ДРОЖЖЕВОЙ ШТАММ ДЛЯ ПОЛУЧЕНИЯ БИОМАССЫ НА СУБСТРАТЕ, СОДЕРЖАЩЕМ С5-САХАРА, И ЕГО ПРИМЕНЕНИЕ

Группа изобретений относится к вариантам дрожжевой клетки рода сахаромицетов, способной к размножению в аэробных условиях на субстрате, содержащем по меньшей мере один C5-сахар, и применению такой клетки. Дрожжевая клетка характеризуется тем, что удовлетворяет тесту роста на ксилозе или тесту роста на среде CP, а также тем, что в ее геноме экспрессия генов RPE1, RKI1, TKL1 и TAL1 пентозного пути помещена под контроль промотора гена, нерепрессируемого анаэробиозом или катаболической репрессией, индуцируемой любым источником углерода, и экспрессирующегося в ходе спиртовой ферментации, копии гена GRE 3, кодирующего альдозоредуктазу, удалены, нативный ген XKS1 сверхэкспрессирован, введена по меньшей мере одна копия гена Pichia stipitis, кодирующего ксилозодегидрогеназу, и введена по меньшей мере одна копия гена, кодирующего ксилозоизомеразу. Предложено также применение указанной дрожжевой клетки для изготовления хлеба. Группа изобретений обеспечивает получение дрожжей, обладающих высокой эффективностью ...

ГРАНУЛЫ, ИСПОЛЬЗУЕМЫЕ В КЛЕТОЧНОЙ КУЛЬТУРЕ, И СПОСОБЫ ИХ ПОЛУЧЕНИЯ

Группа изобретений относится к получению сухих клеточных культуральных сред или подпиток в формате гранул. Предложены способы получения гранул клеточной культуральной среды, модуля базового порошка или питательной среды путем прямого гранулирования. Вносят во вращающийся дисковый ротор аппарата с псевдоожиженным слоем первый сухой порошок клеточной культуральной среды, сухой базовый порошок питательной среды или сухой порошок питательной среды, где указанный первый сухой порошок предварительно смачивают. Далее вносят во вращающийся дисковый ротор растворитель и/или второй сухой порошок клеточной культуральной среды, сухой базовый порошок питательной среды или сухой порошок питательной среды, так, чтобы происходило образование гранулы. Первый сухой порошок, второй сухой порошок или растворитель содержит связующее вещество, вспомогательное вещество или их оба. Высушивают гранулы. Изобретения позволяют осуществлять прямое гранулирование порошков клеточных культуральных сред, подпиток или добавок ...

БИОЛОГИЧЕСКАЯ ОБРАБОТКА РУДЫ ДЛЯ ВЫДЕЛЕНИЯ ТЯЖЕЛЫХ МЕТАЛЛОВ

Изобретение относится к биологической обработке руды для выделения или обогащения. Способ идентификации бактерии, способной связываться с тяжелым металлом в виде золота, серебра, рутения, родия, палладия, осмия, иридия, платины и/или редкоземельного металла, включает культивирование исследуемой бактерии в контейнере с пригодной первой культуральной средой в течение первого периода времени, с обеспечением пригодной плотности ее клеток. Часть поверхности исследуемого инструмента, на который нанесено покрытие из тяжелого металла, погружают в первую среду в течение второго периода времени, достаточного для обеспечения прикрепления исследуемой бактерии к погруженной поверхности упомянутого инструмента. После чего удаляют исследуемый инструмент из контейнера с первой культуральной средой. Проводят контактирование поверхности упомянутого инструмента со второй культуральной средой, содержащей указанный металл, для высевания упомянутой бактерии во вторую культуральную среду при ее прикреплении к ...

ФЕРМЕНТАТИВНЫЙ ГИДРОЛИЗ ЦЕЛЛЮЛОЗЫ

... 1. Способ непрерывного гидролиза целлюлозной биологической массы, включающий, по меньшей мере, следующие стадии:(P) обеспечения, по меньшей мере, одного реактора, который может работать в стационарном состоянии;(A) добавления заданного количества целлюлозной биологической массы в указанный реактор, при этом указанная целлюлозная биологическая масса имеет содержание твердых продуктов, по меньшей мере, 10%;(Α') добавления заданного количества ферментов в указанный реактор;(E) осуществления, по меньшей мере, частичного ферментативного гидролиза целлюлозной биологической массы в указанном реакторе,при этом достигается стационарное состояние, при котором целлюлозную биологическую массу непрерывно добавляют в указанный реактор, в то время как, по меньшей мере, частично гидролизованную целлюлозную биологическую массу непрерывно удаляют из указанного реактора, при этом указанная, по меньшей мере, частично гидролизованная целлюлозная биологическая масса, которую непрерывно удаляют, имеет вязкость ...

Verfahren zur Verzuckerung von Holz, Torf und anderen pflanzlichen Stoffen mit verduennten Saeuren

Culture media contg. vegetable matter and peat hydrolysates - for high biomass yield cultivation of fodder yeasts

Culture medium contains (by wt.) 48-97% hydrolysate of vegetable matter at pH 4-4.4; 0.1-50% peat hydrolysate at pH 4-4.4; 0.02-0.08% (as N) of N source; 0.03-0.12% (as P2O5) of P source; 0.16-0.64% (as K) of K source; and water to 100%. The medium is used for cultivation of fodder yeasts that act as vitamin-protein additives to low protein basic fodder for animals and birds. The yield of the biomass of fodder yeasts is improved (up to 70 wt. %).

Lignocellulose conversion processes and products

Bioconversion of agricultural wastes into proteinaceous animal feed

The present invention is directed to the conversion of agricultural waste material, including animal manure and crop wastes, by a fermentation process using edible microbial organisms, into proteinaceous animal feed products.

A process for fed-batch yeast propagation

PROCESSING BIOMASS

PROCESSING BIOMASS

PROCESSING BIOMASS

ACIDOPHILIC FUSARIUM OXYSPORUM STRAINS, METHODS OF THEIR PRODUCTION AND METHODS OF THEIR USE

Production of nutritive mediums for the culture of micro-organisms.

Process of rough sapogenin extraction of sheets of sisal plant.

Process of obtaining proteinic matters starting from agricultural waste.

PROCESSING BIOMASS

PROCEDURE FOR THE ENZYMATIC ACTIVATION FROM LIGNOCELLULOSEN FIBROUS MATERIALS TO THE PRODUCTION OF MATERIALS

PROCEDURE FOR THE ZUECHTEN OF BORDETELLA PERTUSSIS, A PERTUSSIS TOXOID AND A PERTUSSIS VACCINE.

Sirna-mediated gene silencing with viral vectors

Abstract The present invention is directed to viral vectors encoding small interfering RNA molecules (siRNA) targeted against a gene of interest, and methods of using these viral vectors ...

Enzymatic hydrolysis of cellulose

The present invention relates to a continuous process for the enzymatic hydrolysis of cellulosic biomass and to an apparatus for conducting said process. According to the present invention, a steady state is achieved in a reactor in regard to the hydrolysis reaction. Therein, cellulosic biomass of a high total solids content (preferably 10% or higher, further preferably between 15 and 30%) is continually added to said reactor, while at least partially hydrolyzed cellulosic biomass is continually removed from said reactor. The steady state is adjusted, i.e. the amount of cellulosic biomass added and the amount of at least partially hydrolyzed cellulosic biomass removed is adjusted, so that the retention time of a given portion of added cellulosic biomass in the reactor is longer than its "liquefaction time", i.e. the time period required to transform a solid slurry into a pumpable liquid during hydrolysis, i.e. the time required to lower the viscosity of the slurry to a value, which is acceptable ...

Processing biomass

Feedstocks, obtained at least in part from a plant material that has been modified with respect to its wild type, are processed to produce useful intermediates and products, such as energy, fuels, foods or materials. For example, systems are described that can treat such feedstock materials, e.g., to reduce the recalcitrance of the feedstock, and use the treated feedstock materials to produce an intermediate or product, e.g., by saccharification and/or fermentation.

Plant derived cell culture material

The present invention relates material that is useful in culturing and transferring cells as well as delivering cells. The material comprises plant derived cellulose nanofibers or derivatives thereof, wherein the cellulose nanofibers are in a form of a hydrogel or membrane. The invention also provides methods for producing these materials and compositions and uses thereof.

Yeast strains for the production of biomass on a substrate comprising a C5 sugar

Abstract: The present invention concerns novel Saccharomyces cerevisiae yeast strains capable of multiplying on a substrate comprising at least one C5 sugar with a speed and rate of multiplication compatible with the industrial production of yeast. It also concerns novel strains which, when cultured, make it possible to obtain yeasts having an application efficiency, i.e. an efficiency that is satisfactory in applications and uses of interest in industries such as breadmaking, biomass production, flavour production, the production of secondary metabolites, protein production, ethanol production, brewing, winemaking or the production of yeast extract.

METHOD FOR CULTIVATION OF MICROORGANISM

COMPARTMENTALIZED SIMULTANEOUS SACCHARIFICATION AND FERMENTATION OF BIOMASS

Methods and apparatus' are disclosed for the simultaneous saccharification and fermentation of biomass providing for the compartmentalization of the saccharification process and the fermentation process resulting in decreased enzymatic end-product inhibition.

HYPERPRODUCING CELLULASE MICROORGANISM

GLUCOSE AND XYLOSE CO-FERMENTING MICROORGANISM THAT EXPRESSES ACTIVE GLUCOAMYLASE

Provided are microorganisms, e.g., the Saccharomyces yeast, that have been made able to co-ferment xylose sugar obtained from hydrolyzing plant cellulosic biomass from trees, grasses, straws, etc., with glucose that can be obtained from hydrolyzing either edible feedstocks such as starch, cane sugar, etc., or from hydrolyzing cellulose from various types of non-edible cellulosic biomass. The microorganisms are also capable of expressing an amylase, e.g., glucoamylase, having nonnegligible enzymatic activity, capable of producing glucose from oligo- or polysaccharides obtained by treating soluble starch with a-amylase. In some embodiments, nucleotidic material is provided comprising genes actively expressing xylose reductase, xylitol dehydrogenase and xylulokinase, as well as an active gene expressing glucoamylase. Vectors and other compositions of matter are provided as well.

NON-NATURAL AMINO ACID REPLICATION-DEPENDENT MICROORGANISMS AND VACCINES

Compositions and methods of producing vaccines, including methods wherein whole organism vaccines are provided with limited replication abilities, thereby increasing vaccine safety and efficacy, through the use of non-natural, unnatural, or non-naturally encoded amino acids.

BIOMASS LIQUEFACTION PROCESSES, AND USES OF SAME

Described are processes for the liquefaction of lignocellulosic biomass under the digestive action of dicarboxylic acid(s). Such digests can exhibit enhanced flowability, reduced volume, and significant biomass conversion to dissolved components, and can in some embodiments be further liquefied by contact with an enzyme. Products resultant of these steps can be used for their sugar content to manufacture biofuels or other products.

ELIMINATING THE NEED OF ACIDIFICATION IN BIOETHANOL PRODUCTION

An improved ethanol fermentation process with decreased use of acidifiers by adding a composition containing an aldehyde, a fatty acid, a terpene and a surfactant. The method comprising: a) mixing a fermentation feedstock with a fermentation broth containing yeast and/or an enzyme, b) treating said mixture by adding a composition to the fermentor containing: - 90 wt. % of an antimicrobial aldehyde, preferably selected from the group consisting of formaldehyde, para-formaldehyde, glutaraldehyde, and mixtures thereof, 1 - 50 wt. % of a surfactant having an HLB from 4 to 18, 0 - 20 wt. % of an antimicrobial terpene, or essential oils, 1 - 50 wt. % of organic acids selected from C1 to C24 fatty acids, their salts, glycerides and esters thereof, and 1 - 50 wt. % water; wherein the concentration of aldehyde in the fermentor is from about 0.25 to 3 kg/MT of fermentation feedstock, and c) isolating ethanol and improving yield.

SOLID STATE CULTURE OF WHITE ROT FUNGI

... 2101057 9213960 PCTABS00014 White-rot fungi are grown on a sugar beet pulp substrate. By-products of fungal growth, such as lignin-degrading enzymes, can be recovered from the culture. The culture or enzymes recovered from the culture can be used to degrade aromatic compounds in bioremediation procedures.

Säuglingsunterlage für Kinderwagen oder dergleichen.

Verfahren zur Entsäuerung und Entbitterung von Hydrolyseprodukten zellulosehaltiger Stoffe.

Verfahren zur Herstellung von Futter- oder Nahrungsmitteln aus minderwertigen animalischen oder vegetabilischen Produkten.

Verfahren zur Kennzeichnung von Magnetogrammträgern.

Putzeinrichtung für die Zylinderstreckwerke von Spinnmaschinen.

Verfahren zur magnetischen Schallaufzeichnung bei Diktiergeräten.

Verfahren zur Erzeugung von Mirkoorganismen, insbesondere Hefe.

Verfahren zur Auflockerung der Zellen von gärbaren Stoffen.

Procédé d'épuration des eaux résiduaires provenant de la fabrication de la pâte à papier préparée par le procédé à la soude

Verfahren zur Gewinnung von Hefe aus den bei der Viskoseherstellung anfallenden alkalischen Celluloseanteilen.

Verfahren zur Herstellung von Eiweissstoffen aus den bei der Vorbehandlung zellulosehaltiger Rohstoffe mittels organischer oder anorganischer Säuren in Lösung gegangenen Zellulosebegleitstoffen.

Verfahren zum Verdünnen der für die Vorhydrolyse von zellstoffhaltigen Ausgangsstoffen mit verdünnten Säuren notwendigen Säure.

Richtantennenanordnung für Ultrakurzwellenbetrieb innerhalb eines breiten Frequenzbandes.

Kopiermaschine, insbesondere Kopierfräsmaschine.

Verfahren zur Herstellung von 1.5-Dichlornaphtalin-4.8-dicarbonsäure.

Yeast production for fodder

Yeast is cultured in a medium consisting of vegetable tissue hydrolysates or hydrocarbons of the n-paraffin series with aeration of the medium. Novelty is characterised by the introduction of Ozone during the prepn. of the medium and during the culture of the yeast. The O3 combats the multiplication of undesirable microorganisms.

Edible protein prodn. from cereals - by starch hydrolysis, ultrafiltration and aerobic fermentation

Parent patent describes the prodn. of proteins from protein-contg. starch and cellulose prods. by starch hydrolysis, ultra-filtration and aerobic fermentation. In this addition cereals of all types are used as raw material. Hydrolysis may be carried out with inorganic acid, weak alkali or enzymes, e.g. amylase, to produce dextrin and disaccharides. The solid residue from the ultra-filter is washed several times with water and given no further concn., contg. the native protein of the grain. Protein concentrates and yeasts suitable for human consumption are obtd. The raw materials being themselves edible.

Plant hydrolysate purification for use as fermentation substrate

Plant hydrolysate, used as nutrient substrate for microorganisms in simple cell protein prodn., is obtd. by hydrolysis with a dil. inorganic acid, esp. H2SO4, in a percolator. The plant hydrolysate is (i) treated wit pref. solid Ca(OH)2, (ii) separated from ppte., (iii) neutralised with CO2 and (iv) again separated from ppte. Pref. raw hydrolysis material is bagasse. Separation takes place by centrifuging and filtration.Humic substances are removed by co-pptn. with metal hydroxides. End-prod. is lighter in colour, i.e. yielding a lighter single cell protein after fermentation.

CELLULOSE FERMENTATION PROCESS.

STRAINS OF YEAST, CONSTRUCTED FOR PRODUCING ETHANOL FROM ACETIC ACID AND GLYCEROL

LACCASE AND THEIR APPLICATION

STRAINS OF YEAST, CONSTRUCTED FOR PRODUCING ETHANOL FROM ACETIC ACID AND GLYCEROL

SELECTION OF THE ORGANISMS, CAPABLE OF FERMENTING THE MIXED SUBSTRATA

МЯГКАЯ ЩЕЛОЧНАЯ ПРЕДВАРИТЕЛЬНАЯ ОБРАБОТКА И ОДНОВРЕМЕННОЕ САХАРООБРАЗОВАНИЕ И ФЕРМЕНТИРОВАНИЕ ЛИГНОЦЕЛЛЮЛОЗНОЙ БИОМАССЫ С ПОЛУЧЕНИЕМ ОРГАНИЧЕСКИХ КИСЛОТ

Настоящее изобретение относится к способу получения продукта ферментирования из лигноцеллюлозной биомассы, к реактору для осуществления способа и к использованию реактора для получения продукта ферментирования.

ФЕРМЕНТАТИВНОЕ ПОЛУЧЕНИЕ ОРГАНИЧЕСКИХ СОЕДИНЕНИЙ

Изобретение относится к способу получения как минимум одного органического соединения, которое содержит как минимум 3 атома углерода или как минимум 2 атома углерода и как минимум 1 атом азота, которое выбирают из моно-, ди- и трикарбоновых кислот, которые содержат от 3 до 10 атомов углерода, и, в случаи необходимости, содержат гидроксильные группы протеиногенных и непротеиногенных аминокислот, пуриновых оснований, пиримидиновых оснований; нуклеозидов, нуклеотидов, липидов; насыщенных и ненасыщенных жирных кислот; диолов, которые содержат от 4 до 10 атомов углерода, многоатомных спиртов, которые содержат 3 или более гидроксильных групп, длинноцепочечных спиртов, которые содержат как минимум 4 атома углерода, углеводов, ароматических соединений, витаминов, провитаминов, кофакторов, нутрицевтических средств, белков, каротиноидов, кетонов, которые содержат от 3 до 10 атомов углерода, лактонов, биополимеров и циклодекстринов путем ферментации, которая включает такие стадии: а1) измельчение ...

METHOD FOR PRODUCING SINGLE CELL OIL FROM LIGNOCELLULOSIC MATERIALS

Method for production of l-amino acid

In a method for producing an L-amino acid by fermentation comprising culturing a microorganism having an L-amino acid-producing ability in a liquid medium to accumulate the L-amino acid in the medium with precipitation of the L-amino acid, a polymer selected from the group consisting of a water-soluble cellulose derivative, a water-soluble polyvinyl compound, a polar organic solvent-soluble polyvinyl compound, a water-soluble starch derivative, an alginic acid salt and a polyacrylic acid salt is added to the medium.

COMPOSE OF the 2-MONO ACID TYPE OR POLY-CHLOROMETHYL-DITHIO-ACETIQUE AND ITS APPLICATION LIKE ADDITIVE TO FOOD OF the RUMINANTS

PROCESS AND DEVICE Of a TREATMENT UNIT BIOPHYSICS WITH REAGENTS OF RESONANCE INTENDED TO ACCELERATE AND OPTIMIZE the PROCESSES OF FERMENTATION OF the CELLULO-LIGNEUX

Improvements with the manufacture of the aliphatic acids and other products of fermentation

Order for machines to be reproduced, in particular for copying milling machines

A METHOD OF PRODUCING A SUBSTRATE SUITABLE FOR FERMENTATION

Treatment of crop products with high pressure, production of sugars, furfuryl alcohol, extracts tannic and feedingstuffs

Acid 1.5-dichloro-naphthalene-4.8-dicarboxylique, acid 1.5-dichloro-4.8-di- (omega-chloracétyle) - naphthalene and method of preparation of these products

The method of separating and recovering volatile acid, methylated products and body hyrdolyzed furfuraniques ligno-cellulose. (g. Meunier system)

PROCESS FOR ENZYMATIC HYDROLYSIS FROM A MIXTURE OF DIFFERENT POROSITES OF PRECONDITIONED SUBSTRATES

MUTANT STRAINS OF THE GENUS CLOSTRIDIUM BEIJERINCKII

Prodn. of food proteins by fermentation - using Trichoderma album and sugar contg. substrates

Device of antennas directed for broad wavebands

Device of antennas directed for broad wavebands

Process and device for a unit for biophysical treatment with resonance reagents, intended to accelerate and optimise processes for the fermentation of cellulolignins.

