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Небесная энциклопедия

Космические корабли и станции, автоматические КА и методы их проектирования, бортовые комплексы управления, системы и средства жизнеобеспечения, особенности технологии производства ракетно-космических систем

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Мониторинг СМИ

Мониторинг СМИ и социальных сетей. Сканирование интернета, новостных сайтов, специализированных контентных площадок на базе мессенджеров. Гибкие настройки фильтров и первоначальных источников.

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Поддерживает ввод нескольких поисковых фраз (по одной на строку). При поиске обеспечивает поддержку морфологии русского и английского языка
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Применить Всего найдено 37. Отображено 37.
19-08-2016 дата публикации

ｍｉＲ－１５０의 발현을 조절하여 자연살해세포의 세포독성을 증가시키는 방법

Номер: KR0101649489B1
Автор: 최인표, 김태돈
Принадлежит: 한국생명공학연구원

... 본 발명은 miR-150의 발현을 억제하는 단계를 포함하는 자연살해세포의 세포독성 증가방법 또는 miR-150 결여된 자연살해세포의 암의 예방 또는 치료용 약학적 조성물에 관한 것으로, 구체적으로 miR-150은 Prf1 mRNA의 3'UTR과 상보적으로 결합하여 자연살해세포의 Prf1 단백질의 발현을 억제하여, miR-150의 발현을 억제하는 단계를 포함하는 자연살해세포의 세포독성을 증가시키는 방법과, miR-150 결여된 자연살해세포를 이용한 암 예방 또는 치료용 약학적 조성물에 유용하게 이용될 수 있다.

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Номер записи: 1
25-04-2019 дата публикации

PHARMACEUTICAL COMPOSITION COMPRISING MIR-150 INHIBITOR AS EFFECTIVE INGREDIENT FOR IMMUNOPOTENTIATION AND METHOD FOR SCREENING IMMUNOPOTENTIATOR

Номер: WO2019031923A3
Автор: KIM, Tae-Don, HA, Sang Jun, CHOI, In Pyo, BAN, Young Ho, SEO, Sang-Hwan, JUNG, Cho Rok
Принадлежит:

The present invention relates to a pharmaceutical composition comprising a miR-150 inhibitor as an effective ingredient for immunopotentiation and a method for screening an immunopotentiator. In addition, the present invention provides a method for screening an immunopotentiator capable of enhancing an immune response by regulating an expression level of miR-150. Through embodiments, the present invention revealed the enhancement of miR-150-deficient mice in inducing differentiation into memory CD8+ T cells. It was also revealed that memory CD8+ T cell differentiation was induced in miR-150-deficient mice, regardless of kinds of the pathogens. Therefore, a composition comprising a miR-150 inhibitor as an effective ingredient can be used as an immunopotentiator, finding effective applications in the prevention or treatment of cancer or infectious diseases.

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Номер записи: 2
23-05-2019 дата публикации

METHOD FOR PRODUCING NATURAL KILLER CELL AND USE THEREOF

Номер: WO2019059713A3
Автор: CHO, Yee Sook, KIM, Han-Seop, SEOL, Binna, CHOI, In Pyo
Принадлежит:

The present invention relates to a method for producing natural killer cells, using direct reprograming, natural killer cells produced thereby, a biomarker specific for the natural killer cells, a cell therapy product comprising the natural killer cells, a composition for treatment and prevention of cancer, comprising the natural killer cells, a cryopreservation cell vial for storing the natural killer cells, and a medium kit for inducing the direct reprogramming. Exhibiting excellent proliferative potential and cancer cell killing potential, the natural killer cells produced using the production method can be effectively utilized for mass production and in a composition for treatment and prevention of cancer.

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Номер записи: 3
13-07-2018 дата публикации

NATURAL KILLER CELL EXPRESSING ANTI-COTININE CHIMERIC ANTIGEN RECEPTOR

Номер: KR1020180081010A
Автор: CHOI IN PYO, KIM TAE DON, LEE SU UI, LEE SOO YUN, CHUNG JUNHO, KIM KI HYUN
Принадлежит:

The present invention relates to a natural killer cell expressing an anti-cotinine chimeric antigen receptor (CAR) specifically binding to cotinine, and a cell therapy agent containing the same. According to the present invention, the natural killer cell expressing the CAR specifically binding to cotinine can effectively migrate to tumor tissues regardless of types of cancer depending on a conjugate bound to cotinine. Therefore, the natural killer cell, according to the present invention, can be effectively used as a gene therapy method exhibiting highly efficiency in anti-cancer effects. COPYRIGHT KIPO 2018 (AA) NK cell ...

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Номер записи: 4
04-08-2016 дата публикации

METHOD FOR MASS PRODUCING NATURAL KILLER CELL AND USE OF NATURAL KILLER CELL OBTAINED BY THE METHOD AS ANTI-CANCER AGENT

Номер: WO2016122147A1
Автор: CHOI, In Pyo, YOON, Suk Ran, LEE, Sooyun
Принадлежит:

The present invention relates to a method for mass producing a natural killer cell and the use of the natural killer cell obtained by the method as an anti-cancer agent. By using the method of the present invention, a fresh NK cell having a high purity may be obtained within a faster time compared to conventional methods, and further, a refrigerated NK cell and a frozen NK cell having the same effect as the fresh NK cell may be produced. Moreover, an NK cell having the same effect as the fresh NK cell may be produced from a frozen CD3 negative cell. The fresh NK cell, the refrigerated NK cell and the frozen NK cell produced by means of the method of the present invention exhibit treatment effects for various cancers including colorectal cancer, lung cancer, liver cancer, pancreatic cancer and leukemia, and thus may be usefully applied as cell therapeutic agents. Further, in the present invention, a usage and a dose are established which exhibit an excellent effect when using the fresh NK ...

