーニングして、特性の向上した固体形態、特には医薬を同定・確認する方法に関する。そのような特性には、改善された生物学的利用能、溶解度、安定性、送達性、ならびにプロセッシング・処理および製造上の特徴などがある。本発明は、数百〜数千個のサンプルを並行して迅速にスクリーニングする実用的かつ経費効果の高い方法に関するものである。本発明はさらに、所望の組成、粒径、晶癖または多形形態を有する結晶を製造する上で必要な条件および／または条件範囲を確認する方法を提供する。さらに別の態様において本発明は、特定の固体形態に適合する条件および／または成分組成の組、例えば特定の医薬の有利な多形に適合する条件および／または成分を同定するハイスループットな方法を提供する。

High-throughput formation, identification, and analysis of diverse solid-forms

The invention concerns arrays of solid-forms of substances, such as compound s and rapid-screening methods therefor to identify solid-forms, particularly o f pharmaceuticals, with enhanced properties. Such properties include improved bioavailability, solubility, stability, delivery, and processing and manufacturing characteristics. The invention relates to a practical and cost - effective method to rapidly screen hundreds to thousands of samples in parallel. The invention further provides methods for determining the conditions and/or ranges of conditions required to produce crystals with desired compositions, particle sizes, habits, or polymorphic forms. In a further aspect, the invention provides high-throughput methods to identify sets of conditions and/or combinations of components compatible with particular solid-forms, for example, conditions and/or components that are compatible with advantageous polymorphs of a particular pharmaceutical.</SDO AB>

Microfluidic chip capable of synthesizing radioactively labeled molecules on a scale suitable for human imaging with positron emission tomography

본 발명은 자동화된 통합 마이크로유체 장치로서 화학 반응을 위한 화학 반응 칩, 및 칩이 통합되고, 컬럼으로부터 최소한 하나의 유동 채널에 액체 유동을 위하여 구성된 미소규모의 컬럼을 포함하며, 여기서 컬럼으로의 유체 유동은 온-칩 밸브에 의하여 조절되며, 및 마이크로유체 장치 내의 유체 유동을 조절하기 위한 최소한 2개의 온-칩 밸브를 포함한다. The present invention is an automated integrated microfluidic device comprising a chemical reaction chip for a chemical reaction, and a microscale column in which the chip is integrated and configured for liquid flow from the column to at least one flow channel, wherein the fluid to the column The flow is controlled by an on-chip valve and includes at least two on-chip valves for regulating fluid flow in the microfluidic device. 마이크로유체 칩, 양전자 방출 단층촬영, 인체 영상화, 방사성 표지 분자 Microfluidic Chips, Positron Emission Tomography, Human Imaging, Radiolabelled Molecules

Automated molecular biological diagnostic system

能在微观方式上进行分子诊断、分析、多步骤和多重反应的可自编址、自装配的微电子系统。可主动控制的反应包括核酸杂交、免疫检测、临床诊断和多步骤组合的生物聚合物合成。通过输入/输出装置与用户相连的控制器接口优选地包括一个图像显示器。控制器可与电源和接口相连，接口提供到各独立微位点的选择性连接，提供极性逆转和提供到各独立电极的选择性电压或电流水平。一个用于进行DNA样品制备、杂交、检测和数据分析的组合系统将多个步骤整合到一起。带电物质优选地通过自由电场电泳进行转运。DNA复杂性降低优选地通过将DNA结合到支持物上，由诸如限制性酶等切下未结合部分，再转移切下的片段来完成。有源可编程矩阵装置包括方形矩阵结构，该结构具有扇状分布的电连接和可选的在特异性微位点下的电连接，由此产生了高度自动化的DNA诊断系统。

Polynucleotide-array assay and methods

A method of analyzing the sequence of a polynucleotide analyte. The method includes contacting the analyte with a position-addressable array of oligonucleotides, each anchored to a solid support and having a 5'proximal and 3'-distal orientation. The hybridized oligonucleotides are then extended by strand-directed polymerase, to produce labeled, extended oligonucleotides at positions of the array corresponding to sequence matches between the array oligonucleotides and analyte regions. The pattern of label in the array is used to analyze analyte sequence.

