Antreiben von Fluid im Probentrenngerät basierend auf Kondition des Fluids an mehreren Stellen

Steuervorrichtung (70) für ein Probentrenngerät (10), wobei die Steuervorrichtung (70) eine Empfangseinrichtung (152) zum Empfangen von Informationen, die indikativ für mindestens eine Bedingung eines Fluids an mindestens zwei Stellen entlang eines Flusspfades des Fluids in dem Probentrenngerät (10) sind, und eine Steuereinrichtung (154) aufweist, die zum Steuern eines zum Antreiben des Fluids innerhalb des Flusspfades ausgebildeten Fluidantriebs des Probentrenngeräts (10) unter Verwendung der Informationen eingerichtet ist.

Fractionation purification apparatus

A target compound is trapped by an adsorbent within trap column (7) held in approximately vertical uprising form by column rack (8), and the interior thereof is replaced by water for washing. Thereafter, dichloromethane with a specific gravity greater than that of water, not miscible with water, is slowly introduced from entrance edge (7a) at the inferior end by liquid feed pump (5). The water in the trap column (7) is pushed up by the dichloromethane, flowing out from exit edge (7b) and discarded through 2-way switch valve (12) and waste liquid channel (14). As the dichloromethane leaches the trapped target compound, when the eluate from the trap column (7) is changed through water and emulsion to dichloromethane, the target compound of satisfactory concentration is contained in the eluate and thus the 2-way switch valve (12) is switched to fractionation channel (13). Consequently, there can be attained satisfactory removal of water, recovery of the eluate containing the target compound ...

Preparative separation/ purification system

Gas purification apparatus and trace substance detection device

VENTIL UND VERWENDUNG DES VENTILS

The invention relates to a valve, in particular a sample injection valve, for a device (1) for synthesizing, analyzing, and/or separating, comprising at least three liquid connections (3', 4', 5', 6', 7', 7"), a housing (8) as a valve part, and a valve body (9) as another valve part for selectively connecting the liquid connections (3', 4', 5', 6', 7', 7") by means of at least one flow channel (10, 11, 12) bounded at least partially by sealing surfaces (10', 11', 12') between the housing (8) and the valve body (9), wherein the housing (8) and/or the valve body (9) are supported in such a way as to be movable relative to each other. In order to a create a one-way valve, at least one valve part (8 or 9) adjacent to the sealing surface (10', 11', 12') and made of a plastic material can be plastically deformed according to a relative position (9'), in particular of the valve body (9), in order to be able to withstand the elevated pressure loads in the flow channel (10, 11, 12) in a liquid-tight ...

PROTEIN CHROMATOGRAPHY SYSTEM

ANALYSIS METHOD AND ANALYSIS DEVICE FOR SUBSTANCE TO BE MEASURED

An analysis method for a target substance comprising: a step (a) in which a sample is introduced into a flow channel having a bypass flow channel, the sample introduced into the bypass flow channel is introduced into a measurement device, and the signal strength or concentration of a target substance and/or carrier in the sample is measured; a step (b) in which a sample not introduced into the bypass flow channel is introduced into a column and the target substance and/or carrier is adsorbed; and a step (c) in which an eluent is introduced into the column, the target substance and/or carrier that was adsorbed onto the column is eluted and introduced into the measurement device, and the signal strength or concentration of the eluted target substance and/or carrier is measured.

MIKROSAEULEN FLUESSIGKEITSCHROMATOGRAF.

ELECTRON CAPTURE DETECTOR, DETECTION CELL, CLEANING METHOD, ANALYSIS METHOD AND ANALYTICAL DEVICE

The flow path of the bubble reducing device and method, liquid supply device and a chromatography device

本发明提供流路内气泡减少装置、程序以及方法、液体供给装置及色谱装置，其目的在于减少流路内的气泡。设有在第一流路（14）形成空气层的空气层形成单元。通过活塞泵（63）的推操作使所形成的空气层在第一流路（14）内移动，将第一流路（14）内的气泡取入到空气层。

샘플을 분석하고 샘플 분획을 수집하기 위한 방법 및 장치

... 본 발명에는 하나 이상의 검출기를 사용하여 샘플을 분석하는 방법 및 장치가 개시되어 있다.

모사이동층 방식 크로마토그래피 분리 방법 및 모사이동층 방식 크로마토그래피 분리 시스템

A chromatographic separation method using a simulated moving-bed technique, whereby it becomes possible to separate a component that is weakly adsorptive to an adsorbent, i.e., a weakly adsorptive component, a component that is strongly adsorptive to the adsorbent, i.e., a strongly adsorptive component, and a component that is adsorptive to the adsorbent at the level intermediate between the weakly adsorptive component and the strongly adsorptive component, i.e., a moderately adsorptive component, all contained in a base solution with at least two eluents using a circulatory system having such a structure that a plurality of packed unit columns each having the adsorbent filled therein are connected to each other in series and endlessly through a pipe, wherein the pipe in the circulatory system is provided with a base liquid supply port F, at least two eluent supply ports D respectively corresponding to the at least two eluents, an extraction port A for a weakly adsorptive fraction containing ...

CONNECTOR WITH STRUCTURAL REINFORCEMENT AND BIOCOMPATIBLE FLUID PASSAGEWAY

A connector with a biocompatible fluid passageway and method for manufacturing thereof is described. The biocompatible connector may be used in an analytical instrument (AI) system as a union, an adapter, a tee, a cross, a manifold, a valve, a column filter retainer, or for other fittings or components. The connector has a reinforcement insert and a biocompatible molding covering portions of the reinforcement insert. The reinforcement insert has a first portion, a second portion, and a middle portion between the first portion and the second portion. The first and second portions have threaded sections and each have a plurality of non-threaded sections. For a given portion, the junction of the non-threaded sections forms a lip by which to prevent the molded material from flowing into the threaded sections. In certain embodiments, an interior web is used in the reinforcement insert to provide additional structural support.

THIN LAYER CHROMATOGRAPHY PLATE AND SAMPLE ANALYSIS METHOD USING SAME

Provided is a thin layer chromatography plate that makes it possible to separate a plurality of components from each other more simply and more quickly. Thin layer chromatography plate includes substrate and separation layer that is disposed on substrate for separating multiple components included in a sample from each other. Separation layer has first layer that has a band shape and extends in first development direction and second layer that extends in second development direction orthogonal to first development direction. Second layer is in contact with first layer. First layer includes a hydrophilic porous body. Second layer includes a hydrophobic porous body. 1. A thin layer chromatography plate comprising:a substrate; anda separation layer disposed on the substrate, the separation layer separating multiple components included in a sample from each other,whereinthe separation layer has a first layer that has a band shape and extends in a first development direction and a second layer that extends in a second development direction orthogonal to the first development direction,the second layer is in contact with the first layer,the first layer includes a hydrophilic porous body, andthe second layer includes a hydrophobic porous body.2. The thin layer chromatography plate according to claim 1 , whereinthe first layer and the second layer are both disposed on the substrate, anda lateral surface of the first layer and a lateral surface of the second layer are in contact with each other.3. The thin layer chromatography plate according to claim 1 , whereinthe separation layer further has a third layer in contact with the second layer,the first layer, the second layer, and the third layer are arrayed in sequence in the second development direction,the third layer includes a porous body, andat least one requirement selected from among a requirement of a composition of the third layer being different from a composition of the second layer and a requirement of a structure ...

TWO-DIMENSIONAL LIQUID CHROMATOGRAPH SYSTEM

A two-dimensional liquid chromatograph system includes a first dimension analysis part, a second dimension analysis part, a second dimension feeding device, a fraction introduction part, a shift gradient program creation part, a liquid feeding control part, and a shift timing adjustment part. The shift gradient program creation part is configured to create a shift gradient program for causing the second dimension feeding device to execute shift gradient liquid feeding. The shift timing adjustment part is configured to adjust a shift timing to each stage in the shift gradient program created by the shift gradient program creation part based on a preliminary chromatogram acquired by the first dimension detector before the series of analysis operations for the sample to be analyzed.

MIXER SYSTEM FOR A LIQUID CHROMATOGRAPHY SYSTEM

A mixer system for a liquid chromatography system has a mixer block, a plurality of fluid inlets which are connected to a mixing chamber by a respective channel, and a fluid outlet for mixed fluid. Each channel has a valve assigned thereto which is adapted to control the fluid flow. An adapter which is attached to the mixer block and through which one of the channels passes is provided for each valve. The valve is attached to the appropriate adapter, and the valves are arranged in an annular shape around the mixer block.

Fluid mixer assembly

Disclosed is an assembly for mixing fluids (i.e., gases or liquids), and more particularly an assembly that accurately mixes two or more high-pressure fluid sources and is adapted for use in applications, such as for example, chromatography. The mixer assembly (100) includes, inter alia, a housing (10), an inlet fitting (40), and a mixer cartridge assembly (60). The housing (10) has a fluid receiving section (16) and a fluid discharge section (18) with an outlet (20) formed therein. A central bore (22) extends between the fluid receiving section (16) and fluid discharge section (18). An inlet fitting (40) is engaged with the housing (10) and has first (42) and second (44) fluid ports formed therein that extend from the fitting exterior to the fluid receiving section (16) of the housing (10). A mixer cartridge assembly (60) is disposed within the central bore (22) of the housing (10) and is positioned between the inlet fitting (40) and the downstream end portion of the housing (10). The ...

Synchronized vacuum degassing cycling in liquid chromatography

GENERATOR FOR ELECTRICALLY DIRECTIONS ELUENTIEN AND ASSOCIATED USE PROCEDURE

PRODUCTION PROCESS OF FILM AND COLUMN FOR CATION CHROMATOGRAPHY

LCMS technology and its uses

SAMPLE CONDITIONING SYSTEM FOR LOW PRESSURE GAS

A system and method for conditioning of very low pressure gas samples extracted from a source, heating the samples, boosting the pressure to a level appropriate for analysis, regulating the gas sample temperature and pressure to prevent dew-point dropout from Joules Thompson condensation, and passing the gas sample to an a remotely located analyzer or analyzer array where the electrical power for the pressurizing pump and heated regulator is provided by heat tracing.

Analyzing device

A liquid chromatography device (A) comprises a column (1) for holding a filler; an injection valve (4) capable of introducing a sample into the column (1) and capable of introducing a liquid mobile phase into the column (1); and mobile phase sending means (3) for sending the liquid mobile phase into the column (1) through the injection valve (4) from a mobile phase vessel (B) for housing the liquid mobile phase, wherein: a reservoir chamber (5) for temporarily holding the liquid mobile phase that has been sent from the mobile phase vessel (B) is provided between the mobile vessel (B) and the injection valve (4); and a fluid level detecting sensor for sensing the fluid level of the liquid mobile phase in the reservoir chamber (5) is provided. This structure enables the liquid to be supplied to the analysis without waste and without running out.

Automatic sample injector and liquid chromatograph

Device for recycling the carrier gas of a gas chromatograph

ION CHROMATOGRAPHY SYSTEMS WITH FLOW-DELAY ELUENT RECYCLE

IMMUNOCHROMATOGRAPHIC STRIP CAPABLE OF AVOIDING PROZONE EFFECT AND KIT COMPRISING SAME

The present invention, relating to an immunochromatographic strip comprising an adhesive plastic support body, a sample pad, a conjugate pad, a signal detection pad, and an absorption pad, provides: an immunochromatographic assay strip which further comprises an adjustment tape of a material not allowing a liquid sample to pass, being located between a sample input part of the sample pad and the conjugate pad, and which is capable of controlling the amount of a used analyte as the input sample moves to the conjugate pad along the length where the conjugate pad and the adjustment tape overlap; and a kit equipped with the strip.

Prefilled liquid cartridge for the supply of a sample separation device with an operating liquid

Prefilled liquid cartridge for fluidically connecting to a sample separation device for separating of components of a fluidic sample by using liquid of the liquid cartridge, wherein the liquid cartridge comprises a liquid container which is prefilled with liquid and a liquid removal access provided at the liquid container, adapted to be fluidically coupled with at least one liquid conduit of the sample separation device by only inserting the liquid cartridge in a corresponding liquid cartridge accommodation of the sample separation device.

PURGE METHOD FOR LOW PRESSURE GRADIENT FORMATION LIQUID CHROMATOGRAPHY

Described is a method for purging a fluid channel is a low pressure gradient formation liquid flow system. Control of the fluid channels for multiple solvents allows for one or more static volumes of solvents not intended for use in an isocratic flow to be purged from their fluid channels to avoid contamination of the isocratic solvent. Advantageously, the method avoids the need to modify equipment or to reconfigure a pumping system so that the inlet is directly coupled to a single solvent source. Thus there is no need to bypass existing valves and liquid coupling components where solvents are combined during conventional gradient operation. 1. A method for purging a fluid channel in a low pressure gradient formation liquid flow system , the method comprising:in a flow system having a cross connection, a first valve in communication with the cross connection through a first fluid channel, a second valve in communication with the cross connection through a second fluid channel, and a pump system in communication with the cross connection through a third fluid channel, the first fluid channel having a volume containing a first liquid and the second fluid channel having a volume containing a second liquid, drawing the first liquid from the first valve through the first fluid channel, the cross connection, the third fluid channel, and the pump system;closing the first valve to prevent flow between the cross connection and the first valve;opening the second valve to permit flow between the cross connection and the second valve;supplying the first liquid from the pump system through the third fluid channel, the cross connection, and the second fluid channel to at least the second valve to thereby replace the volume of the second liquid in the second fluid channel with the first liquid; andclosing the second valve to thereby prevent flow between the second valve and the cross connection.2. The method of wherein a volume of the first liquid that is supplied past the cross ...

ION CHROMATOGRAPHY SYSTEMS WITH FLOW-DELAY ELUENT RECYCLE

Chromatographic column

ЖХ-МС-ТЕХНОЛОГИЯ И ЕЕ ПРИМЕНЕНИЕ

... 1. Устройство для анализа образцов методами жидкостной хроматографии-масс-спектрометрии, содержащее насосное устройство, аналитическую колонку, устройство для ионизации электрораспылением и масс-спектрометр, где указанное насосное устройство сконструировано и смонтировано для подачи наноразмерного потока в аналитическую колонку, где указанная аналитическая колонка содержит стационарную фазу для жидкостной хроматографии и имеет внутренний диаметр менее чем 200 мкм, и где указанное устройство для ионизации электрораспылением содержит излучатель для электрораспыления, расположенный за аналитической колонкой по направлению потока образца и имеющий внутренний диаметр менее чем 70 мкм, а указанный масс-спектрометр расположен за излучателем, где указанное устройство для жидкостной хроматографии-масс-спектрометрии включает устройство для проведения по меньшей мере двумерной хроматографии, где хроматография в первом направлении включает хроматографию, проводимую по меньшей мере на сильном катионообменнике ...

High performance liquid chromatography unit, has eluent source for producing eluent stream in eluent line, where injection unit is connected to eluent line

The high performance liquid chromatography (HPLC) unit has an eluent source (1) for producing an eluent stream in an eluent line (2). An injection unit (3) is connected to the eluent line and a pillared unit (4) is connected to the injection unit. A detector (5) connected to the pillared unit. A mixing chamber and a particle filter in a common mixing chamber forming a mixing chamber unit (13) is arranged and is connected to streaming directly and consecutively. An independent claim is also included for a mixing chamber unit for use in a high performance liquid chromatography unit.

Fractional purification apparatus

Mixed liquid channel (31) is connected to trapping channel (22) upstream of trap column (23). A mixed liquid of diluent and additive suctioned/emitted by respective liquid feed pumps (35,36) is fed to the mixed liquid channel (31). At the flow of eluate containing a target compound through the trap column (23), the eluate is diluted with the diluent, so that the elution power is lowered to thereby ease trapping of the target compound in the trap column (23). On the other hand, although lowering of the solubility of mobile phase in the eluate would ease precipitation of not only the target compound but also foreign matter, the precipitation can be inhibited by the action of the additive to thereby enable prevention of clogging of the trap column (23) and piping.