COMPOSE OF the 2-MONO ACID TYPE OR POLY-CHLOROMETHYL-DITHIO-ACETIQUE AND ITS APPLICATION LIKE ADDITIVE TO FOOD OF the RUMINANTS

PROCESS FOR ENZYMATIC HYDROLYSIS FROM A MIXTURE OF DIFFERENT POROSITES OF PRECONDITIONED SUBSTRATES

PROCEEDED OF DECRISTALLISATION OF CELLULOSE WITH RECYCLING OF THE PHOSPHORIC ACID USES

Saccharification of lignocellulosic material - involves pretreatment with delignifying solvent comprising an amine, pref. ethanolamine, and enzymatic hydrolysis

METHOD FOR MANUFACTURING CELLULOSE SHEET AND CELLULOSE SHEET MANUFACTURED BY METHOD

The present invention relates to a method for manufacturing a cellulose sheet and to a cellulose sheet manufactured by the method, wherein the method for manufacturing the cellulose sheet comprises the following steps: preparing a fixed culture medium for a microbial cellulose production strain; forming a culture medium containing space with four surfaces closed and interconnected, injecting the fixed culture medium for the microbial cellulose production strain into a vertical culture plate which can adjust the interval of two facing walls among the four surfaces depending on the thickness of the cellulose sheet and culturing the culture medium for the microbial cellulose production strain; and separating the cellulose sheet which completed culture from the vertical culture plate, so that the cellulose sheet of uniform thickness can be mass-manufactured in a short time. COPYRIGHT KIPO 2018 ...

pH CONTROLLED YEAST PROPAGATION

BACTERIAL HOST STRAIN COMPRISING A MUTANT SPR GENE AND A WILD-TYPE TSP GENE

DELIGNIFYING PREPARATION EXHIBITING ALPHA-L-ARABINOFURANOSIDASE ACTIVITY PRODUCTION AND APPLICATION THEREOF

PRODUCTION OF ETHANOL FROM LIGNOCELLULOSIC BIOMASS

Described herein are methods for converting lignocellulosic biomass to ethanol, comprising the step of contacting the lignocellulosic biomass with a mixture for a period of time at an initial temperature and an initial pH, wherein the mixture comprises a first microorganism and a second microorganism, thereby producing an amount of ethanol. The first microorganism or the second microorganism may be a thermophilic or mesophilic microorganism. The first microorganism may be a native cellulolytic microorganism or a native xylanolytic microorganism; and the second microorganism may be a genetically engineered xylanolytic microorganism or a genetically engineered cellulolytic microorganism. The microorganisms may be Clostridium thermocellum or Thermoanaerobacterium saccharolyticum, or any number of a wide variety of others.

LIGNIN ENZYME STABILISER

Disclosed is an enzyme stabiliser containing lignin derivatives produced by reacting lignin and a hydrophilic compound, and a lignocellulose biomass saccharification method using the enzyme stabiliser. The enzyme stabiliser increases the activity of diastatic enzymes, and also prevents the non-specific adsorption thereof into a substrate, enabling efficient saccharification of cellulose-type biomass thereby.

CELLULOSE-ACTIVE MICROORGANISMS

Microorganisms that exhibit activity on cellulose and/or cellulose-containing materials are provided. Methods of using such microorganisms and/or agents produced by such microorganisms and screening methods for identifying such microorganisms are also provided.

PRODUCTION OF FERMENTIVE END PRODUCTS FROM CLOSTRIDIUM SP.

In one aspect, methods to enhance the production of ethanol and other fermentive end products from a wide variety of feedstocks by Clostridium microorganisms, such as Clostridium phytofermentans are disclosed. A method of improving fermentation performance of Clostridium microorganisms, such as Clostridium phytofermentans through the use of a fed-batch strategy is described, as well as methods of producing fermentive end products, such as alcohols and/or chemicals by fermenting Clostridium microorganisms, such as Clostridium phytofermentans in the presence of fatty acid-containing compounds and/or at reduced pH.

Enzymatic Hydrolysis Of Pretreated Lignocellulose-Containing Material With Cationic Polysaccharides

A method for producing a fermentation product from a lignocellulose-containing material comprises pre-treating the lignocellulose-containing material; introducing a cationic polysaccharide to the pre-treated lignocellulose-containing material; exposing the pre-treated lignocellulose-containing material to an effective amount of a first hydrolyzing enzyme; and fermenting with a fermenting organism to produce a fermentation product. The cationic polysaccharide may be a cationic starch.

COMPOSITIONS FOR SACCHARIFICATION OF CELLULOSIC MATERIAL

The present invention relates to enzyme compositions for high temperature saccharification of cellulosic material and to uses thereof.

METHOD FOR MULTIPLYING PHYTOBENEFICAL MICROORGANISMS

The invention relates to a method for multiplying a strain of , which includes the preparation of substantially contaminant-free culture media, containing disinfected wood fibers obtained by means of a twin-screw extruder, the amplification of via a series of steps for manufacturing a primary inoculum and then a secondary inoculum from said primary inoculum, and the multiplication of the microorganisms in each step, wherein the multiplication of the propagules reaches 2×10to 10. 1Trichoderma. A method for multiplying a strain of , which comprises:{'i': 'Trichoderma', 'sup': '2', 'the inoculating, with a stock suspension of propagules, of a first sterilized culture medium containing between approximately 25% and approximately 50% by weight of wood fibers, water, nutritive substances and a pH modifier, said fibers having an air content by volume included in the range of from approximately 55% to approximately 90%, said medium being substantially free of fungal contaminants and having a bacterial contaminant content of less than or equal to 10CFU/g;'}{'sup': 4', '5, 'the culturing of the culture medium thus inoculated so as to obtain a primary inoculum in which the multiplication of the propagules reaches 2×10to 10.'}2. The multiplication method as claimed in claim 1 , in which the final concentration of propagules in the primary inoculum is in the range of from 10to 10propagules/g.3. The method as claimed in claim 1 , in which the culturing of the medium comprises an incubation period of at least two weeks.4. The method as claimed in claim 1 , which also comprises:the preparation of a second culture medium containing wood fibers which have been disinfected, said wood fibers having an air content by volume included in the range of from approximately 55% to approximately 90%;the inoculation of said second culture medium with 0.001% to 5% by weight of the primary inoculum;{'sup': 3', '5, 'the culturing of the culture medium thus inoculated so as to obtain a secondary ...

Butanal production using engineered Streptomyces coelicolor

A method of producing butanal by optimizing the growth of Streptomyces using cellulose as food source, overexpressing a key gate enzyme in butyric acid/butyraldehyde production, and knocking out the isobutyryl-CoA synthase gene to shunt the pathway. Optionally, the produced butanal can be isolated and converted into butanol.

Compositions for saccharification of cellulosic material

The present invention relates to enzyme compositions for high temperature saccharification of cellulosic material and to uses thereof.

SYSTEM AND METHOD OF BIOCATALYTIC CONVERSION FOR PRODUCTION OF ALCOHOLS, KETONES, AND ORGANIC ACIDS

Biocatalytic conversion systems and methods of producing and using same that have improved yields are disclosed. The systems and methods involve co-fermentation of sugars and gaseous substrates for alcohol, ketone, and/or organic acid production. The systems and methods may include biocatalytically converting at least one sugar substrate into at least one of alcohol, at least one ketone, and/or at least one organic acid. The systems and methods may further include biocatalytically converting gases that comprise COand Hto at least one alcohol and/or at least one organic acid, thereby adding extra revenue to biorefineries. 1. (canceled)2. A biocatalytic conversion system that utilizes a co-fermentation process for sugars and gases , the biocatalytic conversion system comprising: at least one fermentation medium, wherein the at least one fermentation medium comprises at least one sugar substrate;', {'sub': 2', '2, 'at least one first microorganism, wherein the at least one first microorganism comprises a sugar fermenting species that converts sugars into at least one of acetone, butanol, ethanol, isopropanol, acetic acid, and butyric acid, and wherein a gaseous substrate comprising COand Hgases is produced during the fermentation process; and'}, {'sub': 2', '2, 'at least one second microorganism, wherein the at least one second microorganism comprises a gas fermenting species that converts COand Hgases produced during the fermentation of sugars into at least one of an alcohol and an organic acid.'}], 'at least one reactor comprising3Clostridium, Butyribacterium, Eubacterium, Moorella, Acetobacterium, Enterobacter, Bacillus, Anaerobaculum, Alkalibaculum. The biocatalytic conversion system of claim 2 , wherein each of the first and second microorganisms comprises one or more species of microorganisms claim 2 , and wherein each species is from a genus selected from the group consisting of claim 2 , and combinations thereof.4. The biocatalytic conversion system of claim 3 ...

Methods for the improvement of product yield and production in a microorganism through glycerol recycling

The present invention provides for novel metabolic pathways to reduce or modulate glycerol production and increase product formation. More specifically, the invention provides for a recombinant microorganism comprising one or more native and/or heterologous proteins that function to import glycerol and one or more native and/or heterologous enzymes that function in one or more engineered metabolic pathways to convert a carbohydrate source, such as lignocellulose, to a product, such as ethanol, wherein the one or more native and/or heterologous proteins or enzymes is activated, upregulated, or downregulated. The invention also provides for a recombinant microorganism comprising one or more native or heterologous proteins that function to regulate glycerol synthesis and one or more native and/or heterologous enzymes that function in one or more engineered metabolic pathways to convert a carbohydrate source to ethanol, wherein said one or more native and/or heterologous proteins or enzymes ...

A PROCESS FOR GROWING A MICROBIAL ORGANISM

PROMOTING OF CELLULOSE FERMENTING SPEED

СПОСОБ ПОЛУЧЕНИЯ САХАРНОГО РАСТВОРА

Изобретение относится к пищевой промышленности. Способ включает концентрирование водного полученного из целлюлозы сахарного раствора с помощью нанофильтрационной мембраны и/или обратноосмотической мембраны, в котором указанная концентрация осуществляется после добавления водорастворимого анионного полимера к указанному водному полученному из целлюлозы сахарному раствору, для удаления ингибиторов ферментации на стороне пермеата указанной нанофильтрационной мембраны и/или обратноосмотической мембраны. Водорастворимый анионный полимер включает полимер, выбранный из группы, состоящей из соли фосфатного полимера, фосфатного полимера, соли поликарбоксилатного полимера и поликарбоксилатного полимера. Водорастворимый анионный полимер добавляется к указанному водному полученному из целлюлозы сахарному раствору в концентрации от 0,5 мг/л до 500 мг/л. Средняя молекулярная масса указанного водорастворимого анионного полимера составляет от 200 до 10000. Изобретение обеспечивает производство сахарной ...

СПОСОБ ПОЛУЧЕНИЯ ПО МЕНЬШЕЙ МЕРЕ ОДНОГО ОРГАНИЧЕСКОГО СОЕДИНЕНИЯ

Способ получения по меньшей мере одного органического соединения, имеющего по меньшей мере 3 атома углерода или по меньшей мере 2 атома углерода и по меньшей мере один атом азота посредством ферментации включает следующие стадии: а1) помол зерен злаковых культур в качестве источника крахмала. Полученный размолотый материал содержит по меньшей мере 20 мас.% не содержащих крахмал твердых составляющих источника крахмала. На стадии а2) проводят суспендирование размолотого материала в водной жидкости и ферментативный гидролиз части крахмала в размолотом материале и, при необходимости, последующее осахаривание. Получают жидкость (1), содержащую моносахариды или олигосахариды; и на стадии b) добавляют жидкость (1) в концентрации по меньшей мере 50 мас.% к ферментационной среде, содержащей микроорганизм, продуцирующий органическое соединение, при ферментационных условиях. Изобретение обеспечивает повышение выхода целевого продукта. 16 з.п. ф-лы, 10 табл.

Enzyme-Based Fed-Batch Technique In Liquid Cultures

The present invention is generally in the field of continuous and high-cell-density cultivation in laboratory- or large-scale liquid shaken cultures. More particularly it relates to a method of enzyme-based fed-batch (EnBase) for liquid microbial prokaryotic or eukaryotic cell cultivation having the possibility to manipulate the growth rate of the cultured organisms by a controlled enzymatic release of the growth-limiting substrate-monomer from substrate-polymers or substrate-oligomers.

Mesophilic and Thermophilic Organisms Modified to Produce Acrylate, and Methods of Use Thereof

The present invention provides for novel metabolic pathways leading to acrylate formation in a consolidated bio-processing system (CBP) where lignocellulosic biomass is efficiently converted to acrylate. In one such metabolic pathway, pyruvate is converted to lactate, which is converted to lactoyol-CoA, which is converted to acryloyl-CoA, and which is finally converted to acrylate. In another such metabolic pathway, pyruvate is converted to L-α-alanine, which is converted to L-aspartate, which is converted to β-alanine, which is converted to β-alanyl-CoA, which is converted to acryloyl-CoA, and which is finally converted to acrylate. In yet another metabolic pathway, pyruvate is converted to lactate, and then lactate is converted directly to acrylate. In certain aspects, the invention provides for heterologous expression of one or more enzymes in a mesophilic or thermophilic organism, such as Thermoanaerobacterium saccharolyticum or Clostridium thermocellutn , where the one or more enzymes functions within a novel metabolic pathway as described above to convert pyruvate to acrylate via lactate, or via β alanine and acryloyl-CoA.

Method for fermentation culture in medium containing xylose

The xylose-metabolizing ability and particularly the xylose incorporation rate, of yeast to which xylose-metabolizing ability has been imparted are significantly improved. The method according to the present invention comprises the steps of: culturing yeast having xylose-metabolizing ability in a xylose-containing medium in which the concentration of at least one amino acid selected from the group consisting of asparagine (Asn), serine (Ser), tyrosine (Tyr), threonine (Thr), and histidine (His) is increased; and recovering alcohol from the medium.

Sugar mixtures and methods for production and use thereof

A sugar mixture comprising: monosaccharides; oligosaccharides in a ratio ≧0.06 to total saccharides; disaccharides in a ratio to total saccharides ≧0.05; pentose in a ratio to total saccharides ≧0.05; at least one alpha-bonded di-glucose; and at least one beta-bonded di-glucose. Also disclosed are methods to make and/or use such mixtures.

Substrate-selective co-fermentation process

Biological method for conversion of a sugar-containing organic material into a desired biochemical product. Use of a plurality of substrate-selective cells allows different sugars in a complex mixture to be consumed concurrently and independently. The method can be readily extended to remove inhibitory compounds from hydrolysate.

Microorganisms Having Enhanced Tolerance To Inhibitors and Stress

The present invention provides genetically modified strains of microorganisms that display enhanced tolerance to stress and/or inhibitors such as sodium acetate and vanillin. The enhanced tolerance can be achieved by increasing the expression of a protein of the Sm-like superfamily such as a bacterial Hfq protein and a fungal Sm or Lsm protein. Further, the present invention provides methods of producing alcohol from biomass materials by using the genetically modified microorganisms of the present invention.

Culture substrate comprising cellulose gel, solid medium using same, and cellulase activity assay method using medium

A cellulose gel medium having good visibility of microbial colonies, a cellulose gel culture substrate for manufacturing the cellulose gel medium, a method for manufacturing the cellulose gel culture substrate, a method for screening cellulase-producing microorganisms or cellulase activity with greater efficiency and rapidity, and a culture substrate, which includes a cellulose gel containing cellulose and water as medium-solidifying components, the cellulose has the viscosity of 12 to 35 mPa·S as measured at 26° C. with a solution prepared by dissolving the cellulose at a concentration of 2.5 mg/mL in dimethylacetamide containing 8% (W/V) lithium chloride.

Liquefaction biomass processing with heat recovery

Described are processes that include the non-enzymatic, hydrolytic liquefaction of lignocellulosic biomass to form digest slurries and heat recovery from such digest slurries. Due to enhanced flow properties of the digest slurries such heat recovery can be efficiently conducted in spiral, plate and frame or other heat exchanger designs, with the recovered heat going to unit operations of the process such as heating incoming pretreatment media for the liquefaction. Processes can also involve additional hydrolytic digestion of some or all of the initial slurry components with enzyme and/or additional heat recovery from the initial slurry by direct contact heat exchange in which a portion of the digest slurry liquids is flashed to vapor and that vapor is condensed onto incoming lignocellulosic biomass to the process. Processes as described can be integrated into ethanol manufacture by fermentation of sugars from the digested compositions.

Plant derived cell culture material

The present invention relates material that is useful in culturing and transferring cells as well as delivering cells. The material comprises plant derived cellulose nanofibers or derivatives thereof, wherein the cellulose nanofibers are in a form of a hydrogel or membrane. The invention also provides methods for producing these materials and compositions and uses thereof.

BACTERIAL CULTURE MEDIA AND METHODS FOR THEIR PREPARATION AND USE

Provided herein are methods and compositions for bacterial cellulose production. In some embodiments, the methods and compositions involve or are made from distiller's grain. 1. A method of making culture medium for bacteria , the method comprising:providing distiller's grain from an alcohol fermentation; andmixing the distiller's grain with at least one hydrolytic catalyst, wherein a hydrolysate is formed from the distiller's grain by the hydrolytic catalyst, thereby making a culture medium.2. The method of claim 1 , further comprising collecting the hydrolysate from the mixture.3. The method of claim 2 , further comprising detoxifying the hydrolysate.4. The method of claim 1 , further comprising providing at least one of a carbon source claim 1 , a nitrogen source claim 1 , a salt source claim 1 , and a trace element source.5. The method of claim 1 , wherein the hydrolytic catalyst comprises an acid or an enzyme.6. The method of claim 1 , wherein a ratio of solids to liquids in the mixing of the distiller's grain with the at least one hydrolytic catalyst is about 1:5 (w/v) to about 1:30 (w/v).7. The method of claim 6 , wherein the distiller's grain is mixed with about 0.3% to about 0.7% w/v acid.8. The method of claim 7 , wherein the acid is mixed with the distiller's grain at about 25 to about 200 degrees Celsius for about 30 to about 80 minutes.9. (canceled)10. The method of claim 6 , wherein the distiller's grain is mixed with about 1 to about 700 U of at least one enzyme.11. The method of claim 10 , wherein the enzyme is mixed with the distiller's grain at about 25 to about 90 degrees Celsius for about 0.5 to about 48 hours.12. The method of claim 11 , wherein the enzyme is at least one of cellulase claim 11 , hemicellulase claim 11 , xylanase claim 11 , protease claim 11 , lipase claim 11 , amylase claim 11 , glucan glucohydrolase claim 11 , or glucoamylase.13. (canceled)14. (canceled)15. The method of claim 1 , wherein the distiller's grain is at least one ...

XYLR Mutant For Improved Xylose Utilization Or Improved Co-Utilization Of Glucose And Xylose

The disclosure relates to mutant gene(s) that confer upon microorganisms that express them an improved capacity to utilize xylose and improved capacity to co-utilize glucose and xylose thereby resulting in improved growth of the microorganism. Further encompassed are methods of producing fatty acids and fatty acid derivatives from cellulosic biomass, xylose, and/or a glucose/xylose mix by employing the host cells expressing the engineered XylR variants and compositions of biologically produced fatty acids and fatty acid derivatives. 1. A XylR protein variant , wherein the XylR protein variant has at least one mutation at a position selected from positions 83 , 88 , 89 , 112 , 120 , 141 , 145 , 146 , 147 , 150 , 154 , 155 , 247 , 270 , 280 , 286 , 289 , 295 , 305 , 306 , 313 , 333 , 336 , 337 , 351 , 364 , 365 , 372 , and 382 of SEQ ID NO: 1.2. A recombinant host cell comprising the XylR protein variant of .3. A method for increasing xylose utilization in a recombinant host cell claim 2 , the method comprising culturing in a culture medium comprising xylose claim 2 , the recombinant host cell of claim 2 , wherein expression of the XylR protein variant confers improved xylose utilization of the recombinant host cell in comparison to the xylose utilization of a host cell expressing SEQ ID NO: 1 when the cells are cultured in the presence of xylose.4. The method of claim 3 , wherein the method is used for preparing a fatty acid derivative claim 3 , the method comprising culturing in a culture medium comprising xylose claim 3 , a recombinant host cell which further comprises at least one heterologous fatty acid derivative biosynthetic enzyme.5. The method of claim 4 , wherein the fatty acid derivative is: a fatty acid ester and wherein at least one heterologous fatty acid derivative biosynthetic enzyme has ester synthase activity claim 4 , and optionally wherein at least one heterologous fatty acid derivative biosynthetic enzyme is a thioesterase; a ω-hydroxy fatty acid ...

