COMPOSITION FOR IMPROVING MEMORY CONTAINING TENEBRIO MOLITOR EXTRACT

Side effects risk lower than in the present invention, derived from pharmaceutical compositions consisting of excellent natural to perform her.

In the present invention, box fed to-sized boiler (Tenebrino molitor, Mealworm) extract pharmaceutical compositions where ability has been confirmed.

I.e., in the embodiment of the present invention tested in the [su the nose pawl it pushed to one of cordyceps militaris fed mouse impairing of water stored beverage exhibits high brown arylpyrazole profiteering [su have maze test is carried out, to brown arylpyrazole extract is fed cordyceps sinensis is superior in pharmaceutical compositions for confirming the first call request.

Thus, in one aspect the present invention refers to, to-sized boiler is fed cordyceps sinensis (Tenebrino molitor, Mealworm) containing extract composition for pharmaceutical compositions are disclosed.

In the present invention, said box fed to-sized boiler (Tenebrino molitor, Mealworm) to-sized boiler is fed cordyceps sinensis extract (Tenebrino molitor, Mealworm) after pigments and a, organic solvent or water can be achieved by extracting. Said organic solvent ethanol, methanol, butanol, propanol such as polar solvent; hexane, ether, benzene, such as toluene in a nonpolar solvent; chloroform, ethyl acetate, acetone, methylene chloride intermediate polar solvent such as example but, limited to are not correct.

Said box fed to-sized boiler (Tenebrino molitor, Mealworm) extract in water or in an organic solvent such as ethanol to enhance the output box fed-sized boiler (Tenebrino molitor, Mealworm) immersing, pottery, is distilled to extract or can be extracted with supercritical fluid extraction or microwave extraction, filtration and concentrate can be obtained through process.

On the other hand, pharmaceutical compositions containing as signal immunity to-sized boiler is fed with the capability (Tenebrino molitor, Mealworm) when seeds extract, pharmaceutical compositions containing active ingredients separated fractions containing each Internet predicted.

In the embodiment of the present invention to-sized boiler is fed in one of cordyceps sinensis (Tenebrino molitor, Mealworm) extract each hexane, chloroform, ethyl acetate, butanol and water each fraction may be sequentially obtains, with pharmaceutical compositions containing active fractions obtained employed for conducting experiments. As a result, make sure that the ability better pharmaceutical compositions containing water are respective fractions were.

According to the polarity of the solvent organic fractions THon said separating dissolved compounds can be acquiring conventional method, in the present invention to improve reliability and soft leaf extracts of the extracted first soft root hexane, chloroform, ethyl acetate, butanol and water fraction to hexane fractions, chloroform fractions, ethyl acetate fractions, butanol fraction of water and fractions yesterday.

In the present invention in addition, pharmaceutical compositions containing capacity most excellent conducting pharmaceutical compositions containing fractions identified target chromatography for separation and identify predicted active body employed for each Internet Company.

In the embodiment of the present invention the electrolyte of chromatography (MPLC) one, (TLC) and high-performance liquid chromatography (HPLC) thin film chromatography employed for separating active body by performing pharmaceutical compositions, electron impact mass spectrometry (EI-a MS), nuclear magnetic resonance (NMR) and DEPT (Distortionless Enhancement by Polarization Transfer) employed for identifying active body were performed pharmaceutical compositions.

Thus, the present invention refers to in another aspect, to-sized boiler is fed cordyceps sinensis (Tenebrino molitor, Mealworm) separated from extract having an active material and a method for isolating a pharmaceutical compositions are disclosed.

The present invention according to pharmaceutical composition to-sized boiler is fed cordyceps sinensis (Tenebrino molitor, Mealworm) bark extract and rotates about number in addition to therapeutic active agents using physiologically acceptable auxiliary number number can be tank, said auxiliary number number include excipients, disintegrating number, sweetener number, number coupled, coating number, expansion number, lubricating number, number [...], flavor number can be use.

Said pharmaceutical compositions containing the active composition for administration in addition to said substrate further comprising at least one dermatologically acceptable carrier about number 1 therapeutic composition can be preferably under anger about number number number.

