СПОСОБ ПОЛУЧЕНИЯ ЭНАНТИОСЕЛЕКТИВНОГО СОРБЕНТА

Изобретение относится к способам получения сорбентов на основе хелатов металлов, используемых для разделения энантиомеров. Способ получения энантиоселективного сорбента включает нанесение на поверхность силикагеля комплексного соединения металла, содержащего энантиоселективный фрагмент, причем используют гамма-замещенный ацетилацетонат d-металла и остаток энантиомера аминокислоты из ряда L- или D-энантиомеров аргинина, валина, триптофана, метионина, фенилаланина, треонина. Полученные сорбенты эффективны для разделения энантиомеров органических соединений, являющихся компонентами медицинских и косметических препаратов. 1 з.п. ф-лы, 1 табл.

Сорбент для жидкостной хроматографии

Изобретение относится к химически модифицированным кремнеземным сорбентам для жидкостной хроматографии и может быть использовано для разделения и анализа энантиомерного состава оптически активных соединений. геометрических изомеров металлоорга- нических соединений, а также гомоло- гов, например алкилфталатов. Сорбент на основе кремнезема содержит привитое к поверхности производное хинина формулы СН2СН2 ОСИНа предложенном сорбенте удается с высокой селективностью разделить оптические изомеры 2,2,2-трифтор-1-(9- антрил)этанола, гомологи диалкилфта- латов и изомеры металлоорганических соединений. 4 ил. 1 табл. ел ...

VERFAHREN ZUR TRENNUNG OPTISCHER UND GEOMETRISCHER ISOMERE.

NUCLEIC ACIDS IN THE FORM OF SPECIFIC NEW OF CHIRALER SELECTORS

USE MONOLITHIC SORBENTIEN FOR PRÄPARATIVE CHROMATOGRAPHI SEPARATION PROCESSES

A PROCESS FOR THE SYNTHESIS OF A CHROMATOGRAPHIC PHASE

A process for the synthesis, delivery or deposition or localisation of a chromatographic phase, especially for chromatographic separation or solid phase extraction, comprises introducing a chemical moiety to a support using a supercritical fluid such as supercritical carbon dioxide.

Method for preparing chiral recognition functional fiber

The invention relates to a preparation method of chiral recognition functional fiber. In the method, cyclodextrin is prepared into binuclear copper complex with cyclodextrin. Then, the binuclear copper complex with cyclodextrin is grafted on cellulose fiber through chemical bonds to prepare the fiber with chiral recognition function which is used for the chiral recognition and separation of D/L type aromatic amino enantiomer. Compared with the prior art, the preparation method of the chiral recognition functional fiber is characterized by low separation cost, good effect, convenient separation of products, convenient recycling of materials, etc.

Rapid preparation and application of MBA bridged cyclodextrin functionalized chiral monolithic column

A phenylalanine chiral chromatographic column stationary phase of preparation method

Nano metal chiral identification solid phase, chiral separation chromatograph and its preparation method

Preparation and application of novel CH2-N=C-Ar group-modified cyclodextrin derivatives

The invention provides a method for synthesizing a novel CH2-N=C-Ar group modified beta-cyclodextrin derivatives by utilizing beta-cyclodextrin and application of the derivative in chiral separation. The principle that a tough and flexible chiral ligand is beneficial to improving the chiral catalysis enantioselectivitiy and obtaining higher substrate adaptability is applied to the design of cyclodextrin chiral stationary phase derived structure molecules; 6-deoxy-6-amino-beta-cyclodextrin is synthesized by the synthesis step which is simpler and is easy for operation; the 6-deoxy-6-amino-beta-cyclodextrin reacts with four different aromatic aldehydes to generate four novel CH2-N=C-Ar group-containing beta-cyclodextrin derivatives; and the aldehyde reacting with cyclodextrin ammonia can bebenzaldehyde, 2, 4-dihydroxy benzaldehyde, nitro. aratrimethylaminobenzaldehyde and nitro. Para N-methylimidazolylbenzaldehyde. The preparation method provided by the invention has the advantages ofsimpleness ...

NUCLEIC ACIDS AS NEW CHIRAL SELECTORS SPECIFIC

METHOD FOR PRODUCING A CHROMATOGRAPHY MEDIUM AND CHROMATOGRAPHY MEDIUM

The present invention relates to a method to improve chromatography beads. More closely, the invention relates to a novel method for production of dextran-containing porous media and chromatography media produced with this method. In the method, the chromatography media is subjected to dextranase-treatment leading to improved pressure-flow properties of the media.

SEPARATORY AGENT FOR OPTICAL ISOMER

A separatory agent for optical isomers which has the characteristic ability to identify asymmetry. It comprises as the active ingredient an aromatic polysaccharide derivative having a C10 or higher alkoxy group as a substituent for an aromatic group.

Separation agent for separating optical isomer and method for preparation thereof

A method of producing a novel separating agent for separating enantiomeric isomers, characterized by including: a step of supporting an optically active polymer compound and a compound having an asymmetric structure of a molecular weight of 1,000 or less on a carrier using a solvent; a step of removing the solvent; and a step of removing the compound having the asymmetric structure of the molecular weight of 1,000 or less by washing.

CHIRAL INORGANIC-ORGANIC COMPOSITE POROUS MATERIAL AND METHOD FOR PREPARING THE SAME

RESOLVING AGENT FOR OPTICAL ISOMERS

ХИРАЛЬНЫЕ ОПТИЧЕСКИЕ АКТИВНЫЕ АДСОРБЕНТЫ, СПОСОБЫ ИХ ПОЛУЧЕНИЯ, СЕТЧАТЫЙ ПОЛИМЕР, ПРОИЗВОДНЫЕ ВИННОЙ КИСЛОТЫ И СПОСОБЫ ИХ ПОЛУЧЕНИЯ

Предложены оптически активные адсорбенты на основе сетчатых полимеризованных производных дикарбоновых кислот, диаминов или диолов, которые химически связаны с носителем. Производные этих соединений можно полимеризовать радикальной полимеризацией или через гидросилирование в присутствии твердого носителя. Оптически активные адсорбенты эффективны для хроматографического разделения рацемических смесей энантиомеров. 9 с. и 13 з.п. ф-лы.

СПОСОБ РАЗДЕЛЕНИЯ ХИРАЛЬНЫХ СУЛЬФОКСИДОВ С ПОМОЩЬЮ ЭНАНТИОСЕЛЕКТИВНОЙ ХРОМАТОГРАФИИ

Изобретение относится к области химии, а именно к разделению хиральных сульфоксидов, которые широко применяются в синтезе хиральных органических соединений. Описан гомохиральный металлорганический микропористый полимерный материал, способный к энантиоселективной сорбции сульфоксидов, и разработана методика хроматографического разделения хиральных сульфоксидов на колонке, наполненной этим материалом в качестве хиральной неподвижной фазы. Технический результат - возможность препаративного хроматографического разделения хиральных сульфоксидов. 2 табл., 4 ил.

Verfahren zur Auftrennung racemischer Mischungen

Verfahren zur Auftrennung einer racemischen Mischung, umfassend die Entwicklung einer dichteren molekularen Prägung auf Silika mit einem gewünschten Enantiomer durch das Sol/Gel-Protokoll, wobei das Sol/Gel-Protokoll die hydrolytisch kontrollierte Polymerisierung einer Silikaquelle als Monomer und Aminoalkylsilan als einem funktionalen Monomer in Gegenwart des gewünschten Enantiomers, das Mit-Schutzgruppen-versehen (Capping), der Oberflächen OH-Gruppen und die Desorption des verkapselten Enantiomers aus dem Silika umfasst, zur selektiven Absorption von 3 bis 12% des Enantiomers aus der racemischen Mischung, wobei die Auftrennung mit hoher optischer Reinheit bewirkt wird.

MEANS FOR THE SEPARATION OF OPTICAL ISOMERS

NOVEL CYCLODEXTRIN DERIVATIVES, PREPARATION METHOD THEREOF AND APPLICATIONS OF SAME

Process for preparing purified s-bel and r-bel and compositions thereof

PROCESS FOR THE SEPARATION OF RACEMIC MIXTURES

PACKING MATERIAL FOR LIQUID CHROMATOGRAPHY

A packing material for liquid chromatography, which comprises an optically active compound having the formula: (I) wherein Ar is a phenyl group, an a-naphthyl group or a .beta.-naphthyl group, R1 is an alkyl group having from 1 to 4 carbon atoms, a phenyl group or a 3,5-dinitrophenyl group, R2 is an alkyl group having from 1 to 4 carbon atoms, * indicates an asymmetric carbon atom and n is an integer of 2 or 3, as a stationary phase.

CHIRAL POROUS COORDINATION POLYMER, ITS DESOLVATED FORM AND A METHOD FOR THE CHROMATOGRAPHIC SEPARATION OF THE OPTICALLY ACTIVE COMPOUNDS ISOMERS

Preparation method 5-(4- and application of -1- R-Cu(I) chirality)-1H- (C) material MOF based on imidazolo-(phenyl)-substituted tetrazole (fentrazole)

Beta-cyclodextrin functionalized chiral stationary phase, preparation and application thereof

Cyclodextrin derivatives with carbinol groups, are used in solutions, gels, supports or preparations for pharmaceutical, cosmetic, agrochemical or food-related applications

... 1. Nouveaux dérivés de cyclodextrines caractérisés par le fait qu'au moins l'un des atomes d'hydrogène portés par les atomes de carbone en position 3, et/ou 5 et/ou 6 des unités glucopyranosidiques (ou glucose) entrant dans la constitution de la cyclodextrine, est substitué par un groupement carbinol c'est-à-dire par un carbone tétravalent portant une fonction hydroxyle secondaire, les dérivés conformes à l'invention répondant à la formule générale : dans laquelle - n est égal à 0, 1 ou 2, et dans laquelle au moins des l'un des radical R3 représente un groupement carbinol R1-C(OH)-R2 ...

Filler used for separating optical isomers and process for separating optical isomers with the filler

The objectives of this invention are to provide a filler for separating optical isomers, capable of efficiently carrying out optical resolution of optical isomer mixtures, and to provide a process for separating optical isomers by a simulated moving bed chromatography utilizing the filler. This invention provides a filler made of a carrier supporting a specific amount of an optically active high molecular weight compound. Separation by the simulated moving bed chromatography using the filler is carried out under the condition that the capacity factors have specific values.

СОРБЕНТ ДЛЯ ГАЗОХРОМАТОГРАФИЧЕСКОГО РАЗДЕЛЕНИЯ ЭНАНТИОМЕРОВ (ВАРИАНТЫ) И СПОСОБ ЕГО ИСПОЛЬЗОВАНИЯ

Изобретение относится к созданию неподвижных фаз для разделения энантиомеров методом газовой хроматографии и может быть использовано в химической и фармацевтической промышленности для анализа энантиомеров. Предложен сорбент для газохроматографического разделения энантиомеров, состоящий из инертного носителя марки Chromaton NAW и нанесенного оптически активного соединения. В качестве оптически активного соединения используют супрамолекулярную структуру урацила или 5-гидрокси-6-метилурацила. Изобретение позволяет осуществить эффективное разделение энантиомеров при температуре 70-80°С. 2 н.п. ф-лы, 1 табл, 2 пр.

СОРБЕНТ ДЛЯ ХРОМАТОГРАФИИ ОПТИЧЕСКИХ ИЗОМЕРОВ И СПОСОБ ЕГО ПОЛУЧЕНИЯ

Изобретение относится к сорбентам для хроматографии. Предложен сорбент на основе силикагеля с привитым через спейсер гибридным хиральным селектором, полученный путем модифицирования силикагеля эпоксигруппами, прививки эремомицина и хирального полианилина. Сорбент проявляет повышенную энантиоселективность к различным соединениям. 2 н. и 1 з.п. ф-лы, 2 табл., 9 ил.

MODIFIED SILICAGEL AS CARRIER FOR LIGAND-EXCHANGE CHROMATOGRAPHY AND ITS PREPARATION METHOD

Модифицированный кремнезем в качествеСОРбЕНТА для лигАНдООбМЕННОй XPO-МАТОгРАфии

PARTING AGENT FOR OPTICAL ISOMERS AND PROCEDURE FOR ITS PRODUCTION

MANUFACTURING PROCESS OF A FILLER FOR THE SEPARATION OF AN OPTICAL ISOMER AND APPLICATION IN THE CHROMATOGRAPHY

USE of NON--INDIVIDUAL SORBENTIEN FOR ßSIMULATED MOVING BEDß SEPARATION PROCESS

NICHTSYMMETRI COMPOUND

Chiral drug resolution method based on porous silica gel modified by chemical bonds

Abstract The invention discloses a chiral drug resolution method based on porous silica gel modified by chemical bonds, which uses porous modified silica gel modified by chemical bonds to resolve chiral drugs,The preparation method of porous modified silica gel comprises the following steps: A, taking porous silica gel and silanizing reagent for later use; B, chemically modifying the porous silica gel to obtain modified silica gel containing terminal amino groups; C, taking chiral drug-related protein to be resolved, and carrying out enzymolysis at 22-38 DEG C to obtain a polypeptide combination library; And d, chemically modifying and bonding the polypeptide combination library obtained in the step c on the surface of the modified silica gel obtained in the step b to obtain porous modified silica gel for chiral drug resolution. The combined chiral recognition library on the surface of the porous modified silica gel prepared by the invention can be used for the resolution and analysis of ...

INORGANIC MESOPOROUS SUBSTANCE HAVING CHIRAL TWISTED STRUCTURE AND PROCESS FOR PRODUCING THE SAME

ENANTIOMERIC SILANES, MODIFIED PACKING MATERIAL, AND USE THEREOF

A compound of formula I (I), wherein R1 is C1-C4alkyl, phenyl or benzyl, R2 is C1-C4alkyl, phenyl or benzyl, a is 0,1 or 2, R3 is linear or branched unsubstituted or OH-substituted C1-C12alkylene or is phenylene, R5 is the divalent radical, diminished by the -CO-O group, of a lactone having a total of 4 to 7 ring members and containing at least one chiral carbon atom and corresponding predominantly to an optically active enantiomeric form, R4 is a direct bond, C1-C4alkyl-CH or CF3-CH, and Y is phenyl, naphthyl, fluorenyl or anthryl, each unsubstituted or substituted by C1-C12alkyl, C1-C12alkoxy, halogen, -CN or -NO2. These compounds are suitable for the preparation of stationary phases for the chromatographic separation of chiral compounds.

CHIRAL ADSORBENTS AND PREPARATION THEREOF AS WELL AS COMPOUNDS ON WHICH THE ADSORBENTS ARE BASED AND PREPARATION OF THESE COMPOUNDS

Optically active absorbents based on network polymerised derivatives of dicarboxylic acids, diamines or diols which are chemically bonded to a carrier. The derivatives can be polymerised by radical polymerisation or through hydrosilylation in the presence of a solid carrier. The optically active absorbents are usable for chromatografic separation of racemic mixtures of enantiomers.

Lipase bonded stationary phase and preparation method and use thereof

The invention discloses a lipase bonded stationary phase and a preparation method and use thereof in the field of chromatographic stationary phase preparation. The stationary phase is obtained by directly bonding a spherical silica gel filler with an aldehyde group on the surface and the amino group of the lipase by an imine bond, and then reducing the imine into the amino group. The stationary phase has very high chiral recognition capability, not only can be used as the filler of a chiral chromatographic column, but also can be used as the filler of an online liquid-phase chromatographic reactor, and has good chemical stability, therefore, quite high activity can be kept within three months; furthermore, the preparation cost is relatively low, and the application prospect is good no matter in filling analytical columns or preparative columns.

Oligopeptide-like chiral selector and preparation method thereof

The invention provides an oligopeptide-like chiral selector and a preparation method thereof. The oligopeptide-like chiral selector is prepared by connecting a plurality of same or different amine compounds (HNR2R3)(chiral amine generally) by using a halogenated carboxylic acid or acylation reagent (XCO-R1-Y); and a general molecular formula of the oligopeptide-like chiral selector can be represented as [R2'R3'N(-CO-R1'-)NR2''(-CO-R1''-)NR2'''...(-CO-R1...-)NR2...R3...], wherein a connecting part R1 on a main chain as well as a substituent group R2 and a substituent group R3 on a side chain can be chiral or achiral, but at least one of the connecting part R1, the substituent group R2 and the substituent group R3 is chiral. Due to a flexible primary structure and a flexible secondary structure, the chain oligopeptide-like compound can be simply derived and then is coated or bonded onto the surface of a carrier to form an effective chiral stationary phase. The kind of chiral selector has ...

Method for preparing carbon nanotube-protein stationary phase

Process for producing hydrogel with optical activity

The invention relates to a method for preparing a hydrogel with optical activity, which pertains to the field of polymer material. The method comprises the following steps: firstly, an N-propargyl amide monomer with double bond is synthesized, the monomer is then copolymerized with N-propargyl amide, N-propargyl carbamide or N-propargyl sulfonamide monomer with chiral substituent to synthesize a copolymer with stable helical structure and rather high optical activity; then the copolymer obtained is dissolved in a chloroform solvent, propyl acrylamide (NIPAm) and N, N`- methylene bisacrylamide(Bis) are dissolved in chloroform, the NIPAm dissolved is added into the copolymer solution and heated up to 45 DEG C to 90 DEG C under the protection of nitrogen; an evocating agent AIBN is added under the protection of nitrogen, reaction lasts for 30min to 24h and then a yellow clear gel is obtained. The gel is put into the chloroform solvent to be soaked and cleaned for a plurality of times, dried ...

Silver functionalized silicon base chiral chromatographic stationary phase and its preparation method

CHIRAL INORGANIC-ORGANIC COMPOSITE POROUS MATERIAL USED AS SELECTIVE CHIRAL CATALYST OR ISOMER MIXTURE SEPARATING MATERIAL AND METHOD FOR PREPARING THE SAME

PURPOSE: To provide a chiral inorganic-organic composite porous material, a method for preparing the chiral inorganic-organic composite porous material, and a method for using the chiral inorganic-organic composite porous material as a selective chiral catalyst or an isomer separating material. CONSTITUTION: In a porous material comprising electric charge balancing cations, a chiral inorganic-organic composite porous material is characterized in that cationic chiral organic molecules exist as electric charge balancing cations. A method for preparing the chiral inorganic-organic composite porous material comprises a step of ion exchanging the electric charge balancing cations of a porous material comprising electric charge balancing cations with cationic chiral organic molecules, wherein the method comprises: a step(a) of ion exchanging the electric charge balancing cations of the porous material comprising electric charge balancing cations with transition metal cations; and a step(b) of ...

ORGANIC-INORGANIC HYBRID CHIRAL SORBENT AND PROCESS FOR THE PREPARATION THEREOF

PROCESS FOR PRODUCING PACKING FOR RESOLVING OPTICAL ISOMERS

USE OF NON-PARTICULATE SORBENTS FOR SIMULATED MOVING BED SEPARATING METHODS

The invention concerns the use of non-particulate sorbents for preparative separating methods, in particular for separating methods operating according to the simulated moving bed (SMB) principle. In particular, the invention concerns the use of monolithic sorbents based on porous shaped bodies which comprise interconnected macropores and mesopores in the macropore walls, the diameter of the macropores having a median of more than 0.1 'mu'm, and the diameter of the mesopores having a median of between 2 and 100 nm. The invention also concerns the use of sorbents consisting of modified polyamide containing separation effectors that are bonded to the polyamide amino groups via a bridge group for separation methods carried out according to the simulated moving bed (SMB) principle.

MULTIPLE STATIONARY PHASE MATRIX AND USES THEREOF

The present invention generally provides a separation matrix comprising at least two stationary phases and a stationary phase comprising at least one chiral modality and at least one achiral modality. Also provided are methods of using the separation matrix or the stationary phase to separate enantiomers of one or more chiral molecules. 1. A stationary phase comprising at least one chiral modality and at least one achiral modality.2. The stationary phase of claim 1 , wherein the chiral modality is chosen from a macrocyclic glycopeptide claim 1 , a cyclodextrin claim 1 , a polysaccharide polymer claim 1 , a small molecule claim 1 , and a protein.3. The stationary phase of claim 1 , wherein the achiral modality is chosen from alkyl claim 1 , alkenyl claim 1 , alkynyl claim 1 , aryl claim 1 , alkylaryl claim 1 , alkylamide claim 1 , alkylamino claim 1 , alkyldiol claim 1 , alkylcarboxy claim 1 , alkylsulfonic claim 1 , amide claim 1 , amine claim 1 , cyano claim 1 , diol claim 1 , carboxy claim 1 , and sulfonic.4. The stationary phase of claim 1 , wherein the stationary phase is affixed to a solid support.5. The stationary phase of claim 4 , wherein the solid support is chosen from silica claim 4 , silica gel claim 4 , alumina claim 4 , glass claim 4 , metal claim 4 , a polymer claim 4 , a co-polymer claim 4 , and combinations thereof.6. The stationary phase of claim 5 , wherein the solid support comprises a plurality of particles claim 5 , the plurality of particles having an average particle diameter from about 0.5 micron to about 15 microns and an average pore size from about 25 angstroms to about 500 angstroms.7. The stationary phase of claim 1 , wherein the stationary phase is used in a technique chosen from high performance liquid chromatography claim 1 , ultra high performance liquid chromatography claim 1 , supercritical fluid chromatography claim 1 , simulated moving bed chromatography claim 1 , gas chromatography claim 1 , ion chromatography claim 1 , counter ...

Filler used for separating optical isomers and process for separating optical isomers with the filler

The objectives of this invention are to provide a filler for separating optical isomers, capable of efficiently carrying out optical resolution of optical isomer mixtures, and to provide a process for separating optical isomers by a simulated moving bed chromatography utilizing the filler. This invention provides a filler made of a carrier supporting a specific amount of an optically active high molecular weight compound. Separation by the simulated moving bed chromatography using the filler is carried out under the condition that the capacity factors have specific values.

Method of adsorbing subtances

Process for the separation of racemic mixtures

PROCEDURE FOR THE PRODUCTION OF A FILLER FOR THE SEPARATION OF ENANTIOMERI ISOMERS

CHIRALE ADSORBENTIEN AND THEIR PRODUCTION, AS WELL AS CONNECTIONS ON WHICH THE ADSORBETNTIEN ITSELF REASONS AND THE PRODUCTION OF THESE CONNECTIONS

NEW PARTING AGENT FOR THE SEPARATION OF AN OPTICAL ISOMER AND MANUFACTURING PROCESS FOR IT

REVERSIBLE BIO GEL FOR THE TREATMENT AND SEPARATION FROM SINGLE WALL CARBON NANO-TUBES

Packing material for separation of optical isomer and method of separating optical isomer with the same

新型光学异构体分离用分离剂及其制备方法

... 一种新型光学异构体分离用分离剂的制备方法，其特征在于：该方法包括：用溶剂把光学活性高分子化合物和分子量为1000或1000以下的具有不对称结构的化合物、负载在载体上的工序；除去溶剂的工序；洗涤除去分子量为1000或1000以下的具有不对称结构的化合物工序。 ...

A temperature response of the β-cyclodextrin silica gel stationary phase and its preparation method

Ginsenoside Rg1-phenyl isocyanate chiral stationary phase filler and preparation method thereof

Application of single-hand spiral tubular silica as gas chromatography stationary phase

ORGANIC-INORGANIC HYBRID CHIRAL SORBENT AND PROCESS FOR THE PREPARATION THEREOF

The present invention provides an organic-inorganic hybrid chiral sorbent for chiral resolution of various racemic compounds viz. racemic mandelic acid, 2- phenyl propionic acid, diethyl tartrate, 2,2'-dihydroxy-1,1 '-binaphthalene (BINOL) and cyano chromene oxide with excellent chiral separation (enantiomeric excess, 99 %) in case of mandelic acid under medium pressure column chromatography. These optically pure enantiomers find applications as intermediates in pharmaceutical industries.

