УСТРОЙСТВО "ОРГАН НА ИНТЕГРАЛЬНОЙ СХЕМЕ"

... 1. Автономное устройство «орган на интегральной схеме» (1), содержащее: ! (a) по меньшей мере, один резервуар для подачи среды (2); и ! (b) по меньшей мере, одну секцию роста органов (3), содержащую, по меньшей мере, одну полость для органов (4, 4а, 4b); и ! где резервуар для подачи среды (2) соединяется, по меньшей мере, с одной секцией роста органов (3) посредством микрожидкостного канала подачи (6) и, по меньшей мере, с одной полостью для органов (4, 4а, 4b). ! 2. Автономное устройство «орган на интегральной схеме» (1) по п.1, где секция роста органов (3) содержит полость для стволовых клеток (9). !3. Автономное устройство «орган на интегральной схеме» (1) по п.1, дополнительно содержащее, по меньшей мере, один резервуар для сброса среды (5), где, по меньшей мере, одна полость для органов (4, 4а, 4b) соединяется, по меньшей мере, с одним резервуаром для сброса среды (5) посредством микрожидкостного канала сброса (7). ! 4. Автономное устройство «орган на интегральной схеме» (1) по п.1 ...

Ein Schnellprüfgerät

Ein Schnellerkennungsgerät, das dadurch gekennzeichnet ist: der zylindrische Behälter (4) verfügt über eine erste Kammer (41) und einer mit der ersten Kammer (41) verbundenen quantitativen Befestigungsöffnung (44); die Basis (8) ist für den Boden des zylindrischen Behälters (4) vorgesehen, wobei diese mit einer Einsetzungsmethode montiert wird, mit dem Ziel, die Einrichtung abzudichten, wobei die Mitte zwischen Basis (8) und dem zylindrischen Behälter (4) die zweite Kammer (81) bildet, und die zweite Kammer (81) ist mit der quantitativen Befestigungsöffnung (44) verbunden; eine Stecktafel (7) ist in der zweiten Kammer (81) installiert, dabei ist auf der Stecktafel (7) ein Teststreifen montiert; der in die quantitative Befestigungsöffnung (44) passende quantitative Stecker (6), verfügt oben über eine quantitative Nute (61) und an einem Ende über ein Schlüsselloch; dazu gibt es einen Schlüssel (1), der in das Schlüsselloch passt. Bei der vorliegenden Erfindung kann der quantitative Stecker ...

Nucleic adic analyzer

Method and apparatus for oil condition monitoring

VOLUME FLOWMETER INSPECTION FIXTURE

OPTICAL EQUIPMENT IN PARTICULAR FOR THE MONITORING OF DNS POLYMERASEKETTENREAKTIONEN

EQUIPMENT AND METHOD TO THE ANALYTIC MEASUREMENT AND IMMUNE TEST

Test device

TEST DEVICE

DEVICE FOR THE REGULATION OF ANALYTEN IN SOLUTIONS

PROCEDURE FOR THE ADMISSION OF LIQUID SAMPLES FOR ANALYTIC TESTS

Compact Fluid Analysis Device And Method To Fabricate

In one aspect, a device is disclosed. In one embodiment, the device comprises: a substrate having a microfluidic component formed therein, the microfluidic component 5 configured to propagate a fluid sample via capillary force through the microfluidic component; a lid coupled to the substrate, the lid configured to at least partially cover the substrate and to enclose at least a portion of the microfluidic component, the lid including at least one waveguide configured to enable irradiating the fluid sample present in the enclosed portion of the microfluidic component with an excitation, the 10 substrate further including one or more filters configured to filter the excitation from an emission generated by the fluid sample in response to the excitation. [Figure 26] r-i 0r 0 /0 ...

History logging for samples of biological material

A method of reducing quality problems associated with patient sample collection and delivery is provided. It is possible that the method can provide a chain of custody process to better track a sample from the initial point of sample collection to the final point of sample testing. Such a method can also include providing an alert to indicate a potential problem with the quality of the diagnostic test result when a measured parameter exceeds a threshold.

Devices with optically readable liquid reservoirs

A device includes a lower reservoir surface, an upper reservoir surface, and a reservoir sidewall extending between the upper and lower reservoir surfaces which together define a reservoir. The reservoir is configured to be completely filled by a liquid such that the liquid forms a column contacting the upper reservoir surface, the lower reservoir surface, and the reservoir sidewall, with a meniscus of the liquid being outside of the reservoir. At least one of the upper reservoir surface and the lower reservoir surface is configured to transmit light.

Portable diagnostic apparatus and the method thereof

A method and a portable diagnostic apparatus (20) for detecting at least one analyte from a sample using a microfluidic cartridge (22). The portable diagnostic apparatus (20) comprises a cartridge receiving unit, a cartridge driver unit (30) and an optical unit (32). A method and an apparatus of obtaining disease prevalence information comprising at least one of the portable diagnostic apparatus (20). A method and a system for managing a network of portable diagnostic apparatuses and obtaining disease prevalence information comprising at least one of the portable diagnostic apparatus (20). A diagnostic system with multiple automated features that is capable of providing a one-step solution to near-patient clinical evaluation and diagnosis.

Slide rack determination system

A slide rack determination system for a digital slide scanning apparatus. In an embodiment, a motor drives a slide rack at a known rate toward an engagement surface. The elapsed time until engagement may be used to detect the presence or absence of the slide rack and/or the height of the slide rack. In addition, one or more sensors may detect one or more features of the slide rack, and these feature(s) may be used to determine the presence or absence of a slide rack, whether usage of the slide rack is supported or unsupported by the digital slide scanning apparatus, the orientation of the slide rack, and/or the manufacturer and/or model of the slide rack.

Apparatuses and methods for manipulating droplets on a printed circuit board

Integrated nucleic acid assays

Integrated microfluidic cartridges for nucleic acid extraction, amplification, and detection from clinical samples are disclosed. The devices are single-entry, sanitary, and disposable. The devices enable simplex or multiplex nucleic acid target detection, as for example: assay panels for multiple infectious agents, or assay panels for cancerous cell types. Methods for use of microfluidic cartridges in a fully automated, pneumatically controlled apparatus are also disclosed.

Hemostasis analyzer and method

A method and a system for quantitative or qualitative determination of a target component

The invention relates to a method and a system for quantitative or qualitative determination of a target component in a liquid sample. The method comprises i) providing a plurality of magnetic particle comprising one or more capture sites for the target component on their respective surfaces; ii) providing a plurality of fluorophores configured to bind to the capture sites of the magnetic particles; iii) bringing the liquid sample into contact with the fluorophores and the magnetic particles in a flow channel of a micro fluidic device comprising a transparent window; and iv) at least temporally immobilizing the magnetic particles adjacent to the transparent window using a magnet, emitting exciting electromagnetic beam towards the immobilized magnetic particles, reading signals emitted from fluorophores captured by the immobilized magnetic particles and performing a quantitative or qualitative determination of the target component based on the read signal. The invention also relates to a ...

A method and a system for quantitative or qualitative determination of a target component

The invention relates to a method and a system for quantitative or qualitative determination of a target component in a liquid sample. The method comprises i) providing a plurality of magnetic particle comprising one or more capture sites for the target component on their respective surfaces; ii) providing a plurality of fluorophores configured to bind to the capture sites of the magnetic particles; iii) bringing the liquid sample into contact with the fluorophores and the magnetic particles in a flow channel of a micro fluidic device comprising a transparent window; and iv) at least temporally immobilizing the magnetic particles adjacent to the transparent window using a magnet, emitting exciting electromagnetic beam towards the immobilized magnetic particles, reading signals emitted from fluorophores captured by the immobilized magnetic particles and performing a quantitative or qualitative determination of the target component based on the read signal. The invention also relates to a ...

Molecular diagnostic assay system

Improved sub-assemblies and methods of control for use in a diagnostic assay system adapted to receive an assay cartridge are provided herein. Such sub-assemblies include: a brushless DC motor, a door opening/closing mechanism and cartridge loading mechanism, a syringe and valve drive mechanism assembly, a sonication horn, a thermal control device and optical detection/excitation device. Such systems can further include a communications unit configured to wirelessly communicate with a mobile device of a user so as to receive a user input relating to functionality of the system with respect to an assay cartridge received therein and relaying a diagnostic result relating to the assay cartridge to the mobile device.

Sample processing for microscopy

In some instances, an apparatus can include a light sensitive imaging sensor having a surface to receive a fluid sample, a body to be moved relative to the light sensitive imaging sensor and having a surface to touch a portion of the fluid sample, and a carrier to move the body toward the surface of the light sensitive imaging sensor to cause the surface of the body to touch the portion of the fluid sample, so that as the surface of the body touches the portion of the fluid, the surface of the body (i) is parallel to the surface of the light sensitive imaging sensor, and (ii) settles on top of the fluid sample independently of motion of the carrier.

Assay apparatuses, methods and reagents

Apparatuses, systems, method, reagents, and kits for conducting assays as well as process for their preparation are described. The apparatuses, systems, method, reagents, and kits may be employed in conducting automated analysis in a multi-well plate assay format.

APPARATUSES AND METHODS FOR MANIPULATING DROPLETS ON A PRINTED CIRCUIT BOARD

SAMPLING SYSTEMS AND TECHNIQUES FOR DETECTION OF HAZARDOUS CONTAMINANTS

Certain aspects relate to systems and usage techniques for hazardous contamination detection devices that can utilize location-specific machine-readable information tags in conjunction with optical analysis of assays such as lateral flow assays to enable enhanced reliability and analysis of contamination detection data and/or trends. Location tags affixed to test locations and/or test sample containers provide for consistent and simplified testing workflows for reliably obtaining and storing location information in association with a large number of individual test results without requiring manual recordkeeping. Hazardous contamination detection devices can programmatically implement a two-step testing workflow.

LIQUID SENSOR ASSEMBLIES, APPARATUS, AND METHODS

Disclosed is a sensor assembly including a flow channel; two or more working electrodes located in the flow channel; and one or more reference electrodes located in the flow channel, wherein a total number of working electrodes is greater than a total number of reference electrodes. Volume in the flow channel may be minimized. Liquid testing apparatus and methods of testing test liquids are provided, as are other aspects.

ACTIVE COMPOSITE MATERIALS FOR MICRO-FLUIDIC AND NANO- FLUIDIC DEVICES

Microfluidic devices in applications such as biological and chemical sensing provide high sensitivity and exploit minute amounts of samples and reagents in solutions that are low cost, compact, portable and easily distributed to end-users, etc. Polydimethylsiloxane (PDMS) is a common material for the mechanical structure of the body, micro-channels etc. However, its inert nature and biocompatibility mean that active functionality must be added after the formation of the microfluidic structures and structural elements. It would be beneficial to provide designers of microfluidic devices with the ability to implement active elements for the detection of one or more components within a fluid or arising within a fluid from a reaction upstream using a composite comprising a mechanical component, such as the PDMS, with the active material for the active element of the microfluidic device embedded with the PDMS as a composite.

A SAMPLING DEVICE, A SYSTEM COMPRISING THE SAMPLING DEVICE AND A METHOD

A sampling device comprises a receptacle (22) having an inner material receiving space (40) defined by a side wall portion (36) and a bottom wall portion (38) and having a receptacle opening (42); and a sample container (10') being adapted to close the receptacle opening (42) when collocated with the receptacle (22), and includes: a sample receiving portion (14) which, when collocated with the receptacle (22) is located in liquid communication with the inner material receiving space (40); a sample well (18) positioned radially outside of the sample receiving portion (14) in a direction perpendicular to a longitudinal axis (A), the sample well having a well opening; a liquid passageway (16) for directing liquid from the sample receiving portion (14) towards the well opening; a container (20) having a container opening; and a liquid impermeable barrier (8'; 44) for preventing liquid entering the container opening from the sample receiving portion (14).

MICROFLUIDICS CHIP WITH SENSOR DIE CLAMPING STRUCTURES

Aspects of the embodiments are directed to a microfluidic chip that includes a plurality of openings to expose a microfluidic channel. The plurality of openings are within a recessed area of the microfluidics chip, the recess defining a surface onto which a sensor die can be clamped. The surface can include a clamping bump that contacts a membrane of a solid-state chemical sensor. The surface can also include a glue stop that, in some embodiments, can act as a spacer to prevent over-compression of the sensor die as it is clamped onto the microfluidic chip. The microfluidic chip can include a rigid structure, such as a printed circuit board, that is affixed to a top surface of the microfluidic chip. The rigid structure can include contact pads that are electrically connected to sensor electrodes on the sensor die by a wire.

VERSATILE 3D STRETCHABLE MICRO-ENVIRONMENT FOR ORGAN-ON-CHIP DEVICES FABRICATED WITH STANDARD SILICON TECHNOLOGY

The present invention is in the field of microfluidic devices produced with silicon technology wherein at least one 3D microenvironment is present, a method of producing said device using silicon based technology, and a use of said device in various applications, typically a biological cell experiment, such as a cell or organ on a chip experiment, and use o the device as a microreactor.

METHOD AND SYSTEM FOR MICROFLUIDIC PARTICLE ORIENTATION AND/OR SORTING

MULTI FUNCTION SPINNING PLATFORM

The present disclosure relates to rotatable platform for use in Lab-on-disc (LoD) applications, and LoD microfluidic device having the rotatable platform and which is suitable for analysis of a fluid sample. The present disclosure further relates to realtime monitoring of cells using a centrifugal microfluidic platform. In particular, the present disclosure relates to a mobile LoD device, which can be used in remote destinations and for point of care applications. One embodiment relates to a method for monitoring microorganisms under the constant supply of nutrients, comprising the step of inoculating the cells in a culture chamber in a rotatable microfluidic platform, rotating the platform such that liquid in a reservoir connected to or located on the platform, the liquid comprising nutrients for the cells, is constantly supplied to the cells in the culture chamber by means of shear / centrifugal force resulting from rotating the platform, and continuously monitoring the cells during rotation ...

TRANSPORT DEVICE WITH AN INNER CONTAINER

The present invention relates to a transport device (10), comprising a first section (11a) of an outer container (11) comprising a shell on the inside having the shape of a spherical cap (12a), with an inner diameter and an opening (13), wherein the opening of the spherical cap faces upwards, and an inner container (14) having an upper section (15b) a lower section (15a) and an inner hollow volume (16) defined thereby, wherein at least the lower section (15a) has a spherical shape on the outside, the outer diameter of which is smaller than the inner diameter of the spherical cap (12a) of the outer container (11). The inner container (14) is suitable to be arranged in the spherical cap (12a) of the outer container (11) in a freely pivotable fashion, and is capable of accommodating a payload (17) such as a set of multiwell plates, of microfluidic chips, or of biological samples. The inner container (14) is capable to maintaining or reestablishing, an upright position when the outer container ...

SAMPLE PROCESSING FOR MICROSCOPY

MULTIPLE SEQUENTIAL WAVELENGTH MEASUREMENT OF A LIQUID ASSAY

Analyzers and methods for making and using analyzers are described such as a method in which multiple absorption readings of a liquid assay are obtained by a photodetector using multiple light sources having respective first and second wavelengths within at least two separate and independent wavelength ranges and with each of the absorption readings taken at a separate instant of time. Using at least one processor and calibration information of the liquid assay, an amount of at least one analyte within the liquid assay using the multiple absorption readings is determined.

SAMPLE-TO-ANSWER MICROFLUIDIC SYSTEM AND METHOD INCLUDING VERTICAL MICROFLUIDIC DEVICE AND AUTOMATED ACTUATION MECHANISMS

A sample-to-answer microfluidic system and method including vertical microfluidic device and automated actuation mechanisms, such as, but not limited to, automated mechanical and/or magnetic actuation, is disclosed. In some embodiments, the sample-to-answer microfluidic system includes a vertically oriented microfluidic device and a rotating actuator in relation to the microfluidic device, and wherein the microfluidic device and the rotating actuator are operating in the XZ plane. Additionally, methods of using the sample-to-answer microfluidic system are provided.

A DEVICE AND A METHOD FOR THE REVERSIBLE IMMOBILIZATION OF BIOMOLECULES

The invention relates to a device (1) for reversibly immobilising biomolecules. The device (1) comprises a container (2, 21) which can be filled with a fluid (6) containing biomolecules, and which has an opening (23) and a valve (20). The valve (20) for the controllable out-flow of the fluid (6) can be opened and closed by a closure mechanism. Magnetic particles (3) on which the biomolecules can be immobilised, in particular can be reversibly immobilised, can be arranged freely moveably in the container (2, 21). A magnet (5), for fixing the magnetic particles (3) in the container (2, 21), is arranged on the container (2, 21), wherein the fluid (6) can be removed from the container (2, 21) through the opening (23) in the open state of the valve (20).

SYSTEMS AND DEVICES FOR HIGH-THROUGHPUT SEQUENCING WITH SEMICONDUCTOR-BASED DETECTION

In one embodiment, a sample surface of a biosensor includes pixel areas and holds a plurality of clusters during a sequence of sampling events such that the clusters are distributed unevenly over the pixel areas. In another embodiment, a biosensor has a sample surface that includes pixel areas and an array of wells overlying the pixel areas, the biosensor including two wells and two clusters per pixel area. The two wells per pixel area include a dominant well and a subordinate well. The dominant well has a larger cross section over the pixel area than the subordinate well. In another embodiment, an illumination system is coupled to a biosensor that illuminates the pixel areas (1204', 1214') with different angles of illumination (1201, 1211) during a sequence of sampling events, including, for a sampling event, illuminating each of the wells (1202, 1212) with off-axis illumination (1200A, 1200B) to produce asymmetrically illuminated well regions in each of the wells (1202, 1212).

NBC-PROTECTION AND DECONTAMINATION SYSTEM

There is provided an NBC protection and decontamination system, including an enclosure, an airlock, an airlock compartment, having a ceiling, and including a back-flow collector, an air filtration unit, a compartment inlet valve communicating with the air outlet of the enclosure, an exit valve being provided in the compartment and spaced apart from the compartment inlet valve, and an opening providing controllable passage between the enclosure and the airlock, and an opening providing controllable passage between the airlock and the outside, wherein the airlock compartment is swept by two different airflows, a first airflow originating in the enclosure and a second airflow produced by the filtration unit.

SLIDE-IN CASSETTE FOR A CUP FOR TESTING OF DRUGS OF ABUSE

A specimen cup (100) has slide-in cassette (102) hermetically sealed in a chamber (104), with a outer partition being transparent. The cassette comprised chemical test strips (106) used to provide testing of drugs of abuse or other chemical or biological substances. The cassette is designed to draw urine up from the front bottom of the cup, thereby reduces the amount of urine required to perform the test. Further the cassette is designed to form isolated test channels through the use of strategically placed vertical and horizontal bars which are hermetically sealed. The cup further comprises a spill prevention flap or float (108) and an optionally enlarged sample collection portion (110) for its operation. The windows of the test cassette are covered with transparent fluid-resistant plate to prevent urine from accidentally spill onto the strips.

DEVICE FOR DETECTING ANALYTES IN FLUID SAMPLES

The present invention provides a device (100) for detecting the presence of an analyte in a liquid sample. The device (100) has an opening (115) for introducing the liquid sample into a first chamber (301) for collecting the liquid sample, and a second chamber (601) connected to the first chamber (301) by a passageway (205) and containing a test element. The device also has a third chamber (701) connected to the second chamber (601) by a channel and containing a movable member (311) having first and second positions. The third chamber (701) is divided by the movable member (311) into first (901) and second (902) zones, and the first zone (901) has a vent hole (165). The movable member (311) is in contact with at least one wall of the third chamber (701) to prevent gas communication between the first and second zones (901, 902).

METHOD FOR PRODUCING A MEMBRANE RING OR TEST STRIP RING AND RING MAGAZINE

The invention relates to a method for producing a membrane ring or test strip ring for a diagnostic test device, wherein a) an elongated strip (26) is divided into segments (20) by cuts (24) running transverse to the longitudinal axis of the strip, b) wherein the cuts (24) are made only as far as a residual width of the strip (26), so that a material bridge (38) remains intact between segments (20) adjacent at the cuts (24); c) the strip (26) is closed by joining the ends thereof together into a ring (22), wherein the cut edges of the cut (24) running toward the material bridges (38) each enclose an acute angle (a); d) the ring (22) is placed in a support structure (12) as a membrane ring or test strip ring for the test device.

AUTOMATED ULTRA-FILTRATION WORKSTATION

Many therapeutic drugs and hormones are highly protein bound, and a plasma or serum ultra-filtrate is sometimes required to measure the unbound biologically active drugs and hormones. The present invention provides an automated ultra-filtration workstation for automatically preparing plasma or serum ultra-filtrates, without the need for centrifugation or equilibrium dialysis. The regular assays that measure total drugs and hormones in plasma or serum will provide a measurement of the free drugs and hormones, when the plasma or serum ultra-filtrate is used. The present invention also provides a pipetting tool to releasably engage a pipetting tip of a disposable ultra-filtration apparatus, the disposable ultra-filtration apparatus comprising a disposable ultra-filtration cartridge and a disposable ultra-filtration pipetting tip.

REVERSIBLE FLOW CHROMATOGRAPHIC BINDING ASSAY

Disclosed are devices and methods for performing an assay which determines t he presence or quantity of an analyte in a fluid sample by detecting binding of the analyze to at least one immobilized analyze capture reagent and washing unbound mate- rial from the immobilized analyze capture reagent. The devices and methods involve an elongated solid phase flow matrix, includ- ing capillary channels capable of driving capillary fluid movement, and further including (i) a first region adapted for receipt of the fluid sample, (ii) a second region at which the analyze capture reagent is immobilized, (iii) a third region for application of a wash reagent capable of removing unbound substances from the second region; and (iv) an absorbent reservoir of high volume capacity. The second region is positioned intermediate to the first region a nd the third region and intermediate to the absorbent reservoir and the third region. The device also includes means to detect analyze bound at the second region ...

METHOD FOR COLLECTING SAMPLES OF LIQUID SPECIMENS FOR ANALYTICAL TESTING

A sample kit and a method for collecting a sample of a liquid specimen for analytical testing comprises a sample container and a reagent vial. The sample containe r includes an open end and a capillary end with a chamber disposed therebetween which includes analytical testing strips and the like within the chamber. The reagent vial is provided with a penetrable foil seal over an open end and a reagent therein for receipt of the capillary end of the sample container. The method for collecting a sample of a liquid specimen for analytical testing includes the steps of bringing the capillary end in conta ct with the liquid specimen to be analyzed and then penetrating the penetrable foil seal over t he open end of the reagent vial wherein the sample container fits within the opening in the penetrable foil seal in an air-tight manner forcing the reagent within the reagent vial into the chamber in the sample container and thereby in contact with the analytical testing strips.