Preparative separation/ purification system

Ion mobility spectrometer with GC column and regulated gas cycle

VENTIL UND VERWENDUNG DES VENTILS

The invention relates to a valve, in particular a sample injection valve, for a device (1) for synthesizing, analyzing, and/or separating, comprising at least three liquid connections (3', 4', 5', 6', 7', 7"), a housing (8) as a valve part, and a valve body (9) as another valve part for selectively connecting the liquid connections (3', 4', 5', 6', 7', 7") by means of at least one flow channel (10, 11, 12) bounded at least partially by sealing surfaces (10', 11', 12') between the housing (8) and the valve body (9), wherein the housing (8) and/or the valve body (9) are supported in such a way as to be movable relative to each other. In order to a create a one-way valve, at least one valve part (8 or 9) adjacent to the sealing surface (10', 11', 12') and made of a plastic material can be plastically deformed according to a relative position (9'), in particular of the valve body (9), in order to be able to withstand the elevated pressure loads in the flow channel (10, 11, 12) in a liquid-tight ...

Liquid chromatograph and liquid feeder for liquid chromatograph

A liquid chromatograph operated in the gradient mode is provided in which it is possible to accelerate the mixing of a plurality of solvents without necessitating an increase in channel volume. The liquid chromatograph includes a liquid feeder that draws a plurality of solvents out of the containers and sends the solvents to a sample injector, a separation column that separates a sample into components, and a detector that detects the components of the sample which are sent from the separation column. The liquid feeder draws the plurality of solvents through an intake port and sends the solvents through a discharge port to the sample injector, and the liquid feeder, simultaneously therewith, mixes the plurality of solvents while the solvents are flowing from the intake port to the discharge port. The liquid feeder is equipped with a cylinder and a plunger that reciprocates within the cylinder, the inner wall of the cylinder having, formed therein, recesses that cause the plurality of solvents ...

Prozone effect-preventable immunochromatographic strip and a kit comprising the same

The present invention relates to an immunochromatography analysis strip and a kit containing the strip. The immunochromatography analysis strip and the kit containing the strip comprise: a) an adhesive plastic support member; b) a sample pad arranged on the upper surface of the adhesive plastic support member from an upper region to a lower region and equipped with a buffer inlet part in the upper region and a sample inlet part in the lower region to receive a liquid sample to be analyzed; c) a conjugate pad, for containing a conjugate to be specifically bonded to the analytes contained in the liquid sample of the sample pad, which is placed in a lower direction of the lower region of the sample pad; d) a signal detection pad, placed adjacent to the conjugate pad in the lower end of the conjugate pad, which contains a signal detection part for detecting analytes in the liquid sample and a control part to confirm the transfer of the liquid sample to the absorbing pad; and e) an immunochromatographic strip containing the absorbing pad placed adjacent to the signal detection pad in the lower end of the signal detection pad; and further comprises a control tape placed between the sample inlet part of the sample pad and the conjugate pad based on a material which prevents the pass of the liquid sample. Furthermore, the immunochromatography analysis strip and the kit containing the strip are provided to control the amount of analytes for analysis after transferring the sample injected according to the overlapped length of the conjugate pad and the control tape to the conjugate pad.

Preparative separation/purification system

After a target compound is captured by an adsorbent within a trap column (7) held by a column rack (8) in a substantially vertical position and the column is filled with water to wash its inside, dichloromethane is slowly introduced from an inlet end (7a) on the lower side by a pump (5). The water within the trap column (7) is pushed upward by dichloromethane and exits from an outlet end (7b), to be disposed of via a two-way selector valve (12) and a disposal passage (14). Dichloromethane elutes the captured target compound. Therefore, when the eluate, which initially consists of water, changes via an emulsion to dichloromethane, the eluate contains an adequately high concentration of target compound. At this point, the two-way valve (12) is switched to a preparative separation passage (13). As a result, a solution that is adequately free from water and yet contains the target compound can be collected into a collection container (17), and the target compound in solid forms can be quickly ...

Multi-measurement flow cell assembly for liquid chromatography

A detector for detecting constituents of a liquid for use in liquid chromatography is disclosed. The detector includes a first optical flow cell body and a second optical flow cell body, each having a channel therethrough that allows passage of a liquid from an inlet port to an outlet port. The first and second optical flow cell bodies are arranged in series such that the liquid exiting the outlet port of the first optical flow cell body enters the inlet port of the second optical flow cell body. An insulator resides between the first optical flow cell body and the second optical flow cell body, which is adapted to electrically insulate the first optical flow cell body from the second optical flow cell body while allowing the liquid to pass from the first optical flow cell body to the second optical flow cell body. The first optical flow cell body is adapted to facilitate measurement of absorption by the liquid of a first wavelength of light, and second optical flow cell body is adapted ...

Evacuable inlet for gas chromatograph injector

A gas chromatography (GC) system comprises: a sample injector adapted to receive a liquid sample into an interior cavity thereof and to volatilize the liquid sample; a GC column configured to receive the volatilized sample from the sample injector; a carrier gas inlet line fluidically coupled to a gas inlet port of the sample injector; a septum purge vent line fluidically coupled to a first gas outlet port of the sample injector; a split-flow vent line fluidically coupled to a second gas outlet port of the sample injector; and a vacuum system configured to apply vacuum to the septum purge vent line, the split-flow vent line and the interior cavity of the sample injector. Alternatively, the vacuum system may be coupled to a vacuum port of the sample injector. A separate flow of helium gas may be supplied to an inlet of the GC column.

CHROMATOGRAPHI COLUMN

ELECTROLYTIC GENERATOR FOR THE PRODUCTION OF ELUIERUNGSMITTEL AND ITS USE

Circulatory system for mobile phase of high performance liquid chromatograph and control method of circulatory system

ANALYSIS DEVICE AND METHOD THEREOF FOR PERFORMING THE WRITING OF XYLENES USING A SIMULATED MOVING BED RAMAN SPECTROMETER

La présente invention décrit un dispositif et une méthode d'analyse associée à ce dispositif, ledit dispositif comprenant une cellule d'analyse (8) contenant une optrode (10), recevant un signal laser d'entrée et envoyant un signal de sortie vers le spectromètre Raman, et ladite méthode utilisant deux voies d'analyse; une voie d'analyse 1 connectée au courant de circulation interne allant de l'adsorbeur B à l'adsorbeur A, et une voie d'analyse 2 connectée au raffinat distillé issu de la colonne de distillation C.

aparelho e método para remover gás antes de detecção e/ou da análise de amostra

METHOD FOR MEASURING 2-AMINO-3-[2-(Α- MANNOPYRANOSYL)INDOL-3-YL]PROPIONIC ACID IN SAMPLE

Provided is a method for measuring 2-amino-3-[2-(α-mannopyranosyl)indol-3-yl]propionic acid (referred to as "Trp-Man", hereinbelow) in a sample accurately by reverse-phase high performance liquid chromatography (referred to as "reverse-phase HPLC", hereinbelow). A method for measuring Trp-Man in a sample by reverse-phase HPLC, said method being characterized in that a mobile phase to be used is one containing a sulfonic acid-type ion-pair reagent. The method for measuring Trp-Man by reverse-phase HPLC according to the present invention is effective for the diagnosis of a kidney function and the like.

Ion mobility spectrometer with GC column and internal controlled gas circulation

An ion mobility spectrometer with a GC column and an internal circulation system is provided which can be used in trace gas analysis. Due to the special design of the gas circulation, the parameters: carrier gas velocity in the GC column, the flow rate of the gas to be analyzed and the flow rate of the drift gas can be varied extensively independently and without reaction. Additional pumps and gas splitters are arranged in the circulation system for this purpose.

Protein chromatography system

The invention features an apparatus for the separation and analysis of proteins, which includes a sample input, a first liquid chromatography column, a multiport injection valve connecting the sample input to the column, a pump for providing variable pressure delivery of a solution to the column via the multiport valve, and a program for specifying a sequence of system control programs.

PREPARATIVE SEPARATION/PURIFICATION SYSTEM

A mixed liquid passage (31) is connected to a trapping passage (22) at a point upstream of a trap column (23). A mixture of a diluting liquid and an additive agent, which are respectively drawn and sent by pumps (35) and (36), is supplied into the mixed liquid passage (31). When an eluate containing a target compound is passed through the trap column (23), the eluate is diluted with the diluting liquid and hence its elution capability decreases, so that the target compound can be easily captured in the trap column (23). Meanwhile, the solubility of the mobile phase in the eluate decreases, causing not only the target component but also foreign compounds to more easily precipitate. However, their precipitation is prevented by the effect of the additive agent. Thus, clogging of the trap column (23) or pipes is prevented.

LCMS technology and its uses

HYBRID SUPERCRITICAL FLUID CHROMATOGRAPHY METHOD

CHROMATOGRAPHIC PURIFICATION METHOD

A chromatographic purification method for the isolation of a desired product fraction from a mixture using 2 chromatographic columns, comprises, within one cycle to be carried out at least once, the following steps: a first batch step, wherein said columns are disconnected and a first column is loaded with feed and its outlet is directed to waste, and from a second column desired product is recovered and subsequently the second column is regenerated; a first interconnected step, wherein the outlet of the first column is connected to the inlet of the second column, the first column is loaded beyond its dynamic breakthrough capacity with feed, and the outlet of the second column is directed to waste, a second batch step analogous to the first batch step but with exchanged columns; and a second interconnected step, analogous to the first interconnected step but with exchanged columns.

Liquid chromatograph

A liquid chromatograph has a mobile phase delivery section including, between mobile phase channels connected respectively to a plurality of mobile phase containers and a delivery pump, channel switching valves for selecting one of the mobile phase channels. This liquid chromatograph whose channel switching valves have a hierarchical structure including at least two stages includes a valve connection state setting section that is configured to set the connection state between the channel switching valves and the connection state between the channel switching valves and the mobile phase channels, and further includes a per channel total delivery amount calculation section that is configured to calculate the total delivery amount of mobile phase per mobile phase channel based on a connection state according to the valve connection state setting section, the delivery amount per unit time by the delivery pump, and the operation time of the delivery pump.

GAS CHROMATOGRAPH

When a flow rate of a carrier gas calculated by a column flow rate calculation unit is controlled to be a first flow rate, and when the flow rate of the carrier gas is controlled to be a second flow rate, the flow rates of the carrier gas are detected by a whole flow rate detection unit, and a gas leakage determination unit determines of leakage of the carrier gas on the basis of the rates. Since the flow rate of the carrier gas is changed and the detected value of the whole flow rate detection unit is used at different flow rates, a case can be discriminated where an offset occurs in the detected value of the whole flow rate detection unit and a case where leakage of the carrier gas occurs. Thus, it is satisfactorily determined the presence or absence of the leakage of the carrier gas. 1. A gas chromatograph comprising:a column into which a sample is introduced from a column inlet;a sample introduction unit which introduces the sample together with a carrier gas into the column from the column inlet;a gas supply unit which communicates with the sample introduction unit to supply a carrier gas to the sample introduction unit;a whole flow rate detection unit which detects a flow rate of the carrier gas supplied from the gas supply unit;a pressure detection unit which detects a pressure of the carrier gas at the column inlet;a column flow rate calculation unit which calculates a flow rate of the carrier gas passing through the column, on the basis of the pressure of the carrier gas at the column inlet;a first flow rate control unit which controls the flow rate of the carrier gas supplied from the gas supply unit so that the flow rate of the carrier gas calculated by the column flow rate calculation unit becomes a first flow rate;a second flow rate control unit which controls the flow rate of the carrier gas supplied from the gas supply unit so that the flow rate of the carrier gas calculated by the column flow rate calculation unit becomes a second flow rate different ...

Detector for liquid chromatograph

A detector for a liquid chromatograph, includes a detector that detects components in liquid, a pipe that guides liquid to the detector, a casing that contains at least part of the pipe and the detector and has a bottom portion, and a thermal insulator provided on the bottom portion in the casing, wherein a discharge port is provided in the bottom portion of the casing, the thermal insulator has an upper surface and a lower surface, a first opening is provided in the upper surface of the thermal insulator, and a second opening is provided in the lower surface of the thermal insulator to overlap with the discharge port in a plan view, a flow path that guides liquid from the first opening to the second opening is provided in the thermal insulator, and the flow path has a bend portion.

SAMPLE INTRODUCTION DEVICE

A switching mechanism 110 can perform switching to a pressurized state in which gas is supplied from a pipe 203 to an insertion tube 101, or a derivation state in which gas in a head space 23 that is pressurized is derived from the insertion tube 101 to the pipe 207 via a collection unit 104. The switching mechanism 110 includes a discharge valve 103 that puts the insertion tube 101 and the pipe 207 into a non-communication state in the pressurized state and puts the insertion tube 101 and the pipe 207 into a communication state in the derivation state. A resistance pipe 206 supplies gas to a channel between the collection unit 104 and the discharge valve 103 in the derivation state.

УСТРОЙСТВО И СПОСОБ УДАЛЕНИЯ ГАЗА ПЕРЕД ДЕТЕКТИРОВАНИЕМ И/ИЛИ АНАЛИЗОМ ПРОБЫ

... 1. Система детектирования пробы, включающая а. водную мобильную фазу, содержащую газ; b. камеру, имеющую вход и выход, в которой вход принимает, по крайней мере, часть мобильной фазы; с. поглотитель, размещенный в камере, эффективный для уменьшения концентрации газа внутри мобильной фазы, когда мобильная фаза перемещается от входа к выходу; и d. детектор. 2. Система детектирования пробы по п.1, в которой система выбрана из группы, состоящей из ионной хроматографии, жидкостной хроматографии, ультрафиолетового детектирования, измерения коэффициента преломления, флюоресценции, хемилюминесценции, и масс спектроскопии. 3. Система детектирования пробы по п.1, в которой выход камеры через текучую среду связан с входом детектора. 4. Система детектирования пробы по п.1, в которой газ выбран из кислорода, двуокиси углерода, оксида углерода, азота, водорода, муравьиной кислоты и трифторуксусной кислоты. 5. Система детектирования пробы по п.1, в которой газ представляет собой двуокись углерода. 6.

СИСТЕМА ПОДГОТОВКИ ПРОБЫ ДЛЯ ГАЗА НИЗКОГО ДАВЛЕНИЯ

Ion mobility spectrometer with GC column and internal regulated gas cycle

Sample Fragmentation Device

Gas purification apparatus and trace substance detection device

Using multi-component test sample for determining real parameter value of sample separation

A process which, on the basis of a provided test sample 100 comprising a mix of a plurality of pre-known sample components 102 and on the basis of provided absolute sample separation properties 104 for each of the sample components,comprises experimentally determining a real sample separation result 106 by executing a sample separation method for separating the test sample by a sample separation apparatus 10, and determining a real value 108 of at least one operation parameter based on a comparison between the absolute sample separation properties and the real sample separation result for characterizing a real course of the sample separation method. The determined real value may be used as a performance indicator in liquid chromatography. Preferably, at least part of the sample components have properties with pronounced temperature dependence, at least part of the sample components have properties with pronounced flowrate dependence, and at least part of the sample components have properties with pronounced dependence on solvent composition, in particular a gradient profile.

Solvent channel identification

A solvent supply system (210) for supplying solvent for one or more chromatographic separation systems (10) each comprises one or more input channels (250). Each input channel (250) is configured for fluidically coupling with a respective solvent container (240) for supplying the respective input channel (250) with a respective solvent from respective solvent container (240). The solvent supply system (210) comprises a solvent identification unit (260) configured for providing a channel identification (270) for identifying a specific solvent container (240) to supply a specific input channel (250) of a specific chromatographic separation system (10), and for assigning the channel identification (270) to the specific solvent container (240). The solvent supply system provides improved handling of solvent containers and reduces mistakes connecting containers to a specific chromatography system.

SUPERCRITICAL FLUID CHROMATOGRAPHY METHOD FOR HYBRID

L'invention concerne un procédé de chromatographie en phase supercritique ou liquide d'un produit P. Le procédé selon l'invention comprend en continu et dans l'ordre : - une étape analytique (10, 30) selon une voie analytique (1) ; - une étape d'aiguillage (X1, X2) comprenant une opération de changement de voie (402, 502) entre la voie analytique (1) et une voie préparative (2) ; - en cas de sélection de la voie préparative (2) à l'étape d'aiguillage, une étape préparative (20) selon la voie préparative (2). The invention relates to a method for chromatography in the supercritical or liquid phase of a product P. The process according to the invention comprises continuously and in sequence: - an analytical step (10, 30) according to an analytical route (1) ; a switching step (X1, X2) comprising a lane change operation (402, 502) between the analytical channel (1) and a preparative channel (2); - in case of selection of the preparative route (2) in the switching step, a preparative step (20) according to the preparative route (2).

