Настройки

Укажите год
-

Небесная энциклопедия

Космические корабли и станции, автоматические КА и методы их проектирования, бортовые комплексы управления, системы и средства жизнеобеспечения, особенности технологии производства ракетно-космических систем

Подробнее
-

Мониторинг СМИ

Мониторинг СМИ и социальных сетей. Сканирование интернета, новостных сайтов, специализированных контентных площадок на базе мессенджеров. Гибкие настройки фильтров и первоначальных источников.

Подробнее

Форма поиска

Поддерживает ввод нескольких поисковых фраз (по одной на строку). При поиске обеспечивает поддержку морфологии русского и английского языка
Ведите корректный номера.
Ведите корректный номера.
Ведите корректный номера.
Ведите корректный номера.
Укажите год
Укажите год
Применить Всего найдено 21. Отображено 21.
23-05-2016 дата публикации

METHOD FOR PRODUCING COLLAGENASE AND METHOD FOR PRODUCING COLLAGEN TRIPEPTIDE BY USING SAME

Номер: KR1020160057294A
Автор: SHIN, YONG CHUL, PIAO ZHE, CHOI, SU LIM, WANG, EUN SEON, KIM, YI SOO, LIM, JAE MYUNG, JUNG, JIN HEE, WON, JU EUN, YOON, JI HOUN, KIM, TAE YOUNG
Принадлежит:

A method for producing collagenase having a high collagen tripeptide acquisition rate comprises: a first step of centrifuging a bacillus subtilis strain; a second step of concentrating a centrifuged supernatant; and a third step of refining the same by using ion exchange chromatography. A method for producing a collagen tripeptide comprises: a first step of mixing pretreated fish scale with water at a weight ratio of 2:8; a second sstep of heating the mixture at 90°C for 5 hours; a third step of feeding collagenase produced through the method for producing collagenase, and decomposing the collagenase at 35°C for 12 hours; a fourth step of removing foreign matieral through centrifuging a component obtained from the third step; a fifth step of refining the component by means of ion exchange chromatography; a sixth step of concentrating the refined component; a seventh step of refining the concentrated component by using activated carbon; and an eighth step of sterilizing the component by ...

Подробнее
Номер записи: 1
26-07-2016 дата публикации

MUTANT ALPHA-AMINO ESTER HYDROLASE WITH INCREASED AMOXICILLIN PRODUCTIVITY

Номер: KR1020160088757A
Автор: SHIN, YONG CHUL, PIAO ZHE, CHOI, SU LIM, WANG, EUN SEON, KANG, MI SUK, LEE, MI KYOUNG, KIM, SEUNG RAK, BAEK, JI YUN
Принадлежит:

The present invention relates to mutant alpha-amino ester hydrolase (α-amino acid ester hydrolase) with increased amoxicillin productivity and uses thereof. According to the present invention, in the case of mutant AEH, the synthetic yield of amoxicillin (Amox) is high, while the decomposition rate of HPGM is significantly low. Therefore, the productivity of amoxicillin is significantly increased, so the industrial applicability thereof is high. COPYRIGHT KIPO 2016 ...

Подробнее
Номер записи: 2
11-01-2018 дата публикации

MUTATED IMMUNOGLOBULIN-BINDING PROTEIN HAVING INCREASED ALKALINE TOLERANCE

Номер: WO2018009006A1
Автор: SHIN, Yong Chul, PIAO, Zhe, CHOI, Su-Lim, JO, Yang Ho, PARK, So-Young, KWON, Dae Beom
Принадлежит:

The present invention relates to a mutated immunoglobulin-binding protein having increased alkaline tolerance and, more specifically, to an immunoglobulin-binding protein in which, with respect to the A-domain of Staphylococcal protein A, or a functional variant thereof, an amino acid at a specific site is mutated and thereby exhibits an increased chemical stability at an alkaline pH value in comparison to a parental molecule. The present invention can provide an antibody-purifying immunoglobulin-binding protein ligand and matrix which have enhanced alkaline tolerance and accordingly enhanced stability in multiple times of alkaline cleaning.

