Chromatographic process

... 1,135,522. Liquid chromatography. E. V. PIEL. 30 Aug., 1966 [15 Sept., 1965; 22 Dec., 1965], No. 38557/66. Heading B1X. In liquid column chromatography the chromatography bed consists of particles which have an average size of less than 1.5 microns, preferably less than 1 micron. The liquid mixture to be separated is caused to flow rapidly, preferably by centrifugal force, through the column and is separated by partition between the bed and a moving liquid solvent which is passed through the bed. In operating the centrifuge bucket apparatus of Fig. 1 a bed of particles is established by adding a slurry of particles and a solvent into tube 12 via reservoir 19, the bed being compacted by centrifuging the tube. A sample to be resolved is added to the top surface of the bed and the tube centrifuged thereby establishing chromatographic bands in bed 13 which can be identified visually or 'by elution. With the continuously operating apparatus of Figure 3 the particles e.g. a gel or thixotropic ...

Integrated protein affinity capture centrifugation device

PROCESS FOR ANALYTICAL DETERMINATIONS AND ROTOR INSERT ELEMENT THEREFOR

ABSTRACT The present invention provides a process for carrying out analytical determinations by mixing and incubating a sample solution with at least one reagent solution and optical measurement of one parameter in the incubated reaction solution mixture, mixing, incubating and measuring being carried out during the action of a centrifugal force, wherein, under the influence of the centrifugal force, the sample solution is brought together with a soluble dry reagent, with at least partial dissolving thereof, and the mixture or the solution is forced centrifugally through a plurality of small hollow spaces, the centrifugal force and the flow resistance of the small hollow spaces being so adapted with regard to one another that a complete dissolving and mixing of the reaction components and possibly incubation takes place before the reaction solution passes from the small hollow spaces into a measuring chamber in which the measure-ment is carried out. The present invention also provides an insert element for a centrifugal analysis rotor, wherein it comprises at least one analysis reagent in dry form and has a plurality of very small interconnecting hollow spaces which connect a sample providing chamber with a measuring chamber.

PROCESS FOR ANALYTICAL DETERMINATIONS AND ROTOR INSERT ELEMENT THEREFOR

Procédé de séparation des composants d'un échantillon liquide

Appareil pour réaliser une interaction physique et chimique de substances fluides au voisinage immédiat de surfaces solides

CENTRIFUGAL CHROMATOGRAPHY MECHANISM.

Reagent for detecting antigen-antibody reactions

A solvent-based mixt. for chromatographic analyses is applied to the centre of a circular disc (4) of adsorbent material resting on a support plate (15) in a rotor (3) driven by a central shaft (10) within an enclosed chamber. Centrifugal material is intercepted by the inner wall of a collector ring (5) round the support plate and rotated by the same shaft. The inner wall of the collector ring is inclined, and each point on its inner wall is farther from the rotation axis (14) than the most distant point of the adsorbent disc. At two or more (even numbered) points in the slightly irregular collector ring, at max. radial distance from the centre, are discharge bores (6) taking solvent towards an axial outlet, e.g. for re-use.

PLANAR CENTRIFUGAL CHROMATOGRAPHY MECHANISM.

Reagent for detecting antigen-antibody reactions

A solvent-based mixt. for chromatographic analyses is applied to the centre of a circular disc (4) of adsorbent material resting on a support plate (15) in a rotor (3) driven by a central shaft (10) within an enclosed chamber. Centrifugal material is intercepted by the inner wall of a collector ring (5) round the support plate and rotated by the same shaft. The inner wall of the collector ring is inclined, and each point on its inner wall is farther from the rotation axis (14) than the most distant point of the adsorbent disc. At two or more (even numbered) points in the slightly irregular collector ring, at max. radial distance from the centre, are discharge bores (6) taking solvent towards an axial outlet, e.g. for re-use.

IONIC LIQUID SEPARATIONS

A process for the separation of inorganic compounds comprises centrifugal partitioning of at least one inorganic compound between a mobile liquid phase and an immiscible stationary liquid phase. At least one of the mobile liquid phase and the stationary liquid phase comprises or consists of an ionic liquid. A rotary coil centrifuge for counter current chromatography and a liquid-liquid chromatography or liquid-liquid extraction apparatus may be used in the process.

Vacuum assisted affinity chromatography device and method

Sample preparation device and method particularly useful for large volume capture and small volume elution. The device comprising a manifold, a sample holder or reservoir, and a filter unit containing chromatography media such that a filtration path is established between the sample holder, the filter unit and the manifold. Upon subjecting the sample in the sample holder to a driving force such as vacuum, the sample flows through the chromatography media in the filter unit. Molecules of interest bind to the media and any unwanted molecules can be washed under vacuum mode. Elution can then be carried out by removing the filter unit and subjecting the media to a driving force such as vacuum or centrifugation. In another embodiment, molecules of interest can be eluted into a low volume centrifugal spinner directly in the manifold.

VERFAHREN ZUR DURCHFUEHRUNG ANALYTISCHER BESTIMMUNGEN UND HIERFUER GEEIGNETES ROTOREINSATZELEMENT

Apparatus for Effecting Separations of Fluids at Extended Solid Surfaces by means of Chromatography

... 1,175,736. Chromatographic apparatus. RESEARCH CORP. 19 April, 1967 [18 May, 1966], No. 17892/67. Headings B1L and B1X. [Also in Division G4] Fluids are subjected to chromatographic separation by passage between a pair of parallel planar extended surfaces separated by 0.00004-0.25 in. (see Fig. 1, not shown). The fluids may be either gases or liquids, and the space between the surfaces may be filled with a suitable chromatographic adsorbent. The adsorbent or surfaces may be coated with a stationary liquid phase such as dioctyl phthalate, and the surfaces preferably define a plurality of parallel channels. If desired, several surfaces may be superposed to provide several spaces in parallel (see Fig. 6, not shown) or one elongated space (see Fig. 8, not shown). In a preferred embodiment (see Figs. 3 and 4), the surfaces are circular plates 141, 161 which are rotated, and the liquid or gas mixture to be analysed is fed by line 50 to a point in the space between the plates ...

PROOF OF ABNORMEN REACTIONS IN AN ERYTHROCYTE AGGLUTINATION

DEVICE AND PROCEDURE FOR THE TREATMENT OF A FLUID DURING IT A CENTRIFUGAL ENERGY ARE SUBJECTED.

MICROCHROMATOGRAPHIC DEVICE AND METHOD FOR RAPID DETERMINATION OF A DESIRED SUBSTANCE

ABSTRACT OF THE DISCLOSURE A combination of a microcolumn packed with a suitable absorbent material and a centrifuge tube, is disclosed. A predetermined volume of an eluent being capable of selectively eluting a desired substance through the microcolumn is contained above the packing of the absorbent material. The microcolumn and the centrifuge tube are dimensioned in such a manner that upon centrifugation of the predetermined volume of the eluent through the microcolumn, a level of the eluent remaining in the microcolumn, and a level of the eluent in the centrifuge tube coincide substantially at or above a top surface of the packing of the absorbent material. Consequently, centrifugation of the assembly of the microcolumn and the centrifuge tube causes passage of the predetermined volume of eluent through the microcolumn. Determination or assay of the selectively eluted desired substance may be performed by spectrophotometric methods. The disclosed device and method is particularly adapted for the determination or assay of a relative concentration of glyco-sylated hemoglobin species present in the red blood cells of a specific person.

TYPE-XLL CROSS-AXIS SYNCHRONOUS FLOW-THROUGH COIL PLANET CENTRIFUGE FOR SEPARATION OF BIOPOLYMERS

A cross-axis synchronous, flow-through coil planet centrifuge having exclusively left-handed column coils (12) and the method for separating macromolecules utilizing the left-handed column coil centrifuge. The use of left-handed column coils (12) exclusively allows retention of the stationary phase when utilizing aqueous-aqueous, two-phase solvent systems. The cross-axis synchronous flow-through coil planet centrifuge having left-handed column coils (12) has been utilized to separate proteins utilizing an aqueous-aqueous polymer phase solvent system.

ASSEMBLY HAS COLUMN SEPARATION OR REACTION HOUSABLE IN CENTRIFUGE

METHOD AND DEVICE FOR MICRO-FLUIDIC ANALYSIS, IN PARTICULAR URANIUM AND PLUTONIUM IN RADIOACTIVE SAMPLES

IMPROVEMENTS RELATING TO THE PREPARATIVE SEPARATION OF FLUID SAMPLES

... 1282424 Liquid chromatography H REEVE ANGEL & CO Ltd 29 Sept 1969 [25 Feb 1969] 10086/69 Heading B1X Continuous liquid chromatographic separation is achieved by passing the sample to be separated in a solvent continuously by line 57 to the inner periphery of annular separation medium 1 held between glass or plastics plates 21, 22 and causing the annular medium to rotate, e.g. by means 35, relatively to both the sample input line and collection means 39, 40. Initially all the sample is adsorbed, but different eluents supplied by lines 58 and 59 and suitable adjustment of the speed of rotation cause the components to separate into spiral swathes 44, 46 &c so that they may be collected separately at appropriate points on the outer periphery of annulus 1. In an example, insulin is separated in this manner from proteins using carboxymethylcellulose as absorbent, HC1 of pH3 being employed first to elute the proteins and then HC1 of pH2 to desorb the insulin.

