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Небесная энциклопедия

Космические корабли и станции, автоматические КА и методы их проектирования, бортовые комплексы управления, системы и средства жизнеобеспечения, особенности технологии производства ракетно-космических систем

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Мониторинг СМИ

Мониторинг СМИ и социальных сетей. Сканирование интернета, новостных сайтов, специализированных контентных площадок на базе мессенджеров. Гибкие настройки фильтров и первоначальных источников.

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Применить Всего найдено 6103. Отображено 100.
19-01-2012 дата публикации

Methods of forming and using a solid-phase support

Номер: US20120012250A1
Автор: Aaron Jones, Brett Ellman, David Heiner, Michal Lebl, Steve Fambro
Принадлежит: Illumina Inc

Disclosed herein are methods of method of making a substrate for performing a chemical synthesis reaction.

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Номер записи: 1
23-02-2012 дата публикации

Microwave Assisted Chemical Synthesis Instrument with Controlled Pressure Release

Номер: US20120043313A9
Автор: Edward Earl King, James Edward Thomas
Принадлежит: Individual

An instrument is disclosed for carrying out controlled microwave assisted chemical processes, and that is particularly useful for handling relatively small samples. The instrument includes a needle, and a needle seal adjacent the needle and having a shaft coaxial with the needle and in communication with the needle. A pressure transducer is opposite the needle seal from the needle and in communication with the shaft. A housing holds the needle to the needle seal and the needle seal to the transducer. A chamber is formed between the housing and the needle seal. A lateral shaft goes through the needle seal from the coaxial shaft to the chamber in the housing, and a valve is in communication with the chamber.

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Номер записи: 2
31-05-2012 дата публикации

Multi-Shell Microspheres With Integrated Chromatographic And Detection Layers For Use In Array Sensors

Номер: US20120135396A1
Автор: Adrian Goodey, Dean P. Neikirk, Eric Anslyn, Jason Shear, John T. McDevitt
Принадлежит: University of Texas System

The development of miniaturized chromatographic systems localized within individual polymer microspheres and their incorporation into a bead-based cross-reactive sensor array platform is described herein. The integrated chromatographic and detection concept is based on the creation of distinct functional layers within the microspheres. In this first example of the new methodology, complexing ligands have been selectively immobilized to create “separation” layers harboring an affinity for various analytes. Information concerning the identities and concentrations of analytes may be drawn from the temporal properties of the beads' optical responses, Varying the nature of the ligand in the separation shell yields a collection of cross-reactive sensing elements well suited for use in array-based micro-total-analysis systems.

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Номер записи: 3
05-07-2012 дата публикации

Multi-well rotary synthesizer

Номер: US20120171088A1
Автор: Daniel W. Hugens, Gary R. McLuen, Richard J. Hanney
Принадлежит: McLuen Design Inc

An apparatus for synthesizing polymer chains includes a controller, a plurality of precision fit vials circularly arranged in multiple banks on a cartridge, a drain corresponding to each bank of vials, a chamber bowl, a plurality of valves for delivering reagents to selective vials, and a waste tube system for purging material from the vials. A purging operation can be selectively performed on one or more of the banks of vials. The plurality of vials are stored in the cartridge and are divided among individual banks wherein each bank of vials has a corresponding drain. There is at least one waste tube system for expelling the reagent solution from vials within a particular bank of vials when the waste tube system is coupled to the corresponding drain. The cartridge holding the plurality of vials rotates relative to the stationary banks of valves and the waste tube system.

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Номер записи: 4
19-07-2012 дата публикации

Methods for screening and arraying microrganisms such as viruses using subtractive contact printing background

Номер: US20120184458A1
Автор: Daniel J. Solis, Emmanuel Delamarche, Sean R. Coyer
Принадлежит: International Business Machines Corp

Methods for screening and arranging microorganisms such as viruses in an array using subtractive contact printing are provided. In one embodiment, a method for forming an array of receptors for microorganisms comprises: patterning an array of structures on a first substrate to form a template on a surface of the first substrate; applying a receptor material to a face of a second substrate; and contacting the face of the second substrate with the template to remove a portion of the receptor material from the second substrate, thereby forming an array of receptors on the second substrate.

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Номер записи: 5
02-08-2012 дата публикации

Apparatus and methods for microfluidic applications

Номер: US20120195809A1
Автор: Douglas Roy, Joel Fearnley, Peter Ghazal, Stuart Polwart
Принадлежит: AGILENT TECHNOLOGIES INC

Non-rigid tape apparatus and fabrication methods for microfluidic processing applications such as gel electrophoresis are provided, where microfluidic processing is performed on selected areas. Parts of the tape are formed by high pressure plastic film forming. Membranes and other structures are self sealing during and after penetration by pipettes and electrical probes. Rigid exoskeleton elements protect the non-rigid parts during processing and facilitate transport of the tape.

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Номер записи: 6
30-08-2012 дата публикации

Arrays of microparticles and methods of preparation thereof

Номер: US20120220495A1
Автор: Chiu Wo Chau, Hui Huang, Jiacheng Yang, Michael Seul, Sukanta Banerjee, Ye Hong
Принадлежит: Bioarray Solutions Ltd

This invention provides high unit density arrays of microparticles and methods of assembling such arrays. The microparticles in the arrays may be functionalized with chemical or biological entities specific to a given target analyte. The high unit density arrays of this invention are formed on chips which may be combined to form multichip arrays according to the methods described herein. The chips and/or multichip arrays of this invention are useful for chemical and biological assays.

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Номер записи: 7
30-08-2012 дата публикации

Methods and Microfluidic Devices for the Manipulation of Droplets in High Fidelity Polynucleotide Assembly

Номер: US20120220497A1
Автор: Joseph Jacobson, Larry Li-Yang Chu, Senthil Ramu
Принадлежит: GEN 9 Inc

Methods and devices are provided for manipulating droplets on a support using surface tension properties, moving the droplets along a predetermined path and merging two droplets together enabling a number of chemical reactions. Disclosed are methods for controlling the droplets volumes. Disclosed are methods and devices for synthesizing at least one oligonucleotide having a predefined sequence. Disclosed are methods and devices for synthesizing and/or assembling at least one polynucleotide product having a predefined sequence from a plurality of different oligonucleotides having a predefined sequence. In exemplary embodiments, the methods involve synthesis and/or amplification of different oligonucleotides immobilized on a solid support, release of synthesized/amplified oligonucleotides in solution to form droplets, recognition and removal of error-containing oligonucleotides, moving or combining two droplets to allow hybridization and/or ligation between two different oligonucleotides, and further chain extension reaction following hybridization and/or ligation to hierarchically generate desired length of polynucleotide products.