PELLETS USED IN CELL CULTURE AND METHODS OF MAKING THEREOF

The present invention is directed generally to dry cell culture media or feeds in pellet formats which can be reconstituted into liquid media for culturing cells in vitro. Each pellet composition may comprise the same or a different composition; for example, different vitamins, amino acids, buffers, trace salts, pH, iron chelators, etc. The invention also relates to methods of making dry cell culture media by altering ratios of different pellet compositions, or, methods of making modular dry cell culture media, or customizing media formulations for growing a cell type using pellets. The media pellets may be easier to handle either before reconstitution, during shipping and handling; and/or during reconstitution. Media pellets may be used in any container like bags including sterile, single use bags for preparing media formulations. The invention also relates to kits and culture systems using media pellets. 126.-. (canceled)27. A pelletized cell culture medium , feed , supplement or additive comprising one or more amino acids , one or more binders , one or more vitamins , one or more salts , and one or more trace components , wherein the amino acids are 25 to 40 percent , the binders are 20 to 65 percent , the vitamins are 1 to 5 percent , the salts are 2 to 10 percent , and the trace components are 0.01 to 0.05 percent.28. (canceled)29. The pelletized cell culture medium claim 27 , feed claim 27 , supplement or additive of claim 27 , wherein the vitamins are selected from one or more of vitamin B12 claim 27 , biotin claim 27 , choline claim 27 , folic acid claim 27 , niacinamide claim 27 , pyridoxine claim 27 , riboflavin claim 27 , thiamine claim 27 , ascorbic acid claim 27 , and para-aminobenzoic acid (PABA).30. (canceled)31. The pelletized cell culture medium claim 27 , feed claim 27 , supplement or additive of claim 27 , wherein the salts are selected from one or more of buffer salts claim 27 , iron claim 27 , zinc claim 27 , calcium claim 27 , copper claim 27 , ...

SUGAR MIXTURES AND METHODS FOR PRODUCTION AND USE THEREOF

A sugar mixture comprising: monosaccharides; oligosaccharides in a ratio≥0.06 to total saccharides; disaccharides in a ratio to total saccharides≥0.05; pentose in a ratio to total saccharides≥0.05; at least one alpha-bonded di-glucose; and at least one beta-bonded di-glucose. Also disclosed are methods to make and/or use such mixtures. 161-. (canceled)62. A method comprising:(a) hydrolyzing a lignocellulosic material in a medium containing an acid to form a hydrolyzate; monosaccharides;', 'a ratio of higher oligosaccharides to total saccharides of ≤0.2 weight/weight;', 'a ratio of pentose to total saccharides of ≥0.05 weight/weight;', 'at least one alpha-bonded di-glucose;', 'at least one beta-bonded di-glucose; and', 'a ratio of acid to total saccharides of ≤0.03 weight/weight., '(b) de-acidifying the hydrolyzate to form a de-acidified hydrolyzate comprising63. The method of claim 62 , wherein the hydrolyzing is conducted in a counter-current mode of operation.64. The method of claim 62 , wherein the hydrolyzing is conducted at a temperature of less than 50° C.65. The method of claim 62 , wherein the ratio of the acid to total acid and water in the medium is ≥0.37 by weight.66. The method of claim 62 , wherein the acid is HCl.67. The method of claim 62 , wherein the de-acidifying is conducted at a temperature of less than 80° C.68. The method of claim 62 , wherein the de-acidifying comprises:(i) extracting the hydrolyzate with a first extractant comprising an S1 solvent to form an acid-carrying first extract and an acid-depleted sugar solution, and (ii-a) chromatographic separation, and', '(ii-b) extraction with a second extractant comprising the S1 solvent and an S2 solvent;, '(ii) removing residual acid in the acid-depleted sugar solution by at least one of{'sup': 1/2', '1/2, "wherein the S1 solvent is an organic solvent with a water solubility of less than 15% and is characterized by one or more of a polarity related component of Hoy's cohesion parameter (delta- ...

Compositions for saccharification of cellulosic material

The present invention relates to enzyme compositions for high temperature saccharification of cellulosic material and to uses thereof. 124-. (canceled)25. An isolated recombinant host cell encoding an enzyme composition comprising:(I) a polypeptide having cellobiohydrolase I activity selected from the group consisting of: (a) a polypeptide comprising an amino acid sequence having at least 95% sequence identity to the mature polypeptide of SEQ ID NO: 158; (b) a polypeptide encoded by a polynucleotide that hybridizes under at least high stringency conditions with (i) the mature polypeptide coding sequence of SEQ ID NO: 157, (ii) the genomic DNA sequence of the mature polypeptide coding sequence of SEQ ID NO: 157, or (iii) the full-length complement of (i) or (ii), wherein high stringency conditions are defined as prehybridization and hybridization at 42° C. in 5×SSPE, 0.3% SDS, 200 micrograms/ml sheared and denatured salmon sperm DNA, and 50% formamide and washing three times each for 15 minutes using 2×SSC, 0.2% SDS at 65° C.; (c) a polypeptide encoded by a polynucleotide comprising a nucleotide sequence having at least 95% sequence identity to the mature polypeptide coding sequence of SEQ ID NO: 157; and (d) a polypeptide comprising the mature polypeptide of SEQ ID NO: 158;(II) a polypeptide having cellobiohydrolase II activity selected from the group consisting of: (a) a polypeptide comprising an amino acid sequence having at least 95% sequence identity to the mature polypeptide of SEQ ID NO: 18; (b) a polypeptide encoded by a polynucleotide that hybridizes under at least high stringency conditions with (i) the mature polypeptide coding sequence of SEQ ID NO: 17, (ii) the cDNA sequence of the mature polypeptide coding sequence of SEQ ID NO: 17, or (iii) the full-length complement of (i) or (ii), wherein high stringency conditions are defined as prehybridization and hybridization at 42° C. in 5×SSPE, 0.3% SDS, 200 micrograms/mi sheared and denatured salmon sperm DNA ...

Polypeptides Having Glucuronyl Esterase Activity and Polynucleotides Encoding Same

The present invention relates to isolated polypeptides having glucuronyl esterase activity, catalytic domains and polynucleotides encoding the polypeptides or catalytic domains. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides or catalytic domains.

Method for preparing mutant escherichia coli capable of simultaneously utilizing glucose and xylose

The present invention relates to a method for preparing a mutant E. coli strain, capable of simultaneously using glucose and xylose, by genetic engineering and evolutionary adaptation; the mutant E. coli prepared using the same; and a method for producing biofuels, biologically active ingredients, medicinal materials or base chemicals for the chemical industry using the mutant E. coli . Being capable of simultaneously using glucose and xylose, in contrast to wild-type E. coli , the mutant E. coli can be effectively applied to the enzymatic saccharification process of producing biofuels from a biomass.

RECOMBINANT HOST CELLS AND MEDIA FOR ETHANOL PRODUCTION

Disclosed are recombinant host cells suitable for degrading an oligosaccharide that have been optimized for growth and production of high yields of ethanol, and methods of making and using these cells. The invention further provides minimal media comprising urea-like compounds for economical production of ethanol by recombinant microorganisms. Recombinant host cells in accordance with the invention are modified by gene mutation to eliminate genes responsible for the production of unwanted products other than ethanol, thereby increasing the yield of ethanol produced from the oligosaccharides, relative to unmutated parent strains. The new and improved strains of recombinant bacteria are capable of superior ethanol productivity and yield when grown under conditions suitable for fermentation in minimal growth media containing inexpensive reagents. Systems optimized for ethanol production combine a selected optimized minimal medium with a recombinant host cell optimized for use in the selected medium. Preferred systems are suitable for efficient ethanol production by simultaneous saccharification and fermentation (SSF) using lignocellulose as an oligosaccharide source. The invention also provides novel isolated polynucleotide sequences, polypeptide sequences, vectors and antibodies. 165-. (canceled)66. A minimal medium that supports growth and ethanol production by a recombinant host cell suitable for degrading a saccharide , comprising:a defined nitrogen source;a complex nitrogen source;a source of phosphate; anda source of magnesium.67. The minimal medium according to claim 66 , wherein the defined nitrogen source is a urea-like compound.68. The minimal medium according to claim 67 , wherein the defined nitrogen source is urea.69. The minimal medium according to claim 66 , wherein the complex nitrogen source is selected from the group consisting of corn steep liquor (CSL) claim 66 , yeast autolysate and/or extract claim 66 , corn processing by-product claim 66 , soy ...

PROCESS FOR PROPAGATING A YEAST CAPABLE TO FERMENT GLUCOSE AND XYLOSE

It is disclosed a process for propagating a yeast capable to ferment glucose and xylose of a lignocellulosic feedstock hydrolyzate, said process comprising propagating the yeast over at least two propagation cycles. The first propagation cycle comprises the steps of: contacting the yeast at a starting yeast density with a first cultivation medium comprising a first portion of the lignocellulosic feedstock hydrolyzate; and allowing the yeast to propagate to create a first populated broth comprising water and a first propagated yeast, wherein at least 50% of the glucose and less than 20% of the xylose in the first cultivation medium are consumed in the first propagation cycle. The second cycle comprises the steps of: separating the first populated broth in at least a first removed portion and a first residual portion, wherein both the first residual portion and the first removed portion comprise some of the first propagated yeast; contacting the first residual portion with a second cultivation medium comprising a second portion of the lignocellulosic feedstock hydrolyzate; and allowing the yeast to propagate to create a second populated broth comprising water and a second propagated yeast, wherein at least 50% of the glucose and less than 20% of the xylose in the second cultivation medium are consumed in the second propagation cycle. 119-. (canceled)20. A process for propagating a yeast capable of fermenting glucose and xylose of a lignocellulosic feedstock hydrolyzate , said process comprising:propagating the yeast over at least a first propagation cycle and a second propagation cycle,{'sub': 'n', 'claim-text': a. contacting the yeast at a starting yeast density with a first cultivation medium comprising a first portion of the lignocellulosic feedstock hydrolyzate,', 'b. allowing the yeast to propagate to create a first populated broth comprising water and a first propagated yeast, wherein at least 50% of the glucose and less than 20% of the xylose in the first ...

METHOD OF PRODUCING BIOPRODUCTS

Methods for production of a bioproduct with a microorganism and selective extraction of bioproducts from a fermentation broth. The methods may include mixing a carbon source, a nitrogen source, and an extractant-depleted raffinate to form a fermentation medium, and fermenting the medium with a microorganism to form a fermentation broth having at least one bioproduct. The bioproduct may be extracted from the fermentation broth with an extractant comprising an oxygenated organic compound and a hydrocarbon to form an extract and a raffinate, and the extract may be further separated from the raffinate. The bioproduct may then be separated from the extract, and the extractant may be separated from the raffinate to regenerate the ex tract-depleted raffinate. 148.-. (canceled)49. A method for producing at least one bioproduct comprising:(i) mixing a carbon source and a nitrogen source to form a fermentation medium;(ii) fermenting said medium with a microorganism to form a fermentation broth containing a bioproduct;(iii) extracting at least a fraction of said fermentation broth with an extractant comprising an oxygenated organic compound and a hydrocarbon to form an extract and a raffinate, wherein both extract and raffinate comprise said oxygenated organic compound, said bioproduct, and water;(iv) separating said extract from said raffinate;(v) separating at least a fraction of the bioproduct from said extract; wherein said separating comprises separating at least a fraction of said oxygenated organic compound from said extract to form an extractant-depleted bioproduct solution,(vi) separating at least a fraction of said oxygenated organic compound from said raffinate to generate an extractant-depleted raffinate; and(vii) liquefying at least a fraction of the separated oxygenated organic compound with a refrigerant in a refrigerant circuit, wherein the refrigerant in the refrigerant circuit is selected from the group consisting of R-11, R-12, R-13, R-14, R-21, R-22, R-23, ...

SYSTEM FOR MANAGEMENT OF YEAST TO FACILITATE THE PRODUCTION OF ETHANOL

A system and method for managing an ethanologen for use in biorefinery is disclosed. The method for propagating ethanologen for use in the production of a fermentation product from biomass comprises the steps of providing a medium for propagation of ethanologen and supplying a first cell mass of ethanologen to the medium. A first cell mass of ethanologen is propagated into a larger second cell mass of ethanologen. 1. A method of propagating ethanologen for use in the production of a fermentation product from biomass comprising the steps of:providing a medium for propagation of ethanologen;supplying a first cell mass of ethanologen to the medium;supplying xylose to the medium as a carbon source for cell mass growth of the ethanologen;maintaining the medium comprising the first cell mass of ethanologen at a pH of between about 3.5 and 6.5 and at a temperature of between about 26 and about 37 degrees Celsius so that the first cell mass of ethanologen is propagated into a second cell mass of ethanologen;aerating the medium so that the first cell mass of ethanologen is propagated into a second cell mass of ethanologen;and wherein, the second cell mass of ethanologen is at least 200 times larger than the first cell mass of ethanologen.2. The method of wherein xylose is the sole source of carbon supplied to the medium.3. (canceled)4. (canceled)5. The method of wherein the biomass comprises lignocellulosic material.6. (canceled)7. The method of wherein the xylose supplied to the medium is obtained from the lignocellulosic material.8. (canceled)9. The method of wherein the ethanologen comprises yeast cells capable of fermenting xylose into ethanol.1018.-. (canceled)19. The method of wherein xylose is obtained as a component of the biomass and wherein xylose is supplied to the medium comprises at a rate of at least 0.12 grams of xylose per minute.20. (canceled)21. (canceled)22. The method of wherein xylose is supplied to the medium at a concentration of at least 1 percent by ...

Polypeptides Having Glucuronyl Esterase Activity and Polynucleotides Encoding Same

The present invention relates to isolated polypeptides having glucuronyl esterase activity, catalytic domains and polynucleotides encoding the polypeptides or catalytic domains. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides or catalytic domains.

PROCESS FOR ENZYMATIC HYDROLYSIS FROM A MIXTURE OF PRE-TREATED SUBSTRATES OF DIFFERENT POROSITIES

The present invention relates to a process for enzymatic hydrolysis in which, under agitation, pre-treated lignocellulosic substrates are brought into contact with water and with enzymes such that the mixture has a content of dry matter of between 12 and 35% by weight, said process being characterised in that a mixture is used of at least two pre-treated lignocellulosic substrates with different porosities, at least one of the substrates being a substrate said to be of low porosity having a porosity of less than 60% of the volume and the other substrate a substrate said to be of high porosity having a porosity greater than or equal to 60% of the volume, and said substrate of low porosity being present in a quantity of at least 30% by weight in relation to the total weight of said mixture. 1. Process for enzymatic hydrolysis in which , under agitation , pre-treated lignocellulosic substrates are brought into contact with water and with enzymes such that the mixture has a content of dry matter of between 12 and 35% by weight , said process being characterised in that a mixture is used of at least two pre-treated lignocellulosic substrates with different porosities , at least one of the substrates being a substrate said to be of low porosity having a porosity of less than 60% of the volume and the other substrate a substrate said to be of high porosity having a porosity greater than or equal to 60% of the volume , and said substrate of low porosity being present in a quantity of at least 30% by weight in relation to the total weight of said mixture.2. Process according to in which the substrate of low porosity is present in a quantity of between 30 and 50% by weight in relation to the total weight of said mixture.3. Process according to in which the substrate of low porosity is present in a quantity of between 40 and 50% by weight in relation to the total weight of said mixture.4. Process according to in which the substrate said to be of low porosity has a porosity of ...

PROCESS FOR PRODUCING LACTIC ACID OR ITS SALTS FROM FERMENTATION USING THERMOTOLERANCE BACILLUS BACTERIA

This invention relates to a process for producing lactic acid or its salts that can be performed easily, reduce complicated steps, and provide high lactic acid yield and high productivity, wherein said process comprising the following steps: (a) cultivating thermotolerance genus bacteria to obtain a seed culture; (b) increasing cell number of bacteria by inoculating the seed culture obtained from step (a) into a fermenter containing an initial carbon source under an aerobic condition; (c) fermenting the seed culture obtained from step (b) in the fermenter under a microaerobic condition to obtain lactic acid or its salts; wherein the step (b) comprising at least one addition of the carbon source under any one of the following conditions, which are independent to each other, to increase a concentration of the carbon source: —when the concentration of carbon source in the fermenter reduces to 50% or less comparing to the initial concentration. 1. A process for producing lactic acid or its salts , comprising the steps of:{'i': 'Bacillus', '(a) cultivating thermotolerance genus bacteria to obtain a seed culture;'}{'i': 'Bacillus', '(b) increasing the cell number of the thermotolerance genus bacteria by inoculating the seed culture obtained from step (a) into a fermenter containing an initial carbon source under an aerobic condition;'}(c) fermenting the seed culture obtained from step (b) in the fermenter under a microaerobic condition to obtain lactic acid or its salts; when the concentration of the carbon source in the fermenter reduces to 50% or less compared to the initial concentration;', 'when the (b) is carried out for a time of at least one third;', 'when an optical density (OD) of bacteria cells in the fermenter increases by at least 10 times., 'wherein step (b) further comprises at least one addition of the carbon source under any one of the following conditions, which are independent of each other, to increase the concentration of the carbon source2. The ...

Oleaginous Yeast and its application

Disclosed is oleaginous yeast and its application, belonging to the technical field of microorganisms. The oleaginous yeast provided by the disclosure has been deposited in China Center for Type Culture Collection on May 21, 2020, with an accession number of CCTCC NO: M 2020139. The oleaginous yeast provided by the disclosure can use a hydrolysate of cheap lignocellulose biomass as the substrate. Xylose and glucose in the hydrolysate are simultaneously utilized to produce oil, so the sugar utilization efficiency and production intensity are enhanced. After 8 days of fed-batch fermentation, the oil yield can reach 31.33 g/L, and the oil content can reach 60.83%. The waste resources can be reutilized, and the production cost is reduced, so the oleaginous yeast has wide application prospects. 1Trichosporon dermatis. A method for producing oil by simultaneously utilizing xylose and glucose , comprising adding an oleaginous yeast or a culture thereof to a system containing xylose and glucose; wherein the oleaginous yeast is classified and named as which has been deposited in China Center for Type Culture Collection on May 21 , 2020 , with an accession number of CCTCC NO: M 2020139.2. The method according to claim 1 , wherein the oleaginous yeast is fermented at 22-28° C. for 7-10 days.3. The method according to claim 1 , wherein the system is a fermentation system to produce oil by fermentation.4. The method according to claim 3 , wherein the fermentation system contains a carbon source claim 3 , and the carbon source is xylose and glucose.5. The method according to claim 3 , wherein the fermentation system contains a carbon source claim 3 , and the carbon source is from a hydrolyzed mixed sugar solution of a lignocellulose biomass raw material.6. The method according to claim 3 , comprising adding a seed solution of the oleaginous yeast with an ODof 6-8 to the fermentation system at an addition amount of 5%-10%.7. The method according to claim 3 , wherein a total sugar ...