Column filter retainer connector with structural reinforcement and biocompatible fluid passageway

A connector with a biocompatible fluid passageway to be used as a column filter retainer and the method for manufacturing it is described. The connector has a reinforcement insert and a biocompatible molding covering portions of the reinforcement insert. The reinforcement insert has a first portion, a second portion, and a middle portion between the first portion and the second portion. The first and second portions have threaded sections and each have a plurality of non-threaded sections. For a given portion, the junction of the non-threaded sections forms a lip by which to prevent the molded material from flowing into the threaded sections. In certain embodiments, an interior web is used in the reinforcement insert to provide additional structural support.

Bubble reduction device, chromotography device, bubble reduction method, and bubble reduction program

A bubble reduction device, chromatography device, bubble reduction method and bubble reduction program capable of reducing bubbles in an eluent. Included are a liquid accommodation portion (12A), a liquid supply apparatus (63), an air layer formation apparatus (72), a first channel (14) and an evacuation portion (76). The liquid accommodation portion (12A) accommodates a liquid that is to elute an analysis component from a specimen adsorbed to an adsorption portion. The liquid supply apparatus (63), by operation of a rod (66) pushing up and pulling down, sucks and discharges the liquid through an aperture portion of a tube portion (65), the aperture portion being oriented upward. The air layer formation apparatus (72) forms an air layer in the tube portion (65). The first channel (14) connects the liquid supply apparatus (63) with the liquid accommodation portion (12A). The evacuation portion (76) is connected to the first channel (14) via a first switching valve (74) and evacuates the ...

Fluidmischanordnung und chromatographisches System

Mischanordnung, umfassend: a) ein Gehäuse mit gegenüberliegenden Endabschnitten stromaufwärts und stromabwärts, wobei der stromaufwärts gelegene Endabschnitt einen Fluidaufnahmebereich umfasst und der stromabwärts gelegene Endabschnitt einen Fluidabgabebereich umfasst, der einen darin ausgebildeten Fluidauslass aufweist, wobei das Gehäuse der Mischanordnung eine zentrale Bohrung definiert, die sich axial zwischen dem Fluidaufnahmebereich und dem Fluidabgabebereich erstreckt; b) eine Einlassfassung, die mit dem stromaufwärts gelegenen Endabschnitt des Gehäuses in Eingriffnahme steht und darin ausgebildete erste und zweite Fluideinlassanschlüsse aufweist, die sich von der Außenseite der Fassung zu dem Fluidaufnahmebereich des Gehäuses erstrecken; und c) eine Mischkartuschenanordnung, die innerhalb der zentralen Bohrung des Gehäuses angeordnet ist und zwischen der Einlassfassung und dem stromabwärts gelegenen Endabschnitt des Gehäuses positioniert ist, wobei die Mischkartuschenanordnung umfasst ...

Device for fragmenting sample

A device for fragmenting a sample, said device comprising a liquid feeding pump 2, a sample injection section 3 and a separation column 6 that are connected to each other by a pipeline for speeding up the fragmentation of the sample such as a protein or peptide and improving the introduction efficiency thereof into a detector such as a mass spectrometer. The device further comprises: a heating section 4 that heats the pipeline between the sample injection section 3 and the separation column 6; and a pressure regulating section 5 that is disposed between the heating section 4 and the separation column 6 and regulates the internal pressure in the pipeline heated by the heating section 4.

Synchronized vacuum degassing cycling in liquid chromatography

A method for vacuum degassing of a liquid such as a solvent for a liquid chromatography system comprising: modulating application of a vacuum to a fluid channel of a degasser so that each volume of a liquid drawn from the degasser experiences a residence time that is equal to the residence times of the other volumes. The residence time is determined as a time that the volume resides in the fluid channel under application of the vacuum and to a magnitude of the applied vacuum. Also claimed is an apparatus comprising: a degasser 24 having fluid channel (44) to conduct a liquid; a vacuum source in communication with degasser 24 that applies vacuum to fluid channel (44); and a processor 14 that generates a control signal to modulate the application of the vacuum to fluid channel (44) of degasser 24. The method and apparatus is preferably applied to low pressure gradient formation where two or more liquids are combined into a single flow, such as a gradient mobile phase.

Synchronized vacuum degassing for liquid chromatography

ELECTROLYTIC ELUENT GENERATOR AND METHOD OF USE

DIRECTED-FLOW ASSAY DEVICE

A diagnostic assay device that directs an applied sample to the analytical membrane of a directed flow device. The device has a sample receiving port defined by layers of built-up material on one end of the test strip. The port contains the sample and specifically directs it to the membrane in a controlled fashion. Additional features include configuration of the housing in a general C-shape with the test strip spanning the opening of the C-shape to allow access by a reader device. See Figure 5. A preferred method employs superparamagnetic particles to label the target analytes for detection and measurement by means of an electromagnetic reader device.

LCMS TECHNOLOGY AND ITS USES

The present invention relates to an improved LCMS technology and its uses in methods for the selective identifica-tion and characterization of immunogenic pathogen associated epitopes, and the use thereof in vaccine development. One way of bridging the knowledge gap on T cell epitopes is to apply a new platform technology, "immunoproteomics", to directly assess the epitope display at the surface of antigen presenting cells by nanoscale mass spectrometry of extracted peptide samples. This is the only methodology that can provide unbiased insight into epitope features such as the exact molecular nature, diversity, abundance, dynamics and PTM of T cell epitopes originating from pathogen-derived proteins. Therefore, this platform technology and im-munoproteomics should become an intrinsic part of vaccinology.

Liquid chromatograph and liquid amount detection method

LIQUID CHROMATOGRAPHY FILLER, LIQUID CHROMATOGRAPHY COLUMN AND METHOD FOR ANALYZING AMINE COMPOUND

LIQUID CHROMATOGRAPHY DEVICE AND LIQUID CHROMATOGRAPHY

액체 크로마토그래피 장치(A)는, 시료 제작 수단(1)과, 시료의 성분을 분리하는 칼럼(52)과, 용리액(La), (Lb)을 칼럼(52)에 공급하는 송출수단(3)을 포함하는 용리액 송출수단과, 칼럼(52)에 일정량의 상기 시료와 용리액(La), (Lb)을 도입가능한 유로 전환 밸브(2)와, 칼럼(52)에 의해서 분리된 상기 시료의 성분과 상기 용리액(La)으로 이루어진 피검액의 분석을 행하는 분석수단과, 제어수단(6)을 포함하고, 상기 용리액 공급수단은 용리액(La), (Lb)을 비혼합 상태에서 유로 전환 밸브(2)에 공급한다. 이러한 구성에 의해, 분석 시간의 단축 및 용리액 소비의 감소를 도모하는 것이 가능하다. The liquid chromatography apparatus (A) includes a sample preparation means (1), a column (52) for separating the components of the sample, and a delivery means (3) for supplying the eluent (La) and (Lb) to the column (52). Eluent dispensing means comprising: a flow path switching valve (2) into which a predetermined amount of the sample and the eluent (La), (Lb) can be introduced into the column (52), and the component of the sample separated by the column (52); Analysis means for analyzing the test liquid consisting of the eluent La and the control means 6, wherein the eluent supply means flow path switching valve (2) in the non-mixed state of the eluent (La), (Lb) To feed. With this configuration, it is possible to shorten the analysis time and reduce the eluent consumption.

Gas-liquid separator for chromatography systems

Purge method for low pressure gradient formation liquid chromatography

Described is a method for purging a fluid channel is a low pressure gradient formation liquid flow system. Control of the fluid channels for multiple solvents allows for one or more static volumes of solvents not intended for use in an isocratic flow to be purged from their fluid channels to avoid contamination of the isocratic solvent. Advantageously, the method avoids the need to modify equipment or to reconfigure a pumping system so that the inlet is directly coupled to a single solvent source. Thus there is no need to bypass existing valves and liquid coupling components where solvents are combined during conventional gradient operation.

Vorbefüllte Flüssigkeitskartusche zur Versorgung eines Probentrenngeräts mit einer Betriebsflüssigkeit

Vorbefüllte Flüssigkeitskartusche (80) zum fluidischen Anschließen an ein Probentrenngerät zum Trennen von Komponenten einer fluidischen Probe unter Verwendung von Flüssigkeit (402) der Flüssigkeitskartusche (80), wobei die Flüssigkeitskartusche (80) einen Flüssigkeitsbehälter (404), der mit Flüssigkeit (402) vorbefüllt ist, und einen an dem Flüssigkeitsbehälter (404) vorgesehenen Flüssigkeitsentnahmeanschluss (406) aufweist, der eingerichtet ist, mittels bloßen Einführens der Flüssigkeitskartusche (80) in eine korrespondierende Flüssigkeitskartuschenaufnahme (408) des Probentrenngeräts mit mindestens einer Flüssigkeitsleitung (410) des Probentrenngeräts fluidisch gekoppelt zu werden.

Schaltventil für die Flüssigkeitschromatographie, insbesondere Hochdruck-Schaltventil für die Hochleistungsflüssigkeitschromatographie

Die Erfindung betrifft ein Schaltventil für die Flüssigkeitschromatographie, insbesondere Hochdruck-Schaltventil für die Hochleistungsflüssigkeitschromatographie, mit einem in einem Gehäuse (3) angeordneten Stator (29), welcher mehrere Anschlussports (31) aufweist, und mit einem im Gehäuse (3) angeordneten, drehbaren Rotor (23), der in vorbestimmten, durch zugeordnete Winkelstellungen definierten Schaltstellungen mit dem Stator (29) zur fluidischen Verbindung oder Trennung von vorbestimmten Anschlussports (31) zusammenwirkt, wobei der Rotor (23) mittels einer im Gehäuse (3) angeordneten Lager- und Anpresseinrichtung (8) drehbar gelagert und mit einer vorgegebenen Anpresskraft in Richtung auf den Stator (29) beaufschlagt ist. Erfindungsgemäß weist die Lager- und Anpresseinrichtung (8) ein Ausgleichselement (21, 21) auf, welches den Rotor zur Übertragung der Anpresskraft beaufschlagt, wobei die Längsachse des Ausgleichselements (21, 21) im Wesentlichen mit der Drehachse des Rotors (23) fluchtet ...

Gasreinigungseinrichtung und Spurensubstanzdetektionsvorrichtung

Die vorliegende Offenbarung bezieht sich auf eine Gasreinigungseinrichtung und eine Spurensubstanzdetektionsvorrichtung. Die Gasreinigungseinrichtung umfasst eine erste Reinigungskomponente (1), eine zweite Reinigungskomponente (5) und eine Schaltkomponente, wobei die Schaltkomponente zwischen einem ersten Zustand und einem zweiten Zustand umgeschaltet werden kann, die erste Reinigungskomponente (1) und eine zu reinigende Komponente eine Gasreinigungsschleife im ersten Zustand bilden, und die zweite Reinigungskomponente (5) ein Regenerationsgas für die erste Reinigungskomponente (1) im zweiten Zustand liefern kann, so dass Wasserdampf und Verunreinigungen in der ersten Reinigungskomponente (1) nach außen abgeführt werden. In der Gasreinigungseinrichtung wird die gefilterte Luft als Regenerationsgas verwendet, um eine Sekundärverschmutzung im Recyclingprozess des Reinigungsmittels zu verhindern; ferner kann mittels der Zustandsumschaltfunktion der Schaltkomponente die gegenseitige Störung ...

Mobile phase supply device with fluidically normally closed port and cap devices

A mobile phase supply device 100 for supplying a mobile phase 102 (Fig. 2) for a sample separation/chromoatography apparatus 10 (Fig. 1) for separating a fluidic sample 92 (Fig. 1), wherein the mobile phase supply device 100 comprises a fluidically normally closed cap device 106 mounted or configured to be mounted on a mobile phase container 104 containing a mobile phase 102, and a fluidically normally closed port device 108 configured for being mechanically connected with the cap device 106 in such a way that, upon establishing a mechanical connection between the port device 108 and the cap device 106, both the port device 108 and the cap device 106 are converted into a fluidically opened configuration. Further disclosed is a method of using the cap 106 and port device 108 in a mobile phase supply device 100.

Method and apparatus for generating a salt solution for liquid chromatography

GAS PURIFICATION APPARATUS AND TRACE SUBSTANCE DETECTION DEVICE

The present disclosure relates to a gas purification apparatus and a trace substance detection device. The gas purification apparatus includes a first purification component (1), a second purification component (5) and a switching component, wherein the switching component can be switched between a first state and a second state, the first purification component (1) and a component to be purified form a gas purification loop in the first state, and the second purification component (5) can provide a regeneration gas for the first purification component (1) in the second state, so that water vapor and impurities in the first purification component (1) are discharged to outside. In the gas purification apparatus, the filtered air is used as the regeneration gas to prevent secondary pollution in a recycling process of the purificant; furthermore, by means of the state switching function of the switching component, the mutual interference between the two working states of purification and regeneration ...

ATMOSPHERIC MONITORING SYSTEM AND METHOD USING SENSOR AND GAS CHROMATOGRAPHY

The present invention relates to an atmospheric monitoring system and method using a sensor and gas chromatography comprising: a first suction portion for sucking air through a sample line by means of a first vacuum pump; a sensor room disposed on the sample line and having a plurality of sensors arranged to measure each of a plurality of substances contained in the air sucked through the sample line; a branch line branched from a front end of the sensor room on the sample line and provided with an opening and closing valve; and a GC analysis unit for performing a gas chromatography (GC) analysis on the air supplied through the branch line due to the opening of the opening and closing valve. According to the present invention, it is possible to increase accuracy by analyzing a substance measured above the reference value concentration among odor substances monitored in real time by GC, and thus, increase the accuracy and reliability of odor monitoring by preventing interference of different ...

Schaltventil, insbesondere Hochdruck-Schaltventil für die Hochleistungsflüssigkeitschromatographie

Die Erfindung betrifft ein Schaltventil (200, 500), insbesondere Hochdruck-Schaltventil für die Hochleistungsflüssigkeitschromatographie, mit einem in einem Gehäuse (260) angeordneten Stator (202), welcher mehrere Anschlussports aufweist, und mit einem im Gehäuse (260) angeordneten, drehbaren Rotor (210), der in vorbestimmten, durch zugeordnete Winkelstellungen definierten Schaltstellungen mit dem Stator (202) zur fluidischen Verbindung oder Trennung von vorbestimmten Anschlussports zusammenwirkt, wobei der Rotor (210) mittels einer im Gehäuse (260) angeordneten Lager- und Anpresseinrichtung (230) drehbar gelagert und mit einer vorgegebenen Anpresskraft in Richtung auf den Stator (202) beaufschlagt ist. Erfindungsgemäß weist die Lager- und Anpresseinrichtung (230) eine Federeinrichtung (230) auf, welche den Rotor (210) oder ein damit unmittelbar oder mittelbar verbundenes Element (220) zur Übertragung der Anpresskraft beaufschlagt, und welche den Rotor (210) oder den Rotor (210) und das ...

ANALYSIS METHOD AND ANALYSIS DEVICE FOR SUBSTANCE TO BE MEASURED

An analysis method for a substance to be measured, including (a) introducing a sample into a flow channel having a bypass flow channel and introducing the sample introduced into the bypass flow channel into a measuring device so as to measure signal intensity or concentration of a substance to be measured and/or a carrier in the sample; (b) introducing a sample that has not been introduced into the bypass flow channel into a column so as to adsorb the substance to be measured and/or the carrier; and (c) introducing an eluate into the column, eluting the substance to be measured and/or the carrier adsorbed on the column, and introducing the substance and/or the carrier into the measuring device so as to measure the signal intensity or concentration of the eluted substance to be measured and/or the carrier.

Liquid chromatograph and liquid chromatograph analysis method

Carrier for affinity chromatography

저압 가스용 샘플 컨디셔닝 시스템

... 소스로부터 추출된 매우 낮은 압력의 가스 샘플을 컨디셔닝하는 시스템 및 방법은, 샘플을 가열하고, 분석을 위해 적절한 레벨로 압력을 승압하고, 줄-심프톤 응축으로부터 이슬점 강하를 방지하기 위해 가스 샘플의 온도 및 압력을 조절하고, 가스 샘플을 원격으로 위치된 분석기 또는 분석기 어레이로 통과시키는데, 가압 펌프 및 가열 조절기를 위한 전력은 히트 트레이싱에 의해 제공된다.