PROCEDURE FOR THE EXECUTION OF ANALYTIC REGULATIONS AND FOR THIS APTITUDE ROTOR EMPLOYMENT ELEMENT.

METHODS AND APPARATUS FOR CENTRIFUGAL LIQUID CHROMATOGRAPHY

Apparatus and methods related to centrifugal liquid chromatography are described. An angular velocity can be simultaneously imparted to a large number of chromatographic enclosures. Via centrifugal forces, a mobile phase fluid including a sample can be driven through a stationary phase within the chromatographic enclosure to perform a chromatographic separation process on components of the sample. The use of centrifugation as a driving force can allow significantly smaller stationary phase particles to be employed as compared to high performance liquid chromatography (HPLC). Further, for an equivalent chromatographic separation process, the use of centrifugation can provide much greater separation efficiencies than HPLC.

Centrifugal liquid-liquid partition chromatography device for separating e.g. chiral molecule in cosmetic industry, has chromatographic columns subjected to centrifugation, and continuous extraction unit to continuously extract solutes

Procédé et dispositif de chromatographie comprenant au moins une colonne soumise à une centrifugation ou au moins un ensemble de dispositifs de centrifugation à cellules (1, 2), au moins un système de solvants biphasique (6, 7), et un moyen d'injection (4) d'une solution (5) comprenant des solutés à extraire, caractérisé en ce qu'il comprend des moyens d'extraction en continu desdits solutés. Lesdits moyens d'extraction en continu sont formés d'au moins deux phases liquides non miscibles (6, 7) à contre-courant vrai continu dudit au moins un système de solvants biphasique et de cellules comportant chacune deux entrées (14, 16) et deux sorties (15, 17) correspondant à la phase lourde (7) et légère (6) dudit au moins un système de solvants biphasique.

Non-miscible liquid-liquid separating method for disk/monoblock type cylindrical rotor, involves adjusting rotor rotation direction and driving direction to oppositely set driving direction and circuit's content driving direction

Le système de séparation liquide-liquide CPC comprend un rotor cylindrique (1) monobloc, entraîné en rotation autour de son axe (14) par un moteur (16) électrique, avantageusement triphasique. Le corps cylindrique comporte des cellules (2) disposées sur plusieurs niveaux et reliées en série.Des moyens de pompage permettent de contrôler la circulation de la phase mobile. Le système comporte un dispositif d'ajustement relatif entre le sens de rotation du rotor et le sens de circulation de la phase mobile pour mettre en opposition ledit sens de circulation et un sens d'entraînement par effet de vis d'Archimède du contenu du circuit vers une des extrémités du circuit. Pour éviter que le débit de la vis d'Archimède soit colinéaire avec la circulation de la phase mobile et empêcher la formation de cavitation, on réalise une inversion du sens de rotation.

MICROCHROMATOGRAPHIC DEVICE AND METHOD FOR RAPID DETERMINATION OF A DESIRED SUBSTANCE

FLUID TREATING PROCESS AND APPARATUS

KEYED COLUMN CHROMATOGRAPHY APPARATUS

MICROCHROMATOGRAPHIC DEVICE AND METHOD FOR RAPID DETERMINATION OF A DESIRED SUBSTANCE

DEVICE AND PROCESS OF ESTABLISHMENT OF CONTACT BETWEEN IMMISCIBLE LIQUID PHASES BY THE CENTRIFUGAL FORCE

La présente invention concerne un dispositif et un procédé de mise en contact de phases fluides immiscibles par la force centrifuge, en particulier pour réaliser l'extraction de composés de l'une de ces phases par une autre phase. Ce dispositif comporte au moins une unité de mise en contact (3') qui est apte à être entraînée en rotation autour d'un axe (X'X) et qui comprend une pluralité de cellules (210, A, D) destinées à être parcourues successivement par ces phases dans un sens de circulation donné, chaque cellule étant pourvue de deux canaux d'entrée/ sortie (214 et 215) qui relient de manière fluidique les cellules deux à deux entre elles et qui débouchent dans chaque cellule respectivement par deux orifices d'entrée/ sortie respectivement proximal (214a) et distal (215a) par rapport audit axe. Selon l'invention, pour au moins deux cellules adjacentes (A et D) de ladite au moins une unité, leurs deux orifices proximaux (214a) ou bien distaux (215a) sont reliés directement entre eux par l'un de ces canaux (214 ou 215), de telle sorte que ces phases circulent à travers l'une de ces cellules adjacentes vers ledit axe et à travers l'autre cellule en s'éloignant dudit axe. The present invention relates to a device and a method for contacting immiscible fluid phases by centrifugal force, in particular to carry out the extraction of compounds from one of these phases by another phase. This device comprises at least one contacting unit (3 ') which is adapted to be rotated about an axis (X'X) and which comprises a plurality of cells (210, A, D) intended to be successively traversed by these phases in a given direction of circulation, each cell being provided with two input / output channels (214 and 215) which fluidly connect the cells two by two to each other and which open into each cell respectively by two inlet / outlet ports respectively proximal (214a) and distal (215a) with respect to said axis. According to ...

DEVICE AND PROCESS OF CHROMATOGRAPHY HAVE AGAINST TRUE CURRENT

Apparatus for purifying nucleic acid and method of purifying nucleic acid

A nucleic acid refining apparatus, being ease in automation thereof, keeping high in contacting frequency between nucleic acid within a sample and a solid phase during nucleic acid capture processing, thereby proving high capturing rate, comprises: means for separating a liquid containing the nucleic acid therein from said sample through centrifugal force; means for transferring a reagent through the centrifugal force; means for producing a mixture liquid of said reagent transferred through the centrifugal force and a solution containing said nucleic acid therein; a carrier for capturing said nucleic acid; means for transferring said mixture liquid to said carrier through the centrifugal force; heating means for heating said carrier; and a holding means for separating and holding the reagent containing said nucleic acid eluting from said carrier, separating from other reagent, through different centrifugal.

IMMUNOAFFINITY COLUMN FOR SIMULTANEOUS EXTRACTION OF SEVERAL ANABOLIC HORMONE RESIDUES

A column for the simultaneous extraction of several anabolic hormone residues from biological liquids or biological tissue homogenates digested with proteolytic enzymes contains two or more gel fractions having a single polysaccharide matrix but each conjugated with a specific immunoglobulin for one of the hormones to be isolated. Conjugation may be effected by incubating an aqueous solution of said immunoglobulin with an aqueous suspension of a respective gel fraction for a time of between 3 and 12 hours at a temperature of between 4 and 25 DEG C.

Methods and compositions for chromatography

INTEGRATED PROTEIN AFFINITY CAPTURE CENTRIFUGATION DEVICE

DETECTING ABNORMAL REACTIONS IN A RED BLOOD CELL AGGLUTINATION

Abnormal reactions in a red blood cell classification by agglutination, are checked following centrifugation of a sample in a column of a cassette, the column containing microparticles. This is done by imaging the column on a detector array that is used to correlate the images with predefined red cell classes based upon the distribution of the images across the column. However, prior to the correlation step, abnormal reactions are checked for by detecting whether any of the following is present: i) errors that cause imaged features of the column or any pellet produced therein to be out of range; ii) hemolysis of the sample; iii) insufficient or too many blood cells present; iv) mixed field agglutination; and v) presence of fibrin at the top of the microparticles.

METHOD AND APPARATUS FOR ADSORPTION DETECTION

A method and apparatus for adsorption and detection of adsorbents or analytes. The method and apparatus can be employed in the field for rapid adsorption of analytes and is particularly useful for detection of mycotoxins. A sample to be analyzed is prepared in solution and placed in a test tube. A tube-like adsorption column having a seal and a valve member is forcefully fed into the test tube to force solutions through the valve member into the column and through a filter and adsorbent to trap interferences. The semi-purified solution may then be analyzed for the presence of analytes. The column with the purified solution may be further employed with a second smaller adsorption column similarly equipped with a seal and valve member fitting within the first column. In similar fashion the second column may be forced into the first column to expel the solution therein into the second column and through one or more selective adsorbents for different analytes such as one or more mycotoxins. Detection of the adsorbed analyte may be made by shining a fluorescent or "black" light on the adsorbent which fluoresces to indicate presence of the analyte.