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Номер записи: 8
06-09-2012 дата публикации

Apparatus and method for separation of liquid phases of different density and for fluorous phase organic syntheses

Номер: US20120223025A1
Автор: Michal Lebl
Принадлежит: Illumina Inc

A simple, efficient apparatus and method for separating layers of immiscible or partially miscible liquids useful in methods of high-throughput combinatorial organic synthesis or parallel extraction of large libraries or megaarrays of organic compounds is disclosed. The apparatus and method are useful, whether as part of an automated, robotic or manual system for combinatorial organic synthesis or purification (extraction). In a preferred embodiment, an apparatus and method for separating layers of immiscible or partially miscible liquids compatible with microtiter plate type array(s) of reaction vessels is disclosed. Another application of centrifugation based liquid removal was found for washing the plates in biological assays or synthesis on modified substrates.

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Номер записи: 9
13-09-2012 дата публикации

Devices and methods for interfacing microfluidic devices with macrofluidic devices

Номер: US20120230887A1
Автор: Piero Zucchelli
Принадлежит: SpinX Inc

The present disclosure is directed generally to devices and methods with the purpose of interfacing microfluidic devices with macrofluidic devices. Specifically, the present disclosure includes the de-

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Номер записи: 10
13-09-2012 дата публикации

Systems and methods for high-throughput detection of an analyte in a sample

Номер: US20120231960A1
Автор: Sebastian J. Osterfeld, Shan X. Wang
Принадлежит: MagArray Inc

Provided are high-throughput detection systems. The systems include a magnetic sensor device, a magnetic field source and a reservoir plate that includes a plurality of fluid reservoirs. The magnetic sensor device includes a support with two or more elongated regions each having a magnetic sensor array disposed at a distal end. Also provided are methods in which the subject high-throughput detection systems find use.

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Номер записи: 11
27-09-2012 дата публикации

Method for Assembly of Analyte Filter Arrays Using Biomolecules

Номер: US20120245055A1
Автор: Daniel R. Mitchell, George L. Murphy, Jeremy J. John, Steve M. Savoy
Принадлежит: Nanohmics Inc

Analyte filter arrays and methods for making an analyte filter array are provided. The arrays are formed using a dispersion of filter particles having selected moieties attached to the surface of the particles and a microarray having complementary moieties formed in an array on a substrate, such that each filter particle is attached to a selected region of the microarray. The moiety on the substrate may be RNA or DNA or other molecule. The substrate may be a surface of a detector array, a membrane that may be placed in registration with the detector array or a stamp used to transfer the filter array to a detector array.

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Номер записи: 12
06-12-2012 дата публикации

Piezoelectric dispenser with a longitudinal transducer and replaceable capillary tube

Номер: US20120304929A1
Автор: Yehuda Ivri
Принадлежит: Biodot Inc

Systems, devices and methods are provided, for acquiring and dispensing predetermined volumes of liquids and, in particular, to a unique piezoelectric dispenser for acquiring and dispensing of small volumes of fluid in an automatic or manual production of DNA arrays and assays, wherein droplets are dispensed in a single drop format with volumes that may range from about a few picoliters to several nanoliters. The dispenser can advantageously utilize a disposable capillary tube assembly while desirably retaining the piezoelectric actuator for subsequent further uses, thereby mitigating the possibility of cross contamination of fluids and providing an economical and cost effective approach with reuse of the piezoelectric actuator for further operation such as with a variety of liquids to be dispensed and transferred. A unique longitudinal transducer can transmit radial tube displacement into controlled axial motion of the tube.

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Номер записи: 13
03-01-2013 дата публикации

Arrays Of Biological Membranes And Methods And Use Thereof

Номер: US20130005611A1
Автор: Anthony G. Frutos, Joydeep Lahiri, Peter J. Kalal, Steven J. Jonas, YE Fang
Принадлежит: Individual

The present invention overcomes the problems and disadvantages associated with prior art arrays by providing an array comprising a plurality of biological membrane microspots associated with a surface of a substrate that can be produced, used and stored, not in an aqueous environment, but in an environment exposed to air under ambient or controlled humidities. Preferably, the biological membrane microspots comprise a membrane bound protein. Most preferably, the membrane bound protein is a G-protein coupled receptor, an ion channel, a receptor serine/threonine kinase or a receptor tyrosine kinase.

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Номер записи: 14
28-02-2013 дата публикации

Biochip stamping device and stamping method thereof

Номер: US20130047399A1
Автор: Bo Sung Ku, Hee Ju Son, Sang Youl JEON
Принадлежит: Samsung Electro Mechanics Co Ltd

There is provided a biochip stamping device. The biochip stamping device includes a stamping jig in which a first biochip is aligned; an inverting mechanism vertically inverting a second biochip; and a movement mechanism transferring the vertically inverted second biochip on the stamping jig to combine the first biochip and the second biochip.

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Номер записи: 15
28-02-2013 дата публикации

High throughput screening of ion channels

Номер: US20130048511A1
Автор: Edward D. Verdonk
Принадлежит: Molecular Devices LLC

Multi-well plates having contoured well designs allow multi-stage high throughput parallel assaying of ion channels or ion transporters. A well of a multi-well plate has a bottom region that is sized and shaped to simultaneously accommodate a sensing electrode and a pipette for delivering test compounds, wash fluid, and optionally ligands. The multi-well plates when coupled with an instrument having a pipette head and an electrode plate facilitates fluidic contact between cells and fluids delivered via a pipette for washing of wells with buffers or other wash solutions for serial exposure of test cells to various reagents or other stimuli. The control and test experiments are performed on the same cell (or cells) in a single well.

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Номер записи: 16
30-05-2013 дата публикации

Method for fabricating a microarray of soft materials

Номер: US20130136863A1
Автор: Hiroaki Onoe, Kaori Kuribayashi, Satoshi Hiyama, Shoji Takeuchi
Принадлежит: NTT DOCOMO INC

A method for fabricating a microarray of plural soft materials includes: vapor-depositing a first layer poly(para-xylylene) resin on a substrate, forming a first micro pattern in the poly(para-xylylene) resin; obtaining a substrate including a first microarray formed by pouring a first soft material solution, freeze-drying the first soft material to obtain a micro-arrayed substrate of the freeze-dried first soft material; vapor-depositing a second layer poly(para-xylylene) resin on the micro-arrayed substrate of the freeze-dried first soft material, forming a second micro pattern placed differently from the first micro pattern by penetrating the poly(para-xylylene) resin of the first and second layers, forming a second microarray on the substrate by pouring a second soft material solution; and forming a microarray of the first and second soft materials on the substrate by peeling off the poly(para-xylylene) resin of the first and second layers.

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Номер записи: 17
13-06-2013 дата публикации

Systems and methods for high speed array printing and hybridization

Номер: US20130150266A1
Автор: Holger Eickhoff, Thomas C. Tisone
Принадлежит: Biodot Inc

Novel and improved systems and methods for high speed arraying, hybridization, quantitative development and/or assaying are provided. Some embodiments provide a web based arraying format. Some other embodiments provide a sheet based arraying format. Some embodiments use a drop on drop assaying or hybridization mode. In some embodiments, a substantially inert substrate is utilized. In some other embodiments, an interactive substrate is utilized.