Подробнее
Номер записи: 3
21-07-2016 дата публикации

MODIFIED Α -AMINO ACID ESTER HYDROLASE WITH INCREASED AMOXICILLIN PRODUCTIVITY

Номер: WO2016114479A1
Автор: SHIN, Yong Chul, PIAO, Zhe, CHOI, Su-Lim, WANG, Eun Seon, KANG, Mi Suk, LEE, Mi-Kyoung, KIM, Seung Rak, BAEK, Ji Yun
Принадлежит:

The present invention relates to a modified α-amino acid ester hydrolase with increased amoxicillin productivity, and a use thereof. The modified AEH of the present invention is highly industrially applicable since the modified AEH has a high synthetic yield of amoxicillin(Amox) as well as a remarkably low decomposition rate of HPGM, thereby significantly increasing productivity of amoxicillin.

Подробнее
Номер записи: 4
22-02-2018 дата публикации

콜라겐 분해효소 제조방법 및 이를 이용한 콜라겐 트리펩타이드의 제조방법

Номер: KR0101831431B1
Автор: 신용철, 박철, 최수림, 왕은선, 김이수, 임재명, 정진희, 원주은, 윤지훈, 김태영
Принадлежит: 아미코젠주식회사

... 본 발명인 콜라겐 분해효소의 제조방법은, 바실러스 서브틸리스 균주를 원심분리하는 제 1단계와, 원심분리된 상등액을 농축시키는 제 2단계와, 이온교환 크로마토그래피법을 이용하여 정제하는 제 3단계를 포함하여 구성되는 것을 특징으로 하며, 콜라겐 트리펩타이드 제조방법은, 전처리된 어린을 물과 2:8의 중량비로 혼합하는 제 1단계와, 상기 혼합물을 90℃에서 5시간 동안 열처리하는 제 2단계와, 상술한 방법에 의해 제조되는 콜라겐 분해효소를 투입하여 35℃에서 12시간동안 분해시키는 제 3단계와, 상기 제 3단계에서의 성분에서 원심분리를 통하여 이물질을 제거하는 제 4단계와, 상기 성분을 이온교환 크로마토그래피에 의해 정제하는 제 5단계와, 상기 정제된 성분을 농축시키는 제 6단계와, 상기 농축된 성분을 활성탄을 이용하여 정제하는 제 7단계와, 상기 성분을 필터를 이용하여 제균하는 제 8단계를 포함하여 구성되는 것을 특징으로 한다.

Подробнее
Номер записи: 5
16-08-2017 дата публикации

COLLAGEN HYDROLYSATE HAVING HIGH CONCENTRATION OF COLLAGEN TRIPEPTIDE AND USES THEREOF

Номер: KR1020170093694A
Автор: SHIN, YONG CHUL, PIAO ZHE, CHOI, SU LIM, KIM, YI SOO, KIM, HYUN A, CHO, YUN YOUNG, CHUNG, HYE JIN
Принадлежит:

The present invention relates to a collagen hydrolysate having high concentration of collagen tripeptide and uses thereof, and more specifically, to a collagen hydrolysate prepared using collagen hydrolase which specifically produces collagen tripeptide, and to a composition for promoting hair loss prevention and hair growth using the collagen hydrolysate as an effective ingredient. The collagen hydrolysate of the present invention has a high concentration of collagen tripeptide in comparison with previously reported collagen hydrolysates, and thus exhibits excellent absorption rate in the body, and has excellent hair loss prevention and hair growth promoting abilities, and skin wrinkle alleviation and skin moisturizing effects. COPYRIGHT KIPO 2017 ...

Подробнее
Номер записи: 6
16-12-2010 дата публикации

METHOD FOR SCREENING AND QUANTIFYING VARIOUS ENZYME ACTIVITIES USING A GENETIC ENZYME SCREENING SYSTEM

Номер: WO2010143871A3
Автор: LEE, Seung Goo, RHA, Eugene, CHOI, Su Lim, SONG, Jae Jun, CHOI, Jong Hyun, KIM, Hee Sik
Принадлежит:

The present invention relates to a method for sensing and quantifying a target enzyme activity and to a method for screening for a metagenomic library-derived target enzyme activity on the basis of the same. More particularly, the present invention relates to a method for producing a genetic enzyme screening system (GESS) for sensing phenol-based compounds and sensing and quantifying various enzyme activities using the system, and to a method for screening for a microactivity of the metagenome-derived target enzyme using the genetic enzyme screening system and ensuring a target enzyme gene. In addition, the method for screening and quantifying a target enzyme activity according to the present invention senses useful genes at an ultra high speed (million/day) at a single cell level, from an environment or various gene groups such as a metagenomic library, and quickly senses and quantifies various enzyme activities at a high sensitivity by using a sensitivity to phenol derivatives and expression ...

