СПОСОБ ПОЛУЧЕНИЯ АСТАКСАНТИНА ПУТЕМ ФЕРМЕНТАЦИИ

Изобретение относится к способу продуцирования каротиноидов, в том числе астаксантина. Способ предусматривает культивирование бактерии, принадлежащей к роду Paracoccus, которая одновременно продуцирует астаксантин и кантаксантин, в среде, содержащей биотин в концентрации 0,001-50 мг/л. При этом концентрация растворенного кислорода в продукте культивирования поддерживается на уровне 1 м.д. или более во время культивирования, а отношение концентрации продуцируемого кантаксантина к концентрации продуцируемого астаксантина в продукте культивирования после завершения культивирования составляет 25 мас.% или менее. Изобретение обеспечивает получение астаксантина в высокой концентрации с пониженным уровнем продуцирования кантаксантина. 6 з.п. ф-лы, 9 табл., 6 пр.

(-) ШТАММ ГЕТЕРОТАЛЛИЧНОГО ФИКОМИЦЕТА Blakeslea trispora, ПРОДУЦИРУЮЩИЙ ЛИКОПИН В ПАРЕ С РАЗНЫМИ (+)ШТАММАМИ Blakeslea trispora, И СПОСОБ МИКРОБИОЛОГИЧЕСКОГО СИНТЕЗА ЛИКОПИНА

Изобретение относится к биотехнологии и касается микробиологического синтеза ликопина. Штамм Blakeslea trispora ВКПМ F-822 в паре с разными (+) штаммами Blakeslea trispora способен синтезировать ликопин. Способ синтеза ликопина на основе культивирования пары (-) и (+) штаммов Blakeslea trispora, где в качестве (-) штамма используют штамм Blakeslea trispora ВКПМ F-822, осуществляют на специально разработанной питательной среде следующего состава, %: мальтозного сиропа 8,0-14,0, кукурузного экстракта 4,0-8,0, дрожжей пекарских (сухих) 0,03-0,07, КН2РО4 0,04-0,06, NaCl 0,1-0,5, MnSO47H2O 0,005-0,015, тиамина 0,0002, растительного масла 3,0-6,0 воды водопроводной 84,8-71,4, рН среды до стерилизации 7,5-8,4. Предложенный (-) штамм в совокупности с предложенным способом обеспечивает высокий уровень синтеза ликопина до 1,7 г/л, позволяет осуществлять процесс без добавления в культуральную среду специальных химических соединений, стимулирующих ликопинобразование. 2 с. п.ф-лы, 2 табл.

СТАБИЛЬНЫЕ И БИОДОСТУПНЫЕ КОМПОЗИЦИИ ИЗОМЕРОВ ЛИКОПЕНА ДЛЯ КОЖИ И ВОЛОС

Изобретение относится к способу получения стабильной композиции, обогащенной цис-ликопеном (Z-изомеры). Способ проводят путем продолжительного нагревания в растворителе материала, содержащего ликопен, где температура нагревания составляет от 50 до 150°С, а время нагревания составляет от 4 до 240 часов. Изобретение позволяет получить продукт с высокой биодоступностью и стабильной изомерной композицией. 3 з.п. ф-лы, 1 ил., 4 табл.

СПОСОБ КУЛЬТИВИРОВАНИЯ МИКРОВОДОРОСЛИ COELASTRELLA RUBESCENS ДЛЯ ПОЛУЧЕНИЯ КАРОТИНОИДОВ И ЛИПИДОВ

Изобретение относится к биотехнологии микроводорослей и может быть использовано для получения каротиноидов и липидов. Предложен способ культивирования микроводорослидля одновременного получения кетокаротиноидов группы астаксантина и липидов для производства биодизеля. В способе предусмотрено культивированиеметодом двухстадийной накопительной культуры на питательной среде с соблюдением на I («зеленой») стадии режима освещения 15 ч свет/9 ч темнота. Перед культивированием на II («красной») стадии на среде ВВМ культуру подвергают комплексному стресс-воздействию - разведению полученной биомассы питательной средой ВВМ, редуцированной по азоту и фосфору, и переходят на круглосуточный режим освещения. На I («зеленой») стадиикультивируют в течение 11 суток на питательной среде МВВМ при n=1.1-1.3 кл⋅мли рН 7, соблюдая условия культивирования: I-140 μE⋅м⋅с, Т - 25-26°С, скорость продувки воздухом - 1 л⋅мин⋅л. На II («красной») стадии культивирования выполняют 11-кратное разведение культуры, которую ...

ПРОЦЕСС ЭКСТРАКЦИИ БЕЗ РАСТВОРИТЕЛЯ

... 1. Процесс получения липида из микроорганизмов, включающий (а) лизис клеток микроорганизмов в целях получения смеси растворенных клеток; (б) обработку указанной смеси растворенных клеток в целях получения смеси с разделенными фазами, включающей тяжелый слой и легкий слой, отличающуюся тем, что указанный тяжелый слой содержит водный раствор, а указанный легкий слой содержит указанный липид; (в) отделение указанного тяжелого слоя от указанного легкого слоя; а также (г) получение указанного липида из указанного легкого слоя. 2. Процесс по п.1, отличающийся тем, что стадия (б) включает центрифугирование указанной смеси растворенных клеток. 3. Процесс по п.2, отличающийся тем, что указанный легкий слой содержит эмульгированный липид. 4. Процесс по п.3, включающий также (д) добавление водного экстракционного раствора к указанному легкому слою со стадии (в); и (е) прежде стадии (г) повторение указанных стадий (в), (г) и (д) до тех пор, пока указанный липид не станет в значительной степени неэмульгированным ...

Способ получения зеаксантина

Способ получения @ -каротина из суспензии водорослей рода DUNaLIeLLa в растворе хлорида натрия с концентрацией не менее 3 М

Изобретение относится к биотехнологии и касается получения витаминов. Целью изобретения является улучшение качества β-каротина и упрощение процесса. Это достигается тем, что водоросли рода DUNALIELLA в растворе хлорида натрия концентрацией не менее 3М контактируют с гидрофобным корпускулярным или волокнистым адсорбентом, обрабатывают водоросли растворителем для экстракции β-каротина, свободного от нежелательных каротиноидов и липидов, а затем отгоняют растворитель из экстракта.

ASTAXANTIN LIEFERNDE HEFEZELLEN, VERFAHREN ZUR HERSTELLUNG.

VERFAHREN ZUR VITAMINHERSTELLUNG

In vivo extraction of secondary metabolite from micro algae comprises culturing algal biomass in aqueous culture medium for culturing micro algae, immobilizing biomass on solid substrate, and exposing substrate to solvent for extracting

In vivo extraction of secondary metabolite from micro algae comprises culturing algal biomass in an aqueous culture medium for culturing micro algae; immobilizing the cultured algal biomass on a solid substrate; and exposing the solid substrate with the immobilized algal biomass to the solvent that retains the vitality of the micro algae, for extracting the secondary metabolite, where the immobilization takes place by embedding the cultured algal biomass in a porous layer applied on the solid substrate.

Process for the recovery of carotenoid pigment from algae

Metabolically engineered cells for the production of resveratrol or an oligomeric or glycosidically-bound derivative thereof

PRODUCTION OF ZEAXANTHIN

... 1467303 Zeaxanthin NESTLE SA 17 July 1974 [26 July 1973] 31647/74 Heading C2V In a process for preparing zeaxanthin (3,31- dihydroxy-#-carotene) by culturing a suitable microorganism of the genus Flavobacter in an aqueous nutrient medium, the yield is improved by adding pyridoxine to the medium. Bivalent iron, cobalt, molybdenum or magnanese ions and methionine, cystine or cysteine also may be added. In examples the media comprise glucose, casein hydrolysate, yeast extract, magnesium sulphate, pyridoxine, a ferrous salt and methionine.

Production of Dunaliella

BIOGAS PROCESS WITH NUTRIENT RECOVERY

BIOGAS PROCESS WITH NUTRIENT RECOVERY

BIOGAS PROCESS WITH NUTRIENT RECOVERY

PRODUCTION OF ZEAXANTHIN AND BETA CRYPTOXANTHIN BY PHAFFIA

METHANOTROPHER BACTERIA TRUNK METHYLOMONAS 16A WITH HIGH GROWTH RATE

PROCEDURE FOR THE PRODUCTION OF TERPENEN AND MEPTRANSFORMIERTE MICROORGANISMS FOR IT

PROCEDURE FOR THE PRODUCTION OF CAROTENOID PIGMENTS

PROCEDURE FOR IN VIVO PRODUCTION OF ASTAXANTHIN AND PHAFFIA RHODOZYMA YEAST WITH INCREASED ONE ASTAXANTHINGEHALT

Procedure for the micro-biological production of Lycopin

Procedure for the production of Zeaxanthin

KETOGRUPPE EINFÜHRENDES ENZYME, BUT CODING DNA AND PROCEDURE FOR THE PRODUCTION OF KETOCAROTENOID

PHAFFIA RHODOZYMA MUTANTEN, PROCEDURES FOR THE PRODUCTION OF BETA CAROTIN AND USE OF BETA CAROTIN REALMS A BIOMASS

ASTAXANTHIN SYNTHETASE

METHOD FOR PRODUCING KETOCAROTENOIDS BY CULTIVATING GENETICALLY MODIFIED ORGANISMS

Use of astaxanthin-containing plants or parts of plants of the genus Tagetes as animal feed

Metabolically engineered cells for the production of resveratrol or an oligomeric or glycosidically-bound derivative thereof

Extraction of extracellular terpenoids from microalgae colonies

The invention provides methods of extracting and quantifying extracellular terpenoid hydrocarbons, e.g., botryococcenes, methylated squalenes, and carotenoids, from terpenoid-producing and secreting green microalgae.

Astaxanthin production using fed-batch fermentation process byphaffia rhodozyma

Modified cyanobacteria

PROCESS FOR CONTINUOUS PRODUCTION AND EXTRACTION OF CAROTENOIDS FROM NATURAL SOURCES

Carotenoid production from a single carbon substrate

Process for continuous production and extraction of carotenoids from natural sources

METHOD FOR THE PRODUCTION OF ZEAXANTHIN AND/OR THE BIOSYNTHETIC INTERMEDIATES AND/OR SUBSEQUENT PRODUCTS THEREOF

Production of acetyl-coenzyme a derived isoprenoids

Provided herein are compositions and methods for the heterologous production of acetyl-CoA-derived isoprenoids in a host cell. In some embodiments, the host cell is genetically modified to comprise a heterologous nucleotide sequence encoding an acetaldehyde dehydrogenase, acetylating (ADA, E.C. 1.2.1.10) and an MEV pathway comprising an NADH-using HMG-CoA reductase. In some embodiments, the host cell is genetically modified to comprise a heterologous nucleotide sequence encoding an ADA and an MEV pathway comprising an acetoacetyl-CoA synthase. In some embodiments, the genetically modified host cell further comprises one or more heterologous nucleotide sequences encoding a phosphoketolase and a phosphotransacetylase. In some embodiments, the genetically modified host cell further comprises a functional disruption of the native PDH-bypass. The compositions and methods described herein provide an energy-efficient yet redox balanced route for the heterologous production of acetyl-CoA-derived ...

Process for the Enzymatic Synthesis of (7S)-3,4-dimethoxybicyclo[4.2.0]octa-1,3,5-triene-7-carboxylic Acid or Esters thereof, and Application in the Synthesis of Ivabradine and salts thereof

PROCESS FOR THE ENZYMATIC SYNTHESIS OF (7S)-3,4 DIMETHOXYBICYCLO[4.2.0]OCTA-1,3,5-TRIENE-7-CARBOXYLIC ACID OR ESTERS THEREOF, AND APPLICATION IN THE SYNTHESIS OF IVABRADINE AND SALTS THEREOF Abstract A process for the enzymatic synthesis of the compound of formula (I): H3C-O s) H3C-O O Rio wherein R1 represents a hydrogen atom or an alkyl group, is disclosed. Also disclosed is its application in the synthesis of ivabradine and addition salts thereof with a pharmaceutically acceptable acid.

Process for the preparation of a granular microbial biomass and isolation of valuable compounds therefrom

PIGMENTATION SUPPLEMENTS FOR ANIMAL FEED COMPOSITIONS

Genes of carotenoid biosynthesis and metabolism and a system for screening for such genes

PRODUCTION OF CAROTENOIDS IN OLEAGINOUS YEAST AND FUNGI

... ²²²The present invention provides systems for producing engineered oleaginous ²yeast or fungi that express carotenoids.² ...

INCREASED CAROTENOID PRODUCTION BY INHIBITION OF SQUALENE BIOSYNTHESIS

The present invention relates to a biological process for producing carotenoids utilizing a microorganism which is capable of producing carotenoids and belonging to the genus Xanthophyllomyces (Phaffia) in the presence of an inhibitor for biosynthesis of sterols from farnesyl pyrophosphate.

PROCESS FOR THE ENANTIOSELECTIVE ENZYMATIC REDUCTION OF SECODIONE DERIVATIVES

The invention relates to a process for the enantioselective enzymatic reduction of secodione derivatives of general formula I (see formula I) wherein the ring structures comprise no, one or several heteroatoms, R1 is hydrogen or a C1-C4 alkyl group, R2 is hydrogen, a C1-C8 alkyl group or a protective group for OH known in prior art, such as an ester, R3 is hydrogen, a methyl group or a halide, the structural element (see above formula) represents a benzene ring or a C6 ring having 0, I or 2 C-C double bonds, a double bond is optionally included at positions 6/7 or 7/8, and the carbon at positions 1, 2, 4, 5, 6, 7, 8, 9, 11, 12 and 16 is independently substituted with hydrogen, a C1-C4 alkyl group, a halide or a phenyl group, wherein the secodione derivative is reduced with an oxidoreductase/ dehydrogenase in the presence of NADH or NADPH as a cofactor. According to the invention, the secodione derivative is used in the reaction batch at a concentration of >=10 g/l and the oxidized cofactor ...

YEAST CELLS, METHODS FOR THEIR PREPARATION, AND THEIR USE

A yeast cell, preferably a mutant of Phaffis rhodozyma, which, when grown under conditions comprising an oxygen transfer rate of at least 30 mmoles/1/hour on Difco YM medium at 20-22.degree.C for 5 days in 500 ml shake flasks with two baffles containing 50 ml of the medium and subjected to orbital shaking at 150 rpm, the inoculum being 100 .mu.l of a four days old YM culture, produces the red pigment, astaxanthin, in an amount of at least 300 .mu.g per g of yeast dry matter, typically at least 800 .mu.g per g of yeast dry matter, determined by HPLC analysis using pure astaxanthin as a standard on a methanol extract of the yeast prepared by subjecting a suspension of 0.2 g of yeast dry matter in 20 ml of methanol to 5 x 1 minutes of disintegration at intervals of half a minute, the disintegration being performed at a temperature of at the most 20.degree.C in a glass ball mill containing 15 g of glass balls having a diameter of 0.4 mm, the glass ball mill being provided with a cooling jacket ...

ACC GENE

The present invention relates to a gene useful in a process to increase the microbial production of carotenoids. The carotenoids astaxanthin is distributed in a wide variety of organisms such as animals, algae and microorganisms. It has a strong antioxidation property against reactive oxygen species. Astaxanthin is used as a coloring reagent, especially in the industry of farmed fish, such as salmon, because astaxanthin imparts distinctive orange- red coloration to the animals and contributes to consumer appeal in the marketplace.

GENES INVOLVED IN ISOPRENOID COMPOUND PRODUCTION

Genes have been isolated from Methylomonas 16a sp. encoding the isoprenoid biosynthetic pathway. The genes and gene products are the first isolated from a Methylomonas strain that is capable of utilizing single carbon (C1) substrates as energy sources. The genes and gene products of the present invention may be used in a variety of ways for the production of isoprenoid compounds in a variety of organisms.

FILAMENTOUS FUNGAL MUTANTS WITH IMPROVED HOMOLOGOUS RECOMBINATION EFFICIENCY

The present invention relates to a method for increasing the efficiency of targeted integration of a polynucleotide to a pre-determined site into the genome of a filamentous fungal cell with a preference for NHR, wherein said polynucleotide has a region of homology with said pre-determined site, comprising steering an integration pathway towards HR. The present invention also relates to a mutant filamentous fungus originating from a parent cell, said mutant having an HR pathway with elevated efficiency and/or an NHR pathway with a lowered efficiency and/or a NHR/HR ratio with decreased efficiency as compared to said HR and/or NHR efficiency and/or NHR/HR ratio of said parent cell under the same conditions.

PROCESS FOR PRODUCING CAROTENOIDS

The present invention relates to a biological process for producing carotenoids utilizing a microorganism which is capable of producing carotenoids and belonging to the genus Xanthophyllomyces (Phaffia) in the presence of an inhibitor for biosynthesis of sterols from farnesyl pyrophosphate.

SOLVENTLESS EXTRACTION PROCESS

PARALLEL CHROMOSOMAL STACKING OF TRAITS IN BACTERIA

The invention describes a method for the stacking of traits in a recombination proficient host using a phage transduction system. The method makes use of a nucleic acid integration cassette that has homology to a specific site on a host chromosome for the insertion of genetic elements and the stacking of traits. Repetition of the method results in the stacking of traits on a single genetic element.

CAROTENOIDS FOR TREATING OR PREVENTING NAUSEA

The present disclosure provides technologies relating to treatment of nausea and/or vomiting, and in some embodiments relates in particular to induced (e.g., chemotherapy-induced) nausea and/or vomiting. Alternatively, or additionally, in some embodiments, provides technologies relating to treatment or prevention of one or more feeding disorders (e.g., anorexia nervosa). Also provided herein are therapeutic compositions for oral delivery comprising a therapeutically effective amount of a C50 carotenoid compound, and a pharmaceutically acceptable carrier.

PRODUCTION OF ISOPRENOIDS

METHOD FOR PRODUCTION OF ASYMMETRIC CAROTENOIDS

Genes have been isolated from Rhodococcus and Deinococcus which encode a specific lycopene .beta.-cyclase capable of converting acyclic carotenoids with at least one .psi.-end group to the corresponding asymmetric carotenoid containing a single .beta.-ionone ring end group. The genes are new. Transformed host cells expressing the present genes and methods for the bio- conversion of acylic carotenoid substrates to corresponding asymmetric carotenoid are also provided.

METHOD FOR PRODUCING KETOCAROTINOIDS IN PLANT FRUIT

The invention relates to a method for producing ketocarotinoids by cultivating genetically modified plants which exhibit a ketolase activity in the fruit thereof.

PRODUCTION OF ISOPRENOID IN RECOMBINANT YEAST

... ²²²The invention provides a method of producing .alpha.-tocopherol and .alpha.-²tocopheryl esters and a method of producing vitamin A or .beta.-carotene. The ²methods comprise using a biological system to produce farnesol or ²geranylgeraniol. Then, the farnesol or geranylgeraniol is chemically converted ²into .alpha.-tocopherol, an .alpha.-tocopheryl ester, vitamin A or .beta. ²carotene.² ...

NOVEL MICROORGANISM AND METHOD FOR PRODUCING CAROTENOID USING THE SAME

It is intended to provide a bacterium selectively synthesizing canthaxanthin from which canthaxanthin, which is a carotenoid, can be simply extracted and purified and a method for producing canthaxanthin by a culture method. A carotenoid-producing bacterium belonging to the genus Paracoccus which selectively produces canthaxanthin which accounts for 90% by weight or more of the total amount of produced carotenoids which include .beta.-carotene, .bet a.- cryptoxanthin, echinenone, canthaxanthin, 3-hydroxyechinenone, 3~- hydroxyechinenone, zeaxanthin, phoenicoxanthin, adonixanthin and astaxanthin , and a method for producing canthaxanthin in which a cultivation is carried o ut using the bacterium and the carotenoids are collected from the bacterial cel l or the culture solution after the cultivation.

ENZYMATIC SYNTHESIS PROCESS FOR (7S)-3,4-DIMETHOXYBICYCLO(4.2.0)OCTA-1,3,5-TRIENE-7-CARBOXYLIC ACID OR OF ITS ESTERS AND APPLICATION OF IVABRADINE AND ITS SALTS TO THE SYNTHESIS

Procédé de synthèse enzymatique du composé de formule (I) : (voir formule I) dans laquelle R1 représente un atome d'hydrogène ou un groupement alkyle. Application à la synthèse de l'ivabradine et de ses sels d'addition à un acide pharmaceutiquement acceptable.

TREATMENT OF PHAFFIA RHODOZYMA

This invention relates to a process for enhancing the availability of astaxanthin in Phuffia rhodozyma yeast comprising forming an aqueous slurry by contacting the yeast with an aqueous solution of hydrochloric acid or sulfuric acid wherein the acid is present in an effective amount to modify the yeast's cell walls and thereafter heating the aqueous slurry at a temperature in the range of from about 60.degree.C to about 90.degree.C for about 2 hours to about 24 hours. The astaxanthin is useful as a dietary pigment supplement for salmonids and crustaceans grown on aquacultural farms.

ASTAXANTHIN-GENERATING YEAST CELLS

Yeast cells capable of generating at least 1100 .mu.g astaxanthin per gram and useful as a fish feed are disclosed. Mutagenisis of Phaffia rhodozyma using EMS and/or NTG is a preferred route to obtain these cells. Rupturing of the cells to release the pigment makes use of an alpha 1,3 glucanase containing enzyme. Methods of drying, concentrating and storing the cells are described.

PROCESS FOR PRODUCING ASTAXANTHIN IN PHAFFIA RHODOZYMA

The present invention provides novel strains of Phaffia rhodozyma, which produce high levels of astaxanthin a novel method for growing said Phaffia rhodozyma, and a novel method for providing the astaxanthin contained in Phaffia rhodozyma in a form suitable for use as A dietary pigment supplement.

PREPARATION OF MICROBIAL POLYUNSATURATED FATTY ACID CONTAINING OIL FROM PASTEURISED BIOMASS

The present invention discloses a microbial polyunsaturated fatty acid(PUFA)containing oil with a high triglyceride content and a high oxidative stability. In addition, a method is described for the recovery of such oil from a microbial biomass derived from a pasteurised fermentation broth, wherein the microbial biomass is subjected to extrusion to form granular particles, dried and the oil then extracted from the dried granules using an appropriate solvent.

IMPROVED METHODS FOR TRANSFORMING PHAFFIA STRAINS, TRANSFORMED PHAFFIA STRAINS SO OBTAINED AND RECOMBINANT DNA IN SAID METHODS

The present invention provides recombinant DNA comprising a transcription promoter and a downstream sequence to be expressed, in operable linkage therewith, wherein the transcription promoter comprises a region found upstream of the open reading frame of a highly expressed Phaffia gene, preferably a glycolytic pathway gene, more preferably the gene coding for Glyceraldehyde-3-Phosphate Dehydrogenase. Further preferred recombinant DNAs according to the invention contain promoters of ribosomal protein encoding genes, more preferably wherein the transcription promoter comprises a region found upstream of the open reading frame encoding a protein as represented by one of the amino acid sequences depicted in any one of SEQIDNOs: 24 to 50. According to a further aspect of the invention an isolated DNA sequence coding for an enzyme involved in the carotenoid biosynthetic pathway of Phaffia rhodozyma is provided, preferably wherein said enzyme has an activity selected from isopentenyl pyrophosphate ...

ASTAXANTHIN SYNTHETASE

The present invention is directed to genetic materials useful for the preparation of astaxanthin from beta-carotene, such as polypeptides having astaxanthin synthase activity, DNA fragments coding for astaxanthin synthase, recombinant organisms and the like. Those novel genetic materials may be originated from Phaffia rhodozyma. The present invention also provides a process for the production of astaxanthin.

Verfahren zur Herstellung von Lutein

Procédé de production de lycopène

Zeaxanthine prodn - by culture of flavobacter microorganism

In the title process a flavobacter producing the pigment is cultivated in a nutrient medium until an exponential growth rate is established and production of (I) commences, then the cells are aerobically cultivated at 22-5 degrees C in a medium contg. 15-35 mg/ml carbohydrate, a source of assimilable carbon, and at least one source of amino-N and the level of these ingredients is maintained at a constant level until a sufficient level of zeaxanthine is produced. In an example flavobacter ATCC No. 21.588 produces 20 mg/ml zeaxanthine after 50 hrs. compared with a yield of 12 mu g/ml by conventional means after 55 hrs.