IMPROVED ANTIBODY BINDING AFFINITY LIGANDS

The present invention discloses particularly useful ligands covalently bound to a solid support material, such as a polymer matrix, and uses thereof in the purification and/or isolation of biomolecules, such as proteins, in particular antibodies, such as monoclonal antibodies. The ligands include compounds of the formula (3,5-di-tert-butyl-4-hydroxy-benzoyl)-Arg-Arg-.

SEPARATORY AGENT FOR OPTICAL ISOMER

A separatory agent for optical isomers which contains a polysaccharide derivative as an active ingredient, wherein the polysaccharide derivative serving as an active ingredient is one formed by replacing at least part of the hydrogen atoms of the hydroxy groups and amino groups of a polysaccharide with at least one member selected between a benzoyl group having a specific alkyl group and a carbamoyl group in which one hydrogen atom has been replaced with an aromatic group having a specific alkyl group. It is a novel separatory agent for optical isomers which has the high ability to asymmetrically identify the target compound to be analyzed and isolated.

АДСОРБЕНТ ДЛЯ ГАЗОХРОМАТОГРАФИЧЕСКОГО РАЗДЕЛЕНИЯ ЭНАНТИОМЕРОВ

Изобретение относится к аналитической химии, в частности к созданию адсорбентов для разделения энантиомеров методом газовой хроматографии. Адсорбент состоит из инертного носителя Chromaton NAW и оптически активного соединения, представляющего собой супрамолекулярную структуру меламина, нанесенную на носитель в количестве 1% от массы носителя. Технический результат: повышение селективности по отношению к энантиомерам, сокращение продолжительности процесса разделения. 1 табл.

СПОСОБ ПОЛУЧЕНИЯ ТОНКОСЛОЙНЫХ ХИРАЛЬНЫХ ПЛАСТИН ДЛЯ ПЛАНАРНОЙ ХРОМАТОГРАФИИ СТЕРЕОИЗОМЕРОВ И ИХ РАЦЕМИЧЕСКИХ СМЕСЕЙ

... 1. Способ получения тонкослойных хиральных пластин для планарной хроматографии стереоизомеров и их рацемических смесей, включающий связывание хирального агента с поверхностью адсорбента, закрепленного на ТСХ-пластине с помощью связующего, отличающийся тем, что в качестве хирального агента используют эремомицин, нековалентно связанный с сорбентом методом импрегнирования, имеющий следующую структурную формулу2. Способ получения по п.1, отличающийся тем, что в качестве адсорбента используют силикагель с силиказолем в качестве связующего с максимально гидроксилированной поверхностью (силанольное число α-=4,6÷4,9 ОН-групп/нм), активированный водным раствором 0,2М НSOпри температуре 25°С в течение 20-60 мин. и промытый деминерализованной водой до рН 5÷7.3. Способ получения по п.1, отличающийся тем, что нековалентное связывание гликопептидного антибиотика эремомицина с поверхностью адсорбента производят методом импрегнирования в щелочном водном растворе эремомицина при рН 8,0÷10,0, при этом процесс ...

СОРБЕНТ ДЛЯ РАЗДЕЛЕНИЯ ОПТИЧЕСКИХ ИЗОМЕРОВ ВЕЩЕСТВ И ИХ АНАЛИЗА В БИОЛОГИЧЕСКИХ ЖИДКОСТЯХ МЕТОДОМ ВЭЖХ И СПОСОБ ЕГО ПОЛУЧЕНИЯ

... 1. Сорбент для разделения оптических изомеров веществ и для их анализа в биологических жидкостях методом ВЭЖХ, содержащий силикагель, модифицированный гликопептидным антибиотиком эремомицином, отличающийся тем, что он дополнительно модифицирован бычьим сывороточным альбумином.2. Способ получения сорбента для хроматографии оптических изомеров, включающий модифицирование поверхности силикагеля эпоксигруппами, иммобилизацию эремомицина на поверхности силикагеля с привитыми эпоксигруппами, промывку, сушку, отличающийся тем, что силикагель, модифицированный эремомицином, после сушки подвергают взаимодействию с бычьим сывороточным альбумином, глутаровым альдегидом и тетрагидроборатом натрия.

СОРБЕНТ ДЛЯ ГАЗОХРОМАТОГРАФИЧЕСКОГО РАЗДЕЛЕНИЯ ЭНАНТИОМЕРОВ (ВАРИАНТЫ) И СПОСОБ ЕГО ИСПОЛЬЗОВАНИЯ

... 1. Сорбент для газохроматографического разделения энантиомеров, состоящий из инертного носителя марки Chromaton NAW и нанесенного оптически активного соединения, отличающийся тем, что в качестве оптически активного соединения используют супрамолекулярную структуру урацила, взятую в количестве 10% от массы инертного носителя.2. Сорбент для газохроматографического разделения энантиомеров, состоящий из инертного носителя марки Chromaton NAW и нанесенного оптически активного соединения, отличающийся тем, что в качестве оптически активного соединения используют супрамолекулярную структуру 5-гидрокси-6-метилурацила, взятую в количестве 1-10% от массы инертного носителя.3. Способ применения сорбента по п. 1 и 2, отличающийся тем, что разделение энантиомеров на сорбенте проводят при 70-80°C.

TRENNBARES MITTEL FÜR OPTISCHEN ISOMER

Process for the separation of racemic mixtures

The present invention relates to a process for the separation of racemic mixtures comprising development of a denser molecular imprint on silica with a desired enantiomer by sol-gel-protocol comprising hydrolytic control polymerization of a silica source as the monomer and amino alkylsilane as a functional monomer in the presence of the desired enantiomer, capping of surface OH groups and desorption of ancapsulated enantiomer from the silica.

NOVEL CHIRAL SELECTORS AND STATIONARY PHASES FOR SEPARATING ENANTIOMER MIXTURES

The invention relates to the use of a-unsubstituted ß-amino acid derivatives having the structure (I), as chiral selectors in methods for separating mixtures of substances, preferably of mixtures of chiral substances, more preferred of enantiomer mixtures, especially of enantiomers of substances that are selected from the group containing ß-amino acids and the derivatives thereof, a-amino acids and a-hydroxy acids, wherein n = 1-5, preferably n = 1 or n = 2, R1 = (C1-C4) alkyl, (C6-C10) aryl, (C7-C13) aralkyl, (C7-C10) heteroaralkyl, pyridyl, hydroxymethyl, CH(OH)CH3, CH2CONH2, CH2COOH, (CH2)2CONH2, (CH2)2COOH, (CH2)4NH2, (CH2)2SCH3 or (CH2)3NHC(NH)NH2 and R2 = 3,5-dinitrobenzoyl or naphthyl, if X is a linker for the covalent bond to a carrier material, or R2 = CH2CHR3R4, wherein R3 = H or OH and R4 = (C1-C20) alkyl, (C6-C10) aryl or (C7-C13) aralkyl, if X = OH, and to the use of said selectors for producing chiral stationary phases. The invention further relates to the chiral stationary ...

NOVEL CHIRAL SELECTORS AND STATIONARY PHASES FOR SEPARATING ENANTIOMER MIXTURES

The invention relates to the use of .alpha.-unsubstituted .beta.-amino acid derivatives having the structure (I), as chiral selectors in methods for separating mixtures of substances, preferably of mixtures of chiral substances, more preferred of enantiomer mixtures, especially of enantiomers of substances that are selected from the group containing 0-amino acids and the derivatives thereof, .alpha.-amino acids and .alpha.-hydroxy acids, wherein n = 1-5, preferably n = 1 or n = 2, R1 =(C1-C4) alkyl, (C6-C10) aryl, (C7-C13) aralkyl, (C1-C10) heteroaralkyl, pyridyl, hydroxymethyl, CH(OH)CH3, CH2CONH2, CH2COOH, (CH2)2CONH2, (CH2)2COOH, (CH2)4NH2, (CH2)2SCH3 or (CH2)3NHC(NH)NH2 and R2 = 3,5-dinitrobenzoyl or naphthyl, if X is a linker for the covalent bond to a carrier material, or R2 = CH2CHR3R4, wherein R3 = H or OH and R4 =(C1-C20) alkyl, (C6-C10) aryl or (C7-C13) aralkyl, if X = OH, and to the use of said selectors for producing chiral stationary phases. The invention further relates to ...

Preparation method and application of omega-diamine derivatization beta-cyclodextrin bonded SBA-15 chiral stationary phase

The invention relates to a preparation method and an application of an omega-diamine derivatization beta-cyclodextrin bonded SBA-15 chiral stationary phase. The preparation method comprises steps as follows: 6-p-tosylation beta-cyclodextrin and omega-diamine are taken as raw materials, the omega-diamine is a solvent, and 6-(omega-diamine)-beta-cyclodextrin ligand is prepared through heating; the 6-(omega-diamine)-beta-cyclodextrin ligand and 3-isocyanato propyl siloxane react to prepare siloxane containing the 6-(omega-diamine)-beta-cyclodextrin ligand; anhydrous N,N-dimethyl formamide is taken as a solvent and heated under the protection of nitrogen, so that the siloxane containing the 6-(omega-diamine)-beta-cyclodextrin ligand reacts with a silanol hydroxyl group on the surface of SBA-15 silica gel, and a coarse product is obtained; and redundant ligand and impurities in holes are cleared, and a final product is obtained. The preparation method is simple, convenient, lower in cost, wide ...

Side base band with the double-chiral carbon atom of the phenylacetylene derivative and its preparation and application method

Method for splitting 3-acetamido-3-phenyl-methyl propionate enantiomer

NEW DERIVATIVES OF CYCLODEXTRINS, THEIR METHOD OF PREPARATION AND LEURSAPPLICATIONS

SEPARATING AGENT FOR OPTICAL ISOMER

Disclosed is a separating agent for optical isomers which exhibits stable separating performance while having high optical resolving power and sufficient solvent resistance at the same time. Specifically disclosed is a separating agent for optical isomers wherein an optically active polymer compound such as cellulose or amylose is supported by a carrier such as a silica gel through chemical bonding. The separating agent for optical isomers has a specific surface area of 10-150 m 2/g and an average particle diameter of 1-100 μm. © KIPO & WIPO 2007 ...

CHIRAL STATIONARY PHASES BASED ON XANTHONE DERIVATIVES

The present invention relates to the development of new chiral stationary phases (CSPs), named chiral xanthone stationary phases (CXSPs), and to the use thereof for the enantiomeric resolution by high-performance liquid chromatography (HPLC). More specifically, it relates to the invention of new xantone-derived chiral stationary phases. The invention relates to the preparation of this type of phases by linking a chiral xanthone derivative (chiral selector) to a spacer that enables subsequently anchoring the chiral selector to a chromatographic support. This new CSP has typical interactions of the Pirkle CSP type and thus possesses all the attributes necessary to achieve chiral recognition and resolution of a variety of enantiomers belonging to various classes of compounds, in various elution modes which involve normal, polar organic and reverse phases.

CHIRAL SELECTORS FOR SEPARATING ENANTIOMERS OF A COMPOUND

The invention relates to the use of compounds as new chiral selectors for separating the optical or enantiomeric isomers of a compound, wherein the chiral selector comprises at least one compound of formula (I): and at least one metal ion, for example Cu2+, Ni2+, Zn2+, Cd2+ or Co2+.

OPTICAL ISOMER SEPARATION PACKING FOR SIMULATED MOVING BED CHROMATOGRAPHY

A packing that is suitable for fractionation by simulated moving bed chromatography, ensuring high productivity. In particular, an optical isomer separation packing for simulated moving bed chromatography wherein a polysaccharide derivative is borne on a porous support, characterized in that the TS coefficient defined by the following formula (I), obtained with the use of an optical isomer separation column for simulated moving bed chromatography wherein the packing is charged in a column pipe according to slurry filling technique, is in the range of 0.55 to 1.20. TS coefficient = [Vc - [t(TS) - t(blank)] Χ FR] / [t(TS) - t(blank)] Χ FR (I) wherein Vc represents column volume (cm3); FR represents flow rate (ml/min); t(TS) represents elution time (min) of tetrakis(trimethylsilyl)silane (=TS); and t(blank) represents TS elution time exhibited in column non-connection condition.

Cationic Oligomer of a Saccharide for Resolving Enantiomers and Asymmetric Synthesis

A cationic oligomer of a saccharide, wherein the saccharide is functionalized by a cationic group, for example an ammonium, phosphonium, imidazolium, or pyridinium group. In a preferred embodiment, the cationic oligomer of a saccharide is a cationic cyclodextrin. There is also provided a use of the cationic oligomer of a saccharide as a chiral agent for resolving enantiomers by a chromatographic method or an asymmetric synthesis.

Adsorbent

A method of adsorbing a substance such as an optically active substance, comprising contacting a solution of the substance in a solvent with an adsorbent, consisting of a cross-linked, polymer comprising an optically active, synthetic·poly(amino acid)represent by the general formula (I):



wherein R stands for an organic group, R' stands for hydrogen or an alkyl group and n is an integer of at least 5, which may be protected at a terminal amino group and a carrier polymer supporting said synthetic poly(amino acid) thereon, said carrier polymer having affinity for said solvent.

The adsorbing is especially useful for optical resolution of racemic mixtures.

Separation agent comprising polysaccharide carbamate

A chemical substance is separated by treating a mixture containing the same with a carbamate derivative of a polysaccharide selected from the group consisting of cellulose, amylose, chitosan, xylan, dextran and inulin, except for an aromatic carbamate of cellulose.

СОРБЕНТ ДЛЯ РАЗДЕЛЕНИЯ ОПТИЧЕСКИХ ИЗОМЕРОВ ВЕЩЕСТВ И ИХ АНАЛИЗА В БИОЛОГИЧЕСКИХ ЖИДКОСТЯХ МЕТОДОМ ВЭЖХ И СПОСОБ ЕГО ПОЛУЧЕНИЯ

Изобретение относится к сорбентам для высокоэффективной жидкостной хроматографии (ВЭЖХ), в частности к получению химически модифицированных сорбентов. Предложен сорбент на основе силикагеля с привитым через спейсер гибридным хиральным селектором. Сорбент получен путем модифицирования силикагеля эпоксигруппами, прививки эремомицина и бычьего сывороточного альбумина. Сорбент проявляет повышенную энантиоселективность к различным соединениям, в том числе при их анализе в биологических жидкостях. 2 н.п. ф-лы, 4 ил., 2 табл.

СПОСОБ ПОЛУЧЕНИЯ ТОНКОСЛОЙНЫХ ХИРАЛЬНЫХ ПЛАСТИН ДЛЯ ПЛАНАРНОЙ ХРОМАТОГРАФИИ СТЕРЕОИЗОМЕРОВ И ИХ РАЦЕМИЧЕСКИХ СМЕСЕЙ

Изобретение относится к способу получения тонкослойных хиральных пластин для планарной хроматографии стереоизомеров и их рацемических смесей, который включает нековалентное связывание гликопептидного антибиотика эремомицина с кремнезёмным адсорбентом с силикагелевым связующим методом импрегнирования в щелочном водном растворе при рН 8,0÷10,0 при комнатной температуре в одну стадию. 1 з.п. ф-лы, 3 табл., 5 пр.

СОРБЕНТ И СПОСОБ ЕГО ПОЛУЧЕНИЯ

Изобретение относится к области разделения рацематов оптически активных соединений путем хроматографии. Предложен сорбент на основе производных циклодекстринов, ковалентно иммобилизованных на носителях, при этом в качестве хиральных селекторов используют пер-6-аминопроизводные α-,β- или γ -циклодекстрина либо их ацетилированные аналоги. Предложен также способ получения сорбента. Изобретение позволяет расширить круг разделяемых веществ. 2 с. и 4 з.п. ф-лы, 1 табл.

СОРБЕНТ ДЛЯ ХРОМАТОГРАФИИ ОПТИЧЕСКИХ ИЗОМЕРОВ И СПОСОБ ЕГО ПОЛУЧЕНИЯ

... 1. Сорбент для хроматографии оптических изомеров, содержащий силикагель, модифицированный гликопептидным антибиотиком - эремомицином, отличающийся тем, что он дополнительно модифицирован хиральным полианилином. 2. Способ получения сорбента для хроматографии оптических изомеров, включающий модифицирование поверхности силикагеля эпоксигруппами, иммобилизацию эремомицина на поверхности силикагеля с привитыми эпоксигруппами, промывку, сушку, отличающийся тем, что силикагель, модифицированный эремомицином, подвергают взаимодействию с анилином в присутствии фермента лакказы и энантиомера сульфокамфорной кислоты. 3. Способ по п.2, отличающийся тем, что для модифицирования поверхности силикагеля эпоксигруппами используют обработку 3-глицидоксипропилтриэтоксисиланом.

Способ получения энантиоселективного сорбента

Изобретение относится к аналитической химии, в частности к способу создания энантиоселективных сорбентов. Cпособ заключатся в модифицировании графитированной термической сажи Carboblack С или инертного носителя Inerton NAW супрамолекулярной структурой циануровой кислоты. Модифицирование проводят при 30-60°C в водно-спиртовом растворе при механическом размешивании с интенсивностью 500 rpm. Полученный сорбент подвергают сушке. Изобретение обеспечивает получение сорбента с высокой селективностью при разделении 2-хлорбутана, 2 бромбутана, 2-бутанола и 2 пентанола. 8 пр.

Chiral chromatographic stationary phase of polymer-coated silica and preparation method thereof

The invention discloses a chiral chromatographic stationary phase of polymer-coated silica and a preparation method thereof, belonging to the technical field of the material chemistry. The chromatographic stationary phase has the structure that a grafted azide group is grafted on the surface of a silica microsphere with a cyclodextrin polymer layer. The preparation method adopted by the chiral chromatographic stationary phase of the polymer-coated silica comprises the steps of coating a polymer layer containing alkynyl on the surface of the silica microsphere by adopting an atom transfer redical polymerization, taking methacrylic acid-methyl-butyne alcohol ester as an monomer, taking divinylbenzene as a cross-linking agent, and then clicking chemically grafted cyclodextrin containing the azide group to obtain the chiral chromatographic stationary phase. The chiral chromatographic stationary phase of the polymer-coated silica has the advantages that the polymer coating layer in the stationary ...

(R)- BIBIBIL derivative CSP filler as well as preparation method and application thereof

Polypeptide supramolecular chiral filler for high performance liquid chromatography as well as preparation method and application thereof

A β - cyclodextrin-modified gold nanoparticle/silica gel chiral stationary phase and its preparation method

ORGANIC-INORGANIC HYBRID CHIRAL SORBENT AND PROCESS FOR THE PREPARATION THEREOF

The present invention provides an organic-inorganic hybrid chiral sorbent for chiral resolution of various racemic compounds viz. racemic mandelic acid, 2-phenyl propionic acid, diethyl tartrate, 2,2'-dihydroxy-1,1 '-binaphthalene (BINOL) and cyano chromene oxide with excellent chiral separation (enantiomeric excess, 99 %) in case of mandelic acid under medium pressure column chromatography. These optically pure enantiomers find applications as intermediates in pharmaceutical industries. © KIPO & WIPO 2009 ...

PROCESS FOR PRODUCING PACKING FOR RESOLVING OPTICAL ISOMERS

It is intended to provide a production process whereby a polysaccharide derivative can be uniformly loaded on a porous support. Namely, a production process which comprises contacting a porous support with a polysaccharide derivative solution by stirring in a stirring device to thereby load an optically active polymer compound on the porous support. In the above process which aims at producing a packing for resolving optical isomers, a vertical twin-screw stirring device is employed as the stirring device and the polysaccharide derivative is loaded on the porous support to give a loading dose of 23% by mass or more.

AGENT FOR SEPARATING OPTICAL ISOMERS

An agent for separating optical isomers, which comprises, as the effective component thereof, a polysaccharide derivative wherein at least a part of hydrogen atoms of the hydroxyl groups of the polysaccharide is substituted with at least one of the groups represented by the general formulae (I) and (II): [wherein R represents a substituted or unsubstituted aromatic group or an aliphatic group of a linear chain, a branched chain or a ring form]. The agent for separating optical isomers exhibits improved separating performance.

Separating agent for optical isomers

To provide a novel separating agent for optical isomers based on a polymer having an optically active moiety, provided is a separating agent for optical isomers formed of: a helical polymer obtained by using an aromatic isonitrile as a monomer having an amide group in which an optically active amino acid is amide-bonded to an aromatic ring; and a carrier for carrying the helical polymer by being chemically bonded to an end of the helical polymer.

Removable final scrubber tube

A final scrubber in the inert carrier gas flow path of an elemental analyzer includes a manifold with valves for selectively bypassing a quick disconnect final scrubber housing that includes a filter tube and sealed gas fittings. The housing includes alignment members and a latch for positioning and locking the housing onto and in sealed engagement with the instrument's manifold. A switch detects the presence of the housing, and a control circuit controls valves to direct the inert gas flow though the filter tube or bypass the filter tube when the housing is removed. With this system, the final scrubber can be removed and replaced quickly without the use of tools while the carrier gas continues to flow though the furnace without interruption. Also, the valves can be closed to allow for segmented leak detection of the instruments gas flow path.

High Stability Diionic Liquid Salts

The present invention relates to diionic liquid salts of dicationic or dianionic molecules, as well as solvents comprising diionic liquids and the use of diionic liquids as the stationary phase in a gas chromatographic column.

Methods and devices for nucleic acid purification

The invention provides pipette tip columns and automated methods for the purification of nucleic acids such as plasmids from unclarified cell lysates containing cell debris as well as methods for making and using such columns. The columns typically include a bed of medium positioned in the pipette tip column, above a bottom frit and with an optional top frit.

Molecular Separators, Concentrators, and Detectors Preparatory to Sensor Operation, and Methods of Minimizing False Positives in Sensor Operations

As an elegant solution for minimizing false positives returned by a sensor tuned to an analyte molecule, filters constructed of carbon nanotubes are positioned relative to the sensor to limit the sensor to being exposed to molecules within a defined range of sizes, with too-big molecules being excluded from reaching the sensor by one filter, and too-small molecules being pumped out through another, finer filter before the sensor is operated. 13-. (canceled)4. The method of claim 11 , comprising:screening the initial sample to produce a screened sample consisting of molecules of size smaller than a maximum size; andrequiring molecules of size smaller than a minimum size to exit.5. The method of claim 4 , comprising producing a target sample consisting of molecules having a size in the predefined size range.6. The method of claim 4 , wherein the step of screening the initial sample to produce a screened sample consisting of molecules of size smaller than the maximum size includes applying a screen comprising at least one nanotube having an inner diameter to exclude molecules bigger than the maximum size.7. The method of claim 4 , wherein the step of requiring molecules of size greater than the minimum size to exit includes applying a screen comprising at least one nanotube having an inner diameter that permits passage of molecules below the minimum size.8. The method of claim 4 , comprising passing the initial sample through a screen comprising at least one nanotube having an inner diameter to exclude molecules bigger than the maximum size claim 4 ,followed by withdrawing, through a screen comprising at least one nanotube having an inner diameter that permits passage of molecules below the minimum size, a subset of molecules which are smaller than the minimum size.9. The method of claim 4 , comprising a screening step of screening an initial sample comprising at least one of an analyte molecule and/or a molecule that evokes a false positive for the analyte molecule.10 ...

