СПОСОБЫ ДИАГНОСТИКИ ХРОНИЧЕСКОГО ЗАБОЛЕВАНИЯ КЛАПАНОВ

Изобретение относится к ветеринарии и может быть использовано для диагностики хронического дегенеративного заболевания клапанов сердца у животного семейства псовых. Для этого получают биологический образец животного и анализируют его на присутствие метаболита глутамата и, при необходимости, другого метаболита, связанного с хроническим заболеванием клапанов. Затем сравнивают количество каждого метаболита, идентифицированного в образце, с количеством того же самого метаболита, присутствующего в образцах сравниваемых контрольных животных, не страдающих хроническим заболеванием клапанов. Если количество каждого такого метаболита, найденного в образце животного семейства псовых, больше количества, присутствующего в образце контрольного животного семейства псовых, то указанное сравнение применяют для диагностики хронического заболевания клапанов у животного. Изобретение обеспечивает способ диагностики хронического заболевания клапанов у собак. 8 з.п. ф-лы, 1 табл., 1 пр.

УСТРОЙСТВО РАЗДЕЛЕНИЯ ФАЗ С РАЗНЫМИ ПЛОТНОСТЯМИ

Группа изобретений относится к устройствам для взятия и транспортировки проб текучей среды, в процессе которой устройства и пробу текучей среды подвергают центрифугированию для отделения фракций с более высокой плотностью от фракции с более низкой плотностью. Механический разделитель для разделения пробы жидкости на первую и вторую фазы внутри контейнера для пробы имеет корпус разделителя со сформированным в нем сквозным каналом, обеспечивающим прохождение через него жидкости. Корпус разделителя содержит поплавок, имеющий первую плотность, и балласт, имеющий вторую плотность, превышающую первую плотность, причем часть поплавка соединена с частью балласта. Узел разделителя для обеспечения разделения пробы текучей среды на первую и вторую фазы содержит контейнер для пробы, имеющий первый конец, второй конец и боковую стенку, проходящую между ними, при этом продольная ось контейнера для пробы проходит между первым и вторым концами, и механический разделитель. Механический разделитель содержит ...

СПОСОБ ПРОГНОЗИРОВАНИЯ ВЕРОЯТНОСТИ ВЫЯВЛЕНИЯ РАКА ШЕЙКИ МАТКИ НА ОСНОВЕ РУТИННЫХ ПОКАЗАТЕЛЕЙ КРОВИ

Изобретение относится к медицине, а именно к гинекологии и онкологии, и может быть использовано для прогнозирования вероятности выявления рака шейки матки (РШМ). Проводят определение показателей в сыворотке крови. Определяют следующие показатели: Возраст, Фибриноген, Агрегация тромбоцитов с АДФ, АЧТВ, СОЭ, Гемоглобин, Эритроциты, Лейкоциты, Средний объем эритроцита, Щелочная фосфатаза, СРБ, Сывороточное железо, Насыщение трансферрина железом, Креатинин, АСТ, Трансферрин. С учетом полученных данных рассчитывают прогностический коэффициент по формуле: ВПР = 1/(1 + e-x), где: ВПР - вероятность прогнозирования результата – наличия рака шейки матки, е - основание натурального логарифма. x = + 0,663×(Возраст) + 3,786×(Фибриноген) + 0,904×(АЧТВ) + 0,368×(Насыщение трансферрина железом) + 0,254×(СОЭ) + 0,180×(Щелочная фосфатаза) + 0,137×(СРБ) – 0,116×(Сывороточное железо) – 0,155×(Креатинин) – 0,195×(Ср объем эритроцита) – 0,195×(Агрегация тромбоцитов с АДФ) – 0,231×(АСТ) – 0,378×(Трансферрин) ...

Способ диагностики травматической дисфункции эндотелия при сочетанной шокогенной травме

Изобретение относится к медицине, а именно к хирургии и судебно-медицинской экспертизе, и может быть использовано для диагностики травматической дисфункции эндотелия при сочетанной шокогенной травме. На ранних этапах оказания медицинской помощи проводят сочетанный анализ биомеханики травматического воздействия клинического случая, вычленяя категории пострадавших с наличием сформированных условий гидродинамического удара в аорте в момент травмы: категория пострадавших от высокоэтажного падения - выше 3-го этажа, с первичным соударением тазово-поясничной, ягодичной областью, категория пострадавших - водитель и пассажир переднего сиденья, в первую очередь фиксированных ремнем безопасности, при дорожно-транспортных происшествиях при скорости движения автомобиля не менее 40 км/ч, категория пострадавших - пешеход, наезд автомобиля, с первичной областью приложения травмирующей силы в области бедер и ягодиц с последующим забрасыванием тела на капот автомобиля, при дорожно-транспортных происшествиях ...

Способ прогнозирования рецидива язвенного гастродуоденального кровотечения

Изобретение относится к медицине, а именно к абдоминальной хирургии и гастроэнтерологии, и может быть использовано для прогноза риска рецидива язвенного кровотечения. Определяют шоковый индекс (ШИ), количество эритроцитов, общего белка, мочевины, локализацию язвы и тип гемостаза по Forrest. Каждый признак оценивают в баллах. ШИ 1,0 или более оценивают как 1 балл. Количество эритроцитов менее 3,0*1012 оценивают как 1 балл; общего белка 58 г/л или менее оценивают как 4 балла; мочевины более 8 ммоль/л оценивают как 4 балла. Локализацию язвы по малой кривизне желудка оценивают как 2 балла. Локализацию по задней стенке луковицы двенадцатиперстной кишки - как 3 балла. Состояние дна язвы Forrest 2А оценивают как 2 балла. Состояние дна язвы Forrest 2С или 3 - как минус 3 балла. Осуществляют суммирование баллов, при этом дополнительно определяют количество тромбоцитов и активированное частичное тромбопластиновое время (АЧТВ). Количество тромбоцитов менее 150*109 оценивают как 2 балла; АЧТВ 40 секунд ...

СПОСОБ ОПРЕДЕЛЕНИЯ СОДЕРЖАНИЯ ГОМОАРГИНИНА В ПЛАЗМЕ КРОВИ И ДРУГИХ БИОЛОГИЧЕСКИХ ЖИДКОСТЯХ ЧЕЛОВЕКА

Изобретение относится к медицине, а именно к клинической лабораторной диагностике, и может быть использовано для определения гомоаргинина (гАрг) в плазме крови и других биологических жидкостях человека. На этапе пробоподготовки добавление к биологическому образцу внутреннего стандарта выполняют одновременно с депротеинизацией путем осаждения 0,2% раствором муравьиной кислоты в метаноле, содержащем внутренний стандарт норвалин, при этом образец и реагент берут в объемном соотношении 1:1-1:2, затем выполняют разделение и детекцию гомоаргинина путем высокоэффективной жидкостной хроматографии с предколоночной дериватизацией ортофталевым альдегидом и флюориметрическим детектированием и определение концентрации гомоаргинина по полученной хроматограмме. Способ позволяет избежать дорогостоящих расходных материалов, необходимых для проведения твердофазной экстракции, и отличается меньшей трудоемкостью пробоподготовки при сопоставимых аналитических характеристиках. 2 ил., 3 табл., 1 пр.

Способ оценки риска летального исхода у пациентов с новой коронавирусной инфекцией

Изобретение относится к медицине, а именно к сосудистой хирургии, и может быть использовано для оценки риска летального исхода у пациентов с новой коронавирусной инфекцией (НКИ). Осуществляют определение уровня МСР-1 и тромбоцитов, определение наличия ИБС и СД 2 типа, учет приема перорального антикоагулянта (ПОАК) и оценку степени тяжести НКИ. Вероятность развития летального исхода рассчитывают по формуле: Р = 1 / (1 + е-Z), где Р – вероятность прогнозирования летального исхода, е – основание натурального логарифма - число Эйлера, а Z = -10.108 + 0.009 × X1 - 0.01 × X2 + 1.777 × Х3 + 1.455 × Х4 - 3.975 × Х5 + 3.084 × Х6, где Х1 – исходные значения МСР-1, пг/мл; Х2 – исходные значения тромбоцитов, ×109 /л; Х3 – бинарная переменная, отражающая факт наличия ИБС у пациента: 1 – да, 0 – нет; Х4 – бинарная переменная, отражающая факт наличия СД 2 типа у пациента: 1 – да, 0 – нет; Х5 – бинарная переменная, отражающая факт использования ПОАК у пациента: 1 – да, 0 – нет; Х6 – категориальная переменная ...

СПОСОБ ОПРЕДЕЛЕНИЯ СОДЕРЖАНИЯ ГЕМОГЛОБИНА В ЭРИТРОЦИТАХ И УСТРОЙСТВО ДЛЯ ЕГО ОСУЩЕСТВЛЕНИЯ

Использование: медицина, для определения содержания Hb в эритроцитах. Сущность изобретения: пробу цельной крови помещают в капиллярную трубку с пластмассовым поплавком. Этот поплавок удлинен по оси и имеет удельный вес, который позволяет ему плавать в уплотненном слое красных клеток крови, когда проба центрифугируется в трубке. Концентрация гемоглобина в уплотненных красных клетках крови измеряется путем определения глубины, на которую поплавок погружается в слой красных клеток, и затем рассчитывается его концентрация в крови. Гемоглобин может измеряться таким образом, поскольку фактически единственным компонентом красных клеток, дающим вклад в плотность красных клеток, является гемоглобин. Концентрация гемоглобина в цельной крови расcчитывается путем умножения средней корпускулярной концентрации гемоглобина в уплотненных красных клетках крови на объемный процент уплотненных клеток (гематокрит) в цельной крови. Все необходимые вычисления могут быть осуществлены с помощью предварительно ...

РАЗДЕЛИТЕЛЬНЫЙ ЭЛЕМЕНТ

Изобретение относится к разделительному элементу для отделения первой фазы от второй фазы. Разделительный элемент для отделения сыворотки крови от сгустка крови под действием центробежной силы в трубкообразной емкости, содержащий изготовленное из упругого материала плавучее тело с проходящим на виде сверху по периметру уплотняющим краем для герметизирующего прилегания к внутренней стороне трубкообразного сосуда в уплотнительной позиции и по меньшей мере один закрепленный на нижней стороне плавучего тела балластный элемент, причем плотность балластного элемента больше, чем плотность плавучего тела, и причем плотность всего разделительного элемента лежит в диапазоне значений между плотностью сыворотки крови и плотностью сгустка крови. При этом плавучее тело выполнено дискообразным, и балластный элемент выполнен в форме множества лапок, которые с распределением по его краю проходят вниз от нижней стороны этого дискообразного плавучего тела. Изобретение обеспечивает альтернативный разделительный ...

Способ индивидуальной оценки биосовместимости с организмом имплантируемых полимерных материалов

Изобретение относится к медицине, а именно к иммунологии, имплантологии, хирургии, и может быть использовано для индивидуальной оценки биосовместимости с организмом имплантируемых полимерных материалов. Проводят отбор исследуемого материала у индивидуума. Забор пробы крови у пациента выполняют в предимплантационный период, кровь центрифугируют для получения популяции мононуклеарных лейкоцитов. Полученную клеточную суспензию вносят в лунки планшета с образцами имплантируемых полимерных материалов в виде цилиндров длиной 3 мм и диаметром 1 мм, в количестве 1 штуки на лунку круглодонного планшета, инкубируют при 5% СО2в течение 72 ч при температуре 37°С. Далее определяют количество мононуклеарных лейкоцитов и концентрацию провоспалительных цитокинов: ИЛ-1β и ИЛ-8. Полученные показатели выражают в виде индекса стимуляции, который рассчитывают по формуле: ИС=УП/УС, предварительно рассчитав удельную продукцию цитокина в присутствии полимерного образца и в присутствии стекла по формулам: УП=Ц/ ...

Способ прогнозирования норовирусного гастроэнтерита у детей

Изобретение относится к медицине, а именно к педиатрии и инфекционным болезням, и может быть использовано для диагностики норовирусного гастроэнтерита у детей путем учета анамнеза и клинико-лабораторных данных. В остром периоде в 1 сутки заболевания определяют: характер лихорадки, частоту стула, наличие многократной рвоты, уровень лейкоцитов в клиническом анализе крови с последующим расчетом линейных дискриминантных функций по формулам: ЛДФ1=2,14X1+0,42X2+0,63X3+0,89X4-8,42, ЛДФ2=1,92X1+0,80X2+0,39X3+1,68X4-21,02, где: X1 - выраженность лихорадки в первые сутки болезни (1 - субфебрильная: 37,0-37,9°С; 2 - фебрильная: 38,0-38,9°С; 3 - высокая фебрильная: 39,0-39,9°С; 4 - гиперпиретическая: 40,0°С и более); Х2 - частота стула в первые сутки болезни; Х3 - количество эпизодов рвоты в первые сутки болезни; Х4 - уровень лейкоцитов в гемограмме в 1 сутки болезни. Сравнивают значения показателей ЛДФ1 и ЛДФ2 и при ЛДФ2 > ЛДФ1 диагностируют наличие норовирусного гастроэнтерита у детей. Способ обеспечивает ...

Способ прогнозирования развития поздней анемии недоношенных

Изобретение относится к медицине, а именно к неонатологии, педиатрии и гематологии, и может быть использовано для прогнозирования развития поздней анемии недоношенных у глубоконедоношенных детей, родившихся с массой тела менее 1500 г. Проводят определение в периферической венозной крови новорожденного на 3-5 сутки жизни абсолютного числа ретикулоцитов (RET#), среднего объема ретикулоцитов (MCVr), коэффициента насыщения трансферрина железом, содержания ферритина и сывороточного железа. Осуществляют расчет прогностического индекса D по формуле D=6,071-0,0055×А1+0,0607×А2+0,0033×A3-0,7626×А4+0,2325×А5, где А1 - абсолютное число ретикулоцитов (RET#), ×109/л; А2 - средний объем ретикулоцитов (MCVr), fl; A3 - ферритин, мкг/л; А4 - сывороточное железо, мкмоль/л; А5 - коэффициент насыщения трансферрина железом, %, 6,071 – CONSTANT. При D более 0 прогнозируют развитие в три месяца жизни поздней анемии недоношенных. При D менее 0 прогнозируют отсутствие развития в три месяца жизни поздней анемии ...

Способ прогнозирования риска длительного послеоперационного синовита коленного сустава

Изобретение относится к медицине, в частности к травматологии и ортопедии, и может быть использовано для прогнозирования риска длительного послеоперационного синовита коленного сустава у пациентов после артроскопии. Определяют наличие травмы в анамнезе, пол пациента, стадию остеоартрита, длительность синовита до артроскопии в месяцах, уровень СОЭ в мм/ч и наличие или отсутствие проведения физиотерапевтического лечения после артроскопии. С учетом полученных данных определяют прогнозируемую длительность синовита коленного сустава после артроскопии (Y) по заданной формуле. И при значении Y более 2,0 прогнозируют высокий риск сохранения синовита более 2 месяцев. Способ позволяет достоверно определять риск развития послеоперационного синовита коленного сустава после артроскопического лечения и провести своевременную профилактику указанного состояния за счет оценки совокупности наиболее значимых показателей. 2 табл., 3 пр.

Способ прогнозирования успешности обучения студентов в медицинском вузе

Изобретение относится к медицине, а именно к медицинскому образованию, и может быть использовано для прогнозирования успешности обучения студентов в медицинском вузе. В качестве прогностического критерия используют концентрацию тромбоцитов периферической крови по данным общего анализа крови. Если она составляет от включительно 280×109/л до включительно 433×109/л, то прогнозируют высокую успешность обучения. Если концентрация тромбоцитов от включительно 160×109/л до 280×109/л, то прогнозируют более низкую успешность обучения. Способ обеспечивает возможность повышения точности прогноза успешности обучения студентов в медицинском вузе за счет оценки показателя общего анализа крови - концентрации тромбоцитов. 1 ил., 1 табл., 7 пр.

Способ оценки состояния акватории

УСТРОЙСТВО ДЛЯ ОТДЕЛЕНИЯ ПЛАЗМЫ КРОВИ

... 1. Устройство (1) для забора крови (19) и отделения ее составных частей, таких как плазма, в качестве анализируемой жидкости (2), имеющее подводящее устройство (13) для забора крови (2), мембрану (15) для отделения от крови ее составных частей в качестве анализируемой жидкости (2), канал (3), в который анализируемая жидкость (2) поступает и по которому она движется под действием капиллярных сил, и питающее устройство для заполнения канала (3) анализируемой жидкостью (2) на его впускном или входном участке (18), отличающееся тем, что мембрана (15) и/или дно входного участка выполнены/выполнена, соответственно выполнено изогнутой, прежде всего выпуклой, формы и вершиной своей изогнутой части выступают/выступает в питающее устройство в направлении заполнения, при этом мембрана (15) в своей центральной части прилегает ко дну питающего устройства, вследствие чего выходящая с нижней стороны мембраны анализируемая жидкость непосредственно смачивает дно питающего устройства. ! 2. Устройство по ...

УСТРОЙСТВО ДЛЯ РАЗДЕЛЕНИЯ ТЕКУЧЕЙ СРЕДЫ НА ФАЗЫ С РАЗНЫМИ ПЛОТНОСТЯМИ (ВАРИАНТЫ)

... 1. Устройство для разделения пробы текучей среды внутри контейнера для пробы, включающее разделитель, в котором сформирован проходящий вдоль оси сквозной канал, выполненный с возможностью прохождения через него текучей среды, и при этом разделитель содержит:поплавок, имеющий первую плотность; ибалласт, имеющий вторую плотность, превышающую первую плотность, причем часть поплавка соединена с частью балласта,при этом разделитель имеет центр масс и центр объема, смещенный относительно центра масс, ипоплавок формирует верхнюю внешнюю поверхность разделителя, балласт формирует нижнюю внешнюю поверхность разделителя, а ось сквозного канала расположена в плоскости, проходящей под углом относительно плоскости, простирающейся вертикально от высшей точки верхней внешней поверхности поплавка до низшей точки нижней внешней поверхности балласта и через центр разделителя.2. Устройство по п. 1, в котором центр масс смещен в сторону от оси сквозного канала.3. Устройство по п. 2, в котором центр объема ...

Способ прогноза хронической плацентарной недостаточности после перенесённой новой коронавирусной инфекции (НКВИ) лёгкой и среднетяжёлой формы

Изобретение относится к медицине, а именно к акушерству и гинекологии, и может быть использовано для прогноза хронической плацентарной недостаточности у беременных после перенесённой новой коронавирусной инфекции (НКВИ) лёгкой и среднетяжёлой формы. Проводят изучение анамнестических данных: первородящая или повторнородящая, наличие или отсутствие ожирения, наличие или отсутствие гипертонической болезни, наличие или отсутствие варикозного расширения вен нижних конечностей. Проводят забор крови в момент заболевания НКВИ, в образцах сыворотки крови пациенток определяют: уровень тромбоцитов, фибриногена, тромбиновое время, активированное частичное тромбопластиновое время, протромбиновый индекс, международное нормализованное отношение. На основании оценки анамнестических и лабораторных данных вычисляют диагностический индекс DI по формуле: где: – подтверждённое заболевание НКВИ лёгкой и среднетяжёлой формы, 0 – заболевание в первом триместре - 5-12,6 недель или в третьем триместре - 28,0-42 ...

