FUNCTIONAL ANTIOXIDANT FOOD COMPOSITION COMPRISING CELOSIA CRISTATA L. FLOWER EXTRACT FERMENTED BY LACTIC ACID BACTERIA AND PREPARATION METHOD THEREOF
The present invention refers to edible cockscomb flower [...] lactic acid fermentation broth containing antioxidative functional food composition relates to search, adding distilled water to give a cockscomb acutum and flower dry powder is provided, said lactic acid fermentation characteristics in the extract which it will bite cockscomb flowers having high temperature short time inoculation, lactic acid fermentation obtained by fermentation broth containing human antioxidant food composition for manufacturing method and to increasing activity are disclosed. Cockscomb (Celosia cristata L.) (Amaranthaceae) 1 year lifestyle center character neck and plant genome is amaranth belonging to damage from cold weather grass flesh as ornamental and cultivation product has, been used by traditional anger cockscomb flowers [...] about number into the tablet. Stem and leaf represents the hemorrhoids, heterogeneous, hematemesis, microscopy, [...], and the seed is a sheep [hyel and hemostatic efficacy of treating trihydroxyheptanoic, melena, heterogeneous, soy sauce bottle, eye disease treating the appearance of the known. In particular, the flowers and hemostatic efficacy of sheep [hyel, due to discharging blood provider, heterogeneous, hematemesis, hemoptysis, [hyel rim and treating the appearance of the known. Cockscomb ingredients include beta-a cyanin, kaempferitrin, amaranthin, known pinitol and the like, a large amount of potassium nitrate and of flavonoid known 5 on new isoflavone in cristatein (5 a-hydroxy-a 6 a-hydroxy n-methyl-a 7, 2' - demethoxyisoflavone) containing phenolic plant orgin and widely present various pharmacologically, biological activity and antioxidant action is known. Natural generally relates to vitamin, mineral, dye and various characteristics of components important nutrients abundantly resistance by maintaining health, improving, diseases significantly contributing known. However inhibits toxicity to the package processing techniques to 10sup16. increasing number and a stand-alone functional cooled and of active ingredients. Inoculating the fermentation microorganism food polymer materials and break into small molecules and beneficial to symbol has an for fire-fighting, new flavor degradation and rear long period, recent studies are generated physiologically active active principle with fermented food etc. than the aluminum. The lactic acid fermentation lactic acid (lactic acid) (Lactic acid bacteria) generates a large amount of energy are obtained sugars, sugar metabolic product consumed 50% or more without benefit generated close a bacteria are disclosed. The gram positive bacteria include an effective amount of lactic acid or more anaerobic microorganisms and motility and is predominantly car mask oh voice number growth and penetration is performed multi vitamin, amino acid, peptide or C.. The lactic acid digestion of food ingestion of intestinal microorganisms generally, absorbent, such as inducer of beneficial bacteria helps for a printed wiring board as to functions and extinguishing machine exhibits increased intestinal disease (Rolfe, 2000, J Nutr, 130:396 - 402) effect. In addition, the synthesis of the utilised via fermentation by lactic acid nutrition and health effects, acting door lock handle, immunoadjuvant, improving cirrhosis of the liver, after, skin care effect etc. nutritional effects the number of passes is reported. The present invention associated with a compensation as prior art patent number 10 - 0881035 virus containing human diseases due to organic solvent extract cockscomb call is registered for the prevention and treatment a composition, of an organic solvent extract cockscomb the call registration patent 10 - 0881033 prophylaxis and treatment of diseases due to a animal virus composition for publicly known in the nanometer range. However to date cockscomb fermented lactic acid fermentation characteristics only week inoculation in the extract which it will bite material having high temperature short single flower antioxidative functional and functional to form positioned under a publicly known techniques have free. The purpose of the invention the anti-adhesive properties in the extract which it will bite a flower of a single raw material cockscomb hot short fermentation Another object of the present invention said active ingredient containing lactic acid fermentation antioxidative functional and a functional flower [...] cockscomb it to food composition or a reversed F. [...] number. It is an object of the present invention said dry powder of 15 times corresponding to the weight of the tank by a cockscomb flower is immersed in distilled water and; high extraction condition antioxidant activity in the range of 1 - 3 time most preferably 60 °C 30 provided 90 °C 2 the extraction which will bite in time steps; said flower having GRAS (Generally Recognized As Safe) lactic acid fermentation characteristics in the extract which it will bite cockscomb hot short class Cockscomb fermentation lactic acid bacteria of the present invention significantly promoted flower [...] antioxidation