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Номер записи: 18
05-12-2013 дата публикации

System for performing automated solid phase extractions

Номер: US20130323138A1
Автор: Thomas J. Demmitt
Принадлежит: Biolytic Lab Performance Inc

The invention provides an improved instrument for automation of solid phase extraction chemistries typically used in biotechnology labs. The instrument includes a mechanism for transferring samples dissolved in a liquid from initial containers to reaction columns that are used to perform solid phase extractions. Samples, reaction columns and collection containers are in microtiter plate format or tubes that are on 18 millimeter centers. The transfer system is automatically cleaned after use in preparation for the next use. A dispense manifold is used to dispense various reagents into the reaction columns. Pressure differential is used to move reagents through reaction columns. A sliding cover is used to divert the reagent exiting reaction columns to waste or allowing collection of sample as it exits outlets of reaction columns. Samples are automatically collected in microtiter plates or in individual tubes or vials.

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Номер записи: 19
26-12-2013 дата публикации

Reaction Device

Номер: US20130341322A1
Автор: Katsuyoshi Tabuse, Minoru ONCHI
Принадлежит: SUNNY ENGINEERING Co Ltd

A reaction device including a microwave oscillation means for generating microwaves; holding containers for individually holding multiple samples collected from collection targets (e.g., human targets, etc.); a microwave irradiation container on which each of the holding containers can be individually loaded; a temperature sensor for detecting the temperature of a sample held in a holding container or of the interior of the microwave irradiation container; and a microwave control means for varying the microwaves generated by the microwave oscillation means on the basis of the temperature detected by the temperature sensor. The microwave irradiation container includes: a microwave introduction port for introducing the microwaves generated by the microwave oscillation means into the microwave irradiation container; and ring-shaped patterns for irradiating each of the holding containers with the microwaves introduced from the microwave introduction port.

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Номер записи: 20
26-12-2013 дата публикации

Bead sealing method, method for detecting target molecule, array, kit, and target molecule detection device

Номер: US20130345088A1
Автор: Hiroyuki Noji, Ryota Iino, Suguru ARAKI
Принадлежит: JAPAN SCIENCE AND TECHNOLOGY AGENCY

This invention provides a technique enabling to detect target molecules of low concentration with high sensitivity. This invention includes (i) a step of introducing a hydrophilic solvent ( 42 ) containing beads ( 40 ),( 41 ′) into a space ( 30 ) between (a) a lower layer section ( 10 ) including a plurality of receptacles ( 13 ) each of which is capable of storing only one of the beads ( 41 ),( 41 ′) and which are separated from each other by a side wall ( 12 ) having a hydrophobic upper surface and (b) an upper layer section ( 20 ) facing a surface of the lower layer section ( 10 ) on which surface the plurality of receptacles ( 13 ) are provided; and (ii) a step of introducing a hydrophobic solvent ( 43 ) into the space ( 30 ), the step (ii) being carried out after the step (i).

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Номер записи: 21
06-02-2014 дата публикации

Device and method for the generation of molecular microarrays

Номер: US20140038854A1
Автор: Günter Roth, Jugen Burger
Принадлежит: Albert Ludwigs Universitaet Freiburg

The invention relates to a device and a method for the generation of molecular microarrays. The invention relates therefore to a universal approach for the generation of protein microarrays, DNA microarrays and RNA microarrays (in general nucleic acid microarrays), by production of an output molecule from a template molecule microarray via enzymatic or chemical processes and transfer of the output molecule onto the desired molecular microarray.

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Номер записи: 22
10-04-2014 дата публикации

Microvessels, microparticles, and methods of manufacturing and using the same

Номер: US20140100123A1
Автор: John A. Moon, M. Shane Bowen, Michal Lebl, Michel Perbost, Ryan C. Smith, Steven H. Modiano
Принадлежит: Illumina Inc

A plurality of isolated microvessels including a plurality of encoded microvessels each having a microbody and a reservoir core. The microbody is configured to separate a biological or chemical substance in the reservoir core from an ambient environment surrounding the microbody. The microbody includes a transparent material that at least partially surrounds the reservoir core and facilitates detection of an optical characteristic of the substance within the reservoir core. The microbody of each microvessel includes an identifiable code that distinguishes individual microvessels of the plurality of encoded microvessels from each other. The plurality of isolated microvessels also includes a plurality of compartments each configured to separate individual microvessels of the plurality of encoded microvessels from each other.

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Номер записи: 23
05-01-2017 дата публикации

SYSTEMS AND METHODS TO DISPENSE AND MIX REAGENTS

Номер: US20170001165A1
Автор: CRNOGORAC Filip, Li Bolan, McGall Glenn
Принадлежит:

The present disclosure provides methods, device, and system for wafer processing. The wafer processing apparatus uses lid dispenser to disperse at least one reagent to the surface of the wafer. Further, the wafer is positioned on top of a rotatable vacuum chuck configured to spread at least one reagent over the surface of the wafer via a centrifugal force or surface tension, thereby permitting the at least one reagent to react with an additional reagent. Further, when dispensing the at least one reagent, a separation gap between the lid dispenser and the wafer is at a predetermined distance, for example, from 50 μm to 2 mm. 1. A wafer processing apparatus comprising:a wafer conveyance robot configured to move a first wafer from a first position to a second position;a vacuum chuck at the second position, the vacuum chuck rotatably holding the first wafer;a lid dispenser at the second position, the lid dispenser aligned with the first wafer along a vertical axis;a nozzle provided by the lid dispenser and above the first wafer, the nozzle dispensing at least one reagent onto a surface of the first wafer;wherein the vacuum chuck is configured to rotate the first wafer to spread the at least one reagent over the surface of the first wafer by centrifugal force or surface tension, thereby permitting the at least one reagent to react with an additional reagent.2. The wafer processing apparatus of claim 1 , wherein when the at least one reagent is dispensed onto the surface of the first wafer claim 1 , a separation gap between the lid dispenser and the first wafer ranges from 50 μm to 2 mm.3. The wafer processing apparatus of further comprises a first wafer cassette at the first position claim 1 , the first wafer cassette being configured to hold at least one second wafer.4. The wafer processing apparatus of claim 3 , wherein the at least one second wafer includes the first wafer.5. The wafer processing apparatus of further comprises a second wafer cassette at a third ...

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Номер записи: 24
05-01-2017 дата публикации

Screening assays and methods

Номер: US20170001166A1
Автор: Hidde L. Ploegh, J. Christopher Love, Jehnna Ronan
Принадлежит: Harvard College

Screening assays and methods of performing such assays are provided. In certain examples, the assays and methods may be designed to determine whether or not two or more species can associate with each other. In some examples, the assays and methods may be used to determine if a known antigen binds to an unknown monoclonal antibody.