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Номер записи: 5
12-07-2018 дата публикации

ANTI-CEACAM6 CHIMERIC ANTIGEN RECEPTOR SPECIFICALLY BINDING TO CEACAM6

Номер: WO2018128486A1
Автор: CHOI, In Pyo, KIM, Tae-Don, SEO, Hui Yun, LEE, Sooyun, HONG, Kwon Pyo
Принадлежит:

The present invention relates to an anti-CEACAM6 chimeric antigen receptor (CAR) specifically binding to CEACAM6, and a cell therapeutic agent comprising same. The chimeric antigen receptor according to the present invention is characterized by specifically binding to CEACAM6 and can be a useful target for a targeted anticancer agent. Accordingly, the chimeric antigen receptor according to the present invention can induce immunocytes to move effectively to tumor tissue and be usefully employed in a gene and cell therapy exhibiting highly efficient anticancer effects.

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Номер записи: 6
26-03-2025 дата публикации

인체 말초 혈액의 자연살해세포 보다 더 높은 수준의 NCR, 살상능 및 IFN-γ 생산능력을 갖는 유사기억 자연살해세포의 제조 방법

Номер: KR102787753B1
Автор: 최인표, 박수연, 윤석락, 김한나, 정솔지
Принадлежит: 한국생명공학연구원

The present disclosure relates to a method for producing of a memory-like natural killer cell having an ability to produce a higher level of a natural killer cell receptor, having a better killing capacity, and having an ability to produce a higher level of IFN-γ than a natural killer cell in human peripheral blood, and a memory-like natural killer cell produced by the method, and a cancer treatment method using the memory-like natural killer cell.

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Номер записи: 7
14-02-2019 дата публикации

PHARMACEUTICAL COMPOSITION COMPRISING MIR-150 INHIBITOR AS EFFECTIVE INGREDIENT FOR IMMUNOPOTENTIATION AND METHOD FOR SCREENING IMMUNOPOTENTIATOR

Номер: WO2019031923A2
Автор: KIM, Tae-Don, HA, Sang Jun, CHOI, In Pyo, BAN, Young Ho, SEO, Sang-Hwan, JUNG, Cho Rok
Принадлежит:

The present invention relates to a pharmaceutical composition comprising a miR-150 inhibitor as an effective ingredient for immunopotentiation and a method for screening an immunopotentiator. In addition, the present invention provides a method for screening an immunopotentiator capable of enhancing an immune response by regulating an expression level of miR-150. Through embodiments, the present invention revealed the enhancement of miR-150-deficient mice in inducing differentiation into memory CD8+ T cells. It was also revealed that memory CD8+ T cell differentiation was induced in miR-150-deficient mice, regardless of kinds of the pathogens. Therefore, a composition comprising a miR-150 inhibitor as an effective ingredient can be used as an immunopotentiator, finding effective applications in the prevention or treatment of cancer or infectious diseases.

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Номер записи: 8
01-02-2017 дата публикации

자연살해세포의 대량생산 방법 및 상기 방법으로 수득된 자연살해세포의 함암제로서의 용도

Номер: KR1020170010865A
Автор: 최인표, 윤석란, 이수연
Принадлежит:

... 본 발명은 자연살해세포의 대량생산 방법 및 상기 방법으로 수득된 자연살해세포의 항암제로서의 용도에 관한 것이다. 본 발명의 방법을 이용하면 신선 NK 세포를 종래의 방법보다 빠른 시간 내에 고순도로 수득할 수 있으며, 또한 신선 NK 세포와 동등한 효능을 갖는 냉장보관 NK 세포, 냉동보관 NK 세포를 제조할 수 있다. 더 나아가 냉동보관된 CD3 음성세포로부터 신선 NK 세포와 동등한 효능을 갖는 NK 세포를 제조할 수 있다. 본 발명의 방법에 의해 제조된 신선 NK 세포, 냉장보관 NK 세포, 및 냉동보관 NK 세포는 대장암, 폐암, 간암, 췌장암 및 백혈병을 포함하는 다양한 암에 대해 치료 효과를 나타내어 세포치료제로서 유용하게 사용될 수 있다. 또한 본 발명에서는 본 발명의 신선 NK 세포, 냉장보관 NK 세포, 및 냉동보관 NK 세포를 세포치료제 약학적 조성물로 사용할 때 우수한 효과를 나타내는 용법 및 용량을 확립하였다.