REGULATION OF THE RAS/CAMP/PKA SIGNALING PATHWAY IN YEASTS

The present disclosure relates to the modulation in the RAS/cAMP/PKA signaling pathway for maintaining the propagation efficiency and increasing fermentation efficiency of yeast cells. The present disclosure provides yeast cells having or engineered to exhibit a modulation in signaling in a RAS/cAMP/PKA pathway, depending on conditions. For example the yeast cells can be selected or genetically modified to express a mutated Ras1 protein, a mutated Ras2 protein, a mutated Ira1 protein and/or a mutated Ira2 protein, optionally in combination with specific promoters. Also provided herewith are methods for propagating the yeast cells as well as using the yeast cells to generate a fermented product (such as ethanol). 24.-. (canceled)5. The method of claim 1 , wherein the first yeast cell is or has been selected as being capable of expressing a mutated Ras2 protein having increased activity when compared to a wild-type Ras2 protein.6. The method of claim 5 , wherein the mutated Ras2 protein includes at least one amino acid residue variation when compared to the wild-type Ras2 protein.7. The method of claim 6 , wherein the mutated Ras2 protein has the amino acid sequence of SEQ ID NO: 2 or is encoded by a nucleic acid molecule having the sequence of SEQ ID NO: 3.811.-. (canceled)12. The method of claim 1 , wherein the heterologous nucleic acid molecule comprises a first nucleic acid molecule coding for a mutated Ras2 protein having increased activity when compared to a wild-type Ras2 protein.13. The method of claim 12 , wherein the mutated Ras2 protein includes at least one amino acid residue variation when compared to the wild-type Ras2 protein.14. The method of claim 13 , wherein the mutated Ras2 protein has the amino acid sequence of SEQ ID NO: 2 or is encoded by a nucleic acid molecule having the sequence of SEQ ID NO: 3.1517.-. (canceled)18. The method of claim 12 , wherein the heterologous nucleic acid molecule further comprises a promoter operatively linked to the ...

Preparation of Microbial Cellulose

The properties of microbial pellicles are tuned by adjusting the physical culture conditions. A culture of can be grown in a liquid growth media having a surface exposed to air so that a basal pellicle of microbial cellulose forms on the surface. Feeding the culture by adding additional liquid growth media at the surface, thereby submerging the basal pellicle; and then allowing the culture to grow again forms a second pellicle of microbial cellulose. 1. A method of preparing microbial cellulose comprising:{'i': 'Gluconacetobacter xylinus', 'growing a culture of in a liquid growth media having a surface exposed to air and allowing a basal pellicle of microbial cellulose to form at the surface; then'}feeding the culture by adding additional liquid growth media to the surface, thereby submerging the basal pellicle; and thenallowing the culture to grow, thereby forming a second pellicle of microbial cellulose,wherein the second pellicle has a thickness of about 10 μm or less as measured when the second pellicle is dried.2. The method of claim 1 , further comprising an additional feeding to form a third pellicle having a thickness of about 10 μm or less as measured when the third pellicle is dried.3. The method of claim 1 , further comprising treating the second pellicle with a solution of NaOH and then washing the second pellicle with water before drying the second pellicle.4. The method of claim 1 , wherein the second pellicle comprises microfibrils of bacterial cellulose having lengths of from 1 μm to 10 μm.5. The method of claim 1 , wherein the second pellicle of microbial cellulose is composed primarily of nanocellulose.6. A microbial cellulose pellicle comprising:microfibrils of bacterial cellulose having lengths of from 1 μm to 10 μm, wherein the pellicle has a thickness of about 10 μm or less as measured when the pellicle is dried.7. A microbial cellulose pellicle of claim 6 , wherein the pellicle has a thickness of about 2 μm or less as measured when the ...

METHODS OF PITCHING YEAST FOR FERMENTATION, AND RELATED METHODS OF FERMENTATION AND SYSTEMS

The present invention relates to methods and systems of pitching yeast to fermentation reactors. More particularly, the present invention involves pitching yeast from one fermentation tank to at least one additional fermentation tank. Advantageously, yeast can be continuously pitched from fermentor to fermentor for as long as practically desirable. 1. A method of fermenting alcohol , the method comprising:providing a first aqueous composition in a first fermentation reactor, wherein the first aqueous composition comprises yeast, water, processed plant material comprising milled grain or lignocellulosic biomass, and one or more enzymes that can break down the processed plant material to yield one or more monosaccharides;fermenting the first aqueous composition in the first fermentation reactor to form a first beer composition comprising alcohol;providing a second aqueous composition in a second fermentation reactor, wherein the second aqueous composition comprises, water, processed plant material comprising milled grain or lignocellulosic biomass, and one or more enzymes that can break down the processed plant material to yield one or more monosaccharides, and a fraction of the first beer composition including yeast and alcohol from the first beer composition;fermenting the second aqueous composition in the second fermentation reactor to form a second beer composition comprising alcohol;recovering alcohol from the first beer composition from the first fermentation reactor; andrecovering alcohol from the second beer composition from the second fermentation reactor.2. The method of claim 1 , wherein adding the yeast to the first fermentation reactor comprises dry-batching yeast to the first fermentation reactor.3. The method of claim 1 , wherein claim 1 , prior to removing the fraction of the first beer composition from the first fermentation reactor claim 1 , at least the fraction of the first aqueous composition is exposed to a temperature in the range from 15° C. to ...

PROCESSING OF BIOMASS MATERIALS

The use of cell matter in fermentation mixtures for producing a product is disclosed. In embodiments, the product comprises carbohydrates, alcohols, or organic acids (e.g., lactic acid or succinic acid), or mixtures thereof. 1. A method of making a product , the method comprising contacting one or more sugars with a fermentation composition comprising lysed cell matter to produce the product.2. The method of claim 1 , wherein the one or more sugars comprise xylose and glucose.3. The method of claim 1 , wherein the one or more sugars are formed by saccharifying a biomass material comprising lignocellulosic material.4. The method of claim 3 , wherein the lignocellulosic material comprises an agricultural product or waste claim 3 , a paper product or waste claim 3 , a forestry product or waste claim 3 , or a general waste.58-. (canceled)9. The method of claim 3 , wherein the lignocellulosic material has been pretreated to reduce its recalcitrance by treating the lignocellulosic material with an electron beam claim 3 , sonication claim 3 , oxidation claim 3 , pyrolysis claim 3 , steam explosion claim 3 , heat treatment claim 3 , chemical treatment claim 3 , mechanical treatment claim 3 , or freeze grinding.1011-. (canceled)12. The method of claim 1 , wherein the one or more sugars are isolated prior to contact with the fermentation composition.13. The method of claim 1 , wherein the lysed cell matter comprises lysed fungal cells.14Coprinus, Myceliophthora, Scytalidium, Penicillium, Aspergillus, Humicola, Fusarium, Thielavia, Acremonium, Chrysosporium, Saccharomyces, Candida, Clavispora, Pichia, YarrowiaTrichoderma.. The method of claim 13 , wherein the fungal cells comprise a species in the genera selected from claim 13 , and15. (canceled)16Trichoderma reesei.. The method of claim 15 , wherein the fungal cells comprise the species17Trichoderma reesei. The method of claim 16 , wherein the comprises any individual strain claim 16 , variant claim 16 , or mutant thereof. ...

USE OF HYDROTHERMALLY TREATED BIOMASS AS PATHOGEN BINDER

The present invention relates to microorganism biomass and a feed or food composition comprising said biomass for use in preventing or reducing the adverse effects of pathogens in animal or human digestive tract. 1. A method for preparing a feed additive , said method comprising the steps of(a) providing an aqueous suspension comprising microbial biomass,(b) subjecting said aqueous suspension comprising said microbial biomass to a temperature of at least 160° C. for is to 360 minutes at a pressure above 5 bar,(c) separating a solid phase containing the microbial biomass,(d) optionally, drying the microbial biomass obtained from step (c).2. The method according to claim 1 , wherein step (b) is conducted under conditions corresponding to a severity factor of at least 2.5 claim 1 , preferably of at least 3.5 claim 1 , most preferably at least 3.9.3. The method according to claim 1 , wherein step (b) is conducted under conditions corresponding to a severity factor in the range of of about 3.5 to about 5.5 such as e.g. about 4 to about 4.9 claim 1 , such as e.g. about 4.1 to about 4.8.4. The method according to claim 1 , wherein step (b) is conducted during a period of e.g. about 5 minutes and up to about 120 minutes claim 1 , such as e.g. about 10 minutes and up to about 60 minutes claim 1 , while the temperature is about 160° C. and up to about 220° C. claim 1 , such as e.g. about 180° C. and up to about 220° C.5. The method according to claim 1 , wherein said aqueous suspension comprising microbial biomass contains cultivation medium comprising lignocellulose hydrolysate.6. The method according to claim 1 , wherein the microbial biomass obtained in step (c) further comprises residues of lignocellulose.7. The method of according to claim 1 , wherein the microbial biomass is obtained from oleaginous microorganisms.8. The method of according to claim 5 , wherein the microbial biomass of step (c) is obtained by subjecting the oleaginous microorganisms to a step of ...

METHOD FOR LOADING OF MICROORGANISMS ON MULTIPHASE BIOMATERIALS

The invention relates to method for loading microorganisms or parts thereof on and/or in pre-synthesized multiphase biomaterials comprising nanocellulose wherein the microorganisms are resuspended in a buffer or a culture medium and loaded into and/or onto the multiphase biomaterial and the use of such a loaded multiphase biomaterial in nutritional, food, pharmaceutical, medical, cosmetic, especially oral, mucosal, dermal and transdermal, ocular, dermatological or female health applications. 1. A method for loading microorganisms or parts thereof on and/or in a pre-synthesized multiphase biomaterial comprising nanocellulose , the method comprising:synthesizing a bacterially synthesized nanocellulose (BNC) multiphase biomaterial,resuspending the microorganisms in a buffer or a culture medium, and mixing the multiphase biomaterial with the microorganisms at 300 rpm or more at a temperature of 37° C. or less, or', 'injecting the microorganisms into the multiphase biomaterial and incubating at a temperature of 37° C. or less, or', 'incubating the multiphase biomaterial in the buffer or culture medium with resuspended microorganisms at a temperature of 37° C. or less for 60 min or less., 'loading the microorganisms into and/or onto the BNC material by either2. The method of claim 1 , wherein the microorganisms are sprayed onto the multiphase biomaterial.3. The method of claim 1 , wherein the microorganisms are loaded as vegetative cells or in a dormant form claim 1 , or as a cell-extract.4. The method of claim 1 , wherein the microorganisms are wet or dry and/or pre-cultured or not pre-cultured.5. The method of claim 1 , wherein the multiphase biomaterial is wet or dried or partially dried or re-swelled in buffer.6. The method of claim 1 , wherein the nanocellulose is derived from a plant claim 1 , algae claim 1 , or a microorganism.7. The method of claim 1 , wherein the bacterially synthesized nanocellulose (BNC) comprises a layered structure.8. The method of claim 1 , ...

EXTREME THERMOPHILIC BACTERIA OF THE GENUS CALDICELLULOSIRUPTOR SUITABLE FOR THE CONVERSION OF CELLULOSIC AND STARCHY BIOMASS

Isolated cellulolytic extreme thermophilic bacterial cells belonging to the genus , mutants thereof, isolated strains, microbial cultures and microbial compositions. The novel bacteria are in particular suitable for the production of fermentation products such as lactic acid from any carbon source, not limited to cellulosic material but especially useful for converting cellulosic biomass like lignocellulosic biomass and/or starch containing biomass. 129-. (canceled)30CaldicellulosiruptorCaldicellulosiruptor, Caldicellulosiruptor, CaldicellulosiruptorCaldicellulosiruptor, Caldicellulosiruptor, CaldicellulosiruptorCaldicellulosiruptor. A method for converting lignocellulosic biomass and/or starch containing biomass to a carboxylic acid comprising the step of contacting the lignocellulosic biomass and/or the starch containing biomass with a microbial culture for a period of time at an initial temperature and an initial pH , thereby producing an amount of a carboxylic acid; wherein the microbial culture comprises an extremely thermophilic bacteria strain of the genus , wherein the strain is selected from the group consisting of sp. BluConL70 having the DSMZ Accession number 33496sp. BluConL60 having the DSMZ Accession number 33252sp. BluCon085 having the DSMZ Accession number 33485 sp. BluCon052 having the DSMZ Accession number 33470sp. BluCon006 having the DSMZ Accession number 33095sp. BluCon014 (DSMZ Accession number 33096) and sp. BluCon016 (DSMZ Accession number 33097) , microorganism derived therefrom , progenies or mutants thereof , wherein the mutants thereof retaining the properties of BluCon0L70 , BluConL60 , BluCon085 , BluCon052 , BluCon006 , BluCon014 and/or BluCon016.3136-. (canceled)37Miscanthus. The method according to claim 30 , wherein the lignocellulosic biomass is selected from the group consisting of grass claim 30 , switch grass claim 30 , cord grass claim 30 , rye grass claim 30 , reed canary grass claim 30 , mixed prairie grass claim 30 , claim ...

Production of malonyl-coa derived products via anaerobic pathways

The present invention provides for novel metabolic pathways to convert biomass and other carbohydrate sources to malonyl-CoA derived products, such as hydrocarbons and other bioproducts, under anaerobic conditions and with the net production of ATP. More specifically, the invention provides for a recombinant microorganism comprising one or more native and/or heterologous enzymes that function in one or more engineered metabolic pathways to achieve conversion of a carbohydrate source to, e.g., long-chain hydrocarbons and hydrocarbon derivatives, wherein the one or more native and/or heterologous enzymes is activated, upregulated, downregulated, or deleted. The invention also provides for processes to convert biomass to malonyl-CoA derived products which comprise contacting a carbohydrate source with a recombinant microorganism of the invention.

Lignocellulosic conversion processes and products

Processes for converting lignocellulose to feedstock and downstream products are disclosed. The processes may include acid treatment of lignocellulose to produce a fermentation feedstock. In various instances, the processes include recovery or recycling of acid, such as recovery of hydrochloric acid from concentrated and/or dilute streams. Downstream products may include acrylic acid-based products such as diapers, paper and paper-based products, ethanol, biofuels such as biodiesel and fuel additives, and detergents.

PELLETS USED IN CELL CULTURE AND METHODS OF MAKING THEREOF

The present invention is directed generally to dry cell culture media or feeds in pellet formats which can be reconstituted into liquid media for culturing cells in vitro. Each pellet composition may comprise the same or a different composition; for example, different vitamins, amino acids, buffers, trace salts, pH, iron chelators, etc. The invention also relates to methods of making dry cell culture media by altering ratios of different pellet compositions, or, methods of making modular dry cell culture media, or customizing media formulations for growing a cell type using pellets. According to the invention, media pellets may be easier to handle either before reconstitution, during shipping and handling; and/or during reconstitution. Media pellets may be used in any container like bags including sterile, single use bags for preparing media formulations. The invention also relates to kits and culture systems using media pellets. 1. A method of making a pelletized cell culture medium , the method comprising:a) subjecting a first dry powder to suspension in an upwardly moving column of a gas in a fluid bed apparatus, and to spinning in a disc rotor;b) introducing a solvent and/or a second dry powder into the fluid bed apparatus at step (a), such that the pellet is formed; andc) drying the pellet,wherein the first dry powder, the second dry powder, or the solvent comprises a binder, an excipient, or both.2. The method of making pelletized cell culture medium of claim 1 , wherein the first dry powder of step (a) is pre-wetted claim 1 , and optionally claim 1 , no solvent is introduced in step (b).3. The method of making pelletized cell culture medium of claim 1 , wherein the first and second dry powders are either the same type of dry powders or different type of dry powders.4. (canceled)5. The method of making pelletized cell culture medium of claim 1 , wherein the first dry powder or the second dry powder is selected from the group consisting of a basal media powder ...

METHODS FOR PROPAGATING MICROORGANISMS FOR FERMENTATION & RELATED METHODS & SYSTEMS

Disclosed are compositions, methods, and systems for propagating microorganisms for fermentation. 1. A method of propagating a microorganism , the method comprising:a) providing a first propagation medium comprising at least a lignocellulosic hydrolysate comprising at least one monosaccharide at a concentration from about 0.1 wt % to 2.0 wt % of the propagation medium, and at least one inhibitor at a concentration of about 1000 ppm or less;b) growing a first cell mass of the microorganism on the propagation medium to form a second cell mass of the microorganism, wherein the second cell mass of microorganism is greater than the first cell mass of the microorganism; andc) using the second cell mass of the microorganism for fermentation to produce a fermentation product.2. The method as in claim 1 , wherein the first cell mass of the microorganism is from about 0.001 wt % to 0.05 wt % of propagation medium.3. The method as in as in claim 1 , wherein the at least one inhibitor comprises furfural claim 1 , 5-hydroxymethylfurfural (HMF) or combinations thereof.4. The method as in claim 1 , wherein providing the first propagation medium comprises:a) forming a lignocellulosic hydrolysate composition comprising at least one inhibitor greater than 1000 ppm; andb) reducing the at least one inhibitor to less than 1000 ppm.5. The method as in claim 4 , wherein the reducing is by dilution or gas stripping or both.6. The method as in as in claim 5 , wherein the gas stripping is by with air claim 5 , nitrogen claim 5 , ozone or combinations thereof.7. The method as in as in claim 1 , wherein the second cell mass of the microorganism comprises 100 to 1000 times the first cell mass of microorganisms.8. The method as in claim 1 , wherein the first propagation medium further comprises a stillage component.9. The method as in claim 8 , wherein the stillage component is thin stillage.10. The method of claim 1 , wherein substantially no ethanol is produced by the first cell mass of the ...

Molding System for Fungal Structures

A molding system for forming an inoculated lignocellulose based medium into a fungal molded shape, the molding system comprising a vessel within which environmental conditions are regulated, the vessel comprising an inoculated lignocellulose based medium capable of supporting growth of saprophytic fungi without any secondary organisms displacing the process through infection a secondary organic material layered near the top and bottom of the inoculated lignocellulose based medium, a hard mold containing the flexible vessel; and a compressive system for applying a primary compressive pressure of at least 10 PSI to the lignocellulose based medium such that it takes on a fungal molded shape. 1. A molding system for forming an inoculated lignocellulose based medium into a fungal molded shape , the molding system comprising:a. a vessel within which environmental conditions are regulated, the vessel comprising an inoculated lignocellulose based medium capable of supporting growth of saprophytic fungi without any secondary organisms displacing the process through infection;b. a secondary organic material layered near the top and bottom of the inoculated lignocellulose based medium;c. a hard mold containing the flexible vessel; andd. a compressive system for applying a primary compressive pressure of at least 10 PSI to the lignocellulose based medium such that it takes on a fungal molded shape.2. The molding system of wherein said flexible vessel environmental conditions are between 55 and 90 degrees Fahrenheit.3. The molding system of wherein said flexible vessel environmental conditions include a hydration level of between 44% and 66%.4. The molding system of wherein said vessel comprises a flexible breathable filter membrane or flexible breathable filter membrane patch allowing gas exchange while preventing the passage of bacteria and microorganisms.5. The molding system of wherein said fungal molded shape comprises an outer surface of mycelium.6. The molding system of ...

CELLS WITH IMPROVED PENTOSE CONVERSION

The invention relates to a cell capable of converting one or more pentose sugar and one or more hexose sugar into fermentation product constitutively expressing one or more heterologous or homologous polypeptide having the amino acid sequence set out in SEQ ID NO: 20, or a variant polypeptide thereof having at least 45% identity to SEQ ID NO 20. In an embodiment the heterologous polypeptide has glyoxalase activity. 1. A cell capable of converting one or more pentose sugar and one or more hexose sugar into fermentation product constitutively expressing one or more heterologous or homologous polypeptide having the amino acid sequence set out in SEQ ID NO: 20 or a variant polypeptide thereof , having at least 45% identity to SEQ ID NO 20.2. A cell according to claim 1 , wherein the heterologous polypeptide has glyoxalase activity claim 1 , optionally comprising glyoxalase I activity.3. An cell capable of converting one or more pentose sugar and or one or more hexose sugar into fermentation product comprising a constitutively expressed heterologous or homologous polynucleotide which comprises:(a) the nucleotide sequence as set out in SEQ ID NO: 27;(b) a nucleotide sequence having at least about 50% sequence identity with the nucleotide sequence of SEQ ID NO: 27;(c) a fragment of a nucleotide sequence as defined in (a), (b) or (c) having at least 100 nucleotides;(d) a sequence which is degenerate as a result of the genetic code to a sequence as defined in any one of (a), (b), or (c);(e) a nucleotide sequence which is the reverse complement of a nucleotide sequence as defined in (a), (b), (c), or (d).4. A cell according to claim 1 , comprising a nucleotide sequence encoding a xylose isomerase.5. A cell according to claim 1 , wherein the cell comprises one or more genetic modifications resulting in:(a) an increase in transport of xylose in the cell;(b) an increase in xylulose kinase activity;(c) an increase in flux through the pentose phosphate pathway;(d) a decrease in ...