APPARATUS AND METHOD FOR DISCONTINUOUS FILTRATION

The invention provides an apparatus for discontinuous filtration of a liquid, having a filter station for filtration, a feed line for supplying the liquid to the filter station, and a filter medium movable relative to the station, the filter medium comprising at least two different membranes, which together in the filter station effect multistage filtration. The invention additionally relates to a method for discontinuous filtration of a liquid or for filtering isolated quantities of liquid, comprising: providing a filter station for filtration, the filter station comprising a chamber in which filtration is carried out; moving or transporting a filter medium from a stock into the filter station, the filter medium comprising at least two membranes which are arranged next to one another in the chamber; and supplying the liquid to the chamber, such that the liquid is subjected to multistage filtration through the membranes. 1. Apparatus for discontinuous filtration of a liquid comprising: a filter station for carrying out filtration , a feed line for supplying a desired quantity of the liquid to be filtered to the filter station , and a filter medium movable relative to the filter station , wherein the filter medium comprises at least two different membranes , which together effect multistage filtration in the filter station.2. Apparatus according to claim 1 , wherein the filter medium comprises a first membrane and a second membrane claim 1 , each of said membranes forming a respective filtration stage claim 1 , the first and second membranes optionally being flat membranes and arranged next to one another in the filter station.3. Apparatus according to claim 2 , wherein the first membrane has a higher average and/or nominal pore size than the second membrane and is arranged upstream of the second membrane claim 2 , such that the first membrane may effect preliminary filtration of liquid.4. Apparatus according to claim 2 , wherein the first membrane and the second ...

NEW STORAGE, COLLECTION OR ISOLATION DEVICE

The present invention refers to a device, comprising a hollow body having at least one open end and at least one barrier inside or at the end of the hollow body, which is non-permeable for liquids and solids under ambience conditions, however, becomes liquid-permeable by applying an external force like pressure, drag force or driving power to said barrier, the use of such a device for collection, storage, treatment or isolation of a biomolecule or biomolecules containing samples, a method for preparation of said device and a method for isolation or purification of any biomolecules using said device. 117.-. (canceled)18. A device , comprising (i) at least one hollow body , each hollow body having at least one open end; (ii) at least one barrier placed inside or at one end of the hollow body , wherein the barrier is non-permeable for liquids and solids under ambience conditions , but becomes liquid-permeable by applying an external force to said barrier; (iii) optionally at least one liquid-permeable element inside the hollow body; and (iv) optionally at least one removable closing device to seal at least one open end.19. The device of claim 18 , wherein the external force is pressure claim 18 , drag force or driving power.20. The device of claim 18 , wherein the liquid-permeable element is a porous frit claim 18 , filter claim 18 , fleece claim 18 , or membrane.21. The device of claim 18 , comprising (i) at least one hollow body claim 18 , each hollow body having an inlet and an outlet; (ii) at least one barrier placed above the outlet or adjacent of at least one of the liquid permeable element(s) if present inside the hollow body; (iii) optionally at least one liquid-permeable element placed above the outlet; (iv) optionally at least one removable closing device to seal the inlet and/or outlet of the hollow body; and (v) optionally at least one collection tube to collect a mobile phase (eluate) after having passed the outlet.22. The device of claim 21 , the liquid- ...

PARTICLE NUMBER COUNTING APPARATUS

To improve an offset accuracy in a particle number counting apparatus () condensing a working liquid with sample particles, the apparatus including: a porous member () in which a flow path () is passed through to form an inlet (a) at a lower side and an outlet () at an upper side thereof so that the working liquid is supplied to a predetermined section of the porous member so as to be impregnated into the entire part of the porous member; and a housing () having an accommodating space for accommodating the porous member (), wherein a gap (S) is formed between an outer peripheral surface () of the porous member () and an inner peripheral surface () of the accommodating space of the housing () at least in an upper side than the predetermined section to be supplied with the working liquid. 1. A particle number counting apparatus comprising:a saturator part including a flow path in which a working liquid is diffused in a vaporized state so as to render sample particles contained in a measurement targeted gas to flow in the flow path;a condenser part for introducing the sample particles and vaporized working liquid from the flow path and condensing the working liquid with the sample particles as cores so as to produce droplets of the working liquid; anda counter part for counting the droplets of the working liquid,wherein the saturator part includes a porous member in which the flow path is passed through to form an inlet at a lower side and an outlet at an upper side thereof so that the working liquid is supplied to a predetermined section of the porous member so as to be impregnated into the entire part of the porous member, and a housing having an accommodating space for accommodating the porous member,wherein a gap is formed between an outer peripheral surface of the porous member and an inner peripheral surface of the accommodating space of the housing at least in an upper side than the predetermined section of the porous member to be supplied with the working ...

STOOL COLLECTION METHODS FOR ANALYSIS OF DNA IN STOOL

Methods of stool sample collection for improving DNA extraction and analysis from stool samples. More specifically, collecting stool samples from the same patient on two or more days (consecutively or randomly) and combining those stool samples for improved DNA extraction and analysis. 1. A stool collection method for analysis of human DNA in stool , the method comprising:(a) Collecting multiple stool samples from a person one or more times on consecutive days or random multiple days; and(b) Combining said multiple stool samples before human DNA analysis; and(c) Performing DNA analysis of said human DNA.2. The method of further comprises one or more DNA extraction and purification steps.3. The method of wherein said human DNA analysis further comprises one or more DNA analysis methods.4. The method of claim 3 , wherein said one or more human DNA analysis methods is selected from the group consisting of quantification of DNA claim 3 , measurement of the DNA fragment size claim 3 , and DNA mutation analysis.5. The method of claim 3 , wherein said one or more human DNA analysis methods is DNA methylation analysis.6. The method of further comprising a bisulfate treatment step before said DNA methylation analysis. The present application claims priority to and the benefit of U.S. provisional patent application number U.S. 61/589,847 filed Jan. 23, 2012, the entire disclosure of which is incorporated herein by reference.The invention provides stool collection methods for analysis of deoxyribonucleic acid (“DNA”) in stool.Colorectal cancer (CRC) is the second deadliest cancer in the US and the early detection is still the best way to eradicate it. It is recommended that all Americans who are 50 years of age or older should undergo CRC screening. Fecal occult blood tests (FOBTs) have been used to screen CRC for many years and continue to be one of the most frequently used screening tools. However, its screening accuracy is marginal. Due to limitations of FOBTs, colonoscopy ...

Compositions and methods for adhesion-based cell sorting

In an aspect, provided is an apparatus for sorting cells. The device may include polymer nanofibers treated with gaseous plasma. The nanofibers may comprise at least one of polycaprolactone and collagen. The gaseous plasma may comprise at least one of CF 4 , oxygen, argon, nitrogen, and air. In a further aspect, provided are methods of sorting cells in a composition. The method may include providing a substrate comprising polymer nanofibers that have been pretreated with a gaseous plasma, contacting the polymer nanofibers with a composition comprising a plurality of cells, and applying a force to the polymer nanofibers. In another aspect, provided are methods of making a device for sorting cells. The method may include applying a composition comprising at least one polymer onto a surface by electrospinning to form polymer nanofibers, and exposing the polymer nanofibers to a gaseous plasma to produce treated polymer nanofibers.

ORGANIC-INORGANIC HYBRID CHIRAL SORBENT AND PROCESS FOR THE PREPARATION THEREOF

The present invention provides an organic-inorganic hybrid chiral sorbent for chiral resolution of various racemic compounds viz. racemic mandelic acid, 2-phenyl propionic acid, diethyl tartrate, 2,2′-dihydroxy-1,1′-binaphthalene (BINOL) and cyano chromene oxide with excellent chiral separation (enantiomeric excess, 99%) in case of mandelic acid under medium pressure column chromatography. These optically pure enantiomers find applications as intermediates in pharmaceutical industries. 119.-. (canceled)20. A process for preparation of an organic-inorganic hybrid chiral sorbent comprising an anilino alcohol or a substituted anilino alcohol covalently bonded to a surface of a mesoporous silica material , the process comprising the steps of:(a) silylating a chiral epoxide with a silylating agent in an organic solvent with a molar ratio of the chiral epoxide to the silylating agent of about 1:1 to 1:2.5 in the presence of an inorganic base;(b) refluxing a reaction mixture obtained in the step (a) under an inert atmosphere for a period of 8 to 16 hours, followed by filtration to obtain a resultant filtrate;(c) refluxing the resultant filtrate obtained in the step (b) with the mesoporous silica, under inert atmosphere for a period of about 35 to 55 hours, followed by filtration and washing of a resultant solid product with toluene; and(d) reacting a resultant washed product obtained in the step (c) with aniline or substituted aniline in toluene, under reflux, under inert atmosphere for a period of 8 to 16 hours, followed by filtration and washing off a resultant reaction product with toluene and extracting the organic-inorganic hybrid chiral sorbent.21. The process according to claim 20 , wherein the chiral epoxide in the step (a) is selected from the group consisting of propene oxide claim 20 , 1-chloro-2 claim 20 ,3-epoxypropane claim 20 , 1-fluoro-2 claim 20 ,3-epoxypropane claim 20 , 1-bromo-2 claim 20 ,3-epoxypropane claim 20 , 1-methyl-2 claim 20 ,3-epoxypropane ...

High purity chromatographic materials comprising an ionizable modifier

The present invention provides novel chromatographic materials, e.g., for chromatographic separations, processes for its preparation and separations devices containing the chromatographic material; separations devices, chromatographic columns and kits comprising the same; and methods for the preparation thereof. The chromatographic materials of the invention are high purity chromatographic materials comprising a chromatographic surface wherein the chromatographic surface comprises a hydrophobic surface group and one or more ionizable modifier.

POROUS INORGANIC/ORGANIC HYBRID MONOLITH MATERIALS FOR CHROMATOGRAPHIC SEPARATIONS AND PROCESS FOR THEIR PREPARATION

Novel materials for chromatographic separations, processes for their preparation, and separation devices containing the chromatographic materials. In particular, the novel materials are porous inorganic/organic hybrid monolith materials, which desirably may be surface modified, and which offer more efficient chromatographic separations than that known in the art. 1134-. (canceled)135. A method of preparation of a porous inorganic/organic hybrid monolith material , comprising coalesced porous particles of hybrid silica having a chromatography-enhancing pore geometry , comprising the steps ofa) prepolymerizing a mixture of one or more organoalkoxysilanes and a tetraalkoxysilane in the presence of an acid catalyst to produce a polyorganoalkoxysiloxane;b) preparing an aqueous suspension of said polyorganoalkoxysiloxane, said suspension further comprising a surfactant or combination of surfactants, and gelling in the presence of a base catalyst so as to produce porous hybrid particles;c) modifying the pore structure of said porous hybrid particles by hydrothermal treatment; andd) coalescing said porous hybrid particles to form a monolith material thereby preparing a porous inorganic/organic hybrid monolith material.136152-. (canceled)153. A porous inorganic/organic hybrid monolith material comprising coalesced porous hybrid particles of hybrid silica having a chromatographically-enhancing pore geometry , produced by the process ofa) prepolymerizing a mixture of one or more organoalkoxysilanes and a tetraalkoxysilane in the presence of an acid catalyst to produce a polyorganoalkoxysiloxane;b) preparing an aqueous suspension of said polyorganoalkoxysiloxane, said suspension further comprising a surfactant or a combination of surfactants, and gelling in the presence of a base catalyst so as to produce porous hybrid particles;c) modifying the pore structure of said porous hybrid particles by hydrothermal treatment; andcoalescing said porous hybrid particles to form a ...

ZWITTERIONIC STATIONARY PHASE FOR HYDROPHILIC INTERACTION LIQUID CHROMATOGRAPHY AND PREPARATION METHOD THEREOF

A type of liquid chromatographic stationary phase and preparation method thereof, the bonding terminal of the chromatographic stationary phase is zwitterionic functional group. The preparation method includes the following steps, alkenyl or alkynyl silane is bonded onto the surface of silica based on the horizontal polymerization approach to obtain alkenyl- or alkynyl-modified silica. Then the thiol click reaction with zwitterionic compound containing thiol group is performed to obtain the zwitterionic hydrophilic interaction chromatographic stationary phase. The present stationary phase possesses both zwitterionic characteristics and excellent hydrophilicity. It can be widely applied in the separation of variety of samples. 2. A preparation method for the stationary phases as described in claim 1 , characterized in that:a. Pretreatment of silica: after adding hydrochloric acid or nitric acid aqueous solution with the concentration of 1˜38 wt % into silica gel, the solution is heated to reflux while stirring for 1˜48 hours. The resulted material is filtered, washed with water until neutral and dried to constant weight under 100˜160° C. After that, the dried silica gel is placed in nitrogen or argon atmosphere with the humidity of 20˜80% for 24˜72 hours until the weight increment is 0.5˜10 wt % to obtain the humidified silica.b. Polymerization on silica surface: the humidified silica obtained from step a is first placed in reaction vessel made of glass or polytetrafluoroethylene, and organic solvent is added under nitrogen atmosphere. The solution is stirred and mixed well, and then alkenyl or alkynyl silane is dropwise added. The reaction is performed under 20˜200° C. for 2˜48 hours with continuous stirring. After that, the reaction is cooled to room temperature. The reaction product is filtered and washed by toluene, dichloromethane, methanol, water, tetrahydrofuran and methanol successively. The solid product is dried under 60˜100° C. for 12˜24 hours to obtain ...

PORTABLE DEVICE FOR THE STORAGE, TRANSPORT AND RECUPERATION OF BIOLOGICAL MATERIAL

The present invention refers to a portable device for the collection of biological samples. The device comprises a receptacle () with a cover (), which comprises a pierceable area () and may include a collection instrument (), comprising a rod (), and a collection arrangement at the end, which may include a solid matrix, for instance a sponge or a tampon (). The receptacle () has a rounded or conical shaped bottom (), at the center of which there is a conical or rounded-shaped projection (), with one or more holes (). The bottom of the receptacle () is in contact with a collector recipient through a system of close-aperture, which may comprise screw or a fitting system with protrusions (). In this way, the device referred in the present invention allows for the collection, storage, transportation and separation of biological material without significant manipulation and without risk of cross-contamination. 1. Device for collection and treatment of biological samples comprising:{'b': 8', '10', '11', '12, 'a receptacle () with a conical or round-shaped end (), at the center of which there is a conical cr roundedshape protuberance () and one or more holes ();'}{'b': 2', '3, 'a cover (), which comprises a pierceable area ();'}{'b': 14', '8, 'a collector recipient () that contacts with one end of the receptacle ().'}22145. Device claim 1 , according to claim 1 , further comprising a cover () comprising a collection instrument () claim 1 , which includes a rod () and a collection arrangement () at one end.35. Device claim 2 , according to claim 2 , further comprising a collection arrangement () comprising a solid matrix composed of an absorbing material.481413. Device claim 1 , according to claim 1 , further comprising a receptacle () which contacts with a collector recipient () through a close-aperture system that comprises a fitting system with protrusions ().5. Device claim 1 , according to claim 1 , characterized by being used in collection and/or storage of ...

Specific sorbent for binding proteins and peptides, and separation method using the same

Sorbent comprising a solid support material, the surface of which comprises first residues comprising a binuclear heteroaromatic structure comprising besides carbon atoms at least one of the heteroatoms N, O, S, and second residues comprising a mononuclear heteroaromatic structure comprising besides carbon atoms at least one of the heteroatoms N, O, S.

Method of making ionic liquid mediated sol-gel sorbents

Ionic liquid (IL)-mediated sol-gel hybrid organic-inorganic materials present enormous potential for effective use in analytical microextraction. One obstacle to materializing this prospect arises from high viscosity of ILs significantly slowing down sol-gel reactions. A method was developed which provides phosphonium-based, pyridinium-based, and imidazolium-based IL-mediated advanced sol-gel organic-inorganic hybrid materials for capillary microextraction. Scanning electron microscopy results demonstrate that ILs can serve as porogenic agents in sol-gel reactions. IL-mediated sol-gel coatings prepared with silanol-terminated polymers provided up to 28 times higher extractions compared to analogous sol-gel coatings prepared without any IL in the sol solution. This study shows that IL-generated porous morphology alone is not enough to provide effective extraction media: careful choice of the organic polymer and the precursor with close sol-gel reactivity must be made to ensure effective chemical bonding of the organic polymer to the created sol-gel material to be able to provide the desired sorbent characteristics.

Monolithic silicone and method of separation, purification and concentration therewith

The present invention provides a monolithic silicone in the form of an aerogel or a xerogel having flexibility and capable of dissolving molecules of a substance. This silicone monolithic body having continuous through passages is synthesized by copolymerizing starting materials of both a bifunctional alkoxysilane and a trifunctional alkoxysilane or tri- or higher functional alkoxysilanes through a sol-gel reaction for forming a Si—O network while causing phase separation.

MAGNETOPHORETIC ANALYTE SELECTION AND CONCENTRATION

Devices and methods for magnetophoretic analyte selection and concentration are described. Magnetically marked analytes (e.g., cells) may be separated out of a sample dynamically in flux, such that the magnetically marked analytes are present in a highly concentrated manner in a reduced sample volume. The analyte selection may be followed by an analysis. 1. A device for magnetophoretic analyte selection and enrichment , the device comprising:a flow channel;a first magnetic unit configured for the enrichment; anda second magnetic unit configured for aligning magnetically marked analytes,wherein the first magnetic unit and the second magnetic unit are arranged in a first portion of the flow channel,wherein the flow channel splits into at least a first part-channel and a second part-channel in a second portion of the flow channel, andwherein in a third portion of the flow channel, the first part-channel has a cross-sectional area that is smaller than a cross-sectional area of the flow channel in the first portion.2. The device as claimed in claim 1 , wherein the first part-channel has a cross-sectional area in the third portion of the flow channel that is less than one-half of the cross-sectional area of the flow channel in the first portion of the flow channel.3. The device as claimed in claim 1 , wherein the second part-channel has a cross-sectional area claim 1 , or multiple second part-channels have a total cross-sectional area that is sufficiently large to transport a sample volume arriving through the flow channel in the first portion to the first part-channel and the second part-channel in the third portion.4. The device as claimed in claim 3 , wherein the cross-sectional area of the second part-channel or the total cross-sectional area of multiple second part-channels sufficiently large that a flow behavior of the sample volume is substantially uninfluenced claim 3 , and the enrichment and the aligning of the magnetically marked analytes in the sample volume is ...

METAL OXIDE-BASED BIOCOMPATIBLE HYBRID SORBENT FOR THE EXTRACTION AND ENRICHMENT OF CATECHOLAMINE NEUROTRANSMITTERS AND RELATED COMPOUNDS, AND METHOD OF SYNTHESIS

The subject invention concerns metal or metalloid oxide-based sol-gel hybrid sorbent and methods of synthesis. In one embodiment, the sorbent is a ZrOpolypropylene oxide based sol-gel. The subject invention also concerns a hollow tube or capillary internally coated with a sorbent of the invention. Sorbent coated tubes and capillaries of the invention can be used in extraction and/or enrichment of samples to be analyzed for catecholamines and related compounds. 1. A metal or metalloid oxide-based sol-gel hybrid sorbent composition prepared from a biocompatible polymer or ligand comprising one or more sol-gel active end groups.2. The sorbent composition according to claim 1 , wherein the metal or metalloid of the sorbent composition is aluminum claim 1 , antimony claim 1 , arsenic claim 1 , barium claim 1 , beryllium claim 1 , bismuth claim 1 , boron claim 1 , cadmium claim 1 , cerium claim 1 , chromium claim 1 , cobalt claim 1 , copper claim 1 , dysprosium claim 1 , erbium claim 1 , europium claim 1 , gadolinium claim 1 , gallium claim 1 , gold claim 1 , hafnium claim 1 , holmium claim 1 , indium claim 1 , iridium claim 1 , iron claim 1 , lanthanum claim 1 , lithium claim 1 , magnesium claim 1 , manganese claim 1 , molybdenum claim 1 , neodymium claim 1 , nickel claim 1 , niobium claim 1 , osmium claim 1 , palladium claim 1 , platinum claim 1 , praseodymium claim 1 , rhodium claim 1 , ruthenium claim 1 , samarium claim 1 , scandium claim 1 , selenium claim 1 , silver claim 1 , strontium claim 1 , tellurium claim 1 , terbium claim 1 , thallium claim 1 , thulium titanium claim 1 , tantalum claim 1 , vanadium claim 1 , yttrium claim 1 , zirconium claim 1 , zinc claim 1 , tungsten claim 1 , or any combination thereof.3. The sorbent composition according to claim 1 , wherein the sorbent composition comprises ZrOpolypropylene oxide (ZrOPPO).5. The sorbent composition according to claim 1 , wherein the polymer or ligand used to prepare the sorbent composition comprises one ...

Stationary phase for supercritical fluid chromatography

Provided is a stationary phase for supercritical fluid chromatography that includes a carrier on which is supported a polymer that includes a pyrrolidone backbone in the repeating units of the main chain.

MULTI-SITE PARTICLE SENSING SYSTEM

A particle sensing system which includes a plurality of micro-lenses which focus light from an unfocused or loosely focused light source onto a corresponding plurality of focus regions on a surface containing plasmonic structures. The absorption of light by the plasmonic structures in the focus regions results in heat dissipation in the plasmonic structures and consequently increases surface temperature in the focus regions. When an electrical field is applied to a sample fluid in contact with the surface, multiple electrothermal flows are induced in the fluid which rapidly transport suspended particles to the focus regions on the surface. The particles can then be captured and/or sensed. 1. A particle sensing system , comprising:a first substrate having a first conductive layer on a first side of the first substrate, and a plurality of microlenses mounted to the first conductive layer;a second substrate having a second conductive layer, the second conducting layer facing the first conductive layer, the second conductive layer having a plurality of light absorbing plasmonic structures; andat least one channel separating the first and second conductive layers and configured to hold a liquid sample;wherein the microlenses are configured to create a plurality of focus regions on the second conductive layer when light is directed through the microlenses.2. The system of claim 1 , further comprising an alternating current (AC) source connected between the first and second conductive layers to induce an electric field in the liquid sample.3. The system of claim 1 , wherein the distance between the microlenses and the second conductive layer is chosen to be equal to a focal length of the microlenses.4. The system of claim 1 , wherein the plurality of microlenses are arranged in arrays on the first conductive layer.5. The system of claim 1 , wherein a thin dielectric spacer layer is placed between the microlenses and the first conductive layer.6. The system of claim 1 , ...

INTEGRATED HIGH THROUGHPUT SYSTEM FOR THE ANALYSIS OF BIOMOLECULES

Described is an affinity microcolumn comprising a high surface area material, which has high flow properties and a low dead volume, contained within a housing and having affinity reagents bound to the surface of the high surface area material that are either activated or activatable. The affinity reagents bound to the surface of the affinity microcolumn further comprise affinity receptors for the integration into high throughput analysis of biomolecules. 1. An affinity microcolumn comprising a polymer contained within a housing and affinity reagents bound to the polymer , wherein the affinity reagents are either activated or activatable.2. The affinity microcolumn of wherein the polymer is formed by molding.3. The affinity microcolumn of wherein the polymer is exposed to at least one of chemical etching and electro etching.4. The affinity microcolumn of wherein the affinity reagents that are bound to the polymer further comprise affinity receptors bound to the affinity reagents.5. The affinity microcolumn of further comprising a tethering molecule that is activated or activatable and binds the affinity receptors to the affinity reagents.6. The affinity microcolumn of further comprising an amplification media that is activated or activatable and is interposed between the affinity reagents and the affinity receptors claim 4 , where the amplification media allows a high density of affinity receptors to be bound to the affinity reagents than in the absence of the amplification media.7. The affinity microcolumn of further comprising an amplification media interposed between the affinity reagents and the affinity receptors claim 4 , where the amplification media allows better access by an analyte to the affinity receptors than in the absence of the amplification media.8. The affinity microcolumn of wherein the amplification media comprises at least one of a biological polymer claim 6 , a non-biological organic polymer claim 6 , and an inorganic polymer.9. The affinity ...