AROMATIC POLYSULFONE AND AROMATIC POLYSULFONE COMPOSITION

The invention provides an aromatic polysulfone including an aromatic polysulfone having at least one highly polar functional group at its terminus, wherein in the aromatic polysulfone, an area of a signal attributed to the aromatic polysulfone having a highly polar functional group with respect to a total area of all signals attributed to the aromatic polysulfone in a chromatogram obtained by measurement through a gel permeation chromatography method under the following conditions is 19% or more and 100% or less, wherein sample injection volume is 5 μL, column is “Shodex KF-803” manufactured by Showa Denko K.K., column temperature is 40° C., eluent is N,N-dimethylformamide, eluent flow rate is 0.5 mL/min, and detector is ultraviolet-visible spectrophotometer (UV).

Fluid Switching Valve and Liquid Chromatograph Apparatus Using the Same

Provided are: a flow path switching valve that reduces the pressure load in a contact surface outer peripheral section of the flow path switching valve and inhibits friction between constituent components; and a liquid chromatographic device using the flow path switching valve. The flow path switching valve is provided with a stator having a plurality of through holes and a seal having conduction grooves for causing the through holes to conduct. The seal has a first portion present vertically beneath a region comprising at least a surface of contact with the stator, and a second portion having lower rigidity than the first portion, on the outside of the first portion. Due to this configuration, it is possible to reduce the pressure load when a flow path of a liquid is switched under high-pressure conditions, and inhibit the phenomenon of friction itself. 1. A fluid switching valve comprising:a stator having a plurality of through holes; anda seal having a connection groove through which the through holes are connected to each other,wherein the seal includesa first portion that is present vertically downward with respect to a region containing at least a contact surf ace with the stator, anda second portion that is provided outside the first portion and has lower rigidity than the first portion.2. The fluid switching valve according to claim 1 ,wherein the second portion is formed by providing a groove on a surface of the seal other than a surface opposite to the stator.3. The fluid switching valve according to claim 2 ,wherein an inner diameter of the groove provided in the seal is equal to or greater than a diameter of the connection groove of the seal and is equal to or less than a diameter of the contact surface between the seal and the stator.4. The fluid switching valve according to claim 1 ,wherein in the second portion, a space is provided in a region of the surface of the seal opposite to the stator excluding a region contacting the stator.5. The fluid ...

Sample Conditioning System for Low Pressure Gas

A system and method for conditioning of very low pressure gas samples extracted from a source, heating the samples, boosting the pressure to a level appropriate for analysis, regulating the gas sample temperature and pressure to prevent dew-point dropout from Joules Thompson condensation, and passing the gas sample to an a remotely located analyzer or analyzer array where the electrical power for the pressurizing pump and heated regulator is provided by heat tracing.

Schaltventil für die Flüssigkeitschromatographie, insbesondere Hochdruck-Schaltventil für die Hochleistungsflüssigkeitschromatographie

Schaltventil für die Flüssigkeitschromatographie, insbesondere Hochdruck-Schaltventil für die Hochleistungsflüssigkeitschromatographie, (a) mit einem Stator (29), in welchem mehrere Ports ausgebildet sind, wobei jeder Port durch jeweils einen Kanal gebildet ist, der an einem Ende mit jeweils einem Anschlussport (31) verbunden ist und der am anderen Ende an einer Statorstirnfläche des Stators (29) einen vorbestimmten Port-Öffnungsquerschnitt aufweist, (b) mit einem Rotor (23), welcher eine mit der Statorstirnfläche zusammenwirkende Rotorstirnfläche aufweist, in welcher zumindest eine oder mehrere Nuten ausgebildet sind, die abhängig von der Drehposition des Rotors gegenüber dem Stator in zumindest einer vorbestimmten Schaltstellung jeweils vorbestimmte Port-Öffnungsquerschnitte druckdicht verbinden, (c) mit einer Antriebseinrichtung (45, 37) zum rotatorischen Antrieb des Rotors (23), (d) mit einer Einrichtung (53) zur Erfassung der rotatorischen Position des Rotors (23), welche ein der absoluten ...

Method for cleaning flowpath components of fluid flowpath of analysis device e.g. liquid chromatography apparatus, involves pressurizing flowpath components of fluid flowpath of analysis device with liquid carbon dioxide

The method (100) involves pressurizing (102) flowpath components (206,208,210,212,214,215,217) of a fluid flowpath (202) of an analysis device (200) with liquid carbon dioxide. The flowpath components are purged (104) with a solvent based on pressurization process. The flowpath components are purged (106) with liquid carbon dioxide.

Schaltventil, insbesondere Hochdruck-Schaltventil für die Hochleistungsflüssigkeitschromatographie

Schaltventil (a) mit einem in einem Gehäuse (260) angeordneten Stator (202), welcher mehrere Anschlussports aufweist, und (b) mit einem im Gehäuse (260) angeordneten, drehbaren Rotor (210), der in vorbestimmten, durch zugeordnete Winkelstellungen definierten Schaltstellungen mit dem Stator (202) zur fluidischen Verbindung oder Trennung von vorbestimmten Anschlussports zusammenwirkt, (c) wobei der Rotor (210) mittels einer im Gehäuse (260) angeordneten Lager- und Anpresseinrichtung (230) drehbar gelagert und mit einer vorgegebenen Anpresskraft in Richtung auf den Stator (202) beaufschlagt ist, dadurch gekennzeichnet, (d) dass die Lager- und Anpresseinrichtung (230) eine Federeinheit (255) aufweist, (i) welche den Rotor (210) oder ein damit unmittelbar oder mittelbar verbundenes Koppelelement (220) zur Übertragung der Anpresskraft und radialer Kräfte beaufschlagt, und (ii) welche den Rotor (210), oder den Rotor (210) und das damit unmittelbar oder mittelbar verbundene Koppelelement (220), ...

SWITCHING VALVE FOR LIQUID CHROMATOGRAPHY

A switching valve includes a stator and a rotor. The stator includes multiple connection ports. The rotor has predetermined switching positions and interacts with the stator to form a fluidic connection or a fluidic disconnection of predetermined connection ports. The rotor can be mounted rotatably via a bearing and pressing device loaded with a predefined pressing force in a direction of the stator. The bearing and pressing device includes a compensation element to load the rotor and transmit the pressing force. The compensation element is configured to make an elastic flexural deformation so that the compensation element loads the rotor when the rotor is wobbling with respect to an axis of rotation. 1. A switching valve comprising:(a) a stator arranged in a housing, the stator including multiple connection ports;(b) a rotor configured to be rotated, is arranged in the housing and, in which the rotor has predetermined switching positions and interacts with the stator to form a fluidic connection or a fluidic disconnection of predetermined connection ports;(c) the rotor being mounted rotatably via a bearing and pressing device, where the bearing and pressing device is arranged in the housing and loaded with a predefined pressing force in a direction of the stator;(d) in which the bearing and pressing device includes a compensation element to load the rotor and transmit the pressing force; and(e) the compensation element includes a head region, a base region, and a bending region, the head region loads the rotor with a loading face, the base region is supported against a coupling unit of the bearing and pressing device to generate the pressing force or against a coupling element of the bearing and pressing device to generate the pressing force, the bending region disposed between the head region and the base region, and configured to make an elastic flexural deformation so that the head region loads the rotor when the rotor is wobbling with respect to an axis of ...

SWITCHING VALVE FOR HIGH-PERFORMANCE LIQUID CHROMATOGRAPHY

A switching valve is described that has a stator which is arranged in a housing and which has multiple connection ports. The switching valve also has a rotatable rotor which is arranged in the housing and which, in predetermined switching positions defined by associated angular positions, interacts with the stator for the fluidic connection or separation of predetermined connection ports. The rotor is rotatably mounted, and acted on with a predefined pressing force in the direction of the stator, by means of a bearing and pressing device arranged in the housing. The bearing and pressing device has a spring device which exerts a load on the rotor or on an element that is capable of performing a wobbling movement. 1. A switching valve comprising:(a) a stator arranged in a housing, the stator including multiple connection ports;(b) a rotor arranged in the housing and having a switching position to form a fluidic connection to predetermined connection ports;(c) in which the rotor is rotatably mounted and pressed against the stator with a bearing and pressing device arranged in the housing;(d) in which the bearing and pressing device is coupled to the rotor to transmit a pressing force; and(e) in which the rotor is configured to wobble when pressed against the stator.2. The switching valve of claim 1 , in which the bearing and pressing device comprises a spring unit3. The switching valve of further comprising: a coupling element to connect the rotor and the bearing and pressing device.4. The switching valve of claim 2 , in which the rotor is coupled to a rotary drive.5. The switching valve of claim 4 , in which the spring unit is supported on a rotatably mounted receiving element of the bearing and pressing device claim 4 , the receiving element including a drive region coupled to an electromotive rotary drive.6. The switching valve of claim 5 , in which the spring unit is fixedly connected to the receiving element.7. The switching valve of claim 5 , in which the spring ...

VALVE HAVING A DEFORMABLE PART AND USE OF THE VALVE

The invention relates to a valve, in particular a sample injection valve, for a device () for synthesizing, analyzing, and/or separating, comprising at least three liquid connections (), a housing () as a valve part, and a valve body () as another valve part for selectively connecting the liquid connections () by means of at least one flow channel () bounded at least partially by sealing surfaces () between the housing () and the valve body (), wherein the housing () and/or the valve body () are supported in such a way as to be movable relative to each other. In order to create a one-way valve, at least one valve part ( or ) adjacent to the sealing surface () and made of a plastic material can be plastically deformed according to a relative position (), in particular of the valve body (), in order to be able to withstand the elevated pressure loads in the flow channel () in a liquid-tight manner in the relative position (), in particular of the valve body (). 11345677893456771011121011128989891011129910111299. Valve , particularly sample application valve , for an apparatus () for synthesis , analysis and/or separation purposes , having at least three liquid connectors (′ , ′ , ′ , ′ , ′ , ″) , having a housing () as a valve part , and having a valve body () as another valve part , for optional connection of the liquid connectors (′ , ′ , ′ , ′ , ′ , ″) by way of at least one flow channel ( , , ) delimited at least in part by sealing surfaces (′ , ′ , ′) between housing () and valve body () , wherein housing () and/or valve body () are mounted so as to be movable relative to one another , wherein at least one valve part ( or ) that borders on the sealing surface (′ , ′ , ′) and has a plastic material is configured to be plastically deformable , as a function of a relative position (′) , particularly of the valve body () , in order to be able to withstand increased pressure stresses in the flow channel ( , , ) , in liquid-tight manner , in this relative position (′) ...

METHOD OF CLEANING DETECTION CELL OF ELECTRON CAPTURE DETECTOR, ANALYSIS METHOD, DETECTION CELL OF ELECTRON CAPTURE DETECTOR, ELECTRON CAPTURE DETECTOR, AND ANALYTICAL DEVICE

A method of cleaning a detection cell of an electron capture detector in which the detection cell includes a radiation source that emits radiation, a sample gas introduction port through which a sample gas is introduced, and a collector electrode, includes: introducing a cleaning gas into the detection cell of the electron capture detector. 1. A method of cleaning a detection cell of an electron capture detector , the detection cell comprising a radiation source that emits radiation , a sample gas introduction port through which a sample gas is introduced , and a collector electrode , the method comprising:introducing a cleaning gas into the detection cell of the electron capture detector.2. The method of cleaning a detection cell of an electron capture detector according to claim 1 , wherein:the cleaning gas is a gas having a reducing property.3. The method of cleaning a detection cell of an electron capture detector according to claim 2 , wherein:the cleaning gas is hydrogen.4. The method of cleaning a detection cell of an electron capture detector according to claim 1 , wherein:the cleaning gas is introduced into the detection cell that is heated to a temperature of 500° C. or less.5. The method of cleaning a detection cell of an electron capture detector according to claim 2 , wherein:the cleaning gas is introduced into the detection cell that is heated to a temperature of 500° C. or less.6. The method of cleaning a detection cell of an electron capture detector according to claim 3 , wherein:the cleaning gas is introduced into the detection cell that is heated to a temperature of 500° C. or less.7. The method of cleaning a detection cell of an electron capture detector according to claim 1 , wherein:oxidation of the detection cell is prevented by holding the cleaning gas in the detection cell or continuously introducing the cleaning gas into the detection cell.8. The method of cleaning a detection cell of an electron capture detector according to claim 2 , ...

AUTOSAMPLER AND LIQUID CHROMATOGRAPH

An autosampler is provided with a needle, syringe pump, needle drive mechanism, sample loop, and flow path switching mechanism. The flow path switching mechanism has a plurality of solvent delivery flow paths that each deliver a different solvent, an analysis flow path that is in communication with an analysis column for separating a sample, and a plurality of connection ports to which the ends of the sample loop are individually connected, and switches, by switching the Connection state between the connection ports, to either a loading mode for connecting all the solvent delivery flow paths to the analysis flow path without interposing the sample loop or an injecting mode for interposing the sample loop between all the solvent delivery flow paths and the analysis flow path. 16-. (canceled)7. An autosampler comprising:a needle;a syringe pump which sucks and discharges a sample through the needle;a needle drive mechanism which moves the needle;a sample loop which holds the sample sucked by the syringe pump; anda flow path switching mechanism which includes a plurality of solvent supply ports individually connected to a plurality of solvent delivery flow paths feeding different solvents without any merging, an analysis port connected to an analysis flow path communicating with an analysis column for separating the sample, and a plurality of connection ports including a loop one end side port connected to one end of the sample loop and switches a connection state between the connection ports to select any one of a loading mode for connecting all the solvent delivery flow paths to the analysis flow path without using the sample loop therebetween and an injecting mode for interposing the sample loop between all the solvent delivery flow paths and the analysis flow path,wherein the flow path switching mechanism is a rotary type valve which switches a connection state between the connection ports by rotating a rotor including a flow path communicating the connection ports, ...

METHOD FOR FRACTIONATING DIOXINS

In a standing pipe body (), an adsorbent layer () filled with active magnesium silicate as an adsorbent and an alumina layer () positioned therebelow are arranged. A sample solution containing dioxins is applied into the pipe body () from the top, and an aliphatic hydrocarbon solvent is subsequently supplied into the pipe body () from the top. The aliphatic hydrocarbon solvent having dissolved dioxins in the sample solution passes through the adsorbent layer () and the alumina layer () in this order, and is discharged from a bottom of the pipe body (). At this point, a dioxin group including non-ortho PCBs, PCDDs, and PCDFs is selectively trapped by the adsorbent layer (), and mono-ortho PCBs are selectively trapped by the alumina layer (). 1. A method for fractionating dioxins , comprising:a step of passing an aliphatic hydrocarbon solvent solution containing the dioxins to pass through an adsorbent layer using an adsorbent mixed active magnesium silicate.2. The method for fractionating the dioxins according to claim 1 , whereinthe adsorbent further contains graphite mixed with the active magnesium silicate.3. The method for fractionating the dioxins according to claim 2 , whereina content of the graphite in the adsorbent is equal to or lower than 25% by weight.4. The method for fractionating the dioxins according to claim 2 , whereinthe active magnesium silicate is prepared in such a manner that a mixture of magnesium silicate and graphite is heated to equal to or lower than 650° C.5. The method for fractionating the dioxins according to claim 1 , whereinthe aliphatic hydrocarbon solvent solution having passed through the adsorbent layer further passes through an alumina layer.6. The method for fractionating the dioxins according to claim 5 , further comprising:a step of supplying a solvent capable of dissolving the dioxins to the adsorbent layer through which the aliphatic hydrocarbon solvent solution has passed, and then collect the solvent having passed through ...

CHROMATOGRAPHIC PURIFICATION METHOD

A chromatographic purification method for the isolation of a desired product fraction from a mixture using 2 chromatographic columns, comprises, within one cycle to be carried out at least once, the following steps: a first batch step, wherein said columns are disconnected and a first column is loaded with feed and its outlet is directed to waste, and from a second column desired product is recovered and subsequently the second column is regenerated; a first interconnected step, wherein the outlet of the first column is connected to the inlet of the second column, the first column is loaded beyond its dynamic breakthrough capacity with feed, and the outlet of the second column is directed to waste, a second batch step analogous to the first batch step but with exchanged columns; and a second interconnected step, analogous to the first interconnected step but with exchanged columns. 115.-. (canceled)16. A chromatographic purification method for an isolation of target molecules from a feed consisting of the target molecules and impurities to be separated from the target molecules , the target molecules being adsorbed on an affinity chromatography material having immobilized ligands that bind specifically to the target molecules while letting the impurities pass by unaffected , the method using only two chromatographic columns consisting of a first column and a second column , the first column and the second column being loaded with the affinity chromatography material , the first column having a first column inlet and a first column outlet , the second column having a second column inlet and a second column outlet , the method comprising the following steps in order: the two chromatographic columns are disconnected,', 'the first column is loaded with the feed via the first column inlet at a first flow rate and the first column outlet is directed to a waste, and', 'from the second column, the target molecules are recovered via the second column outlet based on the ...