Lactic acid bacteria for the production of ethanol from biomass material

Lactic acid bacterial cultures, cell populations and articles of manufacture comprising same are disclosed for generating ethanol from lignocellulse.

Molding System for Fungal Structures

A molding system for forming an inoculated lignocellulose based medium into a fungal molded shape, the molding system comprising a vessel within which environmental conditions are regulated, the vessel comprising an inoculated lignocellulose based medium capable of supporting growth of saprophytic fungi without any secondary organisms displacing the process through infection. a secondary organic material layered near the top and bottom of the inoculated lignocellulose based medium, a hard mold containing the flexible vessel; and a compressive system for applying a primary compressive pressure of at least 10 PSI to the lignocellulose based medium such that it takes on a fungal molded shape. 1. A molding system comprising:a. a first inoculated lignocellulose based medium;b. the first inoculated lignocellulose based medium comprising nitrogen, trace elements, and a buffer to balance the pH of the first inoculated lignocellulose based medium;c. a second, grown inoculated lignocellulose based medium;d. a secondary material layered between said first and second media, the secondary material comprising a cross-grained laminate of at least two layers comprising components set at right angles relative to one another;e. a filter for filtering the inoculated lignocellulose based medium; andf. a compressive system for applying a primary compressive pressure of at least 10 PSI to the lignocellulose based medium and a secondary compressive pressure of between 300-500 psi to the lignocellulose based medium such that at least some water is forced out of the medium, allowing it to absorb an agent and take on a fungal molded shape;g. whereby said second, grown inoculated lignocellulose based medium contains in it a cellulosic or non-cellulosic fabric.2. The molding system of claim 1 , wherein the first inoculated lignocellulose based medium is within a vessel in which environmental conditions are regulated.3. The molding system of wherein the vessel is within a hard mold; and wherein ...

Processing biomass

Feedstocks, obtained at least in part from a plant material that has been modified with respect to its wild type, are processed to produce useful intermediates and products, such as energy, fuels, foods or materials. For example, systems are described that can treat such feedstock materials, e.g., to reduce the recalcitrance of the feedstock, and use the treated feedstock materials to produce an intermediate or product, e.g., by saccharification and/or fermentation.

RECOMBINANT THRAUSTOCHYTRIDS THAT GROW ON XYLOSE, AND COMPOSITIONS, METHODS OF MAKING, AND USES THEREOF

The present invention is directed to recombinant thraustochytrids that grow on xylose and cell cultures comprising the recombinant thraustochytrids as well as methods of producing cell cultures, biomasses, microbial oils, compositions, and biofuels using the recombinant thraustochytrids. 135-. (canceled)36. A thraustochytrid cell comprising a nucleic acid molecule comprising a polynucleotide sequence encoding a polypeptide associated with xylose import , conversion of xylose to xylulose , phosphorylation of xylulose , or a combination thereof , and wherein said polypeptide is bound to the plasma membrane of the thraustochytrid cell.37. The cell of claim 36 , wherein the polypeptide associated with xylose import claim 36 , conversion of xylose to xylulose claim 36 , or phosphorylation of xylulose is selected from the group consisting of: a heterologous xylose transporter claim 36 , a heterologous xylose isomerase claim 36 , a heterologous xylulose kinase claim 36 , a heterologous xylose reductase claim 36 , a heterologous xylitol dehydrogenase claim 36 , and combinations thereof.38. The cell of claim 36 , wherein the wherein the thraustochytrid cell expresses a heterologous xylose transporter claim 36 , a heterologous xylose isomerase claim 36 , and a heterologous xylulose kinase.39. The cell of claim 36 , wherein the thraustochytrid cell expresses a heterologous xylose transporter claim 36 , a heterologous xylulose kinase claim 36 , a heterologous xylose reductase claim 36 , and a heterologous xylitol dehydrogenase.40. The cell of claim 39 , wherein the polynucleotide sequence encoding the polypeptide associated with xylose import is at least 90% identical to a sequence selected from the group consisting of: the polynucleotide sequence of Accession No. AJ875406 claim 39 , BT015128 claim 39 , AF127802 claim 39 , AJ249910 claim 39 , X59465 claim 39 , or X55392; a polynucleotide sequence encoding the amino acid sequence of Accession No. CAB76571; the polynucleotide ...

SYSTEMS AND METHODS FOR CLEANING WATER DISPENSERS

Systems and methods for cleaning a water dispenser by preparing a bioremediation solution with microbes, a growth medium, and water; injecting the bioremediation solution into a water supply; and flushing the water supply with the bioremediation solution. 1. A method for cleaning a water drip system , comprising:preparing a bioremediation solution with microbes, a growth medium, and water;injecting the bioremediation solution into a water supply; andflushing the water supply with the bioremediation solution.2. The method of claim 1 , comprising selecting a member of Bacillus as the microbe.3. The method of claim 1 , comprising:removing algae from drip line exit points; andremoving calcium from the drip line exit points to clean the water drip system.4. The method of claim 1 , wherein the growth medium comprises a carbon source claim 1 , sugar claim 1 , molasses claim 1 , or maltodextrin.5. The method of claim 1 , comprising mixing the solution with 1 part microbes claim 1 , 10 part carbon source claim 1 , and 1000 parts water.6. The method of claim 1 , comprising aerating the solution for at least six hours and then injecting the solution through a drip line.7. The method of claim 1 , comprising performing pulsed irrigation.8. The method of claim 1 , comprising cleaning emitters at a predetermined distance and flow per hour as per crop distance.9. The method of claim 1 , wherein the bioremediation solution is a highly concentrated form of at least 1×10cfu/ml.10BacillusBacidiceler, B. acidicola, B. acidiproducens, B. acidocaldarius, B. acidoterrestrisr, B. aeolius, B. aerius, B. aerophilus, B. agaradhaerens, B. agri, B. aidingensis, B. akibai, B. alcalophilus, B. algicola, B. alginolyticus, B. alkalidiazotrophicus, B. alkalinitrilicus, B. alkalisediminis, B. alkalitelluris, B. altitudinis, B. alveayuensis, B. alvei, B. amyloliquefaciens, B.amyloliquefaciens, B.plantarum, B. amylolyticus, B. andreesenii, B. aneurinilyticus, B. anthracia, B. aquimaris, B. arenosi, B. ...

COMPOSITIONS FOR ENHANCED ENZYME PRODUCTION

The present invention relates to compositions to induce production of proteins, e.g., enzymes, e.g., amylases or biomass degrading enzymes in a host cell, and methods for increasing the yield of the proteins, e.g., enzymes produced. Such compositions comprise a caramelized sugar product. The methods described herein can also be used to enhance processing of biomass materials, e.g., to produce sugar products. 1. A method for inducing production of a biomass degrading enzyme comprising contacting a microorganism that produces the biomass degrading enzyme with a composition comprising a caramelized sugar product under conditions sufficient for production of a biomass degrading enzyme.2. The method of claim 1 , wherein the microorganism is in a cell culture.3. The method of claim 2 , wherein sugar is added to the cell culture prior to contacting the microorganism with the composition comprising a caramelized sugar product.4. The method of claim 3 , wherein the microorganism is contacted with the composition comprising a caramelized sugar product when the cell culture is substantially free from sugar.5. The method of or claim 3 , wherein the caramelized sugar product is produced by caramelizing glucose claim 3 , xylose claim 3 , maltose claim 3 , lactose claim 3 , or a combination thereof.6. The method of claim 5 , wherein the caramelized sugar product produced by caramelizing saccharified biomass comprises xylose and glucose.7. The method of claim 5 , or claim 5 , wherein the caramelized sugar product comprises oligosaccharides claim 5 , dehydration products of the oligosaccharides claim 5 , hydration products of the oligosaccharides claim 5 , disproportionation products of the oligosaccharides claim 5 , colored aromatic products claim 5 , or any combination thereof.8. The method of claim 7 , wherein the oligosaccharides comprise disaccharides claim 7 , trisaccharides claim 7 , tetrasaccharides claim 7 , pentasacchrides claim 7 , hexasaccharides claim 7 , or a ...

Method for preparation of fungal mutant with high hydrolytic activity

A method for preparing a hyper-cellulolytic catabolite derepressed mutants of ascomycetes fungus, especially variants of Penicillium funiculosum . Selection media used to isolate such variants include amorphous cellulose and a high concentration of glucose. Cellulase activities of mutant ID-10, in particular such as FPase and β-glucosidase were 1.5 times higher than Penicillium funiculosum MRJ-16 (parent). Furthermore, fungal mutant morphology was changed and no pH adjustment was required throughout the enzyme production process.

PROCESS FOR PRODUCING LACTIC ACID OR ITS SALTS FROM FERMENTATION USING THERMOTOLERANCE BACILLUS BACTERIA

This invention relates to a process for producing lactic acid or its salts that can be performed easily, reduce complicated steps, and provide high lactic acid yield and high productivity, wherein said process comprising the following steps: (a) cultivating thermotolerance genus bacteria to obtain a seed culture; (b) increasing cell number of bacteria by inoculating the seed culture obtained from step (a) into a fermenter containing an initial carbon source under an aerobic condition; (c) fermenting the seed culture obtained from step (b) in the fermenter under a microaerobic condition to obtain lactic acid or its salts; wherein the step (b) comprising at least one addition of the carbon source under any one of the following conditions, which are independent to each other, to increase a concentration of the carbon source: —when the concentration of carbon source in the fermenter reduces to 50% or less comparing to the initial concentration. 1. A process for producing lactic acid or its salts , comprising the steps of:{'i': 'Bacillus', '(a) cultivating thermotolerance genus bacteria to obtain a seed culture;'}{'i': 'Bacillus', '(b) increasing the cell number of the thermotolerance genus bacteria by inoculating the seed culture obtained from step (a) into a fermenter containing an initial carbon source under an aerobic condition;'}(c) fermenting the seed culture obtained from step (b) in the fermenter under a microaerobic condition to obtain lactic acid or its salts; when the concentration of the carbon source in the fermenter reduces to 50% or less compared to the initial concentration;', 'when step (b) is carried out for a time of at least one third;', 'when an optical density (OD) of bacteria cells in the fermenter increases by at least 10 times., 'wherein step (b) further comprises at least one addition of the carbon source under any one of the following conditions, which are independent of each other, to increase the concentration of the carbon source2. The ...

Cells with improved pentose conversion

The invention relates to a cell capable of converting one or more pentose sugar and one or more hexose sugar into fermentation product constitutively expressing one or more heterologous or homologous polypeptide having the amino acid sequence set out in SEQ ID NO: 20, or a variant polypeptide thereof having at least 45% identity to SEQ ID NO 20. In an embodiment the heterologous polypeptide has glyoxalase activity.

SYSTEM AND METHOD OF BIOCATALYTIC CONVERSION FOR PRODUCTION OF ALCOHOLS, KETONES, AND ORGANIC ACIDS

Biocatalytic conversion systems and methods of producing and using same that have improved yields are disclosed. The systems and methods involve co-fermentation of sugars and gaseous substrates for alcohol, ketone, and/or organic acid production. The systems and methods may include biocatalytically converting at least one sugar substrate into at least one of alcohol, at least one ketone, and/or at least one organic acid. The systems and methods may further include biocatalytically converting gases that comprise COand Hto at least one alcohol and/or at least one organic acid, thereby adding extra revenue to biorefineries. 1. A biocatalytic conversion system that utilizes a co-fermentation process for sugar and gaseous substrates , the biocatalytic conversion system comprising:{'sub': 2', '2, 'a first reactor comprising at least one fermentation medium containing at least one first microorganism, wherein the at least one fermentation medium comprises at least one sugar substrate, and wherein the at least one first microorganism comprises a sugar fermenting species that converts sugars into at least one of acetone, butanol, ethanol, isopropanol, acetic acid, and butyric acid, and wherein a gaseous substrate comprising COand Hgases is produced during the fermentation process;'}{'sub': 2', '2, 'a second reactor comprising at least one medium containing at least one second microorganism, wherein the at least one second microorganism comprises a gas fermenting species that converts COand Hgases into at least one of an alcohol and an organic acid; and'}a gas line connecting the first reactor to the second reactor for feeding the gaseous substrate produced in the first reactor into the second reactor.2Clostridium, Butyribacterium, Eubacterium, Moorella, Acetobacterium, Enterobacter, Bacillus, Anaerobaculum, Alkalibaculum. The biocatalytic conversion system of claim 1 , wherein each of the first and second microorganisms comprises one or more species of microorganisms claim 1 ...

METHOD OF PRODUCING SUGAR LIQUID

A sugar liquid or solid sugar is derived from cellulose-containing biomass or blackstrap molasses, wherein content of one or more free amino acids selected from the group consisting of serine, threonine, asparagine, aspartic acid, glutamine, glutamic acid, proline, phenyl alanine, lysine, and histidine, which are impurities, is below the limit of detection. 1. A sugar liquid or solid sugar derived from cellulose-containing biomass or blackstrap molasses , wherein content of one or more free amino acids selected from the group consisting of serine , threonine , asparagine , aspartic acid , glutamine , glutamic acid , proline , phenyl alanine , lysine , and histidine , which are impurities , is below the limit of detection.2. A method of producing a chemical substance , comprising:(a) decomposing one or more fermentation inhibitor(s) contained in a sugar aqueous solution obtained from biomass with a microorganism incapable of utilizing glucose and/or xylose or a crude enzyme extract obtained from the microorganism, to obtain a sugar liquid having a reduced fermentation inhibitory activity compared to the sugar aqueous solution;(b) subjecting the sugar liquid obtained in step (a) to membrane concentration and/or evaporation concentration to increase sugar concentration; and(c) culturing a microorganism with the sugar liquid obtained in step (b) as a fermentation raw material to convert a sugar into the chemical substance,wherein the one or more fermentation inhibitor(s) are selected from the group consisting of coumaric acid, coumaramide, ferulic acid, ferulamide, vanillin, vanillic acid, acetovanillone, furfural, and β-hydroxymethylfurfural, and{'i': Delftia', 'Delftia, 'the microorganism incapable of utilizing glucose and/or xylose is a microorganism (sp.).'}3Delftia acidovorans, Delftia lacustris, Delftia tsuruhatensisDelftia litopenaei.. The method according to claim 2 , wherein the microorganism incapable of utilizing glucose and/or xylose is selected from the ...

pH CONTROLLED YEAST PROPAGATION

The invention relates to a process for the aerobic propagation of yeast wherein the yeast is grown in a reactor, comprising the following steps: a) filling the reactor with carbon source and an initial yeast population, b) optionally growing the initial yeast population in the reactor in batch mode, c) measuring the pH in the reactor, d) adding lignocellulosic hydrolysate to the reactor in fed batch mode at a rate to set the pH in the reactor at a predetermined value, and e) after sufficient propagation, isolation of yeast from the reactor. The invention further relates to yeast propagated according to that propagation process and to a process for the production of fermentation product wherein sugar comprising hexose and pentose is anaerobically fermented to fermentation product with the propagated yeast.

Increased Ethanol Production By Genetic Engineering Of Microorganisms To Express Hemoglobin

The present disclosure describes novel bacterial strains which express a pyruvate decarboxylase gene and at least one alcohol dehydrogenase gene from a bacteria of the genus Zymomonas and also express a hemoglobin gene from a bacteria of the genus Vitreoscilla . The present disclosure further describes methods for producing fermentation products with a microorganism which expresses a pyruvate decarboxylase gene and at least one alcohol dehydrogenase gene from a bacteria of the genus Zymomonas and also express a hemoglobin gene from a bacteria of the genus Vitreoscilla . Further the present disclosure describes methods for increasing production of a fermentation product comprising genetically engineering a microorganism which expresses a xylose isomerase gene to also express a hemoglobin gene from a bacteria of the genus Vitreoscilla.

A PROCESS FOR GROWING A MICROBIAL ORGANISM

An method for growing a microbial organism, comprising the cultivation of the microbial organism in the presence of a hydrolyzed composition obtained from a thermally treated ligno-cellulosic biomass. The treatment preferably comprises a fiber shives reduction step. The hydrolyzed composition has very few inhibitor compounds and the microbial organism feed with the hydrolyzed composition grows in a short time with a high duplication factor. 125-. (canceled)26. A process for growing a microbial organism comprising the steps of:a. thermally treating a ligno-cellulosic biomass feedstock to create a thermally treated ligno-cellulosic biomass, said thermally treated ligno-cellulosic biomass comprising xylans, glucans and lignin;b. dispersing an amount of the thermally treated ligno-cellulosic biomass into an amount of a carrier liquid to create a slurry;c. contacting the slurry with an enzyme under hydrolysis conditions of a carbohydrate component of the slurry to produce a hydrolyzed composition comprising simple sugar or sugars derived from the xylans and glucans of the thermally treated biomass, wherein the simple sugar or sugars can be metabolized by the microbial organism;d. cultivating the microbial organism in a cultivation environment comprising at least a portion of the hydrolyzed composition under conditions and for a cultivation time sufficient to grow the microbial organism.27. The process of claim 26 , wherein the thermally treated ligno-cellulosic biomass is in physical forms of at least fibres claim 26 , fines and fiber shives claim 26 , wherein:i. the fibres each have a width of 75 μm or less, and a fibre length greater than or equal to 200 μm,ii. the fines each have a width of 75 μm or less, and a fine length of less than 200 μm,iii. the fiber shives each have a shive width greater than 75 μm with a first portion of the fiber shives each having a shive length less than 737 μm and a second portion of the fiber shives each having a shive length greater ...

Methods of Mycological Biopolymer Production

Several types of non-agricultural lignocellulosic waste media are disclosed for the growth of mycological biopolymers. The growth medium is comprised of a substrate with a composition of appropriate Carbon, Nitrogen and mineral components including but not limited to lipids, proteins, and other inherent nutrition requisite for mycelial growth. Specific examples are () a lignocellulosic material, () a mineral based material, () a non-toxic, organic or inorganic, non-lignocellulosic material, () a synthetically sourced and produced material, () a whole tree (flourized), and () an agar media. 1. A method of growing a mycelial mat comprising the steps ofproviding a growth medium of non-agricultural lignocellulosic waste media;adding a nutrient for fungal growth and a fungal inoculum to said growth medium;thereafter placing said growth medium in a closed incubation chamber;maintaining said closed incubation chamber with a predetermined environment of humidity, temperature, carbon dioxide and oxygen sufficient to prevent the full differentiation of the fungus into a mushroom without producing a stipe, cap, or spores; andincubating the growth media in each said container for a period of time sufficient for said fungus to digest said nutritive substrate and produce a mycelium mat consisting entirely of fungal mycelium on said growth medium.2. A method as set forth in wherein said growth media is a lignocellulosic material in particle size.3. A method as set forth in wherein said lignocellulosic material has a particle size of no more than ¼ inch.4. A method as set forth in wherein said lignocellulosic material has a particle size in a range of from ¼ inch to 2 inches.5. A method as set forth in wherein said growth media is a mineral based material.6. A method as set forth in wherein said mineral based material is at least one of vermiculite claim 5 , perlite claim 5 , soils and chalk.7. A method as set forth in wherein said growth media is a non-toxic non-cellulosic ...

