A method of separating bispecific antibodies
Номер патента: EP4363432A1
Опубликовано: 08-05-2024
Автор(ы): Bengt Westerlund, Mats Ander
Принадлежит: Cytiva Bioprocess R&D AB
Опубликовано: 08-05-2024
Автор(ы): Bengt Westerlund, Mats Ander
Принадлежит: Cytiva Bioprocess R&D AB
Реферат: The present invention relates to a method of separating bispecific antibodies or bispecific antibody fragments. The method comprises the steps of a) providing a feed comprising bispecific antibodies or bispecific antibody fragments; b) contacting the feed with a separation matrix having affinity ligands coupled to a support; c) optionally washing the separation resin with a washing liquid; d) applying an elution buffer to the separation resin, to elute the antibodies or antibody fragments bound to the affinity ligand; wherein in step d) a pH gradient is applied over the elution buffer, said pH gradient being from about 6 to about 2.
A method of separating bispecific antibodies
Номер патента: US20240228530A1. Автор: Mats Ander,Bengt Westerlund. Владелец: Cytiva Bioprocess R&D AB. Дата публикации: 2024-07-11.