DEVICES AND METHODS FOR THE PURIFICATION, ISOLATION, DESALTING OR BUFFER/SOLVENT EXCHANGE OF SUBSTANCES

A spin column device, which contains a rigid porous filter that retains its shape during centrifugation, chromatography methods using the device to isolate a desired substance, e g, a biological molecule, from other substances in a mixture, and kits containing the device with one or more reagents for use in the method.

[UNK]

INTEGRATED PROTEIN AFFINITY CAPTURE CENTRIFUGATION DEVICE

A method and apparatus are described for screening a proteome comprising an array of affinity elements, whereby a proteome is directed through the affinity array and those components that specifically bind to the affinity array are eleuted in a suitable eluent by means of centrifugation. Suitable eluents can include a proteome component, a chemical library component or an affinity element. This method and apparatus can be used for several purposes, including screening for bio-active compounds, determining physiological targets of known drugs, determining specificity of compound interactions with physiological targets, and for quantitative protein analyses.

METHOD AND UNIT FOR SEQUENTIAL CENTRIFUGAL STRATIFICATION CHROMATOGRAPHY

The method and plant disclosed combine the advantages of the preparatory centrifugal stratification chromatography, of the analytical horizontal, anticircular and sequential thin layer chromatography. In this new on-line preparatory method the addition of solvent may be varied in parallel, locally and temporally. It is thus possible to improve sections of the integral chromatogram in their separation by selecting for these partial problems the optimum composition of the solvent. The separations are basically obtained in two different ways: 1. The circular separations are effected by means of the centrifugal force by using a sequential solvent supply (20), the separation taking place from the inside to the outside. The separated substances may be brought back to the inner part of the layer by recycling. 2. The anticircular separations are performed by means of the capillary force by using an annular-shaped solvent supply (19), the substance zones being concentrated and/or developed from the outside to the inside. The possibilities of circular and anticircular applications may be combined as desired so that the separation path may be prolonged practically in an illimited manner and thus the capacity and separation power may be considerably increased.

CENTRIFUGAL LIQUID CHROMATOGRAPH

PURPOSE: To perform the separating operation of the titled chromatograph highly accurate by forming contact portions between a separation portion and an eluate supply portion in plane-like shapes that are in parallel with a rotating surface of the separation portion, and by providing a resilient member for the elute supply portion. CONSTITUTION: An introducing pipe 28 of an eluate supply portion 20 is downward pushed by a coiled spring 27 and contacted withd a separation portion 10 via a seal 23 in an airtight shape. The pipe 28 is supported to a lower portion of a disc portion by a lower plate 24. Revolution-stop shafts 26 are loosely penetrated through the holes of the disc portion. Accordingly, even when the separation portion 10 is inclined while it is rotated, the pipe 28 inclins using a support point of the lower plate 24 as a fulcrum. As a result, no excessively great force is applied to a contact surface between the seal 23 and a cover of a cylinderical body 15. The seal 23 is made ...

IMMUNOAFFINITY COLUMN FOR THE SIMULTANEOUS EXTRACTION OF SEVERAL ANABOLIC HORMONE RESIDUES FROM BIOLOGICAL LIQUIDS AND THE METHOD FOR ITS PREPARATION

MICROCHROMATOGRAPHIC DEVICE

METHOD AND APPARATUS FOR THE ANALYSIS OF AGGLUTINATION REACTIONS

DEVICES AND METHODS FOR THE PURIFICATION, ISOLATION, DESALTING OR BUFFER/SOLVENT EXCHANGE OF SUBSTANCES

A spin column device, which contains a rigid porous filter that retains its shape during centrifugation, chromatography methods using the device to isolate a desired substance, e g, a biological molecule, from other substances in a mixture, and kits containing the device with one or more reagents for use in the method.

TOGETHER HAS TENANTABLE REACTION OR DISTILLATION COLUMN OUT OF CENTRIFUGAL MACHINE

Planar centrifugal chromatography device

A planar centrifugal chromatography device includes a chamber (2), and a rotor (3) arranged therein, driven by a motor (12) and serving as a separating element. The rotor has a supporting plate (15) with an adsorbing medium (4) provided thereon. A collecting device (5) is arranged near the outer edge of the rotor, but within the chamber. The characteristics of the invention include the collecting device (5) and the rotor (3) being mounted on a common shaft (10) and each individual point of a wall of the collecting device (5) being farther away from the axis of rotation (14) than the point of the adsorbing medium (4) which is farthest from the axis of rotation (14), and include the distances of individual points on the wall of the collecting device (5), measured from the axis of rotation (14), varying. At each point on the collecting device which is farther from the axis than two points which are adjacent to it, there is arranged an output opening (6) which is connected by a discharge channel ...

Vorrichtung zum chromatographischen Trennen von Gemischen

counter-current chromatographic arrangement

ANALYTICAL METHOD USING CENTRIFUGAL FORCE

Detecting abnormal reactions in a red blood cell agglutination

ANALYTICAL METHOD USING CENTRIFUGAL FORCE

The present invention provides a process for carrying out analytical determinations by mixing and incubating a sample solution with at least one reagent and optically measuring a parameter in the incubated reaction mixture. The mixing, incubating and measuring are carried out during the action of a centrifugal force which forces the solution through a plurality of interconnected small hollow spaces having flow resistance so adapted with regard to one another as to mix the reaction components. Incubation may also take place before the reaction solution passes from the plurality of interconnected small hollow spaces into a measuring chamber in which the measurement is carried out.

INTEGRATED PROTEIN AFFINITY CAPTURE CENTRIFUGATION DEVICE

A method and apparatus are described for screening a proteome comprising an array of affinity elements, whereby a proteome is directed through the affinity array and those components that specifically bind to the affinity array are eleuted in a suitable eluent by means of centrifugation. Suitable eluents can include a proteome component, a chemical library component or an affinity element. This method and apparatus can be used for several purposes, including screening for bio-active compounds, determining physiological targets of known drugs, determining specificity of compound interactions with physiological targets, and for quantitative protein analyses.

TREATMENT APPARATUS

ABSTRACT Apparatus for treating a fluid material which apparatus comprises (a) a permeable element at least a portion of which is permeable to the fluid material and/or, where the fluid material is treated with a fluid in apparatus according to the invention, to the fluid, which permeable element is rotatable about an axis, (b) means to charge the fluid material to the permeable element and (c) means to discharge the fluid material or a component or derivative thereof from the permeable element characterised in that the apparatus is adapted to discharge the fluid material or a component or derivative thereof from the permeable element . axially distant the position at which the fluid material is charged thereto.

Apparatus for purifying nucleic acid and method of purifying nucleic acid

METHOD AND APPARATUS FOR MICRO-FLUIDIC ANALYSIS, IN PARTICULAR URANIUM AND PLUTONIUM IN RADIOACTIVE SAMPLES

Dispositif d'analyse de radioéléments dans un échantillon radioactif, caractérisé en ce qu'il comprend au moins une cellule d'analyse microfluidique (1) essentiellement plane comprenant au moins : . un réservoir (101) configuré pour accueillir une quantité prédéterminée d'un fluide à analyser contenant une quantité de l'échantillon à analyser, . une colonne de séparation (103) comprenant un monolithe organique, reliée au réservoir (101) adaptée pour la séparation d'un radioélément d'intérêt, . des moyens de déplacement adaptés pour faire déplacer le fluide à travers la colonne de séparation (103), o des moyens pour accueillir le fluide (107) après passage à travers la colonne de séparation (103) situés en aval de la colonne de séparation (103), dans le sens de l'écoulement du fluide à analyser. L'invention concerne également un procédé d'analyse mettant en oeuvre un tel dispositif d'analyse. Device for the analysis of radioelements in a radioactive sample, characterized in that it comprises at least one essentially flat microfluidic analysis cell (1) comprising at least: a reservoir (101) configured to receive a predetermined amount of a fluid to be analyzed containing an amount of the sample to be analyzed, a separation column (103) comprising an organic monolith, connected to the reservoir (101) adapted for the separation of a radioelement of interest, displacement means adapted to move the fluid through the separation column (103); means for receiving the fluid (107) after passing through the separation column (103) located downstream of the separation column ( 103), in the direction of flow of the fluid to be analyzed. The invention also relates to an analysis method implementing such an analysis device.

CENTRIFUGAL CHROMATOGRAPHY DEVICE

Trennvorrichtung zur Behandlung von Biomolekülen

APPARATUS AND PROCESS FOR TREATING A FLUID MATERIAL WHILE IT IS SUBJECTED TO A CENTRIFUGAL FORCE

Ionic liquid separations

DEVICE AND PROCESS OF ESTABLISHMENT OF CONTACT BETWEEN IMMISCIBLE LIQUID PHASES BY THE CENTRIFUGAL FORCE

Ensemble a colonne de separation ou de reaction logeable en centrifugeuse

L'organe recepteur 2 contient un materiau de colonne 4, destine a etre traverse par un materiau echantillon. L'organe recepteur 2 s'emboite dans un recipient de centrifugation 14 jusqu'a ce qu'une butee 16 de l'organe recepteur 2 s'appuie sur un bord 18 du recipient de centrifugation 14, dont l'extremite inferieure peut recevoir le materiau echantillon apres qu'il a traverse le materiau de colonne 4 sous l'effet de la centrifugation dans une centrifugeuse. Utilisation pour supprimer la necessite d'utiliser une solution-tampon pour l'elution du materiau echantillon a travers le materiau de colonne, ou pour reduire la quantite de solution-tampon.