Подробнее
Номер записи: 7
16-01-2018 дата публикации

MUTATED IMMUNOGLOBULIN BINDING PROTEIN WITH ENHANCED ALKALI TOLERANCE

Номер: KR1020180005513A
Автор: SHIN, YONG CHUL, PIAO ZHE, CHOI, SU LIM, JO, YANG HO, PARK, SO YOUNG, KWON, DAE BEOM
Принадлежит:

The present invention relates to a mutated immunoglobulin binding protein with enhanced alkali tolerance and, more specifically, to an immunoglobulin binding protein showing increased chemical stability in alkali pH values when compared to parent molecules since amino acid is mutated in a specific position with respect to a domain A of a Staphylococcus protein A or functional mutants thereof. Provided are an immunoglobulin binding protein ligand for purifying antibodies with improved stability with respect to multiple alkali cleaning due to alkali tolerance, and matrix. COPYRIGHT KIPO 2018 ...

Подробнее
Номер записи: 8
08-09-2015 дата публикации

Method for Screening of Useful Enzymes from Metagenome Using Forced Transcription

Номер: KR0101550389B1
Автор: 이승구, 최수림, 나유진, 송재준, 이상준, 권길광, 김은미, 최종현, 권오석
Принадлежит: 한국생명공학연구원

... 본 발명은 메타게놈 라이브러리로부터 고효율로 유용효소를 탐색하는 방법에 관한 것으로, 더욱 자세하게는 메타게놈 유래 유전자를 양방향 프로모터를 채용한 강제전사 시스템을 이용하여 외래 유전자의 발현을 강제적으로 증가시키고, 전사조절단백질을 기반으로 하는 재설계 유전자회로와 함께 연계하여 사용하는 것을 특징으로 하는 고효율로 유용효소를 탐색하는 방법에 관한 것이다. 본 발명에 따르면, 기존에 발현효율이 낮아서 목적 유전자의 활성을 직접 탐색하는데 많은 어려움이 있었던 메타게놈 유래 외래 유전자를 양방향 프로모터를 채용한 강제전사 시스템을 이용하여 외래 유전자의 발현을 강제적으로 증가시키고, 전사조절단백질을 기반으로 하는 재설계 유전자회로와 함께 연계하여 사용함으로써, 목적 효소에 의하여 생성된 극미량의 반응산물 조차도 형광 및 항생제 저항성 등 리포터 단백질의 발현을 통하여 감지할 수 있어, 고효율로 목적 유전자의 선별 및 목적 유전자 활성의 정량측정이 가능하다.

Подробнее
Номер записи: 9
31-01-2019 дата публикации

MUTATED IMMUNOGLOBULIN-BINDING PROTEIN HAVING INCREASED ALKALINE TOLERANCE

Номер: WO2018009006A8
Автор: SHIN, Yong Chul, PIAO, Zhe, CHOI, Su-Lim, JO, Yang Ho, PARK, So-Young, KWON, Dae Beom
Принадлежит:

The present invention relates to a mutated immunoglobulin-binding protein having increased alkaline tolerance and, more specifically, to an immunoglobulin-binding protein in which, with respect to the A-domain of Staphylococcal protein A, or a functional variant thereof, an amino acid at a specific site is mutated and thereby exhibits an increased chemical stability at an alkaline pH value in comparison to a parental molecule. The present invention can provide an antibody-purifying immunoglobulin-binding protein ligand and matrix which have enhanced alkaline tolerance and accordingly enhanced stability in multiple times of alkaline cleaning.