Biological molecules comprising glycosaminoglycans

The present invention relates generally to a capture device for peptides, polypeptides or proteins or other biological or synthetic molecules, alone in a sample or associated with a cell or virus, cell or virus wall or cell membrane or viral core or an extract or fraction thereof. More particularly, the present invention provides a device useful for facilitating the identification of peptides, polypeptides or proteins or other biological or synthetic molecules alone in a sample or associated with a cell or virus, cell or viral wall, cell membrane or viral core or a portion or fraction thereof immobilized to the device. In a particular embodiment, the present invention comprises a carbohydrate molecule of one or more saccharide monomers associated with a polysaccharide molecule which in turn is immobilized to a solid support useful in the capture of peptides, polypeptides or proteins or other biological or synthetic molecules alone or associated with a cell or virus, cell or viral wall or cell membrane or viral coat or an extract or fraction thereof. The capture device is useful in combination with detection assays such as immune-based detection assays including enzyme-linked immunoassays.

Spatially encoded polymer matrix

The invention relates to a spatially encoded polymer matrix in the form of a bead or a granule for combinatorial solid phase synthesis, assaying, functional proteomics and diagnostic use. Compositions of such beads or granules are also provided. Each beaded polymer matrix of the composition comprises a plurality of spatially immobilised particles. The spatial immobilisation of the particles confers on each beaded polymer matrix a “fingerprint” which enables identification of unique beads in a population of beads. The unique identification of individual beads makes it possible to perform combinatorial chemistry strategies while logging individual chemical transformation. Also provided are methods for detection of relative positions in space of particles, methods for generating matrices, methods for distance matrix determination, methods for identifying individual matrices and devices for recording and storing images of matrices.

Spatially encoded polymer matrix

Coded nucleic acid carriers

The present invention relates generally to coded solid or semi-solid nucleic acid carriers for use in multiplexing solid phase nucleic acid-based reactions. The use of coded carriers facilitates multiplexing due to the ability to deconvolute multiple nucleic acid-based events and to correlate these to particular experiments. The present invention further provides a method for identifying a nucleic acid molecule having a defined characteristic within a population of two or more different nucleic acid molecules using coded nucleic acid carriers. Conversely, the nucleic acid can be used as the code for a particular peptide, or other chemical, bound specifically to a microsphere with a specific oligonucleotide sequence. Alternatively, the method of the present invention permits screening for molecules which interact with target nucleic acid, or other, molecules. The method and the coded carriers of the present invention enable high throughput screening of nucleic acid, or other, molecules. The method may also be automated and/or controlled by computer software.

Probe carrier, probe fixing carrier and method of manufacturing the same

A carrier having indexes in a probe non-fixing region is used and solutions containing probes are applied to respective specific positions on the carrier by referring to said indexes and fixed. The position of a target compound that is specifically bonded to a probe fixed to a probe carrier manufactured by a method according to the invention can be accurately and quickly detected by referring to the indexes.

A process for producing array plate for a biomolecule comprising a hydrophilic region and a hydrophobic region

본 발명의 생물분자용 어레이 판의 제조방법은 (a) 기판 표면에 소수성 물질을 코팅시켜 소수성 막을 형성시키는 단계; (b) 코팅된 소수성 막 상에 식각 마스크를 배치시키고 패터닝시키는 단계; (c) 패터닝에 의해 노출된 소수성 막을 식각시키는 과정을 수행하여, 친수성의 결합 부위를 형성하는 단계; (d) 잔류하는 식각 마스크를 제거하는 단계; 및 (e) 소수성 막의 소수성을 복원시키는 단계를 포함하는 것을 특징으로 한다.