OPTICAL BIO-DISCS INCLUDING SPIRAL FLUIDIC CIRCUITS FOR PERFORMING ASSAYS

The present invention relates to methods and apparatus for assays including optical bio-discs with spiral fluidic circuits and related detection systems. The optical bio-disc 110 includes a cap portion 116 having inlet and vent ports formed therein, a first channel layer 632 having cut-out portions, a second channel layer 634 having cut-out portions; a third channel layer 636 having cut-cut portions, a fourth channel layer 638 having cut-out portions, and a substantially circular substrate having a center and an outer edge. The cut-out portions are in register with each other such that when the bio-disc 110 is assembled a spiral fluidic circuit is formed having an inlet port, a mixing chamber 134, upper flow chambers 620, lower pass through chambers 622, inlet passages 626, outlet passages 628, a circumferential analysis chamber 618, and vent ports in fluid communication.

Planar centrifugal chromatography device

Detecting abnormal reactions in a red blood cell agglutination

Abnormal reactions in a red blood cell classification by agglutination, are checked following centrifugation of a sample in a column of a cassette, the column containing microparticles. This is done by imaging the column on a detector array that is used to correlate the images with predefined red cell classes based upon the distribution of the images across the column. However, prior to the correlation step, abnormal reactions are checked for by detecting whether any of the following is present: i) errors that cause imaged features of the column or any pellet produced therein to be out of range; ii) hemolysis of the sample; iii) insufficient or too many blood cells present; iv) mixed field agglutination; and v) presence of fibrin at the top of the microparticles.

Vorrichtung zur Oxydation der Abgase von Brennkraftmaschinen

Separation or reaction column unit

A column unit is provided consisting of a centrifuging vessel and a receiving body with feed and discharge openings located at opposite ends. Desired column material is located within a middle portion of a hollow cylinder of the receiving body. A portion of the receiving body containing the discharge opening and the column material is received by the centrifuging vessel. The entire column unit is inserted into a conventional stand in a centrifuge. Accordingly, when sample material is introduced into the receiving body through the feed opening, it may flow without misdirection through the column material and discharge opening and into the centrifuge vessel.

METHODS AND COMPOSITIONS FOR CHROMATOGRAPHY

The present invention is directed to methods and compositions for separating and isolating target molecules. In particular, the present invention comprises devices, such as CCDs, that contain particles without the need of support structures. Chromatography separation techniques, including but not limited to, ion exchange, reversed phase and normal phase partitioning, are used in the CCD. Methods also include low, medium and high pressure liquid chromatography. such methods can be used for analytical, semi-preparative processes, initial clarification, preparative filtration and process scale applications.

TREATMENT APPARATUS

Integrated protein affinity capture centrifugation device

A method and apparatus are described for screening a proteome comprising an array of affinity elements, whereby a proteome is directed through the affinity array and those components that specifically bind to the affinity array are eluted in a suitable eluent by means of centrifugation. Suitable eluents can include a proteome component, a chemical library component or an affinity element. This method and apparatus can be used for several purposes, including screening for bio-active compounds, determining physiological targets of known drugs, determining specificity of compound interactions with physiological targets, and for quantitative protein analyses.

SEPARATION TOWER AND REACTION TOWER APPARATUS

PURPOSE: To make it possible to separate sample material even if the feed quantity of the sample material is slight by inserting a hollow cylindrical receiving body for an in-column material into a centrifuging vessel and intercoupling both to each other. CONSTITUTION: The hollow cylindrical receiving body 2 for the in-column material 4 is provided with a feed opening 8 for the sample material at one end 6 and is disposed with a discharge opening 12 for the sample material after passage through the in-column material 4 at the other end 10. The centrifuging vessel 14, which is housed together with the receiving body 2 in the centrifugal machine and is capable of hermetically receiving the end 10 having the discharge opening 12 of the receiving body 2, is disposed. Consequently, the need for the special fixation of the apparatus is eliminated and the amt. of the sample material past the in-column material in this use is not increased at all (or just 2 to 3 times the feed rate after several ...

TRENN- ODER REAKTIONSSAEULENEINHEIT

SEPARATION OR REACTION COLUMN UNIT

DETECTING ABNORMAL REACTIONS IN A RED BLOOD CELL AGGLUTINATION

Abnormal reactions in a red blood cell classification by agglutination, are checked following centrifugation of a sample in a column of a cassette, the column containing microparticles. This is done by imaging the column on a detector array that is used to correlate the images with predefined red cell classes based upon the distribution of the images across the column. However, prior to the correlation step, abnormal reactions are checked for by detecting whether any of the following is present: i) errors that cause imaged features of the column or any pellet produced therein to be out of range; ii) hemolysis of the sample; iii) insufficient or too many blood cells present; iv) mixed field agglutination; and v) presence of fibrin at the top of the microparticles.

METHOD AND APPARATUS FOR ADSORPTION DETECTION

A method and apparatus for adsorption and detection of adsorbents or analytes. The method and apparatus can be employed in the field for rapid adsorption of analytes and is particularly useful for detection of mycotoxins. A sample to be analyzed is prepared in solution and placed in a test tube. A tube-like adsorption column having a seal and a valve member is forcefully fed into the test tube to force solutions through the valve member into the column and through a filter and adsorbent to trap interferences. The semi-purified solution may then be analyzed for the presence of analytes. The column with the purified solution may be further employed with a second smaller adsorption column similarly equipped with a seal and valve member fitting within the first column. In similar fashion the second column may be forced into the first column to expel the solution therein into the second column and through one or more selective adsorbents for different analytes such as one or more mycotoxins.

METHOD FOR PREPARATION OF SUGAR CHAIN SAMPLE, SUGAR CHAIN SAMPLE, AND METHOD FOR ANALYSIS OF SUGAR CHAIN

A method of preparing a sugar chain sample, for reducing unreacted labeling reagent in a sample solution containing a labeled sugar chain, includes (process 1) a process of bringing the sample solution containing the labeled sugar chain into contact with monolithic silica, so as to allow the monolithic silica to adsorb a sugar chain component; (process 2) a process of washing the monolithic silica with a washing liquid; and (process 3) a process of bringing the monolithic silica into contact with an eluant, so as to elute the adsorbed sugar chain.

VACUUM-ASSISTED AFFINITY CHROMATOGRAPHY APPARATUS AND METHOD

PROBLEM TO BE SOLVED: To provide an apparatus and a method which use a large quantity of samples, effectively, using proper quantity of a medium and quickly separating or purifying the sample for turning it into high quality. SOLUTION: A sample holder 20 constitutes a housing, having an opened upper end 21, and the bottom part is matched with a collar 25, and it and the bottom part are made to match each other so as to form an integral unit. The collar is matched with a vacuum manifold 12 at the annular shoulder. The inner annular ring is attached to a protrusion 27 of the manifold 12. An adapter 33 seals the periphery of a filtering apparatus 30. The filtering apparatus 30 has a form of a single-piece housing. The upper part of the housing is amply large so as to receive the required quantity of extraction. A sample chamber 38 is demarcated, which has an overall cylindrical form for receiving the chromatographic medium and converged downwardly. The end of a lower part 39 of the housing ...

Microchromatographic device and method for rapid determination of a desired substance

A combination of a microcolumn packed with a suitable absorbent material and a centrifuge tube. A predetermined volume of an eluent being capable of selectively eluting a desired substance through the microcolumn is contained above the packing of the absorbent material. The microcolumn and the centrifuge tube are dimensioned in such a manner that upon centrifugation of the predetermined volume of the eluent through the microcolumn, a level of the eluent remaining in the microcolumn, and a level of the eluent in the centrifuge tube coincide substantially at or above a top surface of the packing of the absorbent material. Consequently, centrifugation of the assembly of the microcolumn and the centrifuge tube causes passage of the predetermined volume of eluent through the microcolumn. Determination or assay of the selectively eluted desired substance may be performed by spectrophotometric methods. The device and method is particularly adapted for the determination or assay of a relative concentration ...