СПОСОБ ПРОГНОЗИРОВАНИЯ ОСЛОЖНЕНИЯ ПОСЛЕ COVID-19 У ЛИЦ С КОМОРБИДНЫМ ФОНОМ В АРКТИЧЕСКОМ РЕГИОНЕ

Изобретение относится к медицине, а именно к патофизиологии, иммунологии, лабораторной диагностике, и может быть использовано для прогнозирования осложнения после COVID-19 у лиц с коморбидным фоном - заболеваниями бронхолегочной системы в арктическом регионе. Осуществляют забор крови из вены. В периферической крови у пациентов в первые сутки с момента выявления COVID-19 определяют общее содержание лейкоцитов и содержание цитокина интерлейкина-10 (ИЛ-10). При низком содержании лейкоцитов менее 4,00±0,01⋅109 кл/л и одновременно высоком содержании ИЛ-10 более 10,00±0,01 пг/мл прогнозируют высокую вероятность риска развития осложнения. Способ обеспечивает возможность проводить быструю скрининговую диагностику, сократить время и упростить методику прогнозирования риска формирования осложнения после перенесенного COVID-19 в первые сутки с момента постановки диагноза у лиц с сопутствующими заболеваниями бронхолегочной системы в арктическом регионе, за счет определения в крови пациентов общего ...

СПОСОБ ОПРЕДЕЛЕНИЯ В-КЛЕТОЧНОЙ НЕХОДЖКИНСКОЙ ЛИМФОМЫ

Настоящее изобретение относится к области биотехнологии, медицины, онкологии, гематологии, иммунологии. Способ определения неходжкинской лимфомы, включающий забор у пациента образца периферической крови, подсчет количества лейкоцитов и доли лимфоцитов в образце периферической крови, оценку доли популяции лимфоцитов HLADR+, лимфоцитов CD3+, В-лимфоцитов CD19+, Т-хелперов CD4+, Т-цитотоксических лимфоцитов CD8+, активированных Т-клеток CD3+HLADR+, NK-клеток CD16+ и оценку иммунорегуляторного индекса CD4+/CD8+ в образце периферической крови. 2 з.п. ф-лы, 2 ил., 1 табл., 4 пр.

Способ прогнозирования риска летального исхода острого инфаркта миокарда у больных сахарным диабетом 2 типа на госпитальном этапе

Изобретение относится к медицине, а именно к кардиологии и эндокринологии, и может быть использовано для прогнозирования риска летального исхода острого инфаркта миокарда у больных сахарным диабетом 2 типа на госпитальном этапе. Проводят забор и клинический анализ показателей периферической крови и уровня высокочувствительного С-реактивного белка (вчСРБ) больного. Устанавливают индекс массы тела (ИМТ), кг/м2, учитывают количество имплантированных коронарных стентов (Nст). Определяют клеточный состав крови больного на третьи сутки после проведения первичного чрескожного коронарного вмешательства: количество нейтрофилов, ×109/л, количество лимфоцитов, ×109/л, количество тромбоцитов, ×109/л, количество моноцитов, ×109/л. Рассчитывают клеточные индексы системного воспаления - нейтрофильно-лимфоцитарное отношение (НЛО), индекс системного иммунного воспаления (SII), индекс системного иммунно-воспалительного ответа (SIRI). Определяют уровень высокочувствительно С-реактивного белка в крови при ...

Способ определения относительного объема частиц в жидкости и устройство для его осуществления

GERÄT, DAS VAKUUM ANWENDET, ZUR AUFTRENNUNG VON KÖRPERFLÜSSIGKEITEN

Vorrichtung zur Blutentnahme

VERFAHREN ZUR TESTUNG VON BLUTKONSERVEN AUF VIRALE VERUNREINIGUNG

KÜVETTE FÜR EINE ANALYSEVORRICHTUNG, VERFAHREN UND DIAGNOSTISCHER TESTSATZ ZUR FESTSTELLUNG VON ANALYTEN IN VOLLBLUTPROBEN

VERBESSERTER APPARAT UND VERFAHREN ZUR TRENNUNG VON ZELLEN

VERFAHREN ZUR KOAGULIERUNG UND TRENNUNG VON BLUT

SUSPENSIONSFLUESSIGKEITSABSCHEIDER

VORRICHTUNG UND VERFAHREN ZUR ABTRENNUNG VON BLUTZELLEN AUS KOERPERFLUESSIGKEITEN, DIE ERYTHROZYTEN ENTHALTEN, UND DEREN VERWENDUNG

FILTERVORRICHTUNG UND VERFAHREN

Verfahren zur Abscheidung von Zellen aus Blut

VORRICHTUNG ZUR ZELLSEPARATION UND DOSIERSPRITZE

Vorrichtung und Verfahren zur Trennung von Bestandteilen einer flüssigen Probe

ÜBERLAUFNACHWEIS VON ROTEN BLUTZELLEN

PISTON CONSTRUCTION

... 1381020 Piston in centrifugal separator container BECTON DICKINSON & CO 28 Dec 1972 [25 April 1972] 04350/74 Divided out of 1381019 Heading B2P The subjectmatter of this Specification is identical with that of the parent Specification but the invention is directed to a piston for use in an apparatus for separating blood into light and heavy components by centrifugation and comprising a body portion having sealing means formed around its periphery, a rigid tubular sleeve mounted in the body portion, and normally closed valve means incorporated in the piston and arranged to open for passing fluid through the piston when there is a fluid pressure differential between the two sides of piston.

APPARATUS FOR THE SEPARATION OF BLOOD

LIQUID SAMPLE COLLECTION TUBE

... 1397162 Container for blood fraction P OHRINGER 24 Aug 1973 [18 Sept 1972] 40254/73 Heading G1B [Also in Division B 1] A collection container for isolating a liquid sample, for example blood serum or plasma, from a cylindrical specimen container comprises elongated cylindrical tube 10 having an aperture at one end, annular disc means 13 formed radially about the aperture for slidably engaging the internal cylindrical wall of the specimen container 10 and capable of flexure towards and away from the tube abutment means 14 formed on the tube adjacent to disc means 13 as shown for engaging and supporting at least a portion of the annular disc means on flexure towards tube 10, i.e. on insertion of the tube into specimen container 20, and fluid resistance means such as foam plastics filter 15 disposed in the aperture to limit fluidflow from the tube. In use, on retraction of tube 10, disc means 13 deflects, Fig. 4, allowing air to flow into the container. Tube 10, which may have graduation marks ...

Apparatus and method for monitoring a sedimentation parameter in a fluid medium sample

Apparatus and method for preparing a biological sample

... 1,101,063. Sorting biological cells photoelectrically. INTERNATIONAL BUSINESS MACHINES CORPORATION. Oct. 17, 1966 [Nov. 17, 1965], No. 46235/66. Heading G1A. Biological cells having a particular characteristic are separated from a sample of cells by passing them along a narrow passageway which allows only one cell to pass at a time detecting which cells have the characteristic and diverting the detected cells to a collecting station. Cells from a cervical smear to be tested for cancer are fed along a capillary tube 3 and tested using the system disclosed in Specification 1, 080, 084. Normally a valve 14 is open and a suction pump draws the sample into chamber 12. When a cancerous cell is detected, valve 14 is closed and 15 is opened to draw the cell into the capillary tube 11. The cancerous cells collect on a slide 20 for examination by a cytologist. The detecting system can give signals indicative of different types of blood cells such as leukocytes, red cells and lymphocytes and these ...

Testing Filter Media

A fibrous filter medium is subjected to grafting of hydroxyethyl, hydroxyethyl methocrylate and carboxylic acid groups thereto, in order to present anionic groups in the fibre surfaces and thereby enhance its ability to remove leucocytes from blood plasma without depleting the platelets. The efficiency of the medium to do this is tested by passing an aqueous solution of cationic dye therethrough. The dye is initially adsorbed from the solution, but eventually the emerging liquid turns from colourless to coloured as the medium saturates. The quantity of solution passed before this happens gives a measure of the efficiency.

THE CONTROL OF GRAFTING PROCESSES IN THE PRODUCTION OF FIBROUS LEUKOCYTE DEPLETION FILTERS

METHOD AND APPARATUS FOR LYMPHOCYTE SEPARATION FROM BLOOD

... 1386303 Magnetic separators TECHNICON INSTRUMENTS CORP 12 April 1972 [13 April 1971] 16849/72 Heading B2J [Also in Division A5] Apparatus for the separation of lymphocytes from whole blood samples comprises a frame 46 having clamps 48 and guides 50 for supporting a hypodermic syringe 10 containing a blood sample in which leucocytes have been tagged with magnetic particles; a container 90 for the separated lymphocytes: a separator constituted by a magnet (58) with pole pieces (64), (66) Figs. 7 and 8 (not shown); and a transfer tube 74 which passes from the frame 46, through the separator and back to the frame 46, and which has sealed ends 76, 78 held in supports 92, 82 adjacent the upper ends of the container 90 and the syringe 10 respectively. In use, a drive mechanism 52 working in a slot 54 urges the plunger 14 upwardly so that the needle of the syringe 10 punctures the tube 74, the other end of which has also been punctured by a hypodermic needle 96 communicating with the container ...

IMPROVEMENTS RELATING TO CENTRIFUGES

CENTIFUGE CONTAINER FOR PLASMA SEPARATION

Liquid separating agent and liquid separating method

A liquid separating agent consisting essentially of (A) a separating layer-forming material which is a polymeric oily substance selected from the group consisting of a copolymer of an alpha -olefin with an alpha , beta -unsaturated dicarboxylic acid diester and a copolymer of a styrene with an alpha , beta unsaturated dicarboxylic acid diester and (B) an organic gelling agent which is a condensation product of a benzaldehyde with a tetra- or higher hydric alcohol.

Apparatus and method for separating certain components from blood

... 969,741. Blood test separating devices. J. H. BREWER. June 6, 1963, No. 22620/63. Heading B1X. A device for separating solid components from small blood samples comprises a member having in one surface thereof a recess comprising intercommunicating principal and auxiliary portions, the former of which contains a precipitating agent. Fig. 6 shows a sheet 10 in the form of a card or slide having a principal recess portion 12 communicating with the auxiliary recess portion to the right thereof. The sheet is packed in an aluminium foil envelope lined with polyethylene, polypropylene or polyvinyl chloride, together with a disposable lancet 22, similarly packed, a stirring rod 23 and a wire loop 24 (or a capillary pipette) for removing liquid from the recess. In use, two or three drops of blood are placed in the principal recess portion 12, stirred with the reagent to effect precipitation, and the sheet is then folded at 16 with end 17 downwardly pointing, and placed on a horizontal surface to ...

Improvements relating to bags for containing material during centrifuging

... 976,207. Flexible containers; bags. G. N. HEIN. April 8, 1963 [May 7, 1962], No. 17319/64. Divided out of 976,206. Headings B8D and B8K. A container or bag, formed as shown in Fig. IV, has a chamber 13 which may be divided from an annular chamber 11 by flexing the lower wall of the container upwards. The container, designed for use on a centrifuge, is made of transparent plastics sheets bonded together along their edges 4. The lower wall is more flexible than the upper wall.

Serum separation apparatus and method

An open-topped tube of test-tube form contains at the bottom a small quantity of solids (e.g. blood solids) and above it a liquid (e.g. blood serum). A further open-topped tube, closed at the bottom by a resilient piston head, is introduced into, and moved downwardly within, the first tube, the piston head making sealing contact with the walls of the first tube. First air above the liquid, and then the liquid, are removed through a flexible capillary tube, whose lower end protrudes downwards a short distance through a hole in the piston head, and whose upper end discharges into either a separate vessel, or the sliding tube. The apparatus may be used for separating immiscible liquids.

CENTRIFUGE

BLOOD SEPARATION MEDIA

Device

Blood sample analysis

Method and apparatus for the separation of protein bound and ionic portions of a substance

Protein bound and ionic portions of an element contained in a sample are separated by depositing the sample on a support, drying the sample and coagulating the protein of the sample, subjecting the sample to electrophoresis to remove the ionic portion of the element, exposing the remainder of the sample to an oxidizing atmosphere in a vessel that also contains a liquid, heating the remainder of the sample for destruction of the protein so that the previously protein-bound element is released and absorbing said element in said liquid and then analysing the resulting liquid to determine the amount of protein-bound element. In each of the above steps the sample is treated on the support. Protein-bound iodine in blood serum, or phosphorus, iodine or sulphur in other samples can be analysed by this method. According to a preferred embodiment a plurality of supports in the form of closely wound platinum coils are mounted in a head which may be tilted to immerse the coils in a body of deionized ...

METHOD OF SELECTIVELY REMOVING LIPOPROTEIN FROM BLOOD PLASMA OR SERUM

Cell assay kit and method

Cell assay kit and method

Cell assay kit and method

Cell assay kit and method

MEHRPROBEN-ROTORANORDNUNG ZUR HERSTELLUNG VON BLUTFRAKTIONEN

DEVICE FOR THE DETERMINATION OF THE VOLUME OF A MATERIAL LAYER

DISK SEPARATOR FOR BLOOD COMPONENTS

PROCEDURE FOR THE SEPARATION FROM RARE CELLS FROM FLUID SAMPLES

BIO SENSORS WITH POROUS CHROMATOGRAPHI DIAPHRAGMS

PROCEDURE FOR ANALYSING HEMOGLOBIN BY MEANS OF CAPILLARY ELECTROPHORESIS, KIT FOR CAPILLARY ELECTROPHORESIS AND USE OF FLOW OF RETARDER IN LOOK FOR A PROCEDURE

PROCEDURE AND DEVICE FOR THE SEPARATION FROM BLOOD COMPONENTS

VERFAHREN ZUR BESTIMMUNG DES VOLUMENS EINER MATERIALSCHICHT

BLOOD TREATMENT DEVICE WITH A SEALED DIFFUSER IN A DEVICE FOR OPTICAL CONTROL

MECHANISM TO CONTROLLED SELKTIVEN OFF DIVORCE OF UNWANTED COMPONENTS FROM BLOOD FOR THE ACHIEVEMENT OF A THERAPEUTIC EFFECT

PLASMAFILTRATION

The invention relates to a process for preparing blood plasma for determining one or more blood parameters, in particular coagulation parameters. In order to improve the measurement, the blood plasma is sucked through a filter by means of reduced pressure.

PLASMA FILTRATION

COMPOSITIONS FOR SEPARATING FROM ERYTHROZITEN FROM SERUM OR PLASMA IN BLOOD ANALYSIS SAMPLES AND PROCEDURE FUER THEIR USE.

PROCEDURE FOR THE DEMARCATION AND SEPARATION FROM CELLS IN CENTRIFUGED BLOOD TESTS.

DEVICE FOR EXTRACTING A CERTAIN LIQUID PHASE.

PROCEDURE AND EQUIPMENT FOR THE SEPARATION OF A BODY FLUID FROM PARTICLES IN THIS LIQUID AND TEST RECORD FOR THIS SEPARATION AND ANALYSIS OF THE BODY FLUID.

PROCEDURE FOR THE SEPARATION FROM HIGH DENSITY LIPOPROTEIN FROM BLOOD TESTS

ROTOR AND PROCEDURE FOR THE AUTOMATIC PROCESSING OF LIQUIDS FOR LABORATORY AND BIOANALYTIC PURPOSES

COMPOSITION FOR THE SEPARATION FROM BLOOD.

DEVICE AND PROCEDURE FOR THE SEPARATION FROM BLOOD PHASES.

METHOD FOR THE MEASUREMENT OF THE HAEMOGLOBINGEHALTS.

DEVICE FOR THE ADMISSION, TO TRANSPORT AND TO THE PRODUCTION OF CELL MATERIAL AS WELL AS TO THE TRANSMISSION OF THE WON CELL MATERIAL ON SLIDES.

DIAGNOSTIC KITS AND METHODS TO THE PROOF OF GRANULOZYTEN

DEVICE AND PROCEDURE FOR THE BLOOD SEPARATION AND PREPARATION PROCEDURE, QUANTIFICATION PROCEDURE AND PRESERVATION CONTAINER FOR A BIOLOGICAL SAMPLE

METHOD TO ERREICHERUNG OF POPULATIONS OF RARE CELLS

PROCEDURE AND EQUIPMENT FOR THE BLOOD TEST ANALYSIS

PROCEDURE FOR THE DETERMINATION OF THE DOSE OF HÄMATOPOETI MAIN CELL TRANSPLANT ION UNITS, TO INCREASING CELLS NEEDS

DEVICE AND PROCEDURE FOR MOVING FLUIDS OF MEANS CENTRIFUGAL ACCELERATION DURING THE AUTOMATIC LABORATORY TREATMENT

GRAPHITIC FLEECES AS FUNCTIONAL ONE LAYERS IN DIAGNOSTIC TEST KITS

A system and method for calibrating a centrifuge

A sample holder for use in a centrifuge, the sample holder being generally planar and comprising: an aperture or recess for releasably retaining a sample storage member including a sample chamber adapted to contain a volume of liquid; a centre point around which the holder will rotate during use; and one or more calibration features, wherein the calibration feature(s) comprise one or more outer edges, which lie on the side of the or each calibration feature which is furthest from the centre point, and the one or more outer edges comprise a series of radially spaced-apart outer edge portions or positions which are spaced at different distances from the centre point as a function of angular position around the centre point.

Blood plasma separation device

A device for separating blood plasma from whole blood includes a first reservoir and a second reservoir. The first reservoir is configured to receive a sample of whole blood including red blood cells and includes a collection region and a constricted region. The second reservoir is fluidically connected to the constricted region of the first reservoir, such that, responsive to centrifugal force applied to the device, the sample of whole blood disposed within the first reservoir separates into a first fraction and a second fraction. The first fraction is located in the collection region and includes blood plasma from which substantially all red blood cells have been removed. The second fraction is located in the second reservoir and includes blood plasma and red blood cells that have been removed from the first fraction by the centrifugal force. The constricted region inhibits the second fraction from entering the collection region.

BLOOD SEPARATION SYSTEM

APPARATUS FOR SEPARATING PHASES OF BLOOD

FILTER WITH SEPARATE FEED AND ELUANT ENTRY PORTS

Method and apparatus for making density gradients

Polyesters for use in blood partitioning

BUFFY COAT SEPARATOR FLOAT SYSTEM AND METHOD

METHOD AND APPARATUS FOR SEPARATION OF PARTICLES IN A MICROFLUIDIC DEVICE

METHOD AND APPARATUS OF HEMORHEOMETER

Centrifugal separation kit and methods for centrifugal separation using the same

A centrifugal separation kit is used for centrifugally separating whole blood and body fluid, and a method for centrifugal separation using the kit. The centrifugal separation kit is capable of easily extracting a target substance by installing an injector to a centrifugal separation tube and collecting the centrifugally separated target substance with the injector.

Centrifuge and separation vessel therefore

The centrifugation vessel includes an outer wall containing an interior space. A dam defines a barrier which divides the interior space into at least two regions including a catch basin defining a higher gee region and a reservoir defining a lower gee region. These regions are joined together over the dam. The dam includes a face which is preferably tapered to enable optimization of speed of separation of a sample placed within the vessel. The vessel is usable in a biological sample processing method by having the higher gee region of the vessel configured to have an elongate form and the volume optimized for collection of a higher density fraction of the sample. Supply and withdrawal tubes extend into the regions for reliable extraction and separate collection of differing density fractions after separation by centrifugation.

Devices and Process for Separating Plasma From a Blood Sample

The present invention pertains to a device for separating plasma from a blood sample comprising a stacked structure which is provided with a first portion including a separating member having a first surface for applying or receiving the blood sample, wherein the separating member is adapted to permit the passage of plasma but to inhibit the passage of cells, and a second portion including an absorptive member for absorbing the plasma, which has a second surface in contact with the separating member for receiving the plasma, wherein the absorptive member is adapted to generate a capillary pressure so as to draw plasma from the separating member to the absorptive member. The first portion is fixed to the backing member in a manner to be removed without destroying the absorptive member. The absorptive member is fixed to the backing member in a manner to be removed without destroying the absorptive member.