Zeaxanthine prodn - by culture of flavobacter microorganism

In the title process a flavobacter producing the pigment is cultivated in a nutrient medium until an exponential growth rate is established and production of (I) commences, then the cells are aerobically cultivated at 22-5 degrees C in a medium contg. 15-35 mg/ml carbohydrate, a source of assimilable carbon, and at least one source of amino-N and the level of these ingredients is maintained at a constant level until a sufficient level of zeaxanthine is produced. In an example flavobacter ATCC No. 21.588 produces 20 mg/ml zeaxanthine after 50 hrs. compared with a yield of 12 mu g/ml by conventional means after 55 hrs.

VERFAHREN ZUR HERSTELLUNG VON ZEAXANTHIN.

Procédé de production de B-carotène

Procédé de production de B-carotène

Verfahren zur Herstellung von Lycopin und Verwendung desselben als Geflügelfutterzusatz

Procédé de préparation du B-carotène

Procédé de production de B-carotène par fermentation

Verfahren zur Herstellung eines bakteriellen Präparates mit einem hohen Gehalt an Oxycarotinoiden sowie eine Verwendung desselben

Procédé de production de B-carotène

Verfahren zur Herstellung von Lycopin

Verfahren zur Herstellung eines Mycels

Poultry food contg. zeaxanthin and vitamin=B12

Food for poultry is prepared by (a) culturing a variety of Flavobacter microorganism, known produce zeaxanthine, in an aq. medium contg. a carbohydrate as carbon source, a nitrogen source which is a free amino acid or its mineral salts, trace elements, and vitamins, together with 200-1000 ppm by wt., of an iron salt and 2-100 ppm, by wt. of a cobalt salt; and (b) recovering the cellular mass.The food contains zeaxanthine which improves the colour of the animals skin and egg yolks, and it also contains appreciable quantities of vitamin B12. Addition of the iron and cobalt salts doubles the quantity of B12 in the product. The medium pref. contains 0.1-15% sucrose or glucose, 0.1-8% yeast extract, corn steep liquor or protein hydrolysate, and 0.1-2% magnesium sulphate.

Biosynthesis of lycopene for use as food colourant

Biosynthesis of lycopene is effected by (a) treating a microorganism of the genus Flavobacter with 1-methyl-3-nitro-1-nitrosoguanidine (I) in a solidified nutrient medium contg. sucrose as C source to produce a lycopene-producing nutant; and (b) culturing the nutant in an aq. nutrient medium. The initial flavobacter strain is pref. ATCC 21588.Lycopene is a natural red pigment (e.g. occuring in ripe tomatoes) and is used as a food colouring. Cpd. (I) converts zeaxanthine-producing strains of Flavobacter (yellow colonies) into lycopene-producing nutants (red colonies).

Kosmetische Zusammensetzungen.

Die vorliegende Erfindung betrifft Zusammensetzungen, die Retinol, ein Öl und Carnosolsäure umfassen, wobei die Zusammensetzung die Oxidation von Retinol verringert oder verhindert und/oder die Oxidationsstabilität von Retinol erhöht. Die vorliegende Erfindung betrifft auch die Zusammensetzung umfassende Formulierungen sowie Anwendungen und Verfahren zur Verringerung oder Verhinderung der Oxidation von Retinol und/oder zur Erhöhung der Oxidationsstabilität von Retinol und ein Verfahren zur Herstellung solcher Zusammensetzungen.

APPLICATION OF THERMOPHILIC NUCLEASES FOR BREAKING NUCLEIC ACIDS

METHOD OF ISOLATING CAROTENOID FROM CAROTENOID - PRODUCING BIOORGANIZMA

karotingidroksilaza and its application for obtaining carotenoids

МУКОРОВЫЙ ГРИБ BLAKESLEA TRISPORA ШТАММ PHT 1+, PHT 1- - ПРОДУЦЕНТ ФИТОИНА

Селекционированно штамм Pht 1+; Pht 1- мукорового гриба Blakeslea trispora - продуцента фитоина. Штамм депонирован в депозитарии Института микробиологии и вирусологии им. Д.К. Заболотного НАН Украины под № F-100053. Концентрация фитоина зависит от соотношения двух половых форм и продолжительности ферментации. Оптимальным является соотношение 1:5 при 7-суточной ферментации. Выход фитоина составлял от 1,5 до 2,2 г/л культуральной жидкости или концентрации 10-15 %.

ШТАММ BLAKESLEA TRISPORA И СПОСОБ ЕГО ПРИМЕНЕНИЯ

Данное изобретение относится к штамму Blakeslea trispora (В. trispora) и его потомству, продуцирующему по крайней мере 0,3 г/л ликопина при совместном культивировании по крайней мере в течение 3 дней с приемлемым штаммом Blakeslea trispora противоположного типа спаривания в приемлемой среде при отсутствии экзогенного ингибитора каратиногенеза, к естественному, эффективному способу получения ликопина с использованием указанного штамма и к питательной среде, особенно пригодной для осуществления указанного способа.

A STRAIN (TKST) BLAKESLEA TRISPORA IMB F-100022 - PRODUCER OF --CAROTENE

A PROCESS FOR ISOLATION OF CAROTENOID CRYSTALS FROM MICROBIAL BIOMASS

STRAINS OF BLAKESLEA TRISPORA, PRODUCING LYCOPIN WITH HIGH YIELD ACCEPTABLE MEDIUM IN NO EXOGENOUS INHIBITOR KAROTINOGENEZA

Method for preparing a composition rich in lutein produced by microalgae

Application of gene YEL013W and gene YER042W

Algal culture production, harvesting, and processing

Materials and methods are provided for growing algae while maintaining culture selectivity. Algae that can be grown include, for example, green algae such as those of the genus Scenedesmus. Lipid obtained from the algae can be used to produce biofuels such as biodiesel or polyunsaturated fatty acids such as omega-3 fatty acids. Feedstocks such as animal feed and aquaculture feed can also be produced as can phytonutrients such as asataxanthin and beta-carotene.

Process of production of beta-carotene

Procede de production de carotenoides et notamment d'astaxanthine par culture de microalgues et dispositif pour la mise en oeuvre du procede

Ce procede consiste a cultiver la microalgue Haematococcus pluvialis dans un photoreacteur 4 alimente en continu 18, 12, 11, 6 en CO2 et en substrat nourricier 14, 11, 6 renfermant des nitrates, le rapport C/N dans le milieu de culture etant tel que 2 ¾ C/N ¾ 120 en fin de croissance de la microalgue, la concentration en C dans le milieu de culture allant de 0,22 a 12 mg/l et la concentration en N dans le milieu en fin de croissance etant voisine de 0 et en debut de croissance allant de 40 a 120 mg/l.

Process of production of beta-carotene by fermentation in the presence of heterocyclic hydrazides

Antioxidant and UV-protective composition useful in cosmetic or dermatological skin care preparations comprises the colorless carotenoid phytoene

La présente invention concerne une composition comprenant une quantité de phytoène efficace pour prévenir les dégâts provoqués par l'oxydation ou par l'exposition à la lumière UV. La composition est incolore et peut être utilisée pour réaliser des compositions cosmétiques ou pharmaceutiques aussi bien que pour des compositions alimentaires. La présente invention vise également un procédé de préparation de quantité suffisante de phytoène à partir d'organismes producteurs de caroténoïdes·.

PROCEEDED Of EXTRACTION INTENSIVE OF COMPOSE CELLULAR RESULTING FROM MICRO-ORGANISMS, BY SETTING IN CONTINUOUS CULTURE AND EXTRACTION, AND CORRESPONDING DEVICE.

Microorganism and method for producing canthaxanthin

A carotenoid producing bacterium belonging to the genus Paracoccus that selectively produces canthaxanthin so that the amount thereof is not less than 90 percent by weight of the total amount of produced carotenoids including β-carotene, β-cryptoxanthin, echinenone, canthaxanthin, 3-hydroxyechinenone, 3′-hydroxyechinenone, zeaxanthin, phoenicoxanthin, adonixanthin, and astaxanthin. A method for producing canthaxanthin by culturing the above bacterium, and then collecting carotenoids from bacterial cells or a culture solution after the culturing.

Isolation of chlorophylls from intact algal cells

A method for isolating chlorophylls from intact algal cells is provided. The method includes dewatering intact algal cells from an algal cell culture to make an algal biomass, extracting lipids along with carotenoids and chlorophylls from the algal biomass, and separation of the chlorophylls using adsorption or membrane diafiltration or other methods. The method can include selective extraction of other algal components including polar lipids, neutral lipids, proteins, and carbohydrates. The method may include esterifying the lipids with a catalyst in the presence of an alcohol, and separating a water soluble fraction comprising glycerin from a water insoluble fraction comprising fuel esters and distilling the fuel esters under vacuum to obtain a C16 or shorter fuel esters fraction, a C16 or longer fuel ester fraction, and a residue comprising omega-3 fatty acids esters and remaining carotenoids.

Production of carotenoids in oleaginous yeast and fungi

The present invention provides systems for producing engineered oleaginous yeast or fungi that express carotenoids

METHODS AND STRAINS FOR THE PRODUCTION OF SARCINAXANTHIN AND DERIVATIVES THEREOF

The present invention relates to a new strain of , named Otnes7, which is superior to known strains in its ability to synthesise the carotenoid sarcinaxanthin and a method of producing sarcinaxanthin or a derivative thereof, said method comprising introducing into and expressing in a host cell one or more nucleic acid molecules encoding an activity in the sarcinaxanthin biosynthetic pathway. 1. A method of producing sarcinaxanthin or a derivative thereof , said method comprising introducing into and expressing in a host cell one or more nucleic acid molecules encoding an activity in the sarcinaxanthin biosynthetic pathway , wherein said one or more nucleic acid molecules comprise:(i) a nucleotide sequence as set forth in SEQ ID NO: 37 or a part thereof;(ii) a nucleotide sequence with at least 90% sequence identity to SEQ ID NO: 37, or a part thereof; or(iii) a nucleotide sequence complementary to (i) or (ii).2. The method of claim 1 , wherein said one or more nucleic acid molecules comprise:(i) a nucleotide sequence as set forth in SEQ ID NO: 26 or a part thereof;(ii) a nucleotide sequence with at least 90% sequence identity to SEQ ID NO: 26, or a part thereof; or(iii) a nucleotide sequence complementary to (i) or (ii).3. The method of claim 1 , wherein said one or more nucleic acid molecules encode the sarcinaxanthin biosynthetic pathway.4. The method of claim 1 , further comprising the step of isolating the sarcinaxanthin or derivative thereof from the host cell.6. The method of claim 5 , wherein said host cell is a lycopene-producing host cell claim 5 , preferably wherein said lycopene-producing host cell is capable of producing lycopene at levels of at least 0.5 mg/g CDW claim 5 , further preferably claim 5 , wherein the lycopene producing host cell comprises the plasmid pAC-LYC.7. The method of claim 6 , wherein said one or more proteins of (a) are capable of catalysing the conversion of lycopene to flavuxanthin.8. The method of claim 7 , wherein said one or ...

METHOD OF PRODUCING ISOPRENOID COMPOUNDS IN YEAST

Yeast strains capable of increased prenyl phosphate production are provided, enabling increased terpenoid molecule production. Heterologous yeast strains with high prenyl phosphate availability are prepared using one or both of two different strategies for increasing the availability of prenyl phosphates for terpenoid production. First, by co-expressing multiple mevalonate pathway gene analogs, a novel heterologous combination of genes results, some of which increases the inherent availability of prenyl phosphates in yeast. Second, by expressing the non-endogenous enzyme ATP citrate lysase (ACL), a buildup of high cytosolic concentration of acetyl-CoA is produced in the cytosol of 1. A method of producing isoprenoid compounds in a yeast cell , the method comprising cultivating a yeast cell in a suitable medium , the yeast cell comprisinga) one or more heterologous nucleotide sequences encoding MEV-1 (SEQ ID. NO. 36), MEV-6 (SEQ ID. NO. 41), MEV-15 (SEQ ID. NO. 50), MEV-18 (SEQ ID. NO. 53), MEV-21 (SEQ ID. NO. 56), or MEV-23 (SEQ ID. NO. 58); and/orb) reduced inherent ACO1 expression relative to an unaltered yeast cell.24-. (canceled)5. A method for making a yeast host cell with increased synthesis of isoprenoid compounds relative to an unaltered yeast cell , the method comprisinga) introducing one or more heterologous nucleotide sequences encoding MEV-1 (SEQ ID. NO. 36), MEV-6 (SEQ ID. NO. 41), MEV-15 (SEQ ID. NO. 50), MEV-18 (SEQ ID. NO. 53), MEV-21 (SEQ ID. NO. 56), or MEV-23 (SEQ ID. NO. 58) and/orb) reducing inherent ACO1 expression relative to an unaltered yeast cell.6. A yeast host cell comprising a) one or more heterologous nucleotide sequences encoding MEV-1 (SEQ ID. NO. 36) , MEV-6 (SEQ ID. NO. 41) , MEV-15 (SEQ ID. NO. 50) , MEV-18 (SEQ ID. NO. 53) , MEV-21 (SEQ ID. NO. 56) , or MEV-23 (SEQ ID. NO. 58); and/orb) reduced inherent ACO1 expression relative to an unaltered yeast cell.7. The method of claim 1 , wherein the yeast cell further comprises one or ...

HETEROTROPHIC MICROBIAL PRODUCTION OF XANTHOPHYLL PIGMENTS

The invention relates to a microbial biomass wherein the biomass comprises at least one xanthophyll selected from any one or more of fucoxanthin, diatoxanthin and diadinoxanthin. The invention further relates to a process for producing such a microbial biomass. Further the invention relates to compositions comprising at least one xanthophyll selected from any one or more of fucoxanthin, diatoxanthin and diadinoxanthin and processes for producing such compositions. 1. A microbial biomass , produced from a heterotrophic fermentation , the biomass comprising at least one xanthophyll selected from any one or more of fucoxanthin , diatoxanthin and diadinoxanthin.2. The microbial biomass of wherein the xanthophyll is fucoxanthin.35-. (canceled)6. The microbial biomass of wherein the xanthophyll is present at levels equal to or greater than about 0.1% of dry cell weight of the biomass.79-. (canceled)10. The microbial biomass of wherein the microbial biomass is a marine diatom biomass.11. (canceled)12. A process for producing a microbial biomass claim 1 , wherein the microbial biomass comprises at least one xanthophyll selected from any one or more of fucoxanthin claim 1 , diatoxanthin and diadinoxanthin claim 1 , the process comprising the steps of:cultivating a microorganism in heterotrophic culture to produce the biomass; andrecovering said biomass.13. The process of wherein the xanthophyll is fucoxanthin.1415-. (canceled)16. The process of wherein the xanthophyll is present at levels equal to or greater than about 0.1% of dry cell weight of the biomass.1719-. (canceled)20. The process of wherein the microbial biomass is a marine diatom biomass.21. (canceled)22. The process of wherein at least about 1 mg of the xanthophyll is produced per litre of culture per hour.23. (canceled)24. The process of wherein the step of cultivating the microorganism in heterotrophic culture comprises a culture phase in which cells are grown under conditions in which organic carbon is used as ...

Method and apparatus for producing cells and fat soluble materials by cell culture

The present invention relates to a method and apparatus for producing cells without injury and fat-soluble materials by from cell culturing in an inexpensive and highly efficient manner. The apparatus according to the present invention comprises a culturing device 10 , a solvent device 20 , a mixing device 30 , a separation device 40 , a fractionation device 50 , a cell accommodation device 60 , and a fat-soluble material solvent accommodation device 70.

INTEGRATED CARBON CAPTURE AND ALGAE CULTURE

The feasibility of using COfrom a concentrated source to grow microalgae is limited by the high cost of COcapture and transportation, as well as significant COloss during algae culture. Another challenge is the inability of algae in using COduring night while COis continuously produced from the source. To address these challenges, this invention provides a process in which COis captured as bicarbonate and used as feedstock for algae culture. Then the carbonate is regenerated in the algae culture process as absorbent to capture more CO, which is converted to bicarbonate for use as feedstock, etc. This process significantly reduces carbon capture costs since it avoids the energy for carbonate regeneration. Also, transporting a solid or aqueous bicarbonate solution has a much lower cost than transporting compressed CO, and using bicarbonate provides a better alternative for COdelivery to algae culture systems than supplying COgas. 1. An integrated method culturing algae or cyanobacteria , comprising the steps of{'sub': 2', '2, 'i) capturing COfrom a source of CO;'}{'sub': '2', 'ii) converting captured COinto bicarbonate;'}iii) culturing alkaliphilic algae or alkaliphilic cyanobacteria using said bicarbonate as a carbon source to produce algal bioproducts;{'sub': '2', 'iv) using spent medium from said step of culturing as said source of COin said step of capturing; and'}v) repeating steps i) to iv).2. The method of claim 1 , wherein said bicarbonate is in a form selected from the group consisting of solid bicarbonate and a liquid bicarbonate solution.3SynechocystisCyanotheceMicrocoleusEuhalotheceSpirulina. The method of claim 1 , wherein said alkaliphilic cyanobacteria are selected from the group consisting of sp. claim 1 , sp. claim 1 , sp. claim 1 , sp. and sp.4ChlorellaDunaliella.. The method of claim 1 , wherein said alkaliphilic algae are eukaryotic microalgae selected from the group consisting of and5. The method of claim 1 , wherein culture medium used in said ...

METHODS FOR ISOLATING BACTERIA

The present invention relates to compositions and methods to identify novel bacteria and metabolites derived therefrom. More specifically, the invention describes a novel method to isolate bacteria producing metabolites of interest from environmental samples. Particularly, the invention discloses a method to select rare antibiotic producing bacteria. The invention can be used from any sample and allows the isolation of bacteria having e.g., pharmaceutical or agrochemical interest. 1Deinococcus. A method for producing a drug , comprising (i) culturing , in a suitable medium , a bacterium which naturally produces said drug or an intermediate thereof , (ii) collecting or purifying the drug or intermediate from the culture , and (iii) when an intermediate is produced in step (i) , converting said intermediate into said drug , wherein said bacterium is a or a related bacterium.2Deinococcus. An improved method for the production of a drug from a cultured microbial cell , the improvement consisting in the use , as said microbial cell , of a or related bacterium.3Deinococcus bacterium.. The method of claim 1 , wherein the bacterium is a4Deinococcus bacterium.. The method of claim 1 , wherein the bacterium is a wild-type5Deinococcus bacterium.. The method of claim 1 , wherein the bacterium is a recombinant6. The method of claim 1 , wherein the bacterium is cultured in a defined medium.7. The method of claim 1 , wherein the bacterium is cultured in a complex medium.8. The method of claim 1 , wherein the bacterium is cultured at a pH comprised between 4 and 8.9. The method of claim 1 , wherein the bacterium is cultured in a fermentation device or as a continuous culture.10. The method of claim 1 , wherein the drug is selected from an antibiotic claim 1 , a bacteriostatic agent claim 1 , an anti-parasitic agent claim 1 , an anti-fungal agent claim 1 , an anti-viral agent claim 1 , an anti-metabolite agent claim 1 , a chemotherapeutic agent claim 1 , a cytokine claim 1 , a cell- ...

CELLS AND METHODS FOR PRODUCING LUTEIN

Provided herein are recombinant cells (e.g., recombinant bacteria or plant, insect, mammalian, and yeast cells) containing a nucleic acid encoding a CYP97A protein or a nucleic acid encoding a CYP97B protein; a nucleic acid encoding a CYP97C protein; a nucleic acid encoding a geranylgeranyl pyrophosphate synthase protein; a nucleic acid encoding a phytoene synthase protein; a nucleic acid encoding a phytoene desaturase protein; a nucleic acid encoding a lycopene β-cyclase protein; and a nucleic acid encoding a lycopene ε-cyclase protein. Also provided are methods of producing lutein that include culturing these recombinant cells (e.g., recombinant bacteria and yeast cells), and methods of generating these recombinant cells (e.g., recombinant bacteria and yeast cells). Also provided is lutein produced by these methods, and pharmaceutical compositions, food supplements, food products, and cosmetic compositions that contain lutein produced by these methods. 1. A recombinant bacterium or yeast cell comprising:a nucleic acid encoding a CYP97A protein or a nucleic acid encoding a CYP97B protein;a nucleic acid encoding a CYP97C protein;a nucleic acid encoding a geranylgeranyl pyrophosphate synthase protein;a nucleic acid encoding a phytoene synthase protein;a nucleic acid encoding a phytoene desaturase protein;a nucleic acid encoding a lycopene β-cyclase protein; anda nucleic acid encoding a lycopene ε-cyclase protein.2. The recombinant bacterium or yeast cell of claim 1 , further comprising:a nucleic acid encoding a D-1-deoxyxylulose 5-phosphate synthase protein; and/ora nucleic acid encoding an isopentenyl pyrophosphate isomerase protein.3. The recombinant bacterium or yeast cell of claim 1 , wherein the bacterium or yeast cell comprises a nucleic acid encoding a CYP97A protein.4. The recombinant bacterium or yeast cell of claim 3 , wherein the CYP97A protein comprises a sequence at least 80% identical to SEQ ID NO: 1.5. The recombinant bacterium or yeast cell of claim 1 ...

A CRISPR-CAS SYSTEM FOR A YEAST HOST CELL

The present invention relates to the field of molecular biology and cell biology. More specifically, the present invention relates to a CRISPR-CAS system for a yeast host cell. 1SaccharomycesKluyveromyces. A non-naturally occurring or engineered composition comprising a source of a CRISPR-Cas system comprising a guide-polynucleotide and a Cas protein , wherein the guide-polynucleotide comprises a guide-sequence that essentially is the reverse complement of a target-polynucleotide in a host cell and the guide-polynucleotide can direct binding of the Cas protein at the target-polynucleotide in the host cell to form a CRISPR-Cas complex , wherein the guide-sequence is essentially the reverse complement of the (N)y part of a 5′-(N)yPAM-3′ polynucleotide sequence target in the genome of the host cell , wherein y is an integer of 8-30 , wherein PAM is a protospacer adjacent motif , wherein the host cell is a eukaryote , which eukaryote is a yeast , optionally a or a and wherein PAM is optionally a sequence selected from the group consisting of 5′-XGG-3′ , 5′-XGGXG-3′ , 5′-XXAGAAW-3′ , 5′-XXXXGATT-3′ , 5′-XXAGAA-3′ , 5′-XAAAAC-3′ , wherein X can be any nucleotide or analog thereof , optionally X can be any nucleotide; and W is A or T.2. A composition according to claim 1 , wherein the Cas protein is encoded by a polynucleotide and/or the guide-polynucleotide is encoded by or present on a polynucleotide.3. A composition according to claim 1 , wherein the Cas protein is encoded by a polynucleotide and/or the guide-polynucleotide is encoded by or present on another polynucleotide and the polynucleotide or polynucleotides are comprised in a vector.4. A composition according to claim 1 , wherein the guide polynucleotide is encoded by a polynucleotide that is transcribed to provide for the actual guide-polynucleotide.5. A composition according to claim 1 , wherein a polynucleotide encoding a guide-polynucleotide has sequence identity with a vector such that recombination of the ...

METHOD FOR PRODUCING ASTAXANTHIN

Efficiency of production method for producing astaxanthin by culturing microalgae is improved. A method for producing astaxanthin in which astaxanthin is produced in inner of algae by culturing a microalga, wherein a light irradiation during a green stage culturing of the microalga is performed by using both a blue LED of peak wavelength from 420 to 500 nm and a red LED of peak wavelength from 620 to 690 nm, and having a ratio of photon flux density of the blue LED to the red LED to be 2:3 to 20:1. It is preferable that the photon flux density of the blue LED is from 5 to 200 μmol/m/s, and the photon flux density of the red LED is from 5 to 200 μmol/m/s. 1. A method for producing astaxanthin in which astaxanthin is produced in inner of algae by culturing a microalgae , wherein a light irradiation during a green stage culturing of the microalgae is performed by using both a blue LED of peak wavelength from 420 to 500 nm and a red LED of peak wavelength from 620 to 690 nm , and having a ratio of photon flux density of the blue LED to the red LED to be 2:3 to 20:1.2. The method for producing astaxanthin according to claim 1 , wherein the ratio of photon flux density of the blue LED to the red LED is 3:2 to 20:1.3. The method for producing astaxanthin according to claim 1 , wherein the light irradiation using the blue LED and the red LED is continuously performed during the green stage culturing.4. The method for producing astaxanthin according to claim 1 , wherein the photon flux density of the blue LED is from 5 to 200 μmol/m/s claim 1 , and the photon flux density of the red LED is from 5 to 200 μmol/m/s.5. The method for producing astaxanthin according to claim 1 , wherein a light irradiation during a red stage culturing of the microalgae is performed further using both the blue LED of peak wavelength from 420 to 500 nm and the red LED of peak wavelength from 620 to 690 nm.6. The method for producing astaxanthin according to claim 5 , wherein the ratio of photon ...