MULTI-MEASUREMENT FLOW CELL ASSEMBLY FOR LIQUID CHROMATOGRAPHY

A detector for detecting constituents of a liquid for use in liquid chromatography is disclosed. The detector includes a first optical flow cell body and a second optical flow cell body, each having a channel therethrough that allows passage of a liquid from an inlet port to an outlet port. The first and second optical flow cell bodies are arranged in series such that the liquid exiting the outlet port of the first optical flow cell body enters the inlet port of the second optical flow cell body. An insulator resides between the first optical flow cell body and the second optical flow cell body, which is adapted to electrically insulate the first optical flow cell body from the second optical flow cell body while allowing the liquid to pass from the first optical flow cell body to the second optical flow cell body. The first optical flow cell body is adapted to facilitate measurement of absorption by the liquid of a first wavelength of light, and second optical flow cell body is adapted to facilitate measurement of absorption by the liquid of a second wavelength of light. The first and second optical flow cell bodies are further adapted to perform as electrodes for measuring the conductivity of the liquid. 1. An LED light source for use with a photodetector , comprising:two or more LED lights in close enough proximity such that they produce essentially coaxial beams of light that diverge by no more than 5 degrees; anda light pulsing controller configured to pulse the beams of LED light, wherein the light pulses occur with cycle times between 0.1 and 500 milliseconds, and with the light pulses occurring over less than about 50% of the cycle.2. The LED light source of claim 1 , wherein the light pulsing controller comprises software for controlling the timing and duration of the pulses of light claim 1 , and the timing and duration of the cycles.3. The LED light source of claim 1 , wherein the light pulsing controller is configured to pulse the beams of LED light ...

System for automatic sampling and detection of on-line gas by high-temperature and high-pressure simulator and detection method thereof

Disclosed is a system for automatic sampling and detection of on-line gas by high-temperature and high-pressure simulator and a detection method thereof. A vacuum manifold I is successively connected to a pressure controller, a gas-liquid separator, a gas automatic metering collector, a first trace quantitative gas collector, and a mechanical pump. On a vacuum manifold II, a high-low pressure convertor, a second trace quantitative gas collector, a gas transferring device, a heavy oil trap, a filtering trap, and a pressure gage head are connected successively. In the present invention, discharged simulation products, enter an empty gas chromatograph at a normal temperature and a normal pressure after treatment, completing on-line detection of an accumulated gas and a stage gas, and improving accuracy of gas composition measurement.

CARRIER FOR AFFINITY CHROMATOGRAPHY

Provided is an affinity chromatography carrier that suppresses nonspecific adsorption of impurities, suppresses a decrease in dynamic binding capacity due to long-term storage, and has high storage stability. 1. An affinity chromatography carrier , the carrier comprising: (M-1) more than 20 parts by mass to 99.5 parts by mass or less of a structural unit derived from an epoxy group-containing monovinyl monomer with respect to all structural units and', '(M-2) 0.5 to 80 parts by mass of a structural unit derived from a polyvinyl monomer with respect to all the structural units;, 'a solid support comprising a copolymer comprisingring-opened epoxy groups obtained by subjecting the epoxy groups to the ring-opening; anda ligand coupled to the solid support,wherein pH in a column vessel packed with the carrier and then purged with a 2 M NaCl-containing 20 mM sodium phosphate buffer with a pH of 7.5 increases by at most 2 after the column stands at 40° C. for 7 days.2. The carrier according to claim 1 , further comprising: (M-3) 40 parts by mass or less of a structural unit derived from an epoxy-free monovinyl monomer with respect to all the structural units.5. The carrier according to claim 3 , wherein X is a thio group (>S) claim 3 , a sulfinyl group (>S═O) claim 3 , or an oxy group (>O).6. The carrier according to claim 1 , wherein the ligand is an immunoglobulin-binding protein.7. The carrier according to claim 6 , wherein the immunoglobulin-binding protein is one or more selected from the group consisting of protein A claim 6 , protein G claim 6 , protein L claim 6 , Fc-binding protein claim 6 , and a functional variant thereof.8. The carrier according to claim 1 , wherein the solid support is a porous particle.9. The carrier according to claim 8 , wherein the porous particle has a particle size of 35 to 100 μm.10. An column claim 8 , comprising:a column vessel; and{'claim-ref': {'@idref': 'CLM-00001', 'claim 1'}, 'the carrier according to ,'}wherein the column vessel ...

FITTINGLESS PNEUMATIC MULTIPORT SELECTOR VALVE FOR ANALYTIC INSTRUMENTS

A pneumatic multiport selector valve and analytic instrument employing said valve are provided. The valve includes a base having a mounting surface configured to mount to the analytic instrument. The mounting surface has a plurality of apertures therein for coupling to flow conduits of the analytic instrument. At least one piston plate has a number of apertures therethrough. The apertures are configured to slidably receive respective pistons. Pistons are disposed in respective apertures of the at least one piston plate. A cushion layer is disposed between the at least one piston plate and the base. The cushion layer has a plurality of apertures to allow actuation gas therethrough. A plate clamps the at least one piston plate to the base. 1. A pneumatic multiport selector valve comprising:a base having a mounting surface configured to mount to an analytic instrument, the mounting surface having a plurality of apertures therein for coupling to flow conduits of the analytic instrument;at least one piston plate having a number of apertures therethrough, the apertures being configured to slidably receive respective pistons;a plurality of pistons disposed in respective apertures of the at least one piston plate;a cushion layer disposed between the at least one piston plate and the base, the cushion layer having a plurality of apertures to allow actuation gas therethrough; anda plate clamping the at least one piston plate to the base.2. The pneumatic multiport selector valve of claim 1 , and wherein the cushion layer is isolated from the actuation gas.3. The pneumatic multiport selector valve of claim 2 , wherein the cushion layer is porous.4. The pneumatic multiport selector valve of claim 3 , wherein the cushion layer is formed of a meta-aramid material.5. The pneumatic multiport selector valve of claim 3 , and further comprising a plurality of seals isolating the cushion layer from the actuation gas.6. The pneumatic multiport selector valve of claim 1 , wherein the ...

Connector with structural reinforcement and biocapatible fluid passageway

A connector with a biocompatible fluid passageway and method for manufacturing thereof is described. The biocompatible connector may be used in an analytical instrument (AI) system as a union, an adapter, a tee, a cross, a manifold, a valve, or for other fittings or components. The connector has a reinforcement insert and a biocompatible molding covering portions of the reinforcement insert. The reinforcement insert has a first portion, a second portion, and a middle portion between the first portion and the second portion. The first and second portions have threaded sections and each have a plurality of non-threaded sections. For a given portion, the junction of the non-threaded sections forms a lip by which to prevent the molded material from flowing into the threaded sections. In certain embodiments, an interior web is used in the reinforcement insert to provide additional structural support. 1. A connector for use with an analytical instrument system , comprising: [ i) the first portion comprising a first end, a second end, an interior threaded section proximal the first end of the first portion, a first interior non-threaded section connected to the interior threaded section, a second interior non-threaded section connected to the first interior non-threaded section, and an exterior surface,', 'ii) wherein the junction of the first interior non-threaded section and the second interior non-threaded section forms a first portion lip,, 'a) a first portion proximal to the reinforcement insert first end,'}, i) the second portion comprising a first end, a second end, an interior threaded section proximal the first end of the second portion, a first interior non-threaded section connected to the first interior threaded section, a second interior non-threaded section connected to the first interior non-threaded section, and an exterior surface,', 'ii) wherein the junction of the first interior non-threaded section and the second interior non-threaded section forms a ...

LIQUID CHROMATOGRAPH AND ANALYSIS EXECUTION METHOD

When a flow path switch valve is in a first flow path state, an aqueous solvent supplied by an aqueous solvent supplier is guided to a first flow path, and a pH of the aqueous solvent is measured by a pH meter provided in the first flow path. When the flow path switch valve is in a second flow path state, the aqueous solvent supplied by the aqueous solvent supplier is guided to a second flow path. A sample to be analyzed is supplied by a sample supplier at a position farther downstream than the flow path switch valve. The solvent that has passed through the second flow path and the sample supplied by the sample supplier are guided to a separation column. The sample that has passed through the separation column is detected by a detector. 1. A liquid chromatograph comprising:a first flow path;a second flow path;a first aqueous solvent supplier that supplies an aqueous solvent;a flow path switch valve that is switchable between a first flow path state where the aqueous solvent supplied by the first aqueous solvent supplier is guided to the first flow path and a second flow path state where the aqueous solvent supplied by the first aqueous solvent supplier is guided to the second flow path;a pH meter that is provided in the first flow path and measures a pH of the aqueous solvent flowing through the first flow path when the flow path switch valve is in the first flow path state;a sample supplier that supplies a sample to be analyzed at a position farther downstream than the flow path switch valve;a separation column into which the solvent that has passed through the second flow path and the sample supplied by the sample supplier are introduced; anda detector that detects the sample that has passed through the separation column.2. The liquid chromatograph according to claim 1 , further comprising:an organic solvent supplier that supplies an organic solvent; anda mixer that mixes the aqueous solvent flowing through the second flow path when the flow path switch valve is ...

Modifier stream elution of trap column for multidimensional compressible fluid-based chromatography

A method of performing a chromatographic separation includes modulating a portion of a flowstream to a trap column to retain at least one analyte from the flowstream on the trap column. A flow of a modifier is provided through the trap column to generate an elution comprising the at least one analyte. A flow of a compressible fluid-based chromatography (CFC) mobile phase or CFC solvent is merged with the elution from the trap column to generate a diluted elution. Carbon dioxide may be used as the CFC solvent or as a component of the CFC mobile phase. The diluted elution is provided to a CFC column where at least one analyte is focused at a head of the CFC column. Examples of a flowstream that may be used include an eluent from a chromatography column or a fluid flow from an extraction system.

Decomposition Method and Apparatus Based on Basis Material Combination

The present disclosure relates to a gas purification apparatus and a trace substance detection device. The gas purification apparatus includes a first purification component, a second purification component and a switching component, wherein the switching component can be switched between a first state and a second state, the first purification component and a component to be purified form a gas purification loop in the first state, and the second purification component can provide a regeneration gas for the first purification component in the second state, so that water vapor and impurities in the first purification component are discharged to outside. In the gas purification apparatus, the filtered air is used as the regeneration gas to prevent secondary pollution in a recycling process of the purificant; furthermore, by means of the state switching function of the switching component, the mutual interference between the two working states of purification and regeneration can be prevented, and all the above advantages can improve the reliability of the recycling of the purificant, thereby optimizing the performance and the service life of the gas purification apparatus. 1. A gas purification apparatus , comprising:a first purification component;a second purification component; anda switching component configured to switch between a first state and a second state;in the first state, the first purification component and a component to be purified form a gas purification loop; andin the second state, the second purification component provides a regeneration gas for the first purification component, so that water vapor and impurities in the first purification component are discharged to outside.2. The gas purification apparatus according to claim 1 , wherein the switching component comprises a switching valve.3. The gas purification apparatus according to claim 2 , wherein the switching component comprises a first switching valve and a second switching valve; when ...

METHOD OF TRANSMITTING CONTROL DATA FOR SYSTEM CONVERSION AMONG LIQUID CHROMATOGRAPHS

In the present invention, differences in liquid feed properties between liquid chromatographic devices are determined, a liquid feed time table that takes these differences into account is acquired by way of system changes, and flow control is performed by a pump. Furthermore, the liquid feed time table after the system changes is divided into a plurality of intervals, and instructions are sent to the pump after approximate calculations are performed. Thus, by efficiently performing system change processes, the same measurement results can be obtained in a plurality of different liquid chromatograph devices, regardless of the differences in liquid feed properties. 1. A liquid chromatograph comprising:a liquid chromatograph unit which includes an elution unit sending an eluent to a detecting unit; anda control unit which controls elution performed by the elution unit based on a predetermined time table, a storage unit which stores an elution response of the liquid chromatograph unit to be obtained when a predetermined command value is input to the elution unit and an elution response of another liquid chromatograph to be obtained when the command value is input to another elution unit of another liquid chromatograph,', 'a first processing unit which converts the time table based on an elution profile and another elution response such that the elution response at the time when the elution unit is controlled by the liquid chromatograph unit based on the time table approaches another elution profile at the time when another elution unit is controlled by another liquid chromatograph based on the time table, and', 'a second processing unit which divides the converted time table into a plurality of regions, and, 'wherein the control unit includes'}the second processing unit performs approximate calculation for each of the divided regions and supplies a signal to the elution unit based on the result of the approximate calculation.2. The liquid chromatograph according to ...

SYSTEMS, METHODS, AND DEVICES PROVIDING SOLVENT CONTAINER IDENTIFICATION AND INCORPORATION

Solvent containers and solvent container trays for chromatography systems are described for providing control over solvent supply and waste collection. Designated solvent containers and exclusively designated solvent containers provide solvents for use by chromatography systems. Control over solvent supply is achieved by requiring matched container shape and container receiving position shape within the tray, and additionally or alternatively, through solvent container coding readable by the solvent tray and chromatography system which provide information about the solvent container to the chromatography system. 1. A solvent container system for a chromatography system , wherein the solvent container system comprises:a plurality of container types, each container type comprising a container body and describing a unique shape, anda tray comprising a plurality of receiving positions, each receiving position formed to correspond to one of the unique shapes, such that the tray may have one or more receiving positions corresponding to each of the unique shapes represented by the plurality of container types, or corresponding to each of a subset of the unique shapes represented by the plurality of container types.2. A solvent container system for a chromatography system , wherein the solvent container system comprises:a set of coded containers, each coded container comprising a container body and an identification code unit providing an identification code, andone or more receiving positions, each receiving position comprising a reader which reads the identification code.3. The solvent container system of claim 2 , wherein the identification code unit is selected from a group consisting of a bar code claim 2 , a matrix bar code claim 2 , a radio-frequency identification (RFID) device claim 2 , a 125 kHz or 13.56 MHz proximity device claim 2 , and a contact smart chip.4. The solvent container system of claim 2 , wherein the identification code unit is a set of tab ...

LIQUID CHROMATOGRAPH AND LIQUID CHROMATOGRAPH ANALYSIS METHOD

A liquid chromatograph analysis method and a liquid chromatograph minimize analysis time when performing analyses by switching columns and mobile phases. Liquid chromatograph for performing a plurality of analyses according to a schedule table includes: mobile phase switching sections for switching a plurality of mobile phases to select a mobile phase to be used in analysis; column switching sections for switching a plurality of columns -to select a column to be used in analysis; and control section including memory for storing an equilibration time of each of the plurality of columns to and an equilibration controller for controlling column equilibration. If used columns or used mobile phases are different between two consecutively executed analyses, equilibration controller equilibrates a column used in the later of the two analyses over an equilibration time read out from memory 1. A liquid chromatograph analysis method in which a sample is analyzed using a liquid chromatograph provided with a function of switching a plurality of columns and a plurality of mobile phases , according to a schedule table in which analysis conditions and execution order of a plurality of analyses are described , the analysis method comprising the steps of:a) storing an equilibration time of each of the plurality of columns in a memory;b) reading out, from the memory, an equilibration time of a column used in later one of two analyses consecutively executed in the schedule table, in a case where used columns or used mobile phases are different between the two analyses; andc) equilibrating the column used in the later analysis over the equilibration time read out from the memory, immediately before the later analysis is performed.2. The liquid chromatograph analysis method according to claim 1 , further comprising setting the equilibration time of the column used in the later analysis to a default value claim 1 , in a case where the read-out of the equilibration time of the column used ...

