알파-망고스틴, 베타-망고스틴, 감마-망고스틴 또는 가르탄닌 화합물을 유효성분으로 함유하는 피부 주름 개선 또는 피부 보습용 조성물

The present invention refers to muscular alpha -, beta - muscular, gamma - (gartanin) compound of at least one compound selected from muscular and whether the tannin containing improvement of skin wrinkle or skin moisturizing cosmetic composition are disclosed.

Said muscular alpha -, beta - muscular, gamma - (gartanin) compounds are preferably separated from extract tannin muscular and whether the muscular ethanol but has the limited, said muscular alpha -, beta - muscular, such as muscular and gamma - whether tannin (gartanin) compound that is formula can exhibit.

Said muscular alpha -, beta - muscular, gamma - whether MMP-a 9 protein expression dose tannin (gartanin) compounds are MMP-a 1 and muscular and reduce, the ball base [khu phosphorus which is (involucrin), pillar green (filaggrin) and enhance expression dose can be prophylactic clean (loricrin).

In the present invention resulting from the reduction of collagen wrinkle formation step, the reduction of the excessive collagen can be supplies said oxidative stress or ultraviolet irradiation.

In one embodiment of the present invention cosmetic composition according to skin ointment, cream, manufactured, nutritional skin water, pack, essence, hair tonic, shampoo, rinse, hair conditioner, hair treatment, gel, skin lotion, skin small [phu you, skin toner, astringent, lotion, milk lotion, feed [su department lotion, plant, massage cream, encapsulated into, feed [su department cream, hand cream, foundation, nutritional essence, sunscreen, soap, cleansing foam, cleansing lotion, cleansing cream, selected from the group consisting of body lotion and body cleanser but may have any number type, the number is not one. The number of required number number of cosmetic composition consisting each of these number type down-containing additive can be on various for number, amount and kind of these components can be uses for by one skilled hereinafter.

Cosmetic composition of the present invention number type paste, cream or when it is a gel carrier ingredient animal fiber, plant fiber, wax, paraffin, starch, space [thu coating, cellulose derivative, polyethylene glycol, silicon, bentonite, silica, talc or zinc oxide or the like can be used.

Cosmetic composition of the present invention this powder or spray when the number type carrier ingredient lactose, talc, silica, aluminum hydroxide, calcium silicate or polyamide powder can be used, in particular when the additionally chloro [...] spray, such as a butane or dimethyl ether can be - propane to expel the projectile.

In the case of cosmetic composition of the present invention solution or emulsion-solvent carrier ingredient number, solvating number or number is used device for cleaning, for example water, ethanol, isopropanol, ethyl carbonate, ethyl acetate, benzyl alcohol, benzyl benzoate, propylene glycol, 1, 3 - butyl glycol oil, glycerol aliphatic ester, polyethylene glycol or sorbitan fatty acid esters flow tides.

Cosmetic composition of the present invention number type suspension carrier ingredient when water, such as ethanol or propylene glycol liquid dilution number, box-ethoxylated alcohol, polyoxyethylene sorbitol esters and polyoxyethylene sorbitan ester such as suspension number, microcrystalline cellulose, aluminum meta [...], bentonite, agar or coating such as space [thu can be used.

Cosmetic composition of the present invention the interface number when the number type carrier ingredient aliphatic alcohol containing cleansing - active sulfate, aliphatic alcohol ether alkylsulfate, sulfonyl succinic ester, oh three motes this [thu which comes, imidazole [...] derivatives, methyl [...], cooperates with the disadvantages, fatty acid amide ether sulfate, alkyl polyglycoside, aliphatic alcohol, fatty acid glyceride, fatty acid d ethanol amide, vegetable oil, ethoxylated glycerol fatty acid ester can be used it teased derivative or the like.

Cosmetic composition of the present invention fluorescent materials, fungicidal number, hydrotropism inducing material, moisturizing number, direction number, direction number carrier, protein, solubilized number, sugar derivative, sunscreen number, vitamin, plant extract can be further including excipients like number.