Disease diagnosing biosensor capable of promptly separating blood cells

The present invention relates to a disease diagnosing biosensor capable of promptly separating blood cells, in which a disease can be analyzed further accurately by adding a blood cell separating pad on a front or rear side of a capillary tube and separating the blood cells and plasma within 1 or 2 seconds owing to distribution of hydrophilic and hydrophobic segments. The entire configuration of the biosensor according to the present invention includes a base film; electrodes arranged on a top surface of the base film; a space film covering a top surface of the electrodes; a blood cell separating pad connected to a rear side of the space film; a capillary tube added on a rear top surface of the space film; and a cover film covered on a top surface of the capillary tube and the blood cell separating pad.

Multiple laminar flow-based particle and cellular separation with laser steering

The invention provides a method, apparatus and system for separating cellular components, and can be combined with holographic optical trapping manipulation or other forms of optical tweezing. One exemplary method includes providing a first flow having a plurality of components; providing a second flow; contacting the first flow with the second flow to provide a first separation region; and differentially sedimenting a first cellular component of the plurality of components into the second flow while concurrently maintaining a second cellular component of the plurality of components in the first flow. The second flow having the first cellular component is then differentially removed from the first flow having the second cellular component. Holographic optical traps may also be utilized in conjunction with the various flows to move selected components from one flow to another, as part of or in addition to a separation stage.

Filter device

A filter device of the present disclosure includes a first port from which a solution containing a substance is to be input, and a first flow passage communicating with the first port. A filter portion made of a plurality of fibrous substances including inorganic oxide is formed in at least one part in the first flow passage. The plurality of fibrous substances has one peak in the diameter distribution.

Apparatus and method for processing biological material

The application discloses an apparatus and method for processing biological material, including a suspension of cells.

Arrangement for detection of hemolysis

The present invention relates to a device for visual detection of hemolysis in a whole blood sample, comprising at least one visible detection compartment and a transfer passage connected to said visible detection compartment, said transfer passage being arranged to permit transfer of a volume of plasma from said sample to said detection compartment and wherein said transfer passage further is arranged with a separation device ( 4 ) for separating plasma from blood cells within said whole blood sample before said plasma reaches the detection compartment, wherein said device is arranged with subpressure means providing a subpressure inside said detection compartment for generating a force urging said volume of plasma to be transferred from said whole blood sample to said detection compartment through said transfer passage and via said separation device.

System and method for blood separation by microfluidic acoustic focusing

Systems and methods for cleansing blood are disclosed herein. The methods include acoustically separating undesirable particles bound to capture particles from formed elements of whole blood. After introducing the capture particles to whole blood containing undesirable particles, the whole blood and capture particles are flowed through a microfluidic separation channel. At least one bulk acoustic transducer is attached to the microfluidic separation channel. A standing acoustic wave, imparted on the channel and its contents by the bulk acoustic transducer, drives the formed elements and undesirable particles bound to capture particles to specific aggregation axes. After aggregating the particles, the formed elements exit the separation channel through a first outlet and are returned to the patient. The undesirable particles, bound to the capture particles, exit through a second outlet and can be discarded to saved for later study.

Microfluidic device

A micro-fluidic device includes a filter cabinet, a first filtering unit and a second filtering unit. The filter cabinet includes a first path and a second path branched from the first path. The first path and the second path are formed within the filter cabinet so that a sample containing different kinds of targets can flow through the first path and the second path. The first filtering unit is installed in an upstream portion of the first path to filter the different kinds of targets from the sample, the first filtering unit configured to guide the different kinds of targets toward the second path. The second filtering unit is installed in the second path to receive the different kinds of targets from the first filtering unit and to filter the different kinds of targets on a size-by-size basis.

Sample cell for fluid analysis system

Systems and method are disclosed for determining a concentration of an analyte (e.g., glucose) in a fluid (e.g., blood). The system can draw blood from a patient and deliver the blood to a sample cell. A centrifuge motor can spin the sample cell to separate the fluid into a plurality of components (plasma, red blood cells, etc.). A particular component of the fluid (e.g., plasma) may be positioned at a sample portion of the sample cell after centrifuging such that the concentration of the analyte is measured in the particular component of the fluid (e.g., plasma). The sample cell can include a cuvette that has two window pieces sandwiched between two clamshell pieces, and where the sample portion of the sample cell is defined by a gap between the window pieces.

Apparatus and method for separating and concentrating fluids containing multiple components

An apparatus that allows for separating and collecting a fraction of a sample. The apparatus, when used with a centrifuge, allows for the creation of at least three fractions in the apparatus. It also provides for a new method of extracting the buffy coat phase from a whole blood sample. A buoy system that may include a first buoy portion and a second buoy member operably interconnected may be used to form at least three fractions from a sample during a substantially single centrifugation process. Therefore, the separation of various fractions may be substantially quick and efficient.

Membrane suitable for blood filtration

The invention relates to a membrane construction comprising multiple layers wherein at least one of the layers is a nanoweb made of polymeric nanofibers, wherein the mean flow pore size of the nanoweb is in the range from 50 nm to 5 μm, wherein the number average diameter of the nanofibers is in the range from 100 to 600 nm, wherein the basis weight of the nanoweb is in the range from 1 to 20 g/m 2 , wherein the porosity of the nanoweb is in the range from 60 to 95%, wherein at least one of the layers is a support layer and wherein the nanoweb is hydrophilic.

CENTRIFUGE

A centrifuge is provided. The centrifuge includes a power source, the power source configured to generate electrical power from a renewable power source. At least one battery electrically coupled to the power source. A motor is electrically coupled to the at least one battery. A rotor is coupled to the motor. The rotor has a generally cylindrical body and a pair of opposing openings opposite the motor, and a pair of holders each disposed in one of the pair of opposing openings, each of the holders having an opening on one end sized to receive a capillary tube. 1. A centrifuge comprising:a power source configured to generate electrical power from a renewable power source;at least one battery electrically coupled to the power source;a motor electrically coupled to the at least one battery; anda rotor coupled to the motor, the rotor having a generally cylindrical body and a pair of opposing openings opposite the motor, and a pair of holders each disposed in one of the pair of opposing openings, each of the pair of holders having an opening on one end sized to receive a capillary tube.2. The centrifuge of wherein the power source is a solar panel.3. The centrifuge of wherein the solar panel includes five 1000 mA claim 2 , 0.55W solar panels.4. The centrifuge of wherein the pair of opposing openings are disposed on an angle.5. The centrifuge of wherein the angle is 65 degrees from a horizontal plane.6. The centrifuge of wherein the at least one battery includes a first battery and a second battery electrically coupled in parallel between the power source and the motor.7. The centrifuge of further comprising a controller configured to selectively couple the first battery to the motor.8. The centrifuge of wherein the controller is configured to charge the second battery with the power source when the first battery is coupled to the motor.9. A method of evaluating a patient's hematocrit comprising:separating a sample of blood in a capillary tube into a red blood cell ...

Device for performing a diagnostic test and methods for use thereof

Assay cassettes and testing devices that can be used to provide rapid, accurate, affordable, laboratory-quality testing at the point of care. Such assay cassettes and testing devices are designed to provide rapid, quantitative test results in a point-of-care setting or the like. Likewise, such assay cassettes and testing devices may eliminate or replace expensive, centralized clinical testing equipment and technical personnel. Such testing device may include automated data reporting and decision support. Methods for performing point of care diagnostic tests are also disclosed.

Systems and methods for separation and analysis of target analytes

Systems for analyzing multiple target analytes of a suspension include a tube and multiple floats. The target analytes are conjugated with at least one fluorescent marker, and the tube, multiple floats and suspension are centrifuged so that at least a portion of each target analyte is located between the inner wall of the tube and the surface of the respective float. In order to identify each target analyte, the portions of the suspension between the tube and each float is illuminated with one or more channels of excitation light, which causes the at least one fluorescent marker to become excited and emit light at longer wavelengths. One of the target analytes may be used to assess quality control or may aid in a subsequent analysis or diagnosis.

DENSITY-BASED SEPARATION OF BIOLOGICAL ANALYTES USING MULTIPHASE SYSTEMS

The disclosed methods use a multi-phase system to separate samples according to the density of an analyte of interest. The method uses a multi-phase system that comprises two or more phase-separated solutions and a phase component such as a surfactant or polymer. The density of the analyte of interest differs from the densities of the rest of the sample. The density of the analyte of interest is substantially the same as one or more phases. Thus, when the sample is introduced to the multi-phase system, the analyte of interest migrates to the phase having the same density as the analyte of interest, passing through one or more phases sequentially. 1. A method of analyzing or separating a sample comprising one or more biological analytes of interest using a multi-phase system , comprising: each phase comprises a phase component selected from the group consisting of a polymer, a surfactant and combinations thereof, wherein at least one phase comprises a polymer;', 'each said phase has an upper and a lower phase boundary; and, 'a) providing a multi-phase system comprising two or more phase-separated solutions, wherein'}each of the two or more phases has a different density and the phases, taken together, represent a density gradient; andb) introducing a biological sample comprising one or more biological analytes of interest without disrupting the phase-separated solution; andc) allowing each of the biological analytes to migrate to a location in the multi-phase system that is characteristic of its density, wherein during migration the sample contacts one or more of the two or more phases sequentially.2. The method of claim 1 , wherein the at least two phases share a common solvent.3. The method of wherein the multi-phase system is an aqueous system and the common solvent is an aqueous solvent.4. The method of claim 1 , wherein the multi-phase system is a non-aqueous system and the common solvent is an organic solvent.5. The method of claim 1 , wherein the phase ...

Apparatus and method for the automated detection of phases for automated analysis

An apparatus and a method are provided for the detection of the level of the different phases present in at least one tube or vessel intended for filling the different wells of a microplate-format container for an automated analysis system. The apparatus is equipped with a fixing portion arranged to allow it to be itself held and/or handled instead of a microplate-format container, and carries out this detection by measuring the variation in wavelength of light reflected on a point zone of the content of the tube, during a displacement of the tube along an optical reader or of the optical reader along the tube in a rectilinear movement in a known manner. Such a device or method is also operated by illumination with monochromatic light and detection of the amount of reflected light, with the phase differences being recognized by the sudden variation in the amount of reflected light.

Method and apparatus for sensing ion concentrations in a fluid sample

The invention provides a method for the measurement of a concentration of a charged species in a sample, the sample having a plurality of types of charged species and at least one insoluble component. The method comprises: providing the sample on a surface of a partly permeable layer; allowing components of the sample to pass through the partly permeable layer into a channel; and separating the components into sections, such that each at least one of the sections substantially comprises a single type of the plurality of the types of charged species, and determining the charge concentration in the at least one of the sections.

APPARATUS AND METHOD FOR ISOLATING LEUKOCYTES AND TUMOR CELLS BY FILTRATION

The present invention provides novel apparatuses and methods for isolating or recovering a subset of blood cells such as leukocytes and/or circulating tumor cells from blood samples by filtration without changing the intracellular concentration of a therapeutic agent such as an anticancer drug. Contrary to the art, the apparatuses and methods of the present invention advantageously provide cell lysates from recovered cells such as leukocytes and/or circulating tumor cells without substantial dilution of a therapeutic agent such as an anticancer drug. 1. An apparatus for isolating and separating leukocytes from red blood cells in a whole blood sample , said apparatus comprising:a filtration device comprising an upper chamber, a lower chamber, and one or more stacked filter membranes between said upper and lower chambers, wherein said one or more stacked filter membranes are capable of retaining said leukocytes; anda collection tube for collecting red blood cells from said whole blood sample, wherein said filtration device is placed on top of said collection tube, and wherein said red blood cells are separated from said leukocytes and collected in said collection tube following centrifugation.2. The apparatus of claim 1 , wherein said whole blood sample is loaded into said upper chamber of said filtration device.3. The apparatus of claim 1 , wherein said filtration device comprises two claim 1 , three claim 1 , or four stacked filter membranes.4. The apparatus of claim 1 , wherein said upper chamber further comprises a snap-cap lid attached thereto.5. The apparatus of claim 1 , further comprising a second collection tube claim 1 , wherein said collection tube containing said red blood cells is replaced with said second collection tube following centrifugation.6. The apparatus of claim 5 , wherein a lysate of said leukocytes is collected in said second collection tube following the addition of lysis buffer to said upper chamber and centrifugation.7. The apparatus of ...

Device for filtering blood

A device is proposed having a separating device for filtering a liquid sample and having a conveying device. A positive pressure or negative pressure can be generated by means of the conveying device, for accelerating or assisting the filtration.

Ex-Vivo Multi-Dimensional System For The Separation And Isolation Of Cells, Vesicles, Nanoparticles, And Biomarkers

Devices and techniques are described that involve a combination of multidimensional electrokinetic, dielectrophoretic, electrophoretic and fluidic forces and effects for separating cells, nanovesicles, nanoparticulates and biomarkers (DNA, RNA, antibodies, proteins) in high conductance (ionic) strength biological samples and buffers. In disclosed embodiments, a combination of continuous and/or pulsed dielectrophoretic (DEP) forces, continuous and/or pulsed field DC electrophoretic forces, microelectrophoresis and controlled fluidics are utilized with arrays of electrodes. In particular, the use of chambered DEP devices and of a properly scaled relatively larger electrode array devices that combines fluid, electrophoretic and DEP forces enables both larger and/or clinically relevant volumes of blood, serum, plasma or other samples to be more directly, rapidly and efficiently analyzed. The invention enables the creation of “seamless” sample-to-answer diagnostic systems and devices. The devices and techniques described can also carry out the assisted self-assembly of molecules, polymers, nanocomponents and mesoscale entities into three dimensional higher order structures.

METHOD AND INSERT FOR DENSITY GRADIENT SEPARATION

An insert for a centrifuge tube suitable for use in density gradient separation is described. The insert includes a member sized to fit within the tube for dividing the tube into a top portion and a bottom portion. Optionally the insert has a support extending or depending from the member for positioning the member within the tube. At least two openings are located on the member so that a first opening is closer to a bottom end of the tube relative to a second opening when the insert is positioned in the centrifuge tube. Also described are methods for separating a target population of cells from a sample using the insert for a centrifuge tube. 1. An insert for a centrifuge tube , the insert comprising:a member sized to fit within the tube for dividing the tube into a top portion and a bottom portion, andat least two openings through the member so that a first opening is closer to a bottom end of the tube relative to a second opening when the insert is positioned in the centrifuge tube.2. The insert of claim 1 , wherein the first opening allows fluid communication between a space in the top portion of the tube above the member and a space in the bottom portion of the tube below the member when the insert is positioned in the centrifuge tube and wherein the second opening allows air to escape from the space below the member when the bottom end of the tube is filled with a liquid through the first opening.3. (canceled)4. The insert of claim 1 , wherein the at least two openings are sized to provide surface tension across the openings to prevent a liquid contained below the insert to flow through the openings when the tube is inverted.5. The insert of claim 1 , wherein the first opening is located at a lowest part of a top surface of the member when the insert is positioned in the centrifuge tube.6. The insert of claim 1 , wherein the second opening is located at a highest part of a top surface of the member when the insert is positioned in the centrifuge tube.7. The ...

Isolating Target Cells From A Biological Fluid

A micro-fluidic device operable to isolate target cells from a biological fluid comprises: an inlet operable to receive the biological fluid, the biological fluid comprising target cells and other components; a waste outlet operable to receive at least the other components of the biological fluid; a plurality of parallel arrays of cell isolation wells coupling the inlet with the waste outlet, each parallel array of cell isolation wells supporting a flow of the biological fluid from the inlet to the waste outlet in response to a pressure differential thereacross, each array of cell isolation wells comprising a plurality isolation wells, each isolation well being dimensioned to mechanically trap the target cells therein whilst permitting flow of other components of the biological fluid; and at least one pressure maintenance structure operable to assist in maintaining a predetermined pressure differential across each of the plurality of parallel arrays of cell isolation wells. 1. A micro-fluidic device operable to isolate target cells from a biological fluid , said micro-fluidic device comprising:an inlet operable to receive said biological fluid, said biological fluid comprising target cells and other components;an waste outlet operable to receive at least said other components of said biological fluid;a plurality of parallel arrays of cell isolation traps coupling said inlet with said waste outlet, each parallel array of cell isolation traps supporting a flow of said biological fluid from said inlet to said waste outlet in response to a pressure differential thereacross, each cell isolation trap being dimensioned to mechanically trap said target cells therein whilst permitting flow of other components of said biological fluid;at least one pressure maintenance structure operable to assist in maintaining a predetermined pressure differential across each of said plurality of parallel arrays of cell isolation traps,a buffer port for reversing the flow through the ...

Procoagulant factors suitable for subsequent autologous use

Provided is a blood collection unit suitable for accelerated blood coagulation of whole blood for subsequent autologous or allogeneic use. The blood collection unit includes an inside surface that an activation site accelerating coagulation by having a high roughness. Further provided is a blood collection unit including an inside surface that has an activation site having a high roughness area, and an interior of the blood collection unit has been prepared with a pressure of no more than 255 mBar, and preferably a pressure of no more than 130 mBar.

DEVICES FOR SEPARATION OF PARTICULATES, ASSOCIATED METHODS AND SYSTEMS

A separation device, system and associated method are provided herein for separation of particulates form a base fluid. The separation device comprises a first microchannel comprising a fluid inlet and a mesofluidic collection chamber. The mesofluidic collection chamber has a first side and a second side, wherein the mesofluidic collection chamber is operatively coupled to the first microchannel on the first side, and wherein the mesofluidic collection chamber comprises a first fluid outlet at the second side, such that the fluid inlet, first microchannel, and first fluid outlet are in fluidic communication via the mesofluidic collection chamber. 1. A separation device for separating particulates dispersed in a base fluid , the device comprising:a fluid inlet;a fluid outlet;a first microchannel disposed between the fluid inlet and the fluid outlet;a microporous body defining at least a portion of the first microchannel; anda mesofluidic collection chamber on one side of the microporous body;wherein the particulates dispersed in the base fluid traverse through the first microchannel under an influence of a force field, andwherein the particulates delaminate from the base fluid in the first microchannel and trace a fluidic expansion while entering to the mesofluidic collection chamber, and wherein at least a portion of the particulates in at least a portion of the base fluid are collected in the mesofluidic collection chamber.2. The device of claim 1 , further comprising a second microchannel having a second fluid outlet disposed at the second side of the mesofluidic collection chamber claim 1 , such that the fluid inlet claim 1 , first microchannel claim 1 , second microchannel claim 1 , and second fluid outlet are in a fluidic communication via the mesofluidic collection chamber claim 1 , and wherein the second microchannel has a length lin a range from about 5 millimeters to about 100 millimeters and a height h.3. The device of claim 2 , further comprising an input ...

MAGNETIC SEPARATION DEVICE AND METHOD OF USE

The current invention relates to the method and apparatus to magnetically separate biological entities with magnetic labels from a fluid sample. The claimed magnetic separation device removes biological entities with magnetic labels from its fluidic solution by using a soft-magnetic center pole with two soft-magnetic side poles. The claimed device further includes processes to dissociate entities conglomerate after magnetic separation. 1. A device for separating biological entities comprising:a magnetic field source having a first surface and a second surface;a soft magnetic center pole having a first end contacting said first surface, and a tip end tapering away from said first end, thereby concentrating magnetic flux from said first surface towards said tip end;a first soft magnetic side pole having a first base end and a second end;a second soft magnetic side pole having a second base end and a third end;a channel contacting said tip end contains a fluidic sample comprising said biological entities, thereby causing separation of said biological entities bound with magnetic labels towards said tip end;wherein said first and second base ends contact said second surface;wherein said second and third ends are located on opposing sides of said tip end;wherein said magnetic flux from said tip end divides into each of said second and third ends, thereby forming a flux closure within said center pole and said first and second side poles; andwherein said biological entities bound with magnetic labels are removed from said channel after separating said channel from said tip end.2. The device according to claim 1 , wherein said magnetic field source comprises a permanent magnet having a first polarization surface contacting a top surface of a soft magnetic shield claim 1 , and a second polarization surface opposing said first polarization surface claim 1 , wherein said first surface is said second polarization surface and said second surface is said top surface.3. The ...