MICROBIAL ENGINEERING FOR THE PRODUCTION OF CHEMICAL AND PHARMACEUTICAL PRODUCTS FROM THE ISOPRENOID PATHWAY

The invention relates to the production of one or more terpenoids through microbial engineering, and relates to the manufacture of products comprising terpenoids. 1207.-. (canceled)208. A method for making a product containing limonene , or derivative thereof , the method comprising:{'i': Escherichia coli', 'E. coli, 'providing an () that produces isopentyl pyrophosphate (IPP) and dimethylallyl pyrophosphate (DMAPP) through an upstream methylerythritol (MEP) pathway and converts the IPP and DMAPP to limonene, or derivative thereof, through a recombinantly expressed downstream terpenoid synthesis pathway comprising geranyl pyrophosphate synthase and limonene synthase;'}{'i': 'E. coli', 'culturing the to produce the limonene, or derivative thereof, wherein the accumulation of indole in the culture is controlled to below 100 mg/L to thereby increase production of limonene, or derivative thereof; and'}incorporating the limonene, or derivative thereof, into a product.209. The method of claim 208 , wherein the product is a food product claim 208 , food additive claim 208 , beverage claim 208 , chewing gum claim 208 , candy claim 208 , or oral care product.210. The method of claim 208 , wherein the product is a fragrance product claim 208 , a cosmetic claim 208 , a cleaning product claim 208 , or a soap.211. The method of claim 208 , wherein the product is an insecticide claim 208 , pesticide claim 208 , or pest control agent.212. The method of claim 208 , wherein accumulation of indole in the culture is controlled by balancing the upstream MEP pathway with the downstream terpenoid synthesis pathway.213. The method of claim 208 , further comprising measuring the amount or concentration of indole continuously or intermittently.214. The method of claim 208 , wherein accumulation of indole in the culture is maintained to below 50 mg/L.215. The method of claim 208 , wherein accumulation of indole in the culture is maintained to below 10 mg/L.216. The method of claim 208 , ...

COMPOSITIONS AND METHODS OF BIOSYNTHESIZING CAROTENOIDS AND THEIR DERIVATIVES

The present invention relates to compositions and methods of producing carotenoids and carotenoid derivatives. 1. A recombinant microorganism comprising at least one nucleic acid construct comprising:a) a nucleic acid sequence encoding a lycopene β-cyclase enzyme; andb) a nucleic acid sequence encoding a carotenoid cleavage dioxygenase enzyme;wherein the nucleic acid sequences are operably linked to one or more expression control sequence, and wherein the microorganism further comprises lycopene.2Mucor circinelloidesPhycomyces blakesleeanusErwinia herbicola.. The microorganism of claim 1 , wherein the lycopene β-cyclase is selected from a lycopene cyclase enzyme of a bifunctional lycopene cyclase/phytoene synthase encoded by carRP of claim 1 , lycopene cyclase/phytoene synthase encoded by carRA of claim 1 , and lycopene cyclase encoded by crtY from3Daucus carota.. The microorganism of claim 1 , wherein the carotenoid cleavage dioxygenase enzyme is encoded by CCD1 from4. The microorganism of claim 1 , wherein the microorganism is selected from a microorganism genetically engineered to inhibit the expression of lycopene α-cyclase and a microorganism naturally not capable of expressing lycopene α-cyclase.5Yarrowia lipolytica, Saccharomyces cerevisiaeE. coli.. The microorganism of claim 1 , wherein the microorganism is selected from claim 1 , and6. The microorganism of claim 1 , comprising β-ionone.7. The recombinant microorganism of claim 1 , wherein the nucleic acid expression construct comprises a nucleic acid sequence encoding a carotenoid cleavage dioxygenase enzyme with at least 80% identity to an amino acid sequence of SEQ ID NO: 88.8. The recombinant microorganism of claim 1 , wherein the nucleic acid expression construct comprises a nucleic acid sequence encoding a lycopene β-cyclase enzyme with at least 80% identity to an amino acid sequence encoded by a nucleic acid sequence selected from SEQ ID NO: 64 and SEQ ID NO: 67.9. A recombinant microorganism ...

METHOD FOR PRODUCING ASTAXANTHIN BY FERMENTATION

An object of the present invention is to provide a method for microbioiogically producing astaxanthin of high concentration at low cost while suppressing production of canthaxanthin. Specifically, the present invention relates to a method for producing carotenoids including astaxanthin comprising culturing a bacterium that concurrently produces astaxanthin and canthaxanthin in a medium containing biotin, wherein a ratio of concentration of produced canthaxanthin to concentration of produced astaxanthin in a culture product after the end of culture in the medium is lower than that in a culture product alter the end of culture in a biotin-free medium. 1. A method for producing carotenoids comprising:{'i': 'Paracoccus', '1) culturing a bacterial strain in a culture medium containing biotin at a concentration of 0.02 mg/L to 50 mg/L by addition of biotin to the culture medium, wherein the bacterial strain is a member of the genus and concurrently produces astaxanthin and canthaxanthin;'}2) controlling the concentration of dissolved oxygen in the culture medium at 2 ppm or more during the intermediate phase of culture, thereby producing a carotenoid-containing culture broth in which the ratio or the concentration or canthaxanthin to the concentration of astaxanthin in the culture broth at the end of the culture period is 8% by mass or loss; and3) collecting the carotenoids from the carotenoid-containing culture broth.2. The method of claim further comprising increasing the concentration of dissolved oxygen in the culture medium in a stepwise or continuous manner during the intermediate phase of culture , wherein the concentration of dissolved oxygen in the culture medium at the beginning of the intermediate phase of culture before increasing the concentration is 2 ppm to 3.5 ppm.3. The method of claim 1 , wherein the concentration of gluconic acid in the carotenoid-containing culture broth at the end of the culture period is 30 g/L or less.4. The method of claim 1 , ...

GENETICALLY MODIFIED HOST CELLS AND USE OF SAME FOR PRODUCING ISOPRENOID COMPOUNDS

The present invention provides genetically modified eukaryotic host cells that produce isoprenoid precursors or isoprenoid compounds. A subject genetically modified host cell comprises increased activity levels of one or more of mevalonate pathway enzymes, increased levels of prenyltransferase activity, and decreased levels of squalene synthase activity. Methods are provided for the production of an isoprenoid compound or an isoprenoid precursor in a subject genetically modified eukaryotic host cell. The methods generally involve culturing a subject genetically modified host cell under conditions that promote production of high levels of an isoprenoid or isoprenoid precursor compound. 1. A genetically modified eukaryotic host cell that produces an isoprenoid or an isoprenoid precursor compound via a mevalonate pathway , the genetically modified eukaryotic host cell comprising genetic modifications that provide for:a) an increased level of activity of one or more mevalonate pathway enzymes,b) an increased level of prenyltransferase activity, andc) a decreased level of squalene synthase activitywherein the genetic modifications provide for production of an isoprenoid or an isoprenoid precursor compound at a level that is at least about 50% higher than the level of the isoprenoid or isoprenoid precursor compound in a control cell not comprising the genetic modifications.2. The genetically modified eukaryotic host cell of claim 1 , wherein the prenyltransferase is farnesyl pyrophosphate synthase.3. The genetically modified eukaryotic host cell of claim 1 , wherein the prenyltransferase is geranyl pyrophosphate synthase.4. The genetically modified eukaryotic host cell of claim 1 , wherein the prenyltransferase is geranylgeranyl pyrophosphate synthase.5. The genetically modified eukaryotic host cell of claim 1 , wherein the genetically modified eukaryotic host cell is a yeast cell.6Saccharomyces cerevisiae.. The genetically modified host cell of claim 5 , wherein the ...

CO-PRODUCTION OF A SESQUITERPENE AND A CAROTENOID

Provided herein are compositions and methods for co-production and recovery of two or more isoprenoids from a single recombinant cell. 1. A method for co-production of two or more isoprenoids , the method comprising:(a) culturing, in a culture medium, a host cell genetically modified with one or more heterologous nucleic acids encoding one or more enzymes in a first biosynthetic pathway to produce a first isoprenoid and with one or more heterologous nucleic acid encoding one or more enzymes in a second biosynthetic pathway to produce a second isoprenoid, which has a molecular weight that is different from the first isoprenoid; and(b) recovering the first isoprenoid; and(c) recovering the second isoprenoid.2. The method of wherein the host cell is not genetically modified to produce a target compound for recovery other than a compound derived from IPP.3. The method for co-production of isoprenoids of claim 1 , wherein the first isoprenoid and second isoprenoid are produced concurrently during a fermentation run from a single inoculum.4. The method for co-production of isoprenoids of claim 1 , wherein the first isoprenoid and second isoprenoid are produced sequentially from a single inoculum comprising the host cell claim 1 , or sequentially using a genetic switch.5. (canceled)6. The method for co-production of isoprenoids of claim 1 , wherein the culturing and recovering comprise:(a) culturing the single inoculum comprising the host cell to build a population of host cells;(b) culturing the population of host cells under conditions to produce the first isoprenoid from the population of host cells, wherein the conditions do not activate production of the second isoprenoid;(c) recovering the first isoprenoid from the population;(d) after separating the first isoprenoid, culturing the population or a subpopulation of the host cells under conditions to activate production of the second isoprenoid; and(e) recovering the second isoprenoid.7. The method for co-production of ...

PRODUCTION OF ASTAXANTHIN AND DOCOSAHEXAENOIC ACID IN MIXOTROPHIC MODE USING SCHIZOCHYTRIUM

New strains of protists belonging to the genus, allow high-yield production of lipids and carotenoids, in particular of astaxanthin and docosahexaenoic acid (DHA), in mixotrophic mode, and a method for selecting and culturing such strains, using a variable and/or discontinuous light source, in particular a flashing light. 1. Method comprising the following step:{'i': 'Schizochytrium', 'sup': −2', '−1', '−2', '−1, 'a) culture, in mixotrophic mode, of one or more strains of the genus under conditions of illumination that is discontinuous and/or variable over time, the illumination having variations in intensity, the amplitude of which is comprised between 5 μmol·m·sand 1,000 μmol·m·s, these variations taking place between 2 and 3,600 times per hour.'}2. Method according to claim 1 , characterized in that the illumination has variations in intensity claim 1 , the amplitude of which is comprised between 5 μmol·m·sand 400 μmol·m claim 1 , these variations taking place between 2 and 200 times per hour.3. Method according to claim 1 , characterized in that the culture is carried out in the presence of an organic carbon-containing substrate at a concentration from 100 mM to 1.5 M claim 1 , preferably from 300 mM to 1.2 M claim 1 , more preferentially from 500 mM to 1 M claim 1 , and even more preferentially from 600 mM to 900 mM claim 1 , the organic carbon-containing substrate being selected from saccharose claim 1 , glycerol claim 1 , glucose and cellulose derivatives and a mixture of these molecules.4. Method according to claim 3 , characterized in that said organic carbon-containing substrate present in the culture medium comprises at least 300 mM of glucose and/or glycerol.5. Method according to claim 1 , characterized in that the amplitude of the variations in intensity is comprised between 5 and 1 claim 1 ,000 μmol·m·s claim 1 , preferably between 70 and 300 μmol·m·s claim 1 , and more preferentially between 100 and 200 μmol·m·s.6. Method according to claim 1 , ...

PRODUCTION OF LUTEIN IN MIXOTROPHIC MODE BY SCENEDESMUS

Novel strains of microalgae belonging to the genus enable the production of lipids, in particular lutein, in mixotrophic mode, as well as a method for selecting and culturing the strains using a variable and/or discontinuous supply of light, in particular in the form of flashes. 1. Method comprising the following step:{'i': 'Scenedesmus', 'sup': −2', '−1', '−2', '−1, 'a) culture, in mixotrophic mode, of one or more strains of the genus under conditions of illumination that is discontinuous and/or variable over time, the illumination having variations in intensity, the amplitude of which is comprised between 5 and 1,000, 5 μmol·m·sand 400 μmol·m·s, these variations taking place between 2 and 3,600 times per hour.'}2. Method according to claim 1 , characterized in that the illumination has variations in intensity claim 1 , the amplitude of which is comprised between 5 μmol·m·sand 400 μmol·m claim 1 , these variations taking place between 2 and 200 times per hour.3Scenedesmus. Method according to claim 1 , characterized in that the microalga is from the species sp.4. Method according to claim 1 , characterized in that the culture is carried out in the presence of an organic carbon-containing substrate at a concentration from 5 mM to 1 M claim 1 , preferably from 50 mM to 800 mM claim 1 , more preferentially from 70 mM to 600 mM claim 1 , and even more preferentially from 100 mM to 500 mM claim 1 , the organic carbon-containing substrate being selected from starch claim 1 , lactate claim 1 , lactose claim 1 , saccharose claim 1 , acetate claim 1 , glycerol claim 1 , glucose and cellulose derivatives and a mixture of these molecules.5. Method according to claim 4 , characterized in that said organic carbon-containing substrate present in the culture medium comprises at least 5 mM of glucose.6. Method according to claim 5 , characterized in that the amplitude of the variations in intensity is comprised between 5 and 1 claim 5 ,000 claim 5 , preferably between 30 and 400 μ ...

METHOD FOR STABILISING OXIDATION-SENSITIVE METABOLITES PRODUCED BY MICROALGAE OF THE CHLORELLA GENUS

The invention relates to a method for stabilising a biomass of microalgae containing oxidation-sensitive metabolites selected from the group consisting of carotenoids (lutein, etc.), monounsaturated and polyunsaturated fatty acids (palmitoleic acid, oleic acid, linoleic acid, etc.), chlorophyll pigments (chlorophyll A and B, etc.) and vitamins (vitamin B9 and B12, etc.) taken individually or together, more specifically carotenoids, said method comprising the fermentation of said biomass in heterotrophic conditions. 113-. (canceled)14. A method for stabilizing or for storing oxidation-sensitive metabolites selected from the group consisting of the carotenoids , monounsaturated and polyunsaturated fatty acids , chlorophyll pigments , and vitamins , alone or in combination comprising:fermenting a biomass of microalgae in heterotrophic conditions comprising a culture phase deficient in a nutrient factor; andstoring the dry biomass in which the oxidation-sensitive metabolites are stabilized.15Chlorella.. The method of claim 14 , wherein the microalgae are of the genus16Chlorella sorokiniana.. The method of claim 15 , wherein the microalgae are17. The method of claim 15 , wherein the method does not comprise adding exogenous antioxidant or stabilizer to said dry biomass.18. The method of claim 15 , wherein the deficient nutrient factor is the carbon-containing source.19. The method of claim 18 , wherein the fermenting of said biomass of microalgae in heterotrophic conditions comprises:a first step of fermentation in batch mode,a second step of fermentation in fed-batch mode which, when the carbon-containing source is completely consumed by the microalgae, involves continuous supply of said carbon-containing source at a rate lower than its rate of consumption by the microalgae.20. The method of claim 18 , wherein the deficient nutrient factor is glucose and in that it is supplied to the culture at a rate above 1 g/l/h.21. The method of claim 19 , wherein the deficient ...

COMPOSITION AND METHOD FOR ENHANCING PHOTOSYNTHETIC EFFICIENCY OF MICROORGANISMS

Compositions including metal nano- and/or micro-particles in solution with photosynthetic bioproduct producing microorganisms. These light harvesting complexes increase growth rates and photosynthetic efficiency of the constituent microorganisms, reducing the light required for a specific production level, or increases production for a specific light level. 1. A composition comprising a nano- and/or micro-particle complexed to a photosynthetic microorganism.2. A composition according to claim 1 , wherein said the nano- and/or micro-particles of said complex are metal particles selected from the group consisting of gold claim 1 , silver claim 1 , copper claim 1 , and silicon claim 1 , and wherein the photosynthetic microorganism of the complex is selected from the group consisting of bacteria claim 1 , algae claim 1 , chlorophyte claim 1 , protists and fungi.3F. diplosiphon, NostocTolypothrixCalothrixSynechococcus elongatus, SynechocystisArthrospiraAphanotheceAnabaena. A composition according to claim 2 , comprising a photosynthetic bacteria selected from the group consisting of sp. claim 2 , sp. claim 2 , sp. claim 2 , sp. PCC6803 claim 2 , sp claim 2 , sp and sp.4F. diplosiphon.. A composition according to claim 3 , wherein the photosynthetic bacteria is5F. diplosiphon. A composition according to claim 4 , wherein the has increased halotolerance relative to wild type strains6. A composition according to claim 2 , wherein the particle is a gold nano-particle.7. A composition according to claim 4 , wherein the particle is a gold nano-particle.8ChlorellaNannochlorisDunaliella tertiolecta.. A composition according to claim 2 , comprising a photosynthetic algae selected from the group consisting of diatoms claim 2 , sp. claim 2 , sp. claim 2 , and9. A composition according to claim 2 , wherein the microorganism is suitable for use as a biofuel.10. A composition according to claim 2 , wherein the microorganism is a bioproduct producing microorganism.11. A composition ...

RECOMBINANT MICROORGANISM FOR PREPARING TERPENOID AND METHOD FOR CONSTRUCTING RECOMBINANT MICROORGANISM

Provided are a recombinant strain for preparing a terpenoid, and method for constructing the recombinant strain. Also provided is a recombinant bacterium 1, the recombinant bacterium 1 being a recombinant bacterium obtained in order to improve the enzymatic activity of α-ketoglutarate dehydrogenase in or the mutant thereof. The method for improving the enzymatic activity of α-ketoglutarate dehydrogenase in or the mutant thereof is replacing the original regulating element of the ketoglutarate dehydrogenase gene (sucAB) in or the mutant thereof with any of the following regulating elements: artificial regulating element M1-46, M1-37, and M1-93. Also provided are a plurality of recombinant bacteria. By improving the enzymatic activity of α-ketoglutarate dehydrogenase, succinic acid dehydrogenase and transaldolase therein and improving the ability of a cell to synthesize NADPH and ATP, the efficiency of the MEP pathway and the production capacity of terpenoid are improved. 1E. coli. A recombinant strain 1 , which is constructed by a method comprising: improving enzymatic activity of α-ketoglutarate dehydrogenase of or a mutant thereof , to obtain the recombinant strain 1.2. The recombinant strain 1 according to claim 1 , wherein:{'i': E. coli', 'E. coli, 'the enzymatic activity of α-ketoglutarate dehydrogenase of or a mutant thereof is improved by replacing an original regulatory part of an α-ketoglutarate dehydrogenase gene, sucAB, of or a mutant thereof with any one of the following artificial regulatory parts M1-46, M1-37 or M1-93; whereinartificial regulatory part M1-46 has a nucleotide sequence of SEQ ID NO: 14;artificial regulatory part M1-37 has a nucleotide sequence of SEQ ID NO: 10;artificial regulatory part M1-93 has a nucleotide sequence of SEQ ID NO: 11; andthe original regulatory part of α-ketoglutarate dehydrogenase gene is SEQ ID NO: 15.4. A recombinant strain 1-1-A or a recombinant strain 1-1-B claim 1 , wherein:{'claim-ref': [{'@idref': 'CLM-00003', ' ...

RECOMBINANT POLYNUCLEOTIDE SEQUENCE FOR PRODUCING ASTAXANTHIN AND USES THEREOF

Disclosed herein are recombinant polynucleotide sequences, vectors, host cells and methods for producing astaxanthin. The recombinant polynucleotide sequence is designed to provide a higher level of astaxanthin precursors via a shorter metabolic pathway, and thereby attains higher level of end products (e.g., astaxanthin) with desired stereoisomeric form and/or esterified form. 1. A method for producing astaxanthin , or a precursor or a derivative thereof , wherein the derivative of astaxanthin is an astaxanthin monoester or an astaxanthin diester; the method comprising , a first gene cassette comprising a first promoter, and a first nucleic acid sequence operatively linked to the first promoter and encoding a geranylgeranyl pyrophosphate synthase;', 'a second gene cassette comprising a second promoter, and a second nucleic acid sequence operatively linked to the second promoter and encoding a 3-hydroxy-3-methylglutaryl-coenzyme A reductase;', 'a third gene cassette comprising a third promoter, and a third nucleic acid sequence operatively linked to the third promoter and encoding a phytoene desaturase; and', 'a fourth gene cassette comprising a fourth promoter, and a fourth nucleic acid sequence operatively linked to the fourth promoter and encoding a bi-functional enzyme that possesses the respective functions of a phytoene synthase and a lycopene cyclase;', the 3′-end of each gene cassette of the recombinant polynucleotide sequence is homologous to the 5′-end of the next gene cassette downstream thereto;', 'the first, second, third, and fourth nucleic acid sequences respectively comprise the sequences of SEQ ID NOs: 1, 2, 3 and 4; and', {'i': 'Kluveromyces marxianus', 'the host cell is ; and'}], 'wherein,'}], '(1) introducing a recombinant polynucleotide sequence into a host cell, wherein the recombinant polynucleotide sequence comprises,'}(2) cultivating the host cell in a medium that comprises a material selected from the group consisting of glucose, galactose, ...

Increased Production of Terpenes and Terpenoids

This invention provides recombinant cells and methods for producing terpenes and terpenoids by increasing production or accumulation or both of isoprenoid precursors thereof. 149-. (canceled)50. A method for producing a terpene or a terpenoid in a recombinant host cell , comprising culturing the recombinant host cell under conditions wherein the terpene or terpenoid is produced in the recombinant host cell , wherein the recombinant host cell is genetically engineered to produce reduced expression of an endogenous farnesyl diphosphate (FPP) synthase , an endogenous geranyl diphosphate (GPP) synthase or an endogenous enzyme having both FPP synthase and GPP synthase activity.51. The method of claim 50 , wherein reduced expression is produced in the recombinant host cell by:(a) introducing into the recombinant host cell a heterologous genetic construct encoding the endogenous FPP synthase, the endogenous GPP synthase, or the endogenous enzyme having both FPP synthase and GPP synthase activity operably linked to an exogenous weak promoter, wherein the weak promoter is KEX2, PGK-1, GPD1, ADH1, ADH2, PYK1, TPi1, PDC1, TEF1, TEF2, FBA1, GAL1-10, CUP1, MET2, MET14, MET25, CYC1, GAL1-S, GAL1-L, TEF1, CAG, CMV, human UbiC, RSV, EF-1alpha, SV40, Mt1, Tet-On, Tet-Off, Mo-MLV-LTR, Mx1, progesterone, RU486 or Rapamycin-inducible promoter;(b) introducing into the recombinant host cell a heterologous genetic construct encoding the endogenous FPP synthase, the endogenous GPP synthase, or the endogenous enzyme having both FPP synthase and GPP synthase activity operably linked to a messenger RNA destabilizing motif, comprising a M1 motif of SEQ ID NO:28 and/or a M24 motif of SEQ ID NO:29; or {'br': None, 'sub': 1', '2', '3', '4', '5, '-X-X-X-X-X-;'}, '(c) introducing into the recombinant host cell a recombinant genetic construct, the construct comprising a gene encoding the endogenous FPP synthase, the endogenous GPP synthase, or the endogenous enzyme having both FPP synthase and GPP ...

METHODS FOR PRODUCING CAROTENOIDS FROM FERMENTATION BY-PRODUCTS

The present technology relates to methods for extracting carotenoids like β-carotene or lutein from oil obtained from/as a by-product derived from a feedstock material like starch-containing material in a processes for producing fermentation products by-products derived from a fermentative production process, in particular from an ethanol fermentation process, wherein the by-product is selected from the group consisting of distillers' wet grain (DWG), distillers' dried grains (DDG), distillers' solubles (DS), distillers' dried solubles (DDS), distillers' dried grain with solubles (DDGS), and mixtures thereof. 1. A method for extracting a carotenoid from oil obtained from a by-product derived from starch-containing material in a processes for producing fermentation products , wherein said method comprises the steps of:a) converting said starch containing material to fermentable sugars;b) fermenting said fermentable sugars with a microorganism;c) separating a by-product from fermentation;d) recovering oil from said by-product;e) contacting said oil with a solid adsorption material;f) separating said solid adsorption material containing said oil; andg) extracting said carotenoid from said adsorption material.2. (canceled)3. The method according to claim 1 , wherein the carotenoid to be extracted is a β-carotene or lutein.4. (canceled)5. The method according to claim 1 , wherein the by-product is selected from the group consisting of distillers' wet grain (DWG) claim 1 , distillers' dried grains (DDG) claim 1 , distillers' solubles (DS) claim 1 , distillers' dried solubles (DDS) claim 1 , distillers' dried grain with solubles (DDGS) claim 1 , and mixtures thereof.6. (canceled)7. The method according to claim 1 , wherein the fermentative production process is an ethanol production process.8. The method according to claim 1 , wherein the starch-containing material is selected from the group consisting of corn claim 1 , wheat claim 1 , barley claim 1 , triticale claim 1 , ...