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Номер записи: 25
04-01-2018 дата публикации

TIP OVERLAY FOR CONTINUOUS FLOW SPOTTING APPARATUS

Номер: US20180001317A1
Автор: Eckman Joshua Wayne, Eddings Mark, Gale Bruce Kent, Miles Adam, Natarajan Sriram, Smith Jim
Принадлежит:

The present disclosure provides apparatuses, systems, and methods involving a spotter apparatus for depositing a substance from a carrier fluid onto a deposition surface in an ordered array, the spotter apparatus comprising a loading surface including a first well and a second well; and a different outlet surface, including a first opening and a second opening, where a first microconduit fluidly couples the first well with the first opening and a second microconduit fluidly couples the second well with the second opening. An overlay is sealed to the outlet surface and penetrated by a deposition channel that is situated to communicate carrier fluid among the first opening, the second opening, and the deposition surface when the overlay is pressed against the deposition surface. 1. A method of depositing at least one substance from a carrier fluid on a deposition surface , the method comprising: i) a loading surface including the first well and a second well;', 'ii) an outlet surface, different than the loading surface, including a first opening and a second opening;', 'iii) a first microconduit fluidly coupling the first well with the first opening;', 'iv) a second microconduit fluidly coupling the second well with the second opening; and', 'v) an overlay positioned on the outlet surface and penetrated by a deposition channel, wherein the deposition channel is situated to communicate carrier fluid among the first opening, the second opening, and a deposition surface when the overlay is pressed against the deposition surface, and, 'a) loading a carrier fluid having at least one substance into a first well of a spotter apparatus, wherein the spotter apparatus comprisesb) flowing the carrier fluid in series from the first well, through the first microconduit to the deposition channel, through the second microconduit and into the second well, wherein the deposition channel directs at least one substance from the carrier fluid onto the deposition surface.2. The method of ...

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Номер записи: 26
04-01-2018 дата публикации

NANOPIPETTE APPARATUS FOR MANIPULATING CELLS

Номер: US20180002170A1
Автор: Actis Paolo, Pourmand Nader, Seger R. Adam, Vilozny Boaz
Принадлежит:

Disclosed herein are methods and systems for controlled ejection of desired material onto surfaces including in single cells using nanopipettes, as well as ejection onto and into cells. Some embodiments are directed to a method and system comprising nanopipettes combined with an xyz controller for depositing a user defined pattern on an arbitrary substrate for the purpose of controlled cell adhesion and growth. Alternate embodiments are directed to a method and system comprising nanopipettes combined with an xyz controller and electronic control of a voltage differential in a bore of the nanopipette electroosmotically injecting material into a cell in a high-throughput manner and with minimal damage to the cell. Yet other embodiments are directed to method and system comprising functionalized nanopipettes combined with scanning ion conductance microscopy for studying molecular interactions and detection of biomolecules inside a single living cell. 132.-. (canceled)33. A method of detecting an analyte in a single living cell , comprising: an amplifier for nanopipette bias and current measurement,', 'a micromanipulator for control in the X, Y and Z directions,', 'a piezo-actuator for fine control in the X, Y and Z directions, and', 'a configurable integrated circuit;, 'inserting the tip of a nanopipette to a defined depth into a single living cell, wherein the nanopipette is functionalized with an analyte-binding reagent and is comprised in a system further comprisingmonitoring current through the tip of the nanopipette;detecting an analyte in the single living cell by detecting a reduction in current through the tip of the nanopipette.34. The method according to claim 33 , wherein the inserting is at a speed of from 50 to 200 μm/s.35. The method according to claim 34 , wherein the inserting is at a speed of 80 μm/s or greater.36. The method according to claim 33 , wherein the analyte-binding reagent is immobilized on an interior surface of the nanopipette at or near ...

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Номер записи: 27
04-01-2018 дата публикации

IN SITU HEAT INDUCED ANTIGEN RECOVERY AND STAINING APPARATUS AND METHOD

Номер: US20180003601A1
Автор: Angros Lee H.
Принадлежит:

An automated microscope slide staining system and staining apparatus and method that features a plurality of individually operable miniaturized pressurizable reaction compartments or a pressurizable common chamber for individually and independently processing a plurality of microscope slides. The apparatus preferably features independently movable slide support elements each having an individually operable heating element. 1. A microscope slide staining system , comprising:a chamber having an inner space;a plurality of independently movable slide support elements positioned in the inner space of the chamber, each of the slide support elements configured to support a microscope slide;a spreading device supply station containing a plurality of spreading devices, each of the spreading devices being positionable in association with at least one of the microscope slides supported on the slide support elements in a way that the spreading device defines a gap between the spreading device and the microscope slide when the microscope slide is positioned on the slide support element and in a way that each of the spreading devices is independently movable relative to the other spreading devices to spread at least one reagent on at least a portion of the microscope slide;at least one positioning device operable in a way to pick up and carry at least one of the spreading devices from the spreading device supply station to one of the slide support elements so that the at least one spreading device is associated with the microscope slide supported on the slide support element; anda plurality of heating elements such that at least one of the heating elements is associated with one of the slide support elements to heat the at least one reagent on the microscope slide.2. The microscope slide staining system of claim 1 , wherein each of the spreading devices engages side edges of the microscope slide when the microscope slide is positioned on the slide support element.3. The ...

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Номер записи: 28
12-01-2017 дата публикации

Method of Distributing Discrete Polymer Networks

Номер: US20170007977A1
Автор: Alexander MASTROIANNI, John Scott, Scott C. Benson, Steven M. Menchen
Принадлежит: Life Technologies Corp

A method of preparing a discrete polymer network array include mixing a plurality of nucleic acid polymer networks with a plurality of color-activated polymer networks to form a dispersion, applying the dispersion to an array of wells, the nucleic acid polymer networks selectively depositing into wells of the array of wells, and rinsing the array of wells to selectively remove the plurality of color-activated polymer networks.

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Номер записи: 29
14-01-2016 дата публикации

Synthesis apparatus and method

Номер: US20160008815A1
Автор: Neil Porter, Paul James ROTHWELL
Принадлежит: Touchlight Genetics Ltd

Apparatus for biochemical synthesis comprises a reaction vessel, a temperature control device for the reaction vessel, a plurality of reservoirs for reaction components, and a supply/withdrawal means, e.g. comprising reciprocating syringe pumps and switchable valves. Agitation of a reaction mixture is effected by withdrawing a part of the mixture from the reaction vessel and returning it thereto.