Device and Method for Separating A Fluid Mixture such as Blood

A lab-on-chip device for the processing, in particular the separation, of a fluid mixture comprising two immiscible phases (liquid and/or solid), said device comprising a fluid line () which successively includes an inlet reservoir (), a separation channel (), a collection channel () and an outlet (), said separation channel () being designed in a way as to allow a separation of the fluid mixture into said two phases. 118- (canceled)19. A lab-on-chip device for processing a fluid mixture having two immiscible phases , the device comprising:a fluid line which successively includes an inlet reservoir, a separation channel, a collection channel, and an outlet,wherein the separation channel is configured to allow a separation of the fluid mixture into the two immiscible phases.20. The device according to claim 19 , wherein a size of the separation channel is configured to induce sedimentation and separation of the fluid mixture and generation of a purified fluid by capillary action.21. The device according to claim 19 , further comprising:an air bubble actuator configured to, when actuated, generate an air bubble into the fluid line to isolate a defined volume of processed fluid.22. The device according to claim 19 , the device being configured to generate a purified fluid by capillary driven forces within the separation channel.23. The device according to claim 19 , the device configured to generate a purified fluid by sedimentation within the separation channel.24. The device according to claim 19 , wherein dimensions of the separation channel and wettability of surfaces of the separation channel are configured to allow precise control of a filling speed.25. The device according to claim 19 , wherein dimensions of the separation channel and wettability of surfaces of the separation channel are configured to allow precise control of a shape of a front flow of the fluid mixture.26. The device according to claim 19 , further comprising:a metering channel that ...

SHEATH FLUID SYSTEMS AND METHODS FOR PARTICLE ANALYSIS IN BLOOD SAMPLES

Aspects and embodiments of the instant disclosure provide a particle and/or intracellular organelle alignment agent for a particle analyzer used to analyze particles contained in a sample. An exemplary particle and/or intracellular organelle alignment agent includes an aqueous solution, a viscosity modifier, and/or a buffer. 1. A particle and intracellular organelle alignment liquid (PIOAL) for use in a combined viscosity and geometric hydrofocusing analyzer , the PIOAL directing flow of a blood sample fluid of a given viscosity that is injected into a narrowing flowcell transition zone of the visual analyzer so as to produce a sample fluid stream enveloped by the PIOAL , the PIOAL comprising:a fluid having a higher viscosity than the viscosity of the blood sample fluid,a pH adjusting agent, andProcaine HCl,wherein a viscosity hydrofocusing effect induced by an interaction between the PIOAL fluid and the sample fluid associated with the viscosity difference, in combination with a geometric hydrofocusing effect induced by an interaction between the PIOAL fluid and the sample fluid associated with the narrowing flowcell transition zone, is effective to provide a target imaging state in at least some of the plurality of particles at an imaging site of the visual analyzer while a viscosity agent in the PIOAL retains viability of cells in the sample fluid stream leaving structure and content of the cells intact when the cells extend from the sample fluid stream into the flowing sheath fluid, andwherein the viscosity agent of the sheath fluid comprises glycerol at a concentration between about 1 to about 50% (v/v).2. The PIOAL of claim 1 , wherein the viscosity agent of the sheath fluid comprises polyvinylpyrrolidone (PVP).3. The PIOAL of claim 2 , wherein the polyvinylpyrrolidone (PVP) is at a concentration of 1% (w/v).4. The PIOAL of claim 1 , wherein the viscosity agent of the sheath fluid comprises glycerol at a concentration of 5% (v/v) and polyvinylpyrrolidone (PVP) ...

METHODS FOR EXTRACTING PROTEINS FROM BLOOD PLASMA

A method of producing protein products including alpha-1-proteinase inhibitor, gamma globulin, albumin, and other proteins from plasma includes steps of: (1) adding a salt to the blood product to produce a first intermediate, wherein the salt comprises between 11-13 wt % of the first intermediate; (2) separating the first intermediate to produce a first supernatant and a first paste; (3) adding a salt to the first intermediate to produce a second intermediate, wherein the salt comprises between 21-23 wt % of the second intermediate; (4) separating the second intermediate to produce a second supernatant and a second paste; (5) separating a third intermediate from the second supernatant by affinity chromatography; and (6) separating the third intermediate by ion exchange chromatography to produce an eluate containing the protein product. Advantageously, the inventive methods are simple and produce alpha-1-proteinase inhibitor, gamma globulin, albumin, and other proteins in high yields. 1. A method of separating A1P1 from a blood plasma containing product , the method comprising:thawing a frozen blood plasma product followed by stirring at a temperature suitable for dissolving a cryoprecipitate to generate the blood plasma containing product;adding a salt to the blood plasma containing product to produce a first intermediate, wherein the salt comprises between 11-20 wt % of the first intermediate;separating the first intermediate to produce a first supernatant and a first paste;adding a salt to the first supernatant to produce a second intermediate, wherein the salt comprises between 15-30 wt % of the second intermediate;separating the second intermediate to produce a second supernatant and a second paste;separating the second supernatant by affinity chromatography using an A1P1-specific affinity media to generate a flow-through fraction and a first eluate, wherein the first eluate comprises A1P1.2. The method of claim 1 , wherein the step of adding the salt to the ...

SOLUTION COLLECTION DEVICE WITH EVALUATION ELEMENT

A blood testing device for detecting hemolysis in a blood sample is described. The blood testing device comprises an housing for containing the blood sample. The housing has a treatment window and an optical zone formed therein. The blood testing device further includes an acoustic transducer positioned to selectively generate acoustic forces directed into the treatment window of the housing and a control unit for selectively actuating and deactuating the acoustic transducer to permit colorimetric analysis of plasma within the blood sample. 1. A blood testing assembly , comprising: a housing constructed of a fluid impermeable material;', 'a treatment window formed in the housing, the treatment window constructed of a material capable of passing acoustic forces into the housing;', 'an optical zone formed in the treatment window of the housing, the optical zone constructed of a material that allows a colorimetric analysis of a blood sample positioned within the housing, and located adjacent to the optical zone; and, 'a blood testing device, comprising an acoustic transducer position to selectively generate acoustic forces directed through the treatment window of the housing and into the blood sample; and', 'a control unit for selectively actuating and deactuating the acoustic transducer., 'a reader, comprising2. A method , comprising:separating blood cells from plasma within a blood sample into a first zone containing plasma and blood cells, and a second zone containing plasma and being substantially devoid of blood cells; andcolorimetrically analyzing the plasma within the second zone to determine a degree of hemolysis within the blood sample. The subject application claims benefit under 35 USC § 119(e) of U.S. provisional Application No. 62/776,825, filed Dec. 7, 2018. The entire contents of the above-referenced patent application are hereby expressly incorporated herein by reference.Not Applicable.Point-of-care testing refers generally to medical testing at or near ...

AN APPARATUS FOR THE SAMPLING AND PREPARATION OF BIOMEDICAL SAMPLES IN A CLOSED STERILE ENVIRONMENT

An apparatus for sampling, preparing and collecting biomedical samples for a subsequent diagnostic, clinical and/or therapeutic use in a closed sterile environment is provided. 1. An apparatus for sampling , processing and collecting biomedical samples for a subsequent diagnostic , clinical and/or therapeutic use , in a closed sterile environment , said apparatus comprising: one or more sampling means of biomedical samples , one or more collecting means of said samples for diagnostic use , one or more containers for processing of said samples , each housed inside a hollow body having a diameter greater than the diameter of said containers and able to slide upwards and downwards on the outer surface of said containers , said hollow body being closed at the top by a cap centrally perforated by a hollow element facing the inside of the container and able to put it into fluid communication with the outside , said cap being able to stretch upwards or downwards in order to vary the height of said hollow element integral therewith , inside said container; and one or more collecting means for clinical or therapeutic use of said processed samples coming from said containers , said means being connected between each other by a device for the distribution of fluids consisting of rigid or flexible pipes , which let said sample flow from said sampling means to said collecting means or from said sampling means to said containers and , from these , to said collecting means , thus creating an environment which is closed and able to maintain internal sterile conditions.2. The apparatus according to claim 1 , wherein said container and/or said collecting means are individually enclosed in sterile casings.3. The apparatus according to claim 1 , wherein said container claim 1 , said hollow body claim 1 , said sterile casings and/or said collecting means are made of a transparent plastic material claim 1 , and tags are affixed thereon with bar codes or other identification means of the ...

BLOOD COMPONENTS SEPARATOR DISK

A separator disk for use in centrifugal separation of components is designed to automatically position itself during separation at the interface between the supernatant and the remaining components. Preferably the interface is between plasma and red blood cells. 1. In combination, a tube adapted to contain a fluid to be subjected to centrifugation and a separator disk in said tube adapted to separate components of said fluid having different specific gravities, said disk being made of a material having a specific gravity near the specific gravity of the components at an interface. This application is a continuation of U.S. application Ser. No. 13/680,350 filed on Nov. 19, 2012, which is a continuation of U.S. application Ser. No. 12/453,577 filed on May 15, 2009, which is a divisional of U.S. application Ser. No. 11/206,869 filed Aug. 19, 2005, now U.S. Pat. No. 7,547,272, which is a divisional of U.S. application Ser. No. 10/019,680 filed Jan. 4, 2002, now U.S. Pat. No. 7,077,273, which was the national stage of International Application No. PCT/US01/11732 filed Apr. 27, 2001, which was published in English, and claims priority of U.S. Provisional Application No. 60/200,150 filed Apr. 28, 2000.This invention relates to methods and apparatus for use in the separation of fluids into components having different specific gravities. The invention finds particular utility in the centrifugal separation of the components of blood.Centrifugal separation of blood into components of different specific gravities, such as red blood cells, white blood cells, platelets, and plasma is known from U.S. Pat. No. 5,707,331 (Wells). The apparatus shown in that patent employs a disposable processing tube having two chambers, and blood to be separated into components is placed in one of the chambers. The processing tube is placed in a centrifuge, which subjects the blood to centrifugal forces to separate the components. The supernatant is then automatically decanted into the second of ...

METHODS AND SYSTEMS FOR SEPARATING BIOLOGICAL PARTICLES

The present disclosure provides methods and systems for separating one or more target analytes from a fluid sample. The systems may comprise a microfluidic device. The microfluidic device may comprise a fluidic channel having an array of obstacles disposed therein. The array of obstacles may be oriented at an angle greater than 0° relative to a direction of a fluid flow in the fluidic channel. The array of obstacles may be configured to separate the target analytes from the fluid upon flow of the fluid through the fluidic channel. The methods of the present disclosure may comprise separating target analytes from a fluid using a microfluidic device comprising obstacles disposed in a fluidic channel of the device. The target analytes may be separated with a high efficiency, sensitivity and/or specificity. 150-. (canceled)51. A microfluidic device , comprising:a fluidic channel; andan array of obstacles disposed in said fluidic channel, and oriented at an angle greater than 0° relative to a direction of a fluid flow in said fluidic channel,wherein an obstacle of said array of obstacles is oriented at an angle of less than 90° with respect to a surface of said obstacles, and wherein said array of obstacles is configured to separate one or more target analytes from a fluid flowing through said fluidic channel.52. The microfluidic device of claim 51 , wherein said array of obstacles is oriented at an angle between about 5° and 30° relative to said direction of said fluidic flow.53. The microfluidic device of claim 51 , wherein a distance between said array of obstacles and a side wall of said fluidic channel increases along said direction of said fluid flow.54. The microfluidic device of claim 51 , wherein individual obstacles of said array of obstacles have a quadrilateral cross-section.55. The microfluidic device of claim 54 , wherein said quadrilateral cross-section is a parallelogram cross-section.56. The microfluidic device of claim 51 , wherein each obstacle of said ...

APPARATUS AND METHODS FOR PROCESSING BLOOD

Apparatus and methods for processing blood are disclosed in which one variation generally comprises a tube defining a channel and an access tube extending into the channel. An open cell matrix configured to entrap red blood cells may be positioned within at least a portion of the channel. Another variation generally comprises a cylindrical tube and a plunger slidably positioned within the channel. The plunger also has a funnel positioned upon the plunger and is movable therewith. Both the plunger and funnel define a fluid channel through and in communication with the cylindrical tube. 1. A separation apparatus , comprising:a cylindrical tube defining an opening and a channel extending therethrough;a plunger defining a plunger fluid opening and slidably positioned within the channel; anda funnel positioned upon the plunger and movable therewith, wherein the funnel defines a funnel fluid opening in fluid communication with the plunger fluid opening.2. The apparatus of further comprising a tubing removably coupled to the second fluid opening of the funnel and extending through the opening of the cylindrical tube.3. The apparatus of wherein the cylindrical tube defines a closed floor opposite of the opening of the cylindrical tube.4. The apparatus of wherein the funnel is positioned upon a first side of the plunger in proximity to the opening of the cylindrical tube.5. A method of separating components from blood claim 1 , comprising:introducing a volume of blood through a funnel and a plunger and into a channel defined by a cylindrical tube such that the funnel and plunger are moved from a first position within the tube to a second position in proximity to an opening defined by the tube;applying a centrifugal force to the volume of blood contained within the tube such that the blood forms at least a first fractional layer and a second fractional layer; andwithdrawing at least the first fractional layer from the tube via the funnel and the plunger such that the funnel ...

PARTICLE SIZE PURIFICATION METHOD AND DEVICES

A panicle separation multi-membrane matrix device and method are provided. The particles isolated may comprise noun-scale particles, such extracellular membrane vesicles, having a size of about 50 to about 150 nm. The vesicles are released by many different cell types, and may be efficiently isolated at high yield and purity according to the present methods from various body fluids (e.g., blood, saliva, breast milk, serum, plasma, ascites fluid, etc.). Such isolated exosome preparations may include biomarkers, such as disease biomarkers (diagnostic markers) for various disease (early stage and late stage cancers, neurological disorders (Parkinson disease, Alzheimer disease), diabetes, pancreatic diseases, renal failure, infectious diseases (HIV, tuberculosis, malaria, hepatitis)). The present methods and devices may be used to detect and monitor animals (human, live-stock, companion animal) for infectious diseases, such as tuberculosis and other diseases. The methods and devices require minimal sample material (10 μl), are rapid, economical, yield highly enriched small molecule (e.g., exosoines) preparations, and do not require electricity. 2. The particle size separation device of wherein the series of membrane filters of decreasing size comprise an 80 μm 10 μm and 0.2 μm filter membrane.3. The particle size separation device of wherein a washer is included between each membrane filter in the series of membrane filters.4. The particle size separation device of claim lwhrein the resin matrix comprises5. The particle size separation device of further defined as a separation column.6. The particle size separation devise of wherein the device removes particles having a size of greater than about 150 nm to about 200 nm from a sample.7. The particle size separation devise of wherein the sample is a body fluid.8. The particle size separation device of wherein the body fluid is blood claim 7 , saliva claim 7 , breast milk claim 7 , serum claim 7 , plasma claim 7 , or ...

FLOW-TYPE ION SELECTIVE ELECTRODE, ELECTROLYTE CONCENTRATION MEASURING DEVICE USING THE SAME, AND BIOCHEMICAL AUTOMATIC ANALYZER

There is provided an electrolyte measuring device that can decrease a liquid amount used for measurement, in which stable sealing can be provided in connecting passages of ion selective electrodes to each other with no gap, while maintaining high measurement accuracy of an existing ion selective electrode, and a residing sample liquid can be greatly decreased. In a flow-type ion selective electrode, a sealing member is used, which can be brought into intimate contact with a passage connecting unit to near a passage hole. A gap regulating member is provided to keep a gap between the electrodes constant and to prevent the sealing member from being excessively pressed. An electrode case has a structure suitable for the sealing member for allowing the alignment and holding of the sealing member. 1. A device for measuring a sample liquid using an ion selective electrode , the device comprising:an upstream side passage connection unit; anda sealing member,wherein the upstream side passage connection unit comprises:a first passage penetrated through the upstream side passage connection unit; anda passage connecting unit, configured to connect a second passage provided on the ion selective electrode,wherein the passage connecting unit includes the sealing member for sealing a gap between the first passage of the upstream side passage connection unit and the second passage of the ion selective electrode,wherein the sealing member is integrally formed of:a sheet portion having a first surface connecting the upstream side passage connection unit and a second surface configured to connect the ion selective electrode, the sheet portion having an opening; anda projection portion that projects from the sheet portion;wherein rubber hardness of a material configuring the sealing member is lower than rubber hardness of a material configuring of the upstream side passage connection unit, andwherein the upstream side passage connection unit has a recess holding the projection portion ...

SAMPLE ACQUISITION INFORMATION MANAGEMENT DEVICE, SAMPLE ACQUISITION INFORMATION MANAGEMENT SYSTEM, AND SAMPLE ACQUISITION INFORMATION MANAGEMENT METHOD

There are provided a sample acquisition information management device, a sample acquisition information management system, and a sample acquisition information management method that allow a subject who is to collect a sample by oneself to reliably perform a sample acquisition operation. Each of the sample acquisition information management device and the sample acquisition information management system includes an image acquisition unit () that acquires an image and a video of a sample acquisition operation of a subject using a sample examination kit, an output unit () that outputs acquisition means information and operation check information, and a processing unit () that determines whether or not the image or the video acquired by the image acquisition unit () corresponds to a correct sample acquisition operation and controls display of the acquisition means information and the operation check information. Further, the sample acquisition information management method uses the sample acquisition information management system. 1. A sample acquisition information management device comprising:an input-output interface configured to acquire at least one of an image or a video of a sample acquisition operation of a subject using a sample examination kit, and output acquisition means information and operation check information; andat least one processor configured to determine whether or not the image or the video acquired by the input-output interface corresponds to a correct sample acquisition operation, and control display of the acquisition means information and the operation check information,wherein the at least one processor is further configured to:control the input-output interface to deliver the acquisition means information according to a request of the subject, stop delivering the acquisition means information in a case where delivery of a predetermined sample acquisition operation of the acquisition means information ends, and deliver the operation check ...

METHOD OF DETECTING PRESENCE OR ABSENCE OF A CLOT IN A LIQUID SAMPLE ANALYZER

The invention relates to a method of detecting a clot in a measurement chamber of a liquid sample analyzer, wherein the liquid sample analyzer comprises at least two analyte sensors, a first analyte sensor, for measuring a first analyte in a liquid sample, and one or more second analyte sensors, for measuring one or more second analytes in the liquid sample in the measurement chamber, the method comprising the steps of, (a) at least partly filling the measurement chamber with a known solution having a composition comprising the first analyte at a pre-determined level, and the second one or more analytes at pre-determined levels, (b) obtaining a first sequence of measurement results by the first analyte sensor, and simultaneously obtaining a second sequence of measurement results by the second, one or more analyte sensors, (c) determining a change of the first sequence of measurement results, (d) determining a change of the second one or more sequence of measurement results, (e) comparing the change of the first sequence of measurement results with the second sequence of measurement results, (f) determining presence or absence of a clot in the measurement chamber based on said comparison. 1. A method of detecting a clot in a measurement chamber of a liquid sample analyzer , wherein the liquid sample analyzer comprises at least two analyte sensors , a first analyte sensor , for measuring a first analyte in a liquid sample , and one or more second analyte sensors , for measuring one or more second analytes in the liquid sample in the measurement chamber , the method comprisinga. at least partly filling the measurement chamber with a known solution having a composition comprising the first analyte at a pre-determined level, and the second one or more analytes at pre-determined levels,b. obtaining a first sequence of measurement results by the first analyte sensor, and simultaneously obtaining a second sequence of measurement results by the second, one or more analyte ...