Methods for predicting overall and progression free survival in subjects having cancer using circulating cancer associated macrophage-like cells (camls)

Means for predicting overall survival (OS) and progression free survival (PFS) of subjects having cancer are disclosed, where the predictions are based on the number arid size of circulating cancer associated macrophage-like cells (CAMLs) found in a biological sample, such as blood, from the subject.

PACKING MATERIAL FOR LIQUID CHROMATOGRAPHY AND COLUMN FOR LIQUID CHROMATOGRAPHY

A packing material for liquid chromatography, which is excellent in durability, and a column for liquid chromatography, which is filled with the packing material, are provided. The packing material for liquid chromatography is characterized by comprising a hydrophilic resin containing a polyvinyl alcohol resin, to said hydrophilic resin an amino group represented by the formula (1) having been bonded through a spacer. In the formula (1), Rrepresents a hydrogen atom or an alkyl group having 1 to 4 carbon atoms, Rrepresents an alkyl group having 1 to 6 carbon atoms and having one or more hydroxyl groups, and  represents a bonding position to the spacer. 2. The packing material for liquid chromatography as claimed in claim 1 , wherein Ris a hydrogen atom or a methyl group.4. The packing material for liquid chromatography as claimed in claim 1 , wherein the amino group represented by the formula (1) is derived from any one amine of the group consisting of D-glucamine claim 1 , N-methyl-D-glucamine claim 1 , 1-amino-1-deoxy-D-mannitol claim 1 , 1-amino-1-deoxy-D-galactitol claim 1 , 1-amino-1-deoxy-D-iditol claim 1 , 1-amino-1-deoxy-D-arabinitol claim 1 , 1-amino-1-deoxy-D-xylitol claim 1 , 4-amino-1 claim 1 ,2 claim 1 ,3-butanetriol claim 1 , 3-amino-1 claim 1 ,2-propanediol and 3-methylamino-1 claim 1 ,2-propanediol.5. The packing material for liquid chromatography as claimed in claim 1 , wherein the spacer is derived from a compound having a glycidyl group at an end.6. A column for liquid chromatography claim 1 , using the packing material for liquid chromatography as claimed in .7. A column for liquid chromatography for saccharide analysis claim 1 , using the packing material for liquid chromatography as claimed in .8. A column for hydrophilic interaction chromatography claim 1 , using the packing material for liquid chromatography as claimed in . The present invention relates to a packing material for liquid chromatography, which is excellent in durability, and a ...

A MICROFLUIDIC DEVICE AND METHODS FOR MANUFACTURING SAME

The present invention relates generally to a sample processing device, such as a microfluidic device, comprising a substrate, wherein the substrate comprises a plurality of channels configured to transport a fluid, and wherein the plurality of channels are substantially coated with lubricin, or a functional variant thereof. Also disclosed herein are methods of manufacturing such devices, methods of preventing fouling of a channel in a device using lubricin, or a functional variant thereof and methods of controlling the electrokinetic flow of an analyte through a channel that is substantially coated with lubricin, or a functional variant thereof. Also disclosed herein is chromatographic material for the electrophoretic and/or chromatographic separation of an analyte, wherein the chromatographic material is substantially coated with lubricin, or a functional variant thereof. 1. A microfluidic device comprising a substrate , wherein the substrate comprises a plurality of channels configured to transport a fluid , and wherein the plurality of channels are substantially coated with lubricin , or a functional variant thereof.2. The device of claim 1 , wherein the plurality of channels comprise a surface selected from the group consisting of a hydrophobic surface claim 1 , an anionic surface claim 1 , a polar surface and combinations thereof.3. The device of claim 2 , wherein the plurality of channels comprise a hydrophobic surface.4. The device of claim 3 , wherein the hydrophobic surface is a gold surface.5. The device of claim 3 , wherein the hydrophobic surface is a thiol-modified hydrophobic surface.6. The device of claim 5 , wherein the thiol is a OH-thiol or a CH-thiol.7. The device of claim 1 , wherein the plurality of channels comprise a COOH-thiol-modified surface.8. The device of claim 1 , wherein the plurality of channels comprise a polystyrene-modified surface.9. The device of claim 1 , wherein at least one of the plurality of channels is a capillary.10. The ...

POROUS CHIRAL MATERIALS AND USES THEREOF

A porous chiral material of formula [M(L)(A)]Xwherein M is a metal ion; L is a nitrogen-containing bidentate ligand; A is the anion of mandelic acid or a related acid; and Xis an anion 1. A porous chiral material of formula [M(L)(A)]X wherein M is a metal ion; L is a nitrogen-containing bidentate ligand; A is the anion of mandelic acid or a related acid; and X is an anion.2. A porous chiral material according to wherein M is selected from a group consisting of: cobalt claim 1 , chromium claim 1 , iron claim 1 , nickel claim 1 , manganese claim 1 , calcium claim 1 , magnesium claim 1 , cadmium claim 1 , copper and zinc.3. A porous chiral material according to wherein L is selected from a group consisting of: 4 claim 1 ,4′-bipyridine claim 1 , 1 claim 1 ,2-bis(4-pyridyl)ethane claim 1 , and 4 claim 1 ,4′-bipyridylacetylene.4. A porous chiral material according to wherein A is the anion of (S)-(−)-mandelic acid.5. A porous chiral material according to wherein X is a triflate ion.6. (canceled)7. A material of formula [M(L)(A)]XGwherein M is a metal ion; L is a nitrogen-containing bidentate ligand; A is an anion of mandelic acid or a related acid; X is an organic anion; G is a guest molecule; and n is from 0 to 5.8. (canceled)9. A crystalline sponge comprising a porous chiral material of formula [M(L)(A)]X.10. A method of separating enantiomers claim 1 , the method comprising contacting a composition comprising a mixture of enantiomers with a material of .11. (canceled)12. A method of separating enantiomers according to claim 10 , the method comprising passing a composition comprising the mixture of enantiomers through a chromatography column comprising as a stationary phase a chiral porous material of formula [M(L)(A)]X wherein M is a metal ion; L is a nitrogen-containing bidentate ligand; A is an anion of mandelic acid or a related acid; and X is an anion.13. A method of separating enantiomers according to claim 12 , the method comprising:{'sub': '1.5', 'sup': +', '−, ...

BIOLOGICAL DETECTION SYSTEM AND BIOLOGICAL DETECTION DEVICE

A biological detection system includes a control module, a bearing rotatable plate, a first driving module, rotatable sub-plates, second driving modules, and test cassettes. The bearing rotatable plate has a main rotating shaft. The first driving module is electrically connected to the control module and connected to the main rotating shaft, so that the bearing rotatable plate rotates about the main rotating shaft. The rotatable sub-plates each has a respective independent rotating shaft. The rotatable sub-plates are rotatably disposed on the bearing rotatable plate about the respective independent rotating shaft. The independent rotating shafts and the main rotating shaft may have different rotating directions and rotating speeds. The second driving modules are electrically connected to the control module, so that the rotatable sub-plates independently rotate about the respective independent rotating shaft. The test cassettes are detachably disposed on the rotatable sub-plates, and each of the test cassettes includes a micro-channel structure. 1. A biological detection system , comprising:a control module;a bearing rotatable plate, having a main rotating shaft;a first driving module, electrically connected to the control module and connected to the main rotating shaft, and adapted for the bearing rotatable plate to rotate about the main rotating shaft;a plurality of rotatable sub-plates, each having a respective independent rotating shaft different from the main rotating shaft, wherein the rotatable sub-plates are disposed on the bearing rotatable plate and each is independently rotatable about the respective independent rotating shaft;a plurality of second driving modules, electrically connected to the control module, so that each of the rotatable sub-plates independently rotates about the respective independent rotating shaft; anda plurality of test cassettes, detachably disposed on the rotatable sub-plates, wherein the each of the test cassettes comprises a ...

POLYETHYLENE TEREPHTHALATE (PET) AEROGEL

A polyethylene terephthalate aerogel. There is provided a polyethylene terephthalate (PET) aerogel comprising a porous network of cross-linked recycled PET fibers, wherein the PET aerogel has a thermal conductivity of 0.030-0.050 W/m K. There is also provided a method of forming the PET aerogel. 1. A polyethylene terephthalate (PET) aerogel cors 1prising a porous network of cross-linked recycled PET fibers , wherein the PET aerogel has a thermal conductivity of 0.030-0.050 W/m K.21. The PET aerogel according to claim 1 , wherein the recycled PET fibers comprised in the PET aerogel are obtained from PET plastic bottles.3. The PET aerogel according to claim 1 , wherein the cross-linked recycled PET fibers comprised in the aerogel are cross-linked with a cross-linker selected from: tetraethoxysilane (TEOS) claim 1 , polyvinyl alcohol (PVA) claim 1 , glutaraldehyde (GA) claim 1 , methyltrimethoxysilane (MTMS) claim 1 , sodium silicate claim 1 , bentonite claim 1 , starch claim 1 , nanoclay claim 1 , or a combination thereof.4. The PET aerogel according to claim 3 , wherein the cross-linker is TEOS.5. The PET aerogel according to claim 3 , wherein the cross-linker is a combination of PVA and GA.6. The PET aerogel according to claim 1 , wherein the PET aerogel has a density of 0.007-0.450 g/cm.7. The PET aerogel according to claim 1 , wherein the PET aerogel has a compressive Young's modulus of ≤130.0 kPa.8. The PET aerogel according to claim 1 , wherein the PET aerogel is superhydrophobic and has a contact angle of 120-150°.9. A method of forming the PET aerogel according to claim 1 , the method comprising:hydrolysing recycled PET fibers to form hydrolysed recycled PET fibers, wherein the hydrolysing forms at least carboxylic groups on a surface of the hydrolysed recycled PET fibers;cross-linking the hydrolysed recycled PET fibers with a cross-linker;gelation of the cross-linked recycled PET fibers; anddrying to form the PET aerogel.10. The method according to claim 9 , ...

MATERIALS FOR HYDROPHILIC INTERACTION CHROMATOGRAPHY AND PROCESSES FOR PREPARATION AND USE THEREOF FOR ANALYSIS OF GLYCOPROTEINS AND GLYCOPEPTIDES

The invention relates to poly-amide bonded hydrophilic interaction chromatography (HILIC) stationary phases and novel HILIC methods for use in the characterization of large biological molecules modified with polar groups, known to those skilled in the art as glycans. The invention particularly provides novel, poly-amide bonded materials designed for efficient separation of large biomolecules, e.g. materials having a large percentage of larger pores (i.e. wide pores). Furthermore, the invention advantageously provides novel HILIC methods that can be used in combination with the stationary phase materials described herein to effectively separate protein and peptide glycoforms by eliminating previously unsolved problems, such as on-column aggregation of protein samples, low sensitivity of chromatographic detection of the glycan moieties, and low resolution of peaks due to restricted pore diffusion and long intra/inter-particle diffusion distances. 149-. (canceled)50. A method for analyzing a glycosylated proteinaceous sample , comprising contacting the sample with a chromatographic material in the presence of a mobile phase eluent to thereby analyze the sample , wherein the chromatographic material is a porous material which comprises at least one hydrophilic monomer and a poly-amide bonded phase.51. The method of claim 50 , wherein an average pore diameter of the porous material is greater than or equal to about 200 Å.52. The method of claim 50 , wherein an average pore diameter of the porous material is ranges from about 200 Å to about 450 Å.53. The method of claim 50 , wherein the porous material has an average pore diameter of about 300 Å.54. The method of claim 50 , wherein the mobile phase eluent is a high organic eluent.55. The method of claim 50 , wherein the mobile phase eluent comprises acetonitrile claim 50 , isopropanol claim 50 , n-propanol claim 50 , methanol claim 50 , ethanol claim 50 , butanol claim 50 , water claim 50 , or a mixture thereof.56. The ...

ELECTRO-SPUN FIBERS AND APPLICATIONS THEREOF

A supported nanofiber medium useful for segregating chemical species is provided by selecting a polymer, selecting a substrate; and electrospinning the polymer to form a nanofiber medium on the supporting substrate. When the substrate is a planar surface, the nanofiber medium will be a mat suitable for conducting chromatographic separation. When the substrate is a filament, the nanofiber medium is an annular mat suitable for solid phase microextraction. The nanofiber media formed may be selectively cross-linked and at least partially carbonized to carbon nanofibers. The nanofiber medium is supported on the substrate without the use of binder material. 1. A method for analytically separating at least two chemical species , comprising the steps of:providing a separation medium, comprising a mat of nanofibers disposed on a surface of a substrate;providing at least two chemical species, mixed together in an appropriate solvent; andseparating the at least two chemical species from each other through contact of the mixture with the separation medium.2. The method of claim 1 , wherein:the separating step is achieved by ultrathin layer chromatography (UTLC);the substrate surface is planar and the mat of nanofibers on the planar surface has a thickness in the range of from about 11.5 to about 17.4 microns and has an average fiber diameter in the range of from about 150 to about 400 nm, such that the separation medium is a UTLC stationary phase; andthe mixture of the at least two chemical species in the appropriate solvent is a UTLC mobile phase.3. The method of claim 2 , wherein: placing the UTLC mobile phase onto the UTLC stationary phase; and', 'drawing the UTLC mobile phase upward by capillary action., 'the ultra-thin chromatography separating step is achieved by the steps of4. The method of claim 1 , wherein: placing the UTLC mobile phase onto the UTLC stationary phase; and', 'drawing the UTLC mobile phase upward by capillary action., 'the ultra-thin chromatography ...

PACKING MATERIAL FOR LIQUID CHROMATOGRAPHY AND COLUMN FOR LIQUID CHROMATOGRAPHY

A packing material for liquid chromatography, which is excellent in durability, and a column for liquid chromatography, which is filled with the packing material, are provided. The packing material for liquid chromatography is characterized by comprising a hydrophilic resin containing a polyvinyl alcohol resin, to said hydrophilic resin an amino group represented by the formula (1) having been bonded through a spacer. In the formula (1), Rrepresents a hydrogen atom or an alkyl group having 1 to 4 carbon atoms, Rrepresents an alkyl group having 1 to 6 carbon atoms and having one or more hydroxyl groups, and ※ represents a bonding position to the spacer. 2. The packing material for liquid chromatography as claimed in claim 1 , wherein Ris a hydrogen atom or a methyl group.4. The packing material for liquid chromatography as claimed in claim 1 , wherein the amino group represented by the formula (1) is derived from any one amine of the group consisting of D-glucamine claim 1 , N-methyl-D-glucamine claim 1 , 1-amino-1-deoxy-D-mannitol claim 1 , 1-amino-1-deoxy-D-galactitol claim 1 , 1-amino-1-deoxy-D-iditol claim 1 , 1-amino-1-deoxy-D-arabinitol claim 1 , 1-amino-1-deoxy-D-xylitol claim 1 , 4-amino-1 claim 1 ,2 claim 1 ,3-butanetriol claim 1 , 3-amino-1 claim 1 ,2-propanediol and 3-methylamino-1 claim 1 ,2-propanediol.5. The packing material for liquid chromatography as claimed in claim 1 , wherein the spacer is derived from a compound having a glycidyl group at an end.6. A column for liquid chromatography claim 1 , using the packing material for liquid chromatography as claimed in . This is a divisional of U.S. application Ser. No. 15/121,806 filed Aug. 26, 2016, which is a National Stage of International Application No. PCT/JP2015/055031, filed Feb. 23, 2015, claiming priority based on Japanese Patent Application No. 2014-038331, filed Feb. 28, 2014, the contents of all of which are incorporated herein by reference in their entirety.The present invention relates to a ...

SUPERFICIALLY POROUS MATERIALS COMPRISING A COATED CORE HAVING NARROW PARTICLE SIZE DISTRIBUTION; PROCESS FOR THE PREPARATION THEREOF; AND USE THEREOF FOR CHROMATOGRAPHIC SEPARATIONS

The present invention provides novel chromatographic materials, e.g., for chromatographic separations, processes for its preparation and separations devices containing the chromatographic material; separations devices, chromatographic columns and kits comprising the same; and methods for the preparation thereof. The chromatographic materials of the invention are chromatographic materials comprising having a narrow particle size distribution. 1. A superficially porous material comprising a coated core and one or more layers of a porous shell material surrounding the coated core , wherein said coated core comprises a substantially nonporous core material coated with a core-coating material.2. The superficially porous material of claim 1 , wherein the material is comprised of superficially porous particles.3. The superficially porous material of claim 1 , wherein the material is a superficially porous monolith.4. The superficially porous material of claim 2 , wherein the material has a substantially narrow particle size distribution.5. The superficially porous material of claim 2 , wherein the substantially nonporous core material has a substantially narrow particle size distribution.6. The superficially porous material of claim 2 , wherein the 90/10 ratio of particle sizes is from 1.00-1.55.7. The superficially porous material of claim 6 , wherein the 90/10 ratio of particle sizes is from 1.00-1.10.8. The superficially porous material of claim 7 , wherein the 90/10 ratio of particle sizes is from 1.05-1.10.9. The superficially porous material of claim 6 , wherein the 90/10 ratio of particle sizes is from 1.10-1.55.10. The superficially porous material of claim 9 , wherein the 90/10 ratio of particle sizes is from 1.10-1.50.11. The superficially porous material of claim 10 , wherein the 90/10 ratio of particle sizes is from 1.30-1.45.12. The superficially porous material of claim 1 , wherein the material has chromatographically enhancing pore geometry.13. The ...

Method

There is provided a method of identifying a resin for isolating or enriching a protein of interest using affinity chromatography. The method comprises the steps of: i) providing the three-dimensional structure of the protein of interest; ii) determining and/or calculating one or more parameters of the protein of interest in its two- and/or three-dimensional form; iii) determining and/or calculating one or more parameters of one or more resin in their two- and/or three-dimensional form; and iv) selecting a resin expected to bind complementarily to the protein of interest based upon one or more of the parameters of the protein of interest.

ENDOTOXIN ADSORBENT

A means for selectively removing ET under coexistence of a substance showing a negative charge, such as nucleic acid is described. Endotoxin is selectively removed by bringing a polymer obtained by crosslinking cyclodextrin with an isocyanate-based crosslinking agent in contact with a solution containing endotoxin and the substance showing the negative charge such as nucleic acid. 1. A polymer of cyclodextrin , having features (1) to (4) described below: {'br': None, 'i': 'N/C', '(mol %)=nitrogen content (mol)/carbon content (mol)×100\u2003\u2003(formula 1)'}, '(1) N/C shown according the following formula is 6 to 15;'}(2) part of hydroxy group of cyclodextrin forms a urethane bond;(3) insoluble in water; and(4) anion exchange capacity is less than 0.1 meq/g.2. A method for producing the polymer according to claim 1 , comprising allowing a crosslinking agent to react with cyclodextrin claim 1 , whereinthe crosslinking agent is a compound having one or more isocyanate groups, per molecule, and one or more functional groups that can react with a hydroxy group.3. The method according to claim 2 , wherein the functional group that can react with the hydroxy group is an isocyanate group.4. An endotoxin adsorbent claim 1 , containing a base material and the polymer according to as immobilized to the base material.5. A method for producing an endotoxin adsorbent claim 1 , comprising immobilizing the polymer according to to a base material.6. The method for producing the endotoxin adsorbent according to claim 4 , comprising allowing a crosslinking agent to react with a base material claim 4 , and cyclodextrin claim 4 , wherein the crosslinking agent is a compound having one or more isocyanate groups claim 4 , and one or more functional groups that can react with a hydroxy group.7. The method according to claim 6 , wherein the functional group that can react with the hydroxy group is an isocyanate group. This application is a divisional application of and claims the priority ...

AMINOGLYCOSIDE HYDROGEL MICROBEADS AND MACROPOROUS GELS WITH CHEMICAL CROSSLINK, METHOD OF PREPARATION AND USE THEREOF

Methods and materials for the generation of amikacin antibiotic-derived microbeads, (FIG. ). These beads may function as anion-exchange resins for use in pDNA purification as well as in situ capture of DNA from mammalian cells. New microresin and macroporous monolith based materials also are disclosed and may function for plasmid DNA binding and purification, mammalian whole cell genomic DNA extraction, and in-vitro cell culture. 1. A method to prepare a macroporous column , comprising:providing a chromatographic column packed with salt;mixing an aminoglycoside and a cross-linker in an organic solvent;disposing said aminoglycoside/cross-linker/organic solvent in said chromatographic column;after said aminoglycoside reacts with said cross-linker to form a macroporous porous, cross-linked resin, adding water to said column to dissolve said salt;draining said water from said chromatographic column,2. The method of claim 1 , wherein said aminoglycoside is selected from the group consisting of Amikacin claim 1 , Neomycin claim 1 , Streptomycin claim 1 , Tobramycin claim 1 , Sisomicin claim 1 , Paromomycin claim 1 , Apramycin claim 1 , Framecytin claim 1 , Ribostamycin claim 1 , Kanamycin claim 1 , Arbekacin claim 1 , Beckanamycin claim 1 , Dibekacin claim 1 , Astromicin claim 1 , Spectinomycin claim 1 , Hygromycin b claim 1 , Gentamicin claim 1 , Netilmicin claim 1 , Isepamicin claim 1 , and Verdamicin.3. The method of claim 1 , wherein said cross-linker comprises a di-epoxide.4. The method of claim 1 , wherein said cross-linker is selected from the group consisting of Poly (ethylene glycol) diglycidyl ether claim 1 , Ethylene glycol diglycidyl ether claim 1 , 1 claim 1 , 4-Cyclohexane dimethanol diglycidyl ether claim 1 , Neopentyl glycol diglycidyl ether claim 1 , 1 claim 1 ,4-Butanediol diglycidyl ether claim 1 , Resorcinol diglycidyl ether claim 1 , Poly (propylene glycol) diglycidyl ether claim 1 , Glycerol diglycidyl ether claim 1 , Polyethylene glycol) diacrylate ...

HIGH RESOLUTION SYSTEMS, KITS, APPARATUS, AND METHODS USING MAGNETIC BEADS FOR HIGH THROUGHPUT MICROBIOLOGY APPLICATIONS

A method of transferring material from a first microfabricated device to a second microfabricated device. At least one magnetic bead is loaded into at least one microwell of the first microfabricated device, where a plurality of cells are cultivated. The second microfabricated device is positioned such that the at least one microwell of the first array of microwells is aligned with at least one microwell of the second array of microwells. A magnetic field is applied so as to move the at least one magnetic bead contained in the at least one microwell of the first microfabricated device into the at least one microwell of the second microfabricated device. In this manner, at least one cell from the plurality of cells in the at least one microwell of the first microfabricated device is transferred to the at least one microwell of the second microfabricated device. 1. A method of transferring material from a first microfabricated device including a first array of microwells to a second microfabricated device including a second array of microwells , the method comprising:loading at least one magnetic bead into at least one microwell of the first array of microwells;incubating the first microfabricated device to cultivate a plurality of cells in at least one microwell of the first array of microwells;positioning the second microfabricated device relative to the first microfabricated device such that the at least one microwell of the first array of microwells of the first microfabricated device is aligned with at least one microwell of the second array of microwells of the second microfabricated device; andapplying a magnetic field so as to move the at least one magnetic bead contained in the at least one microwell of the first array of microwells into the at least one microwell of the second array of microwells, whereby at least one cell from the plurality of cells in the at least one microwell of the first array of microwells is transferred to the at least one microwell ...