SEPARATION OR REACTION COLUMN UNIT

Ionic liquid separations

TYPE-XLL CROSS-AXIS SYNCHRONOUS FLOW-THROUGH COIL PLANET CENTRIFUGE FOR SEPARATION OF BIOPOLYMERS

APPARATUS FOR PURIFYING NUCLEIC ACID AND METHOD OF PURIFYING NUCLEIC ACID

It is intended to provide an apparatus for purifying a nucleic acid which can be easily automated and by which a high contact frequency can be achieved between a nucleic acid in a specimen with a solid phase in the step of capturing the nucleic acid even at a low nucleic acid concentration, thereby establishing a high nucleic acid capturing efficiency. This apparatus for purifying chemicals containing a nucleic acid is provided with means of separating a solution containing the nucleic acid from a sample due to centrifugal force; means of supplying a reagent due to centrifugal force; means of mixing the reagent supplied due to centrifugal force with the solution containing the nucleic acid to give a liquid mixture; a support for capturing the nucleic acid; means of passing the liquid mixture through the support due to centrifugal force; means of passing a reagent different from the above reagent trough the support due to centrifugal force; means of heating the support; and means of holding ...

DEVICES AND METHODS FOR THE PURIFICATION, ISOLATION, DESALTING OR BUFFER/SOLVENT EXCHANGE OF SUBSTANCES

A spin column device, which contains a rigid porous filter that retains its shape during centrifugation, chromatography methods using the device to isolate a desired substance, e.g., a biological molecule, from other substances in a mixture, and kits containing the device with one or more reagents for use in the method.

Methods and apparatus for centrifugal liquid chromatography

Apparatus and methods related to centrifugal liquid chromatography are described. An angular velocity can be simultaneously imparted to a large number of chromatographic enclosures. Via centrifugal forces, a mobile phase fluid including a sample can be driven through a stationary phase within the chromatographic enclosure to perform a chromatographic separation process on components of the sample. The use of centrifugation as a driving force can allow significantly smaller stationary phase particles to be employed as compared to high performance liquid chromatography (HPLC). Further, for an equivalent chromatographic separation process, the use of centrifugation can provide much greater separation efficiencies than HPLC.

Improvements in or relating to chromatographic adsorption processes for the separation and purification of materials

... Chromatographic adsorption apparatus for the separation or purification of substances has the adsorbent arranged in an annulus &c. through which the solution is passed either inwardly or outwardly. It may consist either of loose granules or a coherent body, e.g., a series of superposed discs 131-133 separated by watertight discs 60, 61 and arranged in a perforated annular vessel 2, 4. The vessel may be rotatable to increase the flow by centrifugal action.

IMPROVEMENTS IN LIQUID OR GAS CHROMATOGRAPHY

... 1,202,441. Chromatographic separation process. V. PRETORIUS and H. H. HAHN. 2 Aug., 1967 [5 Aug., 1966], No. 35463/67. Headings B1L and B1X. In a gas or liquid chromatographic separation process distortion of the front of the forwarding phase is prevented by subjecting the forwarding phase to flow velocity profile straightening effects. This can be done (a) by exposing the retarding phase on the pore surfaces of a porous mass of statistically uniform pore size at least in a direction substantially at right angles to the flow path (b) by feeding the forwarding phase through the porous retarding phase under turbulant flow conditions or (c) by feeding the forwarding phase alternatingly through sections of the porous retarding phase and open connecting passages wherein a net transverse flow of the forwarding phase is brought about during flow through the said open connecting passages. The retarding phase comprises or is exposed on the surface of one or more separated porous masses and each ...

CENTRIFUGAL FAST CHROMATOGRAPH

CENTRIFUGAL FAST CHROMATOGRAPH

A centrifugal fast chromatograph is disclosed. The apparatus includes a rotor, a rotor drive, a plurality of chromatograhic columns mounted on the rotor, a transfer disk mounted on the rotor, photodetecting apparatus, a gradient maker and a microprocessor control system. The microprocessor controls and monitors the entire chromatography process. The centrifugal fast chromatograph permits the performance of multiple, rapid, and simultaneous separations.

Separating substance mixtures, e.g. in desalination of sea water, comprises use of column containing solid or gel-like stationary phase and mobile phase

Separating substance mixtures comprises rotating a column containing a solid or gel-like stationary phase and a mobile phase about its longitudinal axis; applying the substance mixture and the mobile phase to one end in an annular region between the rotation center point and the outer edge of the column; transporting through the stationary phase under the action of forces acting parallel to the column axis; and allowing individual fractions of the substance mixture to exit at different points of the column. An Independent claim is also included for a device for carrying out the process. Preferred Process: The mobile phase is liquid or gaseous. The stationary phase contains gradients which are longitudinal and/or transverse to the axis of rotation. The forces acting on the column axis are gravitational force, pressure or a magnetic or electrical field.

Improvements in centrifugal chromatography

... 956,016. Centrifugal chromatography. MALVERN INSTITUTE FOR PSYCHIATRIC & ALCOHOLIC STUDIES. Aug. 18, 1961 [Dec. 21, 1960], No. 29891/61. Heading B1X. A chromatographic separating device includes a rotating mechanism having an axis of rotation and supporting a rotor bearing a channel which extends radially to the said rotating mechanism and has lateral defining walls, a separating medium positioned in the channel, and a device to supply developing liquid to the separating medium at a radially inner part thereof. As shown in Figs. 3 and 4, a disc 20 is mounted on a shaft 21 turned on a vertical axis by a motor whose shaft mounts on adapter 23 secured to the disc 20. Above the stub shaft portion 26 the adapter has an extension 28 and threaded end-portion 30, which have a central opening 31 to receive development liquid from a controllable feed device 32. The central opening 31 communicates with a plurality of radially extending parts 33 leading to a distributer cup 35 and to parts 41 aligned ...

IMMUNOAFFINITY COLUMN FOR THE SIMULTANEOUS EXTRACTION OF SEVERAL ANABOLIC HORMONE RESIDUES FROM BIOLOGICAL LIQUIDS AND THE METHOD FOR ITS PREPARATION

Ionic liquid separations

A process for the separation of organic compounds comprises centrifugal partitioning of at least one organic compound between a mobile liquid phase and an immiscible stationary liquid phase. At least one of the mobile liquid phase and the stationary liquid phase comprises or consists of an ionic liquid. A rotary coil centrifuge for counter current chromatography and a liquid-liquid chromatography or liquid-liquid extraction apparatus may be used in the process.

Improvements in or relating to chromatographic separation

An apparatus for chromatographically separating substances from one another comprises a container, a material in the container which is chromatographically active with respect to at least one of the substances, and means for mechanically segregating a region of the material from the remainder thereof. An apparatus intended for radial chromatography (Fig. 2) comprises a chamber 7 filled with chromatographically active material, an annular portion 10 thereof in which the recovered concentrates being separated from the remainder by two coaxial cylindrical perforated walls 11 and 12 interconnected by a removable bottom 13 and a removable lid 23. The bottom 13 stands on a support 14 carried by jacks 15. The material is recovered by unlocking the lid 23 and raising the jacks 15, whereby the chamber containing the annular portion 10 is raised to a position above the chamber 7 where it can be replaced by another charged chamber. A plurality of such removable ...

CPC DISTRIBUTION CHROMATOGRAPHY OF CANNABINOIDS

The invention relates to cannabinoids and their isolation and purification and to obtaining them by means of centrifugal partition chromatography. 111.-. (canceled)12. A method for separating and/or purifying cannabinoids from a cannabis extract , including at least one liquid-liquid centrifugal partition chromatography step , wherein a solvent from the group of cyclohexane , heptane , or octane is selected and is kept stationary by centrifugal force , including and a second non-miscible liquid phase.13. The method for separating and/or purifying cannabinoids from a cannabis extract according to claim 12 , wherein the first phase is n-heptane.14. The method for separating and/or purifying cannabinoids from a cannabis extract according to claim 12 , wherein the second phase is acetonitrile claim 12 , methanol claim 12 , ethylacetate or water.15. The method for separating and/or purifying cannabinoids from a cannabis extract according to claim 12 , selected from the group consisting of:n-heptane/acetonitrile, n-heptane/ethylacetate/acetonitrile, n-heptane/ethylacetate/t-butyl methyl ether/acetonitrile,n-heptane/ethylacetate/methanol/water,n-heptane/methanol/water, n-heptane/ethanol/water,n-heptane/acetone/water, n-heptane/methanol/acetonitrile,n-heptane/ethanol/acetonitrile,n-heptane/acetone/acetonitrile,n-heptane/chloroform/acetonitrile,n-heptane/chloroform/methanol, n-heptane/methanol,n-heptane/ethanol/methanol,n-heptane/n-butanol/acetonitrile, n-heptane/2-propanol/water, n-heptane/n-propanol/water, n-heptane/2-propanol/acetonitrile, n-heptane/n-propanol/acetonitrile, n-heptane/dichloromethane/acetonitrile,n-heptane/dichloromethane/methanol,n-heptane/tetrahydrofuran/acetonitrile,n-heptane/benzotrifluoride/acetonitrile, Cyclohexane/methanol/water,Cyclohexane/methanol/acetonitrile,Cyclohexane/t-butyl methyl ether/water, Cyclohexane/acetonitrile/water, andIsooctane/methanol, isooctane/methanol/water, Isooctane/ethyl acetate/methanol/water.16. The method for separating ...