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Номер записи: 9
11-06-2020 дата публикации

ANTI-CANCER COMPOSITION COMPRISING IN VIVO CELL INJECTION CHIP

Номер: WO2020116989A1
Автор: KIM, Tae Don, CHOI, In Pyo, LIM, Yong Taik, AHN, Young Ha, JUNG, Cho Rok, KIM, Da Seul, KIM, Seokmin
Принадлежит:

The present invention relates to an anti-cancer pharmaceutical composition comprising a vivo cell injection chip as an active ingredient, the composition comprising: a porous three-dimensional cryogel scaffold comprising a structure in which first and second components of a hyaluronic acid derivative, having different chemical structures, are crosslinked; and cells cultured in chambers of the scaffold. The composition comprising the scaffold and the cells cultured in chambers of the scaffold exhibits a cell growth rate, a survival rate, a cancer cell killing ability, and a cancer metastasis reduction effect that is greater than those of a conventional two-dimensional culture, thereby being effectively usable in cancer treatment and cancer treatment methods or as a composition for cell cultures.

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Номер записи: 10
10-07-2015 дата публикации

A method of differentiating stem cells into IL-22-producing natural killer cells

Номер: KR0101535172B1
Автор: 최인표, 김미선, 김원삼
Принадлежит: 한국생명공학연구원

... 본 발명은 줄기세포로부터 IL-22를 생산하는 자연살해세포 분화 방법에 관한 것으로, 보다 구체적으로 골수로부터 유래 된 조혈 줄기세포로부터 유도된 전구체 세포에 낮은 농도의 IL-15(Interleukin-15) 및 IL-23(Interleukin-23)을 혼합 처리함으로써, IL-22을 생산하는 NK-22 세포로의 분화를 유도할 수 있으므로 상기 유도된 NK-22를 이용하여 염증, 간염 및 암 등의 질환 치료에 유용하게 이용될 수 있다.

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Номер записи: 11
31-12-2015 дата публикации

SAFETY PNEUMATIC SYSTEM FOR PREVENTING ACCIDENTAL DISCHARGE

Номер: KR1020150145993A
Автор: KIM, SUNG KWANG, CHOI, IN PYO, JANG, BONG KI, KWON, JOON SIK
Принадлежит:

The present invention relates to a safety pneumatic system for preventing accidental discharge, which is managed in a warship, and a launching pad having the same. Provided are a safety pneumatic system for preventing accidental discharge and a launching pad having the same. The safety pneumatic system for preventing accidental discharge comprises: a valve housing having an inlet, a first outlet, and a second outlet; a three-way valve installed inside the valve housing; a switch connected to the three-way valve to control opening and closing of the three-way valve; a first pneumatic line connected to the inlet; and a second pneumatic line connected to the first outlet. COPYRIGHT KIPO 2016 ...

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Номер записи: 12
07-09-2012 дата публикации

DIFFERENTIATION AND PROLIFERATION METHOD FOR NATURAL KILLER CELLS FROM CD14-POSITIVE MONOCYTES

Номер: WO2012118349A3
Автор: CHOI, In Pyo, YOON, Suk Ran, KIM, Mi-Joung
Принадлежит:

The present invention relates to an effective differentiation and proliferation method for natural killer cells (NK cells) from cord blood, and more specifically, to a method for effectively differentiating and proliferating natural killer cells from cord blood, comprising the following steps: 1) removing CD3, CD34, CD56 and CD19 positive cells from cord blood-derived monocytes to prepare CD3-CD34-CD56-CD19 cells; 2) removing CD14-negative cells from the CD3-CD34-CD56-CD19 cells to prepare CD3-CD34-CD56-CD19-CD14-positive cells; and 3) mixing the CD3-CD34-CD56-CD19-CD14-positive cells with IL-15 and IL-21 and culturing the mixture. The present invention can obtain NK cells with a high purity within a short time by inducing NK cells from the CD14-positive cells, compared with a method inducing NK cells from hematopoietic stem cells. In addition, the present invention can induce NK cells with an excellent cell killing activity within a short time so as to differentiate the same into NK cells ...

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Номер записи: 13
04-08-2016 дата публикации

METHOD FOR MASS-PRODUCING NATURAL KILLER CELLS AND USE OF NATURAL KILLER CELLS OBTAINED BY THE METHOD AS ANTICANCER AGENT

Номер: WO2016122014A1
Автор: CHOI, In Pyo, YOON, Suk Ran, LEE, Sooyun
Принадлежит:

The present invention relates to a method for mass-producing natural killer cells and a use of the natural killer cells obtained by the method as an anticancer agent. NK cells produced according to the mass-production method of the present invention has been confirmed to be useful in a pharmaceutical composition for preventing and treating cancer by confirming that, when CD3-negative cells are obtained by removing CD3-positive T-cells from monocytes and the CD3-negative cells are treated by mixing with cytokines such as IL-15 and IL-21 and then cultivated, highly-pure NK cells could be mass-produced within a shorter amount of time, compared to existing methods for producing natural killer cells, the NK cells produced by the method inhibited growth of cancer cells and reduced the weight of cancer cells and had a therapeutic effect even in blood cancer such as leukemia in a mouse model in which cell strains of colon cancer, lung cancer, liver cancer, and pancreatic cancer have been xenotransplanted ...