Testvorrichtung

Test cutlery, consisting of a Blister, and its use.

Measuring instrument and procedure for determining fluid parameters made available by a Laborsystem.

Betrifft ein Messgerät (3) und ein Verfahren zum Bestimmen von durch ein Laborsystem (1), insbesondere ein Hybridisierungssystem (2) bereitgestellten Fluidparametern. Dabei umfasst das Messgerät (3) eine Messeinheit (5) und eine Verarbeitungseinheit zum Bestimmen der durch das Laborsystem (1) bereitgestellten Fluidparameter, und die Messeinheit (5) ist in dieses Laborsystem (1) integrierbar ausgebildet. Das erfindungsgemässe Messgerät ist dadurch gekennzeichnet, dass die Messeinheit (5) einen Messblock ohne Reaktionsraum und zumindest einen Sensor (17) umfasst; dass der Messblock Hohlräume (16) zur Aufnahme oder Leitung von durch das Laborsystem (1) bereitgestellten Fluiden (13) umfasst, wobei die Hohlräume (16) im Wesentlichen vollständig innerhalb des Messblocks angeordnet sind; und dass der zumindest eine Sensor (17), zum Bestimmen von physikalischen und/oder chemischen Parametern von sich in den Hohlräumen (16) befindlichen Fluiden (13), an oder in fluidischer Wirkverbindung mit diesen ...

Pipette und Pipettierhilfe mit 2-Zeichen-Codierung.

Dargestellt und beschrieben ist eine Pipette mit einer ersten Öffnung zur Aufnahme und Entnahme einer zu dispensierenden Flüssigkeit und einer der ersten Öffnung gegenüberliegenden zweiten Öffnung. Die Pipette (11) weist zwischen den beiden Öffnungen ein Füllvolumen auf, wobei eine Mantelfläche das Füllvolumen umgibt. Erfindungsgemäss ist vorgesehen, dass die Pipette ein Material umfasst, welches eine Verschiebung der Wellenlänge zwischen der von der Pipette absorbierten und emittierten elektromagnetischen Strahlung verursacht. Des Weiteren ist eine Pipettierhilfe (23) zur Aufnahme einer Pipette (11) für die Dispensierung einer Flüssigkeit beschrieben. Die Pipettierhilfe (23) umfasst eine Aufnahmevorrichtung zum Fixieren der Pipette (11) an der Pipettierhilfe (23), eine Betätigungsvorrichtung, deren Betätigung zur Aufnahme oder Abgabe der Flüssigkeit führt, einen Griff (27) zum Umgreifen der Pipettierhilfe (23), mindestens ein am Griff (27) angeordnetes Bedienelement zur Steuerung der Aufnahme ...

Antriebs-Modul sowie Bürette für eine Dosiereinheit sowie Dosiereinheit.

Eine Dosiereinheit umfasst ein Antriebs-Modul und eine Bürette (3), wobei die Bürette (3) in der Bürettenaufnahme angeordnet ist. Das Antriebs-Modul umfasst eine Bürettenaufnahme zur Aufnahme einer austauschbare Bürette (3). Die Bürette (3) weist einen Kolben und mindestens einen Fluidausgang auf. Das Antriebs-Modul umfasst einen Kolbenantrieb und einen Sensor. Der Sensor erfasst die Position des Kolbens und/oder des Fluidausgangs. Die Bürette (3) umfasst ein Aussengehäuse (33), ein gegenüber dem Aussengehäuse (33) um eine Drehachse drehbar gelagertes Innengehäuse, einen gegenüber dem Innengehäuse (35) drehgesicherter Flüssigkeitszylinder und einen gegenüber dem Aussengehäuse (33) drehgesicherter Deckel (38). Der Deckel (38) weist mehrere Fluidausgänge (32) auf. Durch eine Drehung des Innengehäuses (35) gegenüber dem Aussengehäuse (33) kann die Position der Fluidausgänge (32) geändert werden und auf diese Art eingestellt werden, durch welchen der mehreren Fluidausgänge (32) ein Fluid durch ...

An apparatus for performing thermal cycles in RT-PCR process

IMPROVED SPECIMEN COLLECTION AND STORAGE DEVICES AND METHODS RELATING THERETO

Rotatable test element

The invention relates to an essentially disk-shaped, flat test element that can be rotated about a preferably central axis which is perpendicular to the plane of the disk-shaped test element. Said test element contains a sample feeding opening for introducing a liquid sample, a capillary-active region, especially a porous, absorbent matrix, having a first end at a distance from the axis and a second end which is close to the axis, and a sample channel which reaches from a region close to the axis to the first end of the capillary-active region, at a distance from the axis. The invention also relates to a method for determining an analyte by means of the test element.

SORTING EQUIPMENT FOR PARTICLES IN A FLUID SAMPLE

DETECTION DEVICE, STORAGE AND DISPOSITION OF BLADES SAMPLED.

Device for storing and traceability of biological sample holders

APPARATUS OF THERMAL VALIDATION, TOGETHER Of a DEVICE OF TREATMENT Of BIOLOGICAL SAMPLES AND Of SUCH an APPARATUS, AND MANUFACTORING PROCESS Of SUCH an APPARATUS.

L'appareil de validation thermique (302) comprend au moins une chemise (316), chaque chemise délimitant un puits (314) et étant destinée à être insérée dans une cavité respective du dispositif de traitement thermique, destinée à chauffer ou refroidir des échantillons biologiques, et une sonde de température respective placée dans chaque puits (314). Chaque chemise est en matière plastique.

PACKAGING FOR COLLECTING IMPLEMENT BODILY SECRETIONS

PACKAGING FOR COLLECTING IMPLEMENT BODILY SECRETIONS

PACKAGING FOR COLLECTING IMPLEMENT BODILY SECRETIONS

SAMPLE COLLECTOR AND TEST DEVICE

CANTILEVER SENSOR TYPE ANALYSIS SYSTEM COMPRISING CANTILEVER USING PIEZOELECTRIC FILM, METHOD FOR FABRICATING THE SAME AND METHOD FOR DETECTING MICRO-ELEMENT USING THE SAME

PURPOSE: To provide a cantilever sensor type of analysis system which is able to detect micro-element in fluid as well as the air, thereby capable of detecting continuously a reaction amount, a method for fabricating the same, and a method for detecting micro-element using the same. CONSTITUTION: The system comprises a micro-fluid transfer system (100) which includes inlets (111a,111b) for injecting reactants into the system and micro-flow channels (112a,112b) connecting the inlets with a reaction chamber (115), and a cantilever sensor (200) including a cantilever, a driving film which is laminated on one side of the cantilever and consists of a piezoelectric film, an upper electrode and a lower electrode, and an electric pad applying electricity to the lower and upper electrodes. © KIPO 2006 ...

APPARATUS AND METHOD FOR DETECTING AN ANALYTE

An apparatus for detecting an analyte in a sample of material is disclosed. The apparatus includes a frame and a plurality of chambers. The frame may be manipulated to form at least a first flow path and a second flow path through the chambers. © KIPO & WIPO 2008 ...

핵산 정제 카트리지

... 하기와 같이, 복잡한 생물학적 샘플로부터 생물학적 또는 화학적 분석물을 정제하기 위한 필터를 함유하는 둘러싸인 챔버를 가지며, 상기 챔버는 상기 필터 이외에도 복수 개의 포트를 수용하는 것인 마이크로유체 장치가 개시되어 있다: 제1 유로를 통한, 챔버의 진공 발생기와의 기체 연통을 가능하게 하는 제1 포트; 제2 유로를 통한, 챔버의 하나 이상의 저장소와의 액체 연통을 가능하게 하는 제2 포트; 제3 유로를 통한, 챔버의 하나 이상의 수용 용기 및 진공 발생기 양자 모두와의 기체 및 액체 연통을 가능하게 하는 제3 포트; 및 제1 및/또는 제2 포트를 통해 챔버에 들어가고 제3 포트를 통해 챔버에서 나오는 유체가 필터를 통과해 흐르도록, 제3 포트와 제1 및 제2 포트 양자 모두의 사이에 위치하는 필터. 본 발명은 또한 마이크로유체 장치를 사용하는 방법에 관한 것이다. [대표도] 도 2a - 도 2e ...

DEVICE FOR BLOOD TEST

APPARATUS WITH ACTUATING SENSOR MODULE

MINIATURIZED IN VITRO PROTEIN EXPRESSION ARRAY

Detection of inhibition of biological synthesis of pre-characterized proteins indicates presence of a toxic analyte. The sensor array comprises an array of units; each unit is for expression of one protein and thus functions as one sensor. The sensor array expresses a group of pre-characterized proteins in different expression system, thus the response pattern (or signature) of an analyte due to different inhibitory effects is registered and used as a tool for detection and identification. New agents are identified by comparing the response pattern with signatures of known agents in a pre-collected database. The protein synthesis array device also functions as surrogate cells, which can be used for systematic and quantitative studies of the interplay among a large number of the constituents in a biological cell, for studying molecular responses to toxins, and for search for the therapeutic targets.

BLOOD COLLECTION DEVICE, METHOD, AND SYSTEM FOR USING THE SAME

Disclosed is a fluid collection device wherein multiple, individual samples of fluid can be collected simultaneously. The device includes a chamber and an adapter which substantially and simultaneously distributes the blood to individual chambers with chamber specific additives. Also included is a system for using the blood collection device, preferably within a diagnostic testing laboratory.

MICROFLUIDICS APPARATUS AND METHODS

This invention relates to microfluidics apparatus and methods for particle concentration in sensors for sensing biological entities such as cells, spores and the like. We describe a microfluidic sensor for sensing biological particles including a particle concentration device for performing concentration of particles in three dimensions. The sensor device comprises a substrate bearing a microfluidic channel or chamber for carrying a conductive fluid bearing the particles. The channel has: first and second electrodes spaced apart on the channel or chamber for defining an electric field therebetween, and a sensing surface on an inner surface of the channel or chamber. The particle concentration device comprises means for applying an ac voltage across the electrodes to perform simultaneously: i) electrohydrodynamic generation of a convection current flow in the fluid; and ii) 3D concentration of the particles in said fluid by dielectrophoretic attraction or repulsion of the particles towards ...

MULTI-DIMENSIONAL FLUID SENSORS AND RELATED DETECTORS AND METHODS

The present invention provides multi-dimensional sensors with fluidic flow channels for processing fluid samples.

THERMAL SENSOR FOR FLUID DETECTION

Embodiments of the invention provide a method for detecting the arrival of a fluid at or near a thermal sensor. A method includes obtaining a signal from the thermal sensor prior to arrival of the fluid, obtaining another signal during arrival of the fluid, and relating the two signals to indicate the arrival of the fluid. Devices for application of the method are also described.

Compact sanitary urinalysis unit

The disclosure relates to a device for performing routine urinalysis. A closed integrated system enables the user to do the important variety of tests and steps during each procedure, thereby greatly minimizing the exposure of the user to the undesired contamination from the urine specimen being tested and facilitating the performance of the testing procedure under a shorter timeframe and initiating lesser laboratory wares expenditure. The device is comprised of a clear and transparent tubular container, subdivided into a larger main longitudinal chamber integrated with two adjacent smaller longitudinal chambers therein. One of the smaller longitudinal chambers being calibrated to the equivalence of the routinely accepted specific gravity urinometer (hydrometer) and, inside this chamber is a small flotation component for determining the specific gravity of the urine specimen trapped therein. The second smaller longitudinal chamber contains a built-in chemical reagent strip so that upon ...

Method and apparatus for penetrating tissue

A tissue penetration device includes a penetrating member driver, a cartridge, and a plurality of penetrating members integrated with the cartridge. Each of a penetrating member is coupled to the penetrating member driver when advanced along a path into a tissue target. A user interface is configured to relay at least one of, skin penetrating performance or a skin penetrating setting.

Clinical array assays that include a sample quality evaluation step and compositions for use in practicing the same

Array-based clinical assays and compositions for use in practicing the same are provided. A feature of the subject array-based clinical assays is that they include a sample quality evaluation step that is independent from the clinical assay step of the assays, where the sample quality evaluation step may be performed in a number of different ways. Also provided are compositions, devices and kits for use in practicing the subject methods.

Reversible flow chromatographic binding assay system, kit, and method

Disclosed are assay systems, kits, and methods for performing an assay which determines the presence or quantity of an analyte in a fluid sample by detecting binding of the analyte to at least one immobilized analyte capture reagent and washing unbound material from the immobilized analyte capture reagent. An elongated solid phase flow matrix includes: (i) a first region adapted for receipt of the fluid sample, (ii) a second region at which the analyte capture reagent is immobilized, (iii) a third region for application of a liquid wash and/or detector reagent capable of removing unbound substances from the second region; and (iv) an absorbent reservoir of high volume capacity. The second region is positioned between the first region and the third region and between the absorbent reservoir and the third region. The device also includes means to detect analyte bound at the second region. The fluid sample flows by capillary action initially along the elongated flow matrix in one direction ...

Method and apparatus for detecting insoluable constituents in a quiescent urine sample

A urine sample is analyzed for urine chemistry, formed bodies, and rare event evidence, all in a single sample container and under low power magnification. The sample container includes a urine sample receiving chamber which is connected to a urine chemistry chamber, to a formed body isolation chamber, and to a rare events detection chamber, so that the urine can flow from the receiving chamber to the other three chambers. The scanning instrument will scan the isolation chamber and can identify formed bodies by their characteristic light wave emission properties which result from the formed bodies exposure to the stains. The formed bodies can also be morphologically examined in the isolation chamber. The rare event detection chamber will include a component which will absorb essentially all of the water in the urine thus concentrating formed bodies on a surface in the chamber. This chamber can also be provided with one or more stains which will differentially highlight any rare events noted ...

OPTOFLUIDIC ANALYTE DETECTION SYSTEMS USING MULTI-MODE INTERFERENCE WAVEGUIDES

Systems, methods, and techniques for optofluidic analyte detection and analysis using multi-mode interference (MMI) waveguides are disclosed herein. In some embodiments, spatially and spectrally multiplexed optical detection of particles is implemented on an optofluidic platform comprising multiple analyte channels intersecting a single MMI waveguide. In some embodiments, multi-stage photonic structures including a first stage MMI waveguide for demultiplexing optical signals by spatially separating different wavelengths of light from one another may be implemented. In some embodiments, a second stage may use single-mode waveguides and/or MMI waveguides to create multi-spot patterns using the demultiplexed, spatially separated light output from the first stage. In some embodiments, liquid-core MMI (LC-MMI) waveguides that are tunable by replacing a liquid core, heating/cooling the liquid core, and/or deforming the LC-MMI to change its width may be implemented in one or more of the analyte detection/analysis systems disclosed herein. 1. A system for on-chip analyte detection , comprising:a substrate;a first analyte channel, disposed on the substrate, configured to receive a first liquid containing first analytes to be detected by the system;a second analyte channel, disposed on the substrate, configured to receive a second liquid containing second analytes to be detected by the system; receive input light of a first wavelength and input light of a second wavelength;', 'generate a first spot pattern having a first number of spots of light of the first wavelength incident on the first analyte channel;', 'generate a second spot pattern having a second number of spots of light of the first wavelength incident on the second analyte channel;', 'generate a third spot pattern having a third number of spots of light of the second wavelength incident on the first analyte channel; and', 'generate a fourth spot pattern having a fourth number of spots of light of the second ...

Device and method for analyzing total cyanide in water samples

A device and a method of analyzing total cyanide in water samples, including a mixing tube, a stripping tube, a sampling pipe, a reaction pipe, a peristaltic pump, an air pump, a UV digester, a heater, a condenser, a drain pipe and a water sample detector. The mixing tube, the stripping tube, the sampling pipe and the reaction pipe are respectively connected to the peristaltic pump. The mixing tube is connected to the reaction pipe. A bottom of the stripping tube, the air pump, the UV digester, the heater and the condenser and are sequentially connected. The condenser is connected to the mixing tube. An upper portion of the stripping tube, the sampling pipe and the reaction pipe are connected to the drain pipe. An absorbance of the water in the mixing tube is measured by the sample detector, and used to calculate the total cyanide content by spectrophotometry.

SYSTEMS AND METHODS FOR AFFINITY CAPILLARY ELECTROPHORESIS

The presently disclosed subject matter relates to compositions, systems and methods of screening one or more species of polypeptide in a complex mixture of polypeptides, e.g., multi-subunit proteins. For example, the subject matter relates to ligands used in connection with affinity capillary electrophoresis, as well as methods and systems for detecting polypeptides in a mixture of multimers that include multispecific antibodies, e.g., bispecific antibodies.

CARTRIDGES, KITS, AND METHODS FOR ENHANCED DETECTION AND QUANTIFICATION OF ANALYTES

Devices, systems, and methods for detecting molecules of interest within a collected sample are described herein. In certain embodiments, self-contained sample analysis systems are disclosed, which include a reusable reader component, a disposable cartridge component, and a disposable sample collection component. The reader component may communicate with a remote computing device for the digital transmission of test protocols and test results. In various disclosed embodiments, the systems, components, and methods are configured to identify the presence, absence, and/or quantity of particular nucleic acids, proteins, or other analytes of interest, for example, in order to test for the presence of one or more pathogens or contaminants in a sample.

Pressure-variation fluid transport, in particular for body-fluid analysis

Disclosed is the sensing a sample fluid. The sample fluid is first provided into a cartridge (400), and the cartridge (400) inserted into a reading device (420). A pressure variation is provided in the cartridge (400), and the sample fluid will be moved to a sensing element (140) by using the provided pressure variation and by controlling the timing for releasing a pressure in the pressure variation means (40, 50, 100, 110). The moved the sample fluid can then be sensed by means of the sensing element (140).

SUBSTRATES WITH INDEPENDENTLY TUNABLE TOPOGRAPHIES AND CHEMISTRIES FOR QUANTIFIABLE SURFACE-INDUCED CELL BEHAVIOR

A method for measuring surface-induced cellular behavior that includes one or more lithographically patterned, functionalizable structures on a substrate, for example gold islands or grooved quartz, in contact with a fluid and in registry with at least one living cell for a plurality of times. The structures' shape, height, pitch and ordering are controlled by the lithographic process, such that the physical cues imparted to the cell by topography can be tuned independently of the chemical biofunctionality which is subsequently imparted via surface chemistry. Cellular behavior data, such as adhesion, migration, differentiation, division, secretion, apoptosis and necrosis, is measured using imaging sensors in relation to the surface topography and surface chemistry for a plurality of times.

LABORATORY STOPCOCK VALVE MANIFOLD WITH ARBITRARY MAPPING OF FLOW TO ROTATION ANGLE AND PROVISIONS FOR MOTORIZED PLANETRAY GEAR CONTROL

An article of laboratory glassware for directing the flow of chemical materials is described. The article includes a glass manifold having a plurality of input ports and at least one output port, and a plurality of stopcocks. Each stopcock has an inlet port and an outlet port connected by a passageway through the plug. Each of the stopcock output ports is connected to one of the manifold input ports, and each stopcock input port is connected with one end of a hollow glass tube, and the other end of the hollow glass tube is connected to a ground glass joint. The output ports of the manifold are terminated to a ground glass joint. Each plug is rotationally coupled to a planetary gear arrangement operated by a stepper or electrical motor, and rotational position can be sensed by a sensor. The rotation of each rotating plug is controlled by a computer.

SYSTEMS AND METHODS FOR OPTICALLY PROCESSING SAMPLES

A system for processing a sample includes a chamber having at least one inlet and at least one outlet, where the chamber is configured to accommodate flow of the sample from the at least one inlet toward the at least one outlet, and an imager array configured to image the flow of the sample in the chamber, where the imager array includes at least one lensless image sensor configurable opposite at least one light source.

PARTICLE SEPARATOR SYSTEM, MATERIALS, AND METHODS OF USE

The present invention concerns systems, materials, and methods of cell and particle separation utilizing magnetic levitation to affect separation.

Reduced Dimensionality Structured Illumination Microscopy With Patterned Arrays of Nanowells

Techniques are described for reducing the number of angles needed in structured illumination imaging of biological samples through the use of patterned flowcells, where nanowells of the patterned flowcells are arranged in, e.g., a square array, or an asymmetrical array. Accordingly, the number of images needed to resolve details of the biological samples is reduced. Techniques are also described for combining structured illumination imaging with line scanning using the patterned flowcells.

Fluidic channels including conductivity sensor and methods of use thereof

Devices that includes a first portion, the first portion including at least one fluid channel; a fluid actuator; an analysis sensor disposed within the fluid channel; a conductivity sensor disposed within the fluid channel; and an introducer; a second portion, the second portion comprising: at least one well, the well containing at least one material, wherein one of the first or second portion is moveable with respect to the other, wherein the introducer is configured to obtain at least a portion of the material from the at least one well and deliver it to the fluid channel, and wherein the fluid actuator is configured to move at least a portion of the material in the fluid channel.

DIELECTRIC SENSING TO CHARACTERIZE HEMOSTATIC DYSFUNCTION

As one example, an apparatus includes a dielectric microsensor having a microfluidic chamber that includes a capacitive sensing structure. The microfluidic chamber is adapted to receive a volume of a blood sample, and a bioactive agent is adapted to interact with the blood sample, as a sample under test (SUT), within the microfluidic chamber. A transmitter can provide an input radio frequency (RF) signal to the dielectric microsensor, and a receiver can receive an output RF signal from the dielectric microsensor. A computing device is configured to compute dielectric permittivity values for the SUT based on the output RF signal over a time interval in which the dielectric permittivity values are representative of the bioactive agent interacting with the blood sample over the time interval. The computing device is further configured to provide a readout representative of hemostatic dysfunction for the blood sample based on the dielectric permittivity values.

MANUFACTURING PROCEDURE FOR LABORATORY INTEGRATED ON A CHIP

流体試料の収集および試験装置

... 流体試料を集めて試験するためのデバイスを提供する。このデバイスは、コレクション・チャンバー、隔離チャンバーおよび試験チャンバーを含む。コレクション・チャンバーは、隔離チャンバーと流体連絡状態にあり、試験チャンバーに対して封止されている。また、隔離チャンバーは、試験チャンバーに対して封止されている。流体試料は、コレクション・チャンバー内に集められ、アリコートが隔離チャンバーにおいて隔離される。隔離チャンバーから流体を放出して、コレクション・チャンバーに対して隔離チャンバーを封止しながら、試験チャンバーへの流れパスを形成するように、流体放出要素が供給されている。本発明のもう1つの要旨では、試料コンテナは、それが開けられたか否かの識別を行う、従って、コンテナの内容物が汚染されたということを示すタンパー・エビデントな蓋を含んでよい。 ...

