Heterocyclic fret dye cassettes for labeling biological molecules and their use in dna sequencing
Номер патента: EP1573068A1
Опубликовано: 14-09-2005
Автор(ы): Satyam Nampalli, Shiv Kumar, Sudhakar Rao, Weihong Zhang
Принадлежит: Amersham Biosciences Corp
Опубликовано: 14-09-2005
Автор(ы): Satyam Nampalli, Shiv Kumar, Sudhakar Rao, Weihong Zhang
Принадлежит: Amersham Biosciences Corp
Реферат: Exploitation of suitably functionalized heterocyclic molecules, in the design and synthesis of Fluorescence Resonance Energy Transfer (FRET) cassettes and their corresponding dideoxynucleotide terminators culminated into efficient reagents for DNA sequencing. Additionally, these FRET cassettes/terminators, of the present invention, derived from different classes of heterocyclic systems have high potential to be used for general labelling of biological molecules to generate highly sensitized signals. Their preparation, energy transfer efficiency, and use as labels, specifically, in DNA sequencing reactions is disclosed.
Heterocyclic fret dye cassettes for labeling biological molecules and their use in dna sequencing
Номер патента: EP1573068A4. Автор: Shiv Kumar,Weihong Zhang,Satyam Nampalli,Sudhakar Rao. Владелец: Amersham Biosciences Corp. Дата публикации: 2006-01-04.