METHOD AND DEVICE FOR DETECTING ENDOTOXIN

Endotoxin is detected by electrochemical measurement. A detection device of endotoxin detects endotoxin contained in a fluid to be tested (for example, a dialysate) specifically in the following manner. First, the fluid to be tested is brought into contact with an adsorbent that adsorbs endotoxin to cause the adsorbent to adsorb the endotoxin. Then, a basic solution is brought into contact with the adsorbent to desorb the endotoxin. Electrochemical measurement of the basic solution containing the desorbed endotoxin is performed. The endotoxin can be detected by the electrochemical measurement with high sensitivity at low cost. 1. A method for detecting endotoxin by electrochemical measurement , characterized by comprising the steps of:bringing a fluid to be tested into contact with an adsorbent that adsorbs endotoxin to adsorb the endotoxin;bringing a basic solution into contact with the adsorbent having adsorbed the endotoxin to desorb the endotoxin from the adsorbent; andelectrochemically measuring the basic solution containing the desorbed endotoxin.2. The method for detecting endotoxin according to claim 1 , characterized in that the fluid to be tested is a dialysate claim 1 , and the adsorbent is washed with washing water before the basic solution is brought into contact with the adsorbent having adsorbed the endotoxin.3. A device for detecting endotoxin by electrochemical measurement claim 1 , characterized by comprising:an adsorbent that adsorbs endotoxin;supply means for supplying a basic solution for desorbing the endotoxin; andmeasurement means for performing electrochemical measurement,wherein a fluid to be tested is brought into contact with the adsorbent to adsorb the endotoxin, then the basic solution is brought into contact with the adsorbent to desorb the endotoxin, and the basic solution containing the desorbed endotoxin is electrochemically measured. The present invention relates to a method and a device for detecting endotoxin, and more ...

Wafer level sequencing flow cell fabrication

A method for forming sequencing flow cells can include providing a semiconductor wafer covered with a dielectric layer, and forming a patterned layer on the dielectric layer. The patterned layer has a differential surface that includes alternating first surface regions and second surface regions. The method can also include attaching a cover wafer to the semiconductor wafer to form a composite wafer structure including a plurality of flow cells. The composite wafer structure can then be singulated to form a plurality of dies. Each die forms a sequencing flow cell. The sequencing flow cell can include a flow channel between a portion of the patterned layer and a portion of the cover wafer, an inlet, and an outlet. Further, the method can include functionalizing the sequencing flow cell to create differential surfaces.

APPARATUS, SYSTEM, AND METHOD FOR COLLECTING A TARGET MATERIAL

This disclosure is directed to an apparatus, system and method for retrieving target material from a suspension. A system includes a processing vessel, a displacement fluid, and a tube. The tube includes a funnel, a cannula, and a cavity. The cannula allows for fluid communication between the funnel and the cavity, such that the processing vessel is inserted into the cavity, and the cannula extends into the processing vessel. 1. A system comprising: a first end,', 'a second end comprising an aperture,', 'a biological suspension comprising a target material, and', a funnel comprising a mouth proximal to the first end and an apex distal to the first end,', 'a cavity extending from the aperture in the second end towards the apex of the funnel, and', 'a cannula extending from the apex of the funnel into the cavity; and, 'a collection segment between the first and second ends, the collection segment comprising'}], 'a tube comprising'}a processing vessel comprising a closed end and a displacement fluid having a density greater than a density of the target material,wherein at least a portion of the processing vessel is located within the cavity of the tube, andwherein the cannula extends through the closed end of the processing vessel.2. The system of claim 1 , wherein the displacement fluid is selected from the group consisting of: a solution of colloidal silica particles coated with polyvinylpyrrolidone claim 1 , a polysaccharide solution claim 1 , iodixanol claim 1 , an organic solvent claim 1 , a liquid wax claim 1 , an oil claim 1 , a gas claim 1 , olive oil claim 1 , mineral oil claim 1 , silicone oil claim 1 , immersion oil claim 1 , mineral oil claim 1 , paraffin oil claim 1 , silicon oil claim 1 , fluorosilicone claim 1 , perfluorodecalin claim 1 , perfluoroperhydrophenanthrene claim 1 , perfluorooctylbromide claim 1 , organic solvents claim 1 , 1 claim 1 ,4-Dioxane claim 1 , acetonitrile claim 1 , ethyl acetate claim 1 , tert-butanol claim 1 , cyclohexanone claim ...

Apparatus and method for separating and concentrating a component of a fluid

A system for separating and concentrating a component of a whole material is disclosed. The whole material can include a material that has more than one component, such as whole blood that can include at least red blood cells, monocytes, and plasma. The system can include a substantially single container including a separation section and a concentration section wherein a component can be moved from the separation section, after a separation, to the concentration section to be concentrated. The concentrated component can then be withdrawn from the separation and concentration container for a selected procedure.

ADHERENT CANCER CELL LINE EXPRESSING A HEMATOLOGICAL TUMOR ANTIGEN

The present invention relates to a transduced cancer cell line stably expressing a leukemia tumor antigen, wherein the cancer cell line is cervical cancer cells, breast cancer cells, ovarian cancer cells, pancreatic cancer cells, lung cancer cells, or glioblastoma cells. The transduced adherent cell line of the present invention is useful for many pre-clinical applications such as real time cytotoxicity assay or to test the effects of CAR-T cells that target the tumor antigen. The present invention is exemplified by Hela cell line stably expressing CD19. 1. A method for measuring cytotoxicity of CD19-CAR T cells , comprising:seeding transduced Hela cancer cells that stably express CD19 on a plate,adding CD19-CAR T cells to the plate, andmeasuring real time cytotoxicity of the CD19-CAR T cells.2. The method of claim 1 , wherein the CAR comprises CD19 scFv claim 1 , CD28-transmembrane domain claim 1 , CD28 activation domain claim 1 , and CD3 zeta.3. The method of claim 1 , wherein the CAR further comprises a FLAG tag. This application is a continuation of U.S. application Ser. No. 15/610,419, filed May 31, 2017, which claims the priority of U.S. Provisional Application No. 62/343,976, dated Jun. 1, 2016. The above applications are incorporated herein by reference in their entireties.The Sequence Listing is concurrently submitted herewith with the specification as an ASCII formatted text file via EFS-Web with a file name of Sequence Listing.txt with a creation date of May 15, 2017, and a size of 13.0 kilobytes. The Sequence Listing filed via EFS-Web is part of the specification and is hereby incorporated in its entirety by reference herein.The present invention relates to Hela-CD19 cell line that stably express CD19 (cluster of differentiation 19), which is a marker of hematopoietic cancers.Hela cell line was derived from cervical cancer cells taken on Feb. 8, 1951 from Henrietta Lacks, who died of cancer on Oct. 4, 1951 [Ghorashian et al. 2015, 169, 463-478.] The cell ...

Apparatus and method for separating plasma from blood and delayed wetting

Devices and methods are disclosed herein for separating a supernate from a suspension. The apparatus consists of a sample zone, a controllable gate, and an analysis zone. The sample zone holds the suspension. The analysis zone passively transports a supernate formed from the suspension by capillary transport. A controllable gate prevents the suspension in the sample zone from flowing into the analysis zone. The controllable gate can be triggered after the supernate has separated from the suspension to allow the supernate to flow into the analysis zone.

PARTICLE SEPARATING AND MEASURING DEVICE, AND PARTICLE SEPARATING AND MEASURING APPARATUS

A particle separating and measuring device of the present disclosure includes: a first flow path device including a post-separation flow outlet through which a first fluid containing specific particles to be separated flows out; and a second flow path device on which the first flow path device is placed and including a first flow inlet through which the first fluid flows in, the first flow path device in which the post-separation flow outlet is arranged in a lower surface is placed on the second flow path device in which the first flow inlet is arranged in an upper surface of a first region, the post-separation flow outlet and the first flow inlet are connected so as to face each other, and a size of an opening of the first flow inlet is larger than a size of an opening of the post-separation flow outlet. 1. A particle separating and measuring device comprising:a first flow path device having a plate-like shape and including a pre-separation flow inlet through which a fluid flows in that contains specific particles to be separated, a main flow path connected to the pre-separation flow inlet, a plurality of branch flow paths each connected to the main flow path, and a post-separation flow outlet through which a first fluid flows out that contains the specific particles that have been separated; anda second flow path device having a plate-like shape and having a first region on which the first flow path device is placed, and a second region that serves as a measurement region for the specific particles, the second flow path device including a first flow inlet through which the first fluid flows in, a second flow inlet through which a second fluid not containing the specific particles flows in, a first flow path connected to the first flow inlet and through which the first fluid passes, and a second flow path connected to the second flow inlet and through which the second fluid passes, wherein the first flow path and the second flow path are arranged in the second ...

Modified filter membrane and the use thereof

A surface-modified filter membrane for filtering blood, in particular for separating blood plasma and blood serum, and a method for the production thereof, a filter provided therewith and the use thereof.

SYSTEM AND METHOD FOR BLOOD SAMPLE COLLECTION AND PROCESSING

Blood sample collection and processing system () using a tube () and a holder () is described herein. The tube () includes a plasma separation system () for converting a whole blood sample () into a plasma sample without using centrifugation. The plasma separation system () may comprise a filtration system such as a hollow fiber element (). The tube () is thereafter placed in the holder () for analysis regarding the plasma. The results of the analysis may be displayed to the user via a display screen () to allow the user to determine whether the sample is sufficient for further use. Inasmuch as the analysis system () performs a rapid analysis of the plasma sample, the patient may still be present or in close proximity and if the sample is insufficient, the healthcare provider can take another whole blood sample (), counsel the client regarding the blood sample (), or examine the patient further. 1. A collection system comprising:(a) a tube; and(b) a holder, wherein the holder is configured to receive the tube therein.2. The collection system of claim 1 , wherein the tube comprises a plasma separation system.3. The collection system of claim 2 , wherein the plasma separation system comprises a filtration system.4. The collection system of claim 3 , wherein the filtration system comprises a hollow fiber element.5. The collection system of claim 4 , wherein the hollow fiber element comprises:(a) a filter wall, wherein the filter wall is configured to allow plasma to pass therethrough; and(b) an interior defined by the filter wall, wherein the interior is configured to received whole blood therein.6. The collection system of claim 5 , further comprising a pressure system configured to force whole blood through the filter wall.7. The collection system of claim 1 , wherein the tube defines a collection chamber.8. The collection system of claim 7 , wherein the collection chamber defines an analysis pocket therein.9. The collection system of claim 1 , wherein the holder ...

MICROFLUIDIC DEVICE

A microfluidic device () for separating a liquid L into first and second liquid components L, Lthereof is described. The microfluidic device () comprises an inlet () for receiving the liquid therethrough. The microfluidic device () comprises a first outlet () for the first liquid component L, wherein the first outlet () is fluidically coupled to the inlet () via a first passageway (). The microfluidic device () comprises a second outlet () for the second liquid component L, wherein the second outlet () is fluidically coupled to the first passageway (A) via a first set of N conduits (A, B, C, D, E), wherein N is a positive integer greater than 1, wherein respective conduits A, B, C, D, E of the first set of N conduits divide from the first passageway A at respective divisions (A, B, C, D, E) from the inlet therealong The respective conduits A, B, C, D, E of the first set of N conduits are arranged to, at least in part, equalize flowrate ratios at the respective divisions (A, B, C, D, E). 120-. (canceled)21. A microfluidic device for separating a liquid into first and second liquid components thereof , the microfluidic device comprising:an inlet for receiving the liquid therethrough;a first outlet for the first liquid component, wherein the first outlet is fluidically coupled to the inlet via a first passageway; anda second outlet for the second liquid component, wherein the second outlet is fluidically coupled to the first passageway via a first set of N conduits, wherein N is a positive integer greater than 1, wherein respective conduits of the first set of N conduits divide from the first passageway at respective divisions from the inlet there-along, the respective conduits of the first set of N conduits are arranged to, at least in part, equalize flowrate ratios at the respective divisions;', 'the first passageway comprises a set of expansion members; and', 'respective expansion members of the set of expansion members correspond with the respective conduits of the ...

FILTER FOR FILTRATION OF NUCLEATED CELLS AND FILTRATION METHOD USING THE SAME

A filtration method for filtration of nucleated cells that includes providing a filter containing at least one of a metal and a metal oxide as a major component thereof and having a plurality of through-holes therein, and passing a liquid containing the nucleated cells through the filter. The diameter of an inscribed circle of each of the plurality of through-holes is smaller than the size of the nuclei of the nucleated cells, and the inscribed circle of each of the plurality of through-holes touches all sides defining an opening of the through-hole. 1. A method for filtration of nucleated cells , the method comprising:providing a filter comprising at least one of a metal and a metal oxide as a major component thereof and having a plurality of through-holes therein, each of the plurality of through-holes having a square shape, wherein a diameter of an inscribed circle of each of the plurality of through-holes is smaller than a size of nuclei of the nucleated cells, the inscribed circle of each of the plurality of through-holes touching all sides defining an opening of the through-hole; andpassing a liquid containing the nucleated cells through the filter.2. The filtration method according to claim 1 , wherein a ratio of the diameter of the inscribed circle of each of the plurality of through-holes to the size of the nuclei of the nucleated cells is 0.06 to 0.64.3. The filtration method according to claim 1 , wherein a ratio of the diameter of the inscribed circle of each through-hole to the size of the nuclei of the nucleated cells is 0.06 or more.4. The filtration method according to claim 1 , wherein a ratio of the diameter of the inscribed circle of each through-hole to the size of the nuclei of the nucleated cells is 0.07 or more.5. The filtration method according to claim 1 , wherein an immersion potential of one at least one of the metal and the metal oxide in phosphate-buffered saline is higher than 0.03 V with respect to a silver chloride reference electrode ...

MEASUREMENT OF SERUM LIPOPROTEINS

Although a more accurate estimate of a person's risk of cardiovascular disease can be made on the basis of the number of lipoprotein particles per unit volume in the person's blood, current methods all rely on measuring the mass of lipoprotein cholesterol per unit volume. It has been discovered that a rapid and accurate lipoprotein particle count can be obtained by photometry. A method and apparatus are provided for measuring the number of lipoprotein particles in a sample using photometry. 1. A method of measuring lipoprotein particle count in a sample from a subject , the method comprising:a. introducing into an apparatus for measuring the lipoprotein particle number, a plurality of lipoprotein fractions comprising a LDL fraction, a HDL fraction, and a VLDL fraction, wherein each of the plurality of lipoprotein fractions are separated from one another;b. obtaining a photometric measurement in at least the LDL fraction; andc. calculating a particle count for the LDL fraction that is a function of the photometric measurement,wherein separation of the HDL fraction and the VLDL fraction is maintained during steps (a) to (c).2. The method of further comprising:a. obtaining a photometric measurement in the HDL fraction; andb. calculating a particle count for the HDL fraction that is a function of the photometric measurement.3. The method of further comprising:a. obtaining a photometric measurement in at least the VLDL fraction; andb. calculating a particle count for the VLDL fraction that is a function of the photometric measurement.4. The method of further comprising:a. obtaining a photometric measurement in at least the HDL fraction;b. calculating a particle count for the HDL fraction that is a function of the photometric measurement;c. obtaining a photometric measurement in at least the VLDL fraction; andd. calculating a particle count for the VLDL fraction that is a function of the photometric measurement.5. The method of claim 1 , wherein the plurality of ...

Plasma Separation Device

Devices and methods are provided that permit efficient and selective separation of liquid biological specimens into at least two constituent components to facilitate subsequent quantitative and qualitative analysis on at least one analyte of interest in at least one of the components. The devices generally include one or more sample deposition regions supported on a base. Each sample deposition region includes a separation membrane for separating the liquid biological specimen into two different fractions. The first fraction is trapped by the separation membrane while the second fraction passes through the separation membrane and into a respective collection membrane. The separation and collection membranes are easily separable from the devices and can be utilized for further processing and analysis.

Capillary fluid flow measurement and capillary flow device therefore

The present invention relates to a liquid flow device, in particular a capillary testing device provided as a chip, comprising a second pathway which intersects the first pathway at a downstream point of convergence, so that the two pathways share an outlet and when liquid in the second pathway reaches the point of convergence, liquid flow in the first pathway stops. Means for measuring the distance travelled by liquid in the first pathway are provided to determine the extent of liquid flow and to enable correlation with the amount of analyte in the liquid.

APPARATUS AND METHOD FOR SEPARATING AND CONCENTRATING FLUIDS CONTAINING MULTIPLE COMPONENTS

An apparatus that allows for separating and collecting a fraction of a sample. The apparatus, when used with a centrifuge, allows for the creation of at least three fractions in the apparatus. It also provides for a new method of extracting the buffy coat phase from a whole blood sample. A buoy system that may include a first buoy portion and a second buoy member operably interconnected may be used to form at least three fractions from a sample during a substantially single centrifugation process. Therefore, the separation of various fractions may be substantially quick and efficient. 132-. (canceled)33. A method of isolating a fraction of nucleated cells , comprising:obtaining a volume of whole blood from a patient;obtaining a volume of bone marrow from a patient;loading the volume of whole blood and the volume of bone marrow into a container configured to be placed in a centrifuge, the container comprising a fraction separator having a selected density operable to physically separate fractions of whole blood based on the density of the fractions, the fractions including a fraction comprising the nucleated cells;centrifuging the container, so as to create a fraction comprising nucleated cells; andcollecting the fraction comprising the nucleated cells.34. The method according to claim 33 , wherein the fraction comprising nucleated cells comprises cells selected from the group consisting of platelets claim 33 , white blood cells claim 33 , stem cells claim 33 , and mixtures thereof.35. The method according to claim 33 , wherein the centrifuging creates at least three distinct fractions comprising a red blood cell fraction claim 33 , a buffy coat fraction claim 33 , and a plasma fraction.36. The method according to claim 33 , wherein the buoy is configured to have a density less than the density of red blood cells and a density greater than the density of the fraction comprising nucleated cells.37. The method according to claim 33 , wherein the separator further ...

SYSTEM AND METHOD FOR A BIOMIMETIC FLUID PROCESSING

A system and method are provided for harvesting target biological substances. The system includes a substrate and a first and second channel formed in the substrate. The channels longitudinally extending substantially parallel to each other. A series of gaps extend from the first channel to the second channel to create a fluid communication path passing between a series of columns with the columns being longitudinally separated by a predetermined separation distance. The system also includes a first source configured to selectively introduce into the first channel a first biological composition at a first channel flow rate and a second source configured to selectively introduce into the second channel a second biological composition at a second channel flow rate. The sources are configured to create a differential between the first and second channel flow rates to generate physiological shear rates along the second channel that are bounded within a predetermined range. 1. A biomimetic microfluidic system comprising:a substrate having a first portion and a second portion;a first channel formed in the first portion of the substrate, the first channel extending from a first input to a first output along a longitudinal dimension, the first channel configured to selectively receive at least one first biological composition at a first channel flow rate, the at least one first biological composition including a biological source material;a second channel formed in the second portion of the substrate, the second channel extending from a second input to a second output along the longitudinal dimension, the second channel configured to selectively receive at least one second biological composition at a second channel flow rate, wherein at least a portion of the first and second channels are substantially parallel;microchannels extending between the first channel and the second channel to create a fluid communication path between the first and second channels, wherein the ...

