Isolation, expansion and characterization of precursor/stem cells from dental tissues
Номер патента: MY166810A
Опубликовано: 23-07-2018
Автор(ы): GOVINDASAMY Vijayendran, KATHIVALOO Premasangery
Принадлежит: Hygieia Innovation Sdn Bhd
Опубликовано: 23-07-2018
Автор(ы): GOVINDASAMY Vijayendran, KATHIVALOO Premasangery
Принадлежит: Hygieia Innovation Sdn Bhd
Реферат: The present invention presents a method for isolating and proliferating at least one type of precursor cell from dental origin from a single donor which includes the following steps of (a) isolating the precursor cells from dental origin of a single donor in a sample collection media and preparing a primary stock culture; (b) proliferating the primary stock culture sequentially to obtain a first, second and third sub-cultured stocks with cell counts ranging between 5 x 106 cells and 10 x 106 cells, 20 x 106 cells and 400 x 106 cells , 150 x 106 and 300 x 106 cells respectively and (c) harvesting and cryo-preserving the precursor cells from the third subculture to obtain a precursor cell population capable of being used for cell transplantation. The dental origin of the precursor cells is at least one selected from the group consisting of pulp, apical papilla and periodontal ligament. The precursor cell is at least one selected from the group consisting of mesenchymal stem cells, ecto-mesenchymal cells, neural stem cells, dental progenitor cells and CD117+ cells. Most illustrative figure is
Isolation, expansion and characterization of precursor/stem cells from dental tissues
Номер патента: US09839654B2. Автор: Vijayendran GOVINDASAMY,Premasangery KATHIVALOO. Владелец: Hygieia Innovation Sdn Bhd. Дата публикации: 2017-12-12.