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Номер записи: 14
07-09-2012 дата публикации

DIFFERENTIATION AND PROLIFERATION METHOD FOR NATURAL KILLER CELLS FROM CD14-POSITIVE MONOCYTES

Номер: WO2012118349A9
Автор: CHOI, In Pyo, YOON, Suk Ran, KIM, Mi-Joung
Принадлежит:

The present invention relates to an effective differentiation and proliferation method for natural killer cells (NK cells) from cord blood, and more specifically, to a method for effectively differentiating and proliferating natural killer cells from cord blood, comprising the following steps: 1) removing CD3, CD34, CD56 and CD19 positive cells from cord blood-derived monocytes to prepare CD3-CD34-CD56-CD19 cells; 2) removing CD14-negative cells from the CD3-CD34-CD56-CD19 cells to prepare CD3-CD34-CD56-CD19-CD14-positive cells; and 3) mixing the CD3-CD34-CD56-CD19-CD14-positive cells with IL-15 and IL-21 and culturing the mixture. The present invention can obtain NK cells with a high purity within a short time by inducing NK cells from the CD14-positive cells, compared with a method inducing NK cells from hematopoietic stem cells. In addition, the present invention can induce NK cells with an excellent cell killing activity within a short time so as to differentiate the same into NK cells ...

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Номер записи: 15
30-07-2015 дата публикации

DIRECT DEDIFFERENTIATION METHOD OF HEMATOPOIETIC STEM CELL FROM FIBROBLAST CELL

Номер: KR1020150087727A
Автор: CHOI, IN PYO, SONG, HAE YOUNG
Принадлежит:

The present invention relates to a direct dedifferentiation inducing method of a hematopoietic stem cell from a fibroblast cell. More specifically, the method includes the steps of selecting a dedifferentiation inducing candidate gene and initiating direct dedifferentiation to the induced hematopoietic stem cell having a multi-differentiation capability through the overexpression of the differentiation inducing candidate gene from a mouse embryo fibroblast. The present invention confirms the differentiation from the dedifferentiation inducing hematopoietic stem cell to a lymphoid cell and a myeloid cell, thus the direct dedifferentiation inducing method of a hematopoietic stem cell can be utilized for patient-specific cell treatment. COPYRIGHT KIPO 2015 ...

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Номер записи: 16
05-05-2011 дата публикации

METHOD FOR ACTIVATING A NATURAL KILLER CELL BY ADJUSTING THE EXPRESSION OF THE SOCS2 GENE

Номер: KR2011052883A2
Автор: CHOI, Inpyo, LEE, Suk Hyung, YOON, Suk-Ran
Принадлежит:

The present invention relates to a method for activating a natural killer cell (NK cell), and more particularly, to a method for increasing the cytotoxicity of the natural killer cell by inducing the overexpression of suppressor of cytokine signaling 2 (SOCS2) which is a protein involved in a cell-signaling process in the natural killer cell. The inventor of the present invention has found that the expression of SOCS2 increased in the event the natural killer cell was treated with interleukin 15 (IL-15), which is a cytokine involved in the differentiation of the natural killer cell, and also has found that the expression of proline-rich tyrosine kinase 2 (Pyk2) was inhibited by the SOCS2, the expression of which increased. In addition, the ability of producing interferon-γ (IFN-γ) and the oncolytic activity of the natural killer cell decreased in the event of an overexpression of Pyk2, and therefore SOCS2 can be effectively used in activating the natural killer cell. The natural killer ...

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Номер записи: 17
12-05-2011 дата публикации

ANTIVIRAL COMPOSITION CONTAINING AN ALEURITES FORDII OR DAPHNE KIUSIANA EXTRACT OR A FRACTION THEREOF AS AN ACTIVE INGREDIENT

Номер: KR2011055979A2
Автор: OH, Sei-Ryang, KIM, Jae Wha, LEE, Hyeong-Kyu, AHN, Kyung Seop, KIM, Doo Young, KIM, Jung Hee, SONG, Jae Sung, KANG, Ho-Bum, CHOI, Inpyo
Принадлежит:

The present invention relates to an antiviral composition containing an Aleurites fordii or Daphne kiusiana extract or a fraction thereof as an active ingredient. In detail, an Aleurites fordii or Daphne kiusiana extract or a fraction thereof can induce the secretion of interferon-γ (IFN-γ) which is an immune-related cytokine in a natural killer (NK) cell, and thus exhibits high antiviral activity and superior effects for viral diseases, and therefore can be effectively used in an antiviral composition.

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Номер записи: 18
13-07-2018 дата публикации

ANTI-CEACAM6 CHIMERIC ANTIGEN RECEPTOR SPECIFICALLY BINDING TO CEACAM6

Номер: KR1020180081011A
Автор: CHOI IN PYO, KIM TAE DON, SEO HUI YUN, LEE SOO YUN, HONG KWON PYO
Принадлежит:

The present invention relates to an anti-CEACAM6 chimeric antigen receptor (CAR) specifically binding to CEACAM6, and to a cell therapeutic agent comprising the same. The CAR according to the present invention is characterized by specifically binding to CEACAM6 and can be a useful target for a targeted anticancer agent. Accordingly, the CAR according to the present invention can induce immunocytes to move effectively to tumor tissue and be usefully employed in a gene and cell therapy exhibiting highly efficient anticancer effects. COPYRIGHT KIPO 2018 ...