MULTI-MEASUREMENT FLOW CELL ASSEMBLY FOR LIQUID CHROMATOGRAPHY

A detector for detecting constituents of a liquid for use in liquid chromatography is disclosed. The detector includes a first optical flow cell body and a second optical flow cell body, each having a channel therethrough that allows passage of a liquid from an inlet port to an outlet port. The first and second optical flow cell bodies are arranged in series such that the liquid exiting the outlet port of the first optical flow cell body enters the inlet port of the second optical flow cell body. An insulator resides between the first optical flow cell body and the second optical flow cell body, which is adapted to electrically insulate the first optical flow cell body from the second optical flow cell body while allowing the liquid to pass from the first optical flow cell body to the second optical flow cell body. The first optical flow cell body is adapted to facilitate measurement of absorption by the liquid of a first wavelength of light, and second optical flow cell body is adapted to facilitate measurement of absorption by the liquid of a second wavelength of light. The first and second optical flow cell bodies are further adapted to perform as electrodes for measuring the conductivity of the liquid. 1. A flow cell system comprising:a) a first optical flow cell body comprising an inlet port, an outlet port, and a channel through the optical flow cell body connecting the inlet port to the outlet port,b) a second optical flow cell body comprising an inlet port, an outlet port, and a channel through the optical flow cell body connecting the inlet port to the outlet port,c) a housing which supports the first optical flow cell body and the second optical flow cell body, such that the first and second optical flow cell bodies are in series, with the outlet port of the first optical flow cell body adjacent to the inlet port of the second optical flow cell body,d) a first conductive material adjacent to the outlet port of the first optical flow cell body, positioned ...

FLOW CELL

A flow cell including a flow cell bod having a flow path formed therein; an optically transmissive member disposed at a part wherein light from the light source is incident into, or emitted from, the flow path; a shock absorbing member that is disposed between the optically transmissive member and the cell body; an elastic member, made from a metal material, for pressing the optically transmissive member toward the cell body side; and a fastening tool, attached to the cell body, for positioning the elastic member. 1. A flow cell comprising:a cell body having a flow path formed therein;an optically transmissive member disposed at a part within the flow path wherein light from a light source is incident or emitted;a sealing member disposed between the optically transmissive member and the cell body;an elastic member, made from a metal material, for pressing the optically transmissive member in the direction of the cell body; anda fastening tool that is attached to the cell body for positioning the elastic member.2. A flow cell as set forth in claim 1 , wherein:the optically transmissive member is a lens or a window plate.3. A flow cell as set forth in claim 1 , wherein:the fastening tool is a fastening screw having a hole at the light path of the light.4. A flow cell as set forth in claim 1 , wherein:the elastic member has a disk spring shape.5. A flow cell as set forth in claim 4 , wherein:the fastening tool is a fastening screw, and an elastic member having a disk spring shape is integrated with the fastening screw.6. A flow cell as set forth in claim 1 , wherein:the elastic member is a compression spring.7. A flow cell as set forth in claim 1 , wherein:a shock absorbing member made from resin is disposed between the optically transmissive member and the elastic member; andthe elastic member presses the optically transmissive member toward the cell body side through the shock absorbing member. The present invention relates to a flow cell.Light absorption detecting ...

Fluid supply devices and fluid member for forming a mobile phase for a sample separating device

A fluid supply device for providing a mobile phase for a sample separating device includes, a supply conduit for providing a fluid which forms at least a part of the mobile phase, a fluid valve which is fluidically coupled with the supply conduit and, depending on its switching state, enables or prevents a passing of the fluid from the supply conduit, an elastic buffer unit which is fluidically coupled upstream of the fluid valve with the supply conduit and which is configured for buffering the fluid, and a fluid conveying unit for conveying the fluid which passes the fluid valve.

COATED FLOW PATH COMPONENTS FOR CHROMATOGRAPHIC EFFECTS

The present disclosure relates to the determination of analytes in a sample using chromatography. The present disclosure provides methods of separating an analyte from a sample. The method includes introducing a sample comprising the analytes into a chromatographic system. The chromatographic system has a flow path disposed in an interior of the chromatographic system, at least a portion of the flow path having an active coating, and a chromatographic column having a stationary phase material in an interior of the chromatographic column that facilitates separation of the analytes in the sample through interaction with at least one analyte in the sample. The active coating is selected to interact with at least one analyte in the sample through (1) a repulsive force, (2) a secondary interaction, or (3) a retention mechanism distinct from the interaction with the stationary phase material. 1. A method of separating analytes in a sample , the method comprising: a flow path disposed in an interior of the chromatographic system, at least a portion of the flow path having an active coating; and', 'a chromatographic column having a stationary phase material in an interior of the chromatographic column that facilitates separation of the analytes in the sample through interaction with at least one analyte in the sample;, 'introducing a sample comprising the analytes into a chromatographic system comprisingwherein the active coating is selected to interact with at least one analyte in the sample through (1) a repulsive force, (2) a secondary interaction, or (3) a retention mechanism distinct from the interaction with the stationary phase material.2. The method of claim 1 , wherein the analyte and the active coating are negatively charged.3. The method of claim 1 , wherein the analyte and active coating are positively charged.4. The method of claim 1 , wherein the active coating is applied to the flow path by vapor deposition.5. The method of claim 1 , wherein active coating is ...

Method and System for Determining the Influence of Experimental Parameters on a Liquid Chromatography Protocol

Method for determining the influence of least a first and a second experimental parameter on a liquid chromatography protocol for purifying one or more target molecules from a sample, comprising the steps: —performing chromatography purifications of the sample at a plurality of different experimental conditions where at least the first and the second experimental parameter each are varied over a predetermined range, each purification being registered as a chromatogram by monitoring an output parameter indicative of the purification result during the purification; and —displaying in a graphical user interface at least a subset of the registered chromatograms as chromatogram-miniatures in an evaluation diagram wherein the position of each displayed chromatogram-miniature is determined by the experimental parameters for the corresponding purification, thereby allowing a user to visually determine trends and the influence of the experimental parameters on the liquid chromatography protocol. 1. Method for determining the influence of least a first and a second experimental parameter on a liquid chromatography protocol for purifying one or more target molecules from a sample , comprising the steps:performing chromatography purifications of the sample at a plurality of different experimental conditions where at least the first and the second experimental parameter each are varied over a predetermined range, each purification being registered as a chromatogram by monitoring an output parameter indicative of the purification result during the purification; anddisplaying in a graphical user interface at least a subset of the registered chromatograms as chromatogram-miniatures in an evaluation diagram wherein the position of each displayed chromatogram-miniature is determined by the experimental parameters for the corresponding purification, thereby allowing a user to visually determine trends and the influence of the experimental parameters on the liquid chromatography ...

THIN-LAYER CHROMATOGRAPHY SYSTEM AND METHOD FOR ASSESSING ANALYTE CONCENTRATIONS IN SAMPLES

The invention relates to cartridges for thin layer chromatography (TLC) which hermetically seal the TLC mobile phase during loading, performance of the TLC process, and analysis of the chromatogram. It further relates to associated methods of use—for example for detecting or quantifying an analyte in a sample. It further relates to processes for assembling the cartridge, to associated equipment adapted for use with them, and kits of parts. 1. A method for detecting or quantifying an analyte in a sample , the method comprising:(i) providing said sample;(ii) preparing an analysis sample from said sample;(iii) providing a thin layer chromatography (TLC) cartridge comprising the TLC plate and a sealed reservoir containing the TLC mobile phase;(iv) loading the analysis sample and one or more reference standards onto the TLC plate of the cartridge;(v) exposing the TLC plate to the TLC mobile phase to perform TLC on said analysis sample in the TLC cartridge to produce a chromatogram;(vi) optically analysing analyte and reference standards bands on the chromatogram, with an imaging device to generate optical signals corresponding to the analyte and reference standards; 'characterised in that the TLC cartridge hermetically seals the TLC mobile phase during loading, performance of the TLC process, and analysis of the chromatogram.', '(vii) detecting or quantifying the analyte in the sample according to said optical signals,'}2. A method as claimed in wherein step (v) comprises in any appropriate order:(v-1) breaking a barrier between the mobile phase reservoir and the TLC plate to allow fluid access between them;(v-2) orientating the cartridge to a first orientation, which first orientation permits the base of the plate to be exposed to the released mobile phase solvent and start the TLC process;(v-3) during or after production of the chromatogram, orientating the cartridge to a second orientation, which second orientation ceases exposure of the base of the plate to the ...

LIQUID CHROMATOGRAPH

A liquid chromatograph includes: a concentration column for capturing a target component; an analysis column for separating the target component from other components; first passage-switching units for switching between the state of forming a passage through which a sample-introduction mobile phase containing the liquid sample is fed to the concentration column, and the state of forming a passage through which an eluant for eluting the target component captured in the concentration column is fed to the concentration column; a storage unit for storing the eluant; and a second passage-switching unit for switching between the state of forming a passage through which an analysis mobile phase is fed to the analysis column and a passage through which the eluant from the concentration column is fed to the storage unit, and the state of forming a passage through which analysis mobile phase is fed to the analysis column via the storage unit. 1. A liquid chromatograph comprising:a) a concentration column for capturing a target component in a liquid sample;b) an analysis column for separating the target component from other components;c) a first passage-switching unit configured to switch between a state of forming a passage through which a sample-introduction mobile phase containing the liquid sample is fed to the concentration column, and a state of forming a passage through which an eluant for eluting the target component captured in the concentration column is fed to the concentration column;d) a first storage unit configured to store the eluant containing the target component; ande) a second passage-switching unit configured to switch between a state of forming a passage through which an analysis mobile phase is fed to the analysis column and a passage through which the eluant from the concentration column is fed to the first storage unit, and a state of forming a passage through which the analysis mobile phase is fed to the analysis column via the first storage unit.2. ...

APATITE IN-SITU RESTORATION

The present invention discloses methods of regenerating apatite surfaces, for example after purification of a target analyte. 1. A method of purifying a target analyte with an apatite solid surface , the method comprising:(a) contacting the apatite solid surface with the target analyte, thereby separating the target analyte from one or more contaminants;(b) collecting the target analyte; and(c) after the collecting and before subsequent loading of additional target analyte, regenerating the apatite solid surface, the regenerating comprising:(i) contacting the apatite solid surface with a buffered calcium solution comprising a calcium ion at a concentration of at least 5 mM and a zwitterionic buffer, wherein the ratio of zwitterionic buffer concentration to calcium ion concentration is at least 2, and the pH of the buffered calcium solution is at least 5;(ii) after (i), contacting the apatite solid surface with a phosphate buffered solution at a pH of at least 6.5; and(iii) after (ii), contacting the apatite solid surface with a solution comprising an hydroxide.2. The method of claim 1 , wherein (a) comprises binding the target analyte to the apatite solid surface claim 1 , and (b) comprises eluting the target analyte from the apatite solid surface.3. The method of claim 1 , wherein (a) comprises contacting the apatite solid surface with the target analyte claim 1 , thereby flowing the target analyte through the apatite solid surface claim 1 , and (b) comprises collecting the target analyte in the flow through.4. The method of claim 1 , wherein the zwitterionic buffer is a sulfonic acid containing buffer.5. The method of claim 4 , wherein the sulfonic acid containing buffer is MES claim 4 , PIPES claim 4 , ACES claim 4 , MOPSO claim 4 , MOPS claim 4 , BES claim 4 , TES claim 4 , HEPES claim 4 , DIPSO claim 4 , TAPS claim 4 , TAPSO claim 4 , POPSO claim 4 , or HEPPSO claim 4 , EPPS claim 4 , CAPS claim 4 , CAPSO claim 4 , or CHES.6. The method of claim 5 , wherein the ...

High Throughput Gas-Chromatography System for Additive Analysis, and Analysis Method Using Same

A high speed treatmenthigh throughput gas-chromatography system for analysis of high-molecular weight additives in a polymer material, and an analysis method using same are provided. A high throughput gas-chromatography system is capable of qualitatively and quantitatively analyzing, at the same time, high-molecular weight additives in a polymer, and reducing analysis time by increasing a heating rate and a column maximum temperature.

METHOD FOR PURIFYING ANTIBODY

An antibody purification method, including bringing a solution containing an antibody and HCP into contact with a chromatography media to separate the antibody and the HCP, wherein the chromatography media contains a base media containing porous particles, and a compound having a plurality of primary amino groups bonded to the base media, and 20 to 55% of the primary amino groups in the compound having the plurality of primary amino groups are modified with a hydrophobic group, and collection efficiency of the antibody is 85% or more, and an amount of the HCP in an antibody solution after purification is less than 45 ppm. 1. An antibody purification method , comprising bringing a solution containing an antibody and host cell protein into contact with a chromatography media to separate the antibody and the host cell protein ,wherein the chromatography media contains a base media containing porous particles, and a compound having a plurality of primary amino groups bonded to the base media, and 20 to 55% of the primary amino groups in the compound having the plurality of primary amino groups are modified with a hydrophobic group, andcollection efficiency of the antibody is 85% or more, and an amount of the host cell protein in an antibody solution after purification is less than 45 ppm.2. The method according to claim 1 , wherein the method is performed in a flow-through mode.3. The method according to claim 1 , whereinmore than 40% of primary amino groups in the compound having the plurality of primary amino groups are modified with the hydrophobic group.4. The method according to claim 1 , wherein the compound having the plurality of primary amino groups is selected from the group of polyallylamine claim 1 , polyvinylamine claim 1 , chitosan claim 1 , polylysine claim 1 , polyguanidine and polyornithine.5. The method according to claim 4 , wherein the compound having the plurality of primary amino groups is polyallylamine.6. The method according to claim 5 , wherein ...

PREPARATIVE LIQUID CHROMATOGRAPH

A preparative liquid chromatograph includes a liquid chromatograph section, a trap section, an eluent supply section, a collector, and a flow path switching section. The flow path switching section is configured to be selectively switched to a component trap mode that connects the liquid chromatograph section and the trap section in such a way that a sample component separated in a separation column is trapped by a trap column of the trap section; and a collection mode that connects the eluent supply section and the trap section and connects the trap section and the collector in such a way that the components trapped in the trap column are eluted by an eluent from the eluent supply section and are guided to the collector. 17-. (canceled)8. A preparative liquid chromatograph comprising:a liquid chromatograph section comprising a sample injection part, a separation column, and a detector, wherein the liquid chromatograph section is configured in such a way that a sample injected by the sample injection part is separated into components in the separation column and the separated components are detected by the detector;a trap section that includes one or more trap columns and is configured in such a way that the components separated in the separation column are trapped by the trap columns;an eluent supply section for supplying an eluent for eluting the components trapped in the trap columns from the trap columns;a collector for collecting the eluate from the trap columns;a flow path switching section configured to be selectively switched to a component trap mode and a collection mode, wherein the component trap mode is a mode where the liquid chromatograph section and the trap section are connected to each other in such a way that the components separated in the separation column are trapped by the trap columns, and wherein the collection mode is a mode where the eluent supply section and the trap section are connected to each other, and the trap section and the ...

Directed-flow assay device

A diagnostic assay device that directs an applied sample to the analytical membrane of a directed flow device. The device has a sample receiving port defined by layers of built-up material on one end of the test strip. The port contains the sample and specifically directs it to the membrane in a controlled fashion. Additional features include configuration of the housing in a general C-shape with the test strip spanning the opening of the C-shape to allow access by a reader device. A preferred method employs superparamagnetic particles to label the target analytes for detection and measurement by means of an electromagnetic reader device.

Directed-flow assay device

A diagnostic assay device that directs an applied sample to the analytical membrane of a directed flow device. The device has a sample receiving port defined by layers of built-up material on one end of the test strip. The port contains the sample and specifically directs it to the membrane in a controlled fashion. Additional features include configuration of the housing in a general C-shape with the test strip spanning the opening of the C-shape to allow access by a reader device. A preferred method employs superparamagnetic particles to label the target analytes for detection and measurement by means of an electromagnetic reader device.

Control of sample separation based on analysis of mobile phase supply from mobile phase container

A method of controlling a sample separation apparatus, for separating a fluidic sample using a mobile phase provided from at least one mobile phase container, includes determining a weight and volume reduction behavior according to which weight and volume of mobile phase in a mobile phase container are reduced during conveying mobile phase from the mobile phase container in the sample separation apparatus, and determining a tare weight of the mobile phase container based on a gross weight information, a volume information, and the determined weight and volume reduction behavior. The gross weight information is indicative of an initial gross weight of the mobile phase container including its mobile phase, and the volume information is indicative of an initial mobile phase volume in the mobile phase container.