In addition, the present invention refers to muscular alpha -, beta - muscular, gamma - (gartanin) compound of at least one compound selected from muscular and whether the tannin containing pharmaceutical compositions for preventing or treating skin wrinkle are disclosed.

Pharmaceutical compositions of the present invention pharmaceutically acceptable carrier, excipient or dilution number can be further includes a number. Pharmaceutical compositions of the present invention contained in a pharmaceutically acceptable vehicle number number as typically used, saline, sterile water, ringer's solution, buffer saline, lactose dextran solution, maltodextrin solution, glycerol, ethanol, lactose, dextrose, sucrose, sorbitol, mannitol, starch, acacia rubber, calcium phosphate, alginate, gelatin, calcium silicate, microcrystalline cellulose, polyvinylpyrrolidone, cellulose, syrup, methyl cellulose, methyl hydroxy benzoate, propyl hydroxy benzoate, talc, magnesium and mineral oils and the like acid including but, limited to are not correct. In addition said components number in addition to the antioxidant, buffer, bacteriostatic number, dilution number, number surfactants, binding number, lubricating number, wet number, number sweetener, flavor number, emulsion number, number or preserving number further comprises suspension such as can be. Pharmaceutical compositions of the present invention can be oral or parenteral administration, parenteral administration can be administered or a skin so as to method are disclosed. Pharmaceutical compositions of the present invention suitable dosage is number number of method, administration scheme, patient's age, weight, -, pathological conditions, food, administration time, routes, such as sensitive and reaction speed is determined by the various prescribed for a printed wiring board can be.

In addition, the present invention refers to muscular alpha -, beta - muscular, gamma - (gartanin) compound of at least one compound selected from muscular and whether the tannin containing improvement of skin wrinkle or antiaging health food composition are disclosed.

Health food composition of the present invention when used for food additives, either adding said second health food composition can be use with other food or food ingredients, can be suitable for use according to the conventional method. Mixing of active ingredient suitable for use in accordance with the aim of use german silver (preventing or ameliorating) can be. Generally, health food composition of the present invention hereinafter provided to maintain a number of morning fair food or beverage 15 parts by weight, preferably hereinafter are included in an amount of 10 parts by weight. However in the case of ingestion of the amount said health is hereinafter can have long said range, said range in number because the safety door insofar as the active ingredient can be used in a quantity.

Said special number smooth free type of health food. Said food health food composition can be added examples of meat, sausage, bread, chocolate, candy, snack, confectionery, pizza, if, other noodles, gum type, ice cream acid number a dairy product, various sprocket, beverage, tea drink number, such as alcoholic beverages and vitamin composite number may be, health food comprising both in conventional meaning.

In addition, health food composition of the present invention in food, in particular ethanol bath number can be disclosed. Number upon tank of the present invention functional food product includes ingredients which add to conventional ones, e.g., protein, carbohydrate, fat, nutrient and seasoning comprising the following number. For example, if in addition to the natural carbohydrate or flavor number number number bath to drink ingredient capable of further active ingredient. Natural the carbohydrate (e.g., glucose, such as fructose) said pathogenic agent mono, d company kara id (for example, liver, sucrose or the like), oligosaccharide, polysaccharide (e.g., dextrin, cyclodextrin or the like), or sugar alcohol (e.g., xylitol, sorbitol, such as erythritol) preferably in. The natural flavor (e.g., TAU staining, such as Stevia extract) on said flavor number number number (e.g., saccharin, aspartame or the like) can be using synthetic flavor.

Said health nutraceutical compositions in addition to various nutritional number, vitamin, electrolyte, flavor number, colored number, [pheyk the [thu it buys and salts thereof, alginic acid and salts thereof, organic acid, protective colloidal thickening number, pH control number, stabilizing number, antiseptic number, glycerin, alcohol, further number of calcium hydroxide can be used containing carbonated beverages. The triazines of the present invention the amounts of ingredients added to said large but not significant health nutraceutical compositions 100 parts by weight, 0. 01 To 0. 1 Parts by weight of generally is selected from the range are disclosed.

Hereinafter, the present invention broadcast receiver using more detailed in the embodiment. In the embodiment of the present invention is more specifically describe the present invention only these for the number one is a person with skill in the art that these range by not notified to the reduced in the art are disclosed.