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Номер записи: 19
30-03-2018 дата публикации

COMPOSITION CONTAINING PEPTIDE DERIVED FROM THIOREDOXIN-INTERACTING PROTEIN OR POLYNUCLEOTIDE ENCODING SAME AS ACTIVE INGREDIENT FOR REVERSE AGING OF AGED STEM CELLS, AND USE THEREOF

Номер: KR1020180032506A
Автор: CHOI, IN PYO, JUNG, HAI YOUNG, KIM, DONG OH, KIM, MI JEONG, BYUN, JAE EUN
Принадлежит:

The present invention relates to a pharmaceutical composition for reverse aging of aged stem cells, wherein the pharmaceutical composition contains a peptide derived from thioredoxin-interacting protein (TXNIP) or a polynucleotide encoding the same as an active ingredient. It has been confirmed that the peptide derived from TXNIP increases engraftment of aged hematopoietic stem cells (HSC), deceases a serial biasing tendency, decreases a long-term HSC (LT-HSC) ratio, and suppresses p38 and ROS in a mouse in which competitive transplantation is performed by using aged HSCs compared to a control group which is not treated with a peptide. Thus, it has been confirmed that the characteristics of aged HSCs are suppressed, and it has been confirmed that administration of a peptide derived from TXNIP increases the proliferation of leukocytes of an aged mouse to a level of a young mouse in an acute leucopenia model. In addition, it has been confirmed that the peptide derived from TXNIP recovers ...

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Номер записи: 20
18-12-2014 дата публикации

PHARMACEUTICAL COMPOSITION CONTAINING THIOREDOXIN-BINDING PROTEIN AS ACTIVE INGREDIENT AND USE THEREOF

Номер: WO2014200180A1
Автор: CHOI, In Pyo, JUNG, Haiyoung, KIM, Mi jung
Принадлежит:

The present invention relates to a composition, containing thioredoxin-binding protein (TXNIP) as an active ingredient, for boosting immunity, inhibiting cancer metastasis, and preventing and treating blood diseases. More specifically, it is confirmed that the frequency of hematopoietic stem cells and myeloproliferative ability decrease in TXNIP-deficient mice, and that the myeloproliferative ability of marrow cells, which are derived from the TXNIP-deficient mice, decreases through bone-marrow transplantation. Additionally, it is confirmed that the level of reactive oxygen species increases and apoptosis is induced in TXNIP knockout mice and TXNIP-deficient cell strains, under oxidative stress conditions, and that the myeloproliferative ability of the cell strains derived from the TXNIP knockout mice decreases. Furthermore, it is confirmed that cancer metastasis increases and p53 expression decreases in the TXNIP knockout mice under oxidative stress conditions. Moreover, direct binding ...

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Номер записи: 21
15-02-2016 дата публикации

Safety pneumatic system for preventing accidental discharge

Номер: KR0101593635B1
Автор: 김성광, 최인표, 장봉기, 권준식
Принадлежит: 국방기술품질원

... 본 발명은 함정에서 운용하는 오발사 방지용 안전 공압 시스템 및 이를 포함하는 발사대에 관한 것으로, 유입구, 제1유출구, 제2유출구를 구비하는 밸브 하우징; 밸브 하우징 내부에 설치되는 3-방향 밸브; 3-방향 밸브와 연결되어 3-방향 밸브의 개폐를 제어하는 스위치; 유입구와 연결되는 제1공압라인; 및 제1유출구와 연결되는 제2공압라인을 포함하는 오발사 방지용 안전 공압 시스템 및 이를 포함하는 발사대를 제공한다.

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Номер записи: 22
28-03-2019 дата публикации

METHOD FOR PRODUCING NATURAL KILLER CELL AND USE THEREOF

Номер: WO2019059713A2
Автор: CHO, Yee Sook, KIM, Han-Seop, SEOL, Binna, CHOI, In Pyo
Принадлежит:

The present invention relates to a method for producing natural killer cells, using direct reprograming, natural killer cells produced thereby, a biomarker specific for the natural killer cells, a cell therapy product comprising the natural killer cells, a composition for treatment and prevention of cancer, comprising the natural killer cells, a cryopreservation cell vial for storing the natural killer cells, and a medium kit for inducing the direct reprogramming. Exhibiting excellent proliferative potential and cancer cell killing potential, the natural killer cells produced using the production method can be effectively utilized for mass production and in a composition for treatment and prevention of cancer.

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Номер записи: 23
22-09-2015 дата публикации

METHOD FOR INCREASING CYTOTOXICITY OF NATURAL KILLER CELL BY REGULATING MIR-150 EXPRESSION

Номер: KR1020150106543A
Автор: CHOI, IN PYO, KIM, TAE DON
Принадлежит:

The present invention relates to a method for increasing cytotoxicity of natural killer cells including a step for inhibiting miR-150 expression or a pharmaceutical composition for preventing or treating cancer through miR-150-deficient natural killer cells and, specifically, to a method for increasing cytotoxicity of natural killer cells including a step for inhibiting miR-150 expression, which inhibits Prf1 protein expression of natural killer cells by using miR-150 which is complementarily bonded to 3′UTR of Prf1 mRNA, and to a pharmaceutical composition for preventing or treating cancer using miR-150-deficient natural killer cells. COPYRIGHT KIPO 2015 (AA) Wild type ...