YEAST STRAINS ENGINEERED TO PRODUCE ETHANOL FROM ACETIC ACID AND GLYCEROL

The present invention relates to processes for producing ethanol from lignocellulosic hydrolysates comprising, hexoses, pentoses and acetic acid, whereby genetically modified yeast cells are use that comprise an exogenous gene encoding an acetaldehyde dehydrogenase and a bacterial gene encoding an enzyme with NAD-linked glycerol dehydrogenase activity. The process is further characterised in that glycerol is present in or fed into the culture medium, whereby the modified yeast cell ferments the hexoses, pentoses, acetic acid and glycerol to ethanol. The invention further relates to yeast cells for use in such processes. The yeast cells advantageously comprise genetic modifications that improve glycerol utilization such as modifications that increase one or more of dihydroxyacetone kinase activity and transport of glycerol into the cell. The yeast cell further preferably comprises a functional exogenous xylose isomerase gene and/or functional exogenous genes which confer to the cell the ability to convert L-arabinose into D-xylulose 5-phosphate and they may comprise a genetic modification that increase acetyl-CoA synthetase activity. 3. The process of claim 2 , wherein the yeast cell comprises at least one of:i) a functional exogenous xylose isomerase gene, which gene confers to the cell the ability to isomerise xylose into xylulose; andii) functional exogenous genes encoding a L-arabinose isomerase, a L-ribulokinase and a L-ribulose-5-phosphate 4-epimerase, which genes together confers to the cell the ability to convert L-arabinose into D-xylulose 5-phosphate.4. The process of claim 3 , wherein the yeast cell comprises at least one further genetic modification that result in a characteristic selected from the group consisting of:a) increased xylulose kinase specific activity;b) increased flux of the pentose phosphate pathwayc) reduced unspecific aldose reductase specific activityd) increased transport of at least one of xylose and arabinose into the host cell;e) ...

Ultrafiltration membrane based on bacterial nanocellulose and graphene oxide

The present disclosure is directed to ultrafiltration membranes based on bacterial nanocellulose and graphene oxide. In particular, the present disclosure is directed to the novel design and incorporation of membranes for realizing new, highly efficient, and environmentally-friendly anti-biofouling membranes for water purification.

Process for Solid-state Cultivation of Mycelium on a Lignocellulose Substrate

In one embodiment, the process combines a fraction (up to 15%) of a lignocellulose substrate with supplemental nutritional material and hydrates the fraction to a moisture content of from 40% to 70% by weight. The hydrated substrate fraction is heat processed to remove ambient bioburden (yeast, mold, bacteria) and to maintain the hydrated substrate fraction in an aseptic condition. Thereafter, the hydrated substrate fraction is inoculated with a fungus and incubated to obtain a myceliated substrate which is then reduced into discrete particles. The remaining fraction of the substrate is combined with water and then combined with the discrete particles of myceliated substrate and incubated to obtain a second myceliated substrate which is then reduced into discrete particles. The second myceliated substrate is combined with supplemental nutritional material and incubated to obtain a third myceliated substrate composed of at least 10% mycelium. 1. A process for solid-state cultivation of mycelium on a lignocellulose substrate comprising the steps ofobtaining a lignocellulose substrate;combining a first fraction of said substrate with supplemental nutritional material at a ratio of up to 14% of the dry mass of said fraction and hydrating said fraction to a moisture content of from 40% to 70% by weight;heat processing the hydrated substrate fraction for a period of time sufficient to eliminate ambient bioburden and to maintain the hydrated substrate fraction in an aseptic condition;thereafter inoculating the hydrated substrate fraction with a predetermined fungus;thereafter incubating the inoculated substrate for a period of time to allow said fungus to grow hyphae and to allow said hyphae to form a network of interconnected mycelia cells through and around discrete particles of said substrate fraction to obtain a myceliated substrate;thereafter reducing the myceliated substrate into discrete particles;combining the remaining fraction of said substrate with water to ...

Method for preparing a degradable material

The disclosure relates to the biotechnology field, and a method for preparing degradable materials, which comprises: inoculating Ceriporia lacerata into a culture medium containing lignocellulose debris for solid-state fermentation. The accession number of the C. lacerata is CGMCC No. 10485. The disclosure also relates to a degradable material obtained by the method and a method for cultivating the C. lacerata. The mycelia of the C. lacerata used herein can enwind and fix the culture medium debris (a lignocellulose material). By dint of the C. lacerata, the lignocellulose material can be prepared into a degradable material without sterilization or anti-bacterial treatment, and the preparation process doesn't require a sterile environment; thus, the preparation cost is decreased remarkably. The preparation process of the (plant-based) degradable material herein is energy saving and environment friendly, and the obtained degradable material is low cost and easy to degrade, and its properties are easy to control.

BIOLOGICAL CONVERSION OF BIOMASS-DERIVED SUGARS TO VALUE-ADDED CHEMICALS

A method of growing a microorganism by culturing the microorganism in a an aqueous solution of carbohydrates containing C6-sugar monomers or C5-sugar monomers, wherein the aqueous solution of carbohydrates is made by reacting biomass or a biomass-derived reactant with a solvent system including a lactone and water, and an acid catalyst. The reaction yields a product mixture containing water-soluble C6-sugar-containing oligomers, C6-sugar monomers, C5-sugar-containing oligomers, C5-sugar monomers, or any combination thereof. The product mixture is then partitioned or extracted to yield an aqueous layer containing the carbohydrates and a substantially immiscible organic layer containing the lactone. The aqueous layer is used for growing the microorganisms. 119-. (canceled)20. A method of growing a microorganism , the method comprising:(a) reacting biomass or a biomass-derived reactant with a solvent system comprising (i) an organic solvent selected from the group consisting of beta-lactones, gamma-lactones, delta-lactones, and combinations thereof, and (ii) at least about 1 wt % water, in the presence of an acid catalyst for a time and under conditions to yield a product mixture wherein at least a portion of water-insoluble C6-sugar-containing polymers or oligomers, or water-insoluble C5-sugar-containing polymers or oligomers, if present in the biomass or biomass-derived reactant, are converted to water-soluble C6-sugar monomers, C5-sugar monomers, or any combination thereof; and then(b) partitioning or extracting the product mixture into a liquid organic layer and a substantially immiscible liquid aqueous layer, wherein the substantially immiscible aqueous layer comprises an aqueous solution of carbohydrates comprising C6-sugar monomers, C5-sugar monomers, or a combination thereof; and(c) culturing the microorganism in a growth medium comprising the aqueous solution of carbohydrates.21. The method of claim 20 , wherein step (c) comprises culturing a microorganism ...

BIOLOGICAL CONVERSION OF BIOMASS-DERIVED SUGARS TO VALUE ADDED CHEMICALS

A method of growing a microorganism by culturing the microorganism in a an aqueous solution of carbohydrates containing C6-sugar monomers or C5-sugar monomers, wherein the aqueous solution of carbohydrates is made by reacting biomass or a biomass-derived reactant with a solvent system including a lactone and water, and an acid catalyst. The reaction yields a product mixture containing water-soluble C6-sugar-containing oligomers, C6-sugar monomers, C5-sugar-containing oligomers, C5-sugar monomers, or any combination thereof. The product mixture is then partitioned or extracted to yield an aqueous layer containing the carbohydrates and a substantially immiscible organic layer containing the lactone. The aqueous layer is used for growing the microorganisms. 1. A method of growing a microorganism , the method comprising:(a) culturing the microorganism in a growth medium comprising an aqueous solution of carbohydrates comprising C6-sugar monomers, C5-sugar monomers, or a combination thereof, wherein the aqueous solution of carbohydrates is made by process comprising(b) reacting biomass or a biomass-derived reactant with a solvent system comprising (i) an organic solvent selected from the group consisting of beta-, gamma-, and delta-lactones, and combinations thereof, and (ii) at least about 1 wt % water; in the presence of an acid catalyst for a time and under conditions to yield a product mixture wherein at least a portion of water-insoluble C6-sugar-containing polymers or oligomers, or water-insoluble C5-sugar-containing polymers or oligomers, if present in the biomass or biomass-derived reactant, are converted to water-soluble C6-sugar monomers, C5-sugar monomers, or any combination thereof; and then(c) partitioning or extracting the product mixture into an organic layer and a substantially immiscible aqueous layer which is used as the aqueous solution of carbohydrates comprising C6-sugar monomers, C5-sugar monomers, or a combination thereof.2. The method of claim 1 , ...

PAENIBACILLUS SP. CAA11 CAPABLE OF SACCHARIFICATION AND FERMENTATION OF CELLULOSE AND TRANSFORMED STRAIN THEREOF

The present disclosure relates to a novel strain capable of saccharifying and fermenting biomass-derived cellulose and a recombinant strain thereof with improved biomass saccharification capability. The present disclosure also relates to a method for producing a material useful as a bioenergy source material such as ethanol, acetic acid, formic acid, etc. using the strain or the recombinant strain. The strain or the recombinant strain may be usefully used in bioenergy industry. 1Paenibacillus. sp. CAA11 with a deposition number of KCCM 11602P.2PaenibacillusPaenibacillusBacillus subtilis. sp. CAA11-Cel , which is a genetically engineered strain of sp. CAA11 which has been transformed by a vector comprising the 168 cellulase gene.3PaenibacillusPaenibacillusPaenibacillus. The sp. CAA11-Cel according to claim 2 , wherein the genetically engineered strain of sp. CAA11 is a genetically engineered strain of sp. CAA11 with a deposition number of KCCM11825P.4PaenibacillusBacillus subtilis. The sp. CAA11-Cel according to claim 2 , wherein the 168 cellulase gene has a nucleotide sequence of SEQ ID NO 4.5PaenibacillusBacillus subtilis. The sp. CAA11-Cel according to claim 2 , wherein the vector comprises a promoter having a nucleotide sequence of SEQ ID NO 5 upstream of the 168 cellulase gene.6Paenibacillus. The sp. CAA11-Cel according to claim 2 , wherein the vector has a nucleotide sequence of SEQ ID NO 6.7Paenibacillus. A method for preparing the sp. CAA11-Cel according to claim 2 , which comprises:preparing an expression vector by inserting a promoter into a shuttle vector;preparing a recombinant vector by joining the promoter with a signal peptide and a cellulase-encoding gene by inserting them into the expression vector by overlap PCR and cloning the same; and{'i': 'Paenibacillus', 'transforming the recombinant vector into sp. CAA11.'}8. The method according to claim 7 , wherein said transforming further comprises performing electroporation.9Paenibacillus. A culture of ...

PROCESSING OF PLANT MATERIAL INTO BACTERIAL FEEDSTOCK

Methods and compositions relating to processing of plant material into bacterial feedstock, such as bacterial feedstock suitable for nanocellulose production, are disclosed. Cellulosic plant fiber may be contacted with a catalyst such as an acid catalyst or an enzymatic catalyst or both, and the mixture can be hydrolyzed into a hydrolysate. The hydrolysate may be used to form a culture medium which can be used to support bacterial growth to form the nanocellulose. 1. A method of making bacterial nanocellulose , the method comprising:contacting a cellulosic plant fiber with a catalyst selected from an acid catalyst, an enzymatic catalyst or a combination thereof to form a reaction mixture;subjecting the reaction mixture to hydrolyze at least a portion of the cellulosic plant fiber into a hydrolysate;forming a culture medium with the hydrolysate;inoculating the culture medium with at least one bacterium; andincubating the culture medium and the bacteria to produce the bacterial nanocellulose.2. The method of claim 1 , further comprising separating any unhydrolyzed cellulosic plant fiber from the hydrolysate and adding the unhydrolyzed cellulosic plant fiber to the reaction mixture.34.-. (canceled)5. The method of claim 1 , further comprising fragmenting the cellulosic plant fiber before contacting with the catalyst claim 1 , wherein the cellulosic plant fiber has an average particle size of about 250 μm to about 420 μm after the fragmenting step.6. (canceled)7Sorghum. The method of claim 5 , further comprising crushing a cellulosic plant to form the cellulosic plant fiber and a juice before the fragmenting step and adding the juice to the hydrolysate when forming the culture medium claim 5 , wherein the cellulosic plant fiber is bagasse claim 5 , sugar cane bagasse claim 5 , or both.810.-. (canceled)11. The method of claim 1 , wherein forming the culture medium comprises adding at least one nitrogen source claim 1 , at least one trace element claim 1 , or both to the ...

PRODUCTION OF MALONYL-COA DERIVED PRODUCTS VIA ANAEROBIC PATHWAYS

The present invention provides for novel metabolic pathways to convert biomass and other carbohydrate sources to malonyl-CoA derived products, such as hydrocarbons and other bioproducts, under anaerobic conditions and with the net production of ATP. More specifically, the invention provides for a recombinant microorganism comprising one or more native and/or heterologous enzymes that function in one or more engineered metabolic pathways to achieve conversion of a carbohydrate source to, e.g., long-chain hydrocarbons and hydrocarbon derivatives, wherein the one or more native and/or heterologous enzymes is activated, upregulated, downregulated, or deleted. The invention also provides for processes to convert biomass to malonyl-CoA derived products which comprise contacting a carbohydrate source with a recombinant microorganism of the invention. 1132-. (canceled)133. A recombinant yeast microorganism comprisingone or more engineered metabolic pathways to convert a carbohydrate source to a hydrocarbon, (a) the conversion of phosphoenolpyruvate to oxaloacetate by a phosphoenolpyruvate carboxykinase and', '(b) the conversion of oxaloacetate and acetyl-CoA to malonyl-CoA and pyruvate by a heterologous transcarboxylase Enzyme Commission Number 2.1.3.1;, 'wherein the one or more engineered metabolic pathways comprises'}wherein the one or more engineered metabolic pathways further comprises downregulation or deletion of native pyruvate decarboxylase, and wherein the one or more engineered metabolic pathway further comprises a heterologous pyruvate formate lyase, pyruvate dehydrogenase, pyruvate:ferredoxin oxidoreductase or pyruvate:NADP+oxidoreductase.134. The recombinant microorganism of claim 133 , wherein the conversion of a carbohydrate source to a hydrocarbon is under anaerobic or microaerophilic conditions.135. The recombinant microorganism of claim 133 , wherein said hydrocarbon is selected from the group consisting of:(a) an alkane;(b) an alkene;(c) an alkyne;(d) a ...

METHOD FOR PRODUCING SINGLE CELL OIL FROM LIGNOCELLULOSIC MATERIALS

The present disclosure relates to methods for producing microbial lipids. The present disclosure also relates to methods for producing microbial lipids using inhibitors obtainable from lignocellulosic materials to supress the proliferation of unwanted microorganisms in the fermentation broth. The method can therefore reduce the risk of having contaminating microbes establish in the system and the cultivation and thus higher yields of microbial lipids may be obtained. 1. A method for producing lipids , comprising:(i) providing a cultivation medium having a lignocellulosic hydrolysate,(ii) providing a fermentation broth by inoculating the cultivation medium of (i) with a first microbe, where said first microbe is an oleaginous microbe,(iii) incubating said medium inoculated with said first microbe to allow lipids to accumulate, wherein said fermentation broth includes at least one microbial growth inhibitor, and wherein said first microbe is tolerant to said microbial growth inhibitor(s), wherein said incubation is conducted under aerobic conditions.2. The method of claim 1 , wherein said fermentation broth comprises a second microbe claim 1 , which is intolerant to said at least one microbial growth inhibitor.3. The method according to claim 2 , wherein said second microbe is present in the cultivation medium provided in step (i) or contaminated the fermentation broth at step (ii) or (iii).4. The method according to claim 1 , wherein said second microbe is a non-oleaginous microbe.5. The method according to claim 1 , wherein said at least one microbial growth inhibitor is present in said fermentation broth at a concentration within range of tolerance of said first microbe and outside a range of tolerance of said second microbe.6. The method according to claim 1 , comprising:adding said at least one microbial growth inhibitor or adjusting the concentration of said at least one microbial growth inhibitor in the fermentation broth.7. The method according to claim 1 , ...

Methods for Enhancing the Degradation of Cellulosic Material with Chitin Binding Proteins

The present invention relates to methods for degrading or converting a cellulosic material and for producing substances from the cellulosic material.

LAYERED CELL CULTURE MEDIA PARTICLES AND METHODS OF MAKING THEREOF

The present invention is directed to dry cell culture media or feeds comprising layered particles. The less stable or sensitive components are separated spatially from reactive components in media, feeds, supplements, or concentrates due to layering. The invention relates to processes for preparing such layered compositions and methods of making cell culture compositions that are stable, thermally, photo-chemically and/or to gamma irradiation. The invention also relates to kits and culture systems using media layered particles. 1. A method of making a media or a feed composition for culturing cells , comprising:i) subjecting a dry powder to suspension in a moving column of a gas in a fluid bed apparatus;ii) introducing at least one solvent using a sprayer into the dry powder of step (i) to form a layered base particle;iii) drying the layered base particle, oriv) subjecting a dry powder to suspension in a moving column of a gas in a fluid bed apparatus;v) introducing a first solvent using a first sprayer onto the dry powder of step (iv) to form a base granule;vi) introducing at least one second solvent using a second sprayer onto the base granule of step (v) to form a layered base granule;vii) drying the layered base granule.2. (canceled)3. The method of making the media or the feed composition for culturing cells of claim 1 , wherein the dry powder is a basal media powder claim 1 , a complete media powder claim 1 , a cell culture feed claim 1 , a cell culture supplement claim 1 , a cell culture media or feed concentrate or an amino acid mixture; or wherein the amino acids are selected from one or more of the twenty amino acids claim 1 , their salts or derivatives thereof; or claim 1 , wherein the amino acids are selected one or more of glycine claim 1 , alanine claim 1 , arginine claim 1 , aspartic acid claim 1 , glutamic acid claim 1 , glutamine claim 1 , asparagine claim 1 , histidine claim 1 , leucine claim 1 , isoleucine claim 1 , methionine claim 1 , ...

USE OF BACTERIAL BIOFILMS IN OENOLOGY

A method for preparing a fermented drink is described. The method comprises the initiation of fermentation by inoculating the fermentable drink with fermentative bacteria in the form of biofilm. 111-. (canceled)12. A method for preparing a fermented drink comprising the initiation of fermentation by inoculating the fermentable drink with fermentative bacteria in the form of biofilm.13. The method according to wherein the fermentable drink is moreover inoculated with fermentative yeasts claim 12 , the bacteria and fermentative yeasts being in combination in the form of a biofilm.14. The method according to wherein the fermentation is a malolactic fermentation and the fermentative bacteria are lactic bacteria in the form of biofilm.15. The method according to wherein the fermented drink is selected from wines claim 12 , fruit and vegetable based drinks claim 12 , vinegars claim 12 , ciders or beers claim 12 , preferably the fermented drink is a wine.16Oenococcus, Lactobacillus, Pediococcus, Weissella, LeuconostocOenococcus oeni.. The method according to wherein the fermentative bacteria are selected from bacterial strains belonging to the genus or combinations thereof claim 12 , preferably the fermentative bacteria are17Saccharomyces, Schizosaccharomyces, Brettanomyces, Torulaspora, Candida, Metschnikowia, Kluyveromyces. The method according to wherein the fermentative yeasts are selected from strains belonging to the genus or combinations thereof.18. The method according to wherein the fermentative bacteria in the form of biofilm are inoculated in a form removed from the support claim 12 , or in a form adherent to the support.19. The method according to wherein the support is selected from wood claim 18 , preferably species of wood used as containing fermented liquid product claim 18 , cork claim 18 , stainless steel claim 18 , polystyrene claim 18 , silicone or polyethylene composites.20. The method according to wherein the fermentative bacteria in the form of ...

LACTIC ACID BACTERIA GENETICALLY MODIFIED TO EXPRESS ENZYMES OF THE CELLULOLYTIC SYSTEM

Lactic acid cell cultures for processing lignocellulose are disclosed. The bacterial culture may comprise a biomass composition and a population of lactic acid bacteria which comprises: 1. A bacterial culture comprising a biomass composition and a population of lactic acid bacteria which comprises:(i) a first population of lactic acid bacteria which has been genetically modified to express a secreted cellulase; and(ii) a second population of lactic acid bacteria which has been genetically modified to express a secreted xylanase, wherein the ratio of the first population: second population is selected such that the specific activity of cellulase:xylanase in the culture is greater than 4:1 or less than 1:4.2. A population of lactic acid bacteria which have been genetically modified to express a secreted cellulase and a secreted xylanase.3. A culture comprising the population of lactic acid bacteria of and a biomass composition.4. The culture of claim 3 , wherein a molar ratio of cellulase:xylanase in the culture is greater than 4:1 or less than 1:4.5. The culture of claim 1 , wherein said specific activity ratio of cellulase:xylanase in the culture is greater than 10:1 or less than 1:10.6. The culture of claim 5 , wherein said specific activity ratio of cellulase:xylanase in the culture is less than HO.7. The culture of claim 1 , wherein an expression plasmid for expressing said secreted cellulase and said secreted xylanase is a pSIP-derived expression plasmid.8. The culture of claim 1 , wherein said biomass comprises lignocelluloses.9. The culture of claim 1 , wherein said biomass comprises cellulose and hemicellulose.10. The culture of claim 9 , wherein said biomass further comprises lignin.11. The culture of claim 1 , wherein said biomass is selected from the group consisting of paper claim 1 , paper products claim 1 , paper waste claim 1 , wood claim 1 , particle board claim 1 , sawdust claim 1 , agricultural waste claim 1 , sewage claim 1 , silage claim 1 , ...