СОСУД ДЛЯ ТЕСТИРОВАНИЯ, ИНДИКАТОРНАЯ ПОЛОСКА, НАБОР ДЛЯ ТЕСТИРОВАНИЯ И СПОСОБ ТЕСТИРОВНИЯ

Группа изобретений относится к сосуду для тестирования, используемому в способе тестирования, имеющем стадию погружения одного конца индикаторной полоски в образец текучей среды, а также к набору для тестирования, включающему индикаторную полоску и указанный сосуд для тестирования. Сосуд для тестирования включает корпус сосуда, имеющий отверстие в его верхнем конце, а также емкость для хранения, сообщающуюся с отверстием, и в которой хранится образец текучей среды. Сосуд также включает крышку сосуда, которая герметично закрывает отверстие корпуса сосуда с возможностью прикрепления/снятия, и участок, фиксирующий индикаторную полоску. Указанный участок предоставлен в крышке корпуса и фиксирует другой конец индикаторной полоски с возможностью прикрепления/снятия, а также удерживает индикаторную полоску в емкости для хранения в то время, когда отверстие корпуса сосуда герметично закрывают крышкой корпуса. При этом крышка корпуса образована из колпачка, подогнанного к отверстию корпуса сосуда ...

СЕКВЕНИРОВАНИЕ С ВЫСОКОЙ ПРОПУСКНОЙ СПОСОБНОСТЬЮ С ДЕТЕКТИРОВАНИЕМ НА ОСНОВЕ ПОЛУПРОВОДНИКА

Изобретение относится к секвенированию с детектированием на основе КМОП и, более конкретно, к системам и способам для повышения пропускной способности секвенирования с помощью детектирования на основе КМОП. Биодатчик для определения оснований в последовательности нуклеиновой кислоты содержит устройство анализа образца, которое содержит поверхность для образцов, имеющую массив областей пикселей, и твердотельный формирователь изображения, имеющий матрицу датчиков, причем каждый датчик формирует сигналы пикселей в каждом цикле определения оснований, каждый сигнал пикселя представляет свет, получаемый в одном цикле определения оснований из одного или более кластеров в соответствующей области пикселя поверхности для образцов; и процессор сигналов, выполненный с возможностью соединения с устройством анализа образцов, которое принимает и обрабатывает сигналы пикселей с датчиков для определения оснований в цикле определения оснований и использует сигналы пикселей из меньшего числа датчиков, чем ...

МИКРОСКОПИЯ СТРУКТУРИРОВАННОГО ОСВЕЩЕНИЯ УМЕНЬШЕННОЙ РАЗМЕРНОСТИ СО СТРУКТУРИРОВАННЫМИ МАССИВАМИ НАНОРАЗМЕРНЫХ ЛУНОК

Изобретение относится к области микроскопии структурированного освещения (SIM). Технический результат заключается в уменьшении числа изображений и размеров, необходимых, чтобы разрешать флуоресцентные образцы с использованием SIM с помощью структурированных особым образом проточных ячеек, и оптимизацию перемещения светового пучка относительно флуоресцентных образцов для достижения реализации SIM, которая может использоваться в методах линейного сканирования. Такой результат достигается тем, что проецируют оптический рисунок на биологический образец и захватывают первое изображение оптического рисунка, наложенного на биологический образец; сдвигают по фазе проецируемый оптический рисунок относительно биологического образца и захватывают по меньшей мере второе изображение смещенного по фазе оптического рисунка, наложенного на биологический образец; и реконструируют изображение высокого разрешения, представляющее биологический образец, на основе первого захваченного изображения и по меньшей ...

Система и способ транспортировки предметов

СИСТЕМЫ И СПОСОБЫ БИОХИМИЧЕСКОГО АНАЛИЗА, ВКЛЮЧАЮЩИЕ ОСНОВНОЙ ПРИБОР И СЪЕМНЫЙ КАРТРИДЖ

СИСТЕМЫ И СПОСОБЫ БИОХИМИЧЕСКОГО АНАЛИЗА, ВКЛЮЧАЮЩИЕ ОСНОВНОЙ ПРИБОР И СЪЕМНЫЙ КАРТРИДЖ

КАРТРИДЖ ДЛЯ ОЧИСТКИ НУКЛЕИНОВОЙ КИСЛОТЫ

УСТРОЙСТВО И СПОСОБ РАЗДЕЛЕНИЯ И АНАЛИЗА КРОВИ

... 1. Устройство, включающее по меньшей мере разделительные средства для разделения плазмы крови и красных кровяных телец, где разделительные средства включают сжимающее приспособление для продавливания по меньшей мере части крови через фильтр, в котором предусмотрены по меньшей мере первый сборный отсек для сбора отделенной кровяной плазмы и по меньшей мере один реагент, который размещен в первом сборном отсеке или может быть введен в него для реакции с субстанциями или организмами, присутствующими в плазме крови. ! 2. Устройство по п.1, в котором разделительные средства включают камеру для введения крови, где камера содержит текучую среду в качестве разбавителя для крови, в котором размещен фильтр, через который может проходить кровяная плазма из крови, разбавленная указанной текучей средой в качестве разбавителя, но не могут проходить по меньшей мере красные кровяные тельца. ! 3. Устройство по п.1, в котором по меньшей мере один реагент размещен в части стенки первого сборного отсека или ...

Optics collection and detection system and method

Optics collection and detection systems are provided for measuring optical signals from an array of optical sources over time. Methods of using the optics collection and detection systems are also described.

Thermal validation apparatus, assembly including a device for the thermal processing of biological samples and such an apparatus, and method for manufacturing such an apparatus

The invention relates to a thermal validation apparatus including at least one lining, each lining defining a sink and intended to be inserted into a respective recess of the thermal processing device for heating or cooling biological samples, as well as a respective temperature probe arranged in each sink. Each sleeve is made of a plastic material.

Body fluid analysis fixture

The present invention is intended to, in a configuration in which a liquid container for analysis sealed by a sealing member is penetrated by a penetrating member, prevent the sealing member from being unexpectedly broken to leak a liquid for analysis. Also, the present invention has: a container holder part that holds a liquid container for analysis; a penetrating member that penetrates a sealing member of the liquid container for analysis; a first moving mechanism that enables the penetrating member to be moved between a hole making position and a receding position; and a second moving mechanism that enables the penetrating member moved to the hole making position by the first moving mechanism to be moved toward the sealing member to a penetrating position where the penetrating member penetrates the sealing member.

Electromagnetic multiplex assay biosensor

A method is provided for determining the presence of multiple different target analytes in a liquid sample using electrophoretic separation and magnetic labels within a self-contained reaction cartridge and an external magnetic sensor for detection. Magnetic labels are bound to target analytes through specific binding elements. By electrophoretic separation, the multiple different targets can be sorted according to their specific sizes and inherent molecular charges for better detection resolution and specificity. After the separation process, the target analytes are then recognized and trapped by the detection binding elements within the reaction cartridge. A magnetic field generator provides a changeable magnetic field that causes the bounded magnetic labels and target analytes to produce a resonance disruption detectable by a magnetic sensor. The sensor can provide a digital binary value to indicate whether or not a label particle is bound and that determines the presence of target analytes.

Rapid Test Apparatus

Provided herein are methods and devices for rapid testing of solid, semi-solid, or liquid specimens, such a stool, blood, urine, saliva, or swab specimens of the cervix, urethra, nostril, throat, and for environmental testing.

Device for a test strip holder, method and arrangement

For the simplified use of a test strip holder, a device having a holding or positioning device is provided, comprising a mixing chamber, a lid for closing the mixing chamber, and an obvious fluid opening for a fluid passage from the mixing chamber to the test strip holder. This allows for the test strip holder to be inserted into the device in a simple way and a sample can be prepared in the mixing chamber of the device. The device is provided with a mixing chamber that can be closed, wherein a reaction partner, for example a gold conjugate, can be dissolved in the sample for increasing the sensitivity of the test strip.

Integrated modular unit including an analyte concentrator-microreactor device connected to a cartridge-cassette

The present invention relates to an immunoaffinity device for capturing one or more analytes present at high or low concentrations in simple or complex matrices. The device is designed as an integrated modular unit and connected to capillary electrophoresis or liquid chromatography for the isolation, enrichment, separation and identification of polymeric macromolecules, primarily protein biomarkers. The integrated modular unit includes an analyte-concentrator-microreaction device connected to a modified cartridge-cassette.

Integrated plasmonic nanocavity sensing device

An integrated plasmonic sensing device is described wherein the integrated device comprises: at least one optical source comprising a first conductive layer and a second conductive layer, and a optical active layer between at least part of said first and second conductive layers; at least one nanocavity extending through said first and second conductive layers and said optical active layer, wherein said optical source is configured to generate surface plasmon modes suitable for optically activating one or more resonances in said nanocavity; and, at least one optical detector comprising at least one detection region formed in said substrate in the vicinity of said nanocavity resonator, wherein said optical detector is configured to sense optically activated resonances in said nanocavity.

A microfluidic cell and a spin resonance device for use therewith

A microfluidic cell comprising: a microfluidic channel ( 32 ) for receiving a fluid sample; and a sensor ( 30 ) located adjacent the microfluidic channel; wherein the sensor comprises a diamond material comprising one or more quantum spin defects ( 34 ). In use, a fluid sample is loaded into the microfluidic cell and the fluid is analysed via magnetic resonance using the quantum spin defects.

MICROFLUIDIC SYSTEMS FOR EPIDERMAL SAMPLING AND SENSING

A microfluidic system includes a flexible substrate having a skin-facing surface and a back-facing surface; a microfluidic network at least partially embedded in or supported by the flexible substrate; a sensor fluidically connected to the microfluidic network, wherein the microfluidic network is configured to transport a biofluid from a skin surface to the sensor; and a capping layer, having a capping layer skin-facing surface and a back-facing surface, wherein the back-facing surface of the capping layer is attached to the skin-facing surface of the substrate. The flexible substrate is at least partially formed of a thermoplastic elastomer or a polymer configured to provide a high barrier to vapor or liquid water transmission. 1. A microfluidic system , comprising:a flexible substrate having a skin-facing surface and a back-facing surface;a microfluidic network at least partially embedded in or supported by the flexible substrate;a sensor fluidically connected to the microfluidic network, wherein the microfluidic network is configured to transport a biofluid from a skin surface to the sensor; anda capping layer, having a capping layer skin-facing surface and a back-facing surface, wherein the back-facing surface of the capping layer is attached to the skin-facing surface of the substrate;wherein the flexible substrate is at least partially formed of a thermoplastic elastomer or a polymer configured to provide a high barrier to vapor or liquid water transmission.2. The microfluidic system of claim 1 , wherein the flexible substrate and the capping layer have a common additive.3. The microfluidic system of claim 1 , wherein the capping layer is at least partially formed of a thermoplastic elastomer and an additive.4. The microfluidic system of claim 3 , wherein each of the flexible substrate and the capping layer is formed of a common thermoplastic elastomer composition claim 3 , or a different thermoplastic elastomer composition.5. The microfluidic system of claim ...

Smart Storage Container for Health Logistics

Provided herein are methods and related devices for preprocessing a biological sample during transit. The method may comprise the steps of: storing a biological sample in a storage container having walls that defines a storage volume; transporting the storage container with the stored biological sample to a sample processing facility; controlling one or more storage container parameters during the transporting step to initiate preprocessing of the biological sample; wherein the controlling step improves a processing parameter at the sample processing facility. 2. The method of claim 1 , wherein the improved biological sample processing parameter is one or more of:a decrease in a number of processing steps at the sample processing facility;a decrease in a processing time required at the sample processing facility upon delivery of the biological sample to the sample processing facility; oran improved integrity of the sample processing outcome.3. The method of claim 1 , further comprising the step of sensing a storage container parameter during the transporting step with one or more sensors.4. The method of claim 1 , further comprising the step of collecting the biological sample claim 1 , wherein the biological sample comprises a body fluid sample claim 1 , a tissue sample claim 1 , or an environmental sample.5. The method of claim 1 , wherein the biological sample is used in an assay selected from the group consisting of: a radiological exposure assay; a cancer assay; a chemical assay; a biothreat exposure assay; a diagnostic assay; a molecular imaging assay; and a spectroscopic assay.6. The method of claim 1 , wherein the one or more controlled storage container parameters is one or more of: temperature claim 1 , reagent introduction claim 1 , fixative introduction claim 1 , centrifugation claim 1 , mixing claim 1 , washing claim 1 , isolation claim 1 , separation of one or more sample constituents claim 1 , liquid manipulation claim 1 , gas manipulation claim 1 , ...

SYSTEM AND METHOD FOR DETECTION AND SORTING OF CELLS

A system and method for detection of cells and sorting of cells are disclosed. Target cells, such as circulating tumor cells (CTCs) or antigen-specific antibody producing circulating memory B cells from COVID-19 patients, may be of interest. Magnetic beads may be bound to the target cells. After which, the bead-bound target cells may be identified using an applied magnetic field. In one example, magnetic sensors may be used to detect movement of the bead-bound target cells responsive to an applied magnetic field. In another example, an optical sensor may be used to detect movement of the bead-bound target cells responsive to an applied magnetic field. Further, separate from identification of the target cells, the bead-bound target cells may be sorted using an applied magnetic field. In this way, a magnetic field may be used for target cell identification and target cell sorting in order to detect and collect target cells of interest at the single-cell resolution. 1. An apparatus configured to determining whether a magnetic bead-labeled target cell is present in a fluid , the apparatus comprising:a well configured to house the fluid containing particles and including at least one outlet;at least one magnetic field generator configured to generate a magnetic field to at least a part of the well;one or more sensors configured to generate sensor data; and control the magnetic field generator to generate the magnetic field to the at least a part of the well;', 'identify, based on the sensor data responsive to the magnetic field, the magnetic bead-labeled target cell and an associated location within the well; and', 'control the magnetic field generator, based on the associated location within the well of the magnetic bead-labeled target cell and the at least one outlet, in order to move the magnetic bead-labeled target cell toward the at least one outlet, thereby sorting the magnetic bead-labeled target cell, in order to remove the magnetic bead-labeled target cell from ...

DETECTION MODULE

We describe a detection module useful with an apparatus and/or system for conducting luminescence assays, and a kit, a system, an apparatus, and a method incorporating the detection module. 13-. (canceled)4. A detection module configured for placement and functional engagement with an apparatus for conducting luminescence assays , said detection module being a replaceable unit including a detection module housing , and wherein the following components are disposed within said housing:(a) a light detector; and(b) firmware configured to interface with software running on the apparatus.5. The detection module according to claim 4 , comprising an engagement mechanism for said placement and functional engagement with the apparatus.6. The detection module according to claim 5 , wherein said engagement mechanism comprises a vertical tab and the apparatus comprises an engagement pin claim 5 , wherein alignment and engagement of said vertical tab and said engagement pin causes said detection module to align and engage said detection module with said apparatus.7. The detection module according to claim 5 , wherein said engagement mechanism comprises a locking mechanism configured to align with and engage said apparatus.8. The detection module according to claim 4 , further comprises an imaging system and said light detector is a component of said imaging system and said imaging system is configured to align the housing in X claim 4 , Y claim 4 , and/or Z-direction relative to a target positioned within said apparatus.9. The detection module according to claim 8 , wherein said target is a plate carriage claim 8 , a plate carriage component claim 8 , a multi-well plate claim 8 , a well within a multi-well plate claim 8 , a sector of said multi-well plate comprising two or more wells claim 8 , and combinations thereof.10. The detection module according to claim 8 , wherein said apparatus further comprises a light-tight enclosure and said imaging system is configured to align ...

MICROFABRICATED DEVICE WITH MICRO-ENVIRONMENT SENSORS FOR ASSAYING COAGULATION IN FLUID SAMPLES

The present invention relates to sample analysis cartridges comprising micro-environment sensors and methods for assaying coagulation in a fluid sample applied to the micro-environment sensors, and in particular, to performing coagulation assays using micro-environment sensors in a point of care sample analysis cartridge. For example, the present invention may be directed to a sample analysis cartridge including an inlet chamber configured to receive a biological sample, and a conduit fluidically connected to the inlet chamber and configured to receive the biological sample from the inlet chamber. The conduit may include a micro-environment prothrombin time (PT) sensor, and a micro-environment activated partial thromboplastin time (aPTT) sensor. 1. An integrated circuit chip comprising:a micro-environment prothrombin time (PT) sensor;a micro-environment activated partial thromboplastin time (aPTT) sensor or an micro-environment activated clotting time (ACT) sensor, wherein the micro-environment PT sensor is on a same vertical plane as the micro-environment aPTT sensor or the micro-environment ACT sensor;a first temporary electrical connector connected via a wiring to the micro-environment PT sensor; anda second temporary electrical connector connected via another wiring to the micro-environment aPTT sensor or the micro-environment ACT sensor.2. The integrated circuit chip of claim 1 , wherein the micro-environment PT sensor comprises at least one reagent for inducing extrinsic coagulation pathway.3. The integrated circuit chip of claim 1 , wherein the micro-environment aPTT sensor or the micro-environment ACT sensor comprises at least one reagent for inducing intrinsic coagulation pathway.4. The integrated circuit chip of claim 1 , wherein the micro-environment PT sensor is an amperometric PT sensor claim 1 , wherein the micro-environment aPTT sensor is an amperometric aPTT sensor claim 1 , wherein the micro-environment ACT sensor is an amperometric ACT sensor claim ...

MULTI FUNCTION SPINNING PLATFORM

A rotatable platform is provided for use in Lab-on-disc (LoD) applications. A LoD microfluidic device has the rotatable platform and is suitable for analysis of a fluid sample. Real-time monitoring of cells is also provided, using a centrifugal microfluidic platform. A mobile LoD device is provided, which can be used in remote destinations and for point of care applications. A method for monitoring microorganisms under the constant supply of nutrients includes the step of inoculating the cells in a culture chamber in a rotatable microfluidic platform, rotating the platform such that liquid in a reservoir connected to or located on the platform, the liquid comprising nutrients for the cells, is constantly supplied to the cells in the culture chamber by means of shear/centrifugal force resulting from rotating the platform, and continuously monitoring the cells during rotation of the platform by means of imaging, electrochemical and/or electrical measurements. 145-. (canceled)46. A portable centrifugal microfluidic device comprising:a supporting base for supporting the device on a surface;a centrifugal microfluidic disc comprising at least one sample chamber, wherein the microfluidic disc is configured for engaging with a rotatable platform or a motor for spinning the microfluidic disc; an imaging device for imaging a sample in the sample chamber(s); and/or', 'an electrochemical analyser configured for performing electrochemical measurements of a sample in the sample chamber(s); and/or', 'a miniaturized Raman system for in-situ Raman mapping of a sample in the sample chamber(s);, 'a monitoring system comprisingwherein the monitoring system is configured for imaging and/or analysing one or more samples in the sample chamber(s) during rotation of the centrifugal microfluidic disc.47. The portable centrifugal microfluidic device according to claim 46 , wherein the electrochemical analyser is electrically connected to electrodes provided in the sample chamber claim 46 , ...

Device and method for determining liquid contact and liquid volume in a liquid dispenser based on sound

Devices and methods for determining whether a contact of a liquid dispenser with a liquid based on sound and/or for determining a liquid volume in the liquid dispenser are provided. According to an embodiment, the liquid dispenser includes a sound generator and an acoustic sensor, and at least one of the sound generator or the acoustic sensor is disposed within the dispense chamber portion. According to an embodiment, the liquid dispenser includes a sound generator and an acoustic sensor, and further includes one or more side conduits, where at least one of the sound generator or the acoustic sensor is disposed within a cavity of a respective one of the one or more side conduits, wherein the cavity and a connector of each of the one or more side conduits are free from resonance within a frequency range of the sound sensed by the acoustic sensor.

HANDHELD SENSOR FOR RAPID, SENSITIVE DETECTION AND QUANTIFICATION OF SARS-CoV-2 FROM SALIVA

Various examples are provided for disposable medical sensors that can be used for detection of SARS-CoV-2 antigen, cardiac troponin I, or other biosensing applications. In one example, a medical sensing system includes single-use disposable test strip comprising a functionalized sensing area configured to detect SARS-CoV-2 antigen and a portable sensing and readout device including pulse generation circuitry that can generate synchronized gate and drain pulses for detection and quantification of SARS-CoV-2 antigen in biological samples. In another example, a method includes providing a saliva sample to a functionalized sensing area configured to detect SARS-CoV-2 antigen, generating synchronized gate and drain pulses for a transistor, the gate pulse provided via electrodes of the functionalized sensing area, and sensing an output of the transistor that is a function of a concentration of SARS-CoV-2 antigen in the sample. 1. A medical sensing system , comprising:a single-use disposable test strip comprising a functionalized sensing area disposed between first and second electrodes, the functionalized sensing area configured to detect SARS-CoV-2 antigen; and pulse generation circuitry configured to generate synchronized gate and drain pulses, the first electrode of the disposable test strip electrically coupled to a gate pulse output of the pulse generation circuitry; and', 'a transistor having a drain electrically controlled by a drain pulse output of the pulse generation circuitry, and a gate electrically coupled to the second electrode of the disposable test strip., 'a portable sensing and readout device comprising2. The medical sensing system of claim 1 , wherein the functionalized sensing area is functionalized with anti-SARS-CoV-2 antibody.3. The medical sensing system of claim 2 , wherein the anti-SARS-CoV-2 antibody is bound to a gold (Au) sensing area surface.4. The medical sensing system of claim 3 , wherein the Au sensing area surface is treated with thio- ...

METHODS, SYSTEMS AND COMPOSITIONS FOR FUNCTIONAL IN VITRO CELLULAR MODELS OF MAMMALIAN SYSTEMS

The present invention comprises methods, systems and compositions comprising cell culture analog systems, comprising components which optionally comprise biologically functional cells, and the components and systems function similarly to in vivo conditions. 137-. (canceled)38. A method of assessing one or more effects of varying an input variable or a cell culture characteristic on a microfluidic cell culture analog system , the method comprising:varying an input variable or a cell culture characteristic to which a microfluidic cell culture analog system is exposed, the microfluidic cell culture analog system comprising one or more organ components, each organ component comprising one or more chambers, and each organ component (i) being microfluidically coupled to one another, and (ii) comprising cells cultured on a surface;recording, over a duration of time, changes in measured electrophysiological properties, contractile properties or both in response to the varying input variable or cell culture characteristic;in which the electrophysiological properties, if measured, are measured from a first population of cells cultured on a surface comprising a microelectrode array housed in a first chamber and the contractile properties, if measured, are measured from a second population of cells cultured on a surface comprising a cantilever array housed in a second chamber;the recorded changes providing an assessment of the one or more effects of varying an input variable or a cell culture characteristic.39. The method of claim 38 , wherein the duration of time is a time period of days.40. The method of claim 38 , wherein the multiple microfluidically coupled organ components include a cardiac component claim 38 , and the method further comprises measuring at least one electrophysiological property from cardiomyocyte cells cultured on a microelectrode array of the cardiac component and simultaneously measuring at least one contractile property from cardiomyocyte cells ...