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Номер записи: 30
27-01-2022 дата публикации

FORMATION OF ARRAY OF MEMBRANES AND APPARATUS THEREFOR

Номер: US20220023819A1
Автор: Bahamon Pedro Miguel Ortiz, Brown Clive Gavin, Hyde Jason Robert, John Andrew, Mackett Paul Raymond
Принадлежит: Oxford Nanopore Technologies Ltd.

An array of membranes comprising amphipathic molecules is formed using an apparatus comprising a support defining an array of compartments. Volumes comprising polar medium are provided within respective compartments and a layer comprising apolar medium is provided extending across the openings with the volumes. Polar medium is flowed across the support to displace apolar medium and form a layer in contact with the volumes, forming membranes comprising amphipathic molecules at the interfaces. In one construction of the apparatus, the support that comprises partitions which comprise inner portions and outer portions. The inner portions define inner recesses without gaps therebetween that are capable of constraining the volumes comprising polar medium contained in neighbouring inner recesses from contacting each other. The outer portions extend outwardly from the inner portions and have gaps allowing the flow of an apolar medium across the substrate. 162-. (canceled)63. An apparatus for forming an amphiphilic membrane , the apparatus comprising:a substrate comprising a recess, wherein the recess is configured to contain polar medium, and wherein the recess opens at a surface of the substrate; andpillars, wherein the pillars extend from the surface of the substrate and extend parallel to the axis normal to the surface of the substrate, and wherein gaps between the pillars are configured to allow flow of an apolar medium;wherein the pillars are configured to support an amphiphilic membrane arranged to contact polar medium contained in a respective recess.64. The apparatus of claim 63 , further comprising a volume of polar medium contained in the recess.65. The apparatus of claim 64 , further comprising a layer comprising apolar medium extending across the substrate in contact with the volume of polar medium.66. The apparatus of claim 64 , further comprising: a layer comprising polar medium extending across the substrate claim 64 , wherein the layer comprising polar ...

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Номер записи: 31
10-01-2019 дата публикации

Apparatus Enabling High Density Information Storage in Molecular Chains

Номер: US20190009240A1
Автор: Frank Ian Ward, Korn Jeffrey A., Magyar Andrew P., Patel Neil Sunil
Принадлежит:

A parallelized chain-synthesizing technique includes an array of wells, each well in the array providing a location where a specific arbitrary sequence for polymeric chains can be grown. An optical addressing system selectively delivers light to the wells to mediate or control reactions in the wells. 1. A parallelized chain-synthesizing apparatus , comprising:an array of locations for growing different arbitrary sequences for polymeric chains; andan optical addressing system for selective delivery of light to the locations to mediate or control chemical reactions for the growth of the different sequences at the locations.2. The parallelized chain-synthesis apparatus of claim 1 , wherein the polymeric chains are DNA strands.3. A microfluidic device comprising the chain-synthesis apparatus of .4. A parallelized chain-synthesizing apparatus claim 1 , comprising an array of wells claim 1 , wherein each well provides a location for growing a specific arbitrary sequence for polymeric chains and each well is provided with an optical filter or shutter claim 1 , each optical filter or shutter being configured to independently allow or block radiation from a light source.5. The parallelized chain-synthesizing apparatus of claim 4 , wherein the optical filter or shutter is provided in a filter array.6. The parallelized chain-synthesizing apparatus of claim 5 , wherein light from the light source is polarized and the filter array is a pixilated liquid crystal array.7. A parallelized chain-synthesizing apparatus claim 5 , comprising an array of wells claim 5 , each well providing a location for growing a specific arbitrary sequence for polymeric chains claim 5 , and an array of light emitting diodes claim 5 , wherein a diode in the array of diodes is coupled with a well in the array of wells.8. A parallelized chain-synthesizing apparatus claim 5 , comprising an array of wells claim 5 , each well providing a location for growing a specific arbitrary sequence for polymeric chains ...

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Номер записи: 32
14-01-2021 дата публикации

KINETIC EXCLUSION AMPLIFICATION OF NUCLEIC ACID LIBRARIES

Номер: US20210010071A1
Автор: Bevis-Mott Claire, Boutell Jonathan, Hunter Shaun, McInerney Peter
Принадлежит:

An example method includes reacting a first solution and a different, second solution on a flow cell by flowing the first solution over amplification sites on the flow cell and subsequently flowing the second solution over the amplification sites. The first solution includes target nucleic acids and a first reagent mixture that comprises nucleoside triphosphates and replication enzymes. The target nucleic acids in the first solution transport to and bind to the amplification sites at a transport rate. The first reagent mixture amplifies the target nucleic acids that are bound to the amplification sites to produce clonal populations of amplicons originating from corresponding target nucleic acids. The amplicons are produced at an amplification rate that exceeds the transport rate. The second solution includes a second reagent mixture and lacks the target nucleic acids. The second solution is to increase a number of the amplicons at the amplification sites. 1. A fluidic system , comprising:a reagent manifold including at least one valve in fluid communication with an inlet port of a flow cell that includes an array of amplification sites, the reagent manifold further including a plurality of channels fluidly connected between the at least one valve and corresponding reagent reservoirs; anda controller including one or more processors, the controller to control the at least one valve and a pump to flow a first solution through the inlet port over the array of amplification sites on the flow cell and to subsequently flow a different, second solution through the inlet port over the array of amplification sites on the flow cell;wherein the first solution includes a number of target nucleic acids and a first reagent mixture that comprises nucleoside triphosphates (NTPs) and one or more replication enzymes, the number of target nucleic acids in the first solution exceeding a number of the amplification sites in the array, the first solution reacting on the flow cell to ...

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Номер записи: 33
03-02-2022 дата публикации

SCREENING ASSAYS AND METHODS

Номер: US20220032257A1
Автор: Love J. Christopher, Ploegh Hidde L., Ronan Jehnna
Принадлежит:

Screening assays and methods of performing such assays are provided. In certain examples, the assays and methods may be designed to determine whether or not two or more species can associate with each other. In some examples, the assays and methods may be used to determine if a known antigen binds to an unknown monoclonal antibody. 1. A printed microarray of unknown cell-derived products , each position of said array comprising a deposit corresponding to a composition of a single cell , said deposit being less than 100 micrometers in diameter.2. The microarray of claim 1 , wherein said deposit is a secreted cell-derived product selected from the group consisting of an antibody claim 1 , cytokine claim 1 , chemokine claim 1 , and inflammatory mediator.3. The microarray of claim 1 , wherein said microarray comprises a capture ligand for a class of secreted products.4. The microarray of claim 3 , wherein said capture ligand binds to a single immunoglobulin isotype. This application claims priority to U.S. Ser. No. 60/717,976 filed Sep. 16, 2005, which is incorporated herein by reference in its entirety.This invention was funded in part by the U.S. Government under grant numbers NAKFI Nano08 awarded by the National Academy of Sciences and/or grant 5R01AI034893-1 awarded by the National Institutes of Health. The Government has certain rights in the invention.The invention relates to screening assays and methods to identify secreted products.Assays exist to identify compounds or molecules of interest that may be involved in a disease process or other condition or in treating a disease process or condition. Existing assays have some drawbacks. One significant drawback is the time required to screen for many compounds or molecules. Another drawback is that it may not be possible to recover the compound or molecule post-screening. There remains a need for better screening assays and methods.The invention provides a printed microarray of unknown cell-derived products. Each ...