PARTICLE CONCENTRATION SYSTEM

Methods and systems are provided for concentrating particles (e.g., bacteria, viruses, cells, and nucleic acids) suspended in a liquid. Electric-field-induced forces urge the particles towards a first electrode immersed in the liquid. When the particles are in close proximity to (e.g., in contact with) the first electrode, the electrode is withdrawn from the liquid and capillary forces formed between the withdrawing electrode and the surface of the liquid immobilize the particles on the electrode. Upon withdrawal of the electrode from the liquid, the portion of the electrode previously immersed in the liquid has particles immobilized on its surface. 1. A particle concentrating system , comprising:(a) a first electrode having a high aspect ratio, wherein the first electrode comprises a shaft having a shaft latitudinal dimension and a distal end having a distal latitudinal dimension, wherein the distal latitudinal dimension is from one nanometer to one millimeter;(b) an actuator configured to immerse and withdraw the first electrode from the first liquid such that a capillary force formed between the withdrawing first electrode and the first liquid immobilizes the first particle on a surface of the first electrode; and(c) an electric signal generator configured to generate an electrically induced force through the first electrode such that when the first electrode is immersed in a first liquid, a first particle in the first liquid is preferentially urged toward the first electrode.2. The system of claim 1 , further comprising the first liquid comprising the first particle.3. The system of claim 1 , wherein the shaft comprises a material selected from the group consisting of a metal claim 1 , a doped semiconductor claim 1 , and a conductive polymer.4. The system of claim 1 , wherein the shaft comprises a material selected from the group consisting of carbide nanowires claim 1 , carbon nanotubes claim 1 , and combinations thereof.5. The system of claim 1 , wherein the ...

DETECTION OF LOW CONCENTRATION BIOLOGICAL AGENTS

Provided are methods of preparing a sample for detection by placing the sample on a shrinkable scaffold and then shrinking the scaffold. An exemplary shrinkable scaffold is a thermoplastic substrate. 1. A method for preparing a sample for detection of a fluorescently labeled polynucleotide suspected to be present in the sample by detecting the amount of fluorescence emitted by the fluorescently labeled polynucleotide , comprising:covalently attaching the polynucleotide on a silica surface of a thermoplastic material, the silica surface having been deposited by e-beam vaporization or sputtering,heating and shrinking the thermoplastic material thereby concentrating the sample on the silica surface and to provide a rough porous substrate that results in an optical effect of light scattering.2. The method of claim 1 , wherein the material is pre-stressed prior to the shrinking and the shrinking comprises removing the stress.3. The method of claim 1 , wherein the shrinking is uniaxial or biaxial.4. The method of claim 1 , wherein the material is shrunk by at least 60%.5. The method of claim 1 , wherein the thermoplastic material comprises a high molecular weight polymer claim 1 , polyolefin claim 1 , a shape memory polymer claim 1 , polyethylene claim 1 , acrylonitrile butadiene styrene (ABS) claim 1 , acrylic claim 1 , celluloid claim 1 , cellulose acetate claim 1 , ethylene-vinyl acetate (EVA) claim 1 , ethylene vinyl alcohol (EVAL) claim 1 , fluoroplastics (PTFEs claim 1 , including FEP claim 1 , PFA claim 1 , CTFE claim 1 , ECTFE claim 1 , ETFE) claim 1 , ionomers kydex claim 1 , a trademarked acrylic/PVC alloy claim 1 , liquid crystal polymer (LCP) claim 1 , polyacetal (POM or Acetal) claim 1 , polyacrylates (Acrylic) claim 1 , polyacrylonitrile (PAN or Acrylonitrile) claim 1 , polyamide (PA or Nylon) claim 1 , polyamide-imide (PAI) claim 1 , polyaryletherketone (PAEK or Ketone) claim 1 , polybutadiene (PBD) claim 1 , polybutylene (PB) claim 1 , polybutylene ...

METHOD FOR SYNTHESISING AMBROXIDE FROM AGERATINA JOCOTEPECANA

The present invention is related to a process for obtaining the (−)-13,14,15,16-tetranor-8a,12-labdanediol compound from the plant, the process comprises the steps of a) obtaining an organic concentrated extract from the shoot system; b) subjecting the organic concentrated extract to column chromatography in order to elute a fraction with the (−)-13,14,15,16-tetranor-8a,12-labdanediol compound; c) separating the eluted fractions comprising the (−)-13,14,15,16-tetranor-8a,12-labdanediol compound; and d) evaporating the organic solvent to yield the (−)-13,14,15,16-tetranor-8a,12-labdanediol compound in a solid form. 1Ageratina jocotepecanaAgeratina jocotepecana. A process for obtaining the (−)-13 ,14 ,15 ,16-tetranor-8a ,12-labdanediol compound from the plant , wherein the process comprises the steps of: a) obtaining an organic concentrated extract from the shoot system; b) subjecting the organic concentrated extract to column chromatography to elute a fraction with the (−)-13 ,14 ,15 ,16-tetranor-8a ,12-labdanediol compound; c) separating the eluted fractions comprising the (−)-13 ,14 ,15 ,16-tetranor-8a ,12-labdanediol compound; and d) evaporating the organic solvent to yield the (−)-13 ,14 ,15 ,16-tetranor-8a ,12-labdanediol compound in a solid form.2Ageratina jocotepecanaAgeratina jocotepecanaAgeratina jocotepecanaAgeratina jocotepecanaAgeratina jocotepecanaAgeratina jocotepecana. The process according to claim 1 , wherein the organic concentrated extract from the shoot system is obtained by a process comprising the following steps: a) drying the plant shoot system claim 1 , wherein the shoot system comprises the aerial parts of the plant claim 1 , such as flowers claim 1 , leaves and/or stems; b) macerating or refluxing the plant shoot system in an organic solvent for a period of time from 6 to 72 hours; c) filtering out the macerated or refluxed extract of step (b) to obtain an organic extract; and d) evaporating the solvent from the organic extract.3. The ...

PREPARATION OF CHROMATOGRAPHIC STATIONARY PHASE HAVING POROUS FRAMEWORK MATERIAL AS MATRIX FOR CHIRAL SEPARATION

The novel porous framework materials (such as metal organic frameworks or covalent organic frameworks) having a wide range of applications, which was designed and developed in an inventive manner to resolve issues with respect to a carrier material in a stationary phase of a conventional chiral chromatographic column in which the carrier material has poor stability, a chiral resolving agent has a low loading rate, and the chiral resolving agent is prone to loss or is applied in a restricted manner. The porous framework material efficiently loads a chiral resolving agent (such as proteins, enzymes, or macrocyclic antibiotics) by means of covalent bonding, adsorption, embedding, and crosslinking, such that a variety of efficient and durable chiral stationary phases are prepared to serve as a novel high-performance chromatographic column filler used for chromatographic chiral separation (such as high-performance liquid chromatography or capillary chromatography). The various chiral stationary phases prepared by applying the above technique have high separation efficiency, high stability, and durability, and have been successfully applied to perform efficient separation of different kinds of chiral materials such as chiral amino acids and a chiral drug. The technique greatly widens the application range and extends the service life of a chiral chromatographic separation column. 18-. (canceled)9. A chiral stationary phase , comprising porous framework materials and biomolecules , wherein:the porous framework materials include at least one of metal-organic framework materials (MOFs), covalent organic framework materials (COFs) and hydrogen-bonded organic framework materials (HOFs);the biomolecules are biological chiral resolving agents;a pore size of the porous framework materials is 0.2-15 nm;the porous framework materials serve as solid carrier; andthe biomolecules are loaded into the porous framework materials.10. The chiral stationary phase in claim 9 , wherein the ...

APPLICATION OF MACROPOROUS SILICA SYNTHESIZED BY A SALT-TEMPLATED AEROSOL METHOD FOR CHROMATOGRAPHY

The present invention discloses a silica particle having a diameter less than or equal to 2 μη, wherein the particle is spherical and comprises interconnected pores having a diameter in the range from 50 nm to 300 nm. The silica particle is preferably produced by spray pyrolysis (=spray drying) of a silica colloid. In the production process, porosity is introduced by means of an inorganic salt, such as NaCl, KCI, LiCl, NaNO3 or Ll NO3, which serves as a pore template. The silica particle may further be functionalized with proteins, peptides, nucleic acids, polysaccharides and proteoglycans, preferably concanavalin A or avidin. The present invention further discloses the use of the silica particle in chromatography, in particular in affinity chromatography. 1. A silica particle having a diameter less than or equal to about 2 μm , wherein the particle is spherical and comprises interconnected pores having a diameter in the range from about 50 nm to about 300 nm.2. The silica particle of claim 1 , wherein the silica particle provides a support with a surface area of about 150 m/g to about 300 m/g.3. The silica particle of claim 1 , wherein the silica particle is functionalized with a stationary phase.4. The silica particle of claim 3 , wherein the stationary phase is selected from the group consisting of proteins claim 3 , peptides claim 3 , nucleic acids claim 3 , polysaccharides claim 3 , and proteoglycans.5. The silica particle of claim 4 , wherein the proteins are concanavalin A or avidin.6. The silica particle of claim 1 , wherein the silica particle is synthesized by spray pyrolysis of silica colloids.7. The silica particle of claim 6 , wherein porosity is introduced into the particle by means of an inorganic salt acting as a pore template.8. The silica particle of claim 7 , wherein the inorganic salt is selected from the group consisting of NaCl claim 7 , KCl claim 7 , LiCl claim 7 , NaNO claim 7 , LiNO claim 7 , combinations thereof claim 7 , and mixtures ...

Magnetic Separation

Devices, systems and methods for magnetically separating paramagnetic beads for biomolecule isolation and processing are disclosed. 122-. (canceled)23. A method comprising:drawing a liquid into a liquid conduit defining a flow axis; andmoving at least two magnets associated with the liquid conduit and spaced apart from one another in a direction parallel to the flow axis from a first position in which the magnets are separated from the associated liquid conduit in a direction perpendicular to the flow axis to a second position in which the magnets are juxtaposed to the associated liquid conduit.24. The method according to claim 23 , wherein liquid conduit comprises a capillary tube.25. The method according to claim 24 , wherein the liquid is drawn into the liquid conduit by capillary action.26. The method according to claim 23 , wherein the liquid is drawn into the liquid conduit by aspiration.27. The method according to claim 23 , wherein the method the method further comprises flowing liquid in the liquid conduit past the magnets in the first position.28. The method according to claim 23 , wherein the liquid comprises magnetic beads.29. The method according to claim 28 , wherein the magnetic beads comprise target biomolecules bound thereto.30. The method according to claim 29 , wherein the target biomolecules comprise DNA.31. The method according to claim 23 , wherein the method is performed in a system comprising:a plurality of liquid conduits, each liquid conduit defining a flow axis;for each liquid conduit, at least two magnets associated with the liquid conduit, the at least two magnets spaced apart from one another in a direction parallel to the flow axis defined by the associated liquid conduit; andat least one actuator associated with the magnets, the at least one actuator being capable of causing each magnet to assume either a first position in which the magnet is separated from the associated liquid conduit in a direction perpendicular to the flow axis ...

Novel Liquid Chromatographic Media and Methods of Synthesizing the Same

The present invention provides a bisamide-containing novel liquid chromatographic media and method of synthesizing the same. A novel polar bisamide functional group, which can form hydrogen bonds or ion pairs with residual silanols on the surface of silica gel, is used as the bonded phase on the surface of silica gel to better shield the activity of silanols and to eliminate the influence of residual silanol groups. Compared with conventional C18 columns, these novel bonded phases have different selectivity; they can work not only in 0 to 100% water but also in 0 to 100% organic mobile phase. In particular, they exhibit good peak shapes and resolutions for polar and basic compounds and have good stability within a very wide pH range. These properties make the new stationary phases a useful complement to conventional C18 columns for a variety of HPLC applications. 2. The liquid chromatographic media of claim 1 , wherein the silica gel substrate is a spherical porous silica gel with a particle size of 1 μm to 60 μm claim 1 , a pore size of 50 Å to 1000 Å claim 1 , and a specific surface area of 50 m/g to 500 m/g.3. The liquid chromatographic media of claim 1 , wherein the polar silane having two amide linkages has the general formula of{'br': None, 'sup': 1', '2, 'sub': 2', 'γ', '2', 'β', 'α', '3-α, 'RCONH(CH)CONH(CH)SiRX'}{'sup': '1', 'sub': 1', '20, 'wherein Ris substituted or unsubstituted C-Calkyl, phenyl, aralkyl, cycloalkyl, or heterocycloalkyl;'}{'sup': '2', 'sub': 1', '8, 'Ris substituted or unsubstituted C-Calkyl, phenyl, aralkyl, cycloalkyl, or heterocycloalkyl;'}α is 0, 1, or 2;β is an integral of 1 to 10;γ is an integer of 1 to 20; andX is halogen, alkoxy, acyloxy, or amino.4. The liquid chromatographic media of claim 1 , wherein the endcapping reagent is one or more selected from the group consisting of monosilane claim 1 , disilane claim 1 , trisilane claim 1 , tetrasilane claim 1 , and pentasilane.5. The liquid chromatographic media of claim 4 , wherein ...

Affinity Chromatography

This invention relates to a method of removing a chemical entity from a liquid using affinity chromatography. The method involves passing an elongate solid phase through a conduit through which the liquid also flows. 1. A method comprising: wherein the liquid from which the chemical entity is removed passes along the conduit from the liquid input port to the liquid output port in the opposite direction to the elongate body;', 'wherein the conduit being is configured such that the liquid contacts the elongate body; and', 'wherein attached to the elongate body is an affinity entity having an affinity for the chemical entity; and, 'passing an elongate body comprising a liquid input port and a liquid outlet port through a conduit to remove a chemical entity from a liquid;'}washing the elongate body to remove products present having lower affinity for the affinity entity than the chemical entity.2. The method of claim 1 , wherein one or both the elongate body and the liquid is subjected to sonication as it passes through the conduit.3. The method of claim 1 , wherein washing the elongate body comprises passing the elongate body through a wash conduit to remove the products present on the elongate body having lower affinity for the affinity entity than the chemical entity;wherein the wash conduit comprises a wash liquid input port and a wash liquid outlet port;wherein a wash liquid passes along the wash conduit from the wash liquid input port to the wash liquid output port in the opposite direction to the elongate body; andwherein the wash conduit is configured such that the wash liquid contacts the elongate body.4. The method of claim 3 , wherein one or both the elongate body and the wash liquid is subjected to sonication as it passes through the wash conduit.5. The method of further comprising recovering the chemical entity from the elongate body.6. The method of claim 5 , wherein recovering the chemical entity comprises passing the elongate body through a displacement ...

DIGITAL BIOSENSOR

The presently disclosed subject matter relates to a biodetection system centered on the development and optimization of a logistically simple assay for detecting, identifying, and/or quantifying microbial pathogens using an unmodified substrate. Specifically, the disclosed system quantitatively measures target analytes (e.g., bacteria) isolated over a digitally-encoded substrate. Isolated microbes are positioned in specific locations and geometries on the substrate data surface, resulting in a discernible interruption and/or change to data being read from the substrate. The change can be exploited to indicate positive detection and detection counts for one or more specific microbes. 1. A digital biosensor for detecting the presence or amount of one or more analytes in a sample , the digital biosensor comprising:an optically read digital substrate that includes a top face with a layer comprising a data path capable of being read by an optical drive incident upon the layer, wherein the data path is encoded with a baseline data that is static, and wherein the top face is defined by an assay surface used for sample deposition;a cover overlaid on the top face of the digital substrate, wherein the cover is attached to the top face about the perimeter of the digital substrate;wherein the digital biosensor is configured such that an amount of the one or more analytes can be positioned between the top face of the digital substrate and the cover; andwherein the presence, amount, or both of the one or more analytes can be detected by an interruption or change to the baseline data being read from the digital substrate by a digital optical device.2. The digital biosensor of claim 1 , wherein the digital substrate is a digital optical disk.3. The digital biosensor of claim 1 , wherein the analyte is selected from the bacteria claim 1 , viruses claim 1 , fungi claim 1 , spores claim 1 , or combinations thereof.4. The digital biosensor of claim 1 , wherein the digital substrate is ...

METHOD FOR PRODUCING POROUS CELLULOSE MEDIUM

Provided are a technique for preparing a porous cellulose medium without using a special gelling agent for a solution in which cellulose acetate serving as a raw material is dissolved; and a porous cellulose medium and the like produced using the technique. A method for producing a porous cellulose medium comprises the step of preparing a flowable homogeneous composition comprising cellulose acetate, a basic compound, and a solvent including water, and gelling the composition by deacetylation reaction of the cellulose acetate. 1. A method for producing a porous cellulose medium comprising the step of preparing a flowable homogeneous composition comprising cellulose acetate , a basic compound , and a solvent including water , and gelling the composition by deacetylation reaction of the cellulose acetate.2. A method for producing spherical porous cellulose particles comprising the step of obtaining a dispersion by dispersing a flowable homogeneous composition comprising cellulose acetate , a basic compound , and a solvent including water in a dispersion medium not miscible with the homogeneous composition , and forming gelled particles composed of the composition by gelling the composition by deacetylation reaction of the cellulose acetate contained in the obtained dispersion.3. The method for producing spherical porous cellulose particles according to claim 2 , which comprises claim 2 , after the preceding step claim 2 , the step of adding a separating solvent for separating the obtained gelled particles to the dispersion in which the gelled particles are formed claim 2 , thereby separating the gelled particles into the separating solvent.4. The method according to claim 3 , wherein the separating solvent is water claim 3 , methanol claim 3 , ethanol claim 3 , 2-propanol claim 3 , acetamide claim 3 , formamide claim 3 , or a mixture of at least two of these solvents.5. A method for producing a porous cellulose monolith comprising the step of placing a flowable ...

SAMPLE SEPARATION DEVICE BASED ON PAPER FOLDING

The present exemplary embodiments provide a sample separation device which applies an electric field to a selective ion permeable layer based on origami to concentrate a target material in a specific area and concentrates a target material and separates a non-target material through a filter layer in which a paper is compressed to adjust a size of micro pore. 1. A sample separation device , comprising:a base which includes a plurality of base units;a coating layer which is located in at least a partial area of the base to prevent the adsorption of a sample and separate a storage space or a movement path;a plurality of reservoirs which has an area set by the collating layer, is located in the plurality of base units, and stores or moves a collection object to be separated from the sample or a separation object excluding the collection object included in the sample; anda selective ion permeable layer which is coupled to some reservoirs of the plurality of reservoirs to selectively transmit ions.2. The sample separation device of claim 1 , wherein a first end base unit located at one end of the base includes a first end reservoir as the reservoir and a second end base unit located at the other end of the base includes a second end reservoir as the reservoir and the first end reservoir and the second end reservoir are connected to the selective ion permeable layer.3. The sample separation device of claim 2 , wherein the selective ion permeable layer is located in a third end reservoir which is the reservoir located in a third end base unit adjacent to the first end base and a fourth end reservoir which is the reservoir located in a fourth end base unit adjacent to the second end base unit.4. The sample separation device of claim 1 , further comprising:an injection base unit which is directly connected to a base unit which is not located at the end among the plurality of base units and at least partially located at the outside when the base is folded, and injects the ...

Separation Method

The invention relates to a method of isolating an immunoglobulin, comprising the steps of: 1. A method of isolating an immunoglobulin , comprising the steps of:a) providing a separation matrix comprising at least 15 mg/ml multimers of immunoglobulin-binding alkali-stabilized Protein A domains covalently coupled to a porous support, wherein said porous support comprises cross-linked polymer particles having a volume-weighted median diameter (d50,v) of 56-70 micrometers and a dry solids weight of 55-80 mg/ml;b) contacting a liquid sample comprising an immunoglobulin with said separation matrix;c) washing said separation matrix with a washing liquid;d) eluting the immunoglobulin from the separation matrix with an elution liquid; ande) cleaning the separation matrix with a cleaning liquid comprising at least 0.5 M NaOH.2. The method of claim 1 , wherein said cross-linked polymer particles have a pore size corresponding to an inverse gel filtration chromatography Kd value of 0.69-0.85 for dextran of Mw 110 kDa.3. The method of claim 1 , wherein said separation matrix has a max pressure of at least 0.58 claim 1 , such as at least 0.60 claim 1 , MPa when packed at 300 +/−10 mm bed height in a FineLine™ 35 column.4. The method of claim 1 , wherein said immunoglobulin comprises an Fc fusion protein.5. The method of claim 1 , wherein said immunoglobulin has a hydrodynamic radius of at least 6.0 nm.6. The method of claim 1 , wherein said immunoglobulin comprises a bispecific claim 1 , trispecific or polyspecific antibody.7. The method of claim 6 , wherein said method separates half antibodies or homodimeric antibodies from said bispecific claim 6 , trispecific or polyspecific antibody.8. The method of claim 1 , wherein said immunoglobulin comprises a conjugated antibody.9. The method of claim 1 , wherein said immunoglobulin comprises an antibody fragment.10. The method of claim 1 , wherein the separation matrix is Mabselect™ PrismA.11. A method of isolating an immunoglobulin ...

SENSOR ARRANGEMENT FOR MEASURING THE CONCENTRATION OF A SUBSTANCE

A sensor arrangement for determining a concentration of a substance in an open sample in the presence of an interfering material is disclosed. The sensor arrangement comprises a first light source emitting pulsed light at a first wavelength being absorbed by said substance, a second light source emitting pulsed light at a second wavelength being transmitted through said substance, optical means for directing at least a part of the emitted pulsed light of said first and second wavelengths through the open sample along the same optical path, and a sample detector arranged at an end of the optical path for receiving the emitted light of said first and second wavelengths being transmitted through the sample. The interfering material is formed as deposits on at least one of said optical means being exposed to said substance, and said first wavelength and said second wavelength are absorbed by said interfering material. 1. A sensor arrangement for determining a concentration of a substance in an open sample in the presence of an interfering material , comprising:a first light source emitting pulsed light at a first wavelength being absorbed by said substance,a second light source emitting pulsed light at a second wavelength being transmitted through said substance,optical means for directing at least a part of the emitted pulsed light of said first and second wavelengths through the open sample along the same optical path, anda sample detector arranged at an end of the optical path for receiving the emitted light of said first and second wavelengths being transmitted through the sample,wherein said interfering material is formed as deposits on at least one of said optical means being exposed to said substance, and wherein said first wavelength and said second wavelength are absorbed by said interfering material.2. The sensor arrangement of claim 1 , wherein the first light source claim 1 , the second light source claim 1 , and the sample detector are arranged on the same ...

Method For Preparing Natural Organic Macromolecular Water Treatment Agent

Method for preparing a natural organic macromolecular water treatment agent including: dissolving amylose corn starch in an alkali solution, stirring for 30 min, to obtain a suspension, freezing the suspension to fully frozen state, melting and dialyzing, to obtain a corn starch dispersion; mixing a modified flax fiber, the dispersion, nano-hybrid silica and distilled water, performing 800 W ultrasonication for 10 min, to obtain a treated suspension; taking an amount of a superabsorbent macromolecular resin with a certain shape, making it absorb water and swell into a solid hydrogel with the certain shape; mixing the solid hydrogel and the treated suspension, static defoaming, loading into a mold and solidifying, drying until the solid hydrogel is completely dehydrated, to obtain a hollow agent; spraying a catalytic degrading agent/toxin degrading agent on the surface of the hollow agent and/or the inner wall of holes thereof, to obtain the target agent.

HYDROPHOBIC INTERACTION CHROMATOGRAPHY CARRIER AND PROTEIN PURIFICATION METHOD

A chromatography carrier capable of removing an antibody dimer from a solution containing an antibody monomer. The chromatography carrier includes a base carrier containing porous particles and a hydrophobic ligand bound to the base carrier, and has an electric conductivity of 34 mS/cm or less measured by a gradient elution test. The porous particles preferably have an average particle diameter of 66 to 150 μm, and the hydrophobic ligand preferably has at least one selected from a group consisting of phenyl, n-butyl, n-hexyl, n-octyl, and n-octadecyl.