CPC DISTRIBUTION CHROMATOGRAPHY OF CANNABINOIDS

The invention relates to cannabinoids and their isolation and purification and to obtaining them by means of centrifugal partition chromatography. 1. A method for separating and/or purifying cannabinoids from a cannabis extract , including at least one liquid-liquid centrifugal partition chromatography step , wherein a solvent from the group of cyclohexane , heptane , or octane is selected and is kept stationary by centrifugal force , including and a second non-miscible liquid phase.2. The method for separating and/or purifying cannabinoids from a cannabis extract according to claim 1 , wherein the first phase is n-heptane.3. The method for separating and/or purifying cannabinoids from a cannabis extract according to claim 1 , wherein the second phase is acetonitrile claim 1 , methanol claim 1 , ethylacetate or water.4. The method for separating and/or purifying cannabinoids from a cannabis extract according to claim 1 , selected from the group:n-heptane/acetonitrile,n-heptane/ethylacetate/acetonitrile,n-heptane/ethylacetate/t-butyl methyl ether/acetonitrile,n-heptane/ethylacetate/methanol/water,n-heptane/methanol/water, n-heptane/ethanol/water,n-heptane/acetone/water, n-heptane/methanol/acetonitrile,n-heptane/ethanol/acetonitrile,n-heptane/acetone/acetonitrile,n-heptane/chloroform/acetonitrile,n-heptane/chloroform/methanol, n-heptane/methanol,n-heptane/ethanol/methanol,n-heptane/n-butanol/acetonitrile, n-heptane/2-propanol/water, n-heptane/n-propanol/water, n-heptane/2-propanol/acetonitrile, n-heptane/n-propanol/acetonitrile,n-heptane/dichloromethane/acetonitrile,n-heptane/dichloromethane/methanol,n-heptane/tetrahydrofuran/acetonitrile,n-heptane/benzotrifluoride/acetonitrile,Cyclohexane/methanol/water,Cyclohexane/methanol/acetonitrile,Cyclohexane/t-butyl methyl ether/water,Cyclohexane/acetonitrile/water,Isooctane/methanol, isooctane/methanol/water,Isooctane/ethyl acetate/methanol/water.5. The method for separating and/or purifying cannabinoids from a cannabis ...

Methods for pathogen detection

The present disclosure relates to methods, systems, devices, and microfluidic chips that may be used for the detection of pathogens.

Devices and methods for the purification, isolation, desalting or buffer/solvent exchange of substances

一种旋转柱装置，包含刚性多孔过滤器，该刚性多孔过滤器在离心分离过程中仍保持其形状；层析方法，使用上述装置将所需物质例如生物分子从混合物中的其它物质中分离；以及套件，包含具有用于上述方法中的一种或多种试剂的装置。

A chemical material refining structure

본 발명은, 자동화가 용이하고 핵산의 농도가 저농도인 경우에도 핵산 포착 공정에서 핵체 중의 핵산과 고상과의 접촉 빈도를 높기 때문에 핵산의 포착율이 높은 핵산 자동 정제 장치를 제공한다. 그 때문에, 핵산을 포함하는 화학 물질의 정제 장치는, 원심력으로 핵산을 포함하는 용액을 시료로부터 분리시키는 수단과, 원심력으로 시약을 송액시키는 수단과, 원심력으로 송액시킨 시약과 핵산을 포함하는 용액을 혼합시켜 혼합액을 만드는 수단과, 핵산을 포착하기 위한 담체와, 혼합액을 담체에 원심력에 의해 통액시키는 수단과, 원심력으로 상기 시약과는 다른 시약을 상기 담체에 통액시키는 수단과, 담체를 가열하는 수단과, 상기 담체로부터 용리된 핵산을 함유하는 시약을 다른 원심력에 의해 다른 시약과 구별하여 보유할 수 있는 보유 수단을 구비한다.  The present invention provides a nucleic acid automatic purification device having a high rate of capturing nucleic acid because the frequency of contact between the nucleic acid in the nucleus and the solid phase is high in the nucleic acid capturing step even when the automation is easy and the concentration of the nucleic acid is low. Therefore, the purification apparatus of the chemical substance containing a nucleic acid provides the means which isolate | separates the solution containing a nucleic acid from a sample by centrifugal force, the means which conveys a reagent by centrifugal force, the solution containing the reagent and nucleic acid which were sent by centrifugal force, Means for mixing to form a mixed solution, a carrier for capturing nucleic acids, a means for passing the mixed solution through the carrier by centrifugal force, a means for passing a reagent different from the reagent by the centrifugal force into the carrier, and a means for heating the carrier. And a holding means capable of holding a reagent containing a nucleic acid eluted from the carrier different from other reagents by another centrifugal force. 핵산 정제 장치, 핵산 정제 구조체, 유전자 분석 장치  Nucleic Acid Purification Device, Nucleic Acid Purification Structure, Genetic Analysis Device

Apparatus for effecting interactions of fluids at extended solid surfaces

Devices and methods for the purification, isolation, desalting or buffer/solvent exchange of substances

A spin column device, which contains a rigid porous filter that retains its shape during centrifugation, chromatography methods using the device to isolate a desired substance, e.g., a biological molecule, from other substances in a mixture, and kits containing the device with one or more reagents for use in the method.

Detecting abnormal reactions in a red blood cell agglutination

Abnormal reactions in a red blood cell classification by agglutination, are checked following centrifugation of a sample in a column of a cassette, the column containing microparticles. This is done by imaging the column on a detector array that is used to correlate the images with predefined red cell classes based upon the distribution of the images across the column. However, prior to the correlation step, abnormal reactions are checked for by detecting whether any of the following is present: i) errors that cause imaged features of the column or any pellet produced therein to be out of range; ii) hemolysis of the sample; iii) insufficient or too many blood cells present; iv) mixed field agglutination; and v) presence of fibrin at the top of the microparticles.

Device for the treatment of biomolecules

Devices and methods for the purification, isolation, desalting or buffer/solvent exchange of substances

一种旋转柱装置，包含刚性多孔过滤器，该刚性多孔过滤器在离心分离过程中仍保持其形状；层析方法，使用上述装置将所需物质例如生物分子从混合物中的其它物质中分离；以及套件，包含具有用于上述方法中的一种或多种试剂的装置。

Devices and methods for the purification, isolation, desalting or buffer/solvent exchange of substances

Vacuum assisted affinity chromatography device and method

Sample preparation device and method particularly useful for large volume capture and small volume elution. The device comprising a manifold, a sample holder or reservoir, and a filter unit containing chromatography media such that a filtration path is established between the sample holder, the filter unit and the manifold. Upon subjecting the sample in the sample holder to a driving force such as vacuum, the sample flows through the chromatography media in the filter unit. Molecules of interest bind to the media and any unwanted molecules can be washed under vacuum mode. Elution can then be carried out by removing the filter unit and subjecting the media to a driving force such as vacuum or centrifugation. In another embodiment, molecules of interest can be eluted into a low volume centrifugal spinner directly in the manifold.

Centrifugal liquid chromatography

(57)【要約】本公報は電子出願前の出願データであるた め要約のデータは記録されません。

Device and method for placing immiscible fluid phases in contact with each other by means of centrifugal force

Apparatus for Purifying Nucleic Acid and Method of Purifying Nucleic Acid

본 발명은, 자동화가 용이하고 핵산의 농도가 저농도인 경우에도 핵산 포착 공정에서 핵체 중의 핵산과 고상과의 접촉 빈도를 높기 때문에 핵산의 포착율이 높은 핵산 자동 정제 장치를 제공한다. 그 때문에, 핵산을 포함하는 화학 물질의 정제 장치는, 원심력으로 핵산을 포함하는 용액을 시료로부터 분리시키는 수단과, 원심력으로 시약을 송액시키는 수단과, 원심력으로 송액시킨 시약과 핵산을 포함하는 용액을 혼합시켜 혼합액을 만드는 수단과, 핵산을 포착하기 위한 담체와, 혼합액을 담체에 원심력에 의해 통액시키는 수단과, 원심력으로 상기 시약과는 다른 시약을 상기 담체에 통액시키는 수단과, 담체를 가열하는 수단과, 상기 담체로부터 용리된 핵산을 함유하는 시약을 다른 원심력에 의해 다른 시약과 구별하여 보유할 수 있는 보유 수단을 구비한다. The present invention provides a nucleic acid automatic purification device having a high rate of capturing nucleic acid because the frequency of contact between the nucleic acid in the nucleus and the solid phase is high in the nucleic acid capturing step even when the automation is easy and the concentration of the nucleic acid is low. Therefore, the purification apparatus of the chemical substance containing a nucleic acid provides the means which isolate | separates the solution containing a nucleic acid from a sample by centrifugal force, the means which conveys a reagent by centrifugal force, the solution containing the reagent and nucleic acid which were sent by centrifugal force, Means for mixing to form a mixed solution, a carrier for capturing nucleic acids, a means for passing the mixed solution through the carrier by centrifugal force, a means for passing a reagent different from the reagent by the centrifugal force into the carrier, and a means for heating the carrier. And a holding means capable of holding a reagent containing a nucleic acid eluted from the carrier different from other reagents by another centrifugal force. 핵산 정제 장치, 핵산 정제 구조체, 유전자 분석 장치 Nucleic Acid Purification Device, Nucleic Acid Purification Structure, Genetic Analysis Device

Preparative separation of fluid samples

A method and apparatus for preparative separation of the components of a fluid sample as a result of differential migration through a porous medium in which the porous medium is in the form of an annulus to the inner surface of which both the fluid sample and a fluid carrier for the sample are supplied continuously and the separated components are collected continuously from the outer surface of the annulus as the result of relative rotation preferably produced by causing the annulus to rotate at a steady speed while keeping the point of application of the sample and a collection arrangement at the outer surface of the annulus stationary.