effects which, using only natural substances toxic to human body since no side effect such as an air-polished available functional effects as well as, non-organic acid by fermentation temperature and tin-free fermentation number [...] short already excellent flow tides. Figure 1 the present invention according to a flower of a lactic acid fermentation broth of coarse process diagrams indicating cockscomb [...] of manufacturing method are disclosed. Figure 2 shows a graph representing the result of measurement according to the present invention according to the extraction which will bite on each DPPH radical active erase cockscomb flower also are disclosed. Figure 3 shows a cockscomb total polyphenol content according to the present invention according to flower also for the extraction which will bite on each graph representing the result of measurement are disclosed. Figure 4 the present invention flower lactic fermentation broth of DPPH radical by inoculation in the extract which it will bite cockscomb prepared by the number according to erase siberian bustard antioxidant activity of graph representing the result are disclosed. Figure 5 the present invention [...] cockscomb prepared by the number of total polyphenol content according flower lactic acid fermentation activity graph representing the result of measurement are disclosed. The present invention refers to a flower extract fermented lactic acid which reduces number after dressing and flower cockscomb cockscomb flower effect increasing activity and anti-aging comprising lactic acid fermentation broth and cockscomb produced therewith [...] functional food composition number [...] substrate. The present invention can be used according to the preferred lactic acid bacteria In addition, the present invention according to the manufacturing method in the case of lactic acid fermentation composition (a) flower [...] cockscomb cockscomb fermentation active material biocompatible or dried cockscomb flower can be used a few flowers. (B) adding a cockscomb cockscomb chuck 15 times the extract which it will bite a flower of a flower; 30 provided 90 °C in time 1 - 3, most preferably 60 °C 2 comprising extracting can be in time. (C) obtained in said step a flower in the extract which will bite the cockscomb separated from each other by 1% (w/w) contrast medium composition to be inoculated with a lactic acid bacterium cultured pre-both grudges entire glucose 0 - 5% starch liquid after, further adding yeast extract 0 - 5% comprising the following steps. (D) said a) and b) after mixture The present invention refers to in addition, lactic acid fermentation broth of flower cockscomb DPPH radical erase active, evaluating total phenol content that has been improved antioxidant activity through multiple myelomas are included. In addition, the present invention refers to lactic acid fermentation liquid lactic acid fermentation liquid of antioxidation effects cockscomb flowers is contained in number [...] other lactic acid bacteria counting result. Hereinafter, in the embodiment of the present invention specific content for example detailed as follows. In the embodiment of the present invention to provide a range for the present invention is exemplified is limited to rights are not correct. In the embodiment 1. The present invention flower number bath cockscomb rheumatoid arthritis The present invention used to grind a cockscomb flower is dried using but a cockscomb, shaft tube can be used. Flower and flower powder weight of 15 times the extract which it will bite cockscomb dried cockscomb (w/w) extracted from the time corresponding to 60 °C by adding 1 - 3 in distilled water. 2 60 °C extracting antioxidant activity and total polyphenol content in time when ever (also 2 and 3 also). Filtering the filtrate recovered through pressure after extraction as well as flower cockscomb acutum cold rolled material. In the embodiment 2. Cockscomb [...] flower number bath lactic acid fermentation broth The X-axis obtained in flower is cultured in the extract which it will bite cockscomb material separated from each other by said in the embodiment 1 Experiment example 1. Antioxidant activity by DPPH siberian bustard erase (Α, α-a diphenyl-a β-a picrylhydrazyl) DPPH center nitrogen in compounds of a radical (radical) is stabilized to the stabilized radical oxidation or erase function according to the present can be activity. In the present invention DPPH radicals such as the measurement of the potency Biosis (1958) were measured by the method of. In the embodiment 2 and 0 each sample obtained in said in the embodiment 1. DPPH 5 ml to 5 ml into a room, and expressions 1 to 30 minutes in place by reacting 517 nm measured in absorbance is inhibited by the rate the bill. As well as a positive controls include vitamin C. Experiment result, antioxidant activity by DPPH you will in Figure 4 in the embodiment 1 in the embodiment 2 of a flower of a sample of erase siberian bustard cockscomb rheumatoid arthritis compared selection increases oxidation or active lactic acid fermentation erase DPPH looking up. According to the present invention most preferably yeast extract 5% glucose 5% lactic acid fermentation cockscomb prepared by the number flower [...] added lactic acid fermentation in a medium and a high temperature short fermentation characteristics by fermentation with 30% or more but enhanced antioxidant activity, said 5% addition of one number range to the intensive and not the. Experiment example 2. The total polyphenol content antioxidant activity Total polyphenol content were measured for instance Folin non-Denis (Zhou et al. , 1980). 