Подробнее
Номер записи: 10
30-06-2011 дата публикации

METAGENOME-DERIVED ALKALINE PHOSPHATASE

Номер: KR2011078448A1
Автор: LEE, Seung Goo, CHOI, Su Lim, RHA, Eugene, SONG, Jae Jun
Принадлежит:

The present invention relates to metagenome-derived alkaline phosphatase, and more specifically to a novel alkaline phosphatase which is derived from metagenome screened using a re-engineered gene circuit which senses phenolic compounds. According to the present invention, the novel alkaline phosphatase has high DNA dephosphorylation activation and has a characteristic of quickly removing the activation by simple thermal treatment, and can thus be used in dephosphorylation for efficiency of gene manipulation including gene cloning. In addition, since active alkaline phosphatase can be expressed in a recombinant microorganism, the alkaline phosphatase can be used for the purpose of various analyses such as enzyme immune-assay and the like.

Подробнее
Номер записи: 11
10-08-2017 дата публикации

COLLAGEN HYDROLYSATE CONTAINING HIGH CONTENT OF COLLAGEN TRIPEPTIDE AND USE THEREOF

Номер: WO2017135754A1
Автор: SHIN, Yong Chul, PIAO, Zhe, CHOI, Su-Lim, KIM, Yi Soo, KIM, Hyun A, CHO, Yun Young, CHUNG, Hye Jin
Принадлежит:

The present invention relates to a collagen hydrolysate with a high content of collagen tripeptides, and the use thereof and, more particularly, to a collagen hydrolysate prepared by using a collagenase that specifically produces a predominant amount of collagen tripeptides and a composition comprising the same as an effective ingredient for preventing hair loss and/or for promoting hair regrowth. Remarkably rich in collagen tripeptide content compared to previously reported collagen hydrates, the collagen hydrolysate of the present invention has an outstanding absorption rate in the body and exhibits an excellent capability of preventing hair loss and promoting hair regrowth and an excellent effect of improving skin wrinkles and/or moisturizing the skin.

Подробнее
Номер записи: 12
16-12-2010 дата публикации

METHOD FOR SCREENING AND QUANTIFYING VARIOUS ENZYME ACTIVITIES USING A GENETIC ENZYME SCREENING SYSTEM

Номер: KR2010143871A9
Автор: LEE, Seung Goo, RHA, Eugene, CHOI, Su Lim, SONG, Jae Jun, CHOI, Jong Hyun, KIM, Hee Sik
Принадлежит:

The present invention relates to a method for sensing and quantifying a target enzyme activity and to a method for screening for a metagenomic library-derived target enzyme activity on the basis of the same. More particularly, the present invention relates to a method for producing a genetic enzyme screening system (GESS) for sensing phenol-based compounds and sensing and quantifying various enzyme activities using the system, and to a method for screening for a microactivity of the metagenome-derived target enzyme using the genetic enzyme screening system and ensuring a target enzyme gene. In addition, the method for screening and quantifying a target enzyme activity according to the present invention senses useful genes at an ultra high speed (million/day) at a single cell level, from an environment or various gene groups such as a metagenomic library, and quickly senses and quantifies various enzyme activities at a high sensitivity by using a sensitivity to phenol derivatives and expression ...

Подробнее
Номер записи: 13
04-10-2016 дата публикации

BOARD GAME SET FOR EDUCATION OF BIOLOGICAL RESOURCES

Номер: KR101661451B1
Автор: SONG, YEONG EUN, YU, JI HYUN, LEE, SEON HEE, CHOI, SU RIM, JO, GEUN HEE
Принадлежит: NATIONAL INSTITUTE OF BIOLOGICAL RESOURCES

According to the present invention, disclosed is a board game set for education of biological resources and a board game method for education of biological resources, which can provide biological resources education effect by each card while a die is cast by a board plate and multiple cards to move to a partitioned cell on the board plate, and enables children and adolescents to learn the biological resources without being easily tired and bored since they learn the biological resources through a board game. The board game set includes: the board plate comprising multiple partitioned cells along a surface circumference part so as to be able to move in accordance with the number from the cast die; and a token paid to a gamer when the game starts. The partitioned cell (10) comprises an orange biological resources technology card drawing cell (11) and a green event cell (12) provided sequentially from the left side of a start cell (20), and further comprises an event card drawing cell (13) ...