PROCESS FOR PREPARING CAROTENOIDS BY SUBMERGED FERMENTATION WITH MIXED CULTURES OF (+) AND (-) STRAINS OF THE FUNGUS BLAKESLEA TRISPORA

The invention concerns a simple and effective process for preparing β-carotene or lycopene by submerged fermentation with mixed cultures of (+) and (−) strains of the fungus characterized by high productivity of the strains. The high productivity is achieved by virtue of the inventive regime, which acknowledges both the morphological state of the hemi-strains with their filamentous growth state when the strains are mixed (referred to as mating). 1Blakeslea trispora. A method for producing a carotenoid comprising one or more of β-carotene and lycopene through submerged fermentation with mixed cultures of (+) and (−) strains of the fungus and extracting the carotenoid from one or more of an obtained biomass and an oil phase of a fermentation sludge , the method comprising:{'i': 'Blakeslea trispora', 'separately pre-cultivating, the (−) and the (+) strains of a full morphological formation;'}inoculating and cultivating the (−) strain in a bioreactor;during the exponential growth of the (−) strain in the bioreactor, adding the (+) strain to the bioreactor in a volume ratio of 1:5 to 1:100 to induce carotenoid formation; andjointly fermenting the (−) and the (+) strains in the bioreactor a temperature between 18 to 24° C., the bioreactor being free from pH regulation and free from regulation of the oxygen partial pressure.2. The method according to claim 1 , wherein both the (−) and the (+) strains are fermented jointly in the bioreactor until carotenoid formation has been completed.3. The method according to or claim 1 , wherein the method steps of separately pre-cultivating the (−) and the (+) strains and inoculating and cultivating the (−) strain in the bioreactor are conducted at a temperature between 26-30° C.4. The method according to claim 1 , wherein the (+) strain is added to the (−) strain at a volume ratio of 1:5 to 1:20.5. The method according to claim 1 , wherein the (+) and (−) strains are jointly fermented for carotenoid formation at a temperature of 22° C ...

Polyphenol, terpenoid, glycoside, and alkaloid production by crocus sativus cell cultures

A method for producing metabolites of Crocus sativus ( C. sativus ) includes (i) selecting a cell line of C. sativus that produces one or more saffron metabolites in cell suspension culture, and (ii) growing the selected cell line in a suspension cell culture to produce the saffron metabolite.

Production of lutein in mixotrophic mode by Scenedesmus

Strains of microalgae belonging to the Scenedesmus genus grown in mixotrophic mode enable the production of lipids, in particular lutein. Methods for selecting and culturing the microalgae strains using variable and/or discontinuous supply of light, in particular in the form of flashes, are provided.

Method For Producing Carotenoids Each Having 50 Carbon Atoms

The present invention provides a method of producing a carotenoid having 50 carbon atoms which comprises culturing, in a medium, a cell transformed with a mutant phytoene desaturase gene and obtaining the carotenoid having 50 carbon atoms from the culture. The mutant phytoene desaturase gene has an introduced mutation to encode a mutant phytoene desaturase having an enhanced activity to desaturate a carotenoid backbone compound of 50 carbon atoms. 2. The method of producing a carotenoid having 50 carbon atoms claim 1 , according to claim 1 , wherein:the mutant phytoene desaturase gene has an introduced mutation to encode a mutant phytoene desaturase having an enhanced activity to desaturate a carotenoid backbone compound of 50 carbon atoms; andthe mutation causes a substitution of an amino acid corresponding to at least one amino acid selected from asparagine at position 304, phenylalanine at position 339, isoleucine at position 338, aspartic acid at position 395, and isoleucine at position 228 in an amino acid sequence set forth in SEQ ID NO: 1.3. The method of producing a carotenoid having 50 carbon atoms according to claim 1 , wherein:the mutant phytoene desaturase gene has an introduced mutation to encode a mutant phytoene desaturase having an enhanced activity to desaturate a carotenoid backbone compound of 50 carbon atoms; andthe mutation causes at least a substitution of an amino acid corresponding to asparagine at position 304 in SEQ ID NO: 1 by proline or serine.4. The method of producing a carotenoid having 50 carbon atoms according to claim 1 , wherein:the mutant phytoene desaturase gene has an introduced mutation to encode a mutant phytoene desaturase having an enhanced activity to desaturate a carotenoid backbone compound of 50 carbon atoms; and{'i': 'Pantoea ananatis.', 'the mutant phytoene desaturase gene is obtained by introducing the mutation into a phytoene desaturase gene derived from'}5Escherichia coli. The method of producing a carotenoid having ...

RECOMBINANT MICROORGANISM HAVING INCREASED ABILITY TO PRODUCE HYDROPHOBIC MATERIAL AND CELL-MEMBRANE ENGINEERING METHOD FOR PREPARATION THEREOF

Disclosed are a recombinant microorganism for producing a hydrophobic material, which is subjected to cell-membrane engineering in order to be imparted with at least one characteristic among an increase in a cell-membrane area, an increase in formation and secretion of an outer membrane vesicle, and an increase in formation of an inner membrane vesicle, and a cell-membrane engineering method for preparation thereof, whereby an insoluble hydrophobic material can be produced with high efficiency, the recombinant microorganism for high-efficiency production of carotenoids or violacein analogues is useful for producing natural pigments, antioxidants, antibiotics, cosmetic additives, anticancer agents, food additives, or nutritional supplements, and the natural pigment production technology developed herein achieves a great increase in production ability. Therefore, the present invention is effective at preparing a recombinant strain for efficient production of a variety of industrially and medically useful metabolites and at establishing an efficient preparation method.

METHODS FOR PRODUCING ZOOPLANKTON

A method of producing zooplankton biomass rich in a target compound, the method comprising the steps of: (a) providing one or more species of microalgae and/or cyanobacteria; (b) optionally stimulating the microalgae and/or cyanobacteria; (c) contacting the microalgae and/or cyanobacteria with one or more species of zooplankton which feed thereon; and (d) collecting a portion of the zooplankton; wherein waste from the zooplankton is fed back to the microalgae and/or cyanobacteria 1. A method of producing zooplankton biomass rich in a target compound , the method comprising the steps of:(a) providing one or more species of microalgae and/or cyanobacteria;(b) contacting the microalgae and/or cyanobacteria with one or more species of zooplankton which feed thereon; and(c) collecting a portion of the zooplankton,wherein waste from the zooplankton is fed back to the microalgae and/or cyanobacteria.2. A method according to wherein step (a) is carried out in a first chamber and step (b) is carried out in a second chamber.3. A method of providing a composition comprising a target compound claim 1 , the method comprising producing a zooplankton biomass rich in the target compound according to claim 1 , and extracting the target compound from the zooplankton.4. A system for producing biomass rich in a target compound claim 1 , the system comprising:(i) a first chamber comprising microalgae and/or cyanobacteria;(ii) a second chamber comprising zooplankton;(iii) means for carrying the microalgae and/or cyanobacteria from the first chamber to the second chamber;(iv) means for carrying waste from the second chamber to the first chamber; and(v) means for collecting a portion of the zooplankton.5. The system of claim 4 , wherein the system includes a closed loop.6. The method of claim 1 , wherein providing includes providing one or more species of microalgae.7. The method of claim 1 , wherein the target compound comprises lutein.8. The method of claim 1 , wherein the target ...

Extraction of Essential Oils

Essential oils are extracted from a biomass through milling in a solvent to form a solution of the essential oil in the solvent. The solvent is or is part of a cover than reduces oxidative and other degradation of the essential oil during milling and isolation. The solubilized essential oil may be allowed to adhere to the originating milled biomass to form a feed or nutritional supplement. The solvent may be evaporated from the solubilized essential oil to form an essential oil concentrate. This essential oil concentrate may be used directly, adhered to a different biomass than the originating biomass, or used in combination with pharmaceutical, nutritional, or feed preparations. The essential oil concentrate is preferably adhered to the different biomass through milling under a cover to reduce oxidative and other degradation. The essential oil may be astaxanthin, capsaicin compounds, or cannabinoids. 1. A method of extracting and isolating an essential oil from a biomass , the method comprising: the essential oil is soluble in the cover, and', 'the attrition mill includes milling media;, 'combining a biomass including an essential oil with a cover within an attrition mill, where'}milling the biomass and the cover in the attrition mill for a duration;reducing the particulate size of the biomass during the milling by repeatedly contacting the biomass with the milling media;releasing the essential oil from the biomass to the cover during the milling; 'the cover reduces the oxidation of the released essential oil in relation to the milling without the cover;', 'dissolving at least a portion of the essential oil released from the biomass in the cover during the milling, where'} a solution of the essential oil in a solvent, where the essential oil is a solute and the cover includes the solvent, and', 'a milled byproduct biomass; and, 'forming a mixture during the milling including'}separating the solution from the milled byproduct biomass, where the solution includes the ...

Method for producing carotenoid composition

The present invention provides an efficient method for industrially producing a naturally-derived carotenoid composition with a large amount of a carotenoid such as astaxanthin from a culture of a yeast of the genus Xanthophyllomyces without requiring any special extraction equipment and any complicated refinement process and without any need for organic solvents harmful to humans. Provided is a method for producing a carotenoid composition, including the steps of washing a carotenoid-containing yeast of the genus Xanthophyllomyces with an organic solvent (A) at 30° C. or lower, and extracting a carotenoid from the washed yeast with an organic solvent (B) at 10° C. to 70° C.

Method of Producing Natural B Carotene By Fermentation And Use Thereof

The present invention provides a method for producing and purifying β-carotene by fermentation and use thereof. The method comprises the following steps: a) separately inoculating the strains onto a PDA culture medium so as to obtain a spore suspension; b) propagating spores in a seeding tank so as to obtain seeds for fermentation; c) inoculating the seeds for fermentation onto a fermenter and fermenting said seeds; d) adjusting the fermentation liquid to be basic by using an organic or inorganic base, and filtering so as to obtain wet mycelia; e) treating the wet mycelia with a hydrophobic non-polar organic solvent; f) mixing the wet mycelia with an organic solvent of ester and obtaining a concentrated solution by extracting; g) adding a saturated monohydric alcohol into the concentrated solution, and filtering and crystallizing so as to obtain pure ss-carotene. The content of the ss-carotene in the present invention exceeds 96%, and the yield is above 85%. 1Blakeslea trispora. A method of producing and purifying β-carotene by fermentation , comprising the following steps:{'i': 'Blakeslea trispora', 'a) separately inoculating strains of “+” and “−” onto a PDA culture medium, culturing at 25-30° C. for 48-60 hours, and then eluting spores to a spore suspension by a sterile saline after growth of a great deal of spores;'}b) culturing and propagating spores of the fresh spore suspension in a seeding tank at pH=6.3˜6.8 and the cultivation temperature of 25-30° C. to seeds for fermentation, wherein the seed medium comprises corn starch, dextrose, vegetable oil, thiamine hydrochloride, magnesium sulfate and emulsifier;c) inoculating the seeds for fermentation in the seeding tank of step b) onto a fermenter and then fermenting so as to produce a fermentation liquid at pH=6.5˜6.8 and the cultivation temperature of 27-30° C.; wherein the medium is high-speed sheared and emulsified by a colloidal mill before inoculating, the medium of the fermenter comprises corn starch, ...

METHOD FOR PRODUCING ASTAXANTHIN

A method for increasing efficiency of a method for producing astaxanthin by culturing a microalga. A method for producing astaxanthin in which astaxanthin is produced in an algal body by culturing a microalga, wherein photoirradiation is performed using both a blue LED of peak wavelength from 420 to 500 nm and a red LED of peak wavelength from 620 to 690 nm, at least during an astaxanthin-producing culturing phase of a culturing period. The ratio of the blue LED of peak wavelength from 420 to 500 nm and the red LED of peak wavelength from 620 to 690 nm is preferably from 1:19 to 19:1 by photon flux density, and the photon flux densities are each preferably not less than 20 μmol/m/s. 1. A method for producing astaxanthin in which astaxanthin is produced in an algal body by culturing a microalga , wherein photoirradiation is performed using both a blue LED of peak wavelength from 420 to 500 nm and a red LED of peak wavelength from 620 to 690 nm , at least during an astaxanthin-producing culturing phase of a culturing period.2. The method for producing astaxanthin according to claim 1 , wherein a ratio of the blue LED of peak wavelength from 420 to 500 nm and the red LED of peak wavelength from 620 to 690 nm is from 1:19 to 19:1 by photon flux density.3. The method for producing astaxanthin according to claim 1 , wherein photon flux densities of the blue LED of peak wavelength from 420 to 500 nm and the red LED of peak wavelength from 620 to 690 nm are each not less than 20 μmol/m/s.4Haematococcus. The method for producing astaxanthin according to claim 1 , wherein the microalga is a genus alga.5. The method for producing astaxanthin according to claim 1 , wherein an astaxanthin production quantity per volume of culture solution is not less than 100 mg/L.6. The method for producing astaxanthin according to claim 5 , wherein the astaxanthin production quantity per volume of culture solution is not less than 300 mg/L.7. A culture solution of a microalga claim 5 , wherein ...

METHODS FOR PRODUCING BIOMASS RICH IN DHA, PALMITIC ACID AND PROTEIN USING A EUKARYOTIC MICROORGANISM

Provided herein are eukaryotic microorganisms having a simple lipid profile comprising long chain fatty acids (LCFAs). Also provided are compositions and cultures comprising the eukaryotic microorganisms as well as methods of using the eukaryotic microorganisms. 127.-. (canceled)28. A method of making a lipid composition , the method comprising(a) culturing a eukaryotic microorganism in a heterotrophic medium to produce a biomass comprising the lipid composition comprising at least 35% DHA and a simple lipid profile comprising long chain fatty acids (LCFAs), wherein the simple lipid profile comprises triglycerides and wherein greater than 95% of the triglycerides are comprised of myristic acid (C14:0), palmitic acid (C16:0), docosapentaenoic acid n-6 (C22:5n-6, DPAn6), and docosahexaenoic acid (C22:6n-3, DHA),(b) and isolating the lipid composition.29. The method of claim 28 , wherein the heterotrophic medium contains less than 3.75 g/L chloride.30. The method of claim 28 , wherein the biomass productivity of the cultured micororganisms is greater than 0.65 g/L/h.31. The method of claim 28 , wherein the triglyceride productivity of the cultured microorganisms is greater than 0.3 g/L/h.3236.-. (canceled)37. The method of claim 28 , wherein the simple lipid profile comprises less than 3% of each of lauric acid (C12:0) claim 28 , pentadecylic acid (C15:0) claim 28 , palmitoleic acid (C16:1) claim 28 , margaric acid (C17:0) claim 28 , stearic acid (C18:0) claim 28 , vaccenic acid (C18:1n-7) claim 28 , oleic acid (C18:1n-9) claim 28 , γ-linolenic acid (C18:3n-6) claim 28 , α-linolenic acid (C18:3n-3) claim 28 , stearidonic acid (C18:4) claim 28 , arachidic acid (C20:0) claim 28 , dihomo-γ-linolenic acid (C20:3n-6) claim 28 , arachidonic acid (C20:4n-6 claim 28 , ARA) claim 28 , eicosapentaenoic acid (C20:5n-3 claim 28 , EPA) claim 28 , behenic acid (C22:0) claim 28 , docosatetraenoic acid (C22:4) claim 28 , docosapentaenoic acid n3 (C22:5n-3 claim 28 , DPAn3) claim 28 , ...

METHOD FOR PRODUCING FERMENTED CAROTENOID USING CAROTENOID-PRODUCING BACTERIA OBTAINED BY USING COBALT-CONTAINING CULTURING MEDIUM

The present invention provides a method for high yield and stable production of carotenoids through microbial culture. The present invention provides a method for culture of carotenoid-producing bacteria, which comprises culturing carotenoid-producing bacteria in a medium containing cobalt or a cobalt salt at a concentration of 0.005 μmol/L to 20 μmol/L. 111-. (canceled)12Paracoccus. A method for stable preparation of microbial cells containing carotenoids in an amount of at least 36 mg/g in dry microbial cells , comprising culturing bacteria belonging to the genus in a medium containing cobalt or a cobalt salt at a concentration of 0.005 μmol/L to 21 μmol/L.13. The method of claim 12 , wherein the cobalt or cobalt salt has been added at a concentration of 0.005 μmol/L to 21 μmol/L in the medium.14. The method according to claim 12 , wherein the concentration of cobalt or a cobalt salt in the medium is 0.005 μmol/L to 8 μmol/L.15. The method according to claim 12 , wherein the carotenoids comprise at least one selected from the group consisting of astaxanthin claim 12 , canthaxanthin claim 12 , zeaxanthin claim 12 , β-cryptoxanthin claim 12 , lycopene claim 12 , β-carotene claim 12 , adonirubin claim 12 , adonixanthin claim 12 , echinenone claim 12 , asteroidenone and 3-hydroxyechinenone.16Paracoccus. The method according to claim 12 , wherein the bacteria belonging to the genus are those comprising DNA which corresponds to 16S ribosomal RNA and whose nucleotide sequence shares a homology of 95% or higher with the nucleotide sequence shown in SEQ ID NO: 1.17Paracoccus. The method according to claim 12 , wherein the bacteria belonging to the genus are those of the strain E-396 (FERM BP-4283) or the strain A-581-1 (FERM BP-4671) or a mutant strain thereof.18Paracoccus. A method for production of carotenoids claim 12 , comprising culturing bacteria belonging to the genus in a medium containing cobalt or a cobalt salt at a concentration of 0.005 μmol/L to 21 μmol/L to ...

METHOD OF PRODUCING TERPENES OR TERPENOIDS

The present invention relates to a recombinant bacterium exhibiting enhanced 2-C-methyl-D-erythritol 4-phosphate/1-deoxy-D-xylulose 5-phosphate (MEP/DXP) pathway, and its use for producing terpene or terpenoid compounds. 122-. (canceled)23Deinococcus. A method of producing a terpene or terpenoid comprising (i) culturing a recombinant bacterium that is genetically modified to increase 1-deoxyxylulose 5-phosphate synthase (DXS) activity and/or isopentenyl pyrophosphatase isomerase (IPP isomerase) activity , under conditions suitable to produce the terpene or terpenoid and optionally (ii) recovering the terpene or terpenoid.24Deinococcus. The method of claim 23 , wherein the recombinant bacterium overexpresses a native claim 23 , homologous or heterologous idi gene.25Deinococcus. The method of claim 23 , wherein the recombinant bacterium overexpresses a native claim 23 , homologous or heterologous dxs gene.26Deinococcus. The method of claim 23 , wherein the recombinant bacterium expresses an improved DXS enzyme.27. The method of claim 26 , wherein the improved DXS enzyme is a mutant DXS enzyme comprising a cysteine at position corresponding to position 244 of SEQ ID NO: 52.28DeinococcusD. radiopugnansD. yunweiensisD. geothermalis. The method of claim 26 , wherein the recombinant bacterium expresses a gene encoding the R244C mutant of the DXP synthase from (SEQ ID NO: 8) claim 26 , a gene encoding the R238C mutant of the DXP synthase from (SEQ ID NO: 14) or a gene encoding the R241C mutant of the DXP synthase from (SEQ ID NO: 56).29Deinococcus. The method of claim 23 , wherein the recombinant bacterium expresses an improved DXS enzyme and overexpresses a native claim 23 , homologous or heterologous idi gene.30Deinococcus. The method of claim 23 , wherein the recombinant bacterium overexpresses a native claim 23 , homologous or heterologous gene encoding FPP synthase.31. The method of claim 30 , wherein the gene encoding FPP synthase encodes a polypeptide selected from ...

TRANSFORMED SYNECHOCOCCUS ELONGATUS STRAINS HAVING IMPROVED PRODUCTIVITY OF FARNESENE AND USE THEREOF

The present disclosure relates to the transformed strain of capable of mass production of farnesene. The transformed strain of the present disclosure is characterized by having the ability to mass produce farnesene using carbon dioxide as an independent carbon source. In particular, the strain is economically effective because it uses carbon dioxide present in light and air as a carbon source. There is an eco-friendly effect since it can be used for eliminating or reducing carbon dioxide in the atmosphere using microorganisms. Further, the strain of the present disclosure has a rapid growth rate and excellent ability to fix carbon dioxide compared with other microorganisms, thereby being utilized in various fields such as food, medicine, pharmacy, biofuel, and chemistry. 1Synechococcus elongatus. A strain comprising a farnesene synthase gene (FS) consisting of the nucleotide sequence represented by SEQ ID NO: 4.2Synechococcus elongatus. The strain according to claim 1 , the strain further comprising a deoxyxylulose-5-phosphate synthase gene (dxs) consisting of the nucleotide sequence represented by SEQ ID NO: 1.3Synechococcus elongatus. The strain according to claim 2 , the strain further comprising an isopentenyl-diphosphate delta isomerase (idi) gene consisting of the nucleotide sequence represented by SEQ ID NO: 2 and a farnesyl diphosphate synthase (ispA) gene consisting of the nucleotide sequence represented by SEQ ID NO: 3.4Synechococcus elongatus. The strain according to claim 3 , wherein the strain is a strain of Accession No. KCCM 12133P.5Synechococcus elongatus. The strain according to claim 1 , wherein the strain is transformed with a pSe2Bb1k-AFS recombinant vector having the vector map of .6Synechococcus elongatus. The strain according to claim 2 , wherein the strain is further transformed with a pSe1Bb1s-dxs recombinant vector having the vector map of .7Synechococcus elongatus. The strain according to claim 3 , wherein the strain is further transformed ...

FILAMENTOUS FUNGAL MUTANTS WITH IMPROVED HOMOLOGOUS RECOMBINATION EFFICIENCY

The present invention relates to a method for increasing the efficiency of targeted integration of a polynucleotide to a pre-determined site into the genome of a filamentous fungal cell with a preference for NHR, wherein said polynucleotide has a region of homology with said pre-determined site, comprising steering an integration pathway towards HR. The present invention also relates to a mutant filamentous fungus originating from a parent cell, said mutant having an HR pathway with elevated efficiency and/or an NHR pathway with a lowered efficiency and/or a NHR/HR ratio with decreased efficiency as compared to said HR and/or NHR efficiency and/or NHR/HR ratio of said parent cell under the same conditions. 1Penicillium. Method for increasing the efficiency of targeted integration of a polynucleotide to a pre-determined site into the genome of a filamentous fungal cell with a preference for NHR , wherein said polynucleotide has a region of homology with said pre-determined site , wherein said filamentous fungal cell belongs the genus , comprising providing a mutant of a parent filamentous fungal cell , wherein the ratio of NHR/HR is decreased in the mutant as compared to said ratio in said parent organism measured under the same conditions , wherein said mutant is deficient in a gene encoding a component involved in NHR , and/or has a decreased level of a component involved in NHR.2. The method of claim 1 , wherein the steering comprises providing a mutant of a parent filamentous fungal cell claim 1 , wherein the ratio of NHR/HR is decreased in the mutant as compared to said ratio in said parent organism measured under the same conditions.3. The method of claim 1 , wherein the steering comprises providing a mutant which is deficient in a gene encoding a component involved in NHR claim 1 , and/or has a decreased level of a component involved in NHR.4. The method of claim 1 , wherein the mutant is claim 1 , preferably inducibly claim 1 , deficient in at least one of ...

IMPROVED PRODUCTION PROCESSES OF HAEMATOCOCCUS CELL COMPONENTS

The technology disclosed herein relates to novel methods and compositions for the production processes of algae cell components from algae of the genus . The extractability of algae cellular components is improved and the process is optimized and accelerated by using the enzyme compositions according to the present invention. 1. A method for improving the extractability of an algae cellular component from an algae biomass comprising the steps of:a) Subjecting the algae biomass to an enzyme composition comprising a mannanase and/or a lipase and/or a laminarinase, {'i': 'Haematococcus.', 'wherein the algae belongs to the genus'}, 'b) Extracting the cellular composition from the algae biomass,'}2. The method according to claim 1 , whereby the cellular component is selected from the group consisting of pigments claim 1 , carotenoids claim 1 , starch claim 1 , lipids claim 1 , poly unsaturated fatty acids (PUFA) claim 1 , proteins claim 1 , vitamins and mineral nutrients.3. The method according to claim 1 , whereby the cellular composition is a carotenoid.4. The method according to claim 3 , whereby the carotenoid is astaxanthin.5. The method according to claim 4 , whereby the carotenoid is astaxanthin in form of a (3S claim 4 ,3′S) isomer.6. The method according to claim 1 , whereby the enzyme composition further comprises a protease.7. The method according to claim 1 , whereby the enzyme composition further comprises a cellulase.8. The method according to claim 1 , whereby the enzyme composition further comprises a pectinase.9. The method according to claim 1 , whereby the enzyme composition comprises the mannanase and a protease.10. The method according to claim 1 , whereby the enzyme composition comprises the mannanase claim 1 , a pectinase and a cellulase.11. The method according to claim 1 , whereby the enzyme composition comprises the mannanase claim 1 , the laminarinase and the lipase.12Haematococcus pluvialis.. The method according to claim 1 , whereby the algae ...