Synthesis apparatus and method

Apparatus for biochemical synthesis comprises a reaction vessel, a temperature control device for the reaction vessel, a plurality of reservoirs for reaction components, and a supply/withdrawal means, e.g. comprising reciprocating syringe pumps and switchable valves. Agitation of a reaction mixture is effected by withdrawing a part of the mixture from the reaction vessel and returning it thereto.

MULTI-STAGE ORAL-FLUID TESTING DEVICE

In an embodiment, the claimed invention includes an oral-fluid collection and testing device that. is simple to operate. The device includes a body assembly and a cap assembly that are easy to handle by a user. A collection sponge projects from an end of the body assembly for absorbing the oral fluid of a donor, A cap assembly is easily aligned with the body assembly by way of visual alignment indicators on both the body and the cap. Once the cap is aligned with the body, a user simply pushes the cap onto the ‘body, which causes a first stage fluid, flow. More specifically, a buffer fluid is released from the cap and mixes with the oral fluid collected on the sponge—After waiting a short time* the cap is rotated, then pushed again, causing a second-stage fluid flow in which the sponge is compressed such that the buffer fluid/oral fluid exits the sponge and flows toward a. pair of test strips. A user can then easily view the test results by observing a visual indication, such as a color change of the test strips through a viewing window. 19-. (canceled)10. A testing device for collecting and testing a bodily fluid for substances , the testing device comprising: a cap having a first end and a second end opposite the first end, and configured to receive a portion of a body assembly at the second end, the cap defining a first cap cavity at the first end and a second cap cavity at the second end;', 'a fluid container disposed in the first cavity, wherein the fluid container includes a sealing membrane and contains a mixing fluid for mixing with a bodily fluid; and', 'a plunger with an end wall movably disposed in the second cavity, wherein the plunger has a first position and a second position, and wherein at the first position, the end wall of the plunger is configured to not compress a collection sponge attached to the body assembly when received by the cap., 'a cap assembly, including11. The testing device of claim 10 , wherein the plunger is not moved and stable at ...

NUCLEIC ACID EXTRACTION AND PURIFICATION CARTRIDGES

Nucleic acid extraction and purification cartridges and systems are provided. The cartridges can be removable and are configured to allow for the concentration of particles of interest, followed by nucleic acid extraction and purification. The cartridges directly contact samples and provide a partial barrier between samples and the reusable components of the system, thereby reducing the probability of clogging the system's microfluidics and fouling the lines, valves, and pumps of the system. Furthermore, these cartridges are designed to purity nucleic acids by removing the majority of inhibitors for down-stream genetic testing. Embodiments may comprise one, two, or three or more channels. In an exemplary embodiment the nucleic acid extraction and purification cartridge comprises a first channel containing a filter disposed therein; and a second channel containing a nucleic acid binding matrix disposed therein, wherein a first end of the cartridge is configured to directly contact a sample comprising a biological material, and wherein a second end of the cartridge Is configured to connect in a reversible fashion to a flow-through automated Instrument that controls fluid flow through the cartridge. 1. A nucleic acid extraction and purification cartridge comprising:a channel containing a nucleic acid binding matrix disposed therein,wherein a first end of the cartridge is configured to directly contact a sample comprising a biological material, andwherein a second end of the cartridge is configured to connect in a reversible fashion to a flow-through automated instrument that controls fluid flow through the cartridge.2. The nucleic acid extraction and purification cartridge of claim 1 , wherein the channel is functionalized with a ligand that binds to a target in a sample or features/elements that allow for specific target enrichment.3. The nucleic acid extraction and purification cartridge of claim 1 , wherein the channel is configured to receive vibration and/or ...

ANALYSIS DEVICE, CARTRIDGE, ANALYSIS SYSTEM AND METHOD FOR TESTING A SAMPLE

An analysis device, a cartridge, an analysis system and a method for preferably testing a biological sample. The analysis system has a sample sensor for monitoring the sample in a receiving cavity of the cartridge. The sample sensor preferably allows sedimentation of the sample to be measured or detected. 141-. (canceled)42. An analysis device for testing a sample by means of a cartridge , comprising:a holder for a cartridge,a sensor apparatus with a sample sensor for monitoring the sample in a receiving cavity of the cartridge, the sample sensor being adapted to detect a supernatant or sedimentation of the sample,means for at least one of electrically, thermally, fluidically or pneumatically connecting the cartridge to the sensor apparatus, anda pump drive for pumping the sample or supernatant to the sensor apparatus or catcher molecules of the cartridge after the supernatant or sedimentation of the sample has been sensed by the sample sensor.43. The analysis device according to claim 42 , wherein the supernatant is blood plasma or blood serum.44. The analysis device according to claim 42 , wherein the sample sensor is adapted to detect at least one of visible light or infrared radiation.45. The analysis device according to claim 42 , wherein the sample sensor is adapted to monitor the sample by a reflection measurement.46. The analysis device according to claim 42 , wherein the holder is adapted to automatically place at least one of the sample sensor or the cartridge in an operating position upon or after insertion of the cartridge so that the sample in the receiving cavity is able to be monitored by the sample sensor in the operating position.47. The analysis device according to claim 42 , further comprising a control apparatus for automatically at least one of starting or controlling testing of the sample based on signals received from the sample sensor.48. The analysis device according to claim 43 , wherein the holder comprises a receiving unit for at least ...

AUTOMATED ANALYSIS DEVICE

Provided is an automated analysis device with which sufficient reaction process data can be acquired irrespective of the scale of the device, and with which it is possible to ensure freedom of the device configuration. An automated analysis device is provided with: a reaction disk which circumferentially accommodates a plurality of reaction vessels a specimen dispensing mechanism which dispenses a specimen into the reaction vessels a reagent dispensing mechanism which dispenses a reagent into the reaction vessels a measuring unit which measures a reaction process of a mixture of the specimen and the reagent in the reaction vessels and a cleaning mechanism which cleans the reaction vessels after measurement. Further, the automated analysis device includes a controller which controls the drive of the reaction disk such that in one cycle the reaction vessels move by an amount A in the circumferential direction in such a way that N and A are mutually prime, B and C are mutually prime, and the relationship A×B=N×C±1 holds, where N is the total number of reaction vessels accommodated in the reaction disk the reaction disk moves through C (where C>1) rotations+an amount equivalent to one reaction vessel after B (where B>2) cycles, and the number of reaction vessels moved in one cycle is A (where N>A>N/B+1). 1. An automatic analysis device , comprising:a reaction disk which accommodates a plurality of reaction vessels capable of accommodating a dispensed specimen and reagent such that the reaction vessels are spaced apart from each other at predetermined intervals circumferentially;a specimen dispensing mechanism which dispenses a predetermined amount of the specimen into the reaction vessel;a reagent dispensing mechanism which dispenses a predetermined amount of the reagent into the reaction vessel;a measuring unit which measures a reaction liquid during a reaction process and/or after reaction of a mixture of the specimen and the reagent in the reaction vessel;a cleaning ...

DEVICE AND METHOD FOR ANALYZING TOTAL CYANIDE IN WATER SAMPLES

A device and a method of analyzing total cyanide in water samples, including a mixing tube, a stripping tube, a sampling pipe, a reaction pipe, a peristaltic pump, an air pump, a UV digester, a heater, a condenser, a drain pipe and a water sample detector. The mixing tube, the stripping tube, the sampling pipe and the reaction pipe are respectively connected to the peristaltic pump. The mixing tube is connected to the reaction pipe. A bottom of the stripping tube, the air pump, the UV digester, the heater and the condenser and are sequentially connected. The condenser is connected to the mixing tube. An upper portion of the stripping tube, the sampling pipe and the reaction pipe are connected to the drain pipe. An absorbance of the water in the mixing tube is measured by the sample detector, and used to calculate the total cyanide content by spectrophotometry. 1. A device for analyzing total cyanide in a water sample , comprising: a mixing tube , a stripping tube , a sampling pipe , a reaction pipe , a peristaltic pump , an air pump , a UV digester , a heater , a condenser , a drain pipe and a water sample detector;wherein the mixing tube, the stripping tube, the sampling pipe and the reaction pipe are respectively connected to the peristaltic pump; the mixing tube is connected to the reaction pipe; a bottom of the stripping tube, the air pump, the UV digester, the heater and the condenser are sequentially connected; the condenser is connected to the mixing tube; an upper portion of the stripping tube, the sampling pipe and the reaction pipe are respectively connected to the drain pipe; and the water sample detector is able to detect and analyze parameters of the water sample in the mixing tube.2. The device of claim 1 , wherein the reaction pipe and the sampling pipe each comprise a plurality of three-way valves claim 1 , and respective three-way valves comprise a normally closed end claim 1 , a common end and a normally open end.3012345. The device of claim 2 , ...

METHODS OF MEASURING HEMATOCRIT IN FLUIDIC CHANNELS INCLUDING CONDUCTIVITY SENSOR

Methods of determining hematocrit in a whole blood sample utilizing a system, the system including a first portion, the first portion including at least one fluid channel; a fluid actuator; an analysis sensor disposed within the fluid channel; a conductivity sensor including two electrodes located no more than 10 mm away from each other and disposed within the fluid channel; and an introducer; a second portion, the second portion including at least one well, the well containing at least one material, wherein one of the first or second portion is moveable with respect to the other, wherein the introducer is configured to obtain at least a portion of the material from the at least one well and deliver it to the fluid channel, and wherein the fluid actuator is configured to move at least a portion of the material in the fluid channel, the method including measuring the resistance over substantially the entire portion of a whole blood sample; and calculating an average hematocrit level of the whole blood sample based on the measured resistance. 1. A device comprising: at least one fluid channel;', 'a fluid actuator;', 'an analysis sensor disposed within the fluid channel;', 'a conductivity sensor comprising two electrodes that are no more than 10 millimeters away from each other disposed within the fluid channel; and', 'an introducer;', 'at least one well, the well containing at least one material,', 'a second portion, the second portion comprising, 'wherein one of the first or second portion is moveable with respect to the other, wherein the introducer is configured to obtain at least a portion of the material from the at least one well and deliver it to the fluid channel, and wherein the fluid actuator is configured to move at least a portion of the material in the fluid channel., 'a first portion, the first portion comprising2. The device according to claim 1 , wherein the analysis sensor is a resonator sensor.3. The device according to claim 2 , wherein the resonant ...

FLUIDIC CHANNELS INCLUDING CONDUCTIVITY SENSOR AND METHODS OF USE THEREOF

Devices that includes a first portion, the first portion including at least one fluid channel; a fluid actuator; an analysis sensor disposed within the fluid channel; a conductivity sensor disposed within the fluid channel; and an introducer; a second portion, the second portion comprising: at least one well, the well containing at least one material, wherein one of the first or second portion is moveable with respect to the other, wherein the introducer is configured to obtain at least a portion of the material from the at least one well and deliver it to the fluid channel, and wherein the fluid actuator is configured to move at least a portion of the material in the fluid channel. 1. A device comprising: at least one fluid channel;', 'a fluid actuator;', 'an analysis sensor disposed within the fluid channel;, 'a first portion, the first portion comprising an introducer;', 'at least one well, the well containing at least one material,', 'a second portion, the second portion comprising, 'wherein one of the first or second portion is moveable with respect to the other, wherein the introducer is configured to obtain at least a portion of the material from the at least one well and deliver it to the fluid channel, and wherein the fluid actuator is configured to move at least a portion of the material in the fluid channel., 'a conductivity sensor disposed within the fluid channel; and'}2. The device according to claim 1 , wherein the analysis sensor is a resonator sensor.3. The device according to claim 2 , wherein the resonant sensor is a bulk acoustic wave (BAW) sensor.4. The device according to claim 1 , wherein the conductivity sensor is located upstream of the analysis sensor.5. The device according to claim 1 , wherein the conductivity sensor comprises two electrodes.6. The device according to claim 5 , wherein the two electrodes comprise gold.7. A method of using a system claim 5 , the system comprising:a first portion, the first portion comprising:at least one ...

DISSOLUTION DEVICE

A dissolution device for measuring a dissolution rate of a test sample in a fluid, the device comprising a first cavity and a second cavity, wherein each of the first and second cavities has a fluid inlet for connection to a fluid supply, and wherein the device comprises an opening between the first and second cavities, and the device comprises a sample support configured to position the test sample across the opening. 1. A dissolution device for measuring a dissolution rate of a test sample in a fluid , the device comprising:a first cavity; anda second cavity;wherein each of the first and second cavities has a fluid inlet for connection to a fluid supply; andwherein the device comprises an opening between the first and second cavities, and the device comprises a sample support configured to position the test sample across the opening.2. The device of claim 1 , wherein the sample support comprises a permeable support configured to support the test sample across the opening.3. (canceled)4. The device of claim 1 , further comprising first and second fluid supplies claim 1 , wherein the first and second cavities are in fluid communication respectively with the first and second fluid supplies via the respective fluid inlets claim 1 , and wherein the first fluid supply is at a static pressure claim 1 , measurable at a point within the first cavity and adjacent the test sample claim 1 , that is equal to or higher than a static pressure of the second fluid supply claim 1 , measurable at a point within the second cavity and adjacent the test sample.5. The device of claim 4 , wherein at least one of the first and second fluid supplies is configured to be substantially free of pressure pulsation.6. The device of claim 4 , wherein the first and second fluid supplies are configured to produce a substantially constant relative pressure therebetween.7. The device of claim 4 , wherein the static pressure of at least one of the first and second fluid supplies is provided by a ...

CELL SORTING DEVICES

In one example in accordance with the present disclosure, a cell sorting device is described. The cell sorting device includes a microfluidic channel to serially transport individual cells from a volume of cells along a flow path. A sensor disposed in the microfluidic channel distinguishes between a cell to be analyzed and waste fluid. The cell sorting device includes at least two fluid transport devices disposed within the microfluidic channel. The at least two fluid transport devices include a cell ejector to, responsive to detection of a cell to be analyzed, eject the cell to be analyzed from the cell sorting device and a waste transport device to direct the waste fluid to a waste reservoir. 1. A cell sorting device , comprising:a microfluidic channel to serially transport individual cells from a volume of cells along a flow path;a sensor disposed in the microfluidic channel to distinguish between a cell to be analyzed and waste fluid; and an ejector to, responsive to detection of a cell to be analyzed, eject the cell to be analyzed from the cell sorting device; and', 'a waste transport device to direct the waste fluid to a waste reservoir., 'at least two fluid transport devices disposed within the microfluidic channel, the at least two fluid transport devices comprising2. The cell sorting device of claim 1 , wherein the waste transport device comprises at least one waste ejector to eject claim 1 , through a waste orifice claim 1 , the waste fluid from the cell sorting device.3. The cell sorting device of claim 2 , wherein the waste ejector operates independently of the sensor.4. The cell sorting device of claim 2 , wherein responsive to detection of a cell to be analyzed claim 2 , the waste ejector is deactivated.5. The cell sorting device of claim 2 , wherein the at least one waste ejector comprises multiple waste ejectors.6. The cell sorting device of claim 1 , wherein the waste transport device is an integrated pump disposed in the microfluidic channel to ...

Wafer level sequencing flow cell fabrication

A method for forming sequencing flow cells can include providing a semiconductor wafer covered with a dielectric layer, and forming a patterned layer on the dielectric layer. The patterned layer has a differential surface that includes alternating first surface regions and second surface regions. The method can also include attaching a cover wafer to the semiconductor wafer to form a composite wafer structure including a plurality of flow cells. The composite wafer structure can then be singulated to form a plurality of dies. Each die forms a sequencing flow cell. The sequencing flow cell can include a flow channel between a portion of the patterned layer and a portion of the cover wafer, an inlet, and an outlet. Further, the method can include functionalizing the sequencing flow cell to create differential surfaces.

SAMPLING DEVICE FOR SAMPLING FROM A SURFACE OF AN INDUSTRIAL VESSEL WITHOUT REQUIRING A PERSON TO DESCEND INTO AN INDUSTRIAL VESSEL, USING A SWAB

Disclosed is a module for sampling from a surface of an industrial vessel without requiring a person to descend into the industrial vessel, using a swab including a stem and a sampling tip, including a swab holder and a conveyor; the swab holder is adapted to receive and secure one end of the swab stem; and the conveyor is configured to set the swab holder in motion on the surface of the vessel. 1. A module for sampling from a surface of an industrial vessel without requiring a person to descend into the industrial vessel using a swab comprising a stem and a sampling tip , comprising a swab holder and a conveyor;the swab holder being adapted to receive and secure one end of the stem of the swab; andthe conveyor being configured to move the swab holder at the surface of the vessel.2. The module according to claim 1 , wherein the conveyor is configured to move the swab holder parallel to the surface.3. The module according to claim 2 , wherein the conveyor is configured to move the swab holder in translational motion along one or two axes parallel to the surface.4. The module according to claim 3 , wherein the conveyor comprises a motor and a transmission assembly for moving the swab holder along a first axis of travel parallel to the surface;the transmission assembly comprising a first annular belt, a second annular belt, a set of four pinions disposed so as to form the corners of a rectangle, and a conveyor bar to which the swab holder is secured and a first end of which is secured to the first annular belt and a second end is secured to the second annular belt so that the conveyor bar is parallel to two opposite sides of the rectangle formed by the set of four pinions;wherein the first belt is disposed so as to surround two pinions on one of the sides of the rectangular perpendicular to the conveyor bar thereby forming two parallel lines, the first end of the conveyor bar being secured to the line the innermost within the rectangle; andwherein the second belt is ...

SCREENING DEVICE FOR ANALYSIS OF BODILY FLUIDS

A screening device for testing saliva for the presence of certain constituents. The device has a D-shaped profile when viewed from above and includes a first wall with planar front face through which all of the test strips are viewed and a curved rear second wall. The device also includes a floor surface with an uppermost portion extending from the rear wall to a lowermost portion at the first wall such that saliva squeezed from a sampler at a location between the upper most portion and the lowermost portion flows downwardly to the test strips. 135-. (canceled)36. A device for testing saliva , the device comprising:a cup comprising an outer planar forward wall connecting to an outer curved rearward wall providing a D-shaped outer perimeter of the device when viewed from below, the outer planar forward wall providing the straight portion of the “D” and the outer curved rearward wall providing the remainder of the “D”, the cup having a lower edge that registers the cup in an upright position, an inclined plate traversing continuously upwardly and rearwardly between the planar forward wall and the curved rearward wall, the inclined plate having a lowermost portion of the plate proximate the planar front wall and an uppermost portion of the inclined plate exclusively at the rearward wall and the inclined plate defining a cavity with a single lowermost extremity of the cavity positioned at the planar forward wall;a test strip holder positioned in the cavity adjacent the planar forward wall of said cup, a plurality of test strips positioned between the test strip holder and the forward wall, the plurality of test strips extending to the single lowermost extremity of the cavity and the plurality of test strips comprising all of the test strips of the device;a cap portion positioned at a top portion of the cup, the cap portion having a D-shaped outer perimeter when viewed from above, the D-shaped outer perimeter providing an outermost periphery of the device when viewed ...

Sieve Valves, Microfluidic Circuits, Microfluidic Devices, Kits, and Methods for Isolating an Analyte

The invention generally provides a sieve valve including: a substrate defining a channel; a flexible membrane adapted and configured for deployment at an intersection with the channel; and one or more protrusions extending into the channel from the substrate or the flexible membrane. The one or more protrusions define a plurality of recesses extending beyond the intersection between the channel and the flexible membrane; 1. A method of isolating an analyte , the method comprising:loading a sample into a holding chamber of a microfluidic circuit, the microfluidic circuit including one or more sieve valves, wherein the holding chamber is in fluid communication with an input port of a mixing circuit,capturing an analyte on a capture substrate in the mixing circuit;washing the capture substrate to remove uncaptured components; and a substrate defining a channel;', 'a flexible membrane adapted and configured for deployment at an intersection with the channel; and', 'one or more protrusions extending into the channel from the substrate or the flexible membrane, the one or more protrusions defining a plurality of recesses extending beyond the intersection between the channel and the flexible membrane; and, 'releasing the analyte from the capture substrate, wherein the sieve valve comprises'}passing the sample into the mixing circuit.2. The method of claim 1 , wherein the capture substrate is loaded into the microfluidic circuit claim 1 , the holding chamber claim 1 , and/or the mixing circuit.3. The method of claim 1 , wherein the capture substrate comprises a bead claim 1 , microbead claim 1 , surface of the microfluidic circuit claim 1 , or a capture reagent.4. The method of claim 1 , wherein the mixing circuit comprises a plurality of chambers in fluid communication with one or more holding chambers claim 1 , wherein the one or more holding chambers and/or the mixing circuit comprises the capture substrate for isolating the analyte.5. The method of claim 4 , wherein the ...

Sensor apparatus

A sensor apparatus according to an embodiment of the present invention includes an element substrate, an element electrode located on an upper surface of the element substrate, an insulating member covering at least a part of the element electrode, and a detection part that includes an immobilizing film located on the upper surface of the element substrate or an upper surface of the insulating member, and performs a detection of a detection object contained in a specimen. A surface roughness of the immobilizing film is smaller than a surface roughness of the element electrode. In a sensor apparatus of other embodiment of the present invention, an amount of oxygen in a surface layer part of the immobilizing film is smaller than an amount of oxygen in a surface layer part of the element electrode.

