30-06-2023 дата публикации
Номер: CN116359408A
Принадлежит:
The invention discloses a pretreatment method for analyzing biogenic amine in cheese. The method comprises the following steps: adding a cheese sample to be detected into a formic acid aqueous solution, carrying out vortex mixing, carrying out heat-preservation water bath, adding acetonitrile, carrying out uniform mixing, carrying out ultrasonic extraction, centrifuging, and filtering with filter paper. Adding pure water into the filtrate, uniformly mixing, adding an enrichment material, uniformly mixing, and standing at room temperature; filtering with a sieve plate, adding an eluent into the enrichment material, placing at room temperature for 10-50 minutes, and eluting; after the eluent is filtered by a filter membrane, detecting the peak area of the biogenic amine by a high performance liquid chromatography-triple quadrupole tandem mass spectrometer, and quantifying by an external standard method to realize the analysis and determination of the biogenic amine in the cheese sample. The ...
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