二维液相色谱系统

二维液相色谱系统具备第一维分析部、第二维分析部、第二维送液装置、分割导入部、位移梯度程序制作部、送液控制部以及位移定时调整部。位移梯度程序制作部构成为制作用于使所述第二维送液装置执行位移梯度送液的位移梯度程序。位移定时调整部构成为：基于在所述一系列分析动作前由所述第一维检测器针对该分析对象试样获取到的预备色谱，来调整由所述位移梯度程序制作部制作出的位移梯度程序中的向各梯级位移的位移定时。

薄层色谱板及使用了其的试料的分析方法

提供能够更简便地、并且在短时间内将多个成分彼此分离的薄层色谱板。薄层色谱板(100)具备基板(10)、和配置于基板(10)上且用于将试料中所含的多个成分彼此分离的分离层(20)。分离层(20)具有沿第一展开方向(X)延伸的带状的第一层(31)、和沿与第一展开方向(X)正交的第二展开方向(Y)延伸的第二层(32)。第二层(32)与第一层(31)相接。第一层(31)由亲水性的多孔质体构成。第二层(32)由疏水性的多孔质体构成。

用于阳离子色谱法的膜和柱的制造方法

本发明的一个目的是在温和条件下制造一种弱酸性阳离子交换剂。本发明的另一个目的是制造一种更牢固的弱酸性阳离子交换膜。本发明的又一个目的是提供一种弱酸性阳离子交换剂，该交换剂能够实现高水平地分离单价阳离子并且同时分析单价阳离子和二价阳离子；并且还提供一种使用该离子交换剂的色谱柱。在本发明弱酸性阳离子交换剂的制造方法中，使用不能溶解分子中带有双键的聚合物的溶剂，弱酸性阳离子交换剂在100℃或更低的温度下通过聚合制造。当α，β-不饱和二元酸溶于该溶剂时，α，β-不饱和二元酸衍生物与聚合物发生反应，形成更牢固的膜。而且，当将由此方法得到的弱酸性阳离子交换剂装填到柱中时，能够以高水平分离单价阳离子。

Method for determining cefotaxime by reversed-phase high-performance liquid chromatography

FIELD: technological processes.SUBSTANCE: present invention relates to a method of determining cefotaxime by reversed-phase high-performance liquid chromatography, comprising an isocratic mode of elution using a chromatographic column filled with a sorbent with particle size of 5 mcm, used as a mobile phase is a mixture of a solution of ammonium acetate with acetonitrile, characterized by the fact that chromatographic separation is carried out on a column with size of 250×3 mm, filled with sorbent C18, using as a mobile phase mixture of 0.02 M ammonium acetate solution pH = 4.7 with acetonitrile in ratio of 90:10 using ultraviolet detector at wave length 252 nm and volume of introduced sample 10 mcl.EFFECT: effective separation of cefotaxime with impurity compounds of the sample and providing high retention of cefotaxime, which increases sensitivity and resolution of the method, reduced consumption of the mobile phase, which increases cost-effectiveness of analysis.1 cl, 2 dwg, 1 ex РОССИЙСКАЯ ФЕДЕРАЦИЯ (19) RU (11) (13) 2 687 493 C1 (51) МПК G01N 30/02 (2006.01) G01N 30/10 (2006.01) G01N 30/26 (2006.01) G01N 30/50 (2006.01) G01N 30/74 (2006.01) C07D 501/06 (2006.01) ФЕДЕРАЛЬНАЯ СЛУЖБА ПО ИНТЕЛЛЕКТУАЛЬНОЙ СОБСТВЕННОСТИ C07D 501/24 (2006.01) C07D 417/12 (2006.01) (12) ОПИСАНИЕ ИЗОБРЕТЕНИЯ К ПАТЕНТУ (52) СПК G01N 30/02 (2019.02); G01N 30/10 (2019.02); G01N 30/26 (2019.02); G01N 30/50 (2019.02); G01N 30/74 (2019.02); C07D 501/06 (2019.02); C07D 501/24 (2019.02); C07D 417/12 (2019.02) (21) (22) Заявка: 2018135531, 08.10.2018 08.10.2018 Дата регистрации: Приоритет(ы): (22) Дата подачи заявки: 08.10.2018 (45) Опубликовано: 14.05.2019 Бюл. № 14 C 1 (73) Патентообладатель(и): Федеральное казённое учреждение здравоохранения "Ставропольский научно-исследовательский противочумный институт" Федеральной службы по надзору в сфере защиты прав потребителей и благополучия человека (RU) (56) Список документов, цитированных в отчете о поиске: N. Lalitha, P.S. Pai: "Development R U 2 6 ...

Preparing method of filler endcapped highly with a good acid resisting and filler and packing column using same

A process for producing a filler improved in durability under the conditions of use of a strongly acidic solvent so that the polar compound is not adsorbed by highly end-capping the silanol group on the surface of the silica gel in preparing a hydrocarbon-based filler for HPLC, And a packed column. The present invention relates to a method for producing a hydrocarbon-based silica gel filler, which comprises the steps of: coupling silica gel with chlorosilane or alkoxysilane containing an alkyl group having 2 to 30 carbon atoms or an aryl group; And a first step of replacing the unreacted chloro group or alkoxy group with a silanol group after the coupling step, and a filler and a packing column prepared using the same, to provide.

Flow path switching valve and liquid chromatograph apparatus using the valve

(Electrolytic eluent modification device for suppressed ion chromatography and ion chromatography system with the same

The present invention relates to an online automatic modification device for a suppressor-type ion chromatography eluent and an ion chromatography system having the same. The device includes: a main body where an ion exchange space portion provided on one side is provided with a supply path for deionized water or eluent supply and a discharge path is formed for outward discharge of the deionized water or eluent after cation or anion addition in the ion exchange space portion; a storage tank provided in the main body so as to be connected to the ion exchange space portion and storing an electrolyte solution for the cation or anion addition; an ion exchange portion allowing either the cation or the anion to pass by separation between the supply path and storage tank sides in the ion exchange space portion; first and second electrodes respectively installed on the supply path side and the storage tank side across the ion exchange portion and respectively supplying anode and cathode currents such that the cation or anion provided from the electrolyte solution is supplied to the supply path side; and a current supply control unit controlling the current supplied to the first and second electrodes. According to the present invention, automatic ionization equilibrium shift of water causing a decline in sensitivity and a nonlinear response in a low concentration region in suppressor-type ion chromatography is suppressed and addressed. Accordingly, the linear range of a calibration curve can be extended to a lower region and detection power can be improved.

Sample injector, sample injecting method and liquid chromatography equipment

본 발명은 스위칭 밸브를 이용해서 이동상의 흐름을 제어하는 시료 주입 장치, 시료 주입 방법 및 액체 크로마토그래피 장치에 관한 것으로, 분리용 칼럼에 접속된 포트와, 이동상을 공급하는 펌프와, 1쌍의 시료 주입용 니들과, 시린지와, 시료 주입용 니들을 펌프 또는 시린지 중 어느 한쪽에 선택적으로 접속함과 아울러, 다른쪽의 시료 주입용 니들을 펌프로 하는 밸브를 갖는다. 그리고, 밸브를 조작함으로써, 시료 주입용 니들이 포트에 장착되었을 때에 이 시료 주입용 니들을 펌프에 접속한다. 또한, 다른쪽의 시료 주입용 니들이 포트에 장착되었을 때 시료 주입용 니들을 시린지에 접속함과 아울러 다른쪽의 시료 주입용 니들을 펌프에 접속하는 구성으로 한다. The present invention relates to a sample injection device, a sample injection method, and a liquid chromatography device for controlling the flow of a mobile phase using a switching valve, comprising a port connected to a separation column, a pump for supplying a mobile phase, and a pair of samples. An injection needle, a syringe, and a sample injection needle are selectively connected to either a pump or a syringe, and have a valve for pumping the other sample injection needle. Then, the sample injection needle is connected to the pump when the sample injection needle is attached to the port by operating the valve. Moreover, when the other sample injection needle is attached to the port, the sample injection needle is connected to the syringe and the other sample injection needle is connected to the pump. 시료 주입 Sample injection

Microfluidic device and microfluidic analysis equipment

본 발명은 미세 유체 소자 및 미세 유체 분석 장치에 관한 것으로, 이 소자는 양측에 형성된 가이드들과, 가이드들 사이에 형성된 유로를 포함하는 하판과, 하판 상에서 가이드들을 따라 이동하되 유로의 길이보다 짧은 길이를 가지는 이동상판을 포함하며, 이동상판의 위치를 조절함으로써 간단하고 정밀하게 유체의 흐름을 제어할 수 있다. 이로써 감지부나 반응부에서 유체의 반응이 충분히 이루어지도록 조절할 수 있어, 유체의 적은 양으로도 효과적인 반응 및 감지를 구현할 수 있으며, 감도를 향상시킬 수 있다. 또한 감도향상으로 반응에 참여하지 않은 물질을 제거하는 세척(washing) 과정을 생략할 수 있다. 또한 상기 이동 상판은 손가락으로 수동으로 조절할 수도 있다. The present invention relates to a microfluidic device and a microfluidic analysis device, comprising: a lower plate including guides formed on both sides, a flow path formed between the guides, and a length shorter than a length of the flow path moving along the guides on the lower plate; It includes a mobile plate having a, it is possible to control the flow of the fluid simply and precisely by adjusting the position of the mobile plate. This can be adjusted so that the reaction of the fluid in the sensing unit or the reaction unit sufficiently, it is possible to implement an effective reaction and detection even with a small amount of fluid, it is possible to improve the sensitivity. In addition, it is possible to omit a washing process to remove a material that does not participate in the reaction due to improved sensitivity. The moving top plate may also be manually adjusted with a finger. 미세유체소자, 분석장치, 랩온어칩, 표면장력 Microfluidic Device, Analysis Device, Lab-on-a-Chip, Surface Tension

Mixing system for a liquid chromatography system

Die Erfindung betrifft ein Mischersystem (1) für ein System zur Flüssigkeitschromatographie, mit einem Mischerblock (10), mehreren Fluideingängen (2), die durch jeweils einen Kanal (3) mit einer Mischkammer (4) verbunden sind, und einem Fluidausgang (5) für gemischtes Fluid, wobei jedem Kanal (3) ein Ventil (6) zugeordnet ist, das den Fluiddurchfluss steuern kann, dadurch gekennzeichnet, dass für jedes Ventil (6) ein Adapter (14) vorgesehen ist, der am Mischerblock (10) angebracht ist und durch den einer der Kanäle (3) verläuft, wobei das Ventil (6) am entsprechenden Adapter (14) angebracht ist und die Ventile (6) ringförmig um den Mischerblock (10) herum angeordnet sind. The invention relates to a mixer system (1) for a liquid chromatography system, having a mixer block (10), a plurality of fluid inlets (2) which are each connected to a mixing chamber (4) by a channel (3), and a fluid outlet (5). for mixed fluid, each channel (3) being associated with a valve (6) which can control the fluid flow, characterized in that an adapter (14) which is attached to the mixer block (10) is provided for each valve (6). and through which one of the channels (3) passes, the valve (6) being attached to the corresponding adapter (14), the valves (6) being arranged annularly around the mixer block (10).

A biosensor coated with electroactive polymer layer for extension of biosensor life span

본 발명은 전기감응성 고분자층이 부착된 바이오센서에 관한 것으로서, 보다 구체적으로는 바이오리셉터의 표면에 부착된 전기감응성 고분자층 및 상기 전기감응성 고분자층에 연결된 전극을 포함하는 바이오센서로서, 상기 전극에 전기자극을 제공하면 전기감응성 고분자층이 가역적인 변형을 일으킬 수 있고, 이에 의해 바이오리셉터의 표면이 애널라이트에 노출되어 애널라이트의 농도 분석이 가능하게 되는 바이오센서에 관한 것이다. The present invention relates to a biosensor with an electrically sensitive polymer layer, and more particularly, to a biosensor comprising an electrically sensitive polymer layer attached to a surface of a bioreceptor and an electrode connected to the electrically sensitive polymer layer. Providing an electrical stimulation relates to a biosensor that can cause a reversible deformation of an electrically sensitive polymer layer, thereby exposing the surface of the bioreceptor to an analyte and allowing analysis of the analyte concentration. 본 발명에 따른 바이오센서는 이식형 바이오센서로서 이용되었을 때 바이오리셉터가 애널라이트에 노출되는 시간과 빈도가 조절될 수 있어 바이오센서의 수명이 탁월하게 연장되는 장점이 있다. When the biosensor according to the present invention is used as an implantable biosensor, the time and frequency at which the bioreceptor is exposed to the analyte can be controlled, so that the lifespan of the biosensor is prolonged. 이식형 바이오센서, 수축이완, 전기감응성 폴리머, 인공근육, 하이드로겔 Implantable Biosensor, Relaxation Relaxation, Electric Sensitive Polymer, Artificial Muscle, Hydrogel

Affinity chromatography carrier

本发明提供一种杂质的非特异吸附得到抑制、且因长期保存所致的动态结合容量的降低得到抑制的保存稳定性优异的亲和色谱用载体。所述亲和色谱用载体包含固相载体、使环氧基开环而得到的开环环氧基和结合于所述固相载体的配体，所述固相载体含有包含(M－1)结构单元和(M－2)结构单元的共聚物，(M－1)结构单元是来自含环氧基的单乙烯基单体的结构单元，相对于全部结构单元为大于20质量份且为99.5质量份以下，(M－2)结构单元是来自聚乙烯基单体的结构单元，相对于全部结构单元为0.5～80质量份，其中，将所述亲和色谱用载体填充于任意的柱容器，用含有2M的NaCl的pH7.5的20mM磷酸钠缓冲液对柱进行置换，在40℃下静置7天时的柱内的pH上升幅度为2以下。

Equipment for performing chromatography

ANOMALY DETECTION AND DIAGNOSIS IN CHROMATOGRAPHY APPLICATIONS

A method for anomaly detection and diagnosis in a chromatography system including a sample handling unit, a chromatographic separation unit, and a detection unit is disclosed. The method can include performing, with the chromatography system, a chromatography analysis of a sample to obtain a chromatogram of the sample, the sample including a known quantity of a reference standard. The method can also include determining peak information corresponding to the reference standard in the chromatogram and determining whether the peak information conforms with an expected response of the chromatography system associated with the reference standard. If the peak information does not conform with the expected response, the method can include determining that there is an anomaly in the operation of the chromatography system and diagnosing a cause of the anomaly as relating to the operation of at least one the sample handling unit, the chromatographic separation unit, and the detection unit.

Method for producing unsaturated uronic acid monosaccharide using alginate lyase and the enzyme

The purification process of antibody

根据一实施方式而提供一种方法，其为抗体的纯化方法，包括使包含抗体及HCP的溶液与色谱法载体接触而分离所述抗体与所述HCP，所述色谱法载体包括包含多孔性粒子的基础载体、以及与所述基础载体键结的具有多个一级氨基的化合物，所述具有多个一级氨基的化合物中的一级氨基的20％～55％由疏水基修饰，所述抗体的回收率为85％以上，纯化后的抗体溶液中的所述HCP量小于45ppm。

Device for recycling the carrier gas of a gas phase chromatograph

L’invention propose une méthode d’opération d’une installation d’analyse de gaz par chromatographie  en phase gazeuse, installation qui comporte  les éléments suivants : un chromatographe  (28) en phase gazeuse ;un détecteur (26), par exemple  un détecteur  à conductibilité thermique ;une source de gaz porteur  (16); du type où le gaz porteur balaie en continu la colonne de séparation (20) du chromatographe et le détecteur,  méthode organisant le recyclage du gaz porteur. Figure de l’abrégé : Fig. 1 The invention proposes a method of operating a gas analysis installation by gas phase chromatography, installation which comprises the following elements: a gas phase chromatograph (28); a detector (26), for example a detector thermally conductive; a source of carrier gas (16); of the type where the carrier gas continuously sweeps the separation column (20) of the chromatograph and the detector, a method organizing the recycling of the carrier gas. Abstract Figure: Fig. 1

Method of controlling fluid flow in microfluidic device and microfluidic analysis apparatus

본 발명은 미세유체 소자의 유체 흐름 조절 방법 및 미세유체분석장치에 관한 것으로, 이 방법은 수평에 대한 미세유체소자의 기울기를 조절함으로써, 미세유체소자 내에서 간단하고 정밀하게 유체의 흐름을 제어할 수 있다. 이로써 유체의 이송을 완벽하게 제어할 수 있으며, 적은 양의 시료만으로 검사를 진행할 수 있다. 이 방법은 사용자에 의해 수동으로 진행될 수 있어 전력을 필요로 하지 않아 경제적이며, 간단하다. 또한 이 미세유체 분석 장치는 수평에 대해 상기 미세유체소자 수용부의 기울기를 유발하기 위한 기울기 작동수단과 상기 기울기 작동수단의 동작 상태를 제어하기 위한 기울기 제어부를 구비함으로써, 간단하고 정밀하게 유체의 흐름을 제어하여 유체를 정확하게 분석할 수 있다. The present invention relates to a method for controlling a fluid flow of a microfluidic device and a microfluidic analysis device. The method can control the flow of a fluid in a microfluidic device simply and precisely by adjusting the inclination of the microfluidic device with respect to the horizontal. Can be. This allows complete control of the flow of fluid and allows inspection with only a small amount of sample. This method is economical and simple because it can be performed manually by the user, requiring no power. In addition, the microfluidic analysis device includes a tilt operation means for causing the tilt of the microfluidic device accommodating portion with respect to the horizontal and a tilt control unit for controlling the operation state of the tilt operation means, thereby providing a simple and precise flow of fluid. Controlled for accurate fluid analysis 미세유체소자, 미세유체분석장치, 기울기, 중력, 모세관력 Microfluidic device, microfluidic analysis device, slope, gravity, capillary force

Apparatus and methods for preparative liquid chromatography

A method of liquid chromatography includes providing one or more solvent reservoirs, providing a solvent pump, drawing one or more solvents into the pump in response to a pressure drop that promotes outgassing of the solvents, and dispersing outgassed bubbles into smaller bubbles to promote re-dissolution of the gas. A liquid-chromatography apparatus includes at least two solvent reservoirs, a pump, at least one bubble-dispersing unit that receives a pressurized flow of proportioned solvents from the pump, and a control unit. The control unit includes a processor and a memory that stores instructions; the control unit controls proportioning of solvents to obtain a preselected solvent composition, and pumping at flow rates to support preparative-scale or process-scale liquid chromatography.