CATION EXCHANGE CHROMATOGRAPHY WASH BUFFER

A wash buffer comprising a surfactant for use in affinity and cation exchange chromatography to purify proteins of interest from protein aggregates and to remove and/or inactivate viruses. When used during affinity or cation exchange chromatography for the purification of a protein of interest, such as an antibody, the wash buffer significantly improves viral clearance from the preparation, while also reducing the levels of host cell proteins and protein aggregates. Following affinity or cation exchange chromatography with the wash buffer, the protein of interest may be further purified using other chromatography and filtration operations. 1. A method of purifying a protein of interest bound to a cation exchange (CEX) chromatography support comprising applying a wash solution comprising one or more surfactants to the CEX chromatography support.2. (canceled)3. The method of claim 1 , comprising loading a mixture comprising a protein of interest and one or more contaminant proteins claim 1 , aggregates claim 1 , and/or viruses onto the CEX chromatography support before applying the wash solution comprising one or more surfactants to the CEX chromatography support claim 1 , and then eluting the protein of interest from the support claim 1 , thereby forming a purified eluate of the protein of interest.4. A method for purifying a protein of interest claim 1 , comprising loading a mixture comprising a protein of interest and one or more contaminant proteins claim 1 , aggregates claim 1 , and/or viruses onto a CEX chromatography support claim 1 , washing the support with an aqueous wash solution comprising one or more surfactants to elute the one or more contaminant proteins claim 1 , aggregates claim 1 , and/or viruses from the support claim 1 , and then eluting the protein of interest from the support claim 1 , thereby forming a purified eluate of the protein of interest.5. The method of claim 3 , wherein one or more surfactants comprises Lauryldimethylamine N-oxide ( ...

POROUS CELLULOSE GEL, METHOD FOR PRODUCING THE SAME, AND USE THEREOF

The present invention provides a porous cellulose gel having a high mechanical strength capable of being operated at a higher flow rate, and a method for producing the same. To a suspension liquid of cellulose particles, a crosslinking agent in an amount of from 4 to 12 times the amount of the cellulose monomer in terms of moles and an alkali in an amount of from 0.1 to 1.5 times the amount of the crosslinking agent in terms of moles are added continuously dropwise or added dividedly over a prescribed period of time, whereby flow rate characteristics of a resulting porous cellulose gel can be enhanced. According to the invention, the production efficiency of a polymer substance, such as a nucleic acid and a protein can be enhanced. 1. A porous cellulose gel comprising crosslinked cellulose particles having a solvent contained therein , upon filling the gel in a chromatography column having an inner diameter of 2.2 cm to a height of 17.5±2.5 cm , a linear velocity of water at 20° C. being from 2 ,400 to 4 ,500 cm/hour at a pressure of 0.4 MPa.2. A porous cellulose gel comprising crosslinked cellulose particles having a solvent contained therein , upon filling the gel in a chromatography column having an inner diameter of 2.2 cm to a height of 17.5±2.5 cm , a maximum linear velocity of water at 20° C. being from 2 ,400 to 5 ,500 cm/hour.3. The porous cellulose gel of claim 1 , wherein the crosslinked cellulose particles have a particle diameter of from 1 to 2 claim 1 ,000 μm.4. The porous cellulose gel of claim 1 , wherein the porous cellulose gel has an exclusion limit molecular weight with polyethylene glycol of from 1 claim 1 ,000 claim 1 ,000 to 5 claim 1 ,000 claim 1 ,000.5. The porous cellulose gel of claim 1 , wherein the crosslinked cellulose particles have a swelling degree of from 5 to 20 mL/g.6. The porous cellulose gel of claim 1 , wherein the crosslinked cellulose particles have a reswelling degree of from 80 to 100%.711-. (canceled)12. A filler for ...

ABSORBENT/SOLUBILIZING MATERIALS BASED ON MICROPOROUS ORGANOGELS

The present invention relates to the use of a microporous organogel for the capture of fluids by adsorption and/or for the controlled release of fluids after solubilization. The fluids are, in particular, air or water pollutants or volatile substances. The invention also relates to the use of a microporous organogel in a process for analyzing the captured fluids. The invention also relates to any fluid sensor by moulding of a microporous organa gel. 1. A microporous organogel capable of being used for trapping of one or more fluids by adsorption and/or for controlled release of one or more fluids after solubilization.2. An organogel according to claim 1 , wherein the one or more fluids are concentrated in the microporous organogel.3. An organogel according to claim 1 , wherein the one or more fluids comprise one or more organic compounds.4. An organogel according to claim 1 , wherein the one or more fluids comprises one or more air or water polluting agents claim 1 , optionally one or more hydrocarbons claim 1 , and/or heavy metals.5. An organogel according to claim 1 , wherein the one or more fluids are volatile substances claim 1 , optionally comprising one or more gases or vapors claim 1 , optionally one or more pheromones of insects claim 1 , aromas of plants claim 1 , flowers or resins.6. An organogel according to claim 1 , wherein the microporous organogel is hydrophobic.7. An organogel according to claim 1 , wherein the microporous organogel has a porosity of 10% to 90% claim 1 , optionally from 55% to 70%.8. An organogel according to claim 1 , wherein the mean diameter of the pores of the microporous organogel is 0.5 μm to 550 μm claim 1 , optionally 220 μm.9. An organogel according to claim 1 , wherein the microporous organogel is obtained using a method comprising preparing the organogel and forming pores in said organogel claim 1 , optionally by aqueous dissolution of a template of one or more solid water-soluble particles of calibrated size previously ...

AMYLOSE DERIVATIVE AND OPTICAL ISOMER SEPARATING AGENT CONTAINING SAME

Provided are a novel amylose derivative which exhibits excellent optical isomer separability and which is suitable as an optical isomer separating agent; and an optical isomer separating agent containing the amylose derivative. A task is attained by an amylose derivative having a constituent unit represented by formula (I) below; In below formula (I), Ris a substituent group represented by any of formulae 1 to 3 below, and Ris a substituent group represented by any of formulae a to g below. Rand Rare different substituent groups. A combination of Rand Rin which Ris a substituent group represented by structural formula 3 and Ris a substituent group represented by structural formula c is excluded from the formula (I). 2. The amylose derivative according to claim 1 , wherein Ris a substituent group represented by the structural formula 1 and Ris a substituent group represented by any of the structural formulae a claim 1 , b claim 1 , e and f in the constituent unit represented by the formula (I).3. The amylose derivative according to claim 1 , wherein Ris a substituent group represented by the structural formula 2 and Ris a substituent group represented by any of the structural formulae a claim 1 , c claim 1 , d claim 1 , e and g in the constituent unit represented by the formula (I).4. The amylose derivative according to claim 1 , wherein Ris a substituent group represented by the structural formula 3 and Ris a substituent group represented by any of the structural formulae a claim 1 , b claim 1 , d claim 1 , e and f in the constituent unit represented by the formula (I).5. An optical isomer separating agent claim 1 , which is constituted from a carrier and the amylose derivative according to which is carried by the carrier.7. The method for producing an amylose derivative according to claim 6 , wherein Ris a substituent group represented by structural formula 1 in a first substituent group represented by the formula (II-1) and Ris a substituent group represented by ...

Separation Matrix and Method of Separation

The invention discloses a separation matrix comprising polysaccharide gel beads, wherein said polysaccharide gel beads comprise embedded fibers. The invention further discloses a method of preparing the separation matrix and use of the matrix for separation purposes. 1. A separation matrix comprising polysaccharide gel beads , wherein said polysaccharide gel beads comprise embedded fibers.2. The separation matrix of claim 1 , wherein said embedded fibers are dispersed in the polysaccharide gel beads.3. The separation matrix of claim 1 , wherein the embedded fibers are chemically cross-linked to the polysaccharide.4. The separation matrix of claim 1 , wherein the separation matrix comprises embedded fibers of up to 80% of the non-fiber matrix dry weight.5. The separation matrix of claim 1 , wherein the embedded fibers comprise cellulose or a cellulose derivative.6. The separation matrix of claim 1 , wherein the embedded fibers comprise microfibrillated cellulose.7. The separation matrix of claim 1 , wherein the embedded fibers are furcated.8. The separation matrix of claim 1 , wherein the polysaccharide gel beads comprise agarose or agar.9. The separation matrix of claim 1 , comprising polysaccharide gel beads with sphericity greater than 0.95.10. A method for preparing a separation matrix claim 1 , which method comprises the steps ofa. mixing an aqueous solution of at least one gellable polysaccharide with fibersb. forming gel beads of said aqueous solution.11. The method of claim 10 , further comprising claim 10 , after step b. claim 10 , a step c. of cross-linking said gellable polysaccharide of said gel beads.12. The method of claim 10 , further comprising claim 10 , after step b. claim 10 , a step c. of cross-linking said gellable polysaccharide with said fibers.13. The method according to claim 10 , further comprising a step of attaching chromatography ligands to reactive hydroxyl groups of said gel beads.14. The method according to claim 10 , wherein the ...

MATERIALS FOR HYDROPHILIC INTERACTION CHROMATOGRAPHY AND PROCESSES FOR PREPARATION AND USE THEREOF FOR ANALYSIS OF GLYCOPROTEINS AND GLYCOPEPTIDES

The invention relates to poly-amide bonded hydrophilic interaction chromatography (HILIC) stationary phases and novel HILIC methods for use in the characterization of large biological molecules modified with polar groups, known to those skilled in the art as glycans. The invention particularly provides novel, poly-amide bonded materials designed for efficient separation of large biomolecules, e.g. materials having a large percentage of larger pores (i.e. wide pores). Furthermore, the invention advantageously provides novel HILIC methods that can be used in combination with the stationary phase materials described herein to effectively separate protein and peptide glycoforms by eliminating previously unsolved problems, such as on-column aggregation of protein samples, low sensitivity of chromatographic detection of the glycan moieties, and low resolution of peaks due to restricted pore diffusion and long intra/inter-particle diffusion distances. 199-. (canceled)100. A porous material comprising a copolymer comprising at least one hydrophilic monomer and a first poly-amide bonded phase and a second poly-amide bonded phase , wherein:the average pore diameter is greater than or equal to about 200 Å; and a porous or nonporous organic-inorganic hybrid core comprising an aliphatic bridged silane,', 'at least one hydrophilic monomer grafted to the core, and', 'the first and the second poly-amide bonded phases are bonded to the at least one hydrophilic monomer., 'the porous material comprises101. The porous material of claim 100 , wherein the porous material comprises a porous particle that comprises said copolymer.102. The porous material of claim 100 , wherein the porous material comprises a porous monolith that comprises said copolymer.104. The porous material of claim 100 , wherein the first poly-amide bonded phase is derived from acrylamide.105. The porous material of claim 100 , wherein the second poly-amide bonded phase is derived from N claim 100 ,N- ...

MATERIALS AND METHODS FOR THE DETECTION OF TRACE AMOUNTS OF SUBSTANCES IN BIOLOGICAL AND ENVIRONMENTAL SAMPLES

The subject invention provides chemical compositions and synthesis strategies to create molecularly imprinted polymers (MIPs) via sol-gel processes. In a specific embodiment, the subject invention utilizes a(n) organic, inorganic, or metallic template analyte to create a hybrid organic-inorganic or inorganic three-dimensional network possessing cavities complementary to the shape, size, and functional orientation of the template molecule or ions. The subject invention further pertains to the use of the novel MIPs as selective solid phase extraction (SPE) sorbents for pre-concentration and clean-up of trace substances in biological and environmental samples. Synthesis of other molecularly imprinted polymers with environmental, pharmaceutical, chemical, clinical, toxicological, and national security implications can be conducted in accordance with the teachings of the subject invention. 1. A method of synthesizing a molecularly imprinted polymer (MIP) matrix , comprising:mixing a target analyte with a sol-gel precursor and incubating the mixture to form a polymer network;hydrolyzing a cross-linking agent with a hydrolytic agent in the presence of a reaction catalyst;adding the hydrolyzed cross-linking agent to the polymer network and allowing the network to age and ripen; andextracting the target analyte using a solvent, leaving behind molecular cavities in the polymer network that are complementary in size, shape, and functionality to the target analyte.2. The method according to claim 1 , characterized in that the target analyte is selected from drugs claim 1 , cells claim 1 , proteins claim 1 , amino acids claim 1 , toxins claim 1 , and viruses.3. The method according to claim 2 , characterized in that the target analyte is an antibiotic drug selected from chloramphenicol claim 2 , thiamphnicol claim 2 , florfenicol claim 2 , ceftiofur claim 2 , cefaclor claim 2 , oxytetracycline claim 2 , tetracycline claim 2 , sulfamethazine claim 2 , sulfadimethoxine claim 2 , ...

CHROMATOGRAPHY COLUMN WITH LOCKED PACKED BED AND METHOD OF PACKING THAT COLUMN

A chromatography column has a retaining plug permanently fixed to an upstream end of the column and blocks one end of the bore through the column. The plug has a fluid passage therethrough. An upstream end of the passage is preferably but optionally larger in diameter than a downstream end of the passage. An upstream porous member upstream of the retaining plug is held by an upstream end cap and urged toward the plug. Chromatographic media extends from the upstream porous member, through the passage in the retaining plug, to a downstream porous member held by a downstream end cap. The media between the retaining plug and the downstream porous member are under compression to form a bed of packed media. 1. A chromatography column having a tubular body with opposing upstream and downstream ends with upstream and downstream end fittings connected to the tubular body , the tubular body having an internal bore extending along a longitudinal axis of the tubular body and column , the column comprising:a retaining plug blocking an upstream end of the bore and having a passage extending between upstream and downstream ends of the retaining plug and filled with chromatographic media;a downstream porous member blocking the downstream end of the bore, the bore having a continuous wall between the retaining plug and the downstream porous member;a slurry-packed bed of chromatographic media held in compression in the bore by the retaining plug and the downstream porous member;2. The column of claim 1 , wherein the passage contains porous media and further comprising an upstream porous member upstream of the retaining plug located to filter fluid flow through the bore into the at least one passage.3. The column of claim 2 , wherein the passage has a first diameter at the upstream end of the retaining plug and a second diameter at the downstream end of the retaining plug and the first diameter is larger than the second diameter.4. The column of claim 3 , wherein the first diameter is ...

Electro-enhanced solid-phase microextraction method

The electro-enhanced solid-phase microextraction (EE-SPME) method involves extraction that is performed with an SPME fiber, preferably made of polydimethylsiloxane (PDMS), in the presence of an applied electric potential for a predetermined period of time. Polar analytes are extracted onto the fiber, which is the stationary phase. The solid-phase microextraction fiber is then inserted into an injection port of a gas chromatograph coupled with a mass spectrometer. The polar analytes are desorbed in the injection port, and are analyzed by GC-MS to detect and quantify the individual components. The method is particularly useful for the detection of trace amounts of plasticizers, such as phthalate esters and bisphenol A, that are known to disrupt the endocrine system of humans and animals above known levels

ORGANIC GEL OR LIQUID CHROMATOGRAPHY METHOD

A chromatography method in which a gaseous, liquid or supercritical mobile phase containing species to be separated is circulated through a packing. The packing includes a plurality of capillary ducts extending in the packing between an upstream face through which the mobile phase enters the packing and a downstream face through which the mobile phase leaves the packing. A continuous medium permeable to molecular diffusion extends between the ducts, including a porous organic gel or an organic liquid with at least one network of connected pores, the size of which is greater than two times the molecular diameter of at least one species to be separated. The at least one species has a diffusive path between the ducts. 1. A packing for chromatography , comprising:a plurality of capillary conduits crossing the packing between an upstream face configured for inflowing of a mobile phase into the packing and a downstream face configured for an outflow of the mobile phase from the packing, anda continuous medium permeable to molecular diffusion extending between said conduits, including a porous organic gel or an organic liquid and including at least one network of connected pores.2. The packing of claim 1 , wherein the diameter of the capillary conduits of the packing is less than or equal to 500 μm claim 1 , preferably less than or equal to 150 μm claim 1 , and even preferably less than or equal to 80 μm.3. The packing of claim 1 , wherein the organic gel forming the continuous medium is selected from:(a) a copolymer of styrene and of divinylbenzene,(b) polymethyl methacrylate, and(c) a copolymer of hydroxyethyl methacrylate and of divinylbenzene.4. The packing of claim 1 , wherein said continuous medium comprises a polyholoside.5. The packing of claim 1 , wherein the organic liquid is selected from:(a) an aliphatic or aromatic hydrocarbon,(b) an aliphatic or aromatic alcohol,(c) an aliphatic or aromatic ketone,(d) an aliphatic or aromatic amine, and(e) a halogenated ...

PEPTOID AFFINITY LIGANDS

Compounds of Formulas I: 6. The compound of claim 1 , wherein:{'sup': '1', 'Ris a basic aromatic group; and/or'}{'sup': '5', 'Ris a basic aromatic group.'}7. The compound of claim 1 , wherein:{'sup': '1', 'Ris selected from the group consisting pyridinyl-, quinolinyl-, isoquinolinyl-, acridinyl-, pyrazinyl-, quinoxalinyl-, imidazolyl-, benzimidazolyl-, purinyl-, pyrazolyl-, indazolyl-, pyrimidinyl-, quinazolinyl-, pyridazinyl-, and cinnolinyl-; and/or'}{'sup': '2', 'Ris selected from the group consisting of phenyl-, benzyl-, naphtalyl-, indolyl-, and substituted derivatives thereof; and/or'}{'sup': '3', 'Ris selected from the group consisting of primary, secondary, tertiary and quaternary amine, guanidinyl-, carbamoyl-, hydroxyl-, and urea; and/or'}{'sup': '4', 'Ris H, and/or'}{'sup': '5', 'Ris an aromatic group, a basic group, or a basic aromatic group.'}8. The compound of claim 1 , wherein said solid support comprises a particle.9. The compound of claim 1 , wherein said solid support comprises an inorganic material.10. The compound of claim 1 , wherein said solid support comprises an organic polymer material.11. A method of binding an antibody or antibody Fc fragment from a liquid composition containing the same claim 1 , comprising the steps of{'claim-ref': {'@idref': 'CLM-00001', 'claim 1'}, '(a) providing a solid support comprising the compound of , wherein R is —OR′ or —NHR′;'}(b) contacting said composition to said solid support so that said antibody or Fc fragments bind to said compound; and(c) separating said liquid composition from said solid support, with said antibody or Fc fragment bound to said solid support.12. The method of claim 11 , wherein said liquid composition further comprises at least one proteolytic enzyme.13. The method of claim 11 , wherein said liquid composition comprises a biological fluid.14. The method of claim 13 , wherein said biological fluid comprises blood claim 13 , blood sera claim 13 , blood plasma claim 13 , tissue or cell ...

SYSTEMS AND METHODS FOR AUTOMATED REUSABLE PARALLEL BIOLOGICAL REACTIONS

A method comprises magnetically holding a bead carrying biological material (e.g., nucleic acid, which may be in the form of DNA fragments or amplified DNA) in a specific location of a substrate, and applying an electric field local to the bead to isolate the biological material or products or byproducts of reactions of the biological material. For example, the bead isolated from other beads having associated biological material. The electric field in various embodiments concentrates reagents for an amplification or sequencing reaction, and/or concentrates and isolates detectable reaction by-products. For example, by isolating nucleic acids around individual beads, the electric field can allow for clonal amplification, as an alternative to emulsion PCR. In other embodiments, the electric field isolates a nanosensor proximate to the bead, to facilitate detection of at least one of local pH change, local conductivity change, local charge concentration change and local heat. The beads may be trapped in the form of an array of localized magnetic field regions. 1. A system , comprising:(a) at least one electrode that applies an electric field that concentrates or isolates molecules to each of a plurality of particles, wherein said plurality of particles are coupled to biological species; and(b) a substantially planar sensor array adjacent to or downstream of said at least one electrode, wherein said substantially planar sensor array comprises a plurality of sensors that sense biological interaction events between said molecules and said biological species.2. The system of claim 1 , wherein said biological species are nucleic acid molecules and said biological interaction events are nucleic acid incorporation events claim 1 , and wherein in (b) claim 1 , said plurality of sensors sense said nucleic acid incorporation events during sequencing of said nucleic acid molecules.3. The system of claim 2 , further comprising a separation module upstream of said substantially ...

Polyimide precursor solution and polyimide shaped article

A polyimide precursor solution contains a polyimide precursor, wherein in the case where the polyimide precursor is analyzed by gel permeation chromatography, the elution curve of the polyimide precursor has a region A including a higher-molecular-weight peak and a region B including a lower-molecular-weight peak; a weight average molecular weight determined from the region A in terms of polystyrene is approximately 10,000 or more, and a weight average molecular weight determined from the region B in terms of polystyrene is approximately less than 10,000; and when the area of the region A is a and the area of the region B is b, the polyimide precursor satisfies Equation (1) a /( a+b )=approximately from 0.70 to 0.98.  Equation 1:

AFFINITY CHROMATOGRAPHY MATRIX

The invention discloses an immunoglobulin-binding protein comprising one or more mutated immunoglobulin-binding domains (monomers) of staphylococcal Protein A (E, D, A, B, C) or protein Z or a functional variant thereof, wherein in at least one of the one or more mutated monomers, the asparagine or histidine at the position corresponding to H18 of the B domain of Protein A or of Protein Z has been deleted or substituted with a first amino acid residue which is not proline or asparagine and wherein, if the amino acid residue at position 57 is proline and the amino acid residue at position 28 is asparagine, then the amino acid residue at the position corresponding to H18 of the B domain of protein A or of protein Z is not serine, threonine or lysine. 1. An immunoglobulin-binding protein comprising one or more mutated immunoglobulin-binding domains (monomers) of staphylococcal Protein A (E , D , A , B , C) or protein Z or a functional variant thereof , wherein in at least one of the one or more mutated monomers , the Asparagine or Histidine at the position corresponding to H18 of the B domain of Protein A or of Protein Z has been substituted with a first amino acid residue which is not Proline or Asparagine and wherein , if the amino acid residue at position 57 is Proline and the amino acid residue at position 28 is Asparagine , then the amino acid residue at the position corresponding to H18 of the B domain of Protein A or of Protein Z is not Serine , Threonine or Lysine.2. The protein of claim 1 , wherein additionally at least one of the Proline at position 57 and the Asparagine at position 28 has been substituted with a second amino acid residue.3. The protein of claim 1 , wherein neither of the amino acid residues at positions 57 and 28 is Proline or Asparagine.4. The protein of claim 1 , wherein the amino acid residue at position 18 is selected from the group consisting of Serine claim 1 , Glutamic acid claim 1 , Aspartic acid claim 1 , Threonine and Lysine.5. The ...

INTERSTITIAL FLUID CAPTURE DEVICE AND METHOD OF MEASURING ELECTROLYTE LEVELS USING SAME

Embodiments of the inventive concept include an interstitial fluid capture device configured to capture an interstitial fluid sample from the mouth of a user, an electrolyte measurement kit including said device, and a method for measuring electrolyte levels using said device. An exemplary device includes a main body portion configured for placement in the mouth of a user, the main body portion including a reservoir containing a hypertonic solution and having an opening that is sealed by a semipermeable membrane that permits a sample of interstitial fluid from the mouth of the user to be drawn through the membrane and into the solution reservoir. Various analyses may be performed on the captured interstitial fluid sample after removal of the device, including but not limited to, the assessment of one or more electrolyte levels through the use of various test strips or other techniques. 1. An interstitial fluid capture device , comprising:a main body portion configured for temporary retention in the mouth of a user;a solution reservoir located within the main body portion, the reservoir forming an opening along a fluid extraction side of the main body portion;a hypertonic solution residing in the solution reservoir of the main body portion; anda semipermeable membrane covering the solution reservoir opening and sealing the hypertonic solution within the solution reservoir, the semipermeable membrane constructed from a material that will permit interstitial fluid from mouth tissue of the user to be drawn through the semipermeable membrane and into the solution reservoir during use.2. The interstitial fluid capture device of claim 1 , wherein the main body portion is configured for temporary retention in a position selected from the group consisting of between the teeth and lip of the user claim 1 , between the teeth and cheek of the user claim 1 , and under the tongue of the user.3. The interstitial fluid capture device of claim 1 , wherein the main body portion is ...