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Номер записи: 34
03-02-2022 дата публикации

APPARATUS, SYSTEM, AND METHOD USING IMMISCIBLE-FLUID-DISCRETE-VOLUMES

Номер: US20220033896A1
Автор: Cox David M., Lee Linda G., Ma Congcong, Oldham Mark F., REEL Richard T., SCHROEDER Benjamin G., SORENSON Jon M., WIYATNO Willy, WOO Sam L.
Принадлежит: APPLIED BIOSYSTEMS, LLC

Various embodiments of the teachings relate to a system or method for sample preparation or analysis in biochemical or molecular biology procedures. The sample preparation can involve small volume processed in discrete portions or segments or slugs, herein referred to as discrete volumes. A molecular biology procedure can be nucleic acid analysis. Nucleic acid analysis can be an integrated DNA amplification/DNA sequencing procedure. 1. A method comprising:contacting a stream of aqueous sample fluid flowing in a first conduit with a stream of non-aqueous spacing fluid that is immiscible with the aqueous sample fluid to form discrete volumes of the aqueous sample fluid separated from one another by the non-aqueous spacing fluid, wherein the aqueous sample fluid comprises target nucleic acid, and wherein a first plurality of the discrete volumes contains at least one molecule comprising the target nucleic acid and a second plurality of the discrete volumes contains no molecules comprising the target nucleic acid;amplifying the target nucleic acid in one or more of the first plurality of the discrete volumes to form an amplicon;in a second conduit, detecting a fluorescence signal from the amplicon in the one or more of the first plurality of the discrete volumes; andbased on the detecting, discriminating between the one or more of the first plurality of the discrete volumes and the second plurality of the discrete volumes.2. The method of claim 1 , wherein the contacting comprises continuously flowing at least one of the aqueous sample fluid and the non-aqueous spacing fluid into the first conduit.3. The method of claim 1 , further comprising separating the second plurality of the discrete volumes from the first plurality of the discrete volumes.4. The method of claim 1 , wherein less than 37% of the first plurality of the discrete volumes comprise a single molecule comprising the target nucleic acid.5. The method of claim 4 , wherein 1% or more of the first plurality ...

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Номер записи: 35
18-01-2018 дата публикации

PATTERNING DEVICE

Номер: US20180015437A1
Автор: Lammertyn Jeroen, WITTERS Daan
Принадлежит: KATHOLIEKE UNIVERSITEIT LEUVEN, K.U.LEUVEN R&D

A novel miniaturized and highly automated method for the controlled printing of large arrays of nano- to femtoliter droplets is presented by actively transporting mother droplets over hydrophilic-in-hydrophobic micropatches. The proposed technology consists of single plate or double-plate devices where mother droplets can be actuated and hydrophilic-in-hydrophobic micropatches on one or both plates of the device where nano- to femtoliter droplets are printed. Due to the selective wettability of the more wettable hydrophilic micropatches in a hydrophobic matrix, large nano- to femtoliter droplet arrays are created when mother droplets are transported over these arrays. The parent droplets can be moved by different droplet actuation principles, for example, by using the principle of electrowetting-on-dielectric droplet actuation. We propose another method that uses two plates that are placed on top of each other while being separated by a spacer. One plate is dedicated to confirming and guiding of parent droplets by using hydrophilic patches in a hydrophobic matrix, while the other plate contains hydrophilic-in-hydrophobic arrays dedicated to the printing of nano- to femtoliter droplets. When the plate dedicated to parent droplet guiding is rotated over the plate dedicated to printing of nano- to femtoliter droplets, nano- to femtoliter droplets are dispensed inside the hydrophilic-in-hydrophobic array due to their selective wettability. All these proposed methods allow the parent droplets to be moved over the hydrophilic-in-hydrophobic arrays many times, providing unique advantages for performing bio-assays or miniaturized materials synthesis in nano- to femtoliter sized droplets. Upon the controlled evaporation of the dispensed droplets of solution, large arrays of the printed material can be generated on an automated way in seconds of time on a very flexible way. The method disclosed herein provides a distinct nano- to femtoliter droplet printing technique for a ...

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Номер записи: 36
21-01-2016 дата публикации

Integrated Analysis System

Номер: US20160016140A1
Автор: Jovanovich Stevan B., Stern Seth, Van Gelder Ezra, Vangbo Mattias
Принадлежит: INTEGENX INC.

The invention provides systems, devices, methods, and kits for performing an integrated analysis. The integrated analysis can include sample processing, library construction, amplification, and sequencing. The integrated analysis can be performed within one or more modules that are fluidically connected to each other. The one or more modules can be controlled and/or automated by a computer. The integrated analysis can be performed on a tissue sample, a clinical sample, or an environmental sample. The integrated analysis system can have a compact format and return results within a designated period of time.

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Номер записи: 37
15-01-2015 дата публикации

Method for detecting the presence of a target nucleic acid sequence in a sample

Номер: US20150017709A1
Автор: James F. Brown, Jonathan E. Silver
Принадлежит: APPLIED BIOSYSTEMS LLC, US Department of Health and Human Services

A method comprises loading a sample portion into a sample chamber which comprises means for minimizing diffusion of the sample portion, subjecting the sample portion to an amplification step, and determining whether the sample portion contains at least one molecule of a target nucleic acid. If the sample portion contains a single molecule of the target nucleic acid, the sample portion would attain a detectable concentration of the target nucleic acid after a single round of amplification. Also, a microfluidic device comprising a sample portion and a sample chamber comprising means for minimizing diffusion of the sample portion. Also, a microfluidic device comprising a sample chamber and an amplification targeting reagent positioned in the first sample chamber.