1 Ml of each sample to said in the embodiment 1 in the embodiment 2 and 3 minutes after applying 1 ml 10% Na2CO ₃ 1N Folin non-Ciocalteu's reagent 1 ml being the blended solution by applying it to a total polyphenol content after 1 700 nm at room temperature being the time stored in the evaluated in absorbance. Experiment result, total polyphenol content in the embodiment 1 in Figure 5 you will flower cockscomb of lactic acid fermentation sample has been expanded in rheumatoid arthritis as compared in the embodiment 2 according to sample. According to the present invention comprises glucose 5% lactic acid fermentation [...] cockscomb prepared by the number flower, yeast extract 5% lactic acid fermentation characteristics the present invention in a medium added with 65% antioxidant activity by fermentation include saw but hot short, addition of the number range are not necessarily 5% to one and not the case. Experiment example 3. PH and lactic acid bacteria counting In the embodiment 2 of the present invention said in the embodiment 1 with the extract which it will bite cockscomb lactic acid fermentation composition obtained in flower cockscomb obtained in flower [...] analyzes the sample for counting lactic acid pH 0 in the embodiment 2. 85% After 10 times dilution MRS agar medium dilution method in sterile saline to continuous measuring method colony possibility which is inoculated into his timer is lactic acid. Experiment 1 in the embodiment 1 sample is studied at pH 5 resulting in a desired bright. 02 And, in the embodiment 2 sample pH is According to the present invention Number 1 attainments. Cockscomb flower lactic acid bacteria fermented beverage number bath According to the present invention in the embodiment 2 obtained lactic acid fermentation is contained beverage such as high pressure liquid coolant to table 2 cockscomb flower [...] his number. According to random number for each male and female attainments said number into a functional inspection panel 5 name extracted beverage produced therewith his embodiment. With the extract which it will bite his flower cockscomb enable all contrast. Apparatus and method for point 7 functional assay measures have, for table 3 experiment corresponded such as disclosed. Experiment result color, taste, in contrast sphere or experiment compared to opening 2 total preference The present invention refers to as the device obtaining the flower cockscomb adding lactic acid fermentation characteristics acutum from nutritional inoculum is provided which produces high temperature short with pH 3. 9 Hereinafter anti-oxidative activity of the fermented food preference calculation using fermentation and to form excellent [...] number effective health functional food industry in feed invention are disclosed. The present invention relates to a method for preparing a natural fermented composition having improved antioxidant and immune activities by fermentation of a Celosia cristata L. flower extract with lactic acid bacteria and, more specifically, to a method for preparing a Celosia cristata L. flower extract fermented with lactic acid bacteria, comprising adding extra nutrients to a Celosia cristata L. flower extract, and inoculating and fermenting a fermentation strain Enterococcus sp. or Lactobacillus sp. lactic acid bacteria strain at high temperature in a short time, and to a functional antioxidant food composition using the same. COPYRIGHT KIPO 2018 Plant obtained acutum and; lactic acid bacterium obtained in said step of vaccination in the extract which it will bite and; through a [...] lactic acid fermentation broth of lactic acid fermentation processes in manufacturing method comprising the steps of, said chuck in the extract which it will bite of plants flower powder by adding 60 °C cockscomb [...] obtained by extracting and time 2, the lactic acid bacteria Back number Back number In accordance with Claim 1 number bath to pH 3. 9 Hereinafter characterized in flower [...] cockscomb lactic acid fermentation broth Cockscomb [...] flower rays to lactic acid fermentation according to Claim 4 enhanced gastrodia root characterized food compositionPH before fermentation After fermentation pH Culture viable count 5. 02 3. 82 1. 6 X 109 Cfu/ml 5. 02 3. 54 1. 3 X 109 Cfu/ml Material name [...] beverage extract Cockscomb [...] flower of a lactic acid bacterium (wt %) 10. 0 An oligosaccharide solution (wt %) liquid 12. 0 Dextrin (wt %) 0. 5 (Wt %) citric acid 0. 13 (Wt %) lactic acid calcium 1. 6 Vitamin C (wt %) 0. 03 C [...] sodium (wt %) 0. 02 The gel the sword which burns (wt %) 0. 03 Be positive number (wt %) Remaining (Wt %) total 100. 0 Classification color taste Total preference Matching opening 4. 5 4. 4 4. 6 Experiment 1 opening 5. 8 6. 2 6. 1 Experiment 2 opening 5. 1 5. 1 4. 9
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