SEPARATING AGENT FOR OPTICAL ISOMERS

Provided is a separating agent for optical isomers, which is excellent in solvent resistance and has optical separating ability comparable to or higher than that of existing separating agents for optical isomers of chemical bonding type or physical adsorption type. In the separating agent for optical isomers, amylose (3-chloro-5-methylphenylcarbamate) is supported on a carrier through chemical bonding. 1. A separating agent for optical isomers , comprising amylose (3-chloro-5-methylphenylcarbamate) and a carrier wherein amylose (3-chloro-5-methylphenylcarbamate) is supported on the carrier through chemical bonding.2. The separating agent for optical isomers according to claim 1 , wherein the separating agent for optical isomers is obtained by a method comprising a step of physically adsorbing amylose tris(3-chloro-5-methylphenylcarbamate) onto a carrier claim 1 , and a step of crosslinking the amylose tris(3-chloro-5-methylphenylcarbamate) and the carrier through irradiation of light energy.3. The separating agent for optical isomers according to claim 1 , wherein the separating agent for optical isomers is obtained by a method comprising a step of chemically bonding a reducing terminal of amylose and a carrier claim 1 , and a step of claim 1 , thereafter claim 1 , causing hydroxyl groups of amylose and 3-chloro-5-methylphenylisocyanate to react.4. The separating agent for optical isomers according to claim 1 , wherein the separating agent for optical isomers is obtained by a method comprising a step of copolymerizing a carrier having a polymerizable functional group claim 1 , amylose (3-chloro-5-methylphenylcarbamate) having a polymerizable functional group claim 1 , and a polymerizable monomer.5. The separating agent for optical isomers according to claim 1 , wherein the separating agent for optical isomers is obtained by a method comprising a step of modifying hydroxyl groups of amylose with 3-chloro-5-methylphenylisocyanate claim 1 , and thereafter obtaining an ...

Use of vapor deposition coated flow paths for improved chromatography of metal interacting analytes

R1, R2, R3, R4, R5, and R6 are each independently selected from (C1-C6)alkoxy, —NH(C1-C6)alkyl, —N((C1-C6)alkyl)2, OH, ORA, and halo. RA represents a point of attachment to the interior surfaces of the fluidic system. At least one of R1, R2, R3, R4, R5, and R6 is ORA. X is (C1-C20)alkyl, —O[(CH2)2O]1-20—, —(C1-C10)[NH(CO)NH(C1-C10)]1-20-, or —(C1-C10)[alkylphenyl(C1-C10)alkyl]1-20-.

HIGH PURITY CHROMATOGRAPHIC MATERIALS COMPRISING AN IONIZABLE MODIFIER

The present invention provides novel chromatographic materials, e.g., for chromatographic separations, processes for its preparation and separations devices containing the chromatographic material; separations devices, chromatographic columns and kits comprising the same; and methods for the preparation thereof. The chromatographic materials of the invention are high purity chromatographic materials comprising a chromatographic surface wherein the chromatographic surface comprises a hydrophobic surface group and one or more ionizable modifier. 117-. (canceled)19. The high purity chromatographic material of claim 18 , wherein the ratio of the hydrophobic surface group:ionizable modifier is from about 2.5:1 to about 12:1.20. The high purity chromatographic material of claim 18 , wherein m is 2 or 3.21. The high purity chromatographic material of claim 18 , wherein the ionizable modifying reagent is 2-(2-(trichlorosilyl)ethyl)pyridine claim 18 , 2-(2-(trimethoxy)ethyl)pyridine claim 18 , 2-(2-(triethoxy)ethyl)pyridine claim 18 , 2-(4-pyridylethyl)triethoxysilane claim 18 , 2-(4-pyridylethyl)trimethoxysilane claim 18 , 2-(4-pyridylethyl)trichlorosilane.22. The high purity chromatographic material of claim 18 , further comprising a chromatographic core material.23. The high purity chromatographic material of claim 22 , wherein the chromatographic core material is a silica material or a hybrid inorganic/organic material.24. The high purity chromatographic material of claim 23 , wherein the chromatographic core material is a superficially porous material.25. The high purity chromatographic material of claim 18 , wherein the hydrophobic surface group is a C4 to C30 bonded phase claim 18 , an aromatic claim 18 , a phenylalkyl claim 18 , a fluoro-aromatic claim 18 , a phenylhexyl claim 18 , a pentafluorophenylalkyl claim 18 , or a chiral bonded phase26. A method for preparing a high purity chromatographic material according to comprising the steps of:reacting a ...

Polymer Shells

The present invention relates to a method for the preparation of polymer shells, preferably composed of cellulose or hemicellulose, comprising the steps of dissolving the polymer component in a first solvent, preferably an organic solvent and precipitating the polymer component by contacting the first solution with a second solvent, which second solvent has a polar character, and in which second solvent the polymer component is essentially insoluble, thereby obtaining polymer shells. Moreover, the invention refers to the polymer shells as such, having permeable and responsive properties, as well as various applications comprising such polymer shells within the fields of drug delivery, separation techniques, and inter alia filling material. 127.-. (canceled)28. A cellulose shell , comprising a shell wall having a thickness from 100 nm to 2 mm and encasing a space comprising a gas , wherein the cellulose shell has an outer diameter between 10 μm and 10 mm.29. The cellulose shell of claim 28 , wherein the outer diameter of the shell is from 10 μm to 5 mm.30. The cellulose shell of claim 28 , wherein the outer diameter of the shell is from 10 μm to 2 mm.31. The cellulose shell of claim 28 , wherein the outer diameter of the shell is from 10 μm to 1 mm.32. The cellulose shell of claim 28 , wherein the shell wall has a thickness from 0.5 μm to 500 μm.33. The cellulose shell of claim 28 , wherein the shell wall has a thickness from 2 μm to 30 μm.34. The cellulose shell of claim 28 , further comprising a molecule comprising xyloglucan attached to the shell.35. A method for preparing the cellulose shell of claim 28 , comprising:(a) dissolving cellulose in a solvent comprising at least one organic solvent to produce a solution of step (a);(b) dissolving a core-forming substance in the solution of step (a) wherein the core-forming substance comprises at least one gas, by feeding said gas into the solution of step (a) or by pressurizing the solution of step (a) with said gas, ...

HYBRID MATERIAL FOR CHROMATOGRAPHIC SEPARATIONS COMPRISING A SUPERFICIALLY POROUS CORE AND A SURROUNDING MATERIAL

The present invention provides novel chromatographic materials, e.g., for chromatographic separations, processes for their preparation and separations devices containing the chromatographic materials. The preparation of the inorganic/organic hybrid materials of the invention wherein a surrounding material is condensed on a superficially porous hybrid core material will allow for families of different hybrid packing materials to be prepared from a single core hybrid material. Differences in hydrophobicity, ion-exchange capacity, chemical stability, surface charge or silanol activity of the surrounding material may be used for unique chromatographic separations of small molecules, carbohydrates, antibodies, whole proteins, peptides, and/or DNA. 1. An inorganic/organic hybrid material comprising an inorganic/organic hybrid surrounding material and a superficially porous core.2. The inorganic/organic hybrid material of claim 1 , wherein the inorganic/organic hybrid surrounding material and the superficially porous core are composed of different materials.3. The inorganic/organic hybrid material of claim 1 , wherein the inorganic/organic hybrid surrounding material and the superficially porous core are composed of the same materials.4. The inorganic/organic hybrid material of claim 1 , wherein the inorganic/organic hybrid surrounding material is composed of a material which enhances one or more of the characteristics selected from the group consisting of chromatographic selectivity claim 1 , particle chemical stability claim 1 , column efficiency claim 1 , and mechanical strength.5. The inorganic/organic hybrid material of claim 1 , wherein the superficially porous core is composed of a material which enhances one or more characteristics selected from the group consisting of chromatographic selectivity claim 1 , particle chemical stability claim 1 , column efficiency claim 1 , and mechanical strength.6. The inorganic/organic hybrid material of claim 4 , wherein the ...

SEPARATION METHOD

The invention relates to a method of isolating an immunoglobulin, comprising the steps of: 1. A method of isolating an immunoglobulin , comprising the steps of:a) providing a separation matrix comprising multimers of immunoglobulin-binding alkali-stabilized Protein A domains covalently coupled to a porous support,b) contacting a liquid sample comprising an immunoglobulin with said separation matrix,c) washing said separation matrix with a washing liquid,d) eluting the immunoglobulin from the separation matrix with an elution liquid, ande) cleaning the separation matrix with a cleaning liquid,{'i': 'Staphylococcus', 'wherein said alkali-stabilized Protein A domains comprise mutants of a parental Fc-binding domain of Protein A (SpA), as defined by, or having at least 80% such as at least 90%, 95% or 98% identity to, SEQ ID NO 51 or SEQ ID NO 52, wherein the amino acid residues at positions 13 and 44 of SEQ ID NO 51 or 52 are asparagines and wherein at least the asparagine residue at position 3 of SEQ ID NO 51 or 52 has been mutated to an amino acid selected from the group consisting of glutamic acid, lysine, tyrosine, threonine, phenylalanine, leucine, isoleucine, tryptophan, methionine, valine, alanine, histidine and arginine.'}2. The method of claim 1 , wherein the glutamine residue at position 1 of SEQ ID NO 51 or 52 has been mutated to an alanine.3. The method of claim 1 , wherein the asparagine or glutamic acid residue at position 35 of SEQ ID NO 51 or 52 has been mutated to an alanine.4. The method of claim 1 , wherein said separation matrix comprises at least 11 mg/ml claim 1 , such as at least 15 mg/ml claim 1 , of said multimers of immunoglobulin-binding alkali-stabilized Protein A domains covalently coupled to a porous support.5. The method of claim 1 , wherein in step b) at least 40 mg immunoglobulin per ml separation matrix is contacted with said separation matrix.6. The method of claim 1 , wherein in step e) said cleaning liquid comprises at least 0.5 M ...

Process for the polymerization of propylene

The invention relates to a process for the preparation of polypropylene having: a molecular weight of 450,000-950,000, a molecular weight distribution of 3-6, and xylene soluble content of 2-6 wt %, by converting propylene into the polypropylene without pre-polymerization in the presence of a polymerization catalyst under a condition where the volume ratio of H 2 to propylene is at most 0.0020, wherein the catalyst comprises a catalyst component and a co-catalyst, wherein the catalyst component is obtained by a process wherein a compound with formula Mg(OAlk) x Cl y wherein x is larger than 0 and smaller than 2, y equals 2-x and each Alk, independently, represents an alkyl group, is contacted with a titanium tetraalkoxide and/or an alcohol in the presence of an inert dispersant to give an intermediate reaction product and wherein the intermediate reaction product is contacted with titanium tetrachloride in the presence of an internal donor.

Methods and Devices for Chromatin Immunoprecipitation Assays

In one aspect, there is provided a method for isolating chromatin from a sample, comprising a step of passing a liquid sample comprising chromatin through a rigid porous matrix on which a ligand is immobilized, wherein the ligand binds to a protein associated with the chromatin. 130-. (canceled)31. A separation column comprising a chamber for holding a liquid sample comprising chromatin , and a rigid porous matrix on which a ligand is immobilized , wherein the ligand is capable of binding to a protein associated with the chromatin , and wherein the rigid porous matrix is positioned within the chamber such that , in use , the liquid sample can be passed through the rigid porous matrix , whereby chromatin present in the liquid sample is separated from the liquid sample as it flows through the rigid porous matrix.32. A separation column according to claim 31 , wherein the rigid porous matrix comprises sintered thermoplastic polymer particles.33. A separation column according to claim 31 , wherein the rigid porous matrix is in the form of a filter disc or frit.34. A separation column according to claim 31 , wherein the rigid porous matrix is positioned above an effluent port of the column claim 31 , such that the liquid sample held in the chamber can be passed through the rigid porous matrix and exit the column claim 31 , thereby isolating chromatin from the liquid sample by binding of chromatin to the rigid porous matrix.35. A separation column according to claim 31 , further comprising a collection vessel for receiving liquid which has passed through the rigid porous matrix and exited the column.36. A separation column according to claim 31 , further comprising a hydrophobic matrix.37. A separation column according to claim 31 , wherein the hydrophobic matrix is positioned between the rigid porous matrix and an effluent port of the column.38. A separation column according to claim 31 , which is a spin column.39. A separation column according to claim 31 , wherein the ...

Method for analyzing triglyceride, method for sorting oil and fat, and method for producing triglyceride

An object of the present invention is to provide a method for analyzing an isomer of triglyceride, a method for sorting oils and fats having different contents of an isomer of triglyceride, and a method for producing triglyceride of which an isomer can be fractionated. The present invention is a method for analyzing triglyceride, including a step of analyzing an isomer of triglyceride by supercritical fluid chromatography, in which multiple types of stationary phases are used in the supercritical liquid chromatography, and at least two stationary phases among the stationary phases have different chiral selectors in each of which one or more chlorines are bound to a polysaccharide derivative.

SOLID SUPPORT INCLUDING A POLYMER AND USE THEREOF

A solid support with a polymer, and a method of using the solid support are provided. 2. The solid support of claim 1 , wherein the fluorocarbon is a substituted or unsubstituted claim 1 , linear or branched compound of fluoro-containing C.3. The solid support of claim 1 , wherein the fluorocarbon is a fluoro-containing Calkyl compound claim 1 , a fluoro-containing Ccarbonyl compound claim 1 , a fluoro-containing Calkoxy compound claim 1 , or a combination thereof.4. The solid support of claim 1 , wherein the biomolecule is selected from the group consisting of a protein claim 1 , a nucleic acid claim 1 , and a sugar.5. The solid support of claim 1 , wherein the material specifically binding to a biomolecule is selected from the group consisting of a protein claim 1 , a nucleic acid claim 1 , a sugar claim 1 , and a cell.6. The solid support of claim 1 , wherein the material specifically binding to a biomolecule is an antibody claim 1 , an antigen against an antibody claim 1 , a receptor against a ligand claim 1 , a ligand against a receptor claim 1 , a substrate or inhibitor of an enzyme claim 1 , or an enzyme against a substrate or inhibitor.7. The solid support of claim 1 , wherein the material specifically binding to a biomolecule is Protein G claim 1 , Protein A claim 1 , lectin claim 1 , an antibody claim 1 , avidin claim 1 , streptavidin claim 1 , a receptor protein claim 1 , or a combination thereof.8. The solid support of claim 1 , wherein the solid support comprises a bead claim 1 , a plate claim 1 , or a well on which the at least one polymer is immobilized.9. The solid support of claim 1 , wherein about 10% to about 90% of the number of repeating units of the polymer comprises at least one material that specifically binds to a biomolecule.10. The solid support of claim 1 , wherein about 10% to about 90% of the number of repeating units of the polymer comprises a fluorocarbon.11. The solid support of claim 1 , wherein the polymer comprises about 1 to ...

PACKING MATERIAL FOR LIQUID CHROMATOGRAPHY

Provided is a packing material for liquid chromatography, including a gel obtained by polymerizing monomers including 40% by mass or more of a crosslinkable monomer having a (meth)acryloyloxy group. 1. A packing material for liquid chromatography , which is made of a gel obtained by polymerizing monomers including 40% by mass or more of a crosslinkable monomer having a (meth)acryloyloxy group.2. The packing material for liquid chromatography according to claim 1 ,wherein the crosslinkable monomer having a (meth)acryloyloxy group is at least one selected from the group consisting of an ester of a (poly)alkylene glycol and a (meth)acrylic acid, an ester of a glycerin and a (meth)acrylic acid, and an ester of a pentaerythritol and a (meth)acrylic acid.6. The packing material for liquid chromatography according to claim 1 ,wherein the monomers further include a non-crosslinkable monomer which is a compound having one ethylenic double bond in the molecule thereof, in addition to the crosslinkable monomer having a (meth)acryloyloxy group.8. A size exclusion liquid chromatography comprising:introducing a sample capable of being dissolved in an aqueous solvent into a column filled with a packing material for liquid chromatography to perform detection using a light scattering detector,{'claim-ref': {'@idref': 'CLM-00001', 'claim 1'}, 'wherein the packing material includes the gel according to .'}9. The packing material for liquid chromatography according to claim 1 ,wherein the gel is a porous polymer gel and the volume-average particle diameter of the packing material is 1 to 500 microns.10. The packing material for liquid chromatography according to claim 1 ,wherein the gel is a polymer gel including 40% by mass or more of a monomer unit derived from the crosslinkable monomer having a (meth)acryloyloxy group.11. The packing material for liquid chromatography according to claim 1 ,wherein the crosslinkable monomer is a compound having two or more ethylenic double bonds in ...

SPECIFIC SORBENT FOR BINDING PROTEINS AND PEPTIDES, AND SEPARATION METHOD USING THE SAME

Sorbent comprising a solid support material, the surface of which comprises first residues comprising a binuclear heteroaromatic structure comprising besides carbon atoms at least one of the heteroatoms N, O, S, and second residues comprising a mononuclear heteroaromatic structure comprising besides carbon atoms at least one of the heteroatoms N, O, S. 2. Sorbent of claim 1 , wherein the binuclear heteroaromatic structure is a benzopyrrole (indole) structure claim 1 , including all possible aza-benzopyrrole claim 1 , oxa-benzopyrrole claim 1 , and thia-benzopyrrole structures claim 1 , or a benzopyridine (quinoline or isoquinoline) structure claim 1 , including all possible aza-benzopyridine claim 1 , oxa-benzopyridine claim 1 , and thia-benzopyridine structures.3. Sorbent of claim 1 , wherein the mononuclear heteroaromatic structure is a pyrrole structure claim 1 , including all possible aza-pyrrole claim 1 , oxa-pyrrole claim 1 , and thia-pyrrole structures such as 3-azapyrrole (imidazole).4. Sorbent of claim 1 , wherein the first and/or second residue comprise(s) a covalent claim 1 , conformational̂flexible linker of a length of from 1 to 20 atoms.5. Sorbent of claim 1 , wherein the surface of the solid support material additionally comprises a third residue and optionally a fourth residue.6. Sorbent of claim 5 , wherein the third residue comprises an amine or amide structure or a primary amine structure.7. Sorbent of claim 6 , wherein the first claim 6 , second claim 6 , and third residue are present in a molar ratio of about 1:1:2.8. Sorbent of claim 1 , wherein the surface of the solid support material is covered with a film of a polymer comprising a first and a second functional group claim 1 , which may be the same or which may be different from each other claim 1 , which in turn carry said first and second claim 1 , and optionally a third and a fourth residue.9. Sorbent of claim 8 , wherein the polymer comprises or consists of individual chains which are ...

TWO-PHASE ELECTROEXTRACTION FROM MOVING PHASES

The present invention relates to a process for the electro extraction of molecules from a moving fluid donor phase into an acceptor phase, comprising the steps of: providing an electrically conductive donor phase moving at a first flow velocity and in electrically conductive contact with a first electrode, providing an electrically conductive acceptor phase in direct contact and immiscible with the donor phase, in electrically conductive contact with a second electrode; and providing a supporting or confining phase guide pattern to keep a defined interface between donor phase and acceptor phase, and (d) applying an electrical field between the first and the second electrode. 1. A process for the electroextraction of compounds from a moving fluid donor phase into an acceptor phase , comprising the steps of:a) providing an electrically conductive donor phase moving at a first flow velocity and in electrically conductive contact with a first electrode,b) providing an electrically conductive acceptor phase in direct contact and immiscible with the donor phase, in electrically conductive contact with a second electrode; andc) providing a supporting or confining phase guide pattern to keep a defined interface between donor phase and acceptor phase, and(d) applying an electrical field between the first and the second electrode.2. A process according to claim 1 , wherein the acceptor phase is moving co-directionally with the donor phase at a second flow velocity lower than the first flow velocity claim 1 , preferably stagnant.3. A process according to any one of the previous claims claim 1 , wherein the donor and/or acceptor phase remain immiscible during the time scale and under the conditions of the process.4. A process according to any one of the previous claims claim 1 , wherein the electrical field is applied sufficiently high and in a sufficiently long period of time to allow at least part of the analyte compounds to migrate from the donor phase to the acceptor phase ...

ELECTROKINETIC DEVICE FOR CAPTURING ASSAYABLE AGENTS IN A DIELECTRIC FLUID UTILIZING REMOVABLE ELECTRODES

Electrokinetic devices and methods are described with the purpose of collecting assayable agents from a dielectric fluid medium. Electrokinetic flow may be induced by the use of plasma generation at high voltage electrodes and consequent transport of charged particles in an electric voltage gradient. In one embodiment, an ionic propulsion device for providing a sample for a bio-specific assay of aerosol particles comprises a housing receiving a sample of aerosol particles and enclosing a high voltage electrode to generate a plasma of electrically charged particles. A carrier assembly is removably receivable in the housing, the carrier assembly comprising a non-conductive carrier and an electrode removably secured to the carrier. Incident to the carrier assembly being received in the housing, the electrode is subject to a voltage so that flow of charged aerosol particles generates a net air flow through the housing and said charged aerosol particles are deposited on the electrode, and said electrode can be removed from said carrier and placed in an extraction vessel for a bio-specific assay. 1. An ionic propulsion device for providing a sample for a bio-specific assay of aerosol particles , comprising:a housing receiving a sample of aerosol particles and enclosing a high voltage electrode to generate a plasma of electrically charged particles; anda carrier assembly removably receivable in the housing, the carrier assembly comprising a non-conductive carrier and a capture electrode removably secured to the carrier,wherein, incident to the carrier assembly being received in the housing, the high voltage electrode is subject to a voltage so that flow of charged aerosol particles generates a net air flow through the housing and said charged aerosol particles are deposited on the capture electrode, and said capture electrode can be removed from said carrier and placed in an extraction vessel for a bio-specific assay.2. The ionic propulsion device of wherein said carrier ...

POROUS INORGANIC/ORGANIC HOMOGENOUS COPOLYMERIC HYBRID MATERIALS FOR CHROMATOGRAPHIC SEPARATIONS AND PROCESS FOR THE PREPARATION THEREOF

The present invention relates to porous inorganic/organic homogenous copolymeric hybrid material materials, including particulates and monoliths, methods for their manufacture, and uses thereof, e.g., as chromatographic separations materials. 1140-. (canceled)143. The material according to claim 141 , wherein 0.003≦y/z≦50 and 0.02≦x/(y+z)≦21.144. The material according to claim 141 , wherein said material has a specific surface area of about 50-800 m/g.145. The material according to claim 141 , wherein said material is hydrolytically stable at a pH of about 1 to about 13.146. The material according to claim 142 , wherein 0.003≦y/z≦50 and 0.02≦x/(y+z)≦21.147. The material according to claim 142 , wherein said material has a specific surface area of about 50-800 m/g.148. The material according to claim 142 , wherein said material is hydrolytically stable at a pH of about 1 to about 13.149. A separations device comprising a material according to .150. The separations device according to claim 149 , wherein said device is selected from the group consisting of chromatographic columns claim 149 , thin layer plates claim 149 , filtration membranes claim 149 , sample cleanup devices claim 149 , and microtiter plates.151. A separations device comprising a material according to .152. The separations device according to claim 151 , wherein said device is selected from the group consisting of chromatographic columns claim 151 , thin layer plates claim 151 , filtration membranes claim 151 , sample cleanup devices claim 151 , and microtiter plates. This application claims benefit of and is a continuation of International Application No. PCT/US03/34776, filed Oct. 30, 2003 and designating the United States, which claims benefit of and priority to U.S. Provisional Application No. 60/422,580, filed Oct. 30, 2002. The entire contents of these applications are incorporated herein by reference.Packing materials for liquid chromatography (LC) are generally classified into two types: ...