Microorganism comprising gene for coding enzyme involved in producing retinoid and method for producing retinoid by using same

The present invention relates to a microorganism comprising a gene for coding an enzyme involved in producing retinoid and a method for producing retinoid by using the same, and more specifically, to: a microorganism capable of mass-producing retinoid at a remarkable efficiency by comprising a gene for coding an enzyme involved in producing retinoid; and a method for producing retinoid by using the same.

PRODUCTION OF DOCOSAHEXAENOIC ACID AND/OR EICOSAPENTAENOIC ACID AND/OR CAROTENOIDS IN MIXOTROPHIC MODE BY NITZSCHIA

New strains of microalgae belonging to the genus allow high-yield production of lipids such as docosahexaenoic acid (DHA) and eicosapentaenoic acid (EPA), and/or carotenoids such as fucoxanthin. Biomass containing the microalgae can be charged with fucoxanthin representing more than 0.2% by weight of the dry matter. 1NitzschiaNitzschia. Biomass consisting essentially of microalgae of the genus and water , wherein said microalgae of the genus are charged with fucoxanthin , said fucoxanthin representing more than 0.2% by weight of the biomass dry matter.2. The biomass of claim 1 , wherein said fucoxanthin represents more than 0.25% by weight of the biomass dry matter.3. The biomass of claim 1 , wherein the biomass comprises at least 30 g/L of dry matter.4. The biomass of claim 1 , wherein the biomass is product of fermentation.5. The biomass of claim 4 , wherein the biomass is obtained from a culture that has been cultured in mixotrophic mode over several generations in a culture medium comprising a carbon-containing substrate.6Nitzschia. The biomass of claim 1 , wherein said microalgae of the genus is the strain FCC 810 claim 1 , deposited with the CCAP (Culture Collection of Algae and Protozoa) under the accession number CCAP 1052/21. The invention relates to a method of culture in mixotrophic mode, in particular in the presence of discontinuous and/or variable illumination with light, of a microalga of the genus in particular of the species The method makes it possible to obtain a high yield of biomass and enrichment of the thus cultured microalgae in lipids and more particularly in docosahexanoic acid (DHA) and/or eicosapentaenoic acid (EPA). The method also makes it possible to obtain an enrichment in carotenoids of the thus cultured microalgae, and more particularly, in fucoxanthin. The method thus makes it possible to select strains of in particular of with mixotrophic character, and having a high yield of lipids and more particularly of polyunsaturated fatty ...

METHOD FOR PRODUCING COENZYME Q10

A method of producing coenzyme Q10 includes contacting an extract from a coenzyme Q10-producing microorganism with an adsorbent (A) such that the adsorbent (A) adsorbs a component of the extract other than coenzyme Q10, and that coenzyme Q10 is obtained. The adsorbent (A) includes aluminum silicate at a content of 50% or more. 1. A method of producing coenzyme Q10 , comprising:contacting an extract from a coenzyme Q10-producing microorganism with an adsorbent (A) such that the adsorbent (A) adsorbs a component of the extract other than coenzyme Q10, and that coenzyme Q10 is obtained,wherein the adsorbent (A) includes aluminum silicate at a content of 50% or more.2. The method of claim 1 , further comprising:contacting the extract with an adsorbent (B).3. The method of claim 1 , wherein the contacting comprises contacting the extract with a mixture including the adsorbent (A) and an adsorbent (B).4. The method of claim 2 , wherein the adsorbent (B) comprises at least one selected from the group consisting of activated carbon claim 2 , an adsorbent including activated carbon as a main component claim 2 , a synthetic adsorbent obtained by copolymerizing styrene and divinylbenzene claim 2 , a phenol-formaldehyde resin adsorbent claim 2 , an aldehyde adsorbent claim 2 , magnesium silicate claim 2 , aluminum hydroxide claim 2 , aluminum oxide claim 2 , magnesium oxide claim 2 , diatomite claim 2 , activated alumina claim 2 , silica gel claim 2 , silica-magnesia gel claim 2 , an adsorbent obtained by chemically binding an alkyl group or an allyl group on an inorganic carrier claim 2 , an aromatic adsorbent claim 2 , a methacrylate ester adsorbent claim 2 , synthetic zeolite claim 2 , and a pearlite filter medium.5. The method of claim 3 , wherein the adsorbent (B) comprises at least one selected from the group consisting of activated carbon claim 3 , an adsorbent including activated carbon as a main component claim 3 , a synthetic adsorbent obtained by copolymerizing ...

ACETYL TRANSFERASES AND THEIR USE FOR PRODUCING CAROTENOIDS

The present invention relates to acetyl transferases, nucleic acid sequences coding therefore, expression constructs and vectors comprising these sequences, microorganisms transformed therewith and use thereof. 115-. (canceled)16. A process for conversion of at least one carotenoid containing (a) at least one β-ionone and/or at least one ε-ionone ring and (b) at least one ring-associated hydroxyl group into the corresponding mono- or di-acetylated carotenoid molecule , comprising transforming an oleaginous carotenoid-producing fungus or bacterium with a nucleic acid molecule expressing an enzyme having acetyl transferase activity with at least 80% sequence identity to SEQ ID NO: 2 or 4.17. The process according to claim 16 , wherein the at least one carotenoid is selected from the group consisting of zeaxanthin claim 16 , astaxanthin claim 16 , lutein claim 16 , and beta-cryptoxanthin.18SaccharomycesSaccharomyces cerevisiaeSaccharomyces bayanusSaccharomyces mikataeSaccharomyces kudriavzevii. The process according to claim 16 , wherein the enzyme having acetyl transferase activity is selected from a acetyl transferase (ATF) enzyme selected from ATF claim 16 , ATF claim 16 , ATF or ATF.19Yarrowia.. The process according to claim 16 , wherein the oleaginous carotenoid-producing fungus is selected from20Paracoccus.. The process according to claim 16 , wherein the oleaginous carotenoid-producing bacterium is selected from21. The process according to claim 17 , wherein the carotenoid is astaxanthin and at least about 9% is in the form of mono-acetylated astaxanthin based on total astaxanthin.22. The process according to claim 17 , wherein the carotenoid is astaxanthin and at least about 6% is in the form of di-acetylated astaxanthin based on total astaxanthin.23. The process according to claim 17 , wherein the carotenoid is zeaxanthin and at least about 4% is in the form of mono-acetylated zeaxanthin based on total zeaxanthin.24. The process according to claim 17 , ...

METHOD FOR HARVESTING ORGANIC COMPOUNDS FROM GENETICALLY MODIFIED ORGANISMS

Disclosed herein are embodiments for a novel method of producing an organic compound, including harvesting at least one organic compound from an organism or cell line genetically engineered with a gene for at least one proton-pump protein. 1. A method of producing an organic compound comprising ,harvesting at least one organic compound from an organism or cell line genetically engineered with a gene for at least one proton-pump protein.2. The method of claim 1 , wherein the at least one proton-pump protein is a light-driven proton-pump protein.3. The method of claim 1 , wherein the at least one proton-pump protein is an archaerhodopsin claim 1 , bacteriorhodopsin claim 1 , opsin claim 1 , proteorhodopsin claim 1 , rhodopsin claim 1 , xanthorhodopsin claim 1 , homologs thereof or combinations thereof.4. The method of claim 3 , wherein the at least one proton-pump protein absorbs light between about 100 nm and about 1 μm.5. The method of claim 4 , wherein the at least one proton-pump protein absorbs light between about 300 nm and about 750 nm.6. The method of claim 5 , wherein the at least one proton-pump protein absorbs light between about 450 nm and about 650 nm.7. The method of claim 6 , wherein the at least one proton-pump protein absorbs light between about 450 nm and about 550 nm.8. The method of claim 6 , wherein the at least one proton-pump protein absorbs light between about 550 nm and about 600 nm.9. The method of claim 1 , wherein the at least one organic compound is a biologic or a biofuel10. The method of claim 9 , wherein the biologic is selected from the group consisting of allergenics claim 9 , antibodies claim 9 , blood products or derivative thereof claim 9 , enzymes claim 9 , growth factors claim 9 , hormones claim 9 , immunomodulators claim 9 , interferons claim 9 , interleukins claim 9 , polypeptides claim 9 , proteins claim 9 , serum claim 9 , tissues claim 9 , toxins and vaccines.11. The method of claim 10 , wherein the antibody is selected from ...

UNCOUPLED CELL CULTURE METHOD

Disclosed is a method for producing biomass by culturing cells in mixotrophic conditions, in particular in the presence of discontinuous and/or variable lighting conditions, and/or in heterotrophic conditions, making it possible to obtain an increase in both the cellular concentration and the production of molecules of interest. 123-. (canceled)24. Method for the production of biomass , comprising:a) the culture of the cells in continuous mode under mixotrophic or heterotrophic conditions in a fermenter, thenb) the continuous and successively feeding of n fermenters operating in semi-continuous mode, n being an integer equal to or greater than 2, with the cells produced in step a) and their culture under mixotrophic conditionswherein the culture of the cells under mixotrophic conditions is carried out under conditions of illumination that is discontinuous and/or variable over time.25. Method according to claim 24 , wherein the illumination has variations in intensity the amplitude of which is comprised between 5 and 1 claim 24 ,000 μmol·m-2·s-1 claim 24 , these variations taking place between 2 and 3 claim 24 ,600.26. Method according to claim 24 , wherein the illumination is in the form of flashes.27. Method according to claim 24 , wherein the culture in step a) is carried out in the presence of flashes having an intensity of 50 to 200 μmol·m-2·s-1 and a duration of approximately 1/10th of a second to five minutes claim 24 , and between 2 and 3 claim 24 ,600 flashes per hour.28. Method according to claim 24 , wherein the culture in step b) is carried out in the presence of flashes having an intensity of 50 to 2 claim 24 ,000 μmol·m-2·s-1 and a duration of approximately 1/10th of a second to five minutes claim 24 , and between 2 and 3 claim 24 ,600 flashes per hour.29. Method according to claim 24 , wherein the culture in steps a) and/or b) is carried out in the presence of an organic carbon-containing substrate at a concentration from 5 mM to 1.1 M.30. Method ...

Relatively inexpensive process to turn green cells of Haematococcus pluvialis into astaxanthin accumulating red cysts

This invention discloses an innovative budgetary process on how to turn green cells of into red cysts that contain the astaxanthin of the highest rate. With this finding, green motile cells are converted into dormant red cysts via addition of resting cysts into the culture with green cells. Transformation of green cells of into red dormant cells with this invented method can be achieved without need of high-intensity light. Light intensity can be reduced for up 15 times to approach the similar results within the same time-period. The least concentration of 1% of red cysts to green culture supports the economically most approved run. Shaking of the cultures of green cells with added red cysts stimulates the transformation process. Inoculation of higher concentration of red cysts into green culture of shorten the period of greens conversion into a biomass, enriched with astaxanthin. 1Shaking of the green cultures after addition of red cells stimulates transmission of motile di-flagellates into resting stage.{'sup': '−2', 'Combination of high-intensity light (300-600 lum ft) with inoculation of dormant cells in green culture reduces time-frame up to 2 times, and if to synchronize these together with shaking, it reduces period of greens transformation into dormant stage in up to 3 times.'}. Addition of red dormant cysts to green culture of Haematococcus pluvialis demonstrates stimulating effect to turn green di-flagellates into resting cells even under low light conditions (from 600 lum ftto 40 lum ft) with the least ratio of 1:100 (dormant cysts : green cells of cells density 70.0*10L) that requires similar period for green motile cells to convert into dormant cysts under high light stress. Nature has created a number of molecular mechanisms to accumulate specific secondary metabolites (i.e., pigments, vitamins, or lipids) to resist unfavorable environmental fluctuations (e.g. high light, salinity, nutrient stress, and high carbon/nitrogen ratio). One of such a ...

Novel strain of aurantiochytrium

Disclosed are novel strains of protists belonging to the Aurantiochytrium genus that allow the high yield production of lipids and carotenoids, in particular docosahexaenoic acid (DHA) and canthaxanthin and/or astaxanthin, in mixotrophic mode, in particular, using variable and/or discontinuous provision of light, in particular in the form of flashes.

Astaxanthin Over-Producing Strains of Phaffia Rhodozyma

The present invention relates to novel yeast strains of which produce high amounts of carotenoids, in particular high amounts astaxanthin. These novel strains are capable of producing increasing amounts of carotenoids in the presence of increasing concentrations of carbon source. 1Phaffia rhodozyma. A yeast strain of , wherein the yeast strain is characterized in that it is capable of producing a greater amount of carotenoid when cultivated in a yeast growth medium comprising at least 3% (w/v) carbon source compared to when cultivated in a yeast growth medium comprising 1% (w/v) carbon source; wherein the yeast strain comprises a genome comprising:i) a point mutation at position 2028187 in scaffold 69, at the upstream region of gene XDEN_04715, and/orii) a point mutation at position 1540450 in scaffold 79 at gene XDEN_05955.2. The yeast strain of claim 1 , wherein the yeast strain further comprises a genome comprising at least one claim 1 , at least two claim 1 , at least three claim 1 , or at least four of iii) to vi) defined in the following:iii) two-point mutations at positions 318703 and 318738 in scaffold 242, upstream to gene XDEN_00738;iv) two-point mutations at positions 855 and 1020 in scaffold 88, downstream to gene XDEN_06229;v) a deletion of two bases immediately following position 213879 in scaffold 162, located at the gene XDEN_00195; andvi) two-point mutations at positions 84078 and 84206 in scaffold 24, at the gene XDEN_01573.3. The yeast strain of claim 1 , wherein the point mutation at position 1540450 in scaffold 79 at gene XDEN_05955 is a G to A change claim 1 , resulting in an amino acid exchange of proline to serine.4. The yeast strain of claim 1 , wherein the point mutation(s) is (are) present in at least one claim 1 , at least two claim 1 , or in all alleles of said gene(s).5. The yeast strain of claim 1 , comprising a genome comprising at least one claim 1 , at least two claim 1 , at least three claim 1 , at least four claim 1 , at least ...

METHODS FOR IMPROVING TITER AND PURITY OF BETA CAROTENE FERMENTATION IN BLAKESLEA TRISPORA (BETA-CAROTENE FERMENTATION METHOD)

The present invention is related to the production of bio-based carotenoids, particularly to methods for enhancing the titer and purity of beta-carotene fermentation in a suitable host cell. 1Blakeslea trispora. A process for fermentative production of carotenoids in a carotenoid-producing host cell , preferably a native carotenoid producer , more preferably , wherein the fermentation is performed in the presence of an effective amount of curcumin being added during the fermentation , preferably curcumin concentrations below about 1.0% (w/v) is added.2. A process according to claim 1 , wherein the curcumin concentration added during fermentation is in the range of about 0.01 to 1.0% (w/v) claim 1 , preferably in the range of about 0.3 to 1.0% (w/v).3. A process according to claim 1 , wherein the production of beta-carotene is increased by at least about 12% compared to fermentation without addition of curcumin.4. A process according to claim 1 , wherein the formation of 7 claim 1 ,8-dihydro-beta-carotene during the fermentation is reduced.5. The process according to claim 4 , wherein the percentage of 7 claim 4 ,8-dihydro-beta-carotene based on total carotenoids formed during the fermentation is reduced to about 7% or less claim 4 , preferably to about 3% based on total carotenoids.6. The process according to claim 4 , wherein the percentage of 7 claim 4 ,8-dihydro-beta-carotene formed during the fermentation is reduced by about 70% compared to fermentation without addition of curcumin.7. A process according to claim 1 , wherein the percentage of beta-carotene based on total carotenoids formed during the fermentation is increased claim 1 , preferably increased by about 3 to 11%.8. The process according to claim 7 , wherein the percentage of beta-carotene based on total carotenoids formed during fermentation is in the range of about 90% or more compared to fermentation without addition of curcumin.9. The process according to claim 1 , wherein the fermentation is ...

RECOMBINANT POLYNUCLEOTIDE SEQUENCE FOR PRODUCING ASTAXANTHIN AND USES THEREOF

Disclosed herein are recombinant polynucleotide sequences, vectors, host cells and methods for producing astaxanthin. The recombinant polynucleotide sequence is designed to provide a higher level of astaxanthin precursors via a shorter metabolic pathway, and thereby attains higher level of end products (e.g., astaxanthin) with desired stereoisomeric form and/or esterified form. 1. A recombinant polynucleotide sequence for producing astaxanthin , a precursor or a derivative thereof in a host cell , comprising ,a first gene cassette comprising a first promoter, and a first nucleic acid sequence operatively linked to the first promoter and encodes a geranylgeranyl pyrophosphate synthase;a second gene cassette comprising a second promoter, and a second nucleic acid sequence operatively linked to the second promoter and encodes a 3-hydroxy-3-methylglutaryl-coenzyme A reductase;a third gene cassette comprising a third promoter, and a third nucleic acid sequence operatively linked to the third promoter and encodes a phytoene desaturase; anda fourth gene cassette comprising a fourth promoter, and a fourth nucleic acid sequence operatively linked to the fourth promoter and encodes a bi-functional enzyme that possesses the respective functions of a phytoene synthase and a lycopene cyclase; andthe 3′-end of each gene cassette of the recombinant polynucleotide sequence is homologous to the 5′-end of the next gene cassette downstream thereto,wherein the first, second, third, and fourth nucleic acid sequences respectively comprise the sequences of SEQ ID NOs: 1, 2, 3, and 4.2. The recombinant polynucleotide sequence of claim 1 , whereinthe astaxanthin is 3′S, 3′S-astaxanthin or 3′R3′R, 3′R-astaxanthin;the precursor of astaxanthin is geranylgeranyl-pyrophosphate, phenicoxanthin, lycopene, echinenone, canthaxanthin, phytoene, zeaxanthin, β-cryptoxanthin, or β-carotene; andthe derivative of astaxanthin is an astaxanthin monoester or an astaxanthin diester.3. The recombinant ...

MUTANT HAVING ABILITY TO OVERPRODUCE CAROTENOIDS AND METHOD FOR PRODUCING CAROTENOIDS BY USING SAME

Provided is a mutant having an ability to overproduce carotenoids and a method for producing carotenoids by using the mutant. The mutant, of which mutations are induced by irradiation after being transformed with a recombinant vector according to the subject matter, has an excellent ability to produce carotenoids and can be mass-produced, and thus can be useful in various industrial fields, which use carotenoids, such as cosmetics, food, and feed. 1. A mutant strain having an ability to over-produce a carotenoid , which is mutagenized by irradiation ,wherein the mutant strain includes a CrtE gene encoding geranylgeranyl pyrophosphate synthase; a CrtB gene encoding phytoene synthase; a CrtI gene encoding phytoene desaturase; a CrtY gene encoding lycopene beta cyclase; a CrtX gene encoding glucosyl transferase; and a CrtZ gene encoding beta-carotene hydroxylase, and is transformed with a recombinant vector for production of a carotenoid.2Escherichia coli. The mutant strain of claim 1 , wherein the mutant strain is whose accession number is KCTC 12683BP.3Pantoea agglomerans.. The mutant strain of claim 1 , wherein the CrtE gene claim 1 , the CrtB gene claim 1 , the CrtI gene claim 1 , the CrtY gene claim 1 , the CrtX gene claim 1 , and the CrtZ gene are derived from4. The mutant strain of claim 3 , wherein the CrtE gene consists of a nucleotide sequence as set forth in SEQ ID NO: 1.5. The mutant strain of claim 3 , wherein the CrtB gene consists of a nucleotide sequence as set forth in SEQ ID NO: 2.6. The mutant strain of claim 3 , wherein the CrtI gene consists of a nucleotide sequence as set forth in SEQ ID NO: 3.7. The mutant strain of claim 3 , wherein the CrtY gene consists of a nucleotide sequence as set forth in SEQ ID NO: 4.8. The mutant strain of claim 3 , wherein the CrtX gene consists of a nucleotide sequence as set forth in SEQ ID NO: 5.9. The mutant strain of claim 3 , wherein the CrtZ gene consists of a nucleotide sequence as set forth in SEQ ID NO: 6.10. ...

PRODUCTION OF CAROTENOIDS IN OLEAGINOUS YEAST AND FUNGI

The present invention provides systems for producing engineered oleaginous yeast or fungi that express retinolic compounds. 1Saccharomyces cerevisiae. An engineered strain that produces at least one retinolic compound selected from the group consisting of retinol , retinal , retinoic acid , retinyl palmitate and combinations thereof , the strain comprising at least one retinologenic modification selected from the group consisting of increased expression or activity of a beta-carotene 15 ,15′-monooxygenase polypeptide , increased expression or activity of a retinol dehydrogenase polypeptide , and combinations thereof;wherein the engineered strain can accumulate lipid to at least about 20% of its dry cell weight; andwherein as a result of genetic engineering, the engineered strain produces the at least one retinolic compound to a level at least about 1% of its dry cell weight.2Saccharomyces cerevisiae. The engineered strain of claim 1 , wherein the strain does not naturally produce the at least one retinolic compound.3Saccharomyces cerevisiae. The engineered strain of claim 1 , further comprising at least one oleaginic modification.4Saccharomyces cerevisiae. The engineered strain of claim 3 , wherein the at least one oleaginic modification increases or decreases expression or activity of at least one oleaginic polypeptide claim 3 , selected from the group consisting of acetyl-CoA carboxylase polypeptide claim 3 , pyruvate decarboxylase polypeptide claim 3 , isocitrate dehydrogenase polypeptide claim 3 , ATP-citrate lyase polypeptide claim 3 , malic enzyme polypeptide claim 3 , AMP deaminase polypeptide claim 3 , malate dehydrogenase polypeptide claim 3 , glucose-6-phosphate dehydrogenase polypeptide claim 3 , 6-phosphogluconate dehydrogenase polypeptide claim 3 , fructose 1 claim 3 ,6 bisphosphatase polypeptide claim 3 , NADH kinase polypeptide claim 3 , transhydrogenase polypeptide claim 3 , acyl-CoA:diacylglycerol acyltransferase polypeptide claim 3 , phospholipid: ...

DRIED BACTERIAL CELL POWDER CONTAINING A CAROTENOID AND METHOD FOR PRODUCING THE SAME

According to the present invention, a powder containing a carotenoid for feed having improved color enhancing ability, and a method for producing the same are provided. A method for producing a dried bacterial cell powder containing a carotenoid, comprising a step of drying via conductive heat transfer and a pulverization step and a dried bacterial cell powder produced by the method are provided. 1. A method for producing a dried bacterial cell powder containing a carotenoid , said method comprising the step of bringing bacterial cells of a carotenoid-producing microorganism into contact with a heat transfer unit having a temperature of more than 100° C. to carry out drying via heat transfer.2Paracoccus.. The production method according to claim 1 , wherein the carotenoid-producing microorganism is a microorganism of the genus3. The production method according to claim 1 , which comprises the step of further pulverizing the dried bacterial cell powder for fine powderization.4. The production method according to claim 3 , wherein the dried bacterial cell powder is pulverized to a volume particle size (D50) of 20 μm or less by the pulverization step.5. The production method according to claim 1 , wherein the temperature of the heat transfer unit is equal to or exceeds 120° C.6. The production method according to claim 1 , wherein the drying is drying carried out by using a drum dryer claim 1 , drying carried out by using a vacuum box dryer claim 1 , drying carried out by using a multi-cylinder dryer claim 1 , drying carried out by using a trough dryer claim 1 , drying carried out by using a shelf dryer claim 1 , or drying carried out by using a hot plate dryer.7. The production method according to claim 1 , wherein the carotenoid is astaxanthin.8. The production method according to claim 1 , wherein the produced powder is a powder for being added to a feed for poultry.9. A method for producing a feed for poultry claim 1 , said method comprising the step of adding a ...