Centrifugal solvent extraction

Integrated protein affinity capture centrifugation device

A method and apparatus are described for screening a proteome. The method and apparatus include an array of affinity elements, such that a proteome is directed through the array and those components that specifically bind to the affinity elements of the array are eluted in a suitable eluent by means of centrifugation. Suitable eluents can include a proteome component, a chemical library component or an affinity element. This method and apparatus can be used for several purposes, including screening for bio-active compounds, determining physiological targets of known drugs, determining specificity of compound interactions with physiological targets, and for quantitative protein analyses.

Vacuum assisted affinity chromatography device and method

Sample preparation device and method particularly useful for large volume capture and small volume elution. The device comprising a manifold, a sample holder or reservoir, and a filter unit containing chromatography media such that a filtration path is established between the sample holder, the filter unit and the manifold. Upon subjecting the sample in the sample holder to a driving force such as vacuum, the sample flows through the chromatography media in the filter unit. Molecules of interest bind to the media and any unwanted molecules can be washed under vacuum mode. Elution can then be carried out by removing the filter unit and subjecting the media to a driving force such as vacuum or centrifugation. In another embodiment, molecules of interest can be eluted into a low volume centrifugal spinner directly in the manifold.

A chemical material refining structure

본 발명은, 자동화가 용이하고 핵산의 농도가 저농도인 경우에도 핵산 포착 공정에서 핵체 중의 핵산과 고상과의 접촉 빈도를 높기 때문에 핵산의 포착율이 높은 핵산 자동 정제 장치를 제공한다. 그 때문에, 핵산을 포함하는 화학 물질의 정제 장치는, 원심력으로 핵산을 포함하는 용액을 시료로부터 분리시키는 수단과, 원심력으로 시약을 송액시키는 수단과, 원심력으로 송액시킨 시약과 핵산을 포함하는 용액을 혼합시켜 혼합액을 만드는 수단과, 핵산을 포착하기 위한 담체와, 혼합액을 담체에 원심력에 의해 통액시키는 수단과, 원심력으로 상기 시약과는 다른 시약을 상기 담체에 통액시키는 수단과, 담체를 가열하는 수단과, 상기 담체로부터 용리된 핵산을 함유하는 시약을 다른 원심력에 의해 다른 시약과 구별하여 보유할 수 있는 보유 수단을 구비한다.

Apparatus and process for treating a fluid material while it is subjected to a centrifugal force

Apparatus for treating a fluid material which apparatus comprises (a) a permeable element (22, 23) at least a portion of which is permeable to the fluid material and or, where the fluid material is treated with a fluid in apparatus according to the invention, to the fluid, which permeable element is rotatable about an axis, (b) means (28,29) to charge the fluid material to the permeable element and (c) means (31) to discharge the fluid material or a component or derivative thereof from the permeable element characterised in that the apparatus is adapted to discharge the fluid material or a component or derivative thereof from the permeable element axially distant the position at which the fluid material is charged thereto.

Method for Pre-Fractionation of Complex Samples

The present invention relates to a method for pre-fractionation of complex protein and/or peptide samples. The method comprises the following steps: a) loading the sample onto pre-packed media able to separate proteins/peptides according to their pl-value; b) eluting the media by centrifugal force under denaturing conditions with at least three buffers having different pH in a stepwise manner to obtain at least three fractions separated according to pl-value; and c) subjecting each fraction to further separation in a pH-range corresponding to the pl-values of said fractions. The invention also relates to a kit comprising at least one spin column or multiwell plate filled with chromatography medium able to separate proteins/peptides according to pl-value, at least three buffers, and one or more IPG strips having a narrow pH-range adapted to each of said buffers.

Devices and methods for purification

The present invention provides a device and method for the purification and separation of substances. The purification device comprises a sample holder comprising a sample chamber and a column module. The column module is securable to the sample holder and is packed with chromatography medium having a special affinity for attracting a given substance. A variety of mediums can be used in the capsule of the purification device, each containing different medium types. The column modules may be prepacked by the manufacturer or may be provided empty so that a user defined medium may be inserted into the capsule by the user. With the column module and sample loaded, pressure may be applied to move the sample through the module to by means such as centrifuging in order to separate the selected substance from the sample.

Métodos para purificar anticorpos

métodos para purificar anticorpos. a invenção se refere ao campo de fabricar moléculas de anticorpo recombinantes. em particular, métodos para purificar tais moléculas de anticorpo recombinantes são providas em que imidazol ou um análogo de imidazol é adicionado durante a elução da molécula de anticorpo recombinante a partir de uma resina de cromatografia por afinidade, tal como uma resina com base em proteína a.

Nachweis von abnormen reaktionen in einer erythrozyten-agglutination

Centrifugal-driven microfluidic platform and method of use thereof

In this invention, chromatography is integrated on a centrifugal platform to enable low-cost automated purification. Differing from the traditional chromatography method, purification and separation of a centrifugal compound collecting platform disclosed in the present invention mainly uses a centrifugal force to drive the fluid to flow outward in the radial direction when the motor rotates. The compounds to be separated react with the column packing during the flow, and the compounds with different polarities in the sample are gradually separated. The flow of the fluid can be governed by the motor and the geometry of the fluidic design such that compounds with different characteristics can be separated and collected in different collecting chambers.

遠心式液体クロマトグラフ

Devices and methods for purification

The present invention provides a device and method for the purification and separation of substances. The purification device comprises a sample holder (1) comprising a sample chamber (22) and a column module (5). The column module (5) is securable to the sample holder (1) and is packed with chromatography medium (60) having a special affinity for attracting a given substance. A variety of mediums can be used in the capsule of the purification device wherein each contains different medium types. The column modules (5) may be prepacked by the manufacturer or may be provided empty so that a user defined medium may be inserted into the capsule by the user. With the column module (5) and sample loaded, pressure may be applied to move the sample through the module (5) to by means such as centrifuging in order to separate the selected substance from the sample.

Method for pre-fractionation of complex samples

The present invention relates to a method for pre-fractionation of complex protein and/or peptide samples. The method comprises the following steps: a) loading the sample onto pre-packed media able to separate proteins/peptides according to their pI-value; b) eluting the media by centrifugal force under denaturing conditions with at least three buffers having different pH in a stepwise manner to obtain at least three fractions separated according to pI-value; and c) subjecting each fraction to further separation in a pH-range corresponding to the pI-values of said fractions. The invention also relates to a kit comprising at least one spin column or multiwell plate filled with chromatography medium able to separate proteins/peptides according to pI-value, at least three buffers, and one or more IPG strips having a narrow pH-range adapted to each of said buffers.

Method for pre-fractionation of complex samples

The present invention relates to a method for pre-fractionation of complex protein and/or peptide samples. The method comprises the following steps: a) loading the sample onto pre-packed media able to separate proteins/peptides according to their pI-value; b) eluting the media by centrifugal force under denaturing conditions with at least three buffers having different pH in a stepwise manner to obtain at least three fractions separated according to pI-value; and c) subjecting each fraction to further separation in a pH-range corresponding to the pI-values of said fractions. The invention also relates to a kit comprising at least one spin column or multiwell plate filled with chromatography medium able to separate proteins/peptides according to pI-value, at least three buffers, and one or more IPG strips having a narrow pH-range adapted to each of said buffers.

Cpc verteilungschromatographie von cannabinoiden

Die Erfindung betrifft Cannabinoide und deren Isolierung und Aufreinigung als auch Gewinnung mittels Centrifugal Partition Chromatography, wobei ein Lösungsmittel aus der Gruppe Cyclohexan, Heptan oder Octan ausgewählt ist, das durch Zentrifugalkraft stationär gehalten wird und eine zweite nicht mischbare flüssige Phase.