Подробнее
Номер записи: 14
19-05-2016 дата публикации

METHOD FOR PREPARING COLLAGENASE AND METHOD FOR PREPARING COLLAGEN TRIPEPTIDE USING SAME

Номер: WO2016076647A1
Автор: SHIN, Yongchul, PIAO, zhe, CHOI, Sulim, WANG, Eunseon, LIM, Jaemyung, KIM, Yisoo, JUNG, Jinhee, WON, Jueun, YOON, Jihoun, KIM, Taeyoung
Принадлежит:

A method for preparing collagenase of the present invention comprises: a first step for centrifuging a Bacillus subtilis strain; a second step for concentrating the centrifuged supernatant; and a third step for purifying the supernatant using ion exchange chromatography. A method for preparing a collagen tripeptide comprises: a first step for mixing pre-treated fish scales and water at a weight ratio of 2:8; a second step for heat-treating the mixture at 90°C for 5 hours; a third step for adding the collagenase prepared by the above-described method, followed by degradation at 35°C for 12 hours; a fourth step for removing foreign materials from the component in the third step through centrifugation; a fifth step for purifying the component by ion exchange chromatography; a sixth step for concentrating the purified component; a seventh step for purifying the concentrated component using activated carbon; and an eighth step for removing microorganisms from the component using a filter.

Подробнее
Номер записи: 15
13-07-2016 дата публикации

Method for Detecting and Quantitating Cellulase Using Artificial Genetic Circuit

Номер: KR0101639424B1
Автор: 이승구, 권길광, 나유진, 최수림, 김하성, 이대희
Принадлежит: 한국생명공학연구원

... 본 발명은 재설계 유전자 회로를 이용한 섬유소분해활성 효소의 신규 탐색방법에 관한 것으로, 더욱 자세하게는 재설계 유전자 회로를 이용한 섬유소분해활성 효소의 신규 탐색방법 및 상기 섬유소분해활성 효소에 의해 분해되는 셀로비오스의 정량방법에 관한 것이다.본 발명의 재설계 유전자회로를 이용하면, 셀로비오스의 양에 비례하는 셀로비오스 인지 전사조절 단백질(CelR)이 리포터 단백질의 발현을 유도하도록 함으로써, 셀로비오스를 고감도로 빠르게 감지하고 정량화 함으로서 셀로비오스의 감지 및 정량적 분석에 활용할 수 있고, 셀로비오스를 유리하는 exo형 섬유소분해효소의 활성을 측정하거나 새로운 exo형 섬유소분해효소 탐색할 수 있다.

Подробнее
Номер записи: 16
28-06-2016 дата публикации

Single Cell-based Screening Method of Useful Enzyme Resources Using Mesh-integrated Microdroplet Array

Номер: KR0101634350B1
Автор: 이승구, 박제균, 엄유진, 권길광, 나유진, 최수림
Принадлежит: 한국생명공학연구원, 한국과학기술원

... 본 발명은 단일세포의 활성을 분석하기 위해 분석하고자하는 세포를 단일 세포 단위로 함유할 수 있는 피코리터 수준의 미세액적 제조방법 및 메쉬-그리드가 도입된 미세 액적 마이크로웰 어레이를 이용하여 목적 유용 효소 활성의 탐색 방법에 관한 것이다. 본 발명에 따른 미세액적 제조 방법은 미세액적 제조 시 이차파쇄과정을 거치므로 단일세포 함유 미세액적의 생산효율을 증가시키고, 메쉬-그리드 기법을 이용한 미세액적(microdroplet) 마이크로 웰 어레이는 개방형 구조를 갖기 때문에 쉽게 액적을 포획하고 융합할 수 있으며 추가적인 조작과 다른 장치와의 연계할 수 있는 안정적 환경을 제공한다. 다양한 미생물 및 메타게놈 유래 대사 및 효소활성을 단일 세포 수준에서 고속 탐색하는 것을 가능하게 할 뿐만 아니라, 다양한 조건에서 미생물 간 상호작용을 연구하는 데에 적용될 수 있다.