BLOOD CELL COUNTING DEVICE AND METHOD

A device for use in imaging a liquid sample comprises an inlet for accepting the sample, a connection conduit and a detection chamber for detection of the sample, preferably optical detection of the sample. The connection conduit connects the inlet to the detection chamber and contains one or more dry reagents for reaction with the sample as the sample passes through the connection conduit. Specific embodiments include devices arranged for treating a blood sample, in particular lysing and staining the sample. The liquid flow may be driven by capillary effect. The device may further include liquid handling structures arranged for centrifugally driven liquid flow, for example to meter a volume of sample and separate the sample into phases by centrifugation. 1. A device for use in imaging a liquid sample , the device comprising:an inlet for accepting a sample into the device;a connection conduit;a detection chamber for optical detection of the sample,wherein the connection conduit connects the inlet to the detection chamber, andwherein the connection conduit contains one or more dry reagents for reaction with the sample as the sample passes through the connection conduit.2. The device of claim 1 , wherein the sample is a blood sample claim 1 , the one or more dry reagents include a haemolysing agent for selective lysis of erythrocytes in the blood sample and a staining agent for selectively staining leukocytes in the blood sample.3. The device of or claim 1 , wherein the connection conduit comprises a main conduit portion and one or more protrusions extending from the main conduit portion wherein the dry reagents are stored in the one or more protrusions and respective junction regions between the one or more protrusions and the main conduit portion provide a reaction region in which gradual resuspension of the dry reagents can occur.4. The device of claim 3 , wherein the protrusions comprise a main portion and a neck portion in the region of the junction claim 3 , the ...

SYSTEMS, DEVICES, AND METHODS FOR BODILY FLUID SAMPLE COLLECTION, TRANSPORT, AND HANDLING

Bodily fluid sample collection systems, devices, and method are provided. The sample is collected at a first location and subjected to a first sample processing step. The sample may be shipped to a second location and subjected to a second sample processing step that does not introduce contaminants into a plasma portion of the sample formed from the first processing step. 1. A method for use with a bodily fluid sample from a subject , the method comprising:shipping a plurality of sample containers from a first location to a second location, wherein each of said sample containers contains about 500 μL or less but greater than about 30 μL of the bodily fluid sample, wherein interior volume of each of the sample containers is about 600 μL or less,subjecting the bodily fluid sample to a first accelerated sedimentation force of at least about 1400 g or greater to form a first processed sample;shipping the first processed sample from a first location to a second location; andsubjecting the first processed sample at the second location to a second accelerated sedimentation force of greater than about 10 g but less than about 500 g.2. (canceled)3. The method of wherein the first processed sample contains a heparin-based anti-coagulant.4. The method of wherein the first processed sample contains a plasma portion separated by a separation gel from a formed-blood component portion.5. The method of wherein the second processed sample does not force electrolytes though the separation gel from the formed-blood component portion to the plasma portion.6. The method of wherein the second processed sample does not force liquid though the separation gel from the formed-blood component portion to the plasma portion.7. The method of wherein the first accelerated sedimentation force is provided through centrifugation.8. The method of wherein the second accelerated sedimentation force is provided through centrifugation.9. The method of wherein a plurality of samples are shipped from a ...

SYSTEM FOR MEASURING TOTAL HEMOGLOBIN IN BLOOD AND METHOD OF DOING THE SAME

A system can be used for determining a total hemoglobin concentration in a blood sample. The system has a strip that has a capillary channel having an entrance and a vent. A disposable body is housed within the capillary channel and adapted to receive a flow of blood from the entrance such that air in the disposable body is pushed out through a vent. A measurement system is configured to measure a property of the flow of blood. A non-transitory computer readable medium stores a program in memory that causes a processor to execute a process for determining the total hemoglobin concentration in the blood sample. The process involves receiving a first measurement from the measurement system. Then, calculating the total hemoglobin concentration in the blood sample based on the property. Finally, displaying the total hemoglobin concentration in the blood sample. 1. A system for determining a total hemoglobin concentration in a blood sample; the system comprising:a strip further comprising: a capillary channel having an entrance and a vent;a disposable body, housing the capillary channel and adapted to receive a flow of blood from the entrance such that air in the disposable body is pushed out through a vent;a measurement system configured to measure a property of the flow of blood; receiving a first measurement from the measurement system;', 'calculating the total hemoglobin concentration in the blood sample based on the property;', 'displaying the total hemoglobin concentration in the blood sample., 'a non-transitory computer readable medium, storing a program in memory that causes a processor to execute a process for determining the total hemoglobin concentration in the blood sample, the process comprising2. The system of claim 1 , further comprising: diode for optical detection connected to the measurement system; and a radiation emitter which produces radiation that travels through the disposable body claim 1 , the channel claim 1 , the blood and into the diode for ...

CAPTURE, IDENTIFICATION AND USE OF A NEW BIOMARKER OF SOLID TUMORS IN BODY FLUIDS

A new sensitive cell biomarker of solid tumors is identified in blood. This biomarker can be used to determine presence of solid tumors, rapid determination of treatment response, early detection of cancer, early detection of cancer recurrence, and may be used to determine therapy. 1. A method of screening a subject for cancer , comprising detecting circulating Cancer Associated Macrophage-Like cells (CAMLs) in blood of a subject , wherein said detecting comprises:(a) isolating intact cells of between 20 and 300 micron in size from a biological sample obtained from a subject using a means selected from the group consisting of size exclusion methodology, immunocapture, red blood cell lysis, white blood cell depletion, FICOLL separation, electrophoresis, dielectrophoresis, flow cytometry, magnetic levitation, microfluidic chip, and a combination thereof, and (i) a large atypical polyploid nucleus of about 14-64 μm in size or multiple nuclei, and', '(ii) a morphological shape selected from the group consisting of spindle, tadpole, round, oblong, two legs, more than two legs, thin legs, and amorphous,, '(b) selecting cells isolated in (a) having'}thereby detecting CAMLs in a biological sample from a subject,wherein when CAMLs are detected in the biological sample, the subject is determined to have cancer.2. The method of claim 1 , wherein the cancer is carcinoma or solid tumor.3. The method of claim 1 , wherein the cancer is breast claim 1 , prostate claim 1 , lung claim 1 , pancreatic claim 1 , or colorectal.4. The method of claim 1 , further comprising detecting circulating tumor cells (CTCs) in the biological sample.5. The method of claim 1 , wherein the cells are isolated in (a) using size exclusion methodology.6. The method of claim 5 , wherein the size exclusion methodology is use of a microfilter having pores ranging in size from 5 to 20 microns.7. The method of claim 1 , wherein the blood is peripheral blood.8. A method for confirming a diagnosis of cancer in a ...

METHOD OF DETECTING PRESENCE OR ABSENCE OF A CLOT IN A LIQUID SAMPLE ANALYZER

The disclosure relates to a method of detecting a clot in a measurement chamber of a liquid sample analyzer, wherein the liquid sample analyzer comprises at least two analyte sensors, a first analyte sensor, for measuring a first analyte in a liquid sample, and one or more second analyte sensors, for measuring one or more second analytes in the liquid sample in the measurement chamber, the method comprising the steps of, (a) at least partly filling the measurement chamber with a known solution having a composition comprising the first analyte at a pre-determined level, and the second one or more analytes at pre-determined levels, (b) obtaining a first sequence of measurement results by the first analyte sensor, and simultaneously obtaining a second sequence of measurement results by the second, one or more analyte sensors, (c) determining a change of the first sequence of measurement results, (d) determining a change of the second or more sequence of measurement results, and (e) comparing the change of the first sequence of measurement results with the second sequence of measurement results. 1. A method of detecting a clot in a measurement chamber of a liquid sample analyzer , wherein the liquid sample analyzer comprises at least two analyte sensors , a first analyte sensor , for measuring a first analyte in a liquid sample , and one or more second analyte sensors , for measuring one or more second analytes in the liquid sample in the measurement chamber , the method comprising the steps of;a. at least partly filling the measurement chamber with a known solution having a composition comprising the first analyte at a pre-determined level, and the second one or more analytes at pre-determined levels,b. obtaining a first sequence of measurement results by the first analyte sensor, and simultaneously obtaining a second sequence of measurement results by the second, one or more analyte sensors;c. determining a change of the first sequence of measurement results;d. ...

Measuring Multi-Analyte Samples Using an In-Line Flow Cell

Methods and systems for analyzing ratios of analytes within a flowing sample are provided. The flowing sample can be processed in real-time to determine a time interval over which a predetermined amount of a group of analytes passes by a fixed point in a flow cell. The predetermined amount can be routed to a sample container for future processing. The sample can comprise diluted blood and the analytes can comprise a component of hemoglobin, such as A1c, and the total amount of hemoglobin, of which the predetermined amount is metered. 1. A method for measuring analytes in a sample , the method comprising:flowing a sample containing analytes through a detector;measuring a measurand of the analytes passing through the detector;determining a time interval over which the measurand reaches a predetermined quantity; anddiverting a portion of the flowing sample containing the predetermined quantity of the measurand to a sample volume.2. The method of claim 1 , wherein the measurand has a linear relationship with analyte concentration.3. The method of claim 2 , wherein the measurand is based on light absorbance of analyte.4. The method of claim 1 , wherein measuring comprises performing a real-time integration of the measurand over the time interval.5. The method of claim 4 , wherein the time interval is pre-determined.6. The method of claim 4 , wherein the time interval starts at a first time point that is determined by a first measured threshold of the measurand.7. The method of claim 6 , wherein the sample is flowed into a waste output before the first time point.8. The method of claim 7 , wherein the first time point is the basis to switch at least one valve for diverting the portion of the flowing sample from the waste output to the sample volume.9. The method of claim 4 , wherein the time interval ends at a second time point that is determined by a second measured threshold of the measurand.10. The method of claim 9 , wherein the second threshold is determined by a ...

METHOD OF PREDICTING PATIENT PROGNOSIS USING RARE CELLS

The purpose of the present invention is to provide a method for accurately predicting a cancer patient prognosis based on a count of desired cells for which expression of a leukocyte marker and an epithelial marker is hardly exhibited by detecting those cells. Provided is a method for diagnosing an overall survival prognosis for a patient suffering from cancer, the method including: a step of obtaining a concentrated solution containing desired cells by pre-treating a biological sample obtained from the patient; a step of optically detecting the concentrated cells; and a step of detecting the desired cells from the detected image, wherein an association is made with the overall survival prognosis diagnosis by counting the detected desired cells, and wherein the desired cells are cells confirmed by the existence of a cell nucleus and in which expression of a leukocyte marker and an epithelial marker is hardly exhibited. 1. A method of determining overall survival prognosis for a patient suffering from cancer , comprising steps of:1) obtaining a concentrated liquid by concentrating target cells from an organism sample obtained from the patient;2) optically detecting and counting cells contained in the concentrated liquid, the cells each having cell nucleus and not substantially expressing a leukocyte marker and an epithelial marker; and3) associating the number of counted target cells and overall survival prognosis.2. The method according to claim 1 , wherein the leukocyte marker is CD45 claim 1 , and the epithelial marker is CK.3. The method according to claim 1 , wherein the target cells have diameters of not less than 10 μm.4. The method according to claim 1 , wherein the target cells are circulating tumor cells (CTCs).5. The method according to claim 1 , wherein the step of obtaining a concentrated liquid is a concentration method based on a specific gravity difference of a biological sample.6. The method according to claim 1 , wherein the pretreatment includes ...

Apparatus, system, and method for collecting a target material

This disclosure is directed to an apparatus, system and method for retrieving a target material from a suspension. A system includes a plurality of processing vessels and a collector. The collector funnels portions of the target material from the suspension through a cannula and into the processing vessels. Sequential density fractionation is the division of a sample into fractions or of a fraction of a sample into sub-fractions by a step-wise or sequential process, such that each step or sequence results in the collection or separation of a different fraction or sub-fraction from the preceding and successive steps or sequences. In other words, sequential density fractionation provides individual sub-populations of a population or individual sub-sub-populations of a sub-population of a population through a series of steps.

SAMPLE ANALYZING APPARATUS, DISEASE MONITORING SYSTEM, AND METHOD FOR MANAGING DATA OF SAMPLE ANALYZING APPARATUS

A sample analyzing apparatus comprising a measurement unit configured to measure samples obtained from subjects, an obtaining unit configured to obtain the presence or absence of a disease regarding each sample, a memory in which a measurement result obtained by the measurement unit and the presence or absence of the disease obtained by the obtaining unit are stored in association with the sample, and a communication unit configured to transmit disease information based on the presence or absence of the disease stored in the memory to a receiving apparatus installed in an external facility. 1. A sample analyzing apparatus comprising:a measurement unit configured to measure samples obtained from subjects;an obtaining unit configured to obtain the presence or absence of a disease regarding each sample;a memory in which a measurement result obtained by the measurement unit and the presence or absence of the disease obtained by the obtaining unit are stored in association with the sample; anda communication unit configured to transmit disease information based on the presence or absence of the disease stored in the memory to a receiving apparatus installed in an external facility.2. The sample analyzing apparatus of claim 1 , whereinthe obtaining unit comprises an input unit for inputting the presence or absence of the disease.3. The sample analyzing apparatus of claim 1 , whereinthe obtaining unit comprises a determining unit configured to determine the presence or absence of the disease regarding the sample, based on the measurement result obtained by the measurement unit.4. The sample analyzing apparatus of claim 3 , whereinthe obtaining unit comprises an input unit for inputting the presence or absence of another disease different from the disease.5. The sample analyzing apparatus of claim 1 , whereinthe communication unit transmits the disease information to the receiving apparatus, every time a predetermined period has elapsed.6. The sample analyzing apparatus of ...

Blood separation container for extracting self-platelet

The present invention relates to a blood separation container for extracting self-platelet. According to the present invention, since a second coupling portion is formed at a release prevention member coupled to a lower portion of a main body in a PRP separation container, and an ascending member having a bolt structure is screwed to the second coupling portion to ascend a lower cover disposed in a lower fluid chamber of the main body, a separate second main body is unnecessary unlike the related art. Therefore, the PRP separation container is easy to carry, manufacturing costs can be reduced to thereby ensure competitive price, and a PRP can be easily separated and extracted.

Separation device for use in the separation, characterization and/or identification of microorganisms

The present invention is directed to a separation device or container that can be used in the separation, isolation or pelleting of microorganisms from a test samples known to contain or suspected of containing said microorganisms. Subsequently, the separated, isolated or pelleted microorganism sample can undergo one or more interrogation steps to provide measurements useful for the characterization and/or identification of microorganism. In one aspect of the present invention, the interrogation steps can occur in situ in the separation device or container described herein.

BLOOD AND BIOLOGICAL SAMPLE COLLECTION DEVICE AND METHOD

Specially designed collection strips and their processing. By using specially designed collection strips, having a backer and one or more absorbent pads, in conjunction with a unique processing method, the processes of analyzing biological samples such as blood, or the like, may be done efficiency with the elimination of cross contamination risk. Identification of the sample stays with the sample throughout the process as it resides on the collection strip. The strip absorbs a known volume. The sample with identification is placed directly in an elution solution, without mechanically separating the sample from its identification information. Elimination of the need for mechanical separation tends to reduce cross contamination, as well as reducing sample processing time. 1. A blood collection device that does not require punching , or folding to obtain a sample for processing comprising:a flat rectangular elongated backer having a first end and a second end;a planar label area disposed on the first end upon which identifying information of a collected sample is recorded; anda separate absorbent pad attached at the second end of the flat rectangular elongate backer that does not require punching to obtain a sample whereby a blood sample is applied to the absorbent pad and dried for later testing, the construction of the blood collection device not requiring a folding, a punching out, or detachment of a portion of the blood collection device containing the blood sample, and whereby the identifying information of the collected sample stays affixed to the sample during processing of the sample.2. The blood collection device of claim 1 , in which the absorbent pad is cellulose.3. The blood collection device of claim 1 , in which the absorbent pad is polyester.4. The blood collection device of claim 1 , in which the absorbent pad is glass fiber.5. The blood collection device of claim 1 , in which the absorbent pad is treated with a detergent.6. The blood collection device ...

Sample handling device

A sample handling device includes a reservoir for holding a fluid medium. A channel system used in connection with the reservoir includes a dilution portion for a sample to be analyzed with a measurement device. The sample is arranged to be transferred from the dilution portion to the measurement device by the fluid medium. A set of capillary channels in the dilution portion is arranged to be filled by capillary action to collect an established quantity of the sample to be diluted by the fluid medium. A pump transfers the fluid medium from the reservoir to the channel system. The pump includes at least one plunger, a seal separating the reservoir and the channel system and a delivery system of potential energy including a compressible element configured to provide repeatable transfer of the fluid medium from the reservoir to the channel system.

MICROFILTRATION DEVICE

A microfiltration device comprises a substrate having a first surface and a second surface opposite to the first surface. The substrate includes a cavity between the first surface and the second surface. The substrate further includes a microfilter including a frame part in contact with the substrate and a filter part abutting the cavity. The microfilter comprises in both the frame part and the filter part a semiconducting or conducting material. 1. A microfiltration device , comprisinga substrate having a first surface and a second surface opposite to the first surface, the substrate including a cavity between the first surface and the second surface, anda microfilter including a frame part in contact with the substrate and a filter part abutting the cavity, the microfilter comprising in both the frame part and the filter part a semiconducting or conducting material.2. The microfiltration device of claim 1 , wherein the microfilter comprises a first layer of the semiconducting or conducting material.3. The microfiltration device of claim 2 , wherein the microfilter further comprises a second layer of an insulating material.4. The microfiltration device of claim 3 , wherein the first layer of the semiconducting or conducting material is a patterned layer on the second layer of the insulating material.5. The microfiltration device of claim 3 , wherein the microfilter further comprises a third layer of an insulating material claim 3 , the first layer being sandwiched between the second and the third layer.6. The microfiltration device of claim 1 , wherein the substrate includes an insulating material.7. The microfiltration device of claim 1 , wherein the microfilter and the substrate comprise a same material.8. The microfiltration device of claim 1 , wherein the microfilter is bonded on the substrate.9. The microfiltration device of claim 1 , wherein the semiconducting or conducting material comprises at least one of polysilicon claim 1 , silicon claim 1 , porous ...

BLOOD STATE ANALYSIS DEVICE, BLOOD STATE ANALYSIS SYSTEM, BLOOD STATE ANALYSIS METHOD, AND STORAGE DEVICE

A blood state analysis device () that analyzes a state of a blood sample, as used for clotting time testing, said blood sample comprising a plasma and at least one reagent, including a correction unit () configured to correct a measured blood coagulation evaluation result of the blood sample based on a relation between reference concentrations of the at least one reagent in plasma and reference blood coagulation evaluation results, the at least one reagent is an anticoagulation treatment releasing agent, a coagulation activator, an anticoagulant, a platelet activator, an antiplatelet drug, or a combination thereof and is present in the blood sample. For instance, such device () allows to correct blood clotting time results with respect to concentrations of an anti-coagulant drug present in the blood sample. 1. A blood state analysis device that analyzes a state of a blood sample comprising a plasma and at least one reagent , the device comprising:a correction unit configured to correct a measured blood coagulation evaluation result of the blood sample based on a relation between reference concentrations of the at least one reagent in plasma and reference blood coagulation evaluation results, wherein the at least one reagent is an anticoagulation treatment releasing agent, a coagulation activator, an anticoagulant, a platelet activator, an antiplatelet drug, or a combination thereof and is present in the blood sample.2. The blood state analysis device according to claim 1 , wherein the correction unit is further configured to correct the measured coagulation evaluation result to a corrected coagulation evaluation result that corresponds to a reference concentration of the at least one reagent in plasma that is substantially equal to a measured concentration of the at least one reagent in the plasma of the blood sample.3. The blood state analysis device according to claim 1 , wherein the correlation is predetermined.4. The blood state analysis device according to ...