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Номер записи: 24
05-05-2011 дата публикации

METHOD FOR ACTIVATING A NATURAL KILLER CELL BY ADJUSTING THE EXPRESSION OF THE SOCS2 GENE

Номер: WO2011052883A3
Автор: CHOI, Inpyo, LEE, Suk Hyung, YOON, Suk-Ran
Принадлежит:

The present invention relates to a method for activating a natural killer cell (NK cell), and more particularly, to a method for increasing the cytotoxicity of the natural killer cell by inducing the overexpression of suppressor of cytokine signaling 2 (SOCS2) which is a protein involved in a cell-signaling process in the natural killer cell. The inventor of the present invention has found that the expression of SOCS2 increased in the event the natural killer cell was treated with interleukin 15 (IL-15), which is a cytokine involved in the differentiation of the natural killer cell, and also has found that the expression of proline-rich tyrosine kinase 2 (Pyk2) was inhibited by the SOCS2, the expression of which increased. In addition, the ability of producing interferon-γ (IFN-γ) and the oncolytic activity of the natural killer cell decreased in the event of an overexpression of Pyk2, and therefore SOCS2 can be effectively used in activating the natural killer cell. The natural killer ...

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Номер записи: 25
12-04-2012 дата публикации

COMPOSITION FOR NATURAL KILLER CELL DIFFERENTIATION CONTAINING A TOX AS AN ACTIVE INGREDIENT

Номер: KR2012046940A1
Автор: CHOI, In Pyo, YUN, So Hyun, YOON, Suk-Ran
Принадлежит:

The present invention relates to a composition for natural killer cell differentiation containing a TOX (thymocyte selection-associated HMG box protein) as an active ingredient and to a differentiation method using the same, and, more specifically, the TOX can be used to advantage in inducing natural killer cell differentiation since TOX regulates the expression of T-bet which induces differentiation from haematopoietic stem cells to natural killer cells and is known to be an important regulator in the process of differentiation of natural killer cells.

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Номер записи: 26
29-04-2010 дата публикации

METHOD FOR EFFICIENTLY PROLIFERATING AND DIFFERENTIATING NATURAL KILLER CELLS FROM UMBILICAL CORD BLOOD

Номер: KR2010047475A2
Автор: CHOI, Inpyo, YOON, Suk-Ran, LEE, Soo Yun, LEE, Joo-Yong, LIM, Jaeseung
Принадлежит:

The present invention relates to a method for efficiently proliferating and differentiating natural killer cells (NK cells) from umbilical cord blood. More specifically, the present invention relates to a method comprising 1) a step of removing CD3-positive T-cells from cord blood-derived monocytes to obtain CD3-negative cells; and 2) a step of mixing and treating various cytokines to CD3-negative cells and culturing the mixture. The invention is able to obtain NK cells of high purity in a short time by inducing NK cells from CD3-negative cells compared to a method for inducing NK cells from hematopoietic stem cells. Also, the proliferation and differentiation of NK cells are promoted by mixing and treating IL-15 and IL-21, from which NK cells with enhanced cytotoxicity are induced. Therefore, since NK cells having the ability to kill cancer cells can be differentiated, they can be useful in cancer cell therapy.

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Номер записи: 27
02-03-2016 дата публикации

Pharmaceutical composition comprising Thioredoxin-binding protein as an active ingradient and using thereof

Номер: KR0101598905B1
Автор: 최인표, 정해용, 김미정
Принадлежит: 한국생명공학연구원

... 본 발명은 티오레독신-결합 단백질(thioredoxin-binding protein; TXNIP)을 유효성분으로 포함하는 면역증강, 암 전이억제, 및 혈액질환 예방 및 치료용 조성물에 관한 것으로, 보다 구체적으로, TXNIP 결핍 마우스에서 조혈모세포의 빈도가 감소하고, 골수증식 능력이 감소하며, 골수이식을 통해 TXNIP 결핍 마우스-유래 골수세포의 증식능력이 감소하는 것을 확인하였다. 또한, 산화적 스트레스 조건하의 TXNIP 녹아웃 마우스 및 TXNIP 결핍 세포주에서 활성산소의 수준이 증가하고, 세포사멸을 유도하며, 상기 TXNIP 녹아웃 마우스 유래 세포주의 골수증식 능력이 감소하는 것을 확인하였다. 또한, 산화적 스트레스 조건하의 TXNIP 녹아웃 마우스에서 암 전이가 증가하는 것을 확인하였고, p53의 발현이 감소함을 확인하였다. 또한, TXNIP 및 p53 사이에 직접적인 결합 위치를 확인하였고, 상기 결합이 산화적 스트레스 조건하에서 증가하며, 이로 인하여 p53-매개의 전사활성이 증가하고, 또한, p53-매개 항산화활성이 증가하는 것을 확인함으로써 본 발명의 TXNIP 유전자를 포함하는 유전자 전달체, 세포 또는 TXNIP 단백질이 면역증강, 암 전이억제, 및 혈액질환 예방 및 치료용 조성물로 유용하게 사용될 수 있다.