Heterologous Carotenoid Production in Microorganisms

Non-naturally occurring microorganisms are provided that produce C40 carotenoid compound(s), utilizing exogenously added enzyme activities. Methods of producing C40 carotenoid compounds in microbial cultures, and feed and nutritional supplement compositions that include the C40 carotenoid compounds produced in the microbial cultures, are also provided. 1Paracoccus zeaxanthinifaciens, Escherichia vulnarisPantoea ananatis. A microorganism comprising a heterologous polynucleotide , comprising a polynucleotide sequence from , or that encodes a polypeptide of a C40 carotenoid biosynthetic pathway or comprising a polynucleotide sequence with at least about 70% sequence identity thereof or comprising a polynucleotide sequence that encodes a polypeptide comprising at least about 70% sequence identity to the polypeptide of the C40 carotenoid biosynthetic pathway , operably linked to a promoter for expression of said polynucleotide sequence ,wherein the microorganism is a bacterial cell from the class Alphaproteobacteria, andwherein the bacterial cell expresses said heterologous polynucleotide sequence to produce at least one C40 carotenoid compound.2Escherichia vulnerisEscherichia vulneris.. A microorganism according to claim 1 , further comprising a polynucleotide sequence that expresses the heterologous gene sequence idi from or comprising a polynucleotide sequence with at least about 70% sequence identity thereof or comprising a polynucleotide sequence that encodes a polypeptide comprising at least about 70% sequence identity to the polypeptide encoded by idi from3. A microorganism that is derived from a parent microorganism that expresses a native pathway for C30 carotenoid production claim 1 , wherein at least one gene sequence that encodes an enzyme of the native pathway for C30 carotenoid production has been disrupted or deleted such that C30 carotenoid production is reduced or eliminated in the microorganism in comparison to the parent microorganism from which it is ...

ASTAXANTHIN PRODUCTION USING A RECOMBINANT MICROBIAL HOST CELL

A recombinant microbial host cell is provided capable of producing astaxanthin from β-carotene without a measurable concomitant accumulation of ketolated or hydroxylated intermediates such as adonixanthin, zeaxanthin, adonirubin, echinenone, 3-hydroxyechinenone, 3′-hydroxyechinenone, canthaxanthin, and β-cryptoxanthin. Specifically, a β-carotene producing microbial host cell was engineered to express two heterologous genes, a β-carotene ketolase from in combination with a carotenoid hydroxylase from or 1. A recombinant microbial host cell comprising:a. a set of β-carotene biosynthesis pathway genes;{'i': 'Chlamydomonas reinhardtii', 'b. at least one expressible genetic construct encoding the β-carotene ketolase from ; and'}{'i': Brevundimonas', 'Arabidopsis thaliana, 'claim-text': 'wherein the recombinant microbial host cell produces astaxanthin from β-carotene and does not concomitantly accumulate a significant amount of any one of the following ketolated and/or hydroxylated carotenoid intermediates: adonixanthin, zeaxanthin, adonirubin, echinenone, 3-hydroxyechinenone, 3′-hydroxyechinenone, canthaxanthin or β-cryptoxanthin; wherein the ratio of astaxanthin to any one of the ketolasted and/or hydroxylated carotenoid intermediates as measured by dry cell weight is at least 75:1, preferably at least 100:1, more preferably at least 125:1, and most preferably at least 150:1.', 'c. at least one expressible genetic construct encoding a carotenoid hydroxylase selected from sp., or a combination thereof;'}2. The recombinant microbial host cell of claim 1 , wherein one or more of the set of β-carotene biosynthesis pathway genes present are foreign genes.3. The recombinant microbial host cell of claim 1 , where the set of β-carotene biosynthesis pathway genes are endogenous to the recombinant microbial host cell.4. The recombinant microbial host cell of claim 1 , or wherein the recombinant microbial host cell is a prokaryotic cell or eukaryotic cell.5. The recombinant ...

Duckweed Hydrolysate and use Thereof

A duckweed hydrolysate is provided. A method for producing carotenoids comprising the incubation of microorganisms with the duckweed is also provided. 19.-. (canceled)10. A method for producing a carotenoid comprising the steps of:incubating a carotenoid producing microorganism in a medium comprising an effective amount of a duckweed hydrolysate at suitable conditions;harvesting the cells; andisolating the carotenoid from the cells.11carotene, zeaxanthin, canthaxanthin, β-cryptoxanthin, astaxanthin, lycopenelutein.. The method according to claim 10 , wherein the carotenoid is selected from the group consisting of β- claim 10 , and12Escherichia coli, Saccharomyces cerevisiae, Blakeslea trispora, Agrobacterium aurantiacum, Haematococcus pluvialisXanthophyllomyces dendrorhous.. The method according to claim 10 , wherein the carotenoid producing microorganism is selected from the group consisting of claim 10 , and13E. coli.. The method according to claim 12 , wherein the carotenoid producing microorganism is1417.-. (canceled)18. The method according to claim 10 , wherein the duckweed hydrolysate is obtained by the process comprising the steps of: (a) obtaining duckweed juice from duckweed; (b) hydrolyzing the duckweed juice with one or more proteases at a temperature of about 25° C. to about 75° C. for about 6 to about 48 hours; and (c) collecting the duckweed hydrolysate. This Divisional Patent Application claims priority to U.S. Ser. No. 13/854,757, filed Apr. 1, 2013, entitled “Duckweed Hydrolysate and use Thereof,” the contents of which is incorporated by reference herein in its entirety.The invention relates to a hydrolysate product of duckweed, which can be used as a nutrient for cultivating microorganisms, such as , to increase the yields of cell biomass and cell products.Duckweed, comprising plants of the Lemnaceae family, is known to have fast doubling time and has been used as feed for ducks, chickens, fishes and shrimps. Duckweed can also be used to treat ...

COMBINED ELICITATION FOR ENHANCEMENT OF CELL GROWTH AND PRODUCTION OF SECONDARY METABOLITES IN MICROALGAE CULTURES

Methods of enhancing biomass and secondary metabolite accumulation of microalgal species are described herein. A cell culture of the microalgal species were elicited using a combination of techniques for a period of time. Experimental studies compared biomass dry weight production, chlorophyll dry weight content and astaxanthin dry weight content between controls and elicitation treatments. The present invention demonstrated that combined elicitation is an effective method for improving cell biomass growth and astaxanthin production. 1. A method of enhancing secondary metabolite accumulation in a microalgal cell culture , said method comprising:a) electrically eliciting the microalgal cell culture by exposing said culture to sub-lethal levels of electric current for a period of time during a stationary growth phase of the microalgal cell culture; andb) depriving the microalgal cell culture of nitrogen during the stationary growth phase, wherein combining electrical elicitation and nitrogen deprivation increases production of the secondary metabolite.2Haematococcus pluvialis. The method of claim 1 , wherein the microalgal cell culture comprises cells.3. The method of claim 2 , wherein the secondary metabolite is astaxanthin.4. The method of claim 1 , wherein electrical elicitation comprises placing electrodes in the microalgal cell culture claim 1 , wherein the electrodes are operatively coupled to a power source claim 1 , and the electrodes supply said sub-lethal levels of electric current.5. The method of claim 1 , wherein the sub-lethal levels of electric current ranges from about 30 mA to about 400 mA of direct current or alternating current.6. The method of claim 1 , wherein the period of time for electrical elicitation ranges from about 10 minutes to about 60 minutes.7. The method of further comprising mixing the microalgal cell culture during electrical elicitation to prevent ionic gradients.8. The method of further comprising incubating the microalgal cell ...

Heterotrophic Production Methods for Microbial Biomass and Bioproducts

The invention pertains to a method for synthesizing a product of interest by culturing a microalgal cell producing the product of interest in the dark in a culture medium comprising an organic acid as a fixed carbon source, wherein the microalgal cell is a facultative heterotroph. The product of interest can be a microalgal biomass, a pigment, terpene, recombinant molecule, biogas, or a precursor thereof. In an embodiment, the culture medium comprises urea as a primary source of nitrogen. In one embodiment, the microalgal cell belongs to the order Chlamydomonadales. A method of identifying and isolating a microalgal cell having a preferred characteristic that is suitable for synthesis of a product of interest is also provided, the method comprising identifying and isolating a non-mutagenized or recombinant microalgal cell from a microalgal culture using a fluorescence activated cell sorting technique and/or a phototaxic response. 1. An animal feed material or human food comprising non-encysted Chlamydomonadales microalgal cells or a purified product from said microalgal cells and produced according to a method , the method comprising:providing an organic acid as a carbon source into a culture medium;providing the microalgal cell that produces the feed material wherein the microalgal cell is a facultative heterotroph and is classified as part of the order Chlamydomonadales;culturing the microalgal cell in the culture medium in the dark to produce from the microalgal cell a microalgal culture comprising microalgal cells;isolating the microalgal cells from the microalgal culture after the cells reach a density of at least 6 g/L and before the cells form cyst cells; andpurifying the animal feed material or human food from the isolated microalgal cells or obtaining a microalgal biomass comprising the microalgal cells as the animal feed material or human food;whereby the animal feed material or human food comprising the non-encysted Chlamydomonadales microalgal cells or ...

USE OF THERMOPHILIC NUCLEASES FOR DEGRADING NUCLEIC ACIDS

The present invention relates to the use of a thermophilic nuclease for degrading nucleic acids in vivo and/or in situ, wherein the thermophilic nuclease is heterologous to the host cell and is produced by the host rather than being added exogenously. The present invention further relates to a genetically modified cell which was produced according to the above method. The present invention is particularly beneficial in inactivating the biological activity of recombinant DNA in biomass or biomass-derived products. 1. A genetically modified cell which comprises a heterologous nucleic acid sequence encoding a thermophilic nuclease gene , wherein the thermophilic nuclease produced by said thermophilic nuclease gene is latent at the temperature at which the cell has normal growth , wherein the genetically modified cell produces one or more end products , and wherein said one or more end products are not the thermophilic nuclease.2. The cell of claim 1 , wherein the nuclease is a DNA-degrading nuclease.3. The cell of claim 1 , wherein the nuclease is a RNA-degrading nuclease.4. The cell of claim 2 , wherein the DNA-degrading nuclease is TaqI nuclease.5. The cell of claim 2 , wherein the DNA-degrading nuclease is PhoI nuclease.6. The cell of any of - claim 2 , wherein the genetically modified cell is from a plant.7. The cell of any of - claim 2 , wherein the genetically modified cell is from an animal.8. The cell of any of - claim 2 , wherein the genetically modified cell is from a microorganism.9. The cell of claim 8 , wherein the microorganism is selected from a group consisting of: yeast claim 8 , fungi claim 8 , algae claim 8 , bacteria claim 8 , and archaea.10Yarrowia, Bacillus, Escherichia, Pseudomonas, Paracoccus, Corynebacterium, Candida, Hansenula, Saccharomyces, Mortierella, Schizosaccharomyces, Aspergillus, Fusarium, Trichoderma, Crypthecodinium, SchizochytriumThraustochytrium.. The cell of claim 9 , wherein the microorganism is selected from a group consisting ...

Methods for producing biomass rich in dha, palmitic acid and protein using a eukaryotic microorganism

Provided herein are eukaryotic microorganisms having a simple lipid profile comprising long chain fatty acids (LCFAs). Also provided are compositions and cultures comprising the eukaryotic microorganisms as well as methods of using the eukaryotic microorganisms.

CRISPR-CAS SYSTEM FOR A YEAST HOST CELL

The present invention relates to the field of molecular biology and cell biology. More specifically, the present invention relates to a CRISPR-CAS system for a yeast host cell. 1Saccharomyces cerevisiaeKluyveromyces lactis. A method of modulating expression of a polynucleotide in a cell , comprising contacting a host cell with a composition comprising a non-naturally occurring or engineered composition comprising a CRISPR-Cas system comprising a first guide-polynucleotide and a second guide-polynucleotide and a Cas protein , wherein the first and second guide-polynucleotides are distinct from each other , and wherein each comprises a guide-polynucleotide sequence that is the reverse complement of a target-polynucleotide sequence in a host cell , which host cell is or a , wherein each guide-polynucleotide directs binding of the Cas protein at the target-polynucleotide in the host cell to form a CRISPR-Cas complex , wherein each guide-polynucleotide sequence is the reverse complement of the (N)y part of a 5′-(N)yPAM-3′ polynucleotide sequence target in the genome of the host cell , wherein y is an integer of 8-30 , wherein PAM is a protospacer adjacent motif , wherein PAM is a sequence selected from the group consisting of 5′-XGG-3′ , 5′-XGGXG-3′ , 5′-XXAGAAW-3′ , 5′-XXXXGATT-3′ , 5′-XXAGAA-3′ , 5′-XAAAAC-3′ , wherein X can be any nucleotide; and W is A or T , wherein each guide-polynucleotide is encoded by a polynucleotide , and wherein the first and second polynucleotide encoding each guide-polynucleotide has sequence identity with a vector , and wherein each guide-polynucleotide-encoding polynucleotide has sequence identity with each other , and wherein the guide-polynucleotide directs binding of the Cas protein at the target-polynucleotide in the host cell to form a CRISPR-Cas complex.2. The method according to claim 1 , wherein the Cas protein is encoded by a polynucleotide.3. The method according to claim 1 , wherein the polynucleotides encoding the guide- ...

Productivity and Bioproduct Formation in Phototropin Knock/Out Mutants in Microalgae

Phototropin is a blue light receptor, which mediates a variety of blue-light elicited physiological processes in plants and algae. In higher plants these processes include phototropism, chloroplast movement and stomatal opening. In the green alga , phototropin plays a vital role in progression of the sexual life cycle and in the control of the eye spot size and light sensitivity Phototropin is also involved in blue-light mediated changes in the synthesis of chlorophylls, carotenoids, chlorophyll binding proteins. We compared the transcriptome of phototropin knock out (PHOT KO) mutant and wild-type parent to analyze differences in gene expression in high light grown cultures (500 μmol photons ms). Our results indicate the up-regulation of genes involved in photosynthetic electron transport chain, carbon fixation pathway, starch, lipid, and cell cycle control genes. With respect to photosynthetic electron transport genes, genes encoding proteins of the cytochrome b6f and ATP synthase complex were up regulated potentially facilitating proton-coupled electron transfer. In addition genes involved in limiting steps in the Calvin cycle Ribulose-1,5-bisphosphate carboxylase/oxygenase (RuBisCO), Sidoheptulose 1,7 bisphosphatase (SBPase), Glyceraldehyde-3-phosphate dehydrogenase (3PGDH) and that mediate cell-cycle control (CDK) were also up regulated along with starch synthase and fatty acid biosynthesis genes involved in starch and lipid synthesis. In addition, transmission electron micrographs show increased accumulation of starch granules in PHOT mutant compared to wild type, which is consistent with the higher expression of starch synthase genes. Collectively, the altered patterns of gene expression in the PHOT mutants were associated with a two-fold increase in growth and biomass accumulation compared to wild type when grown in environmental photobioreactors (Phenometrics) that simulate a pond environment. In conclusion, our studies suggest that phototropin may be a ...

Method For Producing Carotenoids Each Having 50 Carbon Atoms

The present invention provides a method of producing a carotenoid having 50 carbon atoms which comprises culturing, in a medium, a cell transformed with a mutant phytoene desaturase gene and obtaining the carotenoid having 50 carbon atoms from the culture. The mutant phytoene desaturase gene has an introduced mutation to encode a mutant phytoene desaturase having an enhanced activity to desaturate a carotenoid backbone compound of 50 carbon atoms. 115-. (canceled)16. A method of producing a carotenoid having 55 carbon atoms , comprising: culturing , in a medium , a cell transformed with a mutant diapophytoene synthase gene and a mutant farnesyl diphosphate synthase gene; andobtaining the carotenoid having 55 carbon atoms from a culture after the culturing,wherein the transformation is performed by a plasmid having a nucleotide sequence of SEQ ID NO: 26 that contains the mutant diapophytoene synthase gene and a plasmid having a nucleotide sequence of SEQ ID NO: 25 that contains the mutant farnesyl diphosphate synthase gene.17. A method of producing a carotenoid having 60 carbon atoms , comprising: culturing , in a medium , a cell transformed with a mutant diapophytoene synthase gene and a mutant hexaprenyl diphosphate synthase gene; andobtaining the carotenoid having 60 carbon atoms from a culture after the culturing,wherein the transformation is performed by a plasmid having a nucleotide sequence of SEQ ID NO: 26 that contains the mutant diapophytoene synthase gene and a plasmid having a nucleotide sequence of SEQ ID NO: 24 that contains the mutant farnesyl diphosphate synthase gene. This application is a continuation of U.S. patent application Ser. No. 14/124,256, filed Dec. 6, 2013, which is a National Stage Application of PCT/JP2012/064817, filed Jun. 8, 2012, which claims priority from Japanese Patent Application No. 2011-130326, filed Jun. 10, 2011.The present invention relates to a production method for a carotenoid having 50 carbon atoms, including the step ...

CAROTENOID AND AMINO ACID BIOSYNTHESIS USING RECOMBINANT CORYNEBACTERIUM GLUTAMICUM

The present invention provides a method of producing astaxanthin and lysine in recombinant gram-positive bacteria comprising a nucleic acid sequence encoding for a crtZ-protein from and comprises a nucleic acid sequence encoding for a crtW-protein. 1C. glutamicumC. glutamicumC. glutamicumF. pelagiF. pelagiB. aurantiacaS. astaxanthinifaciens. A process for the preparation of astaxanthin and lysine in recombinant , wherein in the genome of said recombinant crtR , crtY from and crtEb were deleted and crtEBI , crtYand at least one recombinant sequence which comprises a nucleic acid sequence encoding for a crtZ-protein (crtZ-nucleic acid sequence) , preferably from (crtZ-nucleic acid sequence) and at least one recombinant sequence which comprises a nucleic acid sequence encoding for a crtW-protein (crtW-nucleic acid sequence) , preferably from (crtW-nucleic acid sequence) , (crtW-nucleic acid sequence) or (crtW-nucleic acid sequence) were introduced.2. The process according to claim 1 , wherein the crtZ-nucleic acid sequence is SEQ ID NO.: 1 claim 1 , ora nucleic acid sequence having at least 80% identity as set forth with SEQ ID NO.: 1, ora nucleic acid sequence that hybridizes with the complement of a nucleic acid sequence according to SEQ ID NO.: 1 under the following hybridization conditions: 0.1×SSC, 0.1% SDS, 65° C. and wash conditions 2×SSC, 0.1% SDS, 65° C., followed by 0.1×SSC, 0.1% SDS, 65° C. (high stringency conditions), ora nucleic acid sequence encoding for an amino acid sequence having at least 80% identity with SEQ ID NO.: 2 and which amino acid sequence shows crtZ activity.3. The process according to claim 2 , wherein the crtZ-nucleic acid sequence is SEQ ID NO.: 1.4. The process according to or claim 2 , wherein the crtW-nucleic acid sequence is SEQ ID NO.: 3 or SEQ ID NO.: 5 or isa nucleic acid sequence having at least 80% identity as set forth with SEQ ID NO.: 3 or 5, respectively, ora nucleic acid sequence that hybridizes with the complement of a ...

High concentration methanol tolerant methanotroph and its application

The present invention discloses a high concentration methanol tolerant methanotroph and its application, a accession number of the methanotroph in China General Microbiological Culture Collection Center being CGMCC No. 9873, deposit date being Oct. 29, 2014, category names being sp. ZR1. The methanotroph sp. ZR1 disclosed by the present invention can grow rapidly by using methane, and can tolerate with high concentration of methanol. The methanotroph sp. ZR1 can use Ccompounds such as methane and methanol to produce high value-added products such as carotenoids and polysaccharides, which has high application prospect in biological transformation of one-carbon chemistry. 1Methylomonas. A high concentration methanol tolerant methanotroph named sp. ZR1 , characterized by , an accession number of the methanotroph in China General Microbiological Culture Collection Center being CGMCC No. 9873 , deposit date of the methanotroph being Oct. 29 , 2014.2. An application of the high concentration methanol tolerant methanotroph of claim 1 , which is for producing carotenoids.3. The application of claim 2 , characterized by claim 2 , the carotenoids being produced by fermentation product which is obtained from inoculating and fermenting the methanotroph with methane or methanol as substrate in a fermentation temperature of 20˜30° C.4. The application of claim 3 , characterized by claim 3 , the fermentation temperature being 25° C.5. The application of claim 4 , characterized by claim 4 , wherein when the methanol is used as the substrate claim 4 , the mass percent concentration of the methanol in the fermentation culture medium is less than or equal to 3.5%.6. An application of the high concentration methanol tolerant methanotroph of claim 1 , which is for producing polysaccharide.7. The application of claim 6 , characterized by claim 6 , polysaccharides being produced by the fermentation product claim 6 , which is obtained from inoculating and fermenting of the methanotroph ...

METHODS OF PRODUCTION OF PRODUCTS OF METABOLIC PATHWAYS

A plurality of isolated polynucleotide sequences encoding enzymes of the astaxanthin pathway is disclosed. The polynucleotides include: 1. A plurality of isolated polynucleotide sequences encoding enzymes of the astaxanthin pathway comprising:(i) a polynucleotide which encodes Phytoene dehydrogenase (crtI) and a first transcriptional regulatory sequence;(ii) a polynucleotide which encodes Beta-lycopene cyclase (lcy-B) and a second transcriptional regulatory sequence;(iii) a polynucleotide which encodes Beta-carotene ketolase (crtW) and a third transcriptional regulatory sequence; andwherein said first, second and third regulatory sequence are selected such that the expression of said lcy-B and said crtW is greater than a level of expression of said crtI.2. The plurality of isolated polynucleotide sequences of claim 1 , further comprising at least one of:(iv) a polynucleotide which encodes Isopentenyl pyrophosphate (idi) and a fourth transcriptional regulatory sequence; or(v) a polynucleotide which encodes Geranylgeranyl pyrophosphate synthase (crtE) and a fifth transcriptional regulatory sequence; or(vi) a polynucleotide which encodes Prephytoene pyrophosphate synthase (crtB) and a sixth transcriptional regulatory sequence; or(vii) a polynucleotide which encodes Beta-carotene hydroxylase (crtZ) and a seventh transcriptional regulatory sequence.3. A plurality of isolated polynucleotide sequences encoding enzymes of the astaxanthin pathway comprising:(i) a polynucleotide which encodes Isopentenyl pyrophosphate (idi) and a first transcriptional regulatory sequence;(ii) a polynucleotide which encodes Geranylgeranyl pyrophosphate synthase (crtE) and a second transcriptional regulatory sequence;(iii) a polynucleotide which encodes Prephytoene pyrophosphate synthase (crtB) and a third transcriptional regulatory sequence;(iv) a polynucleotide which encodes Phytoene dehydrogenase (crtI) and a fourth transcriptional regulatory sequence;(v) a polynucleotide which encodes Beta- ...

Method for culturing photosynthetic microalgae

The present invention provides a method for culturing photosynthetic microalgae with which xanthophyll can be obtained more efficiently than before. The culture method of the present invention comprises a step of performing light irradiation of encysted photosynthetic microalgae containing xanthophyll in an amount of 3 to 9% by mass in terms of a dry mass. Preferably, in the step of performing light irradiation, the xanthophyll content in photosynthetic microalgae is kept at 2% by mass or more in terms of a dry mass. Preferably, the step of performing light irradiation includes step (A) of increasing the number of cells in which light irradiation (a) is performed; and step (B) of increasing the xanthophyll content in photosynthetic microalgae in which light irradiation (b) of the photosynthetic microalgae subjected to the step (A) of increasing the number of cells is performed.

Recombinant microorganism for producing crocin and method for producing crocin using the same

Compared with the conventional method for producing crocin, which is produced in small amounts through a part of plants or callus, the production method using the recombinant microorganism of the present disclosure enables mass production of crocin.

CAROTENOIDS FOR TREATING OR PREVENTING NAUSEA

Methods and compositions comprising carotenoids for the treatment or prevention of nausea, e.g., chemotherapy-induced nausea and vomiting. 174.-. (canceled)75. A therapeutic composition formulated for oral delivery comprising a therapeutically effective amount of a C50 carotenoid compound , and a pharmaceutically acceptable carrier , wherein the composition comprises a microbe that synthesizes the C50 carotenoid compound.76. The therapeutic composition of claim 75 , wherein the microbe is a cultured microbe.77. The therapeutic composition of claim 76 , wherein the microbe is an engineered microbe.78. The therapeutic composition of claim 77 , wherein the engineered microbe comprises a genetic alteration relative to an otherwise comparable reference microbe so that it produces the C50 carotenoid compound at an absolute or relative level different from that of the reference microbe.79. The therapeutic composition of claim 75 , which is a liquid claim 75 , syrup claim 75 , tablet claim 75 , troche claim 75 , gummy claim 75 , capsule claim 75 , powder claim 75 , gel claim 75 , or film.80. The therapeutic composition of claim 75 , wherein the C50 carotenoid compound is at least 20% w/w of the composition.81. The therapeutic composition of claim 75 , wherein the C50 carotenoid compound is purified.82. The therapeutic composition of claim 75 , wherein the C50 carotenoid compound is an analog of a reference C50 carotenoid compound found in nature.83. The therapeutic composition of claim 75 , wherein the C50 carotenoid compound is selected from the group consisting of decaprenoxanthin claim 75 , C50-astaxanthin claim 75 , C50-β-carotene claim 75 , C50-carotene (n=3) (16 claim 75 ,16-diisopentenylphytoene) claim 75 , C50-zeaxanthin claim 75 , C50-caloxanthin claim 75 , C50-nostoxanthin sarcinaxanthin claim 75 , sarprenoxanthin claim 75 , acyclic C50 carotenoid bacterioruberin claim 75 , C50-canthaxanthin claim 75 , C50-lycopene claim 75 , C50-phytoene claim 75 , and ...