Подробнее
Номер записи: 17
06-07-2015 дата публикации

NOVEL SEARCHING METHOD FOR MEASURING QUANTITATIVE CELLULASE USING GENETIC ENZYME SCREENING SYSTEM

Номер: KR1020150075967A
Автор: LEE, SEUNG GOO, KWON, KIL KOANG, RHA, EU GENE, CHOI, SU LIM, KIM, HA SEONG, LEE, DAE HEE
Принадлежит:

The present invention relates to a novel searching method of cellulase using a genetic enzyme screening system and, more specifically, to a novel searching method of cellulose using a genetic enzyme screening system, and to a method for measuring quantitative cellobiose decomposed by the cellulose. The genetic enzyme screening system enables a cellobiose transcription regulator in proportion to the amount of cellobiose to induce the expression of reporter proteins, thereby detecting and measuring quantitative cellobiose quickly and being used for detecting cellobiose and analyzing quantitative cellobiose while searching a novel exo-type or measuring activity of an exo-type cellulase which separates cellobiose. COPYRIGHT KIPO 2015 ...

Подробнее
Номер записи: 18
02-07-2015 дата публикации

NOVEL METHOD FOR SEARCHING AND QUANTIFYING CELLULASE USING REDESIGNED GENETIC CIRCUIT

Номер: WO2015099456A1
Автор: LEE, Seung Goo, KWON, Kil Koang, RHA, Eugene, CHOI, Su-Lim, KIM, Haseong, LEE, Dae-Hee
Принадлежит:

The present invention relates to a novel method for searching cellulase using a redesigned genetic circuit and, more specifically, to a novel method for searching cellulase using a redesigned genetic circuit and a method for quantifying cellobiose degraded by the cellulase. When the redesigned genetic circuit of the present invention is used, the cellobiose recognition transcription regulation protein (CelR), which is proportional to the amount of cellobiose, induces the expression of a reporter protein, thereby promptly sensing and quantifying cellobiose with high sensitivity, and thus the present invention can be utilized for cellobiose sensing and quantitative analysis, and enables the measurement of the activity of exo-type cellulase which frees cellobiose and the search of new exo-type cellulase.

Подробнее
Номер записи: 19
18-11-2016 дата публикации

아목시실린 생산성이 증가된 변이 알파-아미노산 에스터 가수분해효소

Номер: KR0101677755B1
Автор: 신용철, 박철, 최수림, 왕은선, 강미숙, 이미경, 김승락, 백지윤
Принадлежит: 아미코젠주식회사

... 본 발명은 아목시실린 생산성이 증가된 변이 알파-아미노산 에스터 가수분해효소(α-amino acid ester hydrolase)및 이의 이용에 관한 것이다. 본 발명의 변이 AEH는 아목시실린(Amox)의 합성수율은 높으면서도 HPGM의 분해율이 현저히 낮아, 아목시실린의 생산성이 현저하게 증가되었으므로 산업상 이용가능성이 높다.

Подробнее
Номер записи: 20
16-12-2010 дата публикации

METHOD FOR SCREENING AND QUANTIFYING VARIOUS ENZYME ACTIVITIES USING A GENETIC ENZYME SCREENING SYSTEM

Номер: KR2010143871A2
Автор: LEE, Seung Goo, RHA, Eugene, CHOI, Su Lim, SONG, Jae Jun, CHOI, Jong Hyun, KIM, Hee Sik
Принадлежит:

The present invention relates to a method for sensing and quantifying a target enzyme activity and to a method for screening for a metagenomic library-derived target enzyme activity on the basis of the same. More particularly, the present invention relates to a method for producing a genetic enzyme screening system (GESS) for sensing phenol-based compounds and sensing and quantifying various enzyme activities using the system, and to a method for screening for a microactivity of the metagenome-derived target enzyme using the genetic enzyme screening system and ensuring a target enzyme gene. In addition, the method for screening and quantifying a target enzyme activity according to the present invention senses useful genes at an ultra high speed (million/day) at a single cell level, from an environment or various gene groups such as a metagenomic library, and quickly senses and quantifies various enzyme activities at a high sensitivity by using a sensitivity to phenol derivatives and expression ...

Подробнее
Номер записи: 21