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Номер записи: 38
19-01-2017 дата публикации

MICROARRAY BASED SAMPLE DETECTION SYSTEM

Номер: US20170016052A1
Автор: COONEY Christopher G., PARKER Jennifer, PEROV Alexander, Qu Peter Qiang
Принадлежит:

A microarray assembly for detection of a target molecule is disclosed. The microarray assemblies comprise an array chamber having a microarray located therein and features that facilitate liquid movement within the array chamber. Also disclosed are methods for making the microarray assembly using rollable films and methods for detecting microarray spots using an internal control fluorophore in the array spot. 1. A microarray assembly for detection of a target molecule in a sample , comprising: an array chamber with a sample inlet at a first end , a sample outlet at a second end , a top interior surface , a bottom interior surface , side walls and a microarray located on the bottom interior surface; anda waste chamber that is in fluid communication with the outlet of the array chamber,wherein the array chamber comprises a hydrophilic interior surface positioned to facilitate complete filling of the array chamber by a water-based fluid and the continuous flow of the fluid from the sample inlet to the sample outlet and wherein the cross-sectional area at the first end of the array chamber is larger than the cross-sectional area at the second end of the array chamber.2. The microarray assembly of claim 1 , wherein the array chamber is in the shape of a tubular channel and wherein the cross-sectional area of the array chamber decreases in a continuous manner from the first end to the second end.3. The microarray assembly of claim 1 , wherein the cross-sectional area of the array chamber decreases in a stepwise manner from the first end to the second end.4. The microarray assembly of claim 1 , wherein the cross-sectional area of the array chamber at the first end is three-times larger than the cross-sectional area of the array chamber at the second.5. The microarray assembly of claim 1 , wherein the microarray comprises a plurality of array spots arranged in a single row extending from the first end to the second end of the array chamber.6. The microarray assembly of ...

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Номер записи: 39
18-01-2018 дата публикации

Systems and Methods for Multiple Analyte Detection

Номер: US20180016624A1
Автор: Beard Nigel P., Chiang Yuh-Min, Dromaretsky Alexander, Ermakov Sergey V., Harding Ian A., LEHTO Dennis, Liu David M., Oldham Mark F., Roy Joy, Shariati Maryam, Ulmanella Umberto, Van Camp John R., Vann Charles S., Yang Joon Mo, Yue Min
Принадлежит:

Systems and methods for multiple analyte detection include a system for distribution of a biological sample that includes a substrate, wherein the substrate includes a plurality of sample chambers, a sample introduction channel for each sample chamber, and a venting channel for each sample chamber. The system may further include a preloaded reagent contained in each sample chamber and configured for nucleic acid analysis of a biological sample that enters the substrate and a sealing instrument configured to be placed in contact with the substrate to seal each sample chamber so as to substantially prevent sample contained in each sample chamber from flowing out of each sample chamber. The substrate can be constructed of detection-compatible and assay-compatible materials. 1. A system for distribution of a biological sample , the system comprising:a substrate, wherein the substrate comprises a plurality of sample chambers, a sample introduction channel for each sample chamber, and a venting channel for each sample chamber;a preloaded reagent contained in each sample chamber and configured for nucleic acid analysis of a biological sample that enters the substrate; anda sealing instrument configured to be placed in contact with the substrate to seal each sample chamber so as to substantially prevent sample contained in each sample chamber from flowing out of each sample chamber,wherein the substrate is constructed of detection-compatible and assay-compatible materials.2. The system of claim 1 , wherein the sealing instrument is configured to be placed in contact with an exterior portion of the substrate.3. (canceled)4. (canceled)5. The system of claim 1 , wherein the sealing instrument is configured to be placed in contact with the substrate at least one of before a reaction process that occurs in the sample chambers and during a reaction process that occurs in the sample chambers.6. The system of claim 5 , wherein the sealing plate is configured to be placed in contact ...

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Номер записи: 40
22-01-2015 дата публикации

Method and Apparatus for the Analysis and Identification of Molecules

Номер: US20150021183A1
Автор: Daniel Wai-Cheong So
Принадлежит: Individual

An apparatus and method for performing analysis and identification of molecules have been presented. In one embodiment, a portable molecule analyzer includes a sample input/output connection to receive a sample, a nanopore-based sequencing chip to perform analysis on the sample substantially in real-time, and an output interface to output result of the analysis.

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Номер записи: 41
10-02-2022 дата публикации

APPARATUS AND METHOD FOR ANALYZING REACTIONS

Номер: US20220040661A1
Автор: LANGE DE OLIVEIRA Armin
Принадлежит:

The invention proceeds from an apparatus for analyzing reactions, comprising a starting material distributor () and at least two reactors () which are connected in parallel and are each connected via a connecting conduit () to an outlet of the starting material distributor (). To set the inflow, a pressure regulator () and a restrictor () are installed in each connecting conduit () between the starting material distributor () and the reactors () or an outlet conduit () in which a restrictor () and a pressure regulator () are installed branches off from each connecting conduit (). 1735733195731319335. An apparatus for analyzing reactions , comprising a starting material distributor () and at least two reactors () which are connected in parallel and are each connected via a connecting conduit () to an outlet of the starting material distributor () , wherein a pressure regulator () and a restrictor () are installed in each connecting conduit () between the starting material distributor () and the reactors () in order to set the inflow or an outlet conduit () in which a restrictor () and a pressure regulator () are installed branches off from each connecting conduit ().233719. The apparatus according to claim 1 , wherein the pressure regulator () is an exit pressure regulator and is installed between the starting material distributor () and the restrictor ().333193. The apparatus according to claim 1 , wherein the pressure regulator () is an admission pressure regulator and is installed between the restrictor () and the reactor ().419. The apparatus according to claim 1 , wherein the restrictor () is a capillary claim 1 , a microstructured component claim 1 , an orifice plate or a nozzle.539. The apparatus according to claim 1 , wherein the reactors () are connected to a further distributor ().61993. The apparatus according to claim 1 , wherein a restrictor () is positioned between the further distributor () and each reactor ().7339191993. The apparatus according to ...

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Номер записи: 42
22-01-2015 дата публикации

Two-Step System And Method For The Production Of Methyl Isobutyl Ketone

Номер: US20150025277A1
Автор: "OYoung Drow Lionel", Chan Hok Chung, Kelkar Madhura, Kelkar Vaibhav V., Wibowo Christianto
Принадлежит: ClearWaterBay Technology, Inc.

Embodiments of the present invention describe systems and methods for production of methyl isobutyl ketone (MIBK) from acetone and hydrogen in a two-step process. In a first step, acetone is converted to a product stream containing mesityl oxide (MO) at a temperature in the range of about 0-120° C. and a pressure in the range of about 1-3 atm. The composition of the product stream from the first reaction step is adjusted so that the resulting stream can undergo a favorable liquid-liquid separation in a decanter, and an MO rich product stream can be recovered. The composition of the feed to the decanter is controlled by choosing the number of reactor stages for the first reaction step and their operating temperatures, and/or by recycling some MIBK to the decanter feed. The method does not require a substantially complete conversion of acetone in the first reaction step, nor does it require a removal of DAA from the product of the first reaction step by separation. 1. A method for producing methyl isobutyl ketone (MIBK) from acetone and hydrogen , comprising:in a first reaction step, introducing acetone, where at least a portion of the acetone undergoes a condensation reaction to form diacetone alcohol (DAA), followed by a dehydration reaction that converts at least a portion of the DAA to mesityl oxide (MO), in the presence of a suitable catalyst;controlling the composition of the outlet of the first reaction step such that a favorable liquid-liquid phase split occurs, producing two liquid phases that can be subsequently separated in a decanter;in a second reaction step, contacting the organic phase containing MO with hydrogen, where MO is converted to MIBK in the presence of a suitable hydrogenation catalyst; andpurifying the MIBK-rich stream from the second reaction step to produce high purity MIBK.2. The method according to claim 1 , where the condensation and dehydration reaction occurs at a temperature in the range of about 20-120° C. and a pressure in the range ...