PLATELET TEST CHIP

Disclosed is a platelet-testing chip. The platelet-testing chip includes a plurality of sample chambers storing blood samples, stirrers provided in the sample chambers to apply shearing force to the blood samples, a plurality of waste sample chambers provided so as to correspond to the plurality of the sample chambers, microchannels, through which the sample chambers and the waste sample chambers corresponding to each other are independently connected to thus form paths through which the blood samples flow from the sample chambers to the corresponding waste sample chambers, and microbeads which are received in one or more of the plurality of the sample chambers and which are coated with a reagent for activating platelets on an outer surface thereof. When the blood samples are transferred from the sample chambers through the microchannels, the microbeads are transferred together with the blood samples. 1. A platelet-testing chip comprising:a plurality of sample chambers storing blood samples;stirrers provided in the sample chambers, respectively, to stir the blood samples;a plurality of waste sample chambers provided so as to correspond to the plurality of the sample chambers;microchannels, through which the sample chambers and the waste sample chambers corresponding to each other are independently connected to thus form paths through which the blood samples flow from the sample chambers to the corresponding waste sample chambers;one or more clogging-induction channel units formed on the paths of the microchannels; andmicrobeads which are received in one or more of the plurality of the sample chambers and which are coated with a reagent for activating platelets on an outer surface thereof,wherein when the blood samples are transferred from the sample chambers through the microchannels, the microbeads are transferred together with the blood samples, thus inducing clogging of the clogging-induction channel units with the blood samples.2. The platelet-testing chip of ...

Analytical Tool and Analytical System

An analytical tool is provided for analysis of a sample by capillary electrophoresis. The analytical tool includes an inlet reservoir into which a sample is introduced, a capillary tube in communication with the inlet reservoir, a filter through which a liquid from the inlet reservoir passes, an enlarged portion undergoing a sudden increase in a cross-sectional area and being in communication with the inlet reservoir and the capillary tube, and a pressure fluctuation reducer for preventing pressure fluctuation at the enlarge portion from affecting a liquid in the capillary tube. 114-. (canceled)15. An analytical tool for analysis of a sample by capillary electrophoresis , the analytical tool comprising:{'claim-text': ['a secondary reservoir having an opening at a bottom of the secondary reservoir;', 'a connection channel connecting the inlet reservoir to the bottom of the secondary reservoir through the opening, wherein the sample is introduced from the inlet reservoir into the secondary reservoir through the connection channel and the opening of the bottom of the secondary reservoir, an area of the bottom being larger than the cross-section area of the connection channel;', 'a flat member which comprises a liquid-permeable porous material and placed on the bottom of the secondary reservoir so as to cover the opening of the secondary reservoir, contacts with the sample reaching to the secondary reservoir, and allows the sample in the connection channel to enter the secondary reservoir; and', 'a capillary tube in communication with the connection channel at a location between the inlet reservoir and the secondary reservoir, wherein a voltage is applied to the capillary tube for causing electrophoresis.'], '#text': 'an inlet reservoir into which a sample is introduced;'}16. The analytical tool according to claim 15 , wherein the flat member covers at least a part of the bottom of the secondary reservoir and allowing gas flow.17. The analytical tool according to claim ...

ISOLATION OF CELLS OF EPITHELIAL ORIGIN CIRCULATING IN PERIPHERAL BLOOD

The present invention provides a method for identifying epithelial cells circulating in peripheral blood, which allows individuals who suffer from a disease that presents with epithelial cell destruction to be discriminated from those who do not. The invention also relates to a kit or device for carrying out the methods of the invention. 121.-. (canceled)22. A method for obtaining data useful for the diagnosis , prognosis and classification of an individual having a disease that presents with tissue destruction and release of lung epithelial cells , said method comprising:{'claim-text': 'where said marker comprises an intracytoplasmic marker, a nuclear marker, a surface marker or a combination thereof', '#text': '(a) incubating a peripheral blood sample with a marker for lung epithelial cells,'}(b) identifying the lung epithelial cells by means of immunocytochemical, molecular and/or cytogenetic techniques;(c) quantifying the number of isolated cells; and(d) classifying the individual in a group of individuals who have a risk of suffering from a disease that presents with tissue destruction and release of epithelial cells when the presence of epithelial cells is identified in peripheral blood.23. The method of further comprising prior to step (a) subjecting the sample of peripheral blood of the individual to a density gradient separation.24. The method of further comprising prior to step (a) one or more of the following steps: subjecting the sample of peripheral blood of the individual to a density gradient separation claim 22 , after the density gradient separation claim 22 , collecting an interphase and washing the interphase with saline buffer before incubating the sample with the markers.25. The method of claim 22 , wherein the intracytoplasmic marker comprises a Cytokeratin claim 22 , a Vimentin claim 22 , a SP1 (Surfactant A and B antigen) claim 22 , or a combination thereof;wherein the nuclear marker comprises a transcription factor; andwherein the surface ...

MICROFLUIDIC DEVICE, SYSTEM AND METHOD

A combination of components in a capillary flow channel use capillary forces to passively control the movement of liquid samples within a microfluidic device. To detect a target, a liquid sample introduced to a proximal portion of capillary channel of a microfluidic device moves by capillary action along the specific components of capillary channel. 1. A microfluidic device , comprising:a capillary flow channel comprising a proximal opening and a distal opening;a filter pocket comprising a filter and disposed substantially at the proximal opening;a mixing well, disposed distal to the filter pocket;a dry reagent zone containing a reagent, the dry reagent zone disposed distal to the mixing well;a detection zone, disposed distal to the drug reagent zone,wherein the distal opening is tapered to control a flow rate, and wherein the filter pocket, the mixing well, the dry reagent zone and the detection zone are in fluidic communication.2. The microfluidic device of claim 1 , wherein the filter pocket comprises a sample inlet having a recess configured to receive a liquid sample claim 1 , a filter landing claim 1 , and a vent configured to permit air to be displaced upon receiving the liquid sample.3. The microfluidic device of claim 2 , wherein the filter landing includes a raised plateau extending from a distal edge of the filter pocket.4. The microfluidic device of claim 2 , wherein the filter pocket further comprises a catwalk strip disposed for directing a fluid deposited at the sample inlet to the filter landing.5. The microfluidic device of claim 1 , wherein the mixing well is dimensioned to move a filtered liquid sample by capillary action.6. The microfluidic device of claim 1 , wherein the dry reagent zone contains walls with a hydrophobic ink.7. The microfluidic device of claim 1 , further comprising a pinch region disposed distal to the dry reagent zone.8. The microfluidic device of claim 7 , wherein the pinch region is configured with a lobe claim 7 , ...

CELL IMAGING METHOD, CELL IMAGING APPARATUS, PARTICLE IMAGING METHOD, AND PARTICLE IMAGING APPARATUS

Disclosed is a cell imaging method including: forming a light sheet with respect to a flow cell; causing a measurement sample containing a plurality of cells to flow in the flow cell; and receiving lights generated from the plurality of cells passing through the light sheet, by an imaging device via an element configured to extend a depth of focus, and taking images of the plurality of cells by the imaging device. 1. A cell imaging method comprising:forming a light sheet with respect to a flow cell;causing a measurement sample containing a plurality of cells to flow in the flow cell; andreceiving lights generated from the plurality of cells passing through the light sheet, by an imaging device via an element configured to extend a depth of focus, and taking images of the plurality of cells by the imaging device.2. The cell imaging method of claim 1 , wherein the light sheet is formed to be inclined such that the light sheet is not perpendicular to a flow direction of the sample.3. The cell imaging method of claim 1 , wherein the plurality of cells are caused to simultaneously pass through the light sheet claim 1 , and lights generated from the plurality of cells are received by the imaging device.4. The cell imaging method of claim 1 , wherein the element configured to extend the depth of focus is a phase modulation element configured to modulate a point spread function.5. The cell imaging method of claim 4 , wherein the point spread function is a spiral point spread function.6. The cell imaging method of claim 4 , wherein the point spread function is a single-helix point spread function.7. The cell imaging method of claim 4 , further comprising subjecting an image including taken images of the plurality of cells to correction of distortion of each taken image claim 4 , the distortion being caused by the phase modulation element.8. The cell imaging method of claim 7 , wherein in the correcting of the distortion of the taken image claim 7 , image elements forming the ...

DYNAMIC RANGE EXTENSION SYSTEMS AND METHODS FOR PARTICLE ANALYSIS IN BLOOD SAMPLES

For analyzing a sample containing particles of at least two categories, such as a sample containing blood cells, a particle counter subject to a detection limit is coupled with an analyzer capable of discerning particle number ratios, such as a visual analyzer, and a processor. A first category of particles can be present beyond detection range limits while a second category of particles is present within respective detection range limits. The concentration of the second category of particles is determined by the particle counter. A ratio of counts of the first category to the second category is determined on the analyzer. The concentration of particles in the first category is calculated on the processor based on the ratio and the count or concentration of particles in the second category. 1. A method for measuring a quantity of a first cell type in a blood fluid sample , the sample including a second cell type , the method comprising:determining a population of the second cell type in a first volume of the sample by flowing the first volume through a hematology cell counter;acquiring images of a first number of the first type cells and a second number of the second cell types by injecting a second volume of the sample into a sheath fluid flowing within a flowcell so as to provide a sample stream having a thickness and a width greater than the thickness, the acquired images being acquired along an image path traversing the thickness of the sample stream;determining a ratio of the first number of the first cell type to the second number of the second cell types using the acquired images; andcalculating a cell quantity measure of the first cell type in the sample using the ratio and the population of the second cell type.2. The method of claim 1 , wherein the cell quantity measure comprises a cell concentration for the first cell type in the blood fluid sample.3. The method of claim 1 , wherein the cell quantity measure comprises a cell count for the first cell type ...

Bioparticle measuring method

Disclosed is a bioparticle measuring method including forming on a solid phase a complex of a sample containing a bioparticle sampled from a specimen, a capturer containing a tag which binds to the solid phase and capable of binding to the bioparticle, and a detector capable of binding to the bioparticle and containing an labeled substance; dissociating a part or a whole of the complex from the solid phase to prepare a measurement sample containing a part or a whole of the complex not fixed on the solid phase; and detecting signals from the measurement sample by a particle analyzer.

DEVICE AND METHOD FOR DRIED FLUID SPOT ANALYSIS

A lab-on-chip-based system is described for the direct and multiple sampling, control of the volume, fluid filtration, biochemical reactions, sample transfer, and dried spot generation on the conventional and commercial cards for dried fluid spot. Within an all-in-one integrated holder, the invention allows the complete process required to ensure a quantitative analysis of blood, plasma or any other fluids, modification and enrichment of molecule subsets, and formation of a dried fluid spot on the specific spot location of a passive cellulose, non-cellulose, absorbent, or non-absorbent membrane material sampling. 2. The device according to wherein the said card holding element comprises several fluid channels.3. The device according to comprising a lid which is adapted to be fixed to the spacer; said lid claim 1 , when fixed to said spacer being furthermore designed to bring the card into contact with the outlet.4. The device according to claim 1 , wherein each inlet is provided with a filter.5. The device according to claim 1 , wherein the channel is biochemically functionalized.6. The device according to comprising several channels which are biochemically functionalized in a different manner from each other.7. The device according to claim 1 , wherein said card flexible.8. A method for using a device as defined in and comprising the following successive steps:fixing a card on said spacer(s),bringing a predefined volume of a fluid to collect into contact with said inlet,waiting until said fluid has reached said outlet,forcing said card onto said outlet in such a way as to obtain a dried fluid spot on said card.9. The method according to wherein said card is forced onto said outlet with a lid.10. The method according to wherein said channels are fractionated to serially combine different sample/molecule enrichments claim 8 , separations and transformations.11. The device according to claim 3 , wherein the lid is fixed to the spacer by clipping. The present invention ...

Morphinan Compounds

This disclosure relates to novel morphinan compounds and their derivatives, pharmaceutically acceptable salts, solvates, and hydrates thereof. This disclosure also provides compositions comprising a compound of this disclosure and the use of such compositions in methods of treating diseases and conditions that are beneficially treated by administering a σ 1 receptor agonist that also has NMDA antagonist activity.

SAMPLE INJECTION DEVICE FOR FLOW-TYPE ANALYSIS DEVICE, FLOW-TYPE ANALYSIS DEVICE, AND MEASUREMENT METHOD FOR HEMOGLOBIN COMPONENT

Provided is a sample injection device for flow-type analysis including a cylindrical needle () which penetrates through an upper wall and a lower wall of a sample injection portion () of a carrier-liquid channel through ring-like sealing members (). The needle () includes an inner hole () which is closed on a side of a lower end of the needle () and open on an outer peripheral surface as a horizontal hole (). The needle moving unit () induces the needle () to move downward so that the horizontal hole () faces an inside of a sample vessel () to draw the sample to the inside of the needle (). Then the moving unit () induces the needle () to move upward so that the horizontal hole () faces an inside of the sample injection portion () to inject the sample in the inside of the needle (). At an intermediate position, washing liquid is discharged from the horizontal hole () of the needle (), and the washing liquid is recovered via a discharge path (). 1. A sample injection device for flow-type analysis configured to inject a sample into a carrier-liquid channel , the device comprising:a cylindrical needle which extends in a vertical direction and penetrates through an upper wall and a lower wall of the carrier-liquid channel at a sample injection position;ring-like sealing members that seal the penetrated portions;a vessel holding portion arranged below the lower wall and which holds a vessel in which a sample is contained;a needle moving unit capable of causing the needle to move in a vertical direction; anda measurement pump which can be connected with the needle on an upper end of the needle,wherein the cylindrical needle includes an inner hole which is closed on a side of the lower end thereof and open on an outer peripheral surface as a horizontal hole,wherein the needle moving unit is capable of causing the needle to move in the vertical direction to at least a sample drawing position at which the horizontal hole is positioned below the lower wall and faces an inside ...

Hematology systems and methods

Aspects and embodiments of the instant disclosure provide a particle and/or intracellular organelle alignment agent for a particle analyzer used to analyze particles contained in a sample. An exemplary particle and/or intracellular organelle alignment agent includes an aqueous solution, a viscosity modifier, and/or a buffer. Embodiments also encompass systems, compositions, and methods for analyzing a sample containing particles. Particles such as blood cells can be categorized and counted by a digital image processor. A digital microscope camera can be directed, for example using certain focusing techniques, into a flowcell defining a symmetrically narrowing flowpath in which the sample stream flows in a ribbon flattened by flow and viscosity parameters between layers of sheath fluid. Blood cell images can be collected and analyzed using dynamic range extension processes and systems.

MEASURING DEVICE, DIALYSIS END CONDITION DETERMINING DEVICE, AND DIALYSIS PROGRESS PRESENTING DEVICE

A measuring device () includes an analysis unit () that holds a fluid pool (B) of blood or serum therein, an irradiation unit () that irradiates fluorescent substances contained in the fluid pool (B) with excitation light, and a light receiving unit () that receives fluorescence generated from the fluorescent substances. An amount of the fluorescent substances contained in the blood or the serum after artificial dialysis is estimated from the intensity of the fluorescence. 1. A measuring device comprising:an analysis unit that holds blood or serum therein;an irradiation unit that irradiates fluorescent substances in the blood or the serum with excitation light; anda light receiving unit that receives fluorescence generated upon irradiation with the excitation light.2. The measuring device according to claim 1 , wherein the analysis unit includes an inflow port through which the blood or the serum flows in claim 1 , and has a capacity adapted to form a fluid pool of the blood or the serum when the blood or the serum is held in the analysis unit.3. The measuring device according to claim 2 , wherein the analysis unit includes a hole through which a syringe is inserted to sample the blood or the serum from the fluid pool.4. The measuring device according to claim 1 , further comprising a mechanism that obtains the serum through centrifugal separation of the blood.5. The measuring device according to claim 1 , wherein when the blood or the serum branched from a flow passage claim 1 , through which the blood or the serum flows at a first flow rate claim 1 , flows into the analysis unit claim 1 , the measuring device further comprises a regulator that decelerates the blood or the serum flowing into the analysis unit to a second flow rate.6. The measuring device according to claim 5 , wherein the second flow rate is 10 ml/min or less.7. The measuring device according to claim 1 , wherein the analysis unit is a drip chamber.8. The measuring device according to claim 1 , ...

MICROFLUIDIC DEVICE FOR SERIAL FLUIDIC OPERATIONS

An integrated microfluidic device for carrying out a series of fluidic operations includes a housing including a plurality of n microfluidic conduits, wherein n is at least three, and a rotating valve having an internal channel with an entrance port and an exit port that are angularly separated. The rotating valve is positionable in a first position to connect two of the n fluidic conduits via the internal channel, and upon rotating the valve to a second position, two other of the n fluidic conduits are connected by the internal channel. The device further may include one or more fluidic chambers in fluid communication with respective fluidic conduits. Fluid contained in one fluidic chamber is transferrable by application of positive or negative gas pressure through associated fluidic conduits into another fluidic chamber via the internal channel. The device may be utilized to perform a variety of fluidic operations. 1. An integrated microfluidic device for carrying out a series of fluidic operations comprising:a housing including a plurality of n microfluidic conduits, wherein n is at least three; anda rotating valve having an internal channel with an entrance port and an exit port that are angularly separated;wherein the rotating valve is positionable in a first position to connect two of the n fluidic conduits via the internal channel, and upon rotating the valve to a second position, two of the n fluidic conduits, including at least one fluidic conduit different from the fluidic conduits connected in the first position, are connected by the internal channel; andwherein in either the first position or the second position, two sequentially adjacent fluidic conduits are connected via the internal channel.2. The integrated microfluidic device of claim 1 , wherein the two of the n fluidic conduits connected in the second position includes at least one of the fluidic conduits connected to the internal channel in the first position.3. The integrated microfluidic device ...

FILTER FOR FILTERING NUCLEATED CELLS AND FILTERING METHOD USING THE SAME

A filter for filtering nucleated cells that includes a body containing at least either a metal or a metal oxide as its main component; and plural through holes, each of which have a shape other than a square shape, formed therein. An arithmetic average roughness of a first side of the filter part is smaller than a size of a nucleus of the nucleated cells to be filtered. 1. A filter for filtering nucleated cells , the filter comprising:a body having a first principal surface and a second principal surface that face each other, the body containing at least a metal as a main component thereof or a metal oxide as a main component thereof, and the body defining a plurality of through holes penetrating from the first principal surface to the second principal surface, each of the plurality of through holes having a shape other than a square shape,wherein the body comprises a filter part and a frame part, and the frame part is arranged to surround an outer circumference of the filter part,wherein the first principal surface consists of a first side of the frame part, a second side of the frame part, and a first side of the filter part, and the first side of the filter part is located immediately between the first side of the frame part and the second side of the frame part,wherein the second principal surface consists of a third side of the frame part, a fourth side of the frame part, and a second side of the filter part, and the second side of the filter part is located immediately between the third side of the frame part and the fourth side of the frame part,wherein the first side of the frame part and the third side of the frame part face each other,wherein the second side of the frame part and the fourth side of the frame part face each other,wherein the first side of the filter part and the second side of the filter part face each other,wherein the entire first side of the filter part is in the shape of a concave face such that the filter part has a film thickness that ...