Particle of Modified Silica

The present invention relates to a particle of modified silica which comprises a particle of silica which is covalently linked to at least one halohydrin moiety. Furthermore, the present invention relates to a method of preparing the particle of modified silica, a particle of modified silica obtained by such method, use of the particle of modified silica as a stationary phase for chromatography, as well as a separation column for chromatography comprising the particle of modified silica. 1. A particle of modified silica which comprises a particle of silica which is covalently linked to at least one halohydrin moiety.3. The particle of modified silica according to claim 1 , wherein the silica is in the form of a porous particle or a monolithic material.4. The particle of modified silica according to claim 2 , wherein A is chlorine.5. The particle of modified silica according to claim 2 , wherein Rcomprises an aryl moiety.6. The particle of modified silica according to claim 2 , wherein Rcomprises a steroid moiety.7. The particle of modified silica according to claim 2 , wherein Rcomprises R—O—CH— claim 2 , and Ris selected from hydrocarbons having from 1 carbon atom up to 30 carbon atoms.9. The particle of modified silica according to claim 1 , wherein the surface density of the halohydrin moiety to particle of silica is from 0.1 to 4 μmoles/msilica surface.10. A method of preparing a particle of modified silica comprising the steps of reacting a particle of silica and at least one halohydrin compound to form a covalent bond between said particle of silica and said halohydrin compound.12. The method according to claim 10 , wherein the particle of silica is in the form of a porous particle or a monolithic material.13. A particle of modified silica obtainable by the method as claimed in .14. A stationary phase for chromatography comprising the particle of modified silica of .15. A separation column for chromatography comprising the particle of modified silica according ...

Chromatographic material and method for preparation thereof

A chromatographic material comprising a zwitterionic ligand covalently bound to a substrate, the ligand preferably has a formula II: wherein R 1 , R 2 , R 3 are independently selected from an oxygen atom that is configured to connect to a substrate atom in the substrate, an oxygen atom that is configured to connect to a silicon atom of an adjacent ligand, a hydroxyl group, a halogen atom, an alkoxy group, a dialkylamino group, an acyl group, an alkyl group, or an aryl group; L 1 , L 2 and L 3 are independently hydrophobic moieties; each containing 2 to 30 carbon atoms, wherein there are at least 10 carbon atoms in the combined chain lengths of L 1 , L 2 and L 3 ; X is an O atom, S atom, amide group or sulfonamide group; n is 0 or 1; R 4 , R 5 are independently selected from a hydrogen atom or a hydrocarbon moiety containing 1 to 20 carbon atoms; and R f is a negatively charged moiety comprising a sulfonic, carboxylic, or phosphonic functional group.

SEPARATION OF VANCOMYCIN AND ITS DEGRADATION PRODUCTS

Disclosed is a chromatographic method for separating a mixture of compounds having ionizable groups using a mobile phase comprising (a) a first mobile phase component comprising an aqueous buffer system and an organic solvent mixture miscible with water, and (b) a second mobile phase component comprising an aqueous buffer system and an organic solvent mixture miscible with water, wherein the buffer system and the solvent mixture in the first mobile phase component are different from the buffer system and the solvent mixture in the second mobile phase component and the ratio of the first mobile phase component to the second mobile phase component is varied during the separation. The method can be used for the separation of vancomycin and its degradation products. 1. A method for separating a mixture of compounds each having at least one ionizable group , the method comprising: treating the mixture with reversed phase high pressure liquid chromatography on a stationary phase comprising a reversed phase chromatography column in the presence of a mobile phase comprising (a) a first mobile phase component comprising an aqueous buffer system comprising a weak acid and optionally a conjugate base , and at least one organic solvent miscible with water , and (b) a second mobile phase component comprising an aqueous buffer system comprising a conjugate base and optionally a weak acid , and a different concentration of at least one organic solvent miscible with water , wherein the ratio of weak acid to conjugate base in the first mobile phase component is different from the ratio of weak acid to conjugate base in the second mobile phase component and the ratio of the first mobile phase component to the second mobile phase component is varied during the separation.2. The method of wherein the at least one miscible organic solvent is selected from the group consisting of alcohols claim 1 , acetonitrile claim 1 , dioxane claim 1 , tetrahydrofuran and mixtures thereof.3. The ...

CURTAIN FLOW DESIGN FOR OPTICAL CHAMBERS

Various embodiments include systems and apparatuses for reducing contamination levels within optical chambers of particle-detection instruments. In one embodiment, an apparatus to reduce contamination within an optical chamber of a particle-detection instrument is described. The apparatus includes a plenum chamber to at least partially surround an aerosol-focusing nozzle of the particle-detection instrument and accept a filtered gas flow. A curtain-flow concentrating nozzle is coupled to the plenum chamber to produce a curtain flow into the optical chamber to substantially surround an aerosol flow. Other methods and systems are disclosed. 1. An apparatus to reduce contamination within an optical chamber of a particle-detection instrument , the apparatus comprising:a plenum chamber to at least partially surround an aerosol-focusing nozzle of the particle-detection instrument, the plenum chamber to accept a filtered gas flow; anda curtain-flow concentrating nozzle coupled to an output of the plenum chamber to produce a curtain flow into the optical chamber to substantially surround an aerosol flow.2. The apparatus of claim 1 , further comprising an open area coupled to an output of the curtain-flow concentrating nozzle and at least partially surrounding the aerosol-focusing nozzle.3. The apparatus of claim 2 , wherein a combination of the plenum chamber claim 2 , the curtain-flow concentrating nozzle claim 2 , and the open area are arranged to provide a clean sheath of airflow around the aerosol flow.4. The apparatus of claim 2 , wherein a combination of the plenum chamber and the curtain-flow concentrating nozzle is to substantially equalize flow pressure such that the curtain flow is distributed substantially evenly around an upper portion of the aerosol-focusing nozzle before passing from an outlet of the curtain-flow concentrating nozzle into the open area surrounding the upper portion claim 2 , through the outlet.5. The apparatus of claim 1 , wherein the plenum ...

Hand-held adipose processor and cell concentrator

Devices and methods are provided for processing adipose tissue with a hand-held device. This device may include a processing chamber, a cannula, a vacuum source, a digestion area, and a product cell concentration chamber.

PREPARATION METHOD OF CHROMATOGRAPHY MEDIUM

A method for preparing a chromatography medium having the properties of high virus adsorption and high fluidity, and a method for producing a virus vaccine using the chromatography medium are provided. The chromatography medium is obtained by forming a sulfated polysaccharide bound with porous particles having an exclusion limit molecular weight of 6000 Da or less when pure water is used as mobile phase and standard polyethylene glycol is used and an average particle size in the range of 30-200 μm. 1. A method for preparing a chromatography medium , comprising:step 1: cross-linking porous particles with a polysaccharide by using a cross-linking agent having two or more functional groups, to obtain polysaccharide-binding porous particles, wherein the porous particles have an exclusion limit molecular weight of 6000 Da or less when pure water is used as mobile phase and standard polyethylene glycol is used; and {'br': None, 'sub': 2', '2', '2, 'X—O—CH—C(OH)H—CH—NH—CH—Z\u2003\u2003(1)'}, 'step 2: sulfating the polysaccharide-binding porous particles obtained in step 1 with a sulfating agent to obtain the chromatography medium represented by General Formula (1) belowwherein in Formula (1), X is the porous particle have an average particle size in the range of 30-200 μm, O bonded to X is an oxygen originating from hydroxyl of the porous particle, Z is the sulfated polysaccharide, and C bonded to Z is a carbon originating from the reductive end of the sulfated polysaccharide, wherein the sulfated polysaccharide is a ligand, and a limiting viscosity of the sulfated polysaccharide is in the range of 0.40-0.90 dL/g.2. The method for preparing the chromatography medium according to claim 1 , wherein the porous particles have an average particle size in the range of 40-120 μm.3. The method for preparing the chromatography medium according to claim 1 , wherein the porous particles are cellulose particles.4. The method for preparing the chromatography medium according to claim 1 ...

POROUS FIBER, ADSORBENT MATERIAL, AND PURIFICATION COLUMN

A solid fiber has a modified cross-section which satisfies the following (a) to (b): (a) a modification degree Do/Di, in a cross section of the solid fiber, is 1.20 to 8.50 when the inscribed circle diameter is denoted by Di and the circumscribed circle diameter is denoted by Do; and (b) a porous specific surface area of the fiber is not less than 30 m/g. An adsorbent material comprises not less than 28 vol % of the porous fiber as a fiber bundle. A purification column is formed by arranging the adsorbent material in the straight form in an axis direction of a plastic casing and by attaching an inlet port and an outlet port of a fluid that is to be treated to both ends of the plastic casing. The porous fiber can efficiently adsorb a removal target substance in the fluid that is to be treated, and a purification column incorporates the porous fiber. 1. A porous fiber comprising a modified solid cross-section , wherein the porous fiber satisfies the following (a) to (b):(a) a modification degree Do/Di, in a cross section of the solid fiber, is 1.20 to 8.50 when the inscribed circle diameter is denoted by Di and the circumscribed circle diameter is denoted by Do; and{'sup': '2', '(b) a porous specific surface area of the fiber is not less than 3 m/g.'}2. The porous fiber according to claim 1 , wherein an average radius of pore is not less than 0.8 nm and not more than 90 nm.3. The porous fiber according to claim 1 , wherein the porous specific surface area is not less than 30 m/g.4. The porous fiber according to claim 1 , wherein an inscribed circle occupancy of the following equation is not less than 0.10:{'br': None, 'Inscribed circle occupancy=Area of the inscribed circle of the cross section of the fiber/Area of the cross section of the fiber.'}5. The porous fiber according to claim 1 , wherein a diameter of pore in the porous fiber is not more than 25 μm and a ratio of an average diameter of pore in the area adjacent to the outer surface of the fiber vs an average ...

COMPOSITIONS, KITS AND METHODS USEFUL FOR ANALYZING ANTIBODY-CONTAINING SAMPLES

In some aspects, the present disclosure pertains to sample treatment methods that comprise: contacting an acidic elution solution that is free of primary amine, secondary amine and thiol groups with a sorbent having bound target antibody and separating the elution solution from the sorbent, thereby releasing bound target antibody from the sorbent and forming a first collection fraction that comprises the elution solution and released target antibody; contacting the sorbent with a neutralization buffer solution that is free of primary amine, secondary amine and thiol groups and separating the neutralization buffer solution from the sorbent, thereby forming a second collection fraction that comprises the neutralization buffer solution; and forming a neutralized solution that comprises the first collection fraction and the second collection fraction. In other aspects, the present disclosure pertains to kits for performing such sample treatment methods. 1. A sample treatment method comprising:(a) contacting a sample fluid that contains a target antibody with a sorbent that has affinity for the target antibody and separating the sample fluid from the sorbent, thereby forming a sorbent having bound target antibody;(b) contacting a washing solution that is free of primary amine, secondary amine and thiol groups with the sorbent having bound target antibody and separating the washing solution from the sorbent having bound target antibody, thereby removing unbound molecules from the sorbent having bound target antibody while leaving target antibody bound to the sorbent;(c) contacting an acidic elution solution that is free of primary amine, secondary amine and thiol groups with the sorbent and separating the elution solution from the sorbent, thereby releasing bound target antibody from the sorbent and forming a first collection fraction that comprises the elution solution and released target antibody;(d) contacting the sorbent with a neutralization buffer solution that is ...

Separating agent for optical isomer

Provided is a novel separating agent for optical isomers based on a helical polymer having optically active sites. The separating agent for optical isomers has a helical polymer having a structure represented by Formula (I), and a carrier that supports the helical polymer, wherein the helical polymer is supported by the carrier. (In Formula (I), X represents a divalent aromatic group, a single bond or a methylene group; R represents hydrogen or a C1-C5 alkoxy; and n represents an integer equal to or higher than 5. When X is a divalent aromatic group, Y represents —CONH—, —COO—, —NHCONH—, —NHCSNH—, —SO 2 NH— or —NHCOO—, and when X is a single bond or a methylene group, Y represents —COO—, —NHCONH—, —NHCSNH— or —NHCOO—.)

Porous Material And Devices For Performing Separations, Filtrations, And Catalysis And EK Pumps, And Mthods Of Making And Using The Same

Embodiments of the present invention are directed to a porous monolith polymeric composition having utility in catalysis, chromatography, filtration, and electro-kinetic pumps, devices incorporating such composition and methods or making and using such monoliths. The monoliths are characterized by a substantially homogeneous skeletal core with little shrinkage, few voids and few channels. 1. A composition of matter comprising a monolith having a skeletal core and pores , said skeletal core having a substantially homogeneous polymeric composition of two or more organic silane monomers , said pores defining an interstitial volume in the skeletal core and have a pore size distribution in which there are at least macropores and less than 5% of the interstitial volume is mesopores , said macropores allowing fluid movement through the monolith.2. The composition of matter of in which said interstitial volume has fewer than 2% mesopores.3. The composition of matter of wherein said interstitial volume has fewer than 1% mesopores.4. The composition of matter of wherein said skeletal core has polymers which deviate from the substantially homogeneous polymeric composition to form nodules claim 1 , said nodules have a nodule cross-sectional diameter and said skeletal core having a substantially homogeneous polymeric composition without a nodule has a core cross-sectional diameter wherein the ratio of the nodule cross sectional diameter to core cross sectional diameter is less than 80 to 1.5. The composition of matter of wherein said skeletal core has polymers which deviate from the substantially homogeneous polymeric composition to form nodules claim 1 , said nodules have a nodule cross-sectional diameter and said skeletal core claim 1 , having a substantially homogeneous polymeric composition without a nodule claim 1 , has a core cross-sectional diameter wherein the ratio of the nodule cross sectional diameter to core cross sectional diameter is less than 50 to 1.6. The ...

SYSTEM AND METHOD FOR TRACE SAMPLE PRECOLLECTION AND PRECONCENTRATION

A device and procedure are described that can be used for improved sampling of traces such as for explosive trace screening, by precollection and preconcentration of trace samples of vapor and particulate matter from air. The device is unique in its ability to collect both solid and vapor traces. 1. A method for screening packed cargo comprising steps of:forcing a working fluid into said packed cargo by means of at least one gas input hose; andexhausting said working fluid from said packed cargo by means of at least one gas exhaust hose;whereby packed cargo is penetrated by said working fluid and exhausted.2. The method of further said packed cargo for encouraging dispersing of trace material from said cargo into said fluid stream.3. The method of further comprising step of trace analysis by using at least one material having chemical affinity for substances of interest.4. The method of further comprising step of controlling the pressure in said input hose and said output hose.5. The method of further comprising step of creating predefined profile of pressure vs. time.6. The method of further comprising step of controlling temperature of said fluid encouraging dispersing of trace material from said cargo into said fluid stream.7. The method of wherein said fluid is selected from the group consisting of: air claim 1 , nitrogen claim 1 , argon claim 1 , helium claim 1 , hydrogen claim 1 , oxygen claim 1 , carbon dioxide claim 1 , trace-reactive molecules claim 1 , and combinations thereof.8. The method of further comprising step of introducing a relatively high electric potential into said fluid stream encouraging ionization of elements of said fluid stream.9. The method of further comprising step of puncturing gastight covering of said cargo.10. The method of wherein extant apertures of said packed cargo are used for purposes of input and exhaust of said fluid.11. The method of further comprising steps of:constricting said fluid stream in a bottleneck tending to ...

POROUS MATERIALS WITH CONTROLLED POROSITY; PROCESS FOR THE PREPARATION THEREOF; AND USE THEREOF FOR CHROMATOGRAPHIC SEPARATIONS

The present invention provides novel chromatographic materials. e.g., for chromatographic separations, processes for their preparation and separations devices containing the chromatographic materials. The chromatographic materials of the invention have controlled porosity and comprise a chromatographic core material and one or more layers of chromatographic surface material which each independently provide an average pore diameter, an average pore volume, or a specific surface area such that the combined layers form a chromatographic material having a predetermined or desired pattern of porosity from the core material to the outermost surface. The materials are useful for HPLC separations, normal-phase selarations, reversed-phase separations, chiral separations, HILIC separations, SFC separations, affinity separations, perfusive separations, partially perfusive separations, and SEC separations. 1. A chromatographic material having controlled porosity.2. The chromatographic material of claim 1 , wherein the material is in the form of a particle.3. The chromatographic material of claim 1 , wherein the material is in the form of a monolith.4. The chromatographic material of claim 1 , wherein the material is in the form of a superficially porous material.5. (canceled)6. (canceled)7. The chromatographic material of claim 1 , comprising a chromatographic core material having a primary surface and one or more layers of a chromatographic surface material.8. (canceled)9. The chromatographic material of claim 7 , wherein the chromatographic core material is an inorganic material claim 7 , an organic material claim 7 , or an inorganic/organic hybrid material.10. (canceled)1222-. (canceled)23. The chromatographic material of claim 7 , wherein each layer of the one or more layers of a chromatographic surface material independently has an average pore diameter of 20 to 1500 Angstroms.24. The chromatographic n material of claim 23 , wherein the average pore diameter of the one or ...

PURIFICATION DEVICE, AND PURIFICATION METHOD

Provided is a purification device for purifying a useful substance (i.e., target substance) without using an acidic eluent. The purification device purifying the target substance comprises a column and a filler packed in the column. The filler contains a carrier, a temperature responsive polyamino acid bonded to the carrier, and a binding substance bonded to the temperature responsive polyamino acid and specifically bonding to the target substance. 1. A purification device for purifying a target substance , comprising a column and a filler packed in the column , whereinthe filler comprises a carrier, a temperature responsive polyamino acid bonded to the carrier, and a binding substance bonded to the temperature responsive polyamino acid and specifically bonding to the target substance.2. The purification device as described in claim 1 , whereinthe temperature responsive polyamino acid changes a structure between a conformation in which the target substance is bounded to the binding substance and a conformation in which the target substance thus bonded to the binding substance is separated therefrom, andthe structural change occurs in the range from 0° C. to 60° C.3. The purification device as described in claim 1 , whereinthe temperature responsive polyamino acid is at least a member selected from a group of polylysine, polyglutamic acid and polyasparagine.4. The purification device as described in claim 1 , wherein the binding substance is Protein A and the target substance is an antibody.5. The purification device as described in claim 1 , further comprising a temperature controller which is configured to change a temperature of the temperature responsive polyamino acid in the range from 0° C. to 60° C.6. A purification method for purifying a target substance claim 1 , the method comprising:a binding step of passing a solution containing a target substance and impurities through a column packed with a filler comprising a carrier, a temperature responsive polyamino ...

Chromatography Media and Use Thereof

The present invention relates to a novel non-cross linked chromatography media provided with glutathione ligands which may or may not be provided with magnetic particles. The chromatography media is used for production of an affinity media provided with gluthatione ligands for adsorption of GST-tagged proteins. 1. Non-cross linked chromatography media provided with glutathione ligands.2. Chromatography media according to claim 1 , which is provided with magnetic particles.3. Chromatography media according to claim 1 , which is based on agarose.4. Use of non-cross linked chromatography media for production of an affinity media provided with gluthatione ligands for adsorption of GST tagged proteins.5. Use according to claim 4 , wherein the chromatography media is provided with magnetic particles.6. Use according to claim 4 , wherein the chromatography media is based on agarose.7. Use according to claim 6 , wherein the chromatography media is Sepharose™.8. Use according to claim 4 , wherein the GST tagged proteins are fed directly after cell culture to the chromatography media.9. Use according to claim 8 , wherein the GST tagged proteins are concentrated after cell culture before loading onto the chromatography media. This is a national stage application under 35 U.S.C. §371(c) of prior-filed, co-pending, PCT application serial number PCT/SE2013/050662, filed on Jun. 11, 2013, which claims priority to Swedish patent application serial number 1250625-9, filed on Jun. 14, 2012, the entire contents of which are incorporated by reference herein.The present invention relates to a novel chromatography media and use thereof. More closely it relates to a method for coupling of GSH to a non cross-linked chromatographic media provided with magnetic particles.The use of affinity tags that are genetically coded and linked to a target protein to facilitate its purification is well established in the research community. Examples of commonly used such tags are glutathione-S- ...

Apparatus and method for rapid chemical analysis using differential desorption

The present invention is directed to a method and device to generate a chemical signature for a mixture of analytes. The present invention involves using a SPME surface to one or both absorb and adsorb the mixture of analytes. In an embodiment of the invention, the surface is then exposed to different temperature ionizing species chosen with appropriate spatial resolution to desorb a chemical signature for the mixture of analytes.

Sampling unit for concentrates preparation device

The invention relates to a sampling unit for the concentrates preparation device. The unit comprises a base body, a movable rod, a limiting pin, a sampling chamber, a sampling bore, an axle sleeve and an isobaric chamber. The sampling chamber passes through the upper and lower surfaces of the base body, the isobaric chamber is a cylindrical blind bore, the isobaric chamber passes through the front surface of the base body and passes through the front and back surfaces of the sampling chamber. The axle sleeve has an interference fitting with the isobaric chamber, the movable rod hermetically slidingly mates with the axle sleeve, the head and tail ends of the gas permeable passage respectively communicate with the isobaric chamber and the outside. Concentrates can be taken out through the sampling bore of the sampling unit, the movable rod can take samples freely with the help of gas permeable passage for keeping the pressure balance of ends of the movable rod.

Irradiated Agarose, Compositions Thereof, and Related Methods

Irradiated agarose gels and compositions containing irradiated agarose gels are described, along with methods of production and use. Methods of forming an irradiated agarose composition include irradiating an agarose in dry form to produce an irradiated agarose, dissolving the irradiated agarose in a solvent to form a solution containing irradiated agarose, and gelling the solution containing irradiated agarose to form a gel containing irradiated agarose. The resulting gel containing irradiated agarose may have a reduced gel strength, making it more suitable for use as an injectable, even at high concentrations. 1. A method of forming an irradiated agarose composition , the method comprising:irradiating an agarose in dry form to produce an irradiated agarose;dissolving the irradiated agarose in a solvent to form a solution containing irradiated agarose; andgelling the solution containing irradiated agarose to form a gel containing irradiated agarose.2. The method of claim 1 , wherein the agarose is irradiated in dry form with at least 5 kilograys (kGy) of radiation.3. The method of claim 1 , wherein the agarose is irradiated in dry form with at least 40 kilograys (kGy) of radiation.4. The method of claim 1 , wherein the solvent is water and the solution contains between 3% and 10% irradiated agarose by weight.5. The method of claim 1 , wherein gelling the solution is accomplished by chemical cross-linking or cooling.6. The method of claim 1 , wherein the gel containing irradiated agarose has an agarose concentration of at least 3%.7. The method of claim 1 , wherein the gel containing irradiated agarose has an agarose concentration of at least 5%.8. The method of claim 6 , wherein the gel containing irradiated agarose has a gel strength of less than 2 claim 6 ,000 gm/cm.9. The method of claim 7 , wherein the gel containing irradiated agarose has a gel strength of less than 2 claim 7 ,000 gm/cm.10. The method of claim 1 , wherein the gel containing irradiated agarose ...