Duckweed Hydrolysate and use Thereof

A duckweed hydrolysate is provided. A method for producing carotenoids comprising the incubation of microorganisms with the duckweed is also provided. 1. A duckweed hydrolysate , which is obtained by the process comprising the steps of:(a) obtaining duckweed juice from duckweed;(b) hydrolyzing the duckweed juice with one or more proteases at a temperature of about 25° C. to about 75° C. for about 6 to about 48 hours; and(c) collecting the duckweed hydrolysate.2Lemna aequinoctialis.. The duckweed hydrolysate according to claim 1 , wherein the duckweed is3Lemna aequinoctialis.. The duckweed hydrolysate according to claim 2 , wherein the duckweed is4. The duckweed hydrolysate according to claim 1 , wherein the one or more proteases are endoproteases.5. The duckweed hydrolysate according to claim 4 , wherein the protease is PROTAMEX®.6. The duckweed hydrolysate according to claim 1 , wherein the one or more proteases are in an amount of about 3 A.U. to about 15 A.U. per liter of the duckweed juice.7. The duckweed hydrolysate according to claim 1 , wherein the temperature in step (b) is about 35° C. to about 45° C.8. The duckweed hydrolysate according to claim 1 , wherein the reaction time in step (b) is about 12 to about 24 hours.9. The duckweed hydrolysate according to claim 1 , wherein the process further comprises step (e) where the duckweed hydrolysate collected from step (d) is concentrated and/or dried so that a concentrated and/or dried duckweed hydrolysate is obtained.10. A method for producing a carotenoid comprising the steps of: incubating a carotenoid producing microorganism in a medium comprising an effective amount of the duckweed hydrolysate according to at suitable conditions; harvesting the cells; and isolating the carotenoid from the cells.11. The method according to claim 10 , wherein the carotenoid is selected from the group consisting of β-carotene claim 10 , zeaxanthin claim 10 , canthaxanthin claim 10 , β-cryptoxanthin claim 10 , astaxanthin claim ...

IN VIVO PRODUCTION OF A RECOMBINANT CAROTENOID-PROTEIN COMPLEX

The present invention relates to a method for producing a carotenoid-protein complex in vivo comprising the steps of transforming of prokaryote cell with genes involved in carotenoid synthesis and with a gene encoding an apo-carotenoprotein; culturing said prokaryote cells such as to induce sequential genes expression, isolating and purifying the carotenoid-protein complex. 1. A method for producing a carotenoid-protein complex in vivo comprising the steps of:a) transforming of prokaryote cells with genes involved in carotenoid synthesis and a gene encoding an apo-carotenoprotein;b) culturing of transformed prokaryote cells in step a) in conditions allowing sequential gene expression, wherein the expression of the genes involved in carotenoid synthesis is induced prior to the expression of the gene encoding an apo-carotenoproteinc) isolating and purifying the carotenoid-protein complex expressed by the prokaryote cells.2. The method according to wherein the prokaryote cell in step a) is transformed with at least one plasmid containing the genes involved in carotenoid synthesis and with one plasmid containing a gene encoding an apo-carotenoprotein claim 1 , wherein said plasmids contain different replication origins and different selective pressure.3. The method according to wherein the at least one plasmid containing the genes involved in carotenoid synthesis in step a) is a plasmid containing the genes involved in β-carotene synthesis4. The method according wherein the transforming of step a) further comprises a second plasmid containing the genes encoding a β-carotene ketolase and/or the genes encoding a β-carotene hydrolase.5. The method according claim 1 , where in the plasmid containing the genes involved in β-carotene synthesis further contains the genes encoding a β-carotene ketolase and/or the genes encoding β-carotene hydrolase under the control of an inducible promoter claim 1 , preferably ara promoter.6. The method of claim 1 , wherein in step a) the ...

METHOD FOR PRODUCING COENZYME Q10

A method of producing coenzyme Q10 includes contacting an extract from a coenzyme Q10-producing microorganism with an adsorbent (A) such that the adsorbent (A) adsorbs a component of the extract other than coenzyme Q10, and that coenzyme Q10 is obtained. The adsorbent (A) includes aluminum silicate at a content of 50% or more. 1. A method of producing coenzyme Q10 , comprising:contacting an extract from a coenzyme Q10-producing microorganism with an adsorbent (A) such that the adsorbent (A) adsorbs a component of the extract other than coenzyme Q10, and that coenzyme Q10 is obtained,wherein the adsorbent (A) includes aluminum silicate at a content of 50% or more.2. The method of claim 1 , further comprising:contacting the extract with an adsorbent (B).3. The method of claim 1 , wherein the contacting comprises contacting the extract with a mixture including the adsorbent (A) and an adsorbent (B).4. The method of claim 2 , wherein the adsorbent (B) comprises at least one selected from the group consisting of activated carbon claim 2 , an adsorbent including activated carbon as a main component claim 2 , a synthetic adsorbent obtained by copolymerizing styrene and divinylbenzene claim 2 , a phenol-formaldehyde resin adsorbent claim 2 , an aldehyde adsorbent claim 2 , magnesium silicate claim 2 , aluminum hydroxide claim 2 , aluminum oxide claim 2 , magnesium oxide claim 2 , diatomite claim 2 , activated alumina claim 2 , silica gel claim 2 , silica-magnesia gel claim 2 , an adsorbent obtained by chemically binding an alkyl group or an allyl group on an inorganic carrier claim 2 , an aromatic adsorbent claim 2 , a methacrylate ester adsorbent claim 2 , synthetic zeolite claim 2 , and a pearlite filter medium.5. The method of claim 3 , wherein the adsorbent (B) comprises at least one selected from the group consisting of activated carbon claim 3 , an adsorbent including activated carbon as a main component claim 3 , a synthetic adsorbent obtained by copolymerizing ...

NOVEL VIOLAXANTHIN-OVERPRODUCING STRAIN OF CHLORELLA VULGARIS AND THE METHOD FOR PRODUCING VIOLAXANTHIN USING THE SAME

The present invention relates to a novel violaxanthin-overproducing strain of and a method of producing violaxanthin therefrom. The inventors have developed a strain that produces violaxanthin at a significantly higher level than a wild-type strain by inducing a random chemical mutation in a strain to, and then as a result of analysis, confirmed that the strain produces violaxanthin up to 0.41% based on dry weight, which reaches the highest level that is possible to be produced in microalgae. Furthermore, as a method of effectively extracting a carotenoid pigment containing violaxanthin from the strain was established, since the strain and the developed pigment extraction method according to the present invention allow effective production and separation of violaxanthin, the strain is expected to increase commercial applications such as cosmetics, health functional foods and feed. 1Chlorella vulgaris. A violaxanthin-overproducing CvLD-01 strain (Accession No. KCTC 14091BP).2Chlorella vulgaris. The strain of claim 1 , wherein the strain is derived from a UTEX395 strain (Accession No. KCTC 14091BP).3. The strain of claim 1 , wherein the strain has alanine (A) claim 1 , which is amino acid 336 of the lycopene epsilon cyclase (CvLCYE) gene claim 1 , substituted with valine (V) (Accession No. KCTC 14091BP).4Chlorella vulgaris. A method of extracting carotenoid pigment from the strain of using glass bead-added sonication.5. The method of claim 4 , wherein the extraction method uses methanol as a solvent.6. The method of claim 4 , wherein the glass bead has a diameter of 0.4 to 0.7 mm.7Chlorella vulgaris. A method of producing violaxanthin on a large scale claim 1 , which comprises culturing the CvLD-01 strain of .8. The method of claim 7 , wherein the culturing is performed for 72 to 96 hours in a medium containing acetic acid as a carbon source under 100 μmol photon ms.9Chlorella vulgaris. A composition for food or a food additive claim 1 , comprising one or more ...

DRIED BACTERIAL CELL POWDER CONTAINING A CAROTENOID AND METHOD FOR PRODUCING THE SAME

According to the present invention, a powder containing a carotenoid for feed having improved color enhancing ability, and a method for producing the same are provided. A method for producing a dried bacterial cell powder containing a carotenoid, comprising a step of drying via conductive heat transfer and a pulverization step and a dried bacterial cell powder produced by the method are provided. 1. A method for producing a dried bacterial cell powder containing astaxanthin , said method comprising the steps of:{'i': 'Paracoccus', '(a) contacting bacterial cells of an astaxanthin-producing microorganism of the genus with a heat transfer unit having a temperature of more than 100° C. to carry out drying via heat transfer thereby producing a dried bacterial cell powder; and'}(b) pulverizing the dried bacterial cell powder of step (a) for fine powderization, wherein the volume particle size (D50) of the dried bacterial cell powder is pulverized to 20 μm or less by the pulverizing step.25.-. (canceled)6. The production method according to claim 1 , wherein the drying is drying carried out by using a drum dryer claim 1 , drying carried out by using a vacuum box dryer claim 1 , drying carried out by using a multi-cylinder dryer claim 1 , drying carried out by using a trough dryer claim 1 , drying carried out by using a shelf dryer claim 1 , or drying carried out by using a hot plate dryer.7. (canceled)8. The production method according to claim 1 , wherein the produced powder is a powder for being added to a feed for poultry.9. A method for producing a feed for poultry claim 1 , said method comprising the step of adding the dried bacterial cell powder produced by the method according to to a feed for poultry.1013.-. (canceled) The present invention relates to a dried bacterial cell powder containing a carotenoid and a method for producing the same.Carotenoid compounds have been added to feed (feedstuff) for the purpose of enhancing chicken egg yolk coloration or the red ...

TROPHIC CONVERSION OF PHOTOAUTOTROPHIC BACTERIA FOR IMPROVED DIURNAL PROPERTIES

The present disclosure relates generally to the growth of recombinant bacterial cells of photoautotrophic species under diurnal conditions. In particular, the present disclosure relates to isolated bacterial cells of photoautotrophic species having increased growth under diurnal conditions by expression of a sugar transporter protein and methods of use thereof. 1. An isolated bacterial cell of a photoautotrophic species , comprising a recombinant polynucleotide encoding a galactose transporter protein , wherein expression of the galactose transporter protein results in transport of glucose into the bacterial cell to increase growth of the bacterial cell on glucose under dark or diurnal conditions as compared to a corresponding photoautotrophic bacterial cell lacking the recombinant polynucleotide.2. The isolated bacterial cell of claim 1 , wherein the recombinant polynucleotide encodes a galactose transporter protein selected from the group consisting of a bacterial galP transporter protein claim 1 , a eukaryotic galP transporter protein claim 1 , a fungal galP transporter protein claim 1 , a mammalian galP transporter protein claim 1 , a bacterial Major Facilitator Superfamily (MFS) transporter protein claim 1 , a eukaryotic MFS transporter protein claim 1 , a fungal MFS transporter protein claim 1 , a mammalian MFS transporter protein claim 1 , a bacterial ATP-Binding Cassette Superfamily (ABC) transporter protein claim 1 , a eukaryotic ABC transporter protein claim 1 , a fungal ABC transporter protein claim 1 , a mammalian ABC transporter protein claim 1 , a bacterial Phosphotransferase System (PTS) transporter protein claim 1 , a eukaryotic PTS transporter protein claim 1 , a fungal PTS transporter protein claim 1 , a mammalian PTS transporter protein claim 1 , and a homolog thereof.3E. coli. The isolated bacterial cell of claim 1 , wherein the recombinant polynucleotide encodes an galP transporter protein.4. An isolated bacterial cell of a photoautotrophic ...

Modified Cyanobacteria for Producing Carotenoids

This disclosure describes modified photosynthetic microorganisms, including Cyanobacteria that produce carotenoids, including zeaxanthin, astaxanthin, and/or canthaxanthin. The modifications include one or more genetic modifications such as gene deletion, up regulation of an endogenous gene, and/or addition of an exogenous gene. In some embodiments the modified photosynthetic microorganisms may be subjected to stress conditions. 1. A method of producing a carotenoid , the method comprising:culturing a modified Cyanobacterium, the modified Cyanobacterium having decreased glycogen production; andsubjecting the modified Cyanobacterium to a stress condition during culturing, wherein the modified Cyanobacterium when subjected to the stress condition produces the carotenoid at a level greater than the corresponding wild-type Cyanobacterium subject to the same stress condition.2. (canceled)3. The method of claim 1 , wherein the carotenoid comprises zeaxanthin claim 1 , astaxanthin claim 1 , canthaxanthin claim 1 , or a combination thereof.46-. (canceled)7. The method of claim 1 , wherein the modified Cyanobacterium is modified by one of (i) deletion or inactivation of a glycogen pathway gene or (ii) by addition of a regulatable claim 1 , exogenous promoter that controls transcription of a glycogen pathway gene.8. (canceled)9. The method of claim 7 , wherein the regulatable claim 7 , exogenous promoter claim 7 , when activated by a regulator claim 7 , controls transcription of the glycogen pathway gene claim 7 , the regulator comprises a nutrient claim 7 , and the stress condition comprises deprivation of the nutrient.10. The method of claim 7 , wherein the glycogen pathway gene is glgC.11. The method of claim 1 , wherein the stress condition comprises at least one of high light claim 1 , high salt claim 1 , nutrient deprivation claim 1 , nitrogen deprivation claim 1 , sulfur deprivation claim 1 , phosphorous deprivation claim 1 , or iron deprivation.1214-. (canceled)15. ...

Method for enhancement of productivity in microalgae

The instant disclosure relates to the field of algal cultivation and production of high value biochemical products thereof. Particularly, the present disclosure relates to a cultivation method comprising the application of red light/far-red light in life cycle management of green microalgae, particularly Haematococcus , including induction of intense vegetative growth, and enhancement of productivity. The present method is simple, commercially scalable and cost-effective, achieves enhanced productivity, and requires shorter time duration, amongst other advantages.

GENES INVOLVED IN ASTAXANTHIN BIOSYNTHESIS

Provided herein are nucleic acid, host cell, and polypeptide compositions encoded by the unicellular green alga , methods of making such compositions, and method of using the compositions to produce astaxanthin. 1. An expression vector comprising a nucleic acid sequence that encodes a polypeptide involved in astaxanthin production , wherein the polypeptide has at least 90% identity to any one of SEQ ID NOS: 1 to 7.2. The expression vector of claim 1 , wherein the polypeptide comprises the amino acid sequence of any one of SEQ ID NOS:1 to 7.3Chloroformis zofingiensis. The expression vector of claim 1 , wherein the nucleic acid sequence is a cDNA or genomic DNA sequence from a gene Cz04g21110 claim 1 , Cz05g17060 claim 1 , Cz09g27180 claim 1 , Cz08g16130 claim 1 , Cz10g28330 claim 1 , Cz11g14160 claim 1 , Cz02g29020.4. The expression vector of claim 1 , wherein the nucleic acid sequence encodes an ABC transporter.5Chloroformis zofingiensis. The expression vector of claim 4 , wherein the nucleic acid sequence is a cDNA or genomic sequence from a gene Cz04g21110 claim 4 , Cz05g17060 claim 4 , Cz09g27180 claim 4 , or Cz08g16130.6. The expression vector of claim 1 , wherein the nucleic acid sequence encodes a cytochrome P450 protein.7Chloroformis zofingiensis. The expression vector of claim 6 , wherein the nucleic acid sequence is a cDNA or genomic sequence from a gene Cz10g28330 or Cz11g14160.8. The expression vector of claim 1 , wherein the nucleic acid encodes an acyltransferase.9Chloroformis zofingiensis. The expression vector of claim 8 , wherein the nucleic acid sequence is a cDNA or genomic sequence from a gene Cz02g29020.10. The expression vector of claim 1 , wherein the nucleic acid sequence has at least 70% identity to any one of SEQ ID NOS:8 to 21.11. The expression vector of claim 1 , wherein the nucleic acid sequence has at least 70% identity to any one of SEQ ID NOS:15 to 21.12. The expression vector of claim 1 , wherein the nucleic acid sequence has at ...

A method of culturing haematococcus species for manufacturing of astaxanthin

A method of culturing Haematococcus species for manufacturing of astaxanthin comprising the steps of: providing a substrate, arranging the Haematococcus species on the surface of the substrate, exposing the Haematococcus species arranged on the substrate to high light intensities from the beginning of a culturing process and avoiding a two-step culturing process of the Haematococcus species with a first step which is an initial culturing taking place by exposure of the Haematococcus species to low light energy followed by a second step of subsequent culturing of the Haematococcus species by exposure of the Haematococcus species to higher light energy than applied in the first step to induce astaxanthin formation, and optionally harvesting the cultured Haematococcus species and/or isolating astaxanthin.

PRODUCTION OF RETINYL ESTERS

The present invention is related to a novel enzymatic process for production of retinyl esters, such as in particular retinyl long chain esters, via conversion of retinol, which process includes the use of enzymes having acyltransferase activity. Said process might be used for biotechnological production of vitamin A. 1. A carotenoid-producing host cell comprising an enzyme with retinol acylating activity , preferably acyltransferase [EC 2.3.1] activity , more preferably acyltransferase [EC 2.3.1.20] activity , said host cell producing long chain retinyl esters , with a percentage of at least about 20% based on the total amount of retinoids produced by said host cell.2. The carotenoid-producing host cell according to comprising a heterologous acyltransferase.3Yarrowia lipolyticaSaccharomyces cerevisiae.. The carotenoid-producing host cell according to claim 1 , wherein the host cell is selected from plants claim 1 , fungi claim 1 , algae or microorganisms claim 1 , preferably selected from the group consisting of Escherichia claim 1 , Streptomyces claim 1 , Pantoea claim 1 , Bacillus claim 1 , Flavobacterium claim 1 , Synechococcus claim 1 , Lactobacillus claim 1 , Corynebacterium claim 1 , Micrococcus claim 1 , Mixococcus claim 1 , Brevibacterium claim 1 , Bradyrhizobium claim 1 , Gordonia claim 1 , Dietzia claim 1 , Muricauda claim 1 , Sphingomonas claim 1 , Synochocystis claim 1 , Paracoccus claim 1 , Saccharomyces claim 1 , Aspergillus claim 1 , Pichia claim 1 , Hansenula claim 1 , Phycomyces claim 1 , Mucor claim 1 , Rhodotorula claim 1 , Sporobolomyces claim 1 , Xanthophyllomyces claim 1 , Phaffia claim 1 , Blakeslea and Yarrowia claim 1 , more preferably from or4Yarrowia.. The carotenoid-producing host cell according to claim 1 , wherein the acyltransferase is selected from plants claim 1 , animals claim 1 , including humans claim 1 , algae claim 1 , fungi claim 1 , including yeast claim 1 , or bacteria claim 1 , preferably selected from the group consisting ...

PRODUCTION OF RETINYL ESTERS

The present invention is related to production of retinyl esters, such as in particular retinyl acetate, an important building block for production of vitamin A. The retinyl esters might be generated via enzymatic conversion of retinol which process includes the use of enzymes with acetyl-transferase (ATF) activity, thus acetylating retinol into retinol/retinyl acetate. Said process is particularly useful for biotechnological production of vitamin A. 1. A carotenoid-producing host cell comprising an enzyme with acetylating activity , such as retinol acetylating activity , preferably acetyl transferase [EC 2.3.1.84] activity , more preferably acetyl transferase 1 activity , said host cell producing a retinyl ester mix with a conversion rate of retinol to retinyl esters of at least 10%.2. The carotenoid-producing host cell of comprising an enzyme with retinol acetylating activity with preference for acetylation of trans-retinol.3. The carotenoid-producing host cell of claim 1 , wherein the retinyl ester is selected from retinyl acetate.4. The carotenoid-producing host cell according comprising a heterologous acetyl transferase claim 1 , preferably heterologous acetyl transferase 1.5Petunia, Euonymus, Escherichia, SaccharomycesLachancea.. The carotenoid-producing host cell according to claim 1 , wherein the acetyl transferase claim 1 , preferably acetyl transferase 1 claim 1 , is selected from plants claim 1 , animals claim 1 , including humans claim 1 , algae claim 1 , fungi claim 1 , including yeast claim 1 , or bacteria claim 1 , preferably selected from the group consisting of claim 1 , and6Petunia hybrida, Euonymus alatus, Saccharomyces bayanus, Saccharomyces kudriavzevii, Saccharomyces arbirocola, Lachancea mirantinaLachancea fermentati.. The carotenoid-producing host cell according to claim 5 , wherein the acetyl transferase is acetyl transferase 1 selected from claim 5 , or7. The carotenoid-producing host cell according to claim 6 , wherein the acetyl ...

METHOD OF FERMENTATIVE ALPHA-IONONE PRODUCTION

The present invention concerns a method of producing and enantiomerically pure alpha-ionone. Further, the invention concerns a nucleic acid that comprises a sequence that encodes a lycopene-epsilon-cyclase (EC), a lycopene-epsilon-cyclase (EC), plasmids, which encode components of the alpha-ionone biosynthesis and a microorganism that contains heterologous nucleotide sequences which encode the enzymes geranylgeranyl-diphosphate-synthase, isopentenyl-diphosphate-isomerase (IPI), phytoene desaturase-dehydrogenase (crtI), phytoene synthase (crtB), lycopene-epsilon-cyclase (EC) and carotenoid-cleavage-dioxygenase (CCD1). Further, the invention concerns a method of producing highly pure epsilon-carotene. 1. A method of producing enantiomerically pure alpha-ionone comprising culturing a microorganism that comprises heterologous nucleotide sequences that encode the following enzymes:a. geranylgeranyl-diphosphate-synthase,b. isopentenyl-diphosphate-isomerase (ipi),c. phytoene-desaturase/dehydrogenase (crtI),d. phytoene synthase (crtB),e. lycopene-epsilon-cyclase (EC) andf. carotenoid-cleavage-dioxygenase (CCD1).2. The method according to claim 1 , wherein the geranylgeranyl-diphosphate-synthase is the geranylgeranyl-diphosphate-synthase crtE for the geranylgeranyl-diphosphate-synthase idsA.3. The method according to or claim 1 , wherein the lycopene-epsilon-cyclase (EC) has at least 80% sequence identity with a sequence according to SEQ ID NO. 19 and deviates at least at one position of 403 claim 1 , 404 and 445 from the sequence according to SEQ ID NO. 19.4. The method according to one of to claim 1 , wherein the lycopene-epsilon-cyclase (EC) comprises one of the following mutations or mutation combinations: ECmut9 (L404S) claim 1 , ECmut10 (A403S/L404T) claim 1 , ECmut3.3 (A403E/L404A/A445S) and ECmut3.2 (A403C/L404C/A445S).5Arabidopsis thalianaOsmanthus fragans.. The method according to one of to claim 1 , wherein the carotenoid-cleavage-dioxygenase (CCD1) is a ...

BACTERIA AND THE USES THEREOF

The present invention relates to novel bacteria and the uses thereof. The invention particularly relates to bacteria having a metabolic pathway ratio between Pentose phosphate and glycolysis greater than 0.5, and their uses in the chemical, pharmaceutical or agro-chemical industries, e.g., for producing compounds of industrial interest. 125-. (canceled)26. An isolated bacterium , wherein said bacterium has a metabolic pathway ratio between Pentose phosphate and glycolysis greater than 0.5.27. The bacterium of claim 26 , wherein the metabolic pathway ratio between Pentose phosphate and glycolysis is:a) equal to or greater than 1;b) between 1 and 20; orc) between 2 and 20.28Deinococcus. The bacterium of claim 26 , wherein said bacterium is a or related bacterium.29. The bacterium of claim 26 , said bacterium being obtained by a method comprising the following steps:a) providing a sample comprising bacteria;b) subjecting the sample to a cell-destructing DNA damaging treatment;c) identifying or isolating living or growing bacteria from said treated sample;d) measuring the metabolic pathway ratio between Pentose phosphate and glycolysis of bacteria of step c); ande) selecting a bacterium of step d) which has a metabolic pathway ratio Pentose phosphate/glycolysis superior to 0.5.30. The bacterium of claim 26 , wherein said bacterium can utilize lignin claim 26 , cellulose claim 26 , hemi-cellulose claim 26 , starch claim 26 , xylan claim 26 , polysaccharide claim 26 , xylose claim 26 , galactose claim 26 , sucrose claim 26 , lactose claim 26 , glycerol claim 26 , molasses or glucose claim 26 , or derivatives thereof claim 26 , as a carbon source.31. The bacterium of claim 26 , said bacterium further comprising at least one recombinant nucleic acid sequence encoding an NADPH-dependent enzyme.32. The recombinant bacterium of claim 31 , wherein said at least one recombinant nucleic acid sequence encodes an NADPH-dependent Alcohol dehydrogenase and/or an NADPH-dependent ...