Viscosity control during polynucleotide synthesis

A method of fabricating an array of biopolymer probes bound to a surface of a substrate. The method includes depositing drops, at least some of which contain probe precursors, onto the substrate surface so that the probe precursors bind to the surface through a linker. This can be repeated multiple times with the probe precursor deposited in a prior cycle becomes the linker for a probe precursor deposited in a subsequent cycle, so as to form the array. The deposited drops may have a viscosity modifier or the viscosities of them may be otherwise controlled as discussed herein. Kits and compositions are further provided.

Support having a selective binding substance attached to it

Un soporte que tiene inmovilizada una sustancia de unión selectiva, donde el soporte tiene una parte con una depresión que incluye múltiples proyecciones, la superficie del soporte comprende una resina de autofluorescencia baja que tiene una intensidad de fluorescencia de 1.000 o menos, la superficie de la resina comprende grupos carboxilo formados mediante el tratamiento de la resina con un álcali o ácido, y adicionalmente se inmoviliza una sustancia de unión selectiva sobre las superficies superiores de las múltiples proyecciones de la parte con una depresión que incluye múltiples proyecciones mediante los grupos carboxilo de la resina, donde el soporte tiene formada un área plana, y la diferencia de altura entre la superficie superior plana de las proyecciones de la parte con una depresión que incluye múltiples proyecciones y la superficie del área plana es de 50 μm o menos. donde la intensidad de fluorescencia de la resina de autofluorescencia baja se determina midiendo una placa plana limpia que tiene un grosor de 1 mm utilizando un GenePix 4000B fabricado por Axon Instruments en condiciones de una longitud de onda de excitación de 532 nm, una ganancia de fotomultiplicador fijada en 700 y una potencia de láser del 33 %. A support having a selective binding substance immobilized, where the support has a portion with a depression that includes multiple projections, the surface of the support comprises a low autofluorescence resin having a fluorescence intensity of 1,000 or less, the surface of the The resin comprises carboxyl groups formed by treating the resin with an alkali or acid, and additionally a selective binding substance is immobilized on the upper surfaces of the multiple projections of the part with a depression that includes multiple projections by the carboxyl groups of the resin, where the support has a flat area formed, and the height difference between the flat upper surface of the projections of the part with a depression including multiple ...

Process for producing biochip, probe solution, and biochip

The present invention provides a process for manufacturing a biochip having high density probe regions and being resistant to contamination caused by organic materials or the like. For achieving the object, the process for manufacturing a biochip of the present invention includes a step of adhering a probe solution to a substrate so as to fix a probe onto the substrate, where the probe solution 1 contains probes and molecules having hydrophobic chains and functional groups to be adsorbed onto the substrate. When the probe solution 1 adheres to the substrate 2, a monomolecular film 3 of the molecules adsorbed onto the substrate via the functional groups is formed on the substrate, and the monomolecular film 3 suppresses spreading and bleeding of the probe solution 1 so as to provide a probe solution 4. The hydrophobic chains are fluoroalkyl chains preferably

Probe carrier, probe fixing carrier and method of manufacturing the same

A carrier having indexes in a probe non-fixing region is used and solutions containing probes are applied to respective specific positions on the carrier by referring to said indexes and fixed. The position of a target compound that is specifically bonded to a probe fixed to a probe carrier manufactured by a method according to the invention can be accurately and quickly detected by referring to the indexes.