Chromatograph and sample analysis method

Ion chromatography systems with flow-delay eluent recycle

A chromatographic method including chromatographically separating sample ionic species in an eluent stream, detecting the separated sample ionic species, catalytically combining hydrogen and oxygen gases or catalytically decomposing hydrogen peroxide in a catalytic gas elimination chamber (31), and recycling the effluent stream from the catalytic gas elimination chamber to the chromatography separation column (10). The residence time between the detector (14) and said chamber (31) is at least one minute to facilitate decomposition of unstable oxidative compounds. Also, flowing the recycle sequentially through two detector effluent flow channels of an electrolytic membrane suppressor (28). Also, applying heat or UV energy between the detector (14) and the chamber (31). Also, detecting bubbles after the chamber. Also, a Platinum group metal catalyst and ion exchange medium in the chamber. Apparatus for performing the methods.

Liquid feeder

Filler for hydrophilic interaction chromatography

Directed-flow assay device

A diagnostic assay device that directs an applied sample to the analytical membrane of a directed flow device. The device has a sample receiving port defined by layers of built-up material on one end of the test strip. The port contains the sample and specifically directs it to the membrane in a controlled fashion. Additional features include configuration of the housing in a general C-shape with the test strip spanning the opening of the C-shape to allow access by a reader device. See Figure 5. A preferred method employs superparamagnetic particles to label the target analytes for detection and measurement by means of an electromagnetic reader device.

Liquid monitoring device for analysis

本发明提供一种分析用液体监视装置，能够在分析用液体的设置、配管连接存在错误时将该情况检测为异常。分析用液体监视装置具备：重量计，其测量容纳有作为分析中应该使用的分析用液体的液体的容器的重量；密度存储部，其存储所述分析用液体的密度ρ；以及分析用液体判定部，其构成为基于所述重量计的测量值来求出使所述分析用液体减少的减少动作前后的所述容器的重量的变化量ΔM，并基于所求出的所述变化量ΔM、所述减少动作中的所述容器内的液体减少的体积V、以及存储于所述密度存储部的所述分析用液体的密度ρ，来判定在所述减少动作中是否正常地进行了所述分析用液体的使用。

Protein chromatography system

Directed-flow assay device

一种将施加的样品导向一个定向流装置的分析膜的诊断化验装置。该装置在测试条的一端具有由多层拼装的材料所限定的一个样品接收端口，该端口容纳该样品，并以一种控制的方式特效地把样品导向该分析膜。另外的特征包括：大致C形的外壳结构，该测试条跨接该C形的开口，以便允许一个读数装置访问。一种优选的方法采用超顺磁性微粒来标记目标分析物，以通过一个电磁读取装置进行检测和测定。

Check valve with polymer seat and poppet valve

Because illusion caused by the different qualities of fluid containment volume in sample separation equipment compensates

一种用于控制对流体样品进行分离的样品分离设备(100)的至少一部分的控制装置(70)，该样品分离设备(100)包括至少两个流体容纳体积(102、104)，该至少两个流体容纳体积(102、104)具有不同的流通特性并且每个流体容纳体积构造成临时容纳流体样品，其中，该控制装置(70)构造成控制样品分离设备(100)的至少一部分的操作，以用于至少部分地补偿由至少两个流体容纳体积(102、104)的不同流通特性所导致的样品分离假象。

Liquid chromatography device and liquid chromatography

本发明提供一种液相层析装置(A)，其包括：试样制作单元(1)；分离试样的成分的柱(52)；具有将洗脱液(La、Lb)供给到柱(52)的送出单元(3)的洗脱液送出单元；能够将一定量的上述试样和洗脱液(La、Lb)导入柱(52)的流路切换阀(2)；对由柱(52)分离的上述试样的成分和上述洗脱液(La)构成的被检测液进行分析的分析单元；和控制单元(6)。其中，上述洗脱液供给单元，以非混合状态将洗脱液(La、Lb)供给到流路切换阀(2)。通过这样的结构，能够实现分析时间的缩短和洗脱液消耗的减少。

Connector with structural reinforcement and biocompatible fluid passage

Directed-flow assay device

A diagnostic assay device that directs an applied sample to the analytical membrane of a directed flow device. The device has a sample receiving port defined by layers of built-up material on one end of the test strip. The port contains the sample and specifically directs it to the membrane in a controlled fashion. Additional features include configuration of the housing in a general C-shape with the test strip spanning the opening of the C-shape to allow access by a reader device. See Figure 5. A preferred method employs superparamagnetic particles to label the target analytes for detection and measurement by means of an electromagnetic reader device.

Lcms technology and its uses

The present invention relates to an improved LCMS technology and its uses in methods for the selective identification and characterization of immunogenic pathogen associated epitopes, and the use thereof in vaccine development. One way of bridging the knowledge gap on T cell epitopes is to apply a new platform technology, ''immunoproteomics'', to directly assess the epitope display at the surface of antigen presenting cells by nanoscale mass spectrometry of extracted peptide samples. This is the only methodology that can provide unbiased insight into epitope features such as the exact molecular nature, diversity, abundance, dynamics and PTM of T cell epitopes originating from pathogen-derived proteins. Therefore, this platform technology and immunoproteomics should become an intrinsic part of vaccinology.

Separation analysis method

合成オリゴヌクレオチドを医薬品へ応用するために、オリゴヌクレオチド反応混合物に含まれる不完全長のオリゴヌクレオチドなどの不純物を高度に分離分析する手法を提供する。水溶性有機溶媒と水とを含む溶離液を用いたカラムクロマトグラフィーにより、１９量体以上の合成オリゴヌクレオチドを分離分析する方法である。疎水性官能基が固定された非多孔性の粒子充填剤を含む。

Directed-flow assay device

Protein chromatography system

The invention features an apparatus for the separation and analysis of proteins, which includes sample input means, a first liquid chromatography column, a multiport injection valve connecting the sample input means to the column, pump means for providing variable pressure delivery of a solution to the column via the multiport valve, and program means for specifying a sequence of system control programs.

DNA sequence and expression vector for alginate lyase

Alginate lyase activity is exhibited by the amino acid sequences (polypeptides) shown in SEQ ID No:1 and SEQ ID No:2. These polypeptides and their homology equivalents are used to produce 4-deoxy-L-erythro-5-hexoseulose uronic acid (DEH) by contacting the polypeptide(s) with an alginate containing a uronic acid moiety and holding the mixture of the alginate and the polypeptide(s) at a temperature at which alginate lyase activity is exhibited.

Artifact compensation due to different properties of fluid accommodation volumes in sample separation apparatus

A control device 70 for controlling at least part of a sample separation apparatus 100 for separating a fluidic sample, the sample separation apparatus 100 comprising at least two fluid accommodation volumes or sample loops 102, 104 having different flow through properties and each being configured for temporarily accommodating fluidic sample, wherein the control device 70 is configured for controlling operation of at least part of the sample separation apparatus 100 for at least partially compensating for sample separation artifacts resulting from the different flow through properties of the at least two sample loops 102, 104. The control device compensates for separation artifacts in a multiple sample loop system, in particular a dual loop system, by allowing partial fluid flow through the sample loops, adjusting the timing of the start and/or end of the introduction of a fluidic sample from the sample loops, adjusting the timing of the start and/or end of a mobile phase profile, adjusting the timing of switching a fluidic switch between the sample loops, or adjusting the mobile phase gradient.

Method for producing conjugated-diene-based copolymer latex

A method for producing a conjugated-diene-based copolymer latex, comprising a step of performing the emulsion polymerization of a monomer component containing 10 to 80% by mass of an aliphatic conjugated-diene-based monomer, 0.5 to 15% by mass of an ethylenically unsaturated carboxylic acid monomer and 5 to 89.5% by mass of another copolymerizable monomer, wherein the ethylenically unsaturated carboxylic acid monomer comprises itaconic acid which does not show a peak that is detected in a time range between a detection time of 12 minutes and a detection time of 14 minutes in a chromatogram measured under the below-mentioned measurement conditions. <Measurement conditions> The measurement is carried out using a liquid chromatography-corona charged aerosol detector, wherein the concentration of a sample is 1% by mass, the solvent is pure water, the column temperature is 40°C, the mobile phase is an aqueous formic acid solution/acetonitrile, and the feed rate is 0.2 ml/min.

Mixers and liquid chromatographs used in liquid chromatographs

液体クロマトグラフで使用されるミキサは、複数種類の溶離液が流入する流入口と、流入口から流入した複数種類の溶離液を混合する混合部と、混合部において混合された複数種類の溶離液が流出する流出口であって、分離カラムに至る流路に接続される流出口と、流入口および流出口の両方、あるいは、流出口に設けられたミキサフィルタとを備え、ミキサフィルタは、バインダを用いない金属材料の焼結体で構成される。 The mixer used in the liquid chromatograph consists of an inlet in which multiple types of eluents flow in, a mixing section in which multiple types of eluents flowing in from the inlet are mixed, and a plurality of types of eluents mixed in the mixing section. The mixer filter includes an outlet connected to a flow path leading to a separation column, both an inlet and an outlet, or a mixer filter provided at the outlet. It is composed of a sintered body of a metal material that does not use.

Detection method and application of related substances of pezopanib hydrochloride intermediate PZP-M1

本发明涉及化学药物分析方法技术领域，尤其是涉及一种盐酸培唑帕尼中间体PZP‑M1的有关物质的检测方法和应用。检测方法，包括如下步骤：采用高效液相色谱对供试品溶液进行检测；所述高效液相色谱的检测条件包括：色谱柱为十八烷基硅烷键合硅胶色谱柱；检测波长为213～217nm；以pH为3.0～3.2的0.009～0.011mol/L磷酸二氢钾的水溶液作为流动相A、甲醇作为流动相B进行梯度洗脱。通过本发明的高效液相色谱检测条件，可以实现一次性高效的分离出上述8种有关物质，在保证各有关物质与有效成分高效分离的基础上，使其有良好的专属性、重复性与准确度等。

Gas chromatograph

在气相色谱仪(1)中，背压算出处理部(242)计算出第1检测器的背压。压力算出处理部(243)计算出分支部中的载气的压力。逆流判定处理部(244)将压力算出处理部(243)所计算出的分支部中的载气的压力与背压算出处理部(242)所计算出的第1检测器的背压进行比较，在分支部中的载气的压力比第1检测器的背压小的情况下，判定为载气逆流这一主旨。因此，通过确认逆流判定处理部(244)的判定结果，能够在有可能发生载气的逆流的情况下，可靠地认识到该情况。

Facility for executing chromatographic process

Method for separating steviol glycoside, method for producing rebaudioside a and device for separating steviol glycoside

A method for separating steviol glycoside(s), said method involving separation step 55 for performing continuous liquid chromatography wherein a liquid to be separated, which contains multiple kinds of steviol glycosides, is passed through a separating agent comprising polyethylene imine fixed to a support to thereby continuously separate at least one kind of steviol glycoside.

Liquid chromatography device and liquid chromatography

【課題】分析時間の短縮および溶離液消費の減少を図ることが可能な液体クロマトグラフィ装置および液体クロマトグラフィを提供すること。 【解決手段】試料作製手段１と、試料の成分を分離するカラム５２と、溶離液Ｌａ，Ｌｂをカラム５２に供給する送出手段３を含む溶離液送出手段と、カラム５２に一定量の上記試料と溶離液Ｌａ，Ｌｂとを導入可能である、流路切替バルブ２と、カラム５２によって分離された上記試料の成分と上記溶離液Ｌａとからなる被検液の分析を行う分析手段と、制御手段６と、を備えた液体クロマトグラフィ装置Ａであって、上記溶離液供給手段は、溶離液Ｌａ，Ｌｂを非混合状態で、流路切替バルブ２に供給する。 【選択図】 図１

Liquid feeding device and liquid chromatograph

This liquid feeding device is provided with: a liquid feeding pump which suctions and feeds a mobile phase from a mobile phase container through a suction pipe having an end portion dipped in the mobile phase in the mobile phase container for accommodating the liquid mobile phase; and at least one dissolved material removal filter filled with a filler having a property of adsorbing dissolved material in the mobile phase.

Chromatograph system, autosampler and cleaning method

Methods for analyzing sugars

Electrolytic eluent generator and method of use

Directed-flow assay device

Transmission filter for calibrating a wavelength selecting selector

Optikvorrichtung (100), aufweisend eine polychromatische elektromagnetische Strahlungsquelle (102) zum Emittieren polychromatischer elektromagnetischer Strahlung, eine Auswähleinrichtung (104) zum Auswählen eines Wellenlängenteilbereichs der polychromatischen elektromagnetischen Strahlung, und eine Kalibriereinrichtung (106) mit einem Transmissionsfilter (108) zum Kalibrieren der Auswähleinrichtung (104). Optical device (100) comprising a polychromatic electromagnetic radiation source (102) for emitting polychromatic electromagnetic radiation, selection means (104) for selecting a wavelength subset of said polychromatic electromagnetic radiation, and calibration means (106) having a transmission filter (108) for calibrating said selection means (10) 104).

Mobile phase monitor, liquid chromatograph, analysis system, and program

A mobile phase monitor includes a measurement unit, an arithmetic unit, a storage, and a discrimination unit. The measurement unit measures a weight of a mobile phase container. The arithmetic unit produces a calibration curve indicating a relationship between a measurement value of the measurement unit and a liquid amount of the mobile phase accommodated in the mobile phase container. The arithmetic unit calculates the liquid amount of the mobile phase from the measurement value of the measurement unit based on the produced calibration curve. The storage stores a plurality of calibration curves respectively corresponding to a plurality of types of mobile phases. The discrimination unit discriminates a type of the mobile phase accommodated in the mobile phase container by searching for the produced calibration curve from the plurality of calibration curves stored in the storage.

Patent JPWO2021250948A1

Analyzing device

A liquid chromatography device A includes a column 1 containing a filler, an injection valve 4 capable of introducing a sample into the column 1 and also capable of introducing a liquid mobile phase into the column 1, and a mobile phase feeder 3 for feeding the liquid mobile phase from a mobile phase container B containing the liquid mobile phase to the column 1 via the injection valve 4. Between the mobile phase container B and the injection valve 4 is provided a storage chamber 5 for temporarily storing the liquid mobile phase sent from the mobile phase container B. The device further includes a liquid level detection sensor for detecting the liquid level of the liquid mobile phase in the storage chamber 5. This structure allows the liquid for use in analysis to be used completely without being wasted.