10-05-2018 дата публикации
Номер: US20180127504A1
Принадлежит:
An isolated antibody or an antigen-binding fragment thereof having a specific binding affinity to an epitope located within the domain 1 or domain 3 of human vascular endothelial growth factor receptor 2 (VEGFR2; SEQ ID NO: 74) is disclosed. The epitope within the domain 3 of the VEGFR2 is located between amino acid residues 250 and 270 of SEQ ID NO: 74. Use of the antibody or antigen-binding fragment thereof in the manufacture of a medicament for inhibiting tumor growth, tumor angiogenesis, and/or inducing cancer cell cytotoxicity in a subject in need thereof is also disclosed. Also disclosed is a method of detecting the presence of VEGFR2 in a tumor vascular endothelial cell or a cancer cell in a biological sample. 1. An isolated antibody or an antigen-binding fragment thereof that has a specific binding affinity to an epitope located within the domain 1 or domain 3 of human vascular endothelial growth factor receptor 2 (VEGFR2; SEQ ID NO: 74) , wherein the epitope within the domain 3 of the VEGFR2 is located between amino acid residues 250 and 270 of SEQ ID NO: 74.2. The antibody or antigen-binding fragment thereof of claim 1 , wherein the epitope comprises the amino acid sequence of NWEYPS (SEQ ID NO: 66).3. The antibody or antigen-binding fragment thereof of claim 1 , which comprises a heavy chain variable region (V) and a light chain variable region (V) claim 1 , the Vcomprising VCDR1 claim 1 , VCDR2 claim 1 , and VCDR3 claim 1 , and the Vcomprising VCDR1 claim 1 , VCDR2 claim 1 , and VCDR3 claim 1 ,(i) wherein:{'sub': H', 'H', 'H', 'L', 'L', 'L, 'the VCDR1, VCDR2, and VCDR3 comprise the amino acid sequence of SEQ ID NO: 1, SEQ ID NO: 2, and SEQ ID NO: 3, respectively; and the VCDR1, VCDR2, and VCDR3 comprise the amino acid sequence of SEQ ID NO: 4, Ala Ala Ser, and SEQ ID NO: 5, respectively;'}or (ii) wherein:{'sub': H', 'H', 'H', 'L', 'L', 'L, 'the VCDR1, VCDR2, VCDR3 comprise the amino acid sequence of SEQ ID NO: 6, SEQ ID NO: 7, and SEQ ID NO: 8, ...
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