[Material and method]

1. Muscular alpha -, beta - muscular, gamma - muscular and for separating and identifying whether the tannin compounds

Muscular fruit shell (7. 29 Kg) by extracting three times with ethanol extract of cervi 351g cream performed yesterday. In a seam sealing the reservoir said ethanol extract sequentially hexane, chloroform, ethyl acetate, butanol fraction order is performed hexane fractions (12. 3G), chloroform fractions (213g), ethyl acetate fractions (15g), butanol fractions (10g), water fractions (98g) obtained a. In one of the chloroform fractions using silica gel column chromatography (10 × 60 cm, 2000g, hexane (hexane) - EtOAc (100:0 to 2:1) and CHCl₃ - MeOH (20:1 to 1:1)) alpha - 10g performed a muscular obtained. The chemical structures of muscular alpha - having a high molecular mass and NMR analysis equipment value determined by means of the experiments (¹ And H¹³ C NMR chemical shift (ppm) of, coupling) was determined by comparing existing synthesized (also 1 and 2).

In addition, muscular fruit shell (1. 23 Kg) of ethanol extract by extraction using ethanol 3 times 87g leucorrhea needle embodiment are obtained. Said distilled ethanol extract after suspension for 1 to 3, chloroform, ethyl acetate, performed when the fraction of butanol, chloroform 50g hangover. Chloroform fractions silica gel column chromatography (5 × 95 cm, 832. 79G, chloroform: MeOH (100:0 to 0:100)) 18 which gives rise to two [...] (GMC-a 1 a-GMC-a 18) by Congress. GMC-a 6 (247 mg) HPLC (Phenomenex C18, 250mm × 10mm, 5 mm, 90% MeOH, 2 ml/min) may be changed to a muscular embodiment beta - (3. 5 Mg, tR 42. 0Min), whether the tannin (1. 5 Mg, tR 21. 4Min) was obtained, GMC-a 17 (960 mg) fractions MPLC (Medium Pressure Liquid Chromatography) (RP C provided 18, 120g, H₂ O-a MeOH (40 - 90%)) when the a embodiment, total 25 hypochondria (GMC-a 17 provided 1 a-GMC-a 17 provided 25) - positive number from his muscular 98 mg are obtained with a among these GMC17 provided 17 separating gamma. Beta, gamma - muscular and whether the tannin chemical structures of having a high molecular mass and NMR analysis equipment (gartanin) determined by means of the experimental value (¹ And H¹³ C NMR chemical shift (ppm) of, coupling) was determined by comparing existing synthesized (also 1 and 2).

2. Cell culture

HaCaT cells (human keratinous forming cell lines) is 37 °C, 5% of CO₂ Conditions, 10% of FBS (Fetal bovine serum) - DMEM (Dulbecco's Modified Eagle Medium) containing 1% of penicillin-resistant Streptococcus pneumoniae and Streptococcus feed (PS, Gibco) cultured in him.

3. Cell survival rate

The HaCaT cells (human keratinous forming cell lines) 24 hours according to a ultraviolet (UV Crosslinker, Ultra Lum) dilution and processing each sample concentration 20 mJ/cm² After 30 minutes to, MTS assay (CellTiter Aqueous One Solution Cell proliferation assay kit 3 - (4, 5 a-dimethylthiazol-a 2 a-yl) - 5 - (3 provided carboxymethoxyphenyl) - 2 - (4 provided sulfophynyl) - 2H-a tetrazolium, inner salt; MTS, Promega Co. Madison, WI, USA) microplate reader (Molecular Devices, Sunnyvale, CA, USA) in cell viability techniques 490 nm was measured by the testing.

4. MMP-a 1 and MMP-a 9 expression dose analysis

The HaCaT cells (human keratinous forming cell lines) 24 to process each time a transparent (UV Crosslinker, Ultra Lum) 20 mJ/cm² After the 30 minutes, the supernatant of a culture to collect MMP-a 1, MMP-a 9 ELISA kit (R&D Systems, Inc. , Minneapolis, MN, USA) using MMP-a 1 and MMP-a 9 expression dose were measured. The reagent may be performed quantifying the expression dose was [e tax it will reach over MMP-a 1.