TARGET SUBSTANCE DETECTION DEVICE AND TARGET SUBSTANCE DETECTION METHOD

To detect a target substance accurately and effectively, a target substance detection device includes: a liquid-sample introducing plate formed from a light-transmissive plate having a surface on which a liquid sample including a target substance and magnetic particles forming a conjugate with the target substance is introduced, and enabling propagating transmitted light of light irradiated from the rear face upward of the surface as propagated light; a rear face light irradiation unit configured to be able to irradiate the liquid-sample introducing plate with light from the rear face; a first magnetic field application unit disposed on the side of the surface of the liquid-sample introducing plate , and configured to apply a magnetic field to move the conjugate in the liquid sample that is introduced on the surface of the liquid-sample introducing plate in the direction away from the liquid-sample introducing plate ; and an optical-signal detection unit disposed on the side of the surface of the liquid-sample introducing plate , and enabling detection of a change in optical signal based on the propagated light between before and after application of the magnetic field by the first magnetic field application unit 1. A target substance detection device comprising:a liquid-sample holding unit including a liquid-sample introducing plate introducing any one of: a light-transmissive plate having a surface on which a liquid sample including a target substance and magnetic particles forming a conjugate with the target substance is introduced, and capable of propagating transmitted light of light irradiated from a rear face or the surface to the face on an opposite of the irradiated face as propagated light; a reflective plate having a surface on which the liquid sample is introduced and capable of propagating reflected light of light irradiated from the surface upward of the surface as propagated light; and an introducing plate having a surface on which the liquid sample ...

In-line testing development diagnostic analysis

In-line testing and product delivery assemblies, methods, operations, and systems are shown and described. In one embodiment, an in-line testing and product delivery system includes a supply of product having at least one outlet with a valve closure and a downstream delivery line, a recirculation closed loop in fluid communication with the outlet and supply, and a reader to generate a rapid test result from a single use assay for detection of a presence or an absence of an analyte in the supply. The result provides monitoring of a detection of the analyte to block release of product supply into the delivery line.

Systems and methods for a thermal cycler heated cover

A thermal cycler system for use with a sample holder configured to receive a plurality of samples includes a sample block configured to receive the sample holder, a cover lid configured to move in a direction toward the sample block from an open position to a closed position, a heated cover operatively coupled to the cover lid and configured to move in a direction toward the sample block from a raised position to a first lowered position, in which the heated cover contacts the sample holder when the sample holder is received by the sample block, and a drive assembly including a motion guide configured to move in a direction toward the sample block from a first position, wherein the cover lid is in the open position and the heated cover is in the raised position, to a second position, wherein the cover lid is in the closed position.

FLUID DEVICE

A fluid device includes a base material including a flow path through which a solution flows and a first facing surface, a treatment substrate including a second facing surface which faces the first facing surface and in which a treatment unit which comes in contact with the solution and treats the solution is provided, and a sealing portion sandwiched between the first facing surface and the second facing surface, in which a treatment space surrounding the treatment unit with the sealing portion when viewed from a plate thickness direction and connected to the flow path is provided between the treatment substrate and the base material. 1. A fluid device comprising:a base material including a flow path through which a solution flows and a first facing surface;a treatment substrate including a second facing surface which faces the first facing surface and in which a treatment unit which comes in contact with the solution and treats the solution is provided; anda sealing portion sandwiched between the first facing surface and the second facing surface, whereina treatment space surrounding the treatment unit with the sealing portion when viewed from a plate thickness direction and connected to the flow path is provided between the treatment substrate and the base material.2. The fluid device according to claim 1 , wherein:the base material includes a first substrate and a second substrate which are stacked in a plate thickness direction;the flow path is provided between the first substrate and the second substrate;the second substrate includes the first facing surface;a recess constituting the treatment space therein is provided on the first facing surface; anda bottom surface of the recess is arranged facing the treatment unit.3. The fluid device according to claim 2 , wherein:a stepped surface is provided on an inner peripheral surface of the recess to face the second facing surface; andthe sealing portion is sandwiched between the stepped surface and the second ...

ASSAY APPARATUSES, METHODS, AND REAGENTS

Apparatuses, systems, method, reagents, and kits for conducting assays as well as process for their preparation are described. The apparatuses, systems, method, reagents, and kits may be employed in conducting automated analysis in a multi-well plate assay format. 1. An instrument comprising:a light detection system comprising a CCD sensor and an optical lens system, wherein the light detection system is positioned above a single well at a time in a multi-well plate to conduct an electrochemiluminescence analysis of the single well, wherein the area of the CCD sensor is approximately 1× to 2× of an area of the single well,wherein the light detection system further comprises a cooling device sized and dimensioned to cool the CCD sensor,wherein the instrument further comprises a heat removal system comprising at least one fan oriented at an angle to pull heated air exhausted from the cooling device into a flow plenum and out of the instrument.2. The instrument of claim 1 , wherein the optical lens system comprises a plurality of lenses and the plurality of lenses have both spherical and aspherical surfaces.3. The instrument of claim 2 , wherein the area of the plurality of lenses is larger than the area of the single well.4. The instrument of claim 1 , wherein the light detection system is mounted in a substantially vertical direction onto a housing top.5. The instrument of claim 1 , wherein the heat removal system is mounted onto a housing top.6. The instrument of claim 1 , wherein the at least one fan is housed within the flow plenum.7. The instrument of claim 1 , wherein the plenum houses at least one printed circuit board (PCB) and comprises at least one opening to allow electrical connections between the at least one PCB and electrical components outside of the flow plenum.8. The instrument of claim 7 , wherein a flow baffle is positioned within the plenum to minimize recirculation of air within the instrument.9. The instrument of claim 7 , wherein the heated air ...

Automatic human urine detection system

An automatic human urine detection system comprises a control module, a urine collection module, a detection module, an output module and a cleaning module. The control module controls operation of the system and comprises an instruction input unit. The urine collecting module is used for collecting urine to be detected; the detection module comprises a detection probe holder which is used for detecting the urine to be collected and obtains a corresponding detection report according to a detection result; the output module is used for outputting the detection report; and the cleaning module is used for cleaning system components through which the urine flows or is stored in the system. The automatic human urine detection system integrates automatic collection and subsequent automatic detection of human urine, the whole urinalysis process is effectively simplified, the operation process is humanized, and the user experience is good.

MOLECULAR DIAGNOSTIC ASSAY SYSTEM

Improved sub-assemblies and methods of control for use in a diagnostic assay system adapted to receive an assay cartridge are provided herein. Such sub-assemblies include: a brushless DC motor, a door opening/closing mechanism and cartridge loading mechanism, a syringe and valve drive mechanism assembly, a sonication horn, a thermal control device and optical detection/excitation device. Such systems can further include a communications unit configured to wirelessly communicate with a mobile device of a user so as to receive a user input relating to functionality of the system with respect to an assay cartridge received therein and relaying a diagnostic result relating to the assay cartridge to the mobile device. 1. A diagnostic assay system adapted to receive an assay cartridge , the system comprising any one or combination of:a brushless DC (BLDC) motor operatively coupled with any of a door closing and cartridge loading mechanism, a syringe drive, and a valve drive;a door opening/closing mechanism cooperatively coupled with a cartridge loading mechanism and driven by a backdriveable transmission mechanism;a syringe drive operatively coupled with the BLDC motor and controlled based on monitored current draw of the BLDC motor;a valve drive mechanism operatively coupled with a n-phase BLDC motor based on a voltage signal provided by n voltage sensors of the BLDC motor without use of an encoder or position sensors;a sonication horn engageable with the assay cartridge for lysing of biological material within the assay cartridge and operatively coupled with a controller configured to control sonication based on a frequency providing a highest output amplitude as a resonant frequency;a thermal control device having a first thermoelectric cooler thermally engageable with a reaction vessel of the assay cartridge and at least one other thermal manipulation device thermally coupled with the first thermoelectric cooler and controlled so as to increase efficiency of the first ...

Humidified sample preparation station for serial crystallography

Humidified sample preparation station for serial crystallography according to one embodiment is a humidified enclosure that delivers relative humidities above 95% and preferably above 97% in standard operation, and that can allow microscope observation of samples within. Humidified sample preparation station for serial crystallography can be used for preparation of protein crystal samples for examination using X-rays and for protein structure determination by X-ray crystallography, involving addition of liquid to the sample and removal of liquid from the sample using vacuum or suction.

Diagnostic Systems and Methods for Hemolytic Anemias and Other Conditions

An imaging system for imaging a fluid sample includes a light source configured to generate a beam of light, an angled element disposed along an optical path of the beam of light, and a sample cartridge holder configured to receive a sample cartridge and configured to hold the sample cartridge in a first position in which an imaging region of the sample cartridge is disposed along the optical path. The system further includes a sensor configured to capture the beam of light after it passes through the angled element and the imaging region of the sample cartridge. The imaging region of the sample cartridge is configured to receive the sample fluid. A sample cartridge having a cover plate and a fluidics layer is also disclosed. The fluidics layer includes an opening, a fluid channel, and an imaging region configured to receive a whole blood sample. 1. An imaging system for imaging a fluid sample , the system comprising:a light source configured to generate a beam of light;an angled element disposed along an optical path of the beam of light;a sample cartridge holder configured to receive a sample cartridge and configured to hold the sample cartridge in a first position in which an imaging region of the sample cartridge is disposed along the optical path; anda sensor configured to capture the beam of light after the beam of light passes through the angled element and the imaging region of the sample cartridge,wherein the imaging region of the sample cartridge is configured to receive the sample fluid.2. The system of claim 1 , wherein the angled element is disposed between the light source and the sample cartridge.3. The system of claim 1 , further comprising a reflector claim 1 , wherein the sample cartridge is disposed between the light source and the reflector.4. The system of claim 1 , wherein the sample cartridge is disposed between the light source and the sensor.5. The system of claim 1 , wherein the sample cartridge holder is movable relative to the light ...

TEMPERATURE PROFILE ENCODING FOR DIAGNOSTIC TESTS

Provided herein, in some embodiments, are rapid diagnostic tests to detect one or more target nucleic acid sequences (e.g., a nucleic acid sequence of one or more pathogens). In some embodiments, the pathogens are viral, bacterial, fungal, parasitic, or protozoan pathogens, such as SARS-CoV-2 or an influenza virus. In one embodiment, a diagnostic system is provided comprising a control device configured to control one or more parameters and/or actions of a diagnostic test, and a test kit component comprising a physical encoding of control information for the control device. In one embodiment, the control device is configured to receive the control information of the physical encoding and perform one or more actions based at least in part on the control information. In one embodiment, the control device can control one or more temperatures at which a biological sample is to be processed as part of the diagnostic test. 1. A test kit system comprising:a test kit configured to rapidly detect presence of a target nucleic acid in a human sample, including a test kit component; anda control device configured to control at least one parameter of the test kit component.2. The system of claim 1 , wherein the control device comprises a temperature control device configured to control one or more temperatures at which a biological sample is to be processed as part of the test kit to rapidly detect presence of the target nucleic acid.3. The system of claim 2 , wherein the control device controls multiple temperatures at which multiple biological samples are to be processed.4. The system of claim 1 , wherein the test kit component comprises a consumable comprising a physical encoding of control information for the control device claim 1 , wherein the control device is configured to:receive the control information of the physical encoding; andperform one or more actions based at least in part on the control information.5. The system of claim 4 , wherein the control information ...

SYSTEMS AND METHODS FOR A THERMAL CYCLER HEATED COVER

A thermal cycler system for use with a sample holder configured to receive a plurality of samples includes a sample block configured to receive the sample holder, a cover lid configured to move in a direction toward the sample block from an open position to a closed position, a heated cover operatively coupled to the cover lid and configured to move in a direction toward the sample block from a raised position to a first lowered position, in which the heated cover contacts the sample holder when the sample holder is received by the sample block, and a drive assembly including a motion guide configured to move in a direction toward the sample block from a first position, wherein the cover lid is in the open position and the heated cover is in the raised position, to a second position, wherein the cover lid is in the closed position. 1. A thermal cycler system for use with a sample holder configured to receive a plurality of samples , the system comprising:a sample block configured to receive the sample holder;a heated cover configured to move in a direction toward the sample block from a raised position to a first lowered position when the sample holder is received by the sample block, and from the first lowered position to a second lowered position when the sample holder is removed from the sample block; anda first sensor configured to detect whether the heated cover is in the first lowered position,wherein, in the first lowered position, the heated cover is in contact with the sample holder in the first lowered position, andwherein, in the second lowered position, the heated cover is in a position intermediate of the first lowered position and the sample block.2. The thermal cycler system of claim 1 , wherein claim 1 , when the sample holder is received by the sample block and the heated cover is in the first lowered position claim 1 , the heated cover is configured to apply a force to the sample holder.3. The thermal cycler system of claim 2 , wherein the heated ...

MICROFLUIDIC FLOW CELL COMPRISING AN INTEGRATED ELECTRODE, AND METHOD FOR MANUFACTURING SAME

A microfluidic flow cell including an electrode or sensor device which is located inside the flow cell and from which at least one connecting conductor leads to an externally accessible terminal contact. The electrode or sensor device is arranged on an insulated substrate member. The connecting conductor is embedded in the substrate member. The substrate member can be inserted into an opening in the flow cell such that the electrode or sensor device is placed in the flow cell. 115-. (canceled)16. A microfluidic flow cell , comprising: an insulating carrier body; an electrode or sensor device arranged inside the flow cell; and at least one connecting conductor arranged to lead from the electrode or sensor device to an externally accessible terminal contact , wherein the electrode or sensor device is arranged on the carrier body , the connecting conductor is embedded in the carrier body , and the carrier body is insertable into an opening in the flow cell with arrangement of the electrode or sensor device in the flow cell.17. The flow cell according to claim 16 , further comprising a fluid in a hollow space within the flow cell claim 16 , wherein the electrode or sensor device contacts the fluid claim 16 , the opening forming a passage to the hollow space claim 16 , the carrier body being insertable into the passage in a fluid-tight manner claim 16 , and the connecting conductor being embedded in a fluid-tight manner in the carrier body.18. The flow cell according to claim 17 , wherein the carrier body is formed as a plug having an end face and a rotationally symmetrical sealing face.191. The flow cell according to claim $ claim 17 , wherein the sealing face is conical.20. The flow cell according to claim 18 , wherein the sealing face forms a press fit with the passage.21. The flow cell according to claim 16 , wherein the electrode or sensor device is arranged on an end face of the carrier body.22. The flow cell according to claim 16 , wherein the terminal contact is ...

HAND-ROTATABLE PLUG VALVE WITH MOTORIZED LONGITUDINALLY-MOVABLE GATE ROTATING FOR USE AS A STOPCOCK WITH LABORATORY GLASSWARE IN CHEMICAL LABORATORY AUTOMATION AND OTHER APPLICATIONS

A hand-rotatable motorized-aperture plug arrangement for use as a stopcock in articles of traditional and legacy laboratory glassware of arbitrary complexity. The hand-rotatable plug additionally comprises a longitudinally-movable gate whose gate position is changed by a stepper or D.C. electrical motor. As the gate position is changed, the amount of flow can be varied. Hand-rotation operation of the plug itself can be used to force flow turn-off. The gate position can be monitored by a sensor and can be computer-controlled for use with an article of laboratory glassware for controlling and/or directing the flow of chemical materials. 1. A motorized laboratory hand-rotatable stopcock plug for use within an article of chemical glassware comprising at least one stopcock valve for controlling the flow of chemical materials , the motorized laboratory hand-rotatable stopcock plug comprising:a hand-rotatable stopcock plug having an inlet port and an outlet port;an electrically-operated motor associated with the hand-rotatable stopcock plug for insertion installation and hand-rotation operation within a stopcock body of an article of chemical glassware, the hand-rotatable rotatable plug further comprising a longitudinally-movable gate, the longitudinally-movable gate movable to a position determined by the electrically-operated motor,wherein a cross-sectional area of the longitudinally-movable gate varies with respect to the inlet port and the outlet port when the rotatable plug is rotated, andwherein the longitudinally-movable gate defines an area for carrying the flow of chemical materials.2. The motorized laboratory hand-rotatable stopcock plug of claim 1 , wherein the electrically-operated motor comprises a stepper motor.3. The motorized laboratory hand-rotatable stopcock plug of claim 1 , wherein the electrically-operated motor comprises a Direct Current (D.C.) motor.4. The motorized laboratory hand-rotatable stopcock plug of claim 1 , wherein the position of the ...

SYSTEM AND CARTRIDGE FOR EFFICIENT NUCLEIC ACID TESTING

A disposable and inexpensive biological diagnostic cartridge for the amplification and detection of nucleic acids includes a configuration having a reaction pouch for amplification which is compressed by a flexible pump pouch for detection of the amplified reaction. 1. A biological diagnostic cartridge comprising:an inlet;a flexible reaction pouch downstream of the inlet;a flexible pump pouch adapted to compress the flexible reaction pouch; anda detection chamber downstream of the flexible reaction pouch.2. The cartridge of claim 1 , further comprising:a first fluid path from the inlet to the flexible reaction pouch, anda second fluid path from the flexible reaction pouch to the detection chamber.3. The cartridge of claim 2 , wherein each of the first and second fluid paths is one-directional.4. The cartridge of claim 1 , further comprising a pump adapted to inflate the flexible pump pouch.5. The cartridge of claim 4 , further comprising a housing.6. The cartridge of claim 5 , further comprisinga housing; anda pump adapted to inflate the flexible pump pouch, wherein the housing comprises a port for receiving the pump.7. The cartridge of claim 6 , wherein the pump comprises an electrolytic pump adapted to generate gas.8. The cartridge of claim 1 , further comprising an overflow pool adjacent to the detection chamber.9. The cartridge of claim 1 , wherein the pump pouch overlays the flexible reaction pouch.10. The cartridge of claim 1 , further comprising a one-way valve downstream of the reaction pouch and upstream of the detection chamber.11. The cartridge of claim 1 , further comprising at least a flow strip situated in the detection chamber.12. The cartridge of further comprising a vent downstream of the inlet and upstream of the reaction pouch.13. The cartridge of claim 12 , wherein the vent comprises a hydrophobic barrier.14. The cartridge of claim 1 , wherein the pump pouch contains a fluid.15. The cartridge of claim 1 , further comprising a second inlet claim 1 ...

FLUID-TIGHT FLOW SYSTEM TO ISOLATE BIOMARKERS FROM A LIQUID SAMPLE

The presently disclosed subject matter provides a fluid-tight flow system; a method of isolating biomarker cells from a liquid sample; a method of extracting cfDNA, ctDNA, or exosomes from plasma; a method of extracting cfDNA, ctDNA, or exosomes from blood; a method of capturing DNA or RNA released from biomarker cells; a method of amplifying a nucleotide sequence; a method of sequencing a nucleotide sequence; a method of detecting a chromosomal abnormality; and a method of analyzing a protein. 1. A fluid-tight flow system comprising:a microfluidic chip comprising an inlet port in fluid communication with an outlet port;a first automated pipetting channel comprising a first pump, and a first pipette tip containing a liquid sample and coupled to the inlet port;a second automated pipetting channel comprising a second pump, and a second pipette tip coupled to the outlet port; anda non-transitory computer readable medium in communication with the first pump and the second pump, and programmed to command the first pump of the first automated pipetting channel and the second pump of the second automated pipetting channel to control flow of the liquid sample through the microfluidic chip.2. The fluid-tight flow system of claim 1 , wherein the first pump or the second pump comprises a plunger and a pipetting drive motor.3. The fluid-tight flow system of claim 1 , wherein the first pump or the second pump comprises a piston contained within the first pipette tip and a pipetting drive motor.4. The fluid-tight flow system of claim 1 , wherein the system further comprises closed-loop feedback control wherein:the first automated pipetting channel further comprises a first pressure sensor;the second automated pipetting channel further comprises a second pressure sensor; andthe non-transitory computer readable medium is further in communication with the first pressure sensor and second pressure sensor; wherein said non-transitory computer readable medium is further programmed to ...

Liquid Evaluation

A liquid evaluation system can include a cartridge including a channel configured to pull a liquid into the channel by capillary action. The cartridge can include a first plate and a second plate located in close proximity to the first plate. An internal facing surface of each plate can include a corresponding region forming the channel. Each of the regions can have an affinity for the liquid. The close proximity of the plates and the regions having an affinity for the liquid cause the liquid to be pulled into the channel by capillary action. The cartridge can include one or more additional attributes and/or the system can include one or more additional components for performing the evaluation. 1. A liquid evaluation system comprising: a first plate including a first region of an internal facing surface forming a first side of the channel and a first side of an entrance region for the channel; and', 'a second plate including a second region of an internal facing surface forming a second side of the channel and a second side of the entrance region for the channel, wherein the first region and the second region are configured to have an affinity for the liquid, and wherein a separation between the first plate and the second plate causes the liquid to be pulled into the entrance region for the channel and the channel by capillary action, wherein the entrance region for the channel has a larger depth than a depth for the channel at an entrance of the channel located immediately adjacent to the entrance region for the channel., 'a cartridge including a channel for holding liquid, the cartridge including2. The system of claim 1 , wherein one of: the first plate or the second plate claim 1 , extends further than the other of: the first plate or the second plate claim 1 , beyond the entrance region for the channel located between the first plate and the second plate claim 1 , to assist with dispensing the liquid.3. The system of claim 1 , wherein at least one of: the first ...

Methods and apparatus to facilitate gravitational cell extraction

The invention relates generally to methods and apparatus that gravitationally transfer cells from a first medium to a second medium. More specifically, the invention relates to a novel microfluidic device. The microfluidic device includes a cell transfer region, a cell settling channel, a waste channel, a cell output channel, and an input medium channel. The cell settling channel, the waste channel, and the cell output channel extend from, are in fluid communication with, and are smaller in cross section than the cell transfer region. The cell output channel is substantially perpendicular to the cell settling channel and to the waste channel. The input medium channel extends from and is in fluid communication with the cell output channel.

OPTOFLUIDIC DIAGNOSTICS SYSTEM

An optofluidic diagnostic system and methods for rapid analyte detections. The system comprises an optofluidic sensor array, a test plate and an optical detection cartridge. The sensor array supports one or more distinct sensor units, each having a reactor section designed to temporarily enter a series of different kinds of wells in the test plate. One kind of well is a sample reservoir that holds reagent solution to be transferred into the reactor section. Another kind of well is a drainage chamber that removes reagent solution from the reactor section. A third kind of well is a colorant reservoir that holds a colorant reagent transferable into a reactor section. Finally, the sensor unit is transferred to the optical detection cartridge where it is placed into an isolation booth during the optical detection process so that its flat observation face is stationed in a viewing window opposite an optical detector lens. 1. A multi-well test plate for an optofluidic diagnostic system configured to interact with at least one sensor unit moved sequentially into and out of registry therewith , said test plate comprising:a plurality of wells, each said well having a well depth defined by an upper mouth and a lower base, each said well in said plate having a generally equal well depth,at least three wells being arranged in a sequence cluster, at least one well in said sequence cluster comprising a sample reservoir containing a liquid analyte reagent, at least one well in said sequence cluster comprising a drainage chamber configured to drain liquid reagents from a sensor unit, and at least one well in said sequence cluster comprising a colorant reservoir containing a liquid color development reagent.2. The test plate of claim 1 , wherein said sequence cluster includes a plurality of said sample reservoirs and a corresponding plurality of said drainage chambers claim 1 , said plurality of sample reservoirs disposed in alternating fashion with said plurality of drainage ...