Molecular biological diagnostic systems including electrodes

A system for performing molecular biological diagnosis, analysis and multi-step and multiplex reactions utilizes a self-addressable, self-assembling microelectronic system for actively carrying out controlled reactions in microscopic formats. These reactions include most molecular biological procedures, such as nucleic acid hybridization, antibody/antigen reaction, and clinical diagnostics. Multi-step combinatorial biopolymer synthesis may be performed. A controller interfaces with a user via input/output devices, preferably including a graphical display. Independent electronic control is achieved for the individual microlocations. In the preferred embodiment, the controller interfaces with a power supply and interface, the interface providing selective connection to the microlocations, polarity reversal, and optionally selective potential or current levels to individual electrodes. A system for performing sample preparation, hybridization and detection and data analysis integrates multiple steps within a combined system. Charged materials are transported preferably via free field electrophoresis. DNA complexity reduction is achieved preferably by binding of DNA to a support, followed by cleaving unbound materials, such as by restriction enzymes, followed by transport of the cleaved DNA fragments. Active, programmable matrix devices are formed in a variety of formats, including a square matrix pattern with fanned out electrical connections, an array having electrical connections and optionally optical connections from beneath the specific microlocations. A highly automated DNA diagnostic system results.

Reaction vessel agitation apparatus

A device and method for efficiently synthesizing diverse molecular products on substrates. A parent vessel 200 contains a suspension of substrates. The suspension is pressurized with argon and transferred to a plurality of reaction vessels 201-209 in one or more reaction vessel banks where monomer addition reactions take place. Optionally, the substrates may be tagged with a tag monomer. A vortexing motor 300 vortexes the contents of reaction vessels 201-209 during monomer addition reactions to enhance synthesis. After the desired monomer and/or tag monomer addition reaction, the suspension is pressurized with argon and transferred back to parent vessel 200 for mixing. Thereafter, the suspension may be pressurized with argon and reallocated among reaction vessels 201-209 for further synthesis.

Methods for electronic fluorescent perturbation for analysis and electronic perturbation catalysis for synthesis

Methods for electronic perturbation of fluorescence, chemilluminescence and other emissive materials provide for molecular biological analysis. In a preferred method for hybridization analysis of a sample, an electronic stringency control device is used to perform the steps of: providing the sample, a first probe with a fluorescent label and a second probe with a label under hybridization conditions on the electronic stringency control device, forming a hybridization product, subjecting the hybridization product to an electric field force, monitoring the fluorescence from the hybridization product, and analyzing the fluorescent signal. The label preferably serves as a quencher for the fluorescent label. In yet another aspect of this invention, a method for achieving electronic fluorescence perturbation on an electronic stringency control device comprising the steps of: locating a first polynucleotide and a second polynucleotide adjacent the electronic stringency control device, the first polynucleotide and second polynucleotide being complementary over at least a portion of their lengths and forming a hybridization product, the hybridization product having an associated environmental sensitive emission label, subjecting the hybridization product and label to a varying electrophoretic force, monitoring the emission from the label, and analyzing the monitored emission to determine the electronic fluorescence perturbation effect. In yet another aspect of this invention, a method is provided for electronic perturbation catalysis of substrate molecules on an electronic control device containing at least one microlocation comprising the steps of: immobilizing on the microlocation an arrangement of one or more reactive groups, exposing the reactive groups to a solution containing the substrate molecules of interest, and applying an electronic pulsing sequence which causes charge separation between the reactive groups to produce a catalytic reaction on the substrate ...

Bio-barcode based detection of target analytes

The present invention relates to screening methods, compositions, and kits for detecting for the presence or absence of one or more target analytes, e.g. biomolecules, in a sample. In particular, the present invention relates to a method that utilizes reporter oligonucleotides as biochemical barcodes for detecting multiple protein structures or other target analytes in a solution.

Method of synthesizing diverse collections of oligomers

A general stochastic method for synthesizing random oligomers can be used to synthesize compounds to screen for desired properties. The use of identification tags on the oligomers facilitates identification of oligomers with desired properties.