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Номер записи: 28
29-04-2010 дата публикации

METHOD FOR EFFICIENTLY PROLIFERATING AND DIFFERENTIATING NATURAL KILLER CELLS FROM UMBILICAL CORD BLOOD

Номер: WO2010047475A3
Автор: CHOI, Inpyo, YOON, Suk-Ran, LEE, Soo Yun, LEE, Joo-Yong, LIM, Jaeseung
Принадлежит:

The present invention relates to a method for efficiently proliferating and differentiating natural killer cells (NK cells) from umbilical cord blood. More specifically, the present invention relates to a method comprising 1) a step of removing CD3-positive T-cells from cord blood-derived monocytes to obtain CD3-negative cells; and 2) a step of mixing and treating various cytokines to CD3-negative cells and culturing the mixture. The invention is able to obtain NK cells of high purity in a short time by inducing NK cells from CD3-negative cells compared to a method for inducing NK cells from hematopoietic stem cells. Also, the proliferation and differentiation of NK cells are promoted by mixing and treating IL-15 and IL-21, from which NK cells with enhanced cytotoxicity are induced. Therefore, since NK cells having the ability to kill cancer cells can be differentiated, they can be useful in cancer cell therapy.

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Номер записи: 29
29-03-2018 дата публикации

COMPOSITION COMPRISING THIOREDOXIN-INTERACTING PROTEIN-DERIVED PEPTIDE OR POLYNUCLEOTIDE ENCODING SAME AS EFFECTIVE INGREDIENT FOR REVERSE-AGEING OF AGED STEM CELL AND USE THEREOF

Номер: WO2018056706A1
Автор: CHOI, In Pyo, JUNG, Haiyoung, KIM, Dong Oh, KIM, Mi Jeong, BYUN, Jae Eun
Принадлежит:

The present invention relates to a pharmaceutical composition comprising a thioredoxin-interacting protein (TXNIP)-derived peptide or a polynucleotide coding therefor as an effective ingredient for reverse ageing of stem cells. In mice competitively transplanted with aged hematopoietic stem cells (HSC), a TXNIP-derived peptide was observed to increase the engraftment of aged HSC, decrease lineage skewing, reduce a long-term HSC (LT-HSC) ratio, and inhibit p38 and ROS, thus suppressing features of aged HSC, compared to a control untreated with the peptide. In acute leukopenia models, administration of the TXNIP-derived peptide was found to increase the leukocyte proliferation of aged mice to a level of young mice. In addition, it was found that the TXNIP-derived peptide causes recovery from Cdc42 polarization in aged HSC, downregulates the expression of the senescence-associated genes p16, p19, p21, and Wnt5a, and increases the homing of HSC. Therefore, the TXNIP-derived peptide of the present ...

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Номер записи: 30
12-05-2011 дата публикации

ANTIVIRAL COMPOSITION CONTAINING AN ALEURITES FORDII OR DAPHNE KIUSIANA EXTRACT OR A FRACTION THEREOF AS AN ACTIVE INGREDIENT

Номер: WO2011055979A3
Автор: OH, Sei-Ryang, KIM, Jae Wha, LEE, Hyeong-Kyu, AHN, Kyung Seop, KIM, Doo Young, KIM, Jung Hee, SONG, Jae Sung, KANG, Ho-Bum, CHOI, Inpyo
Принадлежит:

The present invention relates to an antiviral composition containing an Aleurites fordii or Daphne kiusiana extract or a fraction thereof as an active ingredient. In detail, an Aleurites fordii or Daphne kiusiana extract or a fraction thereof can induce the secretion of interferon-γ (IFN-γ) which is an immune-related cytokine in a natural killer (NK) cell, and thus exhibits high antiviral activity and superior effects for viral diseases, and therefore can be effectively used in an antiviral composition.

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Номер записи: 31
12-07-2018 дата публикации

NATURAL KILLER CELL EXPRESSING ANTI-COTININE CHIMERIC ANTIGEN RECEPTOR

Номер: WO2018128485A1
Автор: CHOI, In Pyo, KIM, Tae-Don, LEE, Su Ui, LEE, Sooyun, CHUNG, Junho, KIM, Ki-Hyun
Принадлежит:

The present invention relates to a natural killer cell expressing an anti-cotinine chimeric antigen receptor (CAR) specifically binding to cotinine, and a cell therapeutic agent comprising same. The natural killer cell expressing a chimeric antigen receptor specifically binding to cotinine, according to the present invention, can effectively move to tumor tissue, regardless of the kind of cancer, depending on the binding substance bound to cotinine. Therefore, the natural killer cell according to the present invention can be usefully employed as a gene therapy exhibiting a highly efficient anticancer effect.