REACTION APPARATUS AND TEMPERATURE CONTROL METHOD

The reaction apparatus includes a cell in which a specimen liquid of 1 μL or more is accommodated, a cooling plate structure that encloses the cell and consists of a cooling plate, a cooling unit that cools the cooling plate structure, and a heating unit that irradiates at least one of the cell or the specimen liquid with electromagnetic waves, in which the cooling plate configuring the cooling plate structure is in contact with at least two main surfaces of the cell, and the cooling plate structure includes a transparent window that transmits the electromagnetic waves. 1. A reaction apparatus , comprising:a cell in which 1 μL or more of a specimen liquid is accommodated;a cooling plate structure that encloses the cell and comprises a cooling plate;a cooling unit that cools the cooling plate structure;a heating unit that irradiates at least one of the cell or the specimen liquid with electromagnetic waves; anda control unit that controls irradiation of the electromagnetic waves by the heating unit, wherein:the cooling plate configuring the cooling plate structure is in contact with at least two main surfaces of the cell,the cooling plate structure includes a transparent window that transmits the electromagnetic waves,the control unit controls cooling by the cooling unit, andthe control unit performs control such that the specimen liquid is heated to a first temperature by the heating unit in a state in which a temperature of the cooling plate structure is maintained by the cooling unit at a second temperature that is at least 20° C. lower than the first temperature.2. The reaction apparatus according to claim 1 , wherein the cooling unit comprises a Peltier element.3. The reaction apparatus according to claim 1 , wherein the heating unit comprises a light source that emits light having a wavelength of from 0.5 μm to 1.4 μm as the electromagnetic waves.4. The reaction apparatus according to claim 1 , further comprising a temperature sensor that detects a temperature ...

CELL TREATMENT APPARATUS

A cell treatment apparatus capable of treating cells in a cell culture vessel. The cell treatment apparatus () according to the present invention includes a first region (), a second region (), and a third region (). The first region () and the second region () are placed in succession. The first region () is a cell treatment chamber for treating cells. The cell treatment chamber can be closed from the outside of the cell treatment chamber and includes a culture vessel placement portion for placing a cell culture vessel. The second region () includes a laser irradiation device capable of irradiating the cell culture vessel placed in the culture vessel placement portion with a laser. The third region () includes a control device that controls at least one device in the cell treatment apparatus () and a power supply device () that supplies electric power to at least one device in the cell treatment apparatus (). The culture vessel placement portion is placed to be adjacent to the second region () in the cell treatment chamber. An adjacent portion to the second region () in the culture vessel placement portion is translucent. 1. A cell treatment apparatus comprising:a first region;a second region; anda third region, whereinthe first region and the second region are placed in succession,the first region is a cell treatment chamber for treating cells,the cell treatment chamber can be closed from the outside of the cell treatment chamber and comprises a culture vessel placement portion for placing a cell culture vessel,the second region comprises a laser irradiation device capable of irradiating the cell culture vessel placed in the culture vessel placement portion with a laser,the third region comprises a control device that controls at least one device in the cell treatment apparatus and a power supply device that supplies electric power to at least one device in the cell treatment apparatus,the culture vessel placement portion is placed to be adjacent to the second ...

Active composite materials for micro-fluidic and nano-fluidic devices

Microfluidic devices in applications such as biological and chemical sensing provide high sensitivity and exploit minute amounts of samples and reagents in solutions that are low cost, compact, portable and easily distributed to end-users, etc. Polydimethylsiloxane (PDMS) is a common material for the mechanical structure of the body, micro-channels etc. However, its inert nature and biocompatibility mean that active functionality must be added after the formation of the microfluidic structures and structural elements. It would be beneficial to provide designers of microfluidic devices with the ability to implement active elements for the detection of one or more components within a fluid or arising within a fluid from a reaction upstream using a composite comprising a mechanical component, such as the PDMS, with the active material for the active element of the microfluidic device embedded with the PDMS as a composite.

Integrated specimen cup system for tamperproof sample testing and secure retention

A specimen cup system includes a cup defining first and second reservoirs in fluid communication with one another. The first reservoir has an open top with spaced-apart upper and lower annular flanges defined at the open top's external periphery. A test card is permanently disposed in the second reservoir. A base, rotatably coupled to the cup, defines a chamber having an opening therein. The opening of the chamber is in fluid communication only with the first reservoir when the base is in a first position, and is in fluid communication only with the second reservoir when the base is in a second position. A seal, rim, and lid cooperate with the upper and lower annular flanges of the open top of the first reservoir to seal the first reservoir after a specimen has been deposited therein.

Nose simulator with multisampling modes for airstream evaluation

A nasal simulator includes a three-dimensional (3D) printed nasal cavity within based on diagnostic imagery of a human nasal cavity. The nasal simulator comprising a fan system positioned to mimic air flow through the human nasal cavity. A first probe access bore is formed through the 3D printed nasal cavity to a first location having a first internal contour. An anemometer insert having an outer diameter sized to be slidingly received in and to pneumatically seal the first probe access bore, the anemometer insert having a distal contour that aligns with the first internal contour of the 3D printed nasal cavity, the anemometer insert having a longitudinal bore that is sized to receive a probe of an anemometer to detect characteristics of the air flow through the 3D cavity. 1. A nasal simulator comprising:a three-dimensional (3D) printed nasal cavity within based on diagnostic imagery of a human nasal cavity;a fan system positioned to mimic air flow through the human nasal cavity;a first probe access bore formed through the 3D printed nasal cavity to a first location having a first internal contour; andan anemometer insert having an outer diameter sized to be slidingly received in and to pneumatically seal the first probe access bore, the anemometer insert having a distal contour that aligns with the first internal contour of the 3D printed nasal cavity, the anemometer insert having a longitudinal bore that is sized to receive a probe of an anemometer to detect characteristics of the air flow through the 3D cavity.2. The nasal simulator of claim 1 , wherein the first probe access bore and the anemometer insert comprise a teeth and groove lock.3. The nasal simulator of claim 1 , further comprising:a second probe access bore formed through the 3D printed nasal cavity to a second location; anda resting insert having an outer diameter sized to be slidingly received in and to pneumatically seal the second probe access bore, and the resting insert having a distal contour ...

OPTICAL ANALYTE DETECTION

Analyte collection and testing systems and methods, and more particularly to testing systems and methods that achieve significant improvements in the detection of fluorescence signals in the reader by modulating the applied optical excitation. Also described herein are optical detection apparatuses and methods for removable photonic chips that do not require translation for calibration when coupling the photonics chip with the sensing system. Also described herein are methods and apparatuses for accurately calibrating a dilution factor when reading from a photonics chip. 1. A method of reading optical signals from a photonic chip of a removable cartridge held in an optical reader , the method comprising:modulating a pulsed excitation signal for a laser of the optical reader with a shift signal having a frequency of 1 kHz or greater to form a modulated signal;adjusting the peak height and duty cycle of the modulated signal to have an average power that is constant;exciting the laser with the modulated signal to illuminate the photonic chip;detecting an optical signal from the photonic chip;generating an output signal from the optical signal; anddemodulating the output signal using the modulated signal, wherein the demodulated signal is proportion to the cosine of the phase difference between the modulated signal and the output signal; andoutputting the demodulated output signal.2. The method of claim 1 , wherein the shift signal has a frequency of 10 kHz or greater.3. The method of claim 1 , further comprising low-pass filtering the demodulated output signal before outputting the demodulated output signal.4. The method of claim 2 , further comprising applying a matched filter to the demodulated output signal.5. The method of claim 1 , wherein the average power of the modulated signal approximately the same as an average power of the excitation signal.6. The method of claim 1 , wherein adjusting the peak height and duty cycle comprises adjusting the duty cycle to be ...

DEVICE AND METHOD FOR MEASUREMENT OF SARS-CoV-2 SPECIFIC ANTIGEN IN A BIOLOGICAL SAMPLE

A device for retaining a biological sample, for measuring a concentration of a SARS-CoV2 specific antigen, with SARS-CoV2 antigen-specific and electrochemically active immunoreceptor that is conjugated with an electrochemically active substance and optionally including an electrode reactivity enhancement agent and antibody stabilization agent. The immunoreceptor is configured to be in chemical contact with electrodes and a biological sample with SARS-CoV2 specific antigen of the device. The present invention also provides a device holder for holding the device of the present invention and a point-of-care biosensor. A method for measuring a concentration of SARS-CoV2 specific antigen from a reduced volume of biological sample is also provided in the presence of the antigen-specific and electrochemically active immunoreceptor, by measuring a peak value of redox current of the SARS-CoV2 antigen-specific and electrochemically active immunoreceptor and determining a concentration of SARS-CoV2 specific antigen in the biological sample, by linearly matching with a corresponding reference redox current. 1100. A device for collecting and retaining a biological sample , for measuring a concentration of a SARS-CoV2 specific antigen in a biological sample , comprising:{'b': ['102', '102', '101'], 'i': ['a', 'b'], '#text': '(i) at least a pair of conductive tracks , are disposed on a substrate ;'}{'b': ['103', '103', '102', '102'], 'i': ['a', 'b', 'a', 'b'], '#text': '(ii) at least a pair of electrodes , are connected to the at least pair of conductive tracks , ; and'}{'b': ['105', '103', '103'], 'i': ['a', 'b'], '#text': '(iii) a SARS-CoV2 antigen-specific and electrochemically active immunoreceptor that is conjugated with at least an electrochemically active substance, is configured to be in chemical contact with the at least pair of electrodes , and the biological sample.'}2100. The device as claimed in claim 1 , wherein the material for the substrate is a rigid material ...

Shock Tube and Cell Electroporation Device with the Shock Tube

The shock tube comprises a tube, a first electrode, a second electrode and a stopple, wherein the tube is internally provided with a cavity for accommodating a target liquid sample. The first electrode is arranged at one end of the tube. The second electrode is arranged in the stopple, and the outer end of the second electrode can be electrically connected with the exterior via an opening of the stopple. The stopple is internally provided with an elastic piece connected with the second electrode. The outer side of the elastic piece is connected with the stopple, and the inner side of the elastic piece is connected with the second electrode. The invention further provides a cell electroporation device where the shock tube can be placed. 120. A cell electroporation device for electroporation of cells in a biological sample in a shock tube () , comprising:{'b': 7', '7', '8', '8', '821, 'a housing (), the housing () disposed with a fixing base (), the fixing base () having a socket ();'}{'b': 9', '7', '9', '821, 'a cover () provided on the housing (), the cover () capable of covering an outer end of socket ();'}{'b': 27', '7', '27', '10', '11, 'a power module () within the housing (), the power module () electrically connected to the first electrode terminal () and the second electrode terminal ();'}{'b': 10', '821, 'a first electrode terminal () at an inner end of the socket (); and'}{'b': 11', '9, 'a second electrode terminal () at the cover ();'}{'b': 20', '2', '3, 'wherein the shock tube () having a first electrode () and a second electrode (); and'}{'b': 20', '821', '9', '10', '11', '2', '3', '20, 'wherein the shock tube () is placed in the socket () and when the cover () is closed, the first electrode terminal () and the second electrode terminal () are electrically connected to the first electrode () and the second electrode () to allow formation of an electric field within the shock tube () and to enable the injection of extracellular substances into the cells ...

COAGULATION AND AGGREGATION REFRACTED LIGHT INDEXING DEVICE AND METHOD

The present invention related to a device suitable for measuring an alteration of the state of a sample, comprising: a housing having a hollow interior, a light source positioned within the interior of the housing, a sensor positioned within the hollow interior of the chamber, wherein the sensor is able to measure the light of the interior of the housing, and a computing system connected to the light source and the sensor. 1. A device suitable for measuring an alteration of the state of a sample , comprising:a housing having a hollow interior;a light source positioned within the interior of the housing;a sensor positioned within the hollow interior of the housing; anda computing system connected to the light source and the sensor.2. The device of claim 1 , wherein a first portion of a surface of the hollow interior of the housing claim 1 , wherein the first portion of the surface has a reflective layer.3. The device of claim 1 , wherein a second portion of the surface of the hollow interior of the housing claim 1 , wherein the first portion of the surface has a low reflective coating.4. The device of claim 3 , wherein the light source is directed at the low reflective coating.5. The device of claim 1 , further comprising:a sample cartridge receiver positioned within the housing and between the light source and the sensor.6. The device of claim 1 , further comprising:a cover attached to the housing, wherein the cover is removable to allow access to the interior of the housing.7. The device of claim 1 , further comprising:an opening accessible from the exterior of the housing, wherein the opening is sized to accept a specimen.8. The device of claim 7 , further comprising:a set of guide rails, wherein the guide rails are sized and positioned to receive the specimen.9. The device of claim 1 , further comprising:a set of guide rails, wherein the guide rails are sized and positioned to receive the specimen and are accessible with the cover removed.10. The device of claim ...

DEVICE FOR MEASURING MICROFLUID FLOW VELOCITY BY USING ULTRA THIN FILM, HAVING SEPARABLE STRUCTURE

Provided is a device for measuring a microfluid flow velocity, having a structure separable by an ultra-thin film, the device including a first panel including a flow velocity measurement structure configured to measure a flow velocity of a fluid, a second panel configured to be separated from the first panel and including a microfluid channel through which a sample passes, and the ultra-thin film formed in a portion where the first panel and the second panel adjoin each other, the ultra-thin film being configured to separate the first panel and the second panel so that the sample passing through the microfluid channel does not come into direct contact with the flow velocity measurement structure, in which the first panel including the flow velocity measurement structure is usable multiple times repeatedly. 1. A device for measuring a microfluid flow velocity , the device comprising:a first panel comprising a flow velocity measurement structure configured to measure a flow velocity of a fluid;a second panel configured to be separated from the first panel and comprising a microfluid channel through which a sample passes; andan ultra-thin film formed in a portion where the first panel and the second panel adjoin each other, the ultra-thin film being configured to separate the first panel and the second panel so that the sample passing through the microfluid channel does not come into direct contact with the flow velocity measurement structure, wherein the first panel comprising the flow velocity measurement structure is usable multiple times repeatedly.2. The device of claim 1 , wherein the flow velocity measurement structure comprises:a heater configured to apply heat to the sample passing through the microfluid channel; andtwo temperature measuring electrodes installed at front and rear sides of the heater to measure a resistance difference in accordance with a change in temperature of the sample when the temperature of the sample is increased by the heat generated ...

AUTOMATED VOLUMETRIC DEVICE

The present invention concerns the technical field of volumetric measurement and volumetric instruments and provides novel devices and methods for the determination of the volume of a fluid. 230321011. The volumetric device of claim 1 , wherein the quantizing device () is a capillary valve () normally closed and drivable to open by individual pressure pulses applicable to the fluid () claim 1 , thereby releasing each one droplet () of uniform partial volume upon one or more pressure pulse applied.3. The volumetric device of claim 1 , further comprising:{'b': 26', '20', '10, 'a holder () for receiving the vessel () containing the fluid ().'}45-. (canceled)640. The volumetric device of claim 1 , wherein the droplet detector () is selected from the group consisting of: light detectors claim 1 , photoelectric barriers claim 1 , capacitive sensors and piezoelectric sensors and combinations of any two or three thereof.7. The volumetric device of claim 1 , further comprising:{'b': 70', '11', '30', '70, 'a receiver vessel () for receiving the plurality of droplets () released from the from the quantizing device (), the receiver vessel () optionally being detachable from the volumetric device.'}8. The volumetric device of claim 1 , further comprising:{'b': 80', '10', '11', '11, 'a microcontroller (), programmed to calculate the total volume of the fluid () from the total number of droplets () of partial volume of said fluid, given the predetermined partial volume of each droplet ().'}920322220. The volumetric device of claim 1 , wherein the vessel () is in the form of a disposable container claim 1 , selected from the group comprising reaction tubes claim 1 , vials claim 1 , cups and multiwell-plates claim 1 , and which comprises a capillary valve () that is integrated into the bottom tip () of the vessel ().1010. A method of volumetric measurement of the total volume of a fluid () claim 1 , comprising the steps of:{'b': 10', '20, 'a) receiving the fluid () to be metered in ...

Device, System And Method For Processing A Sample

A device for the processing of a sample comprises a location apparatus, a processing chamber for receiving the sample and a plurality of reagent chambers. The reagent chambers have openings defined in the location apparatus. The processing chamber is movable relative to the reagent chambers to enable sequential communication with each reagent chamber in turn.

SYSTEM FOR CAPTURING CELLS

The present disclosure relates to a cell extraction device comprising: a plurality of cell extraction modules arranged in a single layer array, the single layer array having a cell-receiving side wherein an opening of at least one of the plurality of cell extraction modules on the cell-receiving surface is configured to receive and retain a single target cell from a fluid sample, and a fluid-evacuating side wherein an opening of the or each of the plurality of cell extraction modules on the fluid-evacuating side is configured to allow fluid from the fluid sample to be evacuated from the cell extraction device, wherein at least one of the plurality of cell extraction modules comprises a micropore capillary configured at a first end that is open on the cell-receiving side to receive a target cell, and configured at a second end that is open on the fluid-evacuating side to allow fluid to pass through. 1. A cell extraction device comprising:a plurality of cell extraction modules arranged in a single layer array, the single layer array having a cell-receiving side wherein an opening of at least one of the plurality of cell extraction modules on the cell-receiving surface is configured to receive and retain a single target cell from a fluid sample, and a fluid-evacuating side wherein an opening of the or each of the plurality of cell extraction modules on the fluid-evacuating side is configured to allow fluid from the fluid sample to be evacuated from the cell extraction device,wherein at least one of the plurality of cell extraction modules comprises a micropore capillary configured at a first end that is open on the cell-receiving side to receive a target cell, and configured at a second end that is open on the fluid-evacuating side to allow fluid to pass through.2. The cell extraction device of claim 1 , wherein the single layer array is arranged over the surface of a planar disc claim 1 , and the surface is provided with a hydrophilic surface configuration.3. The cell ...

CELL ANALYSIS SYSTEMS

In one example in accordance with the present disclosure, a cell analysis system is described. The cell analysis system includes at least one cell analysis device. Each cell analysis device includes a channel to serially feed individual cells from a volume of cells into a lysing chamber. The cell analysis device also includes at least one feedback-controlled lysing element in the lysing chamber to agitate a cell. The cell analysis system also includes a controller to analyze the cell. The controller includes a lysate analyzer to analyze properties of the lysate and a rupture analyzer to analyze parameters of an agitation when a cell membrane ruptures. 1. A cell analysis system , comprising:{'claim-text': ['a channel to serially feed individual cells from a volume of cells into a lysing chamber;', 'at least one feedback-controlled lysing element in the lysing chamber to agitate a cell;'], '#text': 'at least one cell analysis device, each cell analysis device comprising:'}{'claim-text': ['a lysate analyzer to analyze properties of a lysate of the cell; and', 'a rupture analyzer to analyze parameters of an agitation when a cell membrane ruptures.'], '#text': 'a controller to analyze the cell, the controller comprising:'}2. The cell analysis system of claim 1 , wherein the lysing chamber further comprises:a sensor to determine whether the cell membrane was ruptured; anda return pump to re-direct a cell to the lysing chamber when the cell membrane has not ruptured.3. The cell analysis system of claim 1 , wherein each cell analysis device further comprises an encapsulator to encapsulate the lysate.4. The cell analysis system of claim 1 , further comprising:a cell reservoir fluidically coupled upstream of each cell analysis device to hold the volume of cells; anda waste reservoir fluidically coupled downstream of each cell analysis device to collect waste fluid.5. The cell analysis system of claim 1 , wherein each cell analysis device further comprises an ejector to eject ...

Digital Assay

Provided herein is a method and device for partitioning a fluidic sample. The device contains a plate containing microwells. The method comprises depositing a sample on one or both of the plates when the plates are in an open configuration, wherein the deposition is in the form of a single or multiple droplet of the sample, wherein at least one of the droplets has a volume that occupies more than two microwells and closing the plates to the closed configuration to partition the sample in the microwells. 1. A device for performing a digital assay comprising: (a) the first and second plates are movable relative to each other into different configurations including an open configuration and a closed configuration, and have, on their respective surface, a sample contact area for contacting a fluidic sample that contains an analyte;', 'and', '(b) the second plate has, in the sample contact area, the microwells, wherein each microwell has (i) a predetermined and known geometry, (ii) a well depth of 200 um or less, and (iii) a volume substantially less than that of the fluidic sample;'}, '(c) the spacers are on one or both of the plates, wherein the spacer has a pillar shape and uniform height;, 'a first plate, a second plate, spacers, and microwells, whereinwherein in an open configuration, the average spacing between the inner surface of the first plate and a rim of the microwells in the second plate is larger than the depth of the well and the sample is deposited on one or both of the plates; andwherein in a closed configuration, which is the configuration after the sample is deposited in the open configuration; in the closed configuration, at least a part of the sample is inside the microwells, and the average spacing between the inner surface of the first plate and the rim of the microwells in the second plate is regulated by the spacers to a spacing of ½ (one half) of the microwell depth or less.2. An apparatus comprising a thermal cycler and the device of .3. An ...

Microfluidic device, microfluidic system and method for the isolation of particles

A microfluidic device for the isolation of particles of at least one specific type of a sample is described. The microfluidic device comprises: a first inlet adapted to receive a sample comprising the particles of the specific type, at least part of a separation group comprising a separation unit, which comprises a main chamber and a recovery chamber and being adapted to receive the sample and to transfer at least part of the particles of the specific type from the main chamber to the recovery chamber in a selective manner with respect to further particles of the sample, a first outlet which is designed to allow the particles of the specific type to be collected outside of the device and a second outlet adapted to allow at least a portion of the sample to flow out of the main chamber and out of the microfluidic device.