Synthesizing and screening molecular diversity

有效地在底物上合成不同分子产物的设备和方法。一透明容器(200)含有底物的悬浮体。将底物用氩气加压并转移至发生单体反应的一个或多个反应容器库的多个反应容器中(201-209)。选择性地，底物可用一标记单体标记。在单体加成反应期间，用旋涡马达旋流反应容器(201-209)中的内含物以促进合成。在期望的单体和/或标记单体加成反应之后，将悬浮体用氩气加压，转移回到透明容器(200)中混合。此后，可将悬浮浮体用氩气加压，并被再分配在反应容器中(201-209)用于进一步的合成。

Microfluidic devices with chemical reaction circuits

New microfluidic devices, useful for carrying out chemical reactions, are provided. The devices are adapted for on-chip solvent exchange, chemical processes requiring multiple chemical reactions, and rapid concentration of reagents.

Fabrication of finely featured devices by liquid embossing

Elastomeric stamps facilitate direct patterning of electrical, biological, chemical, and mechanical materials. A thin film of material is deposited on a substrate. The deposited material, either originally present as a liquid or subsequently liquefied, is patterned by embossing at low pressure using an elastomeric stamp having a raised pattern. The patterned liquid is then cured to form a functional layer. The deposition, embossing, and curing steps may be repeated numerous times with the same or different liquids, and in two or three dimensions. The various deposited layers may, for example, have varying electrical characteristics, interacting so as to produce an integrated electronic component.

Microfluidic chip for synthesizing radioactively labeled molecules suitable for human imaging with positron emission tomography

The invention concerns automated, inegrated microfluidic devices comprising a chemical reaction chip for performing reactions, such as the preparation of radiopharmaceutical compounds. The chip comprises an integrated microscale column and is configured for liquid flow from the column to at least one flow channel. The fluid flow is controlled by at least two on-chip valves. These double valves are configured consecutively and within proximity of 300 micrometer from each other. The reaction chamber is substantially 'coinshaped', e.g. a reaction cylinder, preferably having a height diameter to height ratio greater than 3. Curved inlet and outlet channels ensure en effective elution.

Methods of making a molecular detection chip having a metal oxide silicon field effect transistor on sidewalls of a micro-fluid channel

A molecular detection chip including a metal oxide silicon-field effect transistor (MOSFET) on sidewalls of a micro-fluid channel and a molecular detection device including the molecular detection chip are provided. A molecular detection method, particularly, qualification methods for the immobilization of molecular probes and the binding of a target sample to the molecular probes, using the molecular detection device, and a nucleic acid mutation assay device and method are also provided. The formation of the MOSFET on the sidewalls of the micro-fluid channel makes easier to highly integrate a molecular detection chip. In addition, immobilization of probes directly on the surface of a gate electrode ensures the molecular detection chip to check for the immobilization of probes and coupling of a target molecule to the probes in situ.

Methods of making a molecular detection chip having a metal oxide silicon field effect transistor on sidewalls of a micro-fluid channel

A molecular detection chip including a metal oxide silicon-field effect transistor (MOSFET) on sidewalls of a micro-fluid channel and a molecular detection device including the molecular detection chip are provided. A molecular detection method, particularly, qualification methods for the immobilization of molecular probes and the binding of a target sample to the molecular probes, using the molecular detection device, and a nucleic acid mutation assay device and method are also provided. The formation of the MOSFET on the sidewalls of the micro-fluid channel makes easier to highly integrate a molecular detection chip. In addition, immobilization of probes directly on the surface of a gate electrode ensures the molecular detection chip to check for the immobilization of probes and coupling of a target molecule to the probes in situ.

Method of synthesizing diverse collections of oligomers

A general stochastic method for synthesizing random oligomers can be used to synthesize compounds to screen for desired properties. The use of identification tags on the oligomers facilitates identification of oligomers with desired properties.

Microdevices having a preferential axis of magnetization and uses thereof

Solid phase method for analyzing a biomolecule

In a method for analyzing a biomolecule by localizing the biomolecule on a solid phase and detecting the biomolecule as a visible two-dimensional signal, the signal is detected by capturing a two-dimensional image on the solid phase with use of a scanner comprising a light source for irradiating a light on the solid phase and an image sensor for receiving a reflected light from the solid phase and capturing the two-dimensional image on the solid phase by two-dimensionally scanning the solid phase, and analyzing obtained two-dimensional image data.