LACTIC ACID BACTERIA FOR THE PRODUCTION OF ETHANOL FROM BIOMASS MATERIAL

Lactic acid bacterial cultures, cell populations and articles of manufacture comprising same are disclosed for generating ethanol from lignocellulose. 1. A bacterial culture comprising a biomass composition and a population of lactic acid bacteria which comprises:(i) a first population of lactic acid bacteria which has been genetically modified to express a secreted cellulase;(ii) a second population of lactic acid bacteria which has been genetically modified to express a secreted xylanase, wherein the ratio of the first population:second population is selected such that the specific activity of cellulase:xylanase in the culture is greater than 4:1 or less than 1:4; and(iii) a third population of lactic acid bacteria which has been genetically modified to produce ethanol.2. A bacterial culture comprising a biomass composition and a population of lactic acid bacteria which comprises:(i) a first population of lactic acid bacteria which has been genetically modified to express a secreted cellulase;(ii) a second population of lactic acid bacteria which has been genetically modified to express a secreted xylanase, wherein the ratio of the first population:second population is selected such that the specific activity of cellulase:xylanase in the culture is greater than 4:1 or less than 1:4, wherein said first and/or said second population of lactic acid bacteria has been further genetically modified to produce ethanol.3. An article of manufacture comprising:(i) a first population of lactic acid bacteria which are genetically modified to express at least one fibrolytic enzyme; and(ii) a second population of lactic acid bacteria which are genetically modified to produce ethanol from C5 or C6 sugars.4. The article of manufacture of claim 3 , wherein said first population of lactic acid bacteria express a cellulase.5. The article of manufacture of claim 4 , further comprising a third population of lactic acid bacteria claim 4 , which are genetically modified to express a ...

Polypeptides Having Glucuronyl Esterase Activity and Polynucleotides Encoding Same

The present invention relates to isolated polypeptides having glucuronyl esterase activity, catalytic domains and polynucleotides encoding the polypeptides or catalytic domains. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides or catalytic domains.

OBTAINING HIGH-PERFORMANCE YEAST STRAINS FOR METABOLIZING ARABINOSE

The present invention relates to methods for obtaining yeast strains suitable for metabolizing arabinose, and to improved strains with good performance as regards their capacity to ferment arabinose as well as xylose and glucose, including in the presence of inhibitors such as acetic acid.

STABILIZED CHLORINE DIOXIDE FOR CONTAMINATION CONTROL IN ZYMOMONAS FERMENTATION

Though chlorine dioxide is generally used to control bacterial contamination, a method was developed which allows the use of stabilized chlorine dioxide (SCD) for controlling contamination during fermentation that uses the bacteria as the biocatalyst, even though is sensitive to chlorine dioxide. Parameters were identified for inoculating a composition for fermentation with cells after a time period has elapsed following SCD addition. 1. A fermentation medium composition comprising:a) fermentation medium comprising cellulosic biomass hydrolysate; andb) stabilized chlorine dioxide.2. A saccharification reaction slurry composition comprising:a) cellulosic biomass;b) at least one cellulase enzyme; andc) stabilized chlorine dioxide.3. The composition of or wherein the concentration of stabilized chlorine dioxide is initially at least about 10 mg/kg , with the amount of stabilized chlorine dioxide given in terms of the amount of chlorine dioxide that can be released upon complete activation of stabilized chlorine dioxide by acid.4. The composition of or wherein the composition contains solids in least about 20% solids based on dry weight of biomass to total composition weight.5. The composition of or wherein the cellulosic biomass is selected from the group consisting of corn cobs , corn husks , corn stover , grasses , wheat , wheat straw , barley straw , hay , rice straw , switchgrass , waste paper , sugar cane bagasse , sorghum , and woody plant cellulosic components. The invention relates to the fields of microbiology and fermentation. More specifically, methods were developed for controlling bacterial contamination in fermentations using stabilized chlorine dioxide when is used as the biocatalyst in a hydrolysate medium.Fuel ethanol produced from renewable resources is one of the long-term solutions to global fossil fuel shortages, rising energy costs, and global warming effects related to increased atmospheric carbon dioxide. Fuel ethanol from renewable resources is ...

Method for Producing Fungus Structures

A method for growing organically derived building materials in the form of a moldable substrate that can be engineered to serve a wide range of manufacturing and construction applications is presented. In particular, the embodiments consider a plurality of fungal molded shapes preferably grown from fungal inoculum and mechanically compressed at least once during the growing process as well as integration of structure support members to the fungal structure. The present invention provides a fungal substrate which could be molded, and easily and cheaply preprocessed to precise geometric specifications. The organically derived building materials also incorporate layers of structural reinforcements to improve load bearing and other structural capacities. 1. A method for growing organically derived building materials in the form of a moldable substrate to serve a wide range of manufacturing and construction applications , the method comprising the steps of:a) obtaining a lignocellulose based medium capable of supporting the growth of saprophytic fungi;b) mixing said lignocellulose based medium with water to reach a hydration level;c) inoculating said lignocellulose based medium with a fungal inoculum;d) allowing time for said inoculated lignocellulose based medium to become colonized to the extent that said inoculated lignocellulose based medium is transformed into a fungal mycelium without any secondary organisms displacing the process through infection;e) strictly regulating environmental conditions surrounding the lignocellulose based medium during said inoculation step and allowing step;f) adding a primary compressive pressure on the lignocellulose based medium of at least 100 PSI;g) reducing said primary compressive pressure; andh) drying said colonized fungal mycelium for a specific time period.2. The method of wherein said drying step renders the fungal inoculum biologically inert.3. The method of wherein said hydration level is between 33% and 66%.4. The method ...

Novel Clostridium Species That Converts Wheat Straw and Switchgrass Hydrolysates Into Butyric Acid

The present disclosure is directed to methods for producing butyric acid comprising fermenting a lignocellulosic biomass hydrolysate using strain RPT-4213 under anaerobic conditions using dilute acid-pretreated hydrolysates of wheat straw, corn fiber, corn stover, rice hull, and switchgrass, for example. 1C. tyrobutyricum. A method for producing butyric acid comprising fermenting a lignocellulosic biomass hydrolysate with a strain.2C. tyrobutyricum. The method of wherein the strain is RPT-4213.3. The method of wherein the lignocellulosic biomass hydrolysate is made from agricultural biomass residue selected from the group consisting of wheat straw claim 1 , corn fiber claim 1 , corn stover claim 1 , rice hull claim 1 , and switchgrass.4. The method of wherein the lignocellulosic biomass hydrolysate is wheat straw hydrolysate.5C. tyrobutyricum. A composition for use in a method for producing butyric acid comprising a seed culture of RPT-4213 and a fermentation medium.6C. tyrobutyricum. A system for producing butyric acid comprising a bioreactor claim 3 , a seed culture composition comprising RPT-4213 claim 3 , a lignocellulosic hydrolysate.7. The system of wherein said lignocellulosic hydrolysate is selected from the group consisting of wheat straw claim 6 , corn fiber claim 6 , corn stover claim 6 , rice hull claim 6 , and switchgrass. This application claims the benefit of U.S. Provisional Application No. 62/170,499, filed Jun. 3, 2015, which is incorporated herein by reference in its entirety.The invention relates to a composition comprising RPT-4213, method for producing butyric acid from glucose using a novel microorganism strain RPT-4213, and a system for producing butyric acid from lignocellulosic biomass hydrolysates using strain RPT-4213.Fermentation processes using microorganisms provide a promising path for converting biomass and agricultural wastes into chemicals and fuels. Hemicellulose biomass contains an abundance of pentose sugars that can be used to ...

METHOD FOR REDUCING THE VISCOSITY OF A NANOFIBRILLAR CELLULOSE HYDROGEL

The invention relates to a method for reducing the viscosity of a nanofibrillar cellulose hydrogel, wherein the method comprises mixing a nanofibrillar cellulose hydrogel with an aqueous growth medium for cell culture, wherein the aqueous growth medium contains one or more salts and optionally one or more sugars, using shearing forces so that a homogeneous dispersion is formed. The invention further relates to a dispersion comprising a nanofibrillar cellulose hydrogel and an aqueous growth medium for cell culture and to a use of an aqueous growth medium. 1. A method for reducing the viscosity of a nanofibrillar cellulose hydrogel , wherein the method comprises mixing a nanofibrillar cellulose hydrogel with an aqueous growth medium for cell culture , wherein the aqueous growth medium includes one or more salts and optionally one or more sugars , using shearing forces strong enough in order to disperse the nanofibrillar cellulose homogeneously in the mixture so that a homogeneous dispersion of the nanofibrillar cellulose is formed , wherein individual rafts of hydrogel are not observable visually or macroscopically in the homogeneous dispersion.2. The method according to claim 1 , wherein the method comprises mixing the nanofibrillar cellulose hydrogel with the aqueous growth medium using an apparatus selected from the group consisting of a blender claim 1 , a fluidizer claim 1 , a disperser and a homogenizer.3. The method according to claim 1 , wherein the aqueous growth medium comprises at least about 1-400 meq/l of ions of one or more salts.4. The method according to claim 1 , wherein the dispersion of the nanofibrillar cellulose hydrogel and the aqueous growth medium comprises about 10-400 meq/l of ions of one or more salts.5. The method according to claim 1 , wherein the aqueous growth medium comprises about 0.1-500 mmol/l of one or more sugars.6. The method according to claim 1 , wherein the dispersion of the nanofibrillar cellulose hydrogel and the aqueous ...

METHOD FOR PREPARING BETANIN

The application provides a method for preparing betanin comprising steps of raw material crushing, flash extraction, double centrifugations, macroporous resin purification, desorption, enzymatic hydrolysis, organic film decoloring, concentration, crystallization and drying. The application optimizes the quality control of key process such as extraction, separation, purification and decolorization, and can obtain the betanin product with purity more than 99%, and the product is white, no bitterness, less solvent residue, no pesticide residue, and stable quality. 1. A method for preparing betanin , comprising the steps:{'i': 'Rubus suatrssimus', '(1) providing a dry sample of S. Lee leaves, crushing and passing them through 40-60 mesh sieve, putting the obtained powder material into a flash extractor, in which water at the amount of 18-20 times by weight of the powder is added, extracting for 3 times, 1 minute for each time, and combining all extract solutions to get the total extract solution;'}(2) concentrating the total extract solution to a concentrate solution 5 times by weight of the raw leaves, centrifuging the concentrate solution through a disc centrifuge and a tube centrifuge to obtain a centrifugate, and adjusting the centrifugate to pH 5.0-6.0 using an acid solution;(3) passing the adjusted centrifugate through a macroporous resin column, adjusting the effluent to pH 7.0 using 0.2% sodium hydroxide solution, detecting the content of betanin and ending sample loading when the content is >1%; when the loading is completed, washing the column firstly using pure water until the effluent is colorless and clear, secondly using alkaline solution until the effluent is PH 11 to 12, thirdly using pure water until the effluent is PH 7.0-7.5, fourthly using an acid solution until the effluent is PH 2.0-2.5, and finally using water until the effluent is PH 7.0;(4) desorbing by 45%-50% ethanol solution, collecting the effluent from the time betanin is detected as >1% to ...

PROCESS FOR ENZYMATIC HYDROLYSIS OF LIGNOCELLULOSIC MATERIAL AND FERMENTATION OF SUGARS

The invention relates to a process for the preparation of a sugar and/or fermentation product from lignocellulosic material. 1. A process for preparation of a sugar product from lignocellulosic material , said process comprising:a) pretreating the lignocellulosic material, i) first treating the lignocellulosic material with an enzyme composition comprising a lytic polysaccharide monooxygenase and a polypeptide selected from the group consisting of a cellobiohydrolase, an endoglucanase, a beta-glucosidase, a beta-xylosidase, an endoxylanase and any combination thereof, then', 'ii) adding oxygen to the mixture comprising the lignocellulosic material and the enzyme composition, and thereafter', 'iii) adding additional lytic polysaccharide monooxygenase to the mixture comprising the lignocellulosic material and the enzyme composition, and, 'b) enzymatically hydrolysing the pretreated lignocellulosic material in an enzymatic hydrolysis to obtain the sugar product in a process comprisingc) optionally, recovering the sugar product.2. A process for preparation of a fermentation product from lignocellulosic material , comprising:{'claim-ref': {'@idref': 'CLM-00001', 'claim 1'}, 'a) performing a process according to ,'}b) fermenting the sugar product to produce the fermentation product; andc) optionally, recovering the fermentation product.3. The process according to claim 1 , wherein dry matter content of the lignocellulosic material in the enzymatic hydrolysis is from 10-40 wt %.4. The process according to claim 1 , wherein the enzyme composition comprises a lytic polysaccharide monooxygenase and/or the additional lytic polysaccharide monooxygenase is from a fungus.5. The process according to claim 1 , wherein the enzyme composition comprising a lytic polysaccharide monooxygenase and/or the additional lytic polysaccharide monooxygenase is added in the form of a whole fermentation broth of a fungus.6Rasamsonia.. The process according to claim 4 , wherein the fungus is7. The ...

SYSTEMS AND METHODS FOR CLEANING WATER DISPENSERS

Systems and methods for cleaning a water dispenser by preparing a bioremediation solution with microbes, a growth medium, and water; injecting the bioremediation solution into a water supply; and flushing the water supply with the bioremediation solution. 1. A method for cleaning a water drip system , comprising:preparing a bioremediation solution with microbes, a growth medium, and water;injecting the bioremediation solution into a water supply; andflushing the water supply with the bioremediation solution.2Bacillus. The method of claim 1 , comprising selecting a member of as the microbe.3. The method of claim 1 , comprising:removing algae from drip line exit points; andremoving calcium from the drip line exit points to clean the water drip system.4. The method of claim 1 , wherein the growth medium comprises a carbon source claim 1 , sugar claim 1 , molasses claim 1 , or maltodextrin.5. The method of claim 1 , comprising mixing the solution with 1 part microbes claim 1 , 10 part carbon source claim 1 , and 1000 parts water.6. The method of claim 1 , comprising aerating the solution for at least six hours and then injecting the solution through a drip line.7. The method of claim 1 , comprising performing pulsed irrigation.8. The method of claim 1 , comprising cleaning emitters at a predetermined distance and flow per hour as per crop distance.9. The method of claim 1 , wherein the bioremediation solution is a highly concentrated form of at least 1×10cfu/ml.10BacillusBacidiceler, B. acidicola, B. acidiproducens, B. acidocaldarius, B. acidoterrestrisr, B. aeolius, B. aerius, B. aerophilus, B. agaradhaerens, B. agri, B. aidingensis, B. akibai, B. alcalophilus, B. algicola, B. alginolyticus, B. alkalidiazotrophicus, B. alkalinitrilicus, B. alkalisediminis, B. alkalitelluris, B. altitudinis, B. alveayuensis, B. alvei, B. amyloliquefaciens, B. aamyloliquefaciens, B. aplantarum, B. amylolyticus, B. andreesenii, B. aneurinilyticus, B. anthracia, B. aquimaris, B. arenosi, B ...

SUGAR MIXTURES AND METHODS FOR PRODUCTION AND USE THEREOF

A sugar mixture comprising: monosaccharides; oligosaccharides in a ratio ≧0.06 to total saccharides; disaccharides in a ratio to total saccharides ≧0.05; pentose in a ratio to total saccharides ≧0.05; at least one alpha-bonded di-glucose; and at least one beta-bonded di-glucose. Also disclosed are methods to make and/or use such mixtures. 161-. (canceled)62. A de-acidified lignocellulosic hydrolysate comprising:monosaccharides;higher oligosaccharides comprising three or more sugar units;a ratio of pentose to total saccharides of ≧0.05 weight/weight;at least one alpha-bonded di-glucose;at least one beta-bonded di-glucose;a ratio of mineral acid to total saccharides of ≦0.03 weight/weight; anda ratio of total saccharides to water of ≧0.35 weight/weight.63. The hydrolysate of claim 62 , wherein a ratio of the higher oligosaccharides to total saccharides is ≦0.2 weight/weight64. The hydrolysate of claim 62 , wherein a ratio of at least one of the alpha-bonded di-glucose and the beta-bonded di-glucose to total saccharides is ≧0.01 weight/weight.65. The hydrolysate of claim 62 , wherein the at least one alpha-bonded di-glucose comprises maltose claim 62 , isomaltose or trehalose.66. The hydrolysate of claim 62 , wherein the at least one beta-bonded di-glucose comprises gentiobiose claim 62 , sophorose or cellobiose.67. The hydrolysate of claim 62 , wherein the monosaccharides comprise glucose claim 62 , galactose claim 62 , mannose claim 62 , xylose and arabinose.68. The hydrolysate of claim 62 , further comprising hexanol or 2-ethyl-1-hexanol.69. The hydrolysate of claim 62 , further comprising a microorganism.70. The hydrolysate of claim 69 , wherein the microorganism is a genetically modified microorganism.71. The hydrolysate of claim 62 , further comprising a catalyst claim 62 , wherein the catalyst is not an acid or an enzyme.72. The hydrolysate of claim 62 , wherein the hydrolysate is de-salted.73. A method comprising:{'claim-ref': {'@idref': 'CLM-00062', 'claim 62 ...

Novel Composition of Matter and Method for Stimulating the Growth of Beneficial Microorganisms

The invention describes a novel composition of matter obtained from the leaves of green plants, which is useful in promoting the growth of beneficial microorganisms. Specifically, that the invention describes a hydrolysate prepared from plant leaf biomass (leaf biomass hydrolysate or “LBH”) which dramatically stimulates the growth of beneficial microorganisms. Use of LBH as a fermentation substrate can also stimulate rapid production of organic acids and other organic compounds. LBH can be used as a substrate to promote the fermentation-based production of biobased industrial chemicals or biofuels, LBH can be utilized as a prebiotic to promote the growth of beneficial probiotic organisms. In addition, LBH may also be useful in stimulating the fermentation-based production of other products, examples of which include preservatives, antibiotics, antigens, vaccines, amino acids, vitamins, recombinant proteins, bioremediation treatments, and immobilized enzymes. 123-. (canceled)2433-. (canceled)3464-. (canceled)65. A method for producing leaf biomass hydrolysate (LBH) comprising the following steps:(a) disrupting one or more leaves from tobacco, spinach or alfalfa, or a mixture of these;(b) drying the disrupted leaves to a water activity level of approximately 0.85 or lower;(c) incubating the disrupted leaves in a liquid comprising water, wherein incubating comprises heating the disrupted leaves in the liquid to a temperature of from about 60° C. to about 90° C. for a period of time of ranging from about 15 minutes to about one (1) week; and(d) filtering the liquid after incubation to remove solids, and produce a liquid comprising soluble constituents of LBH.66. The method of claim 65 , which further comprises the step of drying the product of step (d) to a powder.67. The method of claim 65 , wherein at least one desirable leaf component is removed from the leaves during or after the leaves are disrupted.68. The method of claim 65 , wherein in step (c) the disrupted ...

Compositions for saccharification of cellulosic material

The present invention relates to enzyme compositions for high temperature saccharification of cellulosic material and to uses thereof.