5. Effective Pig conditions

The new variety of 6 male mouse (male HR provided 1, Hairless mice, Japan SLC Inc.) new week zero separated central effective after 1 purchased from adapting as well as during the main. The general condition during an adaptation period are observed to animal testing as well as healthy state. Breeding environment is 23 ± 3 °C temperature, humidity 50 ± 5%, contrast period 12 time (07:00 - 19:00/illumination time) was maintained. Polycarbonate (polycarbonate) between tester during effective cage (200 × 320 × 145 mm, Three-a shine Co. , Daejon Korea) military north korean workers party to have 5 fed mary, dedicated feed Harlan 2018S mouse feed (Harlan^TM , USA) when the free machine for livestock, water glass disinfecting constant readings was free machine for livestock. Sample administration controls (control), group with UV radiation (UVB), alpha - (α-a mangostin) total 3 divided into two muscular administration group the group consisting of morphine. Sample using administration of oral treatment with respect mouse was administered. The total dosing period 12 mainly during main 5 was administered.

6. Ultraviolet irradiation

12 Main 3 times during population number [...]-capturing ultraviolet irradiation experiments have main kind of embodiment, a UVB ultraviolet lamp (Mineralight UV Display lamp, UVP, USA) was used. The ultraviolet irradiation and daytime 60mJ/cm2 is 1 - 6, 7 - 12 in which main 12 90mJ/cm2 during visit from the police. The operating using ultraviolet irradiation (Delta OHM, Italy) for measuring the amount of light, illumination times can be regulated.

7. Skin histological observation

In order to identify wrinkle billion number efficacy, mixed with an aqueous 10% neutral formalin solution after each experiment group dermal tissue fixed flushing, dehydration, transparent, then the penetration process has a thickness of 4 micro m fragment formed in paraffin and produced, Hematoxylin (H&E) dyeing and Masson's trichome dyeing his & Eosin embodiment. H&E dyeing keratinous layer embodiment texture bath using a microscope mounted to epidermal cells basement membrane thickness (epidermal basement membrane) were measured.

8. In dermal tissue RNA extraction and quantitative PCR

RNasy Mini kit (Qiagen, Valencia, CA, USA) to each experimental group with an aqueous skin tissue RNA extracted from the a. CDNA synthesis is High Capacity cDNA Reverse Transcription Kit (Applied biosystems, USA) according to his manual of the radiation over a number of branches. For each sample of RNA synthesized using micro g 2 gene was PCR using a Taqman probe assay gene for expression of VEGF-embodiment. Each PCR primer (Involucrin, Filaggrin) internal control using the Taqman probe when the experiment using the β a-actin expression amount was.

9. Electrophoretic and the [wey the [su it shook off [...] (Western blotting)

20 Mg of dermal tissue out using proteins selected from the, reservoir and to isolate the proteins, matching opening the same amount of protein (actin) moved from PVDF membrane by reacting protein expression dose after electrophoretic antibodies were measured.

10. Statistical method

The result obtained in the present invention one a-way ANOVA using controls and significance between experiments on Student t-a test group was assay (<p 0. 05).

In the embodiment 1. Alpha -Muscular, Beta -Muscular, Gamma -Muscular and Whether the tannin According to cells for treating chemical compound Survival rate Analysis

The HaCaT cells (human keratinous forming cell lines) 24 hours each sample (alpha - muscular, muscular beta -, gamma - muscular and whether the tannin) by a concentration (1. 0, 2. 5, 5. 0, 10 Μm) treating, a ultraviolet (UV Crosslinker, Ultra Lum) after 30 minutes, MTS assay (CellTiter Aqueous One Solution Cell proliferation assay kit 3 - (4, 5 a-dimethylthiazol-a 2 a-yl) - 5 - (3 provided carboxymethoxyphenyl) - 2 - (4 provided sulfophynyl) - 2H-a tetrazolium, inner salt; MTS, Promega Co. Madison, WI, USA) microplate reader (Molecular Devices, Sunnyvale, CA, USA) in cell viability techniques to 490 nm were measured.