Number number number number of form about said granular compositions, acid number, positive number, coated tablets, capsule number, number left, number solution, syrup, juice, suspension number, oil number, number or injectable liquid droplets can be like number. For example, the form number number according to the number of positive number or capsules, effective component is ethanol, glycerol, water such as oral, about number can be combined with a non-toxic dermatologically acceptable inert carrier. In addition, desired or if necessary, a suitable binding number, lubricating number, number of the leaving group can be in admixture number in addition disintegrating. A suitable binding number is the number one is but not limited to, starch, gelatin, glucose or beta - natural sugar such as lactose, corn sweetener number, acacia, such as sodium or [...] the [ley which comes the [thu and natural and synthetic gum, sodium stearate, magnesium stearate, sodium benzoate, sodium acetate, sodium chloride etc.. The disintegrating number is the number one but not limited to, starch, methyl cellulose, agar, it cuts the toe knit, etc. and xanthan gum. Liquid solution which number number of acceptable carrier include therapeutic composition about number, sterilization and biocompatible provided, saline, sterile water, ringer's solution, buffer saline, albumin injection solution, dextran lactose solution, malto dextrin solution, glycerol, ethanol and of these components can be mixed at least 1 component, antioxidant number needed, buffer, addition of other usual number such as bacteriostatic number can be added. In addition dilution number, dispersion number, number surfactants, number and lubrication number added coupled added to aqueous solution, suspension, emulsion for injection such as number type, pill, capsule, granule or positive number to number number can be under anger.

In addition, said pharmaceutical compositions containing said substrate in addition to other active composition for use with food or food component further can be, according to the conventional method can be suitably. According to intended use (prevention, health or therapeutic treatment) german silver method for effective mixing can be determined. Generally, when food or beverage of the present invention composition provided to maintain a number of tank 15 weight % hereinafter, preferably in an amount of 10 weight % hereinafter are included. However, health and hygiene purposes or in the case of ingestion of said range and can have a long term health control purposes hereinafter, door number because as the active ingredient in the aspect of safety insofar as an amount of at least said range can be also be used.

Said type of food is simplified and a smooth free number. Adding said food products include meat, sausage, bread, chocolate, candy, snack, confectionery, pizza, if, other noodles, gum type, ice cream acid number a dairy product, various sprocket, beverage, difference, drinkable number, alcoholic drinks and vitamin composite number and the like, health food comprising both in conventional meaning.

When beverage compositions of the present invention are the pharmaceutical compositions containing, lower number or natural carbohydrate ingredient is like conventional beverage flavor can be added. The above-described natural carbohydrate glucose, oligosaccharides such as mono pathogenic agent, liver, such as d company kara id comprising sucrose, and dextrin, cyclodextrin such as natural sweetener number or, saccharin, oh [su group [lu with burning, can be use such as synthetic sweetener number. The ratio of said natural carbohydrate of the present invention per 100 ml composition generally about 0. 01 to 10g, preferably about 0. 01 to 0. 1g are disclosed.

In addition to said nutritional composition includes number of the present invention, vitamin, electrolyte, flavor number, colored number, [pheyk the [thu it buys and salts thereof, alginic acid and salts thereof, organic acid, protective colloidal thickening number, pH control number, stabilizing number, antiseptic number, glycerin, alcohol, number of calcium hydroxide or the like can be used in carbonated beverages. In addition to of the present invention composition comprises natural fruit juice, fruit juice beverage and vegetable beverages can be pulp of number for numerical control machine. These components can be used in combination or independently. The triazines of the present invention composition 100 parts by large number but not significant added ratio of 0. 01 to 0. 1 parts by weight of generally is selected from the range are disclosed.

[In the embodiment]

Hereinafter, the present invention broadcast receiver through more detailed in the embodiment. The present invention is to provide a galvanic these in the embodiment is exemplified, in the embodiment of the present invention interprets these range number by one person with skill in the art-case is not in the in the art to will.

In the embodiment 1 :-sized boiler extraction

1 - 1 :-sized boiler breeding

Brown well! low interest conventional laboratory conditions i.e., temperature 25 ± 1 °C, relative humidity of 50 ± 5% Seoul university pus lifestyle unit secretion was fed chamber. Cordyceps food stuff is capable of wheat bran, composite vitamin class by mixing the molecules was hereinafter, cabbage leaves his [...] number 1 work 4 - 5 times.