YEAST CELL CAPABLE OF SIMULTANEOUSLY FERMENTING HEXOSE AND PENTOSE SUGARS

A method for preparing a yeast capable of simultaneously fermenting a pentose and a hexose sugar, comprising: (a) providing a yeast comprising: one or more heterologous genes encoding an enzyme of a pentose metabolic pathway; disruptions of a gene encoding a ribulose-phosphate 3-epimerase and of a gene encoding a glucose-6-phosphate isomerase; one or more overexpressed endogenous genes encoding an enzyme of the pentose phosphate pathway; and a disruption of one or more genes encoding an NADPH dependent 6-phosphogluconate dehydrogenase, (b) subjecting said yeast to evolutionary engineering on a medium comprising a hexose sugar and at least one pentose sugar, selecting for a yeast with improved growth rate obtain an evolved yeast; (d) restoring, in the evolved yeast, one or more of the disrupted genes, or: (d′) identifying genetic permutations in at least part of the genome of the evolved yeast by genome sequencing; (e) constructing an improved pentose and hexose-fermenting yeast comprising one or more said genetic permutations. Also described is a recombinant yeast comprising one or more heterologous genes of a pentose metabolic pathway, and a gene encoding a variant of a parent polypeptide, the variant comprising an amino acid sequence comprising at least one mutation, when aligned with the amino acid sequence in SEQ ID NO: 6. 1. A method for preparing a yeast which is capable of simultaneously fermenting a pentose and a hexose sugar , said method comprising: one or more heterologous genes encoding an enzyme of a pentose metabolic pathway;', 'a disruption of a gene encoding a ribulose-phosphate 3-epimerase and a disruption of a gene encoding a glucose-6-phosphate isomerase; and', 'one or more overexpressed endogenous genes encoding an enzyme of the pentose phosphate pathway; and optionally:', 'a heterologous gene encoding an NADH-dependent 6-phosphogluconate dehydrogenase; and', 'a disruption of one or more genes encoding an NADPH dependent 6-phosphogluconate ...

MICROBIAL STRAINS AND USES THEREOF

The present invention relates to strains comprising a mutation or deletion in the first and/or second XYL2 allele which can be used for producing one or more sugar alcohols from a lignocellulosic feedstock. The preferred sugar alcohol is xylitol. 1Candida. A strain comprising a mutation or deletion in the first and/or second XYL2 allele.2Candida. The strain as claimed in claim 1 , wherein the strain has a deletion of the first and/or second XYL2 allele.3CandidaCandidaCandida tropicalisCandida tropicalisCandida tropicalisScheffersomycesCandidashehataeScheffersomycesCandidashehataeScheffersomycesCandidashehatae. The strain as claimed in either or claim 1 , wherein the strain comprises NCYC 4185 or NCYC 4186 or NCYC 4190 or () NCYC 4187 or () NCYC 4188 or () NCYC 4189 or mutants or derivatives thereof.4Candida. The strain as claimed in any preceding claim claim 1 , for use in producing one or more sugar alcohols from a lignocellulosic feedstock.5Candida. The strain as claimed in claim 4 , wherein the one or more sugar alcohols produced comprises xylitol.6Candida. The strain as claimed in claim 5 , wherein the one or more sugar alcohols produced comprises xylitol and arabitol.7Candida. The strain as claimed in claim 6 , wherein the one or more sugar alcohols produced have a higher xylitol to arabitol ratio than strains without a mutation or deletion in the first and/or second XYL2 allele.8Candida. The strain as claimed in claim 7 , wherein the ratio of xylitol to arabitol is greater than about 2.0 fold.9Candida. The strain as claimed in claim 8 , wherein the ratio of xylitol to arabitol is about 2.7 fold.10Candida. The strain as claimed in claim 7 , wherein the ratio of xylitol to arabitol is about 4:1 or more.11Candida. The strain as claimed in claim 10 , wherein the ratio of xylitol to arabitol is higher after 24 hours of fermentation time than 48 hours of fermentation time.12Candida. The strain as claimed in any one of to claim 10 , wherein the one or more sugar ...

Biomass hydrothermal decomposition apparatus, temperature control method thereof, and organic raw material production system using biomass raw material

装置本体４２の一方側から固体のバイオマス原料１１を供給すると共に、他方側から加圧熱水１５を供給させ、両者を対向接触させつつバイオマス原料１１を水熱分解させ、熱水溶解成分を熱水中に溶解させ、装置本体４２の一方側から加圧熱水を熱水排出液１６として外部に排出すると共に、他方側からバイオマス固形分１７を外部に排出させるバイオマスの水熱分解装置であって、水熱分解を一定時間行った後に、温度を急降下させる内部温度冷却手段と、内部温度を計測する温度測定部Ｔ 1 〜Ｔ 8 と、温度測定部Ｔ 1 〜Ｔ 8 の温度計測結果を基に、内部温度冷却手段により内部温度を所定の冷却温度となるように制御する制御装置１００とを有する。 【選択図】図１−１ While supplying the solid biomass raw material 11 from one side of the apparatus main body 42, the pressurized hot water 15 is supplied from the other side, the biomass raw material 11 is hydrothermally decomposed while making the two face each other, and the hot water dissolved component is heated. This is a biomass hydrothermal decomposition apparatus that dissolves in water and discharges pressurized hot water from one side of the apparatus main body 42 as hot water discharge liquid 16 and discharges biomass solids 17 to the outside from the other side. Te, after the hydrothermal decomposition predetermined time, and the internal temperature cooling means for dive the temperature, a temperature measuring unit T 1 through T 8 for measuring the internal temperature, the temperature measurement result of the temperature measuring part T 1 through T 8 The control device 100 controls the internal temperature to a predetermined cooling temperature by the internal temperature cooling means. [Selection] Figure 1-1

在过氧化物酶存在下增加纤维素材料酶法水解的方法

本发明涉及用于增加纤维素材料水解的方法，包括在具有过氧化物酶活性的多肽存在下用酶组合物水解所述纤维素材料。

신규 미생물 바실러스 서브틸리스 서브스페시스 서브틸리스ａ-53 및 이를 이용한 섬유소 분해효소의 제조방법

본 발명은 신규 미생물 바실러스 서브틸리스 서브스페시스 서브틸리스 Ａ-53( Bacillus subtilis subsp. subtilis A-53) 및 이를 이용한 섬유소 분해효소의 제조방법에 관한 것으로, 보다 상세하게는, 해수로부터 동정한 바실러스 서브틸리스 서브스페시스 서브틸리스 Ａ-53 및 이를 배양하는 것을 특징으로 하는 섬유소 분해효소의 제조방법에 관한 것이다. 본 발명에 따른 신규 미생물은 사상균이나 곰팡이보다 생장속도가 빠르므로, 섬유소 분해효소를 경제적으로 제조하는 것이 가능하다. 바실러스 서브틸리스 서브스페시스 서브틸리스, 해양 미생물, 섬유소 분해효소, 생산

Гранулы, используемые в клеточной культуре, и способы их получения

РОССИЙСКАЯ ФЕДЕРАЦИЯ (19) RU (11) (13) 2021 132 093 A (51) МПК C12N 5/00 (2006.01) ФЕДЕРАЛЬНАЯ СЛУЖБА ПО ИНТЕЛЛЕКТУАЛЬНОЙ СОБСТВЕННОСТИ (12) ЗАЯВКА НА ИЗОБРЕТЕНИЕ (21)(22) Заявка: 2021132093, 16.12.2016 (71) Заявитель(и): ЛАЙФ ТЕКНОЛОДЖИЗ КОРПОРЕЙШН (US) Приоритет(ы): (30) Конвенционный приоритет: 17.12.2015 US 62/269,031 2018124447 04.07.2018 (43) Дата публикации заявки: 07.12.2021 Бюл. № 34 R U (57) Формула изобретения 1. Гранулированная клеточная культуральная среда, содержащая одну или более аминокислот, одно или более связующих веществ, один или более витаминов, одну или более солей и один или более следовых компонентов, где аминокислоты составляют 25-40%, связующие вещества составляют 20-65%, витамины составляют 1-5%, соли составляют 2-10% и следовые компоненты составляют 0,01-0,05%. 2. Гранулированная клеточная культуральная среда по п. 1, где витамины выбирают из одного или более из витамина B12, биотина, холина, фолиевой кислоты, ниацинамида, пиридоксина, рибофлавина, тиамина, аскорбиновой кислоты, пара-аминобензойной кислоты (PABA). 3. Гранулированная клеточная культуральная среда по п. 1, где соли выбирают из одной или более буферных солей, солей железа, цинка, кальция, меди, магния, марганца, аммония, ванадия. 4. Гранулированная клеточная культуральная среда по п. 1, где аминокислоты выбирают из одной или более из двадцати аминокислот, их солей или производных. 5. Гранулированная клеточная культуральная среда по п. 4, где аминокислоты выбирают из одной или более из глицина, аланина, аргинина, аспарагиновой кислоты, глутаминовой кислоты, гистидина, изолейцина, метионина, фенилаланина, пролина, гидроксипролина, серина, треонина, триптофана, валина, тирозина, цистеина и лизина. 6. Гранулированная клеточная культуральная среда по п. 1, где связующие вещества выбирают из группы, состоящей из сахара, природного вещества, синтетического вещества и полусинтетического вещества, микрокристаллической целлюлозы, глюкозы, сахарозы, трегалозы, моносахарида, ...

使用耐热性芽孢杆菌属细菌发酵来生产乳酸或其盐的方法

本发明涉及一种用于生产乳酸或其盐的方法，所述方法可以容易地进行，减少复杂的步骤，并提供高乳酸产率和高生产力，其中所述方法包括以下步骤：(a)培养耐热性芽孢杆菌属细菌以获得菌种培养物；(b)通过在好氧条件下将从步骤(a)获得的所述菌种培养物接种到含有初始碳源的发酵罐中来增加细菌的细胞数量；(c)在所述发酵罐中在微氧条件下发酵从步骤(b)获得的所述菌种培养物，以获得乳酸或其盐；其中所述步骤(b)包括在彼此独立的任一种以下条件下添加所述碳源至少一次，以增加所述碳源的浓度：‑当所述发酵罐中的碳源的所述浓度降低至与初始浓度相比50％或以下时。

Reservoir treatments

Provided herein is a method of establishing a plug in a hydrocarbon reservoir, the method comprising introducing into the reservoir a formulation comprising solid particles and a viscosifier and then reducing the viscosity of said viscosifier, thereby causing said solid particles to form a plug within said hydrocarbon reservoir. Also provided is a method of establishing a plug in a hydrocarbon reservoir, the method comprising introducing into the reservoir a formulation comprising: (a) microorganisms or cell-free enzymes; (b) solid particles; and (c) a viscosifier which is a substrate for the microorganisms or cell-free enzymes of (a). Also provided is a formulation comprising: (a) microorganisms or cell-free enzymes; (b) solid particles made from wood or a wood derived product; and (c) a viscosifier which is a substrate for the microorganisms or cell-free enzymes of (a).

Removal of inhibitors of microbial fermentation from inhibitor-containing compositions

Methods are provided for conditioning an inhibitor-containing composition, such as a cellulosic biomass hydrolysate, to remove inhibitors of microbial growth and/or product production. The methods include precipitation of inhibitors by formation of complexes with metal salts, such as aluminum sulfate and ferric chloride.

Lignocellulosic conversion processes and products

Processes for converting lignocellulose to feedstock and downstream products are disclosed. The processes may include acid treatment of lignocellulose to produce a fermentation feedstock. In various instances, the processes include recovery or recycling of acid, such as recovery of hydrochloric acid from concentrated and/or dilute streams. Downstream products may include acrylic acid-based products such as diapers, paper and paper-based products, ethanol, biofuels such as biodiesel and fuel additives, and detergents.

Trametes hirsuta basidiomycete strain - ethyl alcohol producer

FIELD: biotechnology. SUBSTANCE: strain of the basidial fungus Trametes hirsute, which has the ability to produce ethyl alcohol, was deposited in the Russian National Collection of Industrial Microorganisms under accession number VKPM F-1287. EFFECT: Trametes hirsute strain VKPM F-1287 allows to obtain bioethanol in high yield, which does not contain impurities such as acetone and isopropanol. 1 tbl, 5 ex РОССИЙСКАЯ ФЕДЕРАЦИЯ (19) RU (11) (13) 2 630 997 C1 (51) МПК C12P 7/10 (2006.01) C12N 1/14 (2006.01) C12N 1/22 (2006.01) ФЕДЕРАЛЬНАЯ СЛУЖБА ПО ИНТЕЛЛЕКТУАЛЬНОЙ СОБСТВЕННОСТИ (12) ОПИСАНИЕ ИЗОБРЕТЕНИЯ К ПАТЕНТУ (21)(22) Заявка: 2016150491, 21.12.2016 (24) Дата начала отсчета срока действия патента: 21.12.2016 Дата регистрации: Приоритет(ы): (22) Дата подачи заявки: 21.12.2016 (45) Опубликовано: 15.09.2017 Бюл. № 26 (73) Патентообладатель(и): Федеральное государственное бюджетное образовательное учреждение высшего образования "Российский государственный университет нефти и газа (национальный исследовательский университет) имени И.М. Губкина" (RU) (56) Список документов, цитированных в отчете о поиске: КОЖЕВНИКОВА Е.Ю. C 1 2 6 3 0 9 9 7 R U (54) ШТАММ БАЗИДИОМИЦЕТА TRAMETES HIRSUTA - ПРОДУЦЕНТ ЭТИЛОВОГО СПИРТА (57) Реферат: Изобретение относится к биотехнологии. под регистрационным номером ВКПМ F-1287. Штамм базидиального гриба Trametes hirsute, Штамм Trametes hirsute ВКПМ F-1287 позволяет обладающий способностью продуцировать получать биоэтанол с высоким выходом, не этиловый спирт, депонирован во Всероссийской содержащим такие примеси, как ацетон и Коллекции Промышленных Микроорганизмов изопропанол. 1 табл., 5 пр. Стр.: 1 C 1 Разработка технологических основ получения биоэтанола с использованием базидиальных грибов // Автореферат канд. хим. наук, М., 07.10.2016. JP 2010183859 A, 22.10.2014. WO 2010/044603 A1, 22.04.2010. US 2014/0154763 A1, 05.06.2014. 2 6 3 0 9 9 7 Адрес для переписки: 119991, Москва, Ленинский просп., 65, корп. 1, РГУ нефти и газа (НИУ) имени И.М. ...

Method of processing vegetable raw material for producing biomass of fodder yeast and/or ethyl alcohol

A cellulose-containing starting substance is pre-treated with a dilute mineral acid and/or a dilute base, and then fermented under aerobic or anaerobic conditions in the presence of a Candida utilis var. cellulolytica strain deposited at the Hungarian National Collection of Microorganisms of the National Institute for Public Health on 23rd September, 1980 under No. CU 28 00199. When fermentation is performed under aerobic conditions, fodder yeast or a mixture of fodder yeast and ethanol is obtained, depending on the aeration rate, whereas when fermentation is performed under anaerobic conditions, ethanol is obtained.

Yeast strains engineered to produce ethanol from acetic acid and glycerol

The present invention relates to processes for producing ethanol from lignocellulosic hydrolysates comprising hexoses, pentoses and acetic acid, whereby genetically modified yeast cells are use that comprise an exogenous gene encoding anacetaldehyde dehydrogenase. The process is further characterised in that glycerol is present in or fed into the culture medium, whereby the modified yeast cell ferments the hexoses, pentoses, acetic acid and glycerol to ethanol. The invention further relates to yeast cells for use in such processes. The yeast cells advantageously comprise genetic modifications that improve glycerol utilization such as modifications that increase one or more of glycerol dehydrogenase activity, dihydroxyacetone kinase activity and transport of glycerol into the cell. The yeast cell further preferably comprises a functional exogenous xylose isomerase gene and/or functional exogenous genes which confer to the cell the ability to convert L-arabinose into D-xylulose 5-phosphate and they may comprise a genetic modification that increase acetyl-CoA synthetase activity.

Yeast strains engineered to produce ethanol from acetic acid and glycerol

The present invention relates to processes for producing ethanol from lignocellulosic hydrolysates comprising hexoses, pentoses and acetic acid, whereby genetically modified yeast cells are use that comprise an exogenous gene encoding an acetaldehyde dehydrogenase and a bacterial gene encoding an enzyme with NAD + - linked glycerol dehydrogenase activity. The process is further characterised in that glycerol is present in or fed into the culture medium, whereby the modified yeast cell ferments the hexoses, pentoses,acetic acid and glycerolto ethanol. The invention further relates to yeast cells for use in such processes. The yeast cells advantageously comprise genetic modifications that improve glycerol utilization such as modifications that increase one or more of dihydroxyacetone kinase activity and transport of glycerol into the cell. The yeast cell further preferably comprisesa functional exogenous xylose isomerase gene and/or functional exogenous genes which confer to the cell the ability to convert L-arabinose into D-xylulose 5-phosphate and they may comprise a genetic modification that increase acetyl-CoA synthetase activity.

Microorganisms having enhanced tolerance to inhibitors and stress

The present invention provides genetically modified strains of microorganisms that display enhanced tolerance to stress and/or inhibitors such as sodium acetate and vanillin. The enhanced tolerance can be achieved by increasing the expression of a protein of the Sm-like superfamily such as a bacterial Hfq protein and a fungal Sm or Lsm protein. Further, the present invention provides methods of producing alcohol from biomass materials by using the genetically modified microorganisms of the present invention.

expression cassette, genetically modified microorganism for xylose isomerase expression, process for production of biofuels and / or biochemicals and biofuel and / or biochemicals produced

resumo cassete de expressão, micro-organismo geneticamente modificado para expressão de xilose isomerase, processo para produção de biocombustíveis e/ou bioquímicos e biocombustível e/ou bioquímicos produzidos a presente invenção refere-se a cassete de expressão que compreende um peptídeo com função xilose isomerase que possua pelo menos 70% de identidade com a sequência seq id no:1 e ao menos um promotor para a referida sequência codificante. a presente invenção também se refere a micro-organismo geneticamente modificado para expressão de xilose isomerase e que expressa pelo menos um peptídeo com pelo menos 70% de identidade com a sequência seq id no:1. a presente invenção também se refere a um processo para produção de biocombustíveis e/ou bioquímicos e ao biocombustível e/ou biquímicos produzidos pelo micro-organismo ou pelo processo da presente invenção.

Yeast strains engineered to produce ethanol from acetic acid and glycerol

The present invention relates to processes for producing ethanol from lignocellulosic hydrolysates comprising hexoses, pentoses and acetic acid, whereby genetically modified yeast cells are use that comprise an exogenous gene encoding an acetaldehyde dehydrogenase and a bacterial gene encoding an enzyme with NAD+-linked glycerol dehydrogenase activity. The process is further characterised in that glycerol is present in or fed into the culture medium, whereby the modified yeast cell ferments the hexoses, pentoses, acetic acid and glycerol to ethanol. The invention further relates to yeast cells for use in such processes. The yeast cells advantageously comprise genetic modifications that improve glycerol utilization such as modifications that increase one or more of dihydroxyacetone kinase activity and transport of glycerol into the cell. The yeast cell further preferably comprises a functional exogenous xylose isomerase gene and/or functional exogenous genes which confer to the cell the ability to convert L-arabinose into D-xylulose 5-phosphate and they may comprise a genetic modification that increase acetyl-CoA synthetase activity.

Nuruk containing salicornia herbacea and preparation method of the same

누룩곰팡이에 의한 쌀과 함초의 혼합 발효물을 포함하는 함초가 함유된 함초누룩 및 상기 누룩의 제조방법을 제공한다. 상기 누룩은 함초가 함유된 신규한 누룩으로, 식품분야 등에서 다양하게 활용가능하다. Provided is a hamcho malt containing hamcho containing mixed fermentation product of rice and hamcho by the yeast fungus and a method of producing the yeast. The Nuruk is a novel Nuruk containing a seaweed, it can be used in various ways in the food field.

Recombinant Thraustochytrids that Grow on Xylose, and Compositions, Methods of Making, and Uses Thereof

The present invention is directed to recombinant thraustochytrids that grow on xylose and cell cultures comprising the recombinant thraustochytrids as well as methods of producing cell cultures, biomasses, microbial oils, compositions, and biofuels using the recombinant thraustochytrids.