The disclosure also result as 3, cell survival rate to about 51 by UV radiation. Have reduced to 7%, to process the present invention according to when, ultraviolet irradiation group cell survival rate compared to fabrics. In particular, in the case of muscular alpha -, ultraviolet rays are irradiated substantially similar levels of survival rate not nothing control nine and mistletoe.

In the embodiment 2. Alpha -Muscular, Beta -Muscular, Gamma -Muscular and Whether the tannin Compound according to collagen degrading enzymes (MMPs) Expression dose reduction of confirmation

Collagen degrading enzymes (MMPs; matrix metalloproteinases) is involved in extracellular matrix and basement membranes by decomposition, reduced collagen affecting enzyme are disclosed.

The, involved in MMP-a 1 and MMP-a 9 expression dose of skin-mail, of the present invention muscular alpha -, beta - muscular, gamma - whether the tannin compounds whether or improving muscular and therefrom. As a result by UV radiation and ultraviolet rays are irradiated cells to MMP-a 1 MMP-a 9 expression dose of the present invention increased significant advantages over both the muscular alpha -, beta - muscular, gamma - whether the tannin compounds muscular and when processing, MMP-a 1 and are separated from MMP-a 9 expression dose has been confirmed (4 also).

In the embodiment 3. Alpha -Of muscular Processing according to skin changes of organization analysis

Ultraviolet light derived new wrinkles mouse army dermal tissue in an animal model was mixed with an aqueous H&E dyeing and Masson's trichrome dyeing embodiment.

The results of the H as a disclosure to also 5A&E dyeing, ultraviolet irradiation ultraviolet group compared to controls finish, braided with one another closer to the stratum corneum, epidermal thickness increased value can be confirmed, alpha - group consisting in administering the stratum corneum have been relaxed muscular epidermal thickness decreases were confirmed. The disclosure also 5B Masson's trichome even a dyeing result, in the four corners from the group consisting of the stratum corneum has been irradiated with ultraviolet rays, but collagen fiber is not observed, α - administering ultraviolet light irradiation increases compared to the group from the group consisting of muscular collagen fiber has been confirmed.

In addition, H E dyeing keratinous layer embodiment texture&thickness measured using a microscope mounted to epidermal basement membrane or bath, ultraviolet irradiation from the group consisting of epidermal thickness was increased, the group consisting of muscular alpha - administering reduces the thickness of the skin has been confirmed that the schedulable (also 5C).

In the embodiment 4. Alpha -Muscular Processing according to MMP-1 And MMP-9 Protein expression dose change analysis

MMP-a 1 protein plays an important role in skin aging wrinkle formation known as MMP-a 9 expression dose measured ultraviolet irradiation and animal skin tissue without applying ultraviolet ray (UVB) protein expression dose group compared to the group has been increased formate, the muscular administration in the group consisting of alpha (α-a mangostin) compared - been reduced protein expression dose (6 also).

In the embodiment 5. Alpha -Muscular, Beta -Muscular, Gamma -Muscular and Whether the tannin Compound treatment according to identify skin moisturizing effect

The ball base [khu phosphorus which is (involucrin), pillar green (filaggrin) and prophylactic skin moisturizing function and it acts as a barrier preventing damage to clean (loricrin), ultraviolet damage amount expressed by substances known.

The use of RNA in tissues by extracting the ball base [khu phosphorus which is in in the embodiment 5 (involucrin), pillar green (filaggrin) and prophylactic clean (loricrin) expression dose confirmed result, ultraviolet (UVB) group consisting in the ball base [khu phosphorus which is (involucrin), pillar green (filaggrin) and prophylactic clean (loricrin) expression dose decreases muscular formate alpha -, beta - muscular, gamma - whether the ball base [khu phosphorus which is ultraviolet irradiation group in compound treated group compared muscular and tannin (involucrin), increased expression of RNA by pillar green (filaggrin) and prophylactic clean (loricrin) has been confirmed (also 7).