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Номер записи: 32
05-05-2011 дата публикации

METHOD FOR ACTIVATING A NATURAL KILLER CELL BY ADJUSTING THE EXPRESSION OF THE SOCS2 GENE

Номер: KR2011052883A9
Автор: CHOI, Inpyo, LEE, Suk Hyung, YOON, Suk-Ran
Принадлежит:

The present invention relates to a method for activating a natural killer cell (NK cell), and more particularly, to a method for increasing the cytotoxicity of the natural killer cell by inducing the overexpression of suppressor of cytokine signaling 2 (SOCS2) which is a protein involved in a cell-signaling process in the natural killer cell. The inventor of the present invention has found that the expression of SOCS2 increased in the event the natural killer cell was treated with interleukin 15 (IL-15), which is a cytokine involved in the differentiation of the natural killer cell, and also has found that the expression of proline-rich tyrosine kinase 2 (Pyk2) was inhibited by the SOCS2, the expression of which increased. In addition, the ability of producing interferon-γ (IFN-γ) and the oncolytic activity of the natural killer cell decreased in the event of an overexpression of Pyk2, and therefore SOCS2 can be effectively used in activating the natural killer cell. The natural killer ...

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Номер записи: 33
01-08-2013 дата публикации

COMPOSITION CONTAINING TANSHINONE AS ACTIVE INGREDIENT, FOR INCREASING DIFFERENTIATION OR ACTIVITY OF NATURAL KILLER CELLS

Номер: WO2013111969A1
Автор: CHOI, In Pyo, KIM, Won Sam, KWON, Byoung-Mog
Принадлежит:

The present invention relates to a composition containing tanshinone as an active ingredient, for increasing a differentiation or activity of natural killer (NK) cells. More particularly, the present invention relates to a composition in which cryptotanshinone or tanshinone IIA induces and promotes a differentiation of NK cells and increases an expression of ID2 which is a gene relating to an NK cell differentiation in hematopoietic stem cells. The cryptotanshinone or tanshinone IIA has cytotoxicity to YAC-1, which is a cancer cell, and also increases generation of IFN-γ, thus increasing the activity of NK cells. Therefore, the cryptotanshinone or tanshinone IIA can be effectively used as an active ingredient for a composition for increasing a differentiation or activity of NK cells.

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Номер записи: 34
07-09-2012 дата публикации

DIFFERENTIATION AND PROLIFERATION METHOD FOR NATURAL KILLER CELLS FROM CD14-POSITIVE MONOCYTES

Номер: WO2012118349A2
Автор: CHOI, In Pyo, YOON, Suk Ran, KIM, Mi-Joung
Принадлежит:

The present invention relates to an effective differentiation and proliferation method for natural killer cells (NK cells) from cord blood, and more specifically, to a method for effectively differentiating and proliferating natural killer cells from cord blood, comprising the following steps: 1) removing CD3, CD34, CD56 and CD19 positive cells from cord blood-derived monocytes to prepare CD3-CD34-CD56-CD19 cells; 2) removing CD14-negative cells from the CD3-CD34-CD56-CD19 cells to prepare CD3-CD34-CD56-CD19-CD14-positive cells; and 3) mixing the CD3-CD34-CD56-CD19-CD14-positive cells with IL-15 and IL-21 and culturing the mixture. The present invention can obtain NK cells with a high purity within a short time by inducing NK cells from the CD14-positive cells, compared with a method inducing NK cells from hematopoietic stem cells. In addition, the present invention can induce NK cells with an excellent cell killing activity within a short time so as to differentiate the same into NK cells ...

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Номер записи: 35
07-09-2015 дата публикации

A method for direct dedifferentiation of hematopoietic stem cell from fibroblast cell

Номер: KR0101550269B1
Автор: 최인표, 송해영
Принадлежит: 한국생명공학연구원

... 본 발명은 섬유아세포로부터 조혈줄기세포로의 직접 역분화 유도 방법에 관한 것으로, 구체적으로 역분화 유도 후보 유전자를 선별한 후, 마우스 섬유아세포(mouse embryo fibroblast)로부터 상기 역분화 유도 후보 유전자의 과발현을 통해 다분화능을 가진 조혈줄기세포(induced hematopoietic stem cell)로 직접 역분화 되고, 상기 역분화 유도 조혈줄기세포에서 림프성 세포 및 골수성 세포로의 분화를 확인함으로써, 상기 조혈줄기세포 직접 역분화 유도 방법을 환자 특이적 세포 치료에 유용하게 이용할 수 있다.

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Номер записи: 36
25-06-2015 дата публикации

PHARMACEUTICAL COMPOSITION INCLUDING THIOREDOXIN-BINDING PROTEIN AS ACTIVE INGREDIENT AND USE THEREOF

Номер: KR1020150070990A
Автор: CHOI, IN PYO, JUNG, HAI YOUNG, KIM, MI JUNG
Принадлежит:

The present invention relates to a composition, including thioredoxin-binding protein (TXNIP) as an active ingredient, for enhancing immunity, inhibiting cancer metastasis, and preventing and treating blood diseases. More specifically, in TXNIP-deficient mice, it is confirmed that the frequency of hematopoietic stem cells and myeloproliferative ability decrease, and that the myeloproliferative ability of marrow cells derived from the TXNIP-deficient mice decreases through bone-marrow transplantation. Moreover, in TXNIP knockout mice and TXNIP-deficient cell strains under oxidative stress conditions, it is confirmed that the level of reactive oxygen species increases and apoptosis is induced, and that the myeloproliferative ability of the cell strains derived from the TXNIP knockout mice decreases. In the TXNIP knockout mice under oxidative stress conditions, it is confirmed that cancer metastasis increases and p53 expression decreases. Moreover, direct binding sites between TXNIP and p53 ...

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Номер записи: 37