ON-SITE DETECTION OF PARASITIC INFECTION OF MAMMALS

A portable kit for generating a digital image of a faecal sample suitable for microscopic analysis, comprises a faecal sample preparation device configured to receive a faecal sample and a faecal flotation fluid, filter a suspension comprising the faecal sample and the faecal flotation fluid to provide a filtrate, a translucent faecal sample support, and a portable digital imaging module. The portable digital imaging module comprises a housing, a camera/microscopic lens assembly configured to generate a digital image of the faecal sample on the sample support, an illumination system, a seat for receiving the faecal sample support disposed between the camera/microscopic lens assembly and illumination system, a memory for storing the digital image, a communication system for communicating the digital image to an off-site image processing module via a communications network, and a battery operatively connected to the camera and microscopic lens assembly, memory and communication system. 1. A system for on-site determination of a parasitic infection characteristic of a non-human mammal , comprising:a portable faecal sample preparation device configured for use on-site to receive faecal matter from the mammal and a faecal flotation fluid, mix the faecal matter and faecal flotation fluid to provide a suspension, and filter the suspension to provide a filtrate, in which the filtrate is the faecal sample;a translucent faecal sample support;a portable digital imaging module adapted to generate a digital image of a faecal sample on the sample support and communicate the digital image via a wireless communications network by detachable engagement with a mobile communications device in a sample support imaging position, in which the digital image of the faecal sample on the sample support is generated by a camera of the mobile communications device and communicated to the off-site digital image analysis module via the mobile communications device;an off-site digital image ...

RAPID AND HIGH-PRECISION SIZING OF SINGLE PARTICLES USING PARALLEL SUSPENDED MICROCHANNEL RESONATOR ARRAYS AND DECONVOLUTION

Systems and methods for measuring the properties (e.g., masses, weights, densities, etc.) of particles, such as biological entities, in a fluidic channel are generally provided. In some embodiments, the systems and methods comprise a plurality of suspended microchannel resonators (SMRs) configured to operate simultaneously. A particle or a plurality of particles may be dissolved or suspended in a fluid, whereby the fluid is flowed through an inlet (e.g., an inlet channel) that is fluidically connected in parallel and in fluid communication with at least one SMR (e.g. at least one SMR, at least two SMRs, at least four SMRs, at least 8, at least 16 SMRs). Fluid containing a particle or particles may flow into the plurality of SMRs, which may oscillate at a certain frequency (e.g., a resonance frequency). As particles pass through the SMR(s), the mass of particle may cause a change in the resonance frequency, the change in frequency which may be read out via embedded piezoresistors. The SMR may comprise a cantilever, where shifts in the resonance frequency of each cantilever can be tracked independently and whereby frequency-multiplexing allows each cantilever to be continuously driven at the resonance frequency using a single actuation channel and a single detection channel. This may provide a precise, statistically-relevant property determination of the particles within the fluid (e.g., the mass of the particles). 1. A fluidic system , comprising:an inlet;a plurality of suspended microchannel resonators each comprising a fluidic channel connected fluidically in parallel and in fluidic communication with the inlet;an excitation element for driving one or more of the suspended microchannel resonators;a sensor associated with the plurality of suspended microchannel resonators, wherein the plurality of suspended microchannel resonators is configured to be operated essentially simultaneously.2. A method of determining a property of a particle , comprising:flowing the ...

Systems and methods for facilitating fluid flow during enhanced detection and quantification of analytes

Devices, systems, and methods for detecting molecules of interest within a collected sample are described herein. In certain embodiments, self-contained sample analysis systems are disclosed, which include a reusable reader component, a disposable cartridge component, and a disposable sample collection component. The reader component may communicate with a remote computing device for the digital transmission of test protocols and test results. In various disclosed embodiments, the systems, components, and methods are configured to identify the presence, absence, and/or quantity of particular nucleic acids, proteins, or other analytes of interest, for example, in order to test for the presence of one or more pathogens or contaminants in a sample.

Cartridges, collectors, kits, and methods for enhanced detection and quantification of analytes in collected fluid samples

Devices, systems, and methods for detecting molecules of interest within a collected sample are described herein. In certain embodiments, self-contained sample analysis systems are disclosed, which include a reusable reader component, a disposable cartridge component, and a disposable sample collection component. The reader component may communicate with a remote computing device for the digital transmission of test protocols and test results. In various disclosed embodiments, the systems, components, and methods are configured to identify the presence, absence, and/or quantity of particular nucleic acids, proteins, or other analytes of interest, for example, in order to test for the presence of one or more pathogens or contaminants in a sample.

METHODS AND APPARATUS FOR MEASURING ANALYTES USING LARGE SCALE FET ARRAYS

Methods and apparatus relating to very large scale FET arrays for analyte measurements. ChemFET (e.g., ISFET) arrays may be fabricated using conventional CMOS processing techniques based on improved FET pixel and array designs that increase measurement sensitivity and accuracy, and at the same time facilitate significantly small pixel sizes and dense arrays. Improved array control techniques provide for rapid data acquisition from large and dense arrays. Such arrays may be employed to detect a presence and/or concentration changes of various analyte types in a wide variety of chemical and/or biological processes. In one example, chemFET arrays facilitate DNA sequencing techniques based on monitoring changes in the concentration of inorganic pyrophosphate (PPi), hydrogen ions, and nucleotide triphosphates. 1. A method of using a semiconductor device , the method comprising:flowing a reagent solution uniformly through a flow cell of an apparatus, the apparatus comprising:a substrate including an array of sensors;an array of wells disposed over the substrate, each well capacitively coupled to at least one sensor of the array of sensors, each well of array of wells forming a reaction chamber for a chemical reaction; anda flow cell cover disposed over the array of wells to cover the flow cell and defining a flow volume between the array of wells and the flow cell cover, the cover defining a fluid inlet and a fluid outlet in fluid communication with the flow volume, the flow cell including a diffuser providing uniform fluid flow over the sensor array device; andmeasuring a signal from each sensor of the array of sensors in response to a reaction in a reaction chamber.2. The method of claim 1 , wherein a nucleic acid is disposed within the well and the reagent solution includes a nucleotide claim 1 , wherein measuring the signal includes measuring a byproduct released as a result of nucleotide incorporation during an extension reaction.3. (canceled)4. The method of claim 2 ...

SYSTEMS AND METHODS FOR SINGLE-MOLECULE NUCLEIC-ACID ASSAY PLATFORMS

Integrated circuits for a single-molecule nucleic-acid assay platform, and methods for making such circuits are disclosed. In one example, a method includes transferring one or more carbon nanotubes to a complementary metal-oxide semiconductor (CMOS) substrate, and forming a pair of post-processed electrodes on the substrate proximate opposing ends of the one or more carbon nanotubes. 1. A method of making an integrated circuit for a single-molecule nucleic-acid assay platform , comprising:transferring one or more carbon nanotubes to a complementary metal-oxide semiconductor (CMOS) substrate; andforming a pair of post-processed electrodes on the substrate proximate opposing ends of the one or more carbon nanotubes.2. The method of claim 1 , wherein transferring the one or more carbon nanotubes comprises spinning the one or more carbon nanotubes from a suspension to the substrate.3. The method of claim 1 , wherein transferring the one or more carbon nanotubes comprises:forming the one or more carbon nanotubes on a transfer substrate;applying a layer of polymer to the transfer substrate to adhere the one or more carbon nanotubes to the transfer substrate; andplacing the layer of polymer with the one or more carbon nanotubes on the CMOS substrate.4. The method of claim 1 , wherein transferring the one or more carbon nanotubes comprises:placing the one or more carbon nanotubes in a suspension proximate a pair of preformed electrodes on the substrate; andapplying a voltage across the pair of preformed electrodes, whereby a force is applied to the one or more carbon nanotubes to urge the carbon nanotubes to be disposed across the pair of preformed electrodes.5. The method of claim 1 , wherein the substrate comprises surface-exposed electrodes claim 1 , and forming the pair of post-processed electrodes comprises depositing titanium on a pair of the surface-exposed electrodes.6. The method of claim 1 , wherein the substrate comprises surface-exposed electrodes claim 1 , and ...

POLYMERASE CHAIN REACTION SYSTEM

A Polymerase Chain Reaction (PCR) device used to detect infectious agents and certain diseases. The device provides a quantitative PCR that amplifies the target DNA sequence, while monitoring the amplitude of signal originating from DNA-intercalating probes, typically fluorescence, to quantify the amount of DNA being amplified. The device is used in combination with other medical devices to obtain the user's physical exam data. A combination of the PCR machine and above-mentioned other instruments with a network communication device (TOTUS) sends the physical exam and PCR information to servers, and/or physicians, pharmacists and other medical professionals to provide AI decisions.

SYSTEM AND METHOD FOR SERIAL PROCESSING OF MULTIPLE NUCLEIC ACID ASSAYS

The present invention relates to systems and methods for the real time processing of nucleic acid during polymerase chain reaction (PCR) and thermal melt applications. According to an aspect of the invention, a system for the rapid serial processing of multiple nucleic acid assays is provided. In one embodiment, the system includes, but is not limited to: a microfluidic cartridge having microfluidic (flow-through) channels, a fluorescence imaging system, a temperature measurement and control system; a pressure measurement and control system for applying variable pneumatic pressures to the microfluidic cartridge; a storage device for holding multiple reagents (e.g., a well-plate); a liquid handling system comprising at least one robotic pipettor for aspirating, mixing, and dispensing reagent mixtures to the microfluidic cartridge; systems for data storage, processing, and output; and a system controller to coordinate the various devices and functions. 1. A system for serial processing of nucleic acid assays , comprising:a microfluidic cartridge comprising an interface chip having at least one inlet port and microfluidic channel and a reaction chip having at least one microfluidic channel in fluid communication with an associated microfluidic channel of the interface chip; (a) draw a first fluid into the microfluidic channel of the interface chip via the inlet port of the interface chip;', '(b) create a first fluid segment in the microfluidic channel of the reaction chip by drawing the first fluid from the associated microfluidic channel of the interface chip into the microfluidic channel of the reaction chip;', '(c) draw a second fluid into the microfluidic channel of the interface chip via the inlet port of the interface chip while maintaining the position of the first fluid segment in the reaction chip; and', '(d) create a second fluid segment in the microfluidic channel of the reaction chip by drawing the second fluid from the associated microfluidic channel of ...

SYSTEM FOR IMPLEMENTING BIOLOGICAL OR CHEMICAL METHODS

The invention having functional vertical disposable reaction systems having a vertically mounted semipermeable membrane for sample preparation, chemical reactions, dialysis, enzymatic/microbiological fermentation, multistage processes, in vitro protein biosynthesis on a laboratory scale, formed from a base body and an exchangeable lid having different functions. For exchange across the membrane, the system is placed vertically into an outer volume consisting of gas, liquid or solid constituents. The system consists of a dimensionally stable base body and a liquid-tight lid having a functional support going toward the base of the base body, the dimensionally stable base body forming at least one noncapillary reaction space as inner volume with at least one semipermeable membrane as lateral wall. The high flexibility in use results from the combination of variants of the base bodies with different lid variants for different areas of use. The base bodies having different membranes and volumes can be coupled with lids having different feeding openings, contacts, sensor supports, gas supply means, circulation means, etc. This yields, in the case of m different base bodies and n different lid variants, m×n combinations having different properties. 1. A system for carrying out biological or chemical methods , comprising at least one base body and at least one separately provided lid which is matched with the base body such that lid and base body can form a firm connection with one another ,wherein the base body comprises at least one structural element and at least one membrane and the membrane at least sectionally borders an inner volume of the base body as a lateral wall running substantially in parallel to the longitudinal axis of the base body,wherein the lid comprises at least one functional support which is, at its proximal end, connected to a sealing section of the lid and comprises, in the region of its distal end, one or more functional features,wherein the inner ...

Methods for Screening and Subsequent Processing of Samples Taken from Non-Sterile Sites

A method of analyzing a sample comprising one or more species of microorganisms can include generating first droplets such that each of one or more microorganisms of a first portion of the sample is encapsulated within one of the first droplets and, for each of one or more aliquots of a second portion of the sample, second droplets such that each of one or more microorganisms of the aliquot is encapsulated within one of the second droplets. First and second sets of data can be captured, the first set indicative of the identity and quantity of encapsulated microorganism(s) of the first portion of the sample and the second set indicative of a phenotypic response of encapsulated microorganism(s) of the aliquot(s) to one or more test reagents. A target species' phenotypic response to the test reagent(s) is determinable at least by referencing the second data set to the first data set. 1. A method of analyzing a sample comprising one or more species of microorganisms , the method comprising:generating, with a first device, a plurality of first droplets from a first liquid that comprises a first portion of the sample such that each of one or more microorganisms of the first portion of the sample is encapsulated within one of the first droplets;capturing, with one or more sensors, a first set of data indicative of the identity and quantity of the encapsulated microorganism(s) of the first portion of the sample;identifying at least one of the one or more species of the sample as a target species based on the first set of data;for each of one or more aliquots of a second portion of the sample, generating, with a second device, a plurality of second droplets from a second liquid that comprises the aliquot such that each of one or more microorganisms of the aliquot is encapsulated within one of the second droplets;for at least one of the aliquot(s), introducing a test reagent into at least some of the second droplets;capturing, with one or more sensors, a second set of data ...

A DEVICE AND METHOD FOR HIGH-THROUGHPUT MULTIPARAMETER MEASUREMENTS IN ONE OR MORE LIVE AND FIXED CELLS

A microfluidic device includes a first substrate including at least one microfluidic channel and a plurality of microwells, as well as a cooperating second substrate defining multiple split-walled cell trap structures that are registered with and disposed within the plurality of microwells. A method for performing an assay includes flowing cells and a first aqueous medium into a plurality of microwells of a microfluidic device, wherein each microwell includes a cell trap structure configured to trap at least one cell. The method further comprises flowing a nonpolar fluid with low permeability for analytes of interest through a microfluidic channel to flush a portion of the first aqueous medium from the microfluidic channel while retaining another portion of the first aqueous medium and at least one cell within each microwell. Surface tension at a non-polar/polar medium interface prevents molecule exchange between interior and exterior portions of microwells. 1. A microfluidic device comprising:a first substrate defining at least one microfluidic channel and a plurality of microwells; anda second substrate defining a plurality of split-walled cell trap structures, wherein the plurality of split-walled cell trap structures is registered with and disposed within the plurality of microwells.2. The microfluidic device of claim 1 , further comprising a gap between the first substrate and the second substrate along a lip of each microwell of the plurality of microwells.3. The microfluidic device of claim 1 , wherein each cell trap structure of the plurality of split-walled cell trap structures comprises an open upstream end sized to receive at least one cell claim 1 , and comprises a downstream opening configured to inhibit passage of at least one cell while permitting passage of an aqueous medium.4. The microfluidic device of claim 3 , wherein the at least one microfluidic channel comprises an increased lateral dimension proximate to each microwell of the plurality of ...

METHOD AND DEVICE FOR HIGH-THROUGHPUT SOLUTION EXCHANGE FOR CELL AND PARTICLE SUSPENSIONS

A method of exchanging fluids with suspended particles includes providing a microfluidic device with a first inlet channel operatively coupled to a source of particles and a second inlet channel operatively coupled to an exchange fluid. A transfer channel is connected at a proximal end to the first inlet channel and the second inlet channel. First and second outlet channels are connected to a distal end of the transfer channel. The source of particles is flowed at a first flow rate into the first inlet channel while the exchange fluid is flowed at a second flow rate into the second inlet channel wherein the ratio of the second flow rate to the first flow rate is at least 1.5. Particles are collected in one of the first and second outlet channels while fluid substantially free of particles is collected in the other of the first and second outlet channels. 133-. (canceled)34. A microfluidic system for solution exchange comprising:a first inlet channel operatively coupled to a source of particles suspended in a fluid;a second inlet channel operatively coupled to an exchange fluid;a linear and longitudinally symmetrical transfer channel having a proximal end and a distal end, the proximal end of the transfer channel connected to the first inlet channel and the second inlet channel;a plurality of outlet channels connected to a distal end of the transfer channel; and;{'sub': 'eq', 'a first pump configured to pump the source of particles suspended in a fluid at a first flow rate and a second pump configured to pump the exchange fluid at a second flow rate, wherein the ratio of the second flow rate to the first flow rate is at least 1.5 and wherein the exchange fluid occupies more than half of the transfer channel and wherein inertial lift forces in the transfer channel direct the particles from the source of particles to an equilibrium position (X) located within a center of the transfer channel and within the exchange fluid.'}35. The microfluidic system of claim 34 , ...

MULTI-FACTOR URINE TEST SYSTEM THAT ADJUSTS FOR LIGHTING AND TIMING

System that enables urine testing in a home environment. A user may apply a urine sample to a card containing multiple tests, and capture an image of the card using a phone; an analysis system executing on the phone or in the cloud may analyze the image and determine test results. The test card and analysis system may compensate for variability in lighting conditions and time of exposure to the urine sample, which are difficult to control in a home environment. The test card may contain fiducial markers of known colors; the analysis system may adjust colors in the captured image based on appearance of these markers. Color adjustments may also compensate for nonuniform lighting across the card. The card may also contain time indicators that change appearance over time after urine is applied, and the analysis system may use these indicators to calculate the time of exposure. 1. A multi-factor urine test system that adjusts for lighting and timing , comprising:{'claim-text': [{'claim-text': ['wherein said plurality of test regions comprise either lateral flow assay regions or colorimetric test regions or both;', {'claim-text': ['react with one or more substances that may be present in a urine sample to which each test region is exposed; and', 'change appearance based on a presence of or a quantity of the one or more substances in the urine sample;'], '#text': 'wherein each test region comprises one or more reagents configured to'}], '#text': 'a plurality of test regions,'}, {'claim-text': 'corner fiducial markers located at a boundary of an area of the test card within which the plurality of test regions are contained;', '#text': 'a plurality of fiducial markers, each fiducial marker of the plurality of fiducial markers comprising a reference color that is measured under a reference lighting condition, and wherein the plurality of fiducial markers comprises'}], '#text': 'a test card comprising'}{'claim-text': ['receive an image of the test card captured after the test ...

TEST CARTRIDGE FOR ANALYSIS INSTRUMENTS

A test cartridge for insertion into an analysis instrument having an actuation mechanism, the test cartridge having one or more sensors for measuring an aspect of the actuation mechanism. 1. A test cartridge for insertion into an analysis instrument having a linear actuation mechanism , a rotational actuation mechanism , a heater , a pump actuation mechanism , a second linear actuation mechanism , and a cartridge receiver , the test cartridge comprising:a test cartridge housing having a form factor to be received by the cartridge receiver of the analysis instrument;a plurality of first linear translation sensors, each first linear translation sensor having first detector and a corresponding first movable element linearly translatable along a first axis, wherein at least a first portion of each of the plurality of first linear translation sensors is positioned within the test cartridge housing such that the corresponding first movable element thereof is to engage with the linear actuation mechanism of the analysis instrument when the test cartridge is inserted into the analysis instrument and the linear actuation mechanism of the analysis instrument is actuated, and wherein each first detector of each of the plurality of first linear translation sensors is to detect a first distance moved by the corresponding first movable element along the first axis relative to the test cartridge housing responsive to a linear input provided by the linear actuation mechanism of the analysis instrument when the test cartridge is inserted into the analysis instrument and the linear actuation mechanism of the analysis instrument is actuated;a second linear translation sensor having a second detector and a second movable element linearly translatable along a second axis, wherein at least a second portion of the second linear translation sensor is positioned within the test cartridge housing such that the second movable element is to engage with the pump actuation mechanism of the ...

MICROFLUIDIC CHIP, SYSTEMS, AND METHODS FOR CAPTURING OF ENVIRONMENTAL DNA

A microfluidic lab-on-a-chip (LOC) device, microfluidic systems, and associated methodology are described that allow for intelligently collecting environmental DNA (eDNA) and their associated metadata. Optical spectroscopy is integrated with filtration membranes on the microfluidic device. The microfluidic LOC device and systems can be used for selectively capturing targeted species based on optical characteristics and for recording relevant metadata on eDNA acquired by the filtration membranes. 1. A microfluidic system , comprising:an environmental sample inlet port;a preservative reagent inlet port;a waste outlet port;one or more discrete filter membranes for particle concentration;at least one fluid access port that permits control of the sample fluid stream through a selected filter membrane;at least one optical flow cell that enables at least one of light microscopy, fluorescence spectroscopy, light attenuation measurements, and scattered light intensity measurements, situated before the one or more filter membranes to interrogate the incoming sample fluid stream; andat least one bypass channel to enable disposal of the incoming fluid sample stream and avoid capturing suspended microbes and/or particles on a filter membrane.2. The microfluidic system of claim 1 , wherein the microfluidic system comprises a microfluidic chip having a plurality of layers claim 1 , comprising:an upper layer defining the environmental sample inlet port, the preservative reagent inlet port, the waste outlet port, and in fluid communication with at least one intermediate layer;an intermediate layer in fluidic communication with the one or more filter membranes; anda lower layer in fluid communication with the intermediate layer, incorporating the at least one fluid access port, and the at least one optical flow cell in optical communication with an instrumentation housing.3. The microfluidic system of claim 1 , wherein the at least one optical cell comprises at least one of an ...

MICROFLUIDIC CHIPS WITH OPTICALLY TRANSPARENT GLUE COATING AND A METHOD OF MANUFACTURING MICROFLUIDIC CHIPS WITH OPTICALLY TRANSPARENT GLUE COATING FOR A MICROFLUIDIC DEVICE

A microfluidic chip for a microfluidic system includes a PDMS substrate having a first thickness, at least one microfluidic pathway in the substrate, a coating along the microfluidic pathway, and a glass layer having a second thickness on the substrate and above the microfluidic pathway, wherein the coating contains an optically transparent material, and the first thickness is greater than the second thickness. The coating includes cyanoacrylates, an UV curable epoxy adhesive, a gel epoxy or epoxy under trade name of EPO-TEK OG175, MasterBond EP30LV-1 or Locite 0151. 1. A microfluidic system comprising:a microfluidic chip configured to receive a fluid sample for an optical analysis, the microfluidic chip including at least one microfluidic pathway for the fluid sample to flow through, the at least one microfluidic pathway including a coating to reduce fluid diffusion; andan optical sensor configured to perform the optical analysis of the fluid sample while the fluid sample is held within the microfluidic chip.2. The microfluidic system of claim 1 , wherein the optical sensor is configured to measure a fluorescence of the fluid sample while the fluid sample is held within the microfluidic chip.3. The microfluidic system of claim 1 , wherein the optical sensor is configured to perform the optical analysis of the fluid sample while the fluid sample is located within the at least one microfluidic pathway.4. The microfluidic system of claim 1 , wherein the microfluidic chip includes a substrate and a glass layer claim 1 , the at least one microfluidic pathway formed between the substrate and the glass layer.5. The microfluidic system of claim 4 , wherein the at least one microfluidic pathway is etched into the substrate between the substrate and the glass layer.6. The microfluidic system of claim 1 , wherein the coating includes an acrylic resin.7. The microfluidic system of claim 1 , wherein the coating includes cyanoacrylates.8. The microfluidic system of claim 1 , ...