糖鎖試料調製方法

【課題】糖鎖ラベル化反応後、過剰のラベル化試薬を効率よく除去する手段を提供すること。【解決手段】ラベル化糖鎖を含む試料溶液中の未反応のラベル化試薬を低減させるための糖鎖試料調製方法であって、（工程１）ラベル化糖鎖を含む試料溶液をモノリスシリカと接触させることにより、モノリスシリカに糖鎖成分を吸着させる工程、（工程２）モノリスシリカを洗浄液で洗浄する工程、（工程３）モノリスシリカに溶出液を接触させ、吸着した糖鎖成分を溶出させる工程、を含むことを特徴とする糖鎖試料調製方法。【選択図】なし

Cpc verteilungschromatographie von cannabinoiden

Die Erfindung betrifft Cannabinoide und deren Isolierung und Aufreinigung als auch Gewinnung mittels Centrifugal Partition Chromatography, wobei ein Lösungsmittel aus der Gruppe Cyclohexan, Heptan oder Octan ausgewählt ist, das durch Zentrifugalkraft stationär gehalten wird und eine zweite nicht mischbare flüssige Phase.

離心式純化平台及其使用方法

在這項發明中，層析法被整合到離心平台上，以實現低成本的自動純化。與傳統層析方法不同，本發明中提出之一種離心式化合物純化及分離蒐集平台，主要利用馬達旋轉時，流體受到離心力驅動往外半徑流動，待分離樣本在流動的過程中與填充物反應，樣本在流動的過程中逐漸分離出不同極性的化合物，馬達能控制流體流速使碟片上之流體在微型管柱層析結構中達到純化並且完成後續完成分離及蒐集之動作。

Centrifugal liquid chromatograph

Cpc verteilungschromatographie von cannabinoiden

Die Erfindung betrifft die Isolierung und Aufreinigung von Cannabinoiden mittels Centrifugal Partition Chromatography.

クロマトグラフィー用の方法及び組成物

本発明は、標的分子を分離及び単離するための方法及び組成物に関する。特に、本発明は、ＣＣＤのような、粒子を含み支持構造を必要としない装置を含む。ＣＣＤにおいて、限定はされないが、イオン交換、逆相及び純相分配を含む、クロマトグラフィー分離技術が用いられる。これらの方法は、低圧、中圧及び高圧液体クロマトグラフィーも含む。このような方法は、分析的、準分取プロセス、初期清澄化、分取濾過及びプロセス基準化用途に用いることができる。

糖鎖試料調製方法、糖鎖試料および糖鎖分析法

ラベル化糖鎖を含む試料溶液中の未反応のラベル化試薬を低減させるための糖鎖試料調製方法であって、（工程１）ラベル化糖鎖を含む試料溶液をモノリスシリカと接触させることにより、モノリスシリカに糖鎖成分を吸着させる工程、（工程２）モノリスシリカを洗浄液で洗浄する工程、（工程３）モノリスシリカに溶出液を接触させ、吸着した糖鎖成分を溶出させる工程、を含むことを特徴とする糖鎖試料調製方法。

Métodos para purificar anticuerpos

La presente se refiere al campo de la producción de moléculas de anticuerpos recombinantes. En particular, se proporcionan métodos para purificar tales moléculas de anticuerpo recombinante en las que se agrega imidazol o un análogo de imidazol durante la elución de la molécula de anticuerpo recombinante a partir de una resina de cromatografía de afinidad, tal como una resina basada en proteína A.

Method for separating and producing a purified PCBM

Ionic liquid separations

A process for the separation of chemical compounds comprises centrifugal partitioning of at least one compound between a mobile liquid phase and an immiscible stationary liquid phase. At least one of the mobile liquid phase and the stationary liquid phase comprises or consists of an ionic liquid. A liquid-liquid chromatography or liquid-liquid extraction apparatus may be used in the process.

Verbesserungen in der Chromatographie

Verfahren zur durchfuehrung analytischer bestimmungen und hierfuer geeignetes rotoreinsatzelement.

Methods and apparatus for centrifugal liquid chromatography

Apparatus and methods related to centrifugal liquid chromatography are described. An angular velocity can be simultaneously imparted to a large number of chromatographic enclosures. Via centrifugal forces, a mobile phase fluid including a sample can be driven through a stationary phase within the chromatographic enclosure to perform a chromatographic separation process on components of the sample. The use of centrifugation as a driving force can allow significantly smaller stationary phase particles to be employed as compared to high performance liquid chromatography (HPLC). Further, for an equivalent chromatographic separation process, the use of centrifugation can provide much greater separation efficiencies than HPLC.

Methods and apparatus for centrifugal liquid chromatography

Apparatus and methods related to centrifugal liquid chromatography are described. An angular velocity can be simultaneously imparted to a large number of chromatographic enclosures. Via centrifugal forces, a mobile phase fluid including a sample can be driven through a stationary phase within the chromatographic enclosure to perform a chromatographic separation process on components of the sample. The use of centrifugation as a driving force can allow significantly smaller stationary phase particles to be employed as compared to high performance liquid chromatography (HPLC). Further, for an equivalent chromatographic separation process, the use of centrifugation can provide much greater separation efficiencies than HPLC.

Methods and apparatus for centrifugal liquid chromatography

Apparatus and methods related to centrifugal liquid chromatography are described. An angular velocity can be simultaneously imparted to a large number of chromatographic enclosures. Via centrifugal forces, a mobile phase fluid including a sample can be driven through a stationary phase within the chromatographic enclosure to perform a chromatographic separation process on components of the sample. The use of centrifugation as a driving force can allow significantly smaller stationary phase particles to be employed as compared to high performance liquid chromatography (HPLC). Further, for an equivalent chromatographic separation process, the use of centrifugation can provide much greater separation efficiencies than HPLC.

Novel type of extraction cell for a centrifugal partition chromatograph, as well as a centrifugal partition chromatograph containing such an extraction cell

The object of the invention relates to an extraction cell ( 100 ) used in a centrifugal partition chromatograph, which has a cell wall ( 120 ) determining a closed extraction chamber ( 150 ), as well as an inlet ( 115 ) and an outlet ( 140 ) ensuring the fluid connection between the extraction chamber ( 150 ) and the space outside of the extraction cell ( 100 ) formed on essentially opposite parts of the cell wall ( 120 ). The extraction cell ( 100 ) according to the invention is constructed asymmetrically from the point of view of the reversibility of the direction of flow used when the centrifugal partition chromatograph is in operation.

Keyed column chromatography apparatus

Keyed column chromatography apparatus comprises a polymer body (30) having a channel (37) extending therethrough, means (31) for coupling the column to insertion apparatus (10) having complementary coupling means (12), and keying means (34) on the outer surface of the column. In addition, a collecting tube (14) comprises a hollow body having one end having an aperture therethrough and having keying means (20) on the outer surface of the tube. Further, a rack (50) for receiving generally cylindrical bodies having complementary keying means comprises a top surface (58) and a plurality of legs (56) sufficient to support the top surface. The kit comprising and facilitating the same method is included.

核酸精制装置以及核酸精制方法

本发明涉及核酸精制装置以及核酸精制方法。提供一种自动化容易，即使在核酸的浓度低时，由于在核酸捕捉工序中检体中核酸和固相的接触频率高，进而核酸的捕捉率高的核酸精制装置。本发明的含有核酸的化学物质的精制装置备有通过离心力从样品中使含有核酸的溶液分离的部件，通过离心力输送试剂的部件，使通过离心力输送的试剂和含有核酸的溶液混合成混合液的部件，用于捕捉核酸的载体、通过离心力将上述混合液流过载体的部件，通过离心力使与先前试剂不同的其他试剂流过上述载体的部件，对载体进行加热的部件，通过离心力将含有从载体上洗脱的核酸的试剂与其他试剂区别后进行保持的部件。

Novel type of extraction cell for a centrifugal partition chromatograph, as well as a centrifugal partition chromatograph containing such an extraction cell

The object of the invention relates to an extraction cell (100) used in a centrifugal partition chromatograph, which has a cell wall (120) determining a closed extraction chamber (150), as well as an inlet (115) and an outlet (140) ensuring the fluid connection between the extraction chamber (150) and the space outside of the extraction cell (100) formed on essentially opposite parts of the cell wall (120). The extraction cell (100) according to the invention is constructed asymmetrically from the point of view of the reversibility of the direction of flow used when the centrifugal partition chromatograph is in operation.

纯化抗体的方法

本发明涉及制造重组抗体分子的领域。特别地，提供了纯化这种重组抗体分子的方法，其中在重组抗体分子从亲和色谱树脂诸如基于蛋白A的树脂中洗脱的过程中加入咪唑或咪唑类似物。