Method and apparatus for chemical and biochemical reactions using photo-generated reagents

This invention provides method and apparatus for performing chemical and biochemical reactions in solution using in situ generated photo-products as reagent or co-reagent. Specifically, the method and apparatus of the present invention have applications in parallel synthesis of molecular sequence arrays on solid surfaces.

Micro-array and its manufacturing method

(57)【要約】 （修正有） 【課題】生体反応分子を含むマイクロアレイ、その利用 法、およびその製造方法を提供する。 【解決手段】それぞれ特異な反応物を封入あるいは付着 した細管あるいはロッドを作成し、切削可能な接着剤材 料をこの束に含浸させるか、切削可能な接着剤材料でこ の束を包埋するかを任意に行い、この束の構成要素すべ てがその長手方向全体に一定の構成パターンを維持して いることを確認したのち、これを薄切りしてアレイを作 製すると、同じアレイを大量に製造することができる。

Microarrays and their manufacture

Circuits for the control of output current in an electronic device for performing active biological operations

A circuit for control of an output current in a multiple unit cell array includes an array of unit cells arranged in rows and columns. Each unit cell includes a column select transistor being adapted for control by a column selector and a row select transistor being adapted for control by a row selector. The column select transistor and the row select transistor are connected together in series to each other and between an output node and a first supply. A return electrode is provided to complete the circuit.

Target plate and use thereof for improved analysis

A plate suitable for use with mass spectrometers comprising a number of target spots arranged in a surface portion of said plate making it possible to deposit small amounts of fluid at said target spots without the fluid escaping or getting mixed with fluid deposited at another target surface of the same plate. The target surfaces being arranged in a surface portion of said plate so that a base material of said plate constitutes the walls of receptacles, characterised in that the shape, size, temperature and possible agents of said receptacle facilitate evaporation of a solution in which sample molecules are suspended. Embodiments include different types of matrix and enzymes arranged at said spots, and methods for enhancing MALDI analysis efficiency.

Saccharide derivatives

Disclosed are novel saccharide derivatives which inhibit binding of toxins, such as heat-labile enterotoxin or cholera toxin, to their receptors either in vitro or in vivo. Additionally, disclosed are compounds which inhibit binding of enterovirulent organisms (e.g., bacteria, virus, fungi, and the like), such as Vibrio cholerae and enterotoxigenic strains of Escherichia coli, to their cell surface receptors.

Sensors employing combinatorial artificial receptors

本发明涉及利用组合的人工受体的传感器和传感器系统。本发明的实施方案将组合人工受体用在电磁(例如，光学)和电化学传感器中。

Chemiluminescent reading of a DNA chip, useful for detecting specific microorganisms, includes immobilizing the liquid substrate in a porous membrane on the chip surface

La présente invention porte sur une méthode pour permettre une lecture en chimiluminescence d'une puce, notamment une puce à ADN.

Devices and methods for carrying out chemical reactions using photogenerated reagents

Fluidic methods and devices for conducting parallel chemical reactions are disclosed. The methods are based on the use of in situ photogenerated reagents such as photogenerated acids, photogenerated bases, or any other suitable chemical compounds that produce active reagents upon light radiation. The present invention describes devices and methods for performing a large number of parallel chemical reactions without the use of a large number of valves, pumps and other complicated fluidic components. The present invention provides microfluidic devices that contain a plurality of microscopic vessels for carrying out discrete chemical reactions. Other applications may include the preparation of microarrays of DNA and RNA oligonucleotides, peptides, oligosacchrides, phospholipids and other biopolymers on a substrate surface for assessing gene sequence information, screening for biological and chemical activities, identifying intermolecular complex formation, and determining structural features of molecular complexes.