CELL ANALYSIS SYSTEMS WITH CELL ENCAPSULATION

In one example in accordance with the present disclosure, a method is described. The method includes receiving, in a microfluidic channel, serially fed cells to be encapsulated. Each cell is individually lysed and a lysate of each cell is transported to a downstream analysis device. The lysate of an individual cell is encapsulated with an encapsulating fluid. 1. A method , comprising:receiving, in a microfluidic channel, serially fed cells to be encapsulated;individually lysing each cell;transporting a lysate of each cell to a downstream analysis device; andencapsulating the lysate of an individual cell with an encapsulating fluid.2. The method of claim 1 , wherein:transporting the lysate of each cell to a downstream analysis device comprises ejecting the lysate through an orifice; andencapsulating the lysate of an individual cell comprises ejecting the lysate towards a well of a well plate that is filled, at least in part, with the encapsulating fluid.3. The method of claim 1 , wherein the encapsulating fluid comprises:an oil that is immiscible with the lysate; andsurfactant mixed with the oil.4. The method of claim 1 , further comprising adding a densification agent to the cell prior to lysis claim 1 , wherein the densification agent increases an overall density of the cell and encapsulating fluid mixture.5. The method of claim 1 , further comprising adding a densification agent to the lysate following lysis claim 1 , wherein the densification agent increases an overall density of the lysate and encapsulating fluid mixture.6. A cell analysis device claim 1 , comprising:a microfluidic channel to serially transport cells along a flow path;a lysis device along the microfluidic channel to rupture a cell membrane of a cell; anda number of encapsulation inlets to deliver a quantity of an encapsulating fluid into the microfluidic channel to encapsulate a lysate of the cell.7. The cell analysis device of claim 6 , further comprising at least one of:a number of secondary ...

NUCLEIC ACID DETECTION

The present disclosure relates to nucleic acid detection devices. The nucleic detection device can include a microfluidic architecture to receive biological fluid, a multimodal sensor bundle, a common transmission line to transmit an enhanced current signal generated by combining current signals from individual sensors, and a current to voltage converter to receive and convert the enhanced current signal to voltage. The multimodal sensor bundle can be positioned to interact with the biological fluid when present within the microfluidic architecture. The multimodal sensor bundle can include multiple types of sensors selected from an electrochemical sensor, an optical sensor, or a potentiometric sensor. Individual sensors of the multimodal sensor bundle independently can generate a current signal in response to interaction with the biological fluid. 1. A nucleic acid detection device , comprising:a microfluidic architecture to receive biological fluid;a multimodal sensor bundle positioned to interact with the biological fluid when present within the microfluidic architecture, wherein the multimodal sensor bundle includes multiple types of sensors selected from an electrochemical sensor, an optical sensor, or a potentiometric sensor, wherein individual sensors of the multimodal sensor bundle independently generates a current signal in response to interaction with the biological fluid;a common transmission line to transmit an enhanced current signal generated by combining current signals from the individual sensors; anda current to voltage converter to receive and convert the enhanced current signal to voltage.2. The nucleic acid detection device of claim 1 , further comprising a sensor mask associated with the multimodal sensor bundle claim 1 , wherein the sensor mask is electrically associated with the multimodal sensor bundle to enable or disable the individual sensors of the multimodal sensor bundle.3. The nucleic acid detection device of claim 1 , wherein the ...

Mems affinity sensor for continuous monitoring of analytes

Techniques for monitoring a target analyte in a sample using a polymer capable of binding to the target analyte are disclosed. A microdevice useful for the disclosed techniques includes a semi-permeable membrane structure, a substrate, a first and second microchambers formed between the membrane structure and the substrate. The first microchamber can be adapted to receive a solution including the polymer, and the second microchamber can be adapted to receive a reference solution. Environmental target analyte can permeate the semi-permeable membrane structure and enters the first microchamber and the second microchamber. Based on the difference in a property associated with the polymer solution that is responsive to the target analyte-polymer binding, and the corresponding property associated with reference solution, the presence and/or concentration of the target analyte can be determined.

Processing Cartridge and Method for Detecting a Pathogen in a Sample

In one embodiment, a multiplex fluid processing cartridge includes a sample well, a deformable fluid chamber, a mixing well with a mixer disposed therein, a lysis chamber including a lysis mixer, an electrowetting grid for microdroplet manipulation, and electrosensor arrays configured to detect analytes of interest. An instrument for processing the cartridge is configured to receive the cartridge and to selectively apply thermal energy, magnetic force, and electrical connections to one or more discrete locations on the cartridge and is further configured to compress the deformable chamber(s) in a specified sequence. 1. A method of motivating a sample through a cartridge , the method comprising:(a) motivating the sample on a first substrate using microfluidics; and(b) motivating the sample on a second substrate using electrowetting, thereby motivating a sample through a cartridge, wherein (a) is not accomplished by electrowetting.2. The method of claim 1 , further comprising claim 1 , after (b) claim 1 , motivating the sample into an electrosensor array located on the second substrate.3. The method of claim 1 , wherein (a) comprises pumping the sample from a first location to a second location.4. The method of claim 1 , wherein (a) comprises compressing a fluid-filled deformable compartment supported on the first substrate claim 1 , thereby generating pressure to move the sample.5. The method of claim 1 , wherein (a) comprises introducing magnetic beads to the sample and applying a magnetic force to move the sample.6. The method of claim 1 , wherein (a) comprises introducing negatively-charged capture beads to the first substrate to facilitate adsorption of positively-charged nucleic acids from the sample to a surface of the negatively-charged capture beads claim 1 , thereby moving the sample.7. The method of claim 1 , further comprising introducing process fluids into the first substrate by compressing a fluid-filled deformable compartment supported on the first ...

Apparatuses and Methods for Operating a Digital Microfluidic Device

Described herein are apparatuses and methods for the processing and/or measurements of chemical or biochemical samples on a digital microfluidic device. Also described are methods to configure and operate the modules for efficient processing and measurements of the samples on the device. The apparatus can be used in applications such as DNA/RNA/protein/cell concentration/purification, real-time PCR, isothermal amplification, immunoassay, cell-based assay, library preparation for NGS sequencing, etc. 1. An apparatus for operating at least one digital microfluidic (DMF) device , and performing processing and/or analyzing of one or more biologic sample(s) on said device(s) , which comprises:a) at least one voltage control module to provide electric signals to the DMF device for the manipulation of the liquids/droplets on said device(s); andb) at least one magnet control module which controls one or more magnets, wherein each magnet has at least two degrees of freedom of movement (or two independent movement directions).2. The apparatus of claim 1 , wherein one degree of freedom is to move the magnet(s) close to the DMF device to produce a magnetic field at a specified location on the device or to move the magnets away far enough that insignificant or zero magnetic field is produced at any location on the DMF device.3. The apparatus of claim 2 , wherein the degree of freedom of the movement is a linear motion and is perpendicular to the DMF device.4. The apparatus of claim 2 , wherein the magnet can be brought to as close as making a physical contact with the DMF device.5. The apparatus of claim 1 , wherein at least one magnet is a focusing magnet.6. The apparatus of claim 1 , wherein all the magnets are controlled by the same magnet control module claim 1 , so that the magnets always move along the same direction and by the same distance.7. The apparatus of claim 1 , further comprising at least one temperature control module with at least one temperature control ...

PROCESS FOR SORTING CELLS BY MICROFABRICATED COMPONENTS USING A NUCLEASE

The invention is directed to a process for sorting target cells and non-target cells from a sample by a cell sorting valve microfabricated on a surface of a silicon substrate, with microfabricated channels leading from the cell sorting valve, wherein the cell sorting valve separates the target particles from non-target material; a disposable cartridge containing a sample reservoir, a sort reservoir and a waste reservoir; wherein the sample is provided in a buffer comprising nuclease. 1. Process for sorting target cells and non-target cells from a sample by a cell sorting valve microfabricated on a surface of a silicon substrate , with microfabricated channels leading from the cell sorting valve , wherein the cell sorting valve separates the target particles from non-target material;a disposable cartridge containing a sample reservoir, a sort reservoir and a waste reservoir;wherein the sample is provided in a buffer comprising nuclease.2. Process according to claim 1 , characterized in that the nuclease is cation-independent.3. Process according to claim 1 , characterized in that the nuclease is cation-independent and the buffer contains a chelating agent capable of forming complexes with cations.4. Process according to claim 1 , characterized in that the nuclease is cation-dependent.5. Process according to claim 1 , characterized in that the nuclease is a DNAse.6. Process according to claim 1 , characterized in that the nuclease is non-specific.7. Process according to claim 1 , characterized in that an interposer provides fluid communication between the microfabricated channels in the silicon substrate and the reservoirs in the disposable cartridge.8. Process according to claim 7 , characterized in that the interposer provides a sort fluid path between a sort reservoir in the disposable cartridge and the sort channel in the silicon substrate claim 7 , a waste fluid path between a waste reservoir in the disposable cartridge and the waste channel claim 7 , and a ...

METHOD AND A SYSTEM FOR QUANTITATIVE OR QUALITATIVE DETERMINATION OF A TARGET COMPONENT

A method and a system for quantitative or qualitative determination of a target component in a liquid sample. The method includes i) providing a plurality of magnetic particles including capture sites for the target component on their respective surfaces; ii) providing a plurality of fluorophores configured to bind to the capture sites of the magnetic particles; iii) bringing the liquid sample into contact with the fluorophores and the magnetic particles in a flow channel of a micro fluidic device including a transparent window; and iv) at least temporally immobilizing the magnetic particles adjacent to the transparent window using a magnet, emitting exciting electromagnetic beam towards the immobilized magnetic particles, reading signals emitted from fluorophores and performing a quantitative or qualitative determination of the target component based on the read signal. A suitable micro fluidic device for use in the method/system and a kit for preparing a liquid sample. 1. A system for quantitative or qualitative determination of a target component in a liquid sample , the system comprisesa micro fluidic device comprising at least one flow channel with a transparent window and an inlet for the liquid sample;a plurality of magnetic particles comprising capture sites for the target component on their respective surfaces;a plurality of fluorophores configured to bind to said capture sites of the magnetic particles or to said target component;a magnet arranged to at least temporally immobilize said magnetic particle adjacent to said transparent window;an emitter for exciting said fluorophores, anda reader for reading signals emitted from said fluorophores,wherein the micro fluidic device comprises a flexible wall section of the flow channel or of a sink section in fluid connection with the flow channel and the system comprises an actuator, which is adapted to move the flexible wall section for feeding a liquid sample into the flow channel.2. The system of claim 1 , ...

ASSAY DEVICE

Provided herein is technology relating to detecting one or more analytes in a sample and particularly, but not exclusively, to devices, methods, systems, and kits for detecting one or more drugs-of-abuse analytes in a biological sample. 1. An assay device comprising:a) a chamber configured for collecting and holding a sample, said chamber comprising an indication structure;b) a reservoir comprising a test device; andc) a non-rectangular sample passage slot fluidly connecting said chamber and said reservoir.2. The assay device of wherein said test device comprises 6 or more channels configured to hold 6 or more test strips.3. The assay device of wherein said non-rectangular sample passage slot has a trapezoidal or a triangular shape.4. The assay device of wherein said trapezoidal shape has a first base that is 20-40 mm long.5. The assay device of wherein said trapezoidal shape has a second base that is 5-25 mm long.6. The assay device of wherein said trapezoidal shape has a height of 2-15 mm.7. The assay device of wherein said trapezoidal shape is an isosceles trapezoid.8. The assay device of wherein said trapezoidal shape comprises two angles of 30 to 60 degrees.9. The assay device of wherein said non-rectangular sample passage slot has a shape that is an arch claim 1 , segment of a circle claim 1 , or segment of an ellipse.10. The assay device of further comprising a lid configured to seal said chamber.11. The assay device of wherein said indication structure is configured to produce a vibration when said lid is securely engaged with said chamber.12. The assay device of wherein a torque of at least 21.3 pound-inch is required to produce the vibration.13. The assay device of wherein said lid is a screw-lid.14. The assay device of wherein said test device further comprises a sample validity testing strip configured to test for at least three sample characteristics.15. The assay device of wherein said sample characteristics are pH claim 14 , specific gravity claim 14 ...

DISPOSABLE MICROFLUIDIC CASSETTES

A disposable microfluidic cassette can include a substrate and an engagement feature associated with the substrate to removably join the cassette with a cassette-receiver of an analytical system. A microfluidic network can be carried by the substrate. The microfluidic network can include a fluid inlet, a fluid outlet, and a sample manipulation portion fluidly coupling the fluid inlet to the fluid outlet. An ejector can be associated with the microfluidic network to move fluid out of the disposable microfluidic cassette via the fluid outlet. 1. A disposable microfluidic cassette , comprising:a substrate;an engagement feature associated with the substrate to join the cassette with a cassette-receiver of an analytical system;{'claim-text': ['a fluid inlet,', 'a fluid outlet, and', 'a sample manipulation portion fluidly coupling the fluid inlet to the fluid outlet; and'], '#text': 'a microfluidic network carried by the substrate, the microfluidic network, including:'}an ejector associated with the microfluidic network to move fluid out of the disposable microfluidic cassette via the fluid outlet.2. The disposable microfluidic cassette of claim 1 , wherein the engagement feature is removably joinable with the cassette-receiver of the analytical system.3. The disposable microfluidic cassette of claim 1 , wherein the microfluidic network has a volume of from 0.5 picoliters (pL) to 1 milliliter (mL).4. The disposable microfluidic cassette of claim 1 , further comprising a sample manipulation component associated with the sample manipulation portion claim 1 , the sample manipulation component selected from a temperature regulator claim 1 , a thermal cycler claim 1 , an ultrasonic transducer claim 1 , a piezoelectric appliance claim 1 , an integrated micropump claim 1 , a mixer claim 1 , a lyser claim 1 , a particle sorter claim 1 , a valve claim 1 , or a combination thereof.5. The disposable microfluidic cassette of claim 1 , further comprising a detector positioned to ...

MICROFLUIDIC DEVICE AND METHOD FOR RAPID HIGH THROUGHPUT IDENTIFICATION OF MICROORGANISMS

An apparatus and method are disclosed for detecting the presence of a microorganism within sampling device. The sampling device has a plurality of reaction chambers each having a reactive reagent for reacting with the microorganism to indicate the presence of the microorganism within the reaction chamber. A grabber holding the sampling device and a motion stage connected to the grabber moves the sampling device in a plane. A detector detects each of the plurality of reaction chambers for detecting the presence of the microorganism within the reaction chamber. 1. An apparatus for detecting a microorganism within sampling device , the sampling device having a plurality of reaction chambers each having a reactive agent for reacting with the microorganism to indicate the presence of the microorganism in said reaction chamber , comprising:a grabber for holding the sampling device;a motion stage connected to said grabber for moving the sampling device in a plane; anda detector for detecting each of the plurality of reaction chambers for detecting the presence of the microorganism in said reaction chamber.2. The apparatus for detecting the Microorganism within sampling device as set forth in claim 1 , wherein the sampling device is a rotatable disk having a central hole; andsaid grabber engaging with the central hole for holding and rotating said rotary disk.3. The apparatus for detecting the Microorganism within sampling device as set forth in claim 1 , wherein said detector is a spectrometer for detecting the presence of the microorganism in the reaction chamber.4. The apparatus for detecting the Microorganism within sampling device as set forth in claim 1 , wherein said detector includes a light source located on one side of the sampling device and a spectrometer located on another side of the sampling device for detecting the presence of the microorganism in the reaction chamber.5. The apparatus for detecting the Microorganism within sampling device as set forth in ...

Methods, devices, and systems for detecting two or more analytes within small volumes

This document provides methods, devices, and systems for detecting the presence, absence, or amount of two or more analytes present within a small volume (e.g., less than 10 μL) of a sample (e.g., a blood sample) obtained from a mammal (e.g., a human such as a human neonate). For example, methods and materials for using plasma separation and multiplex analyte detection to detect two or more analytes (e.g., proteins, carbohydrates, lipids, nucleic acids, intact cells, intact viruses, intact microorganisms, and/or chemicals) within a small volume of a blood sample are provided.

Opto-Fluidic Array for Radical Protein Foot-Printing

Systems and methods of in vivo and in vitro radical protein foot-printing using an opto-fluidic array are presented. These teachings may be used to, for example, study three-dimensional protein structure or bio-kinetics. Radical dosimetry including an optional intrinsic standard is used. Real-time feedback based on an internal standard provides comparability between different experiments and in vivo and in vitro analysis results in data that is representative of actual biological conditions. 1. An opto-fluidic chip for radical protein footprinting comprising:a substrate;a first port, a second port, and a third port each defined into the substrate;a mixing zone comprising a first cavity defined within the substrate and in fluid communication with both the second and third ports;a photolysis zone comprising a second cavity defined within the substrate and in fluid communication with the mixing zone, the photolysis zone further comprising a first optically transparent window sealed to the substrate and bounding one side of the second cavity, the second cavity comprising a channel configured in a serpentine pattern; the photolysis zone is transparent on both sides.a dosimetry zone comprising a third cavity defined within the substrate and in fluid communication with the photolysis zone and in further fluid communication with the first port, the dosimetry zone further comprising a second optically transparent window sealed to the substrate and bounding one side of the third cavity.2. The opto-fluidic chip of wherein the substrate comprises a plastic.3. The opto-fluidic chip of wherein the substrate comprises quartz.4. The opto-fluidic chip of wherein the substrate comprises a middle layer of silicon claim 3 , a top layer of quartz claim 3 , and a bottom layer of quartz claim 3 , the middle layer being disposed between the top and bottom layers.5. The opto-fluidic chip of wherein the substrate comprises a middle layer of silicon claim 3 , a top layer of fused silica claim ...

Cell analyzers

A system includes a microchannel analysis region, a first fluid actuation device, a second fluid actuation device, a sensor, and a controller. The first fluid actuation device is at a first end of the microchannel analysis region. The second fluid actuation device is at a second end of the microchannel analysis region opposite to the first end. The sensor is within the microchannel analysis region between the first fluid actuation device and the second fluid actuation device. The sensor measures an impedance of a fluid within the microchannel analysis region. The controller activates the first fluid actuation device to generate a first pressure wave in the fluid and activates the second fluid actuation device to generate a second pressure wave in the fluid. The first pressure wave and the second pressure wave converge at the sensor.

Devices, systems, and methods for controlling liquid flow

Disclosed are devices, systems, kits, and methods for controlling liquid flow and, e.g., in particular, for forming droplet having substantially uniform droplet-to-droplet content. The devices, systems, and kits may include a first channel including a funnel or may include a first channel and a first-side channel, the first channel being in fluid communication with a droplet formation region. The devices, systems, and kits may further include a second channel fluidically connected to the first channel or the first side-channel. Funnels and/or side-channels may be used to enhance the control over particle spacing in the channels, thereby providing superior control over the number of particles of the same kind in formed droplets. The devices, systems, and kits of the invention may further include a mixer downstream of a channel intersection. Mixers can be used to reduce localized pockets of high concentration of dissolved ingredients.

SYSTEMS AND METHODS FOR SINGLE CELL ISOLATION AND ANALYSIS

The present disclosure relates to devices, systems, and methods for single cell isolation and analysis. In particular, the present disclosure relates to systems and methods for isolating single cells present at low numbers. 1. A system , comprising:a) a cell capture device comprising a plurality of wells, wherein each well is configured to capture a single cell, and wherein each well comprises a transport channel and a cell culture chamber;b) a fluid transport device configured to transport fluid through the transport channel of said cell capture device without dead volume.2. The system of claim 1 , wherein said cell capture device comprises at least 100 wells.3. The system of claim 1 , wherein said cell capture device comprises at least 5000 wells.4. The system of claim 1 , wherein said cell capture device comprises at least 10 claim 1 ,000 wells.5. The system of claim 1 , wherein said system further comprises a microscope in optical communication with said cell capture device.6. The system of claim 1 , wherein said fluid transport device is a vacuum device.7. The system of claim 6 , wherein said vacuum device and said cell culture chamber is approximately 50 μm.8. The system of claim 1 , wherein said cell capture device is constructed of PDMS.9. The system of claim 1 , wherein said cell culture well is approximately 100-500 μm in length and width.10. The system of claim 1 , wherein said main channel is approximately 50 μm in width.11. The system of claim 1 , wherein said cell capture device is approximately 40 to 100 μm in height.12. The system of claim 1 , wherein said system is configured to capture 10 or less cells present in a volume of 10 μl or less.13. The system of claim 1 , wherein said cell culture chamber further comprises one or more sensors for sensing pH claim 1 , oxygen claim 1 , glucose claim 1 , proteins claim 1 , or metabolic byproducts.14. A method of capturing single cells claim 1 , comprising:a) contacting a solution comprising one or more ...

MICROFLUIDIC SUBSTRATE, DRIVING METHOD THEREOF, AND MICRO TOTAL ANALYSIS SYSTEM

A microfluidic substrate and the driving method for the microfluidic substrate are provided. The microfluidic substrate may include a base substrate () and a plurality of detecting modules () on the base substrate (). Each of the detecting modules () may include a switching unit (), a driving electrode (), and a photosensor (). The photosensor () may have a first terminal () and a second terminal (). A signal output terminal () of the switching unit () may be connected to both the driving electrode () and the second terminal () of the photosensor (). 1. A microfluidic substrate , comprising:a base substrate; anda plurality of detecting modules on the base substrate, each of the detecting modules comprising a switching unit, a driving electrode, and a photosensor having a first terminal and a second terminal, wherein a signal output terminal of the switching unit is connected to both the driving electrode and the second terminal of the photosensor.2. The microfluidic substrate according to claim 1 , wherein the first terminal and the second terminal of the photosensor are sequentially disposed in a direction away from the base substrate.3. The microfluidic substrate according to claim 2 , wherein the switching unit claim 2 , the first terminal claim 2 , a semiconductor junction claim 2 , and the second terminal of the photosensor claim 2 , and the driving electrode of each of the detecting modules are sequentially stacked on the base substrate;a first insulating layer is between the switching unit and the first terminal of the photosensor;a second insulating layer is between the semiconductor junction and the driving electrode;the signal output terminal of the switching unit is connected to the driving electrode through a first through hole in the first insulating layer and the second insulating layer; andthe driving electrode is connected to the second terminal of the photosensor through a second through hole in the second insulating layer.4. The microfluidic ...