1 - 2 :-sized boiler extraction

In the embodiment 1 - 1 in breeding to-sized boiler into a body drier to (50 °C) in ethanol at room temperature during 3 48 3 times to 90 L e [sway crossroad pigments and drying time intervals is immersed in filtration (Whatman no. 2, Maidstone, UK)) after, rotating with vacuum condenser ethanol extract filtrate is heated and concentrated to-sized boiler is obtained.

Experiment example 1: ethanol extract (scopolamine) induced mouse pharmaceutical compositions containing force confirming the [su the nose pawl it pushed-sized boiler

Pharmaceutical compositions used for test animals ICR female mouse-effect assay (25 provided 30g) to polyacrylic cage (38 × 23 × 10cm) 12:12 light period sufficient to feed and water while managing to 10 per embryo [...] his number. Test animal management of test animal ethical reference (NIH publication No. 85 - 23, revised 1985 years) and alloy of animal experiments for observing his university ethical guidelines. Mouse 10 by least two groups 6 are respectively individual [controls, the [su the nose pawl it pushed (scopolamine) group, -sized boiler ethanol extract processing group (60 mg/kg and 100 mg/kg), ascorbate [...] processing group (10 mg/kg), indoline csfv processing group (10 mg/kg)] profiteering [su daily divided into 1 time of the water maze test his embodiment of the result. Extract as memory impairment, csfv [...] ascorbate or the processing 30 minutes the [su the nose pawl it pushed (1 mg/kg, i. P) is induced. Controls are 0. Each individual in accordance with the weight 9% saline only 0. 02 ml/g was in. Comparison between average processing (P=0. 05) is the sample perot [ni law using the air.

Labyrinth (Harvard Bioscience, Holliston, Massachusetts, USA) profiteering [su water (90 cm diameter, circular water height 45 cm) filled with water walls with 25 cm to 24 provided 25 °C SiO been filling. (Diameter: 10 cm, height: 23 cm) 4 black support one of the above minute sleep 2 cm below a water been secured such that see with the naked eye. Experimental work are wound around the first supporter for 60 seconds so as to escaping mouse experiments can swim. Mouse 60 seconds if 10 seconds does not stand thereof in English on stand thereof decreased. Embodiment 20 seconds before the next training out water and rest-shaking. This process has been 5 times daily. The daily 5 to 60 seconds with respect to the mouse experiments where training is training test 5. After training, learning pharmaceutical compositions containing water escape through a different group of mouse maze inspection profiteering [su moving distance, time and speed to result shown and also 1 - 3.

As shown in the fig. 1, is a distance of the [su the nose pawl it pushed alone treatment group (0. 5 mg/kg, i. p.) compared to the saline group are processed [...] platform movement but longer distance, larvae extract well! low interest, they are groups as compared to the [su the nose pawl it pushed the phosphorus csfv [...][...] ascorbate or shorter travel alone processing group mistletoe.

Also as shown in the variation 2, the [su the nose pawl it pushed induction group are processed during which 5 exit [...] compared to saline group the longer delay time between tester labyrinthine and viscoelasticity. However well! low interest extract (100 mg/kg) or positive matching job [...] ascorbate (10 mg/kg) and lean csfv (10 mg/kg) treated group as compared to the [su the nose pawl it pushed together and the [su the nose pawl it pushed much shorter delay time group alone and viscoelasticity.

As shown in the 3 also, in one aspect the [su the nose pawl it pushed in WDM-based assays are processed [...] group compared to the saline group processing alone has been slow. However, well! low interest extract (100 mg/kg) or positive matching job [...] ascorbate (10 mg/kg) and lean csfv (10 mg/kg) alone as compared to the [su the nose pawl it pushed the group they are groups [...] a faster processing speed based assays were confirmed.

The present invention techniques that are directionally-specific content portion more precisely bar, the party industry to only a preferred embodiment and procedure only in person with skill in the art such in particular aspect, the range of the present invention apparently is the number that are not one will. The, substantial range of the present invention defined by appended claim and their equivalent that will.