Multiple laminar flow-based particle and cellular separation with laser steering

The invention provides a method, apparatus and system for separating blood and other types of cellular components, and can be combined with holographic optical trapping manipulation or other forms of optical tweezing. One of the exemplary methods includes providing a first flow having a plurality of blood components; providing a second flow; contacting the first flow with the second flow to provide a first separation region; and differentially sedimenting a first blood cellular component of the plurality of blood components into the second flow while concurrently maintaining a second blood cellular component of the plurality of blood components in the first flow. The second flow having the first blood cellular component is then differentially removed from the first flow having the second blood cellular component. Holographic optical traps may also be utilized in conjunction with the various flows to move selected components from one flow to another, as part of or in addition to a separation stage.

ANALYZING DEVICE

A solution component separated in a separating cavity is connected to a measuring cavity through a connecting channel and a measurement channel , and a first capillary cavity is provided on one side of the separating cavity so as to communicate with the connecting channel . The first capillary cavity is formed to extend to the outside of a separation interface of a sample liquid separated in the separating cavity 118-. (canceled)19. An analyzing device comprising:a rotation axis;a separating cavity having first, second and third sidewalls, the first sidewall being formed along the outermost periphery of the separating cavity around the rotation axis and connecting the second and the third sidewalls, the second sidewall extending from a connecting position between the first and the second sidewalls toward the rotation axis, and the third sidewall extending from a connecting position between the first and third sidewalls toward the rotation axis;a measurement channel configured to allow a sample liquid to be filled therein by a capillary force and having a capacity of a first predetermined amount;a first connecting channel configured to allow the sample liquid to be filled therein by a capillary force, and having a first end connected to the second sidewall of the separating cavity and a second end connected to the measurement channel, the second end of the first connecting channel located closer to the rotation axis than the first end of the first connecting channel, the first end of the first connecting channel located closer to the rotation axis than the connecting position between the first and second sidewalls of the separating cavity, the measurement channel including a space extending from the second end of the first connecting channel to a direction getting far from the rotation axis;a second connecting channel configured to allow the sample liquid to be filled therein by a capillary force; anda first capillary cavity configured to allow the sample liquid to ...

Methods for Segregating Particles Using an Apparatus with a Size-Discriminating Separation Element Having an Elongate Leading Edge

The disclosure relates to an apparatus for segregating particles on the basis of their ability to flow through a stepped passageway. At least some of the particles are unable to pass through a narrower passageway bounded by a segregating step, resulting in segregation of the particles. The breadth of the leading edge of at least one step of the apparatus is significantly greater than the overall width of the passageway in which the step occurs, permitting high and rapid sample throughput. The apparatus and methods described herein can be used to segregate particles of a wide variety of types. By way of example, they can be used to segregate circulating tumor cells from a human blood sample. 132-. (canceled)33. A method of segregating circulating endothelial cells (CECs) from blood cells in a fluid sample , wherein said method employs a device comprising:a body and a separation element that segregates an inlet region and an outlet region of the void, the separation element defining, together with a surface of the void,', 'a channel that fluidly connects the inlet and outlet regions by way of a separating portion into which the separation element projects, the channel having', 'an overall width at the separating portion and', 'a height defined by the distance between the separation element and the surface of the void,, 'a cover that define a void therebetween, the void containing'}at least one of the body, the cover or the separation element bearing a segregating step disposed within and having a leading edge extending substantially completely across the separating portion of the channel, whereby the channel is divided into an upstream portion on the inlet side of the leading edge and a substantially lamellar downstream portion on the outlet side of the leading edge, the upstream portion of the channel being also lamellar in a region between the inlet region and the separation element;the height of the upstream portion being sufficient to facilitate passage ...

CASCADED DESIGN MICROFLUIDIC STRUCTURE WITH STEP DIFFERENCE

The present invention provides a microfluidic structure for sorting targeted substances. The microfluidic structure includes: an inlet portion having at least one fluid input port; an outlet portion having a plurality of fluid output ports; a first annular flow channel communicated with the inlet portion at the upstream end and rotatably extended; and a second annular flow channel communicated with the downstream end of the first annular flow channel at the upstream end, communicated with the outlet portion at the downstream end and rotatably extended. The first annular flow channel and the second annular flow channel are connected in series according to an S-shaped track, so that the outer side wall of the first annular flow channel is continuously connected to the inner side wall of the second annular flow channel. The cross-sections of the first annular flow channel and the second annular flow channel have a height difference.

Biological Fluid Collection Device and Biological Fluid Separation and Testing System

A biological fluid collection device that is adapted to receive a blood sample having a cellular portion and a plasma portion is disclosed. After collecting the blood sample, the biological fluid collection device is able to transfer the blood sample to a point-of-care testing device or a biological fluid separation and testing device. After transferring the blood sample, the biological fluid separation and testing device is able to separate the plasma portion from the cellular portion and analyze the blood sample and obtain test results. 1. A biological fluid separation device , comprising:a rotatable body having a center of rotation, an outer periphery, and a body inlet, the body inlet adapted to receive a multi-component blood sample;a separation chamber defined within the rotatable body and in fluid communication with the body inlet and having a chamber outlet spaced apart from the body inlet, the separation chamber adapted to receive the multi-component blood sample; anda blood component chamber defined within the rotatable body and in fluid communication with the chamber outlet,wherein when the separation chamber contains the multi-component blood sample and a rotational force is applied to the rotatable body, a first blood component of the multi-component blood sample passes from the separation chamber into the blood component chamber and a second blood component of the multi-component blood sample is retained within the separation chamber,wherein the blood component chamber is disposed adjacent the center of rotation and the separation chamber is disposed adjacent the outer periphery of the rotatable body, andwherein the body inlet includes an engagement portion configured to receive a transfer device for closed transfer of a multi-component blood sample to the separation device.2. The biological fluid separation device of claim 1 , wherein the first blood component is a plasma component of the multi-component blood sample and the second blood component is a ...

BLOOD FILTER AND METHOD FOR FILTERING BLOOD

The present invention relates to a method for filtering blood to produce plasma or serum and to a blood filter for the production of plasma or serum from a blood sample. The invention further relates to a kit comprising a blood filter for the production of plasma or serum from a blood sample and a syringe. 1. A blood filter for the production of plasma or serum from a blood sample , comprising:a filter membrane having opposite first and second sides;a receiving compartment defining a hollow space for receiving a blood sample to be filtered, said receiving compartment being arranged on said first side of the filter membrane, the filter membrane resting on a seating, wherein at least 50% of the surface of the second side of the filter membrane is in contact with said seating, and wherein the seating comprises a plurality of channels for collecting the filtered blood sample.2. The blood filter of claim 1 , wherein the seating is convex having a radius of curvature between 30 and 150 mm.3. The blood filter of claim 2 , wherein at least 75% of the surface of the second side of the filter membrane is in contact with said seating.4. The blood filter of claim 3 , wherein the plurality of channels cover between 0.5% and 15% of the surface of the seating.5. The blood filter of claim 4 , wherein the plurality of channels forms a sampling compartment having a volume in the range between 0.06 mmand 3.5 mmper cmof the filter surface.6. The blood filter of claim 1 , wherein the blood filter comprises a single filter membrane. This application is a divisional of U.S. patent application Ser. No. 14/964,049, filed Dec. 9, 2015, which is a divisional of U.S. patent application Ser. No. 13/378,991, having a filing date of Jan. 30, 2012, which is a U.S. National Stage Application filed under 35 U.S.C. § 371 based upon International Application No. PCT/EP2010/058551, filed Jun. 17, 2010, which application claims priority to European Application No. 09163006.1, filed Jun. 17, 2009, the ...

SPECIMEN PROCESSING APPARATUS, SPECIMEN MEASUREMENT SYSTEM AND METHOD FOR PROCESSING SPECIMEN

A specimen processing apparatus performs processing on a specimen contained in a container. The specimen processing apparatus includes: holders having different shapes, each of the holders being configured to hold the container; a holder placement unit that comprises holder receiving portions having different shapes, the shapes of the holder receiving portions corresponding to the shapes of the holders; and a specimen processing unit that performs processing on the specimen contained in the container held by one of the holders placed on the holder placement unit. 1. A specimen processing apparatus that performs processing on a specimen contained in a container , comprising:holders having different shapes, each of the holders being configured to hold the container;a holder placement unit that comprises holder receiving portions having different shapes, the shapes of the holder receiving portions corresponding to the shapes of the holders; anda specimen processing unit that performs processing on the specimen contained in the container held by one of the holders placed on the holder placement unit.2. The specimen processing apparatus according to claim 1 , whereineach of the holder receiving portions has a shape that receives the respective holder only in a specific orientation.3. The specimen processing apparatus according to claim 1 , further comprisinga holder detector that detects the holder being placed on the holder placement unit.4. The specimen processing apparatus according to claim 3 , further comprisinga controller that, in response to the holder detection unit detecting that the holder is placed on the holder placement unit, causes the specimen processing unit to perform processing on the specimen contained in the detected holder.5. The specimen processing apparatus according to claim 3 , further comprisinga start-instruction reception unit that receives an instruction to start processing on the specimen, whereinthe controller prohibits the specimen ...

Biological Fluid Collection Device and Biological Fluid Separation and Testing System

A biological fluid collection device that is adapted to receive a blood sample having a cellular portion and a plasma portion is disclosed. After collecting the blood sample, the biological fluid collection device is able to transfer the blood sample to a point-of-care testing device or a biological fluid separation and testing device. After transferring the blood sample, the biological fluid separation and testing device is able to separate the plasma portion from the cellular portion and analyze the blood sample and obtain test results. 1. A biological fluid separation device , comprising:a rotatable body having a center of rotation and an outer periphery, the rotatable body having a body inlet adapted to receive a multi-component blood sample;a separation chamber defined within the rotatable body and in fluid communication with the body inlet and having a chamber outlet spaced apart from the body inlet, the separation chamber adapted to receive both the multi-component blood sample; anda blood component chamber defined within the rotatable body and in fluid communication with the chamber outlet, wherein when the separation chamber contains the multi-component blood sample and a rotational force is applied to the rotatable body, a blood component of the multi-component blood sample passes from the separation chamber into the blood component chamber and a second component of the multi-component blood sample is retained within the separation chamber,wherein the blood component chamber is disposed adjacent the center of rotation and the separation chamber is disposed adjacent the outer periphery of the rotatable body.2. The biological fluid separation device of claim 1 , wherein the blood component is a plasma component of the multi-component blood sample and the second component is a cellular component of the multi-component blood sample.3. The biological fluid separation device of claim 1 , further comprising a diagnostic chamber in fluid communication with the ...

BIOLOGICAL FLUID SEPARATION DEVICE

A biological fluid separation device adapted to receive a biological fluid sample having a first portion and a second portion is disclosed. The device includes a housing having a first chamber having a first chamber inlet for receiving the biological fluid sample therein and a first chamber outlet. The housing has a second chamber having a second chamber inlet and a second chamber outlet, and a separation member separating at least a portion of the first chamber outlet and the second chamber. The separation member is adapted to restrain the first portion of the biological fluid sample within the first chamber and to allow at least a portion of the second portion of the biological fluid sample to pass into the second chamber. An actuator, such as a vacuum source, draws the biological fluid sample into the first chamber and the second portion into the second chamber. 123-. (canceled)24. A biological fluid separation device adapted to receive a biological fluid sample having a first portion and a second portion , the biological fluid separation device comprising:a housing, comprising:a first chamber having a first chamber inlet for receiving the biological fluid sample therein and a first chamber outlet wherein the outlet is placed a lateral distance from the inlet in the chamber,a second chamber having a second chamber inlet and a second chamber outlet, and a separation member separating at least a portion of the first chamber outlet and the second chamber, wherein the separation member defines a tangential flow path along the lateral distance from the first chamber inlet to the first chamber outlet and is adapted to restrain the first portion of the biological fluid sample within the first chamber and to allow at least a portion of the second portion of the biological fluid sample to pass into the second chamber; andan actuator in communication with a portion of the housing, wherein the actuator is a vacuum source that provides a first pressure at the first chamber ...

Bodily fluid sample collection and transport

Bodily fluid sample collection systems, devices, and method are provided. The sample is collected at a first location and subjected to a first sample processing step. The sample may be shipped to a second location and subjected to a second sample processing step that does not introduce contaminants into a plasma portion of the sample formed from the first processing step. The sample may also be mixed with other material(s) in the collection device.

Biological Fluid Separation Device and Biological Fluid Separation and Testing System

A biological fluid separation device that is adapted to receive a multi-component blood sample is disclosed. After collecting the blood sample, the biological fluid separation device is able to separate a plasma portion from a cellular portion. After separation, the biological fluid separation device is able to transfer the plasma portion of the blood sample to a point-of-care testing device. The biological fluid separation device of the present disclosure also provides a closed separation and transfer system that reduces the exposure of a blood sample and provides fast mixing of a blood sample with a sample stabilizer. The biological fluid separation device is engageable with a blood testing device for closed transfer of a portion of the plasma portion from the biological fluid separation device to the blood testing device. The blood testing device is adapted to receive the plasma portion to analyze the blood sample and obtain test results. 1. A biological fluid separation device adapted to receive a multi-component blood sample , the biological fluid separation device comprising:a separation cartridge having an inlet port and a flow channel defined within the cartridge in fluid communication with the inlet port, the flow channel containing a separation element adapted to separate the multi-component blood sample into at least a first component and a second component, a first collection chamber defined within the cartridge in fluid communication with the flow channel and including a first outlet port, and a second collection chamber defined within the cartridge in fluid communication with the flow channel and including a second outlet port, the second collection chamber isolated from the first collection chamber.2. The biological fluid separation device of claim 1 , wherein the first component is a cellular portion of the multi-component blood sample and the second component is a plasma portion of the multi-component blood sample.3. The biological fluid separation ...

Biological Fluid Separation Device and Biological Fluid Separation and Testing System

A biological fluid separation device that is adapted to receive a multi-component blood sample is disclosed. After collecting the blood sample, the biological fluid separation device is able to separate a plasma portion from a cellular portion. After separation, the biological fluid separation device is able to transfer the plasma portion of the blood sample to a point-of-care testing device. The biological fluid separation device of the present disclosure also provides a closed separation and transfer system that reduces the exposure of a blood sample and provides fast mixing of a blood sample with a sample stabilizer. The biological fluid separation device is engageable with a blood testing device for closed transfer of a portion of the plasma portion from the biological fluid separation device to the blood testing device. The blood testing device is adapted to receive the plasma portion to analyze the blood sample and obtain test results. 1. A biological fluid separation cartridge , comprising:a housing having an inlet port and a flow channel defined within the housing in fluid communication with the inlet port;a first collection chamber defined within the housing in fluid communication with the flow channel and including a first outlet port having a valve comprising a deformable wall member and a septum, wherein the septum covers the first outlet port; anda second collection chamber defined within the housing in fluid communication with the flow channel and including a second outlet port, the second collection chamber isolated from the first collection chamber, and the second outlet port spaced apart from the first outlet port.2. The biological fluid separation cartridge of claim 1 , wherein the flow channel has a spiral shape.3. The biological fluid separation cartridge of claim 1 , wherein at least a portion of the flow channel contains a sample stabilizer.4. The biological fluid separation cartridge of claim 1 , further comprising an inlet channel in fluid ...

MULTILAYER DEVICE FOR SEPARATING BLOOD COMPONENTS AND USES THEREOF

Embodiments of the invention are generally directed to analyte detection and products facilitating the collection, separation of sample components, and analyte detection. The multilayer device that allows for a rapid, easy, accurate, and efficient test of a fluid sample for analytes of interest and methods of collecting, separating components, and testing using the multilayer device are described in various embodiments of the invention. 1) A multilayer device , comprising:a) a top unit, wherein said top unit comprises a filtration membrane unit adjacent to a hydrophobic membrane; andb) a bottom unit, wherein said bottom unit comprises a collection material and a bottom cover,wherein said top unit is adjacent to and connected to said bottom unit, said filtration membrane unit comprising at least one filtration membrane, said filtration membrane unit has a top surface and a bottom surface, and said hydrophobic membrane has a top surface and a bottom surface, where said bottom surface of said filtration membrane unit is adjacent to said top surface of said hydrophobic membrane, where said collection material has a top surface and a bottom surface, said bottom surface of said hydrophobic membrane is adjacent to said top surface of said collection material, and said bottom surface of said collection material is adjacent to said bottom cover.2) A multilayer device , comprising:a) a top unit comprising layers of: a top cover with at least one cutout, a filtration membrane unit, and a hydrophobic membrane with at least one cutout; andb) a bottom unit comprising layers of: a collection material and a bottom cover without cutouts,wherein said top unit is adjacent to and connected to said bottom unit, said filtration membrane unit comprises two filtration membranes of decreasing pore sizes with each having a shape of said cutout, said filtration membrane unit is positioned within said cutout of said top cover and adjacent to said hydrophobic membrane, said hydrophobic membrane ...

Method for detecting or separating/obtaining circulating tumor cell employing cell proliferation method

A method for detecting or separating/obtaining CTC, which is capable of reliably and stably detecting or separating/obtaining a circulating tumor cell and a circulating tumor stem cell present in trace amounts in a biological circulating fluid such as blood or lymph, even in the state where the cancer type of the tumor cells cannot be determined yet and the state where the tumor cells are present in trace amounts in the biological circulating fluid. The method is attained by detecting or separating/obtaining CTC and/or CTSC in a biological circulating fluid, comprising treatment steps (1) to (4): (1) pretreating a sample from the biological circulating fluid to obtain a mononuclear cell phase; (2) providing a well plate in which a culture medium consisting of a serum-free cell growth medium for circulating tumor cell and/or circulating tumor stem cell has been injected, and seeding thereto the mononuclear cells obtained in step (1), followed by incubation; (3) removing the culture medium from a well of the plate obtained by the incubation in step (2); and (4) detecting or separating/obtaining an adherent tumor cell attached to the well of the plate after step (3).

In-vitro diagnostic apparatus and in-vitro diagnostic test method

An in-vitro diagnostic apparatus includes a controller configured to set a test result reference range corresponding to a condition of a testee, among test result reference ranges corresponding to a plurality of conditions, with respect to a test item of a test object, and generate a user interface screen including the set test result reference range; and a display configured to display the user interface screen.

Microfluidic Chip for Acoustic Separation of Biological Objects

A microfluidic device for sorting biological objects includes a microfluidic chip including a planar substrate having first and second planar surfaces, the planar substrate including first and second networks of channels recessed respectively from the first and second planar surfaces and fluidically connected by way of at least a through-hole in the planar substrate; a first lid attached to the first planar surface of the planar substrate and substantially covering the first network of channels; and a second lid attached to the second planar surface of the planar substrate and substantially covering the second network of channels; and one or more piezoelectric transducers attached to the first lid and/or the second lid and configured to generate first and second acoustic standing waves in a first linear channel of the first network of channels and a second linear channel of the second network of channels, respectively. 1. A microfluidic device for sorting biological objects comprising:{'claim-text': ['a planar substrate having first and second planar surfaces, the planar substrate including first and second networks of channels recessed respectively from the first and second planar surfaces and fluidically connected in series or parallel by way of at least a through-hole in the planar substrate;', 'a first lid attached to the first planar surface of the planar substrate and substantially covering the first network of channels; and', 'a second lid attached to the second planar surface of the planar substrate and substantially covering the second network of channels; and'], '#text': 'a microfluidic chip including:'}one or more first piezoelectric transducers attached to an exterior of the first lid and configured to generate a first acoustic standing wave having a first wavelength in a first linear channel of the first network of channels.2. The microfluidic device of further comprising one or more second piezoelectric transducers attached to an exterior of the second ...