Method for Processing Oil Crops with Rhodotorula

The disclosure discloses a method for processing oil crops with and belongs to the technical field of fermentation. The method includes the step of inoculating the (such as and ) that can produce carotenoid into a fermentation medium that contains oil-rich oil crops for solid state fermentation to obtain oil and oil crop meal rich in carotenoid. The carotenoid as a fermentative metabolite of the has bioactivities of resisting oxidation, preventing vascular sclerosis, enhancing immunity and preventing cancers. Contents of carotenoid in the oil and oil crop meal acquired by the method can be up to 9.071 μg/g and 8.062 μg/g correspondingly. By the method, the oil and oil crop meal rich in carotenoid can be acquired at the same time by just once fermentation and once oil pressing without additional functional substances, and thus the production cost of the functional oil and the fermentation oil crop meal is greatly reduced. 1Rhodotorula. A method for processing oil crops , comprising the steps of performing solid state fermentation on that produces carotenoid in a fermentation medium and obtaining a fermented product in solid state; and pressing oil from the fermented product to obtain oil and oil crop meal; wherein the fermentation medium comprises oil crop powder;wherein the oil crop powder comprises one or more of soybean extruded powder, soybean powder, peanut powder, rapeseed powder, castor powder and sesame powder.2Rhodotorula. The method of claim 1 , wherein performing solid state fermentation comprises inoculating the into the fermentation medium; wherein the fermentation medium is a mixture of oil crop powder and water.3. The method of claim 2 , wherein the fermentation medium comprises claim 2 , by mass claim 2 , the oil crop powder which is 35-45% of a total mass of the fermentation medium and the water which is 55-65% of the total mass of the fermentation medium.4. The method of claim 3 , wherein the fermentation medium consists of the oil crop powder which ...

PRODUCTION OF RETINOL

The present invention is related to a novel enzymatic process for production of vitamin A alcohol (retinol) via conversion of retinal, which process includes the use of heterologous enzymes having activity as retinal reductase, particularly wherein the reaction leads to at least about 90% conversion of retinal into retinol. Said process is particularly useful for biotechnological production of vitamin A. 1. A carotenoid-producing host cell comprising a retinol dehydrogenase [EC 1.1.1.105] , preferably a heterologous retinol dehydrogenase , said host cell producing a retinoid mix comprising retinal and retinol , wherein the percentage of retinol is at least about 90% , preferably 92 , 95 , 97 , 98 , 99 or even 100% compared to the amount of retinal present in said retinoid mix.2. The carotenoid-producing host cell of claim 1 , wherein the retinal to be reduced via action of the retinol dehydrogenase comprises a mix of trans-retinal and cis-retinal claim 1 , wherein the percentage of trans-retinal in said retinal mix is in the range of at least about 61 to 98% claim 1 , preferably at least about 61 to 95% claim 1 , more preferably at least about 61 to 90%.3FusariumFusarium fujikuroi. The carotenoid-producing host cell according to claim 1 , wherein the retinol dehydrogenase is selected from fungi claim 1 , preferably claim 1 , more preferably retinol dehydrogenase is a retinol dehydrogenase (FtRDH).4. The carotenoid-producing host cell according to claim 3 , wherein the FtRDH is selected from a polypeptide with at least about 60% identity to a polypeptide according to SEQ ID NO:1.5Escherichia, Streptomyces, Pantoea, Bacillus, Flavobacterium, Synechococcus, Lactobacillus, Corynebacterium, Micrococcus, Mixococcus, Brevibacterium, Bradyrhizobium, Gordonia, Dietzia, Muricauda, Sphingomonas, Synochocystis, Paracoccus, Saccharomyces, Aspergillus, Pichia, Hansenula, Phycomyces, Mucor, Rhodotorula, Sporobolomyces, Xanthophyllomyces, PhaffiaBlakesleaSaccharomyces, Aspergillus, ...

SOLVENTLESS EXTRACTION PROCESS

The present invention provides a method for extracting lipids from microorganisms without using organic solvent as an extraction solvent. In particular, the present invention provides a method for extracting lipids from microorganisms by lysing cells and removing water soluble compound and/or materials by washing the lysed cell mixtures with aqueous washing solutions until a substantially non-emulsified lipid is obtained. 1. A process for obtaining lipid from microorganisms comprising:(a) growing microorganisms in a culture medium;(b) treating said culture medium and cells of the microorganism to release intracellular lipids by lysing or rupturing the cells, wherein the lysing or rupturing the cells comprises heating the cells to at least 50° C.;(c) subjecting the culture medium containing the released intracellular lipids to gravity separation to form a light lipid-containing phase and a heavy phase;(d) separating said heavy phase from said light phase; and(e) obtaining said lipid from said light phase.2. (canceled)3. The process of claim 1 , wherein said light phase comprises an emulsified lipid.4. The process of further comprising:(f) adding an aqueous extraction solution to said light phase of step (d); and(g) repeating said steps (d) and (f) until said lipid becomes substantially non-emulsified prior to step (e).5. The process of claim 3 , wherein said emulsified lipid comprises a suspension of said lipid in an aqueous solution.6. The process of claim 1 , wherein said heavy phase comprises an aqueous solution.7. The process of claim 1 , wherein said microorganisms are obtained from a fermentation process.8. The process of further comprising adding a base to a fermentation broth.9. The process of claim 8 , wherein said base is selected from the group consisting of hydroxides claim 8 , carbonates claim 8 , bicarbonates claim 8 , phosphates claim 8 , and mixtures thereof.10. The process of further comprising solubilizing at least part of proteinaceous compounds in ...

METHODS OF PRODUCING ASTAXANTHIN OR PRECURSORS THEREOF

The present invention relates to a recombinant bacterium comprising a heterologous biosynthetic pathway converting lycopene to astaxanthin or a precursor thereof, and its use for producing astaxanthin or a precursor thereof. Shown is the production of carotene, zeaxanthin, canthaxanthin and astaxanthin using recombinant expressing genes encoding for lycopene cylase (EC 5.5.1.19), beta-carotene hydroxylase (EC1.14.13.129) and beta-carotene ketolase, namely carotenoid-4,4-beta-ionone ring oxygenase (EC 1.14.11.B16). 133-. (canceled)34Deinococcus. A recombinant bacterium comprising a heterologous nucleic acid sequence encoding a polypeptide exhibiting lycopene cyclase activity and being able to produce beta-carotene when cultured at a temperature greater than 40° C.35. The recombinant bacterium of claim 34 , further comprising a heterologous nucleic acid sequence encoding a polypeptide exhibiting beta-carotene hydroxylase activity.36. The recombinant bacterium of claim 34 , further comprising a nucleic acid sequence encoding a polypeptide exhibiting beta-carotene ketolase activity.37. The recombinant bacterium of claim 34 , wherein the polypeptide exhibiting lycopene cyclase activity is selected from the group consisting of SEQ ID NOs: 1 claim 34 , 3 claim 34 , 5 claim 34 , 7 claim 34 , 9 claim 34 , 75 claim 34 , 77 claim 34 , 79 claim 34 , 81 claim 34 , 83 claim 34 , 85 and 87 claim 34 , and polypeptides exhibiting lycopene cyclase activity and having at least 80% identity to SEQ ID NO: 1 claim 34 , 3 claim 34 , 5 claim 34 , 7 claim 34 , 9 claim 34 , 75 claim 34 , 77 claim 34 , 79 claim 34 , 81 claim 34 , 83 claim 34 , 85 or 87.38. The recombinant bacterium of claim 35 , wherein the polypeptide exhibiting beta-carotene hydroxylase activity is selected from the group consisting of SEQ ID NOs: 11 claim 35 , 13 claim 35 , 15 claim 35 , 17 claim 35 , 19 claim 35 , 21 claim 35 , 23 claim 35 , 25 claim 35 , 89 claim 35 , 91 and 93 and polypeptides exhibiting beta-carotene ...

METHODS OF PRODUCTION OF PRODUCTS OF METABOLIC PATHWAYS

A plurality of isolated polynucleotide sequences encoding enzymes of the astaxanthin pathway is disclosed. The polynucleotides include:

PRODUCTION OF DOCOSAHEXAENOIC ACID, EICOSAPENTAENOIC ACID AND/OR CAROTENOIDS IN MIXOTROPHIC MODE BY NITZSCHIA

New strains of microalgae belonging to the genus allow high-yield production of lipids such as docosahexaenoic acid (DHA) and eicosapentaenoic acid (EPA), and/or carotenoids such as fucoxanthin. Biomass containing the microalgae can be charged with fucoxanthin representing more than 0.2% by weight of the dry matter. 1NitzschiaNitzschia. A biomass consisting essentially of microalgae of the genus and water , wherein said microalgae of the genus are charged with fucoxanthin , said fucoxanthin representing more than 0.2% by weight of the biomass dry matter.2. The biomass of claim 1 , wherein said fucoxanthin represents more than 0.25% by weight of the biomass dry matter.3. The biomass of claim 1 , wherein the biomass comprises at least 30 g/L of dry matter.4. The biomass of claim 1 , wherein the biomass is product of fermentation.5. The biomass of claim 4 , wherein the biomass is obtained from a culture that has been cultured in mixotrophic mode over several generations in a culture medium comprising a carbon-containing substrate.6Nitzschia. The biomass of claim 1 , wherein said microalgae of the genus is the strain FCC 810 claim 1 , deposited with the CCAP (Culture Collection of Algae and Protozoa) under the accession number CCAP 1052/21.7NitzschiaNitzschia brevirostris.. The biomass of claim 1 , wherein said microalgae of the genus capable of being cultured under mixotropic conditions in a culture medium comprising a carbon-containing substrate is the species8. The biomass of claim 1 , wherein said carbon-containing substrate is selected from the group consisting of glucose claim 1 , derivatives of cellulose claim 1 , lactate claim 1 , lactose claim 1 , saccharose claim 1 , acetate claim 1 , glycerol and mixtures thereof.9. The biomass of claim 5 , wherein said carbon-containing substrate in the culture medium is selected from the group consisting of glucose claim 5 , derivatives of cellulose claim 5 , lactate claim 5 , lactose claim 5 , saccharose claim 5 , acetate ...

METHOD FOR PREPARING MICROBIAL PREPARATION AND MICROBIAL PREPARATION PRODUCED BY THE SAME

The present disclosure relates to a method for preparing an aglycone or hydrolyzed glycoside converted from a glycoside and, specifically, to a method for preparing an aglycone or hydrolyzed glycoside from a glycoside by converting a glycoside into an aglycone form or hydrolyzed glycoside by using a microorganism producing β-glycosidase, and then recovering the aglycone or hydrolyzed glycoside accumulated in the cells of the microorganism. 1. A microorganism or a lysate thereof , wherein the microorganism is a β-glycosidase-expressing microorganism and contains intracellularly accumulated converted ginsenoside or hydrolyzed glycoside.2. The microorganism or lysate of claim 1 , wherein said intracellularly accumulated converted ginsenoside or hydrolyzed glycoside comprises one selected from the group consisting of rg3 claim 1 , rc and f2.3ginseng. The microorganism or lysate of claim 1 , wherein said intracellularly accumulated converted ginsenoside or hydrolyzed glycoside comprises one extracts comprise one or more of a ginsenoside selected from the group consisting of rg1 and rb1.4. The microorganism or lysate of claim 1 , wherein said intracellularly accumulated converted ginsenoside or hydrolyzed glycoside comprises one or more selected from the group consisting of genistein claim 1 , daidzein claim 1 , glycitein claim 1 , sapogenin claim 1 , 3 claim 1 ,4-Hydroxyphenylactic acid claim 1 , 4-HPA claim 1 , m-coumaric acid claim 1 , p-coumaric acid claim 1 , O-beta-D-glucuroniodes claim 1 , stilbenoids claim 1 , rutin claim 1 , quercetin claim 1 , hesparetin claim 1 , baicalein claim 1 , wogonin claim 1 , mogroside IIIE claim 1 , mendelonitrile claim 1 , benzaldehyde claim 1 , and coumarin-derived compounds.5. The microorganism or lysate of claim 1 , wherein said intracellularly accumulated converted ginsenoside or hydrolyzed glycoside comprises one or more of rg3 claim 1 , rc claim 1 , and f2.6bifiduscorynebacterium, aspergillusclostridium.. The microorganism or ...

CAROTENOIDS FOR TREATING OR PREVENTING NAUSEA

Methods and compositions comprising carotenoids for the treatment or prevention of nausea, e.g., chemotherapy-induced nausea and vomiting. 1. A method for the treatment of nausea and/or vomiting in a subject , the method comprising: administering to a subject in need thereof a therapeutically effective amount of a C50 carotenoid compound.2. The method of claim 1 , wherein the subject has or is at risk of developing nausea and/or vomiting associated with chemotherapy or radiation.3. The method of claim 2 , wherein the subject has or is at risk of developing chemotherapy-induced nausea and vomiting (CINV) or radiation-induced nausea and vomiting (RINV).4. The method of claim 1 , wherein the subject has or is at risk of developing post-operative nausea and vomiting (PONV).5. The method of claim 1 , wherein the C50 carotenoid compound is selected from the group consisting of decaprenoxanthin claim 1 , C50-astaxanthin claim 1 , C50-β-carotene claim 1 , C50-carotene (n=3) (16 claim 1 ,16-diisopentenylphytoene) claim 1 , C50-zeaxanthin claim 1 , C50-caloxanthin claim 1 , C50-nostoxanthin sarcinaxanthin claim 1 , sarprenoxanthin claim 1 , acyclic C50 carotenoid bacterioruberin claim 1 , C50-canthaxanthin claim 1 , C50-lycopene claim 1 , C50-phytoene claim 1 , and combinations thereof.6. The method of claim 5 , wherein the C50 carotenoid compound is decaprenoxanthin.7. The method of claim 1 , wherein the step of administering comprises administering a composition that is or comprises: (i) a C50-carotenoid-compound-synthesizing microbe or component thereof claim 1 , (ii) a C50-carotenoid-compound-synthesizing microbe extract claim 1 , (iii) an extracted carotenoid compound claim 1 , or (iv) a combination thereof.8. The method of claim 7 , wherein the C50-carotenoid-compound-synthesizing microbe is viable or alive.9. The method of claim 8 , wherein the step of administering comprises administering a sufficient amount of the microbe to colonize the subject's microbiome.10. The ...

METHOD OF CULTURING HAEMATOCOCCUS SPECIES FOR MANUFACTURING OF ASTAXANTHIN

A method of culturing species for manufacturing of astaxanthin comprising the steps of: providing a substrate, arranging the species on the surface of the substrate, exposing the species arranged on the substrate to high light intensities from the beginning of a culturing process and avoiding a two-step culturing process of the species with a first step which is an initial culturing taking place by exposure of the species to low light energy followed by a second step of subsequent culturing of the species by exposure of the species to higher light energy than applied in the first step to induce astaxanthin formation, and optionally—harvesting the cultured species and/or—isolating astaxanthin. 19-. (canceled)10Haematococcus. A process for an improved astaxanthin production comprising a biofilm cultivation of on a substrate in a one-step procedure at high light intensity to increase biomass productivity and induce and increase astaxanthin production at the same time , wherein high light intensity is 200 μmol photons msor more.11Haematococcus. The method of claim 10 , wherein the astaxanthin is isolated from harvested species.12HaematococcusHaematococcus pluvialis.. The method of claim 10 , wherein the species is13. The method of claim 10 , wherein the substrate is a sheet-like material.14. The method of claim 13 , wherein the substrate is a porous sheet.15. The method of claim 13 , wherein the sheet-like material is selected from the group consisting of paper claim 13 , cellulose ester claim 13 , in particular cellulose acetate claim 13 , mixed cellulose ester claim 13 , cellulose claim 13 , cellulose nitrate claim 13 , polyamides claim 13 , polyesters and polyolefins.16. The method of claim 10 , wherein a light/dark cycle of 14/10 hours is used in combination with 5% COduring the cultivation.17. The method of claim 10 , wherein the amount of astaxanthin is higher than 4 g m.18. The method of claim 10 , wherein a low-light exposure is not mandatory claim 10 , wherein ...

Methods for treating a culture of haematococcus pluvialis for contamination using salt and hydrogen peroxide

Methods of treating contamination, particularly fungal contamination, in cultures of Haematococcus pluvialis with hydrogen peroxide and salt are described herein. The method comprises dosing the culture comprising a concentration of salt with a concentration of hydrogen peroxide based on the stage of the cells in the culturing process and at a frequency to increase the likelihood of the cells surviving until the process of accumulating carotenoids, such as astaxanthin, is complete.

BIOLOGICAL DEVICES AND METHODS OF USE THEREOF TO PRODUCE CAROTENOIDS

Described herein are devices and methods for using the same to produce carotenoids. The carotenoids produced by the devices and methods disclosed herein do not require the ultra purification that is common in conventional or commercial methods. The devices and methods disclosed herein also enhance one or more physical properties of plants treated with the devices described herein. 1. A DNA construct comprising the following genetic components: (a) a gene that expresses beta-carotene hydroxylase , (b) a gene that expresses lycopene epsilon cyclase , and (c) a gene that expresses 1-deoxy--xylylose phosphate synthase.2. The DNA construct of claim 1 , further comprising at least one promoter claim 1 , wherein the promoter is positioned before the gene that expresses beta-carotene hydroxylase claim 1 , the gene that expresses lycopene epsilon cyclase claim 1 , the gene that expresses 1-deoxy--xylylose phosphate synthase claim 1 , or any combination thereof.3. The DNA construct of claim 1 , further comprising at least one promoter positioned before the gene that expresses beta-carotene hydroxylase claim 1 , the gene that expresses lycopene epsilon cyclase claim 1 , and the gene that expresses 1-deoxy--xylylose phosphate synthase claim 1 , or any combination thereof.4. The DNA construct of claim 1 , wherein the promoter is a GAL1 promoter.5. The DNA construct of claim 1 , wherein the gene that expresses 1-deoxy--xylulose phosphate synthase is SEQ ID NO. 1 or a sequence having at least 90% homology thereof.6. The DNA construct of claim 1 , wherein the gene that expresses beta-carotene hydroxylase is SEQ ID NO. 2 or a sequence having at least 90% homology thereof.7. The DNA construct of claim 1 , wherein the gene that expresses lycopene epsilon-cyclase is SEQ ID NO. 3 or a sequence having at least 90% homology thereof.8. The DNA construct of claim 1 , further comprising at least one terminator.9. The DNA construct of claim 8 , wherein the terminator is a CYC1 terminator.10. ...

Microbial engineering for the production of chemical and pharmaceutical products from the isoprenoid pathway

The invention relates to the production of one or more terpenoids through microbial engineering, and relates to the manufacture of products comprising terpenoids.

PRODUCTION OF TRANS-RETINAL

The present invention is related to a novel enzymatic process for production of vitamin A aldehyde (retinal) via stereoselective conversion of beta-carotene which process includes the use of trans-selective enzymes having activity as beta-carotene oxidases (BCOs), in particular having preference for trans-retinal. 5 Said process is in particular useful for biotechnological production of vitamin A. 1. A carotenoid-producing host cell comprising a stereoselective beta-carotene oxidizing enzyme (BCO) , said host cell producing a retinal mix comprising cis- and trans-retinal , wherein the percentage of trans-retinal in the mix is at least about 65% , preferably 68 , 70 , 75 , 80 , 85 , 90 , 95 , 98% or up to 100% produced by said host cell.2. The carotenoid-producing host cell of claim 1 , wherein the percentage of trans-retinal in the retinal mix comprising trans- and cis-retinal is in the range of about at least 65 to 98% claim 1 , preferably about at least 65 to 95% claim 1 , more preferably at least about 65 to 90% based on the total amount of retinal produced by said host cell.3. The carotenoid-producing host cell according to comprising a heterologous stereoselective BCO.4Fusarium, Ustilago, Crocus, Drosophila, Danio, Ictalurus, Esox, LatimeriaFusarium fujikuroi, Ustilago maydis, Crocus sativus, Drosophila melanogaster, Danio rerio, Ictalurus punctatus, Esox lucius, Latimeria chalumnae.. The carotenoid-producing host cell according to claim 1 , wherein the BCO is selected from fungi claim 1 , plants or animal claim 1 , preferably selected from claim 1 , more preferably selected from5. The carotenoid-producing host cell according to claim 4 , wherein the BCO is selected from a polypeptide with at least about 60% identity to a polypeptide according to sequences known from the database such as EAK81726 claim 4 , AJ854252 claim 4 , Q84K96.1 claim 4 , or with at least 50% identity to a polypeptide according to sequence known from the database as Q90WH4.6. The ...

Solventless Extraction Process

The present invention provides a method for extracting lipids from microorganisms without using organic solvent as an extraction solvent. In particular, the present invention provides a method for extracting lipids from microorganisms by lysing cells and removing water soluble compound and/or materials by washing the lysed cell mixtures with aqueous washing solutions until a substantially non-emulsified lipid is obtained. 1. A process for obtaining lipid from microorganisms comprising:(a) lysing cells of the microorganisms to produce a lysed cell mixture;(b) treating said lysed cell mixture to produce a phase separated mixture comprising a heavy layer and a light layer, wherein said heavy layer comprises an aqueous solution and said light layer comprises said lipid;(c) separating said heavy layer from said light layer; and(d) obtaining said lipid from said light layer.2. The process of claim 1 , wherein said step (b) comprises centrifuging said lysed cell mixture.3. The process of claim 2 , wherein said light layer comprises an emulsified lipid.4. The process of further comprising:(e) adding an aqueous extraction solution to said light layer of step (c); and(f) repeating said steps (b), (c) and (e) until said lipid becomes substantially non-emulsified prior to said step (d).5. The process of claim 3 , wherein said emulsified lipid comprises a suspension of said lipid in an aqueous solution.6. The process of claim 1 , wherein said aqueous solution comprises solid cell materials.7. The process of claim 1 , wherein said microorganisms are obtained from a fermentation process.8. The process of further comprising adding a base to a fermentation broth.9. The process of claim 8 , wherein said base is selected from the group consisting of hydroxides claim 8 , carbonates claim 8 , bicarbonates claim 8 , and mixtures thereof.10. The process of further comprising solubilizing at least part of proteinaceous compounds in a fermentation broth.11. The process of claim 1 , wherein ...

MICROBIAL ENGINEERING FOR THE PRODUCTION OF CHEMICAL AND PHARMACEUTICAL PRODUCTS FROM THE ISOPRENOID PATHWAY

The invention relates to the production of one or more terpenoids through microbial engineering, and relates to the manufacture of products comprising terpenoids. 1207. -. (canceled)208. A method for making a pharmaceutical product , the method comprising:{'i': Escherichia coli', 'E. coli, 'providing an () that produces isopentenyl pyrophosphate (IPP) and dimethylallyl pyrophosphate (DMAPP) through an upstream methylerythritol pathway (MEP) and converts the IPP and DMAPP to amorphadiene or derivative thereof through a recombinantly expressed downstream synthesis pathway comprising farnesyl diphosphate synthase and amorphadiene synthase;'}{'i': 'E. coli', 'culturing the to produce the amorphadiene or derivative thereof, wherein the accumulation of indole in the culture is controlled to below 100 mg/L to thereby increase production of amorphadiene or derivative thereof; and'}incorporating the amorphadiene or derivative thereof, into a pharmaceutical product.209. The method of claim 208 , wherein accumulation of indole in the culture is controlled by balancing the upstream MEP pathway with the downstream terpenoid synthesis pathway.210. The method of claim 208 , further comprising measuring the amount or concentration of indole continuously or intermittently.211. The method of claim 208 , wherein accumulation of indole in the culture is maintained to below 50 mg/L.212. The method of claim 208 , wherein accumulation of indole in the culture is maintained to below 10 mg/L.213. The method of claim 208 , wherein the amorphadiene or derivative thereof is produced at 10 mg/L or more.214. The method of claim 208 , wherein the amorphadiene or derivative thereof is produced at 100 mg/L or more.215E. coli. The method of claim 208 , wherein the has additional copies of one or more of the dxs claim 208 , idi claim 208 , ispD claim 208 , and ispF genes of the MEP pathway.216E. coli. The method of claim 215 , wherein the has a heterologous dxs-idi-ispDF operon.217. The method of ...