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Номер записи: 43
24-01-2019 дата публикации

ARRAYS OF MICROPARTICLES AND METHODS OF PREPARATION THEREOF

Номер: US20190024156A1
Автор: BANERJEE Sukanta, Chau Chiu Wo, Hong Ye, Huang Hui, Seul Michael, Yang Jiacheng
Принадлежит:

This invention provides high unit density arrays of microparticles and methods of assembling such arrays. The microparticles in the arrays may be functionalized with chemical or biological entities specific to a given target analyte. The high unit density arrays of this invention are formed on chips which may be combined to form multichip arrays according to the methods described herein. The chips and/or multichip arrays of this invention are useful for chemical and biological assays. 1. A system for performing bioassays , said system comprising at least one biochip , wherein at least one biochip comprises a patterned chip and a plurality of bio-functionalized , optically encoded beads affixed thereto.2. A method for producing biochips comprising patterning a substrate to form a plurality of chip regions , partially scribing between the chip regions , assembling at least one bead array comprising bio-functionalized , optically encoded beads on a surface of the wafer in at least one chip region; and singulating the wafer to form individual biochips.3. A method of performing bioassays comprising contacting a plurality of biochips bonded to a carrier with a solution comprising at least one target analyte , and detecting the target analyte.4. The method according to claim 3 , wherein the plurality of biochips comprises at least two subpopulations of biochips with differently biofunctionalized arrays.5. The method according to claim 3 , wherein the plurality of biochips comprises at least two subpopulations of biochips claim 3 , wherein the biochips of the different sub-populations are different sizes.6. The method according to claim 3 , wherein the plurality of biochips comprises at least two subpopulations of biochips with different bead array geometries.7. The method according to claim 3 , wherein the plurality of biochips comprises at least two subpopulations with at least two characteristics taken from the group of characteristics consisting of differently ...

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Номер записи: 44
28-01-2021 дата публикации

PARALLEL ORGANIC SYNTHESIS ON PATTERNED PAPER USING A SOLVENT-REPELLING MATERIAL

Номер: US20210023523A1
Автор: DEISS Frederique, Derda Ratmir
Принадлежит:

The present application is directed to a porous support for parallel organic synthesis comprising: a solvophilic area for spotting an organic solvent comprising a reagent for synthesizing an organic compound. and a solvophobic area that repels the organic solvent. Methods of synthesizing the support and compounds thereon are also provided. 1. A method of fabricating a porous support for performing organic synthesis or biochemical assays in an organic solvent. comprising the steps of:(a) applying a pattern of a hydrophobic material onto the porous support, the pattern defining unmodified areas separated from modified area;(b) protecting the unmodified areas with an aqueous solution;(c) applying a solvophobic material to the modified area;(d) removing the protective aqueous solution to yield a porous support with unmodified areas separated from modified solvophobic area.2. The method of wherein the protective aqueous solution creates a convex droplet covering an unmodifed area.3. The method of claim 1 , wherein the protective aqueous solution is a sucrose solution.4. The method of wherein the solvophobic material is a perfluorinated polymer.5. The method of wherein the hydrophobic material pattern is performed by wax printing.6. The method of wherein the protective aqueous material is an aqueous gel.7. The method of wherein the aqueous gel is agarose.8. The method of wherein the solvophobic material is applied in a solvent. which is removed by evaporation under conditions which prevent evaporation of water.9. The method of wherein the protective aqueous solution is removed by rinsing with water.10. The method of wherein at least one of the patterning step claim 1 , protection step and the solvphobic material application step is automated with a robotic spotter.11. A method of synthesizing one or more compounds on a porous support produced by the method of claim 1 , comprising;(a) applying one or more solvents comprising one or more reagents in an unmodified. area, ...

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Номер записи: 45
24-01-2019 дата публикации

DEVICE FOR POWDER METERING FOR CHEMICAL PRODUCTION PROCESSES UNDER CLEAN-ROOM CONDITIONS, USE THEREOF AND METERED ADDITION METHOD

Номер: US20190025107A1
Автор: Popova Irene, Rueckl Christian, Stahl Juerg
Принадлежит:

Device for metering powder, in particular in clean-rooms, which includes a vessel containing powder and a sealing head with a septum for the vessel, wherein the sealing head is connectable powder-tight with the vessel and the septum powder-tight with the sealing head and the device further includes a vessel holder, which serves to hold the sealing head of the vessel, and the vessel with its opening points downwards, so that the powder can flow out of the vessel, wherein a gap is provided between the sealing head and a holding bowl of the vessel holder, in which a gas flow between the holding bowl and the sealing head can be created. The invention also relates to a use of the device and a metered addition method. 1. A device for metering powder , which comprises a vessel containing powder and a sealing head with a septum for the vessel , wherein the sealing head is connectable powder-tight with the vessel and the septum with the sealing head , and the device further comprises a vessel holder , which serves to hold the sealing head of the vessel , and the vessel with its opening points downwards , so that the powder can flow out of the vessel , wherein a gap is present between the sealing head and a holding bowl of the vessel holder , in which a gas flow between the holding bowl and the sealing head can be created.2. The device according to claim 1 , wherein the cross-section of the gap between the holding bowl and the sealing head decreases in the direction of the septum claim 1 , so that the flow rate of the gas increases in the direction of the septum.3. The device according to one of claim 1 , wherein the gap between the holding bowl and the sealing head is shaped such that the flow rate is maximal on the septum and the flow is guided against the septum in order to remove powder particles from the septum and the sealing head.4. The device according to claim 1 , wherein the sealing head has a septum adapter and a septum cap with an opening claim 1 , wherein the ...

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Номер записи: 46
02-02-2017 дата публикации

Systems for Filling a Sample Array by Droplet Dragging

Номер: US20170028376A9
Автор: Brenan Colin J.H., Hunter Ian, Kanigan Tanya S.
Принадлежит: Massachusetts Institute of Technology

A method and an array filling system for loading a plurality of disparate sample containers, the sample containers comprising an integral structure. Each receptacle is characterized by a hydrophilic surface,, and the receptacles are separated by a hydrophobic surface. The system has a liquid transfer device capable of holding liquid and adapted for motion to cause sequential communication of liquid held in the liquid transfer device with successive receptacles of the array by dragging the liquid across the hydrophobic surface. 18.-. (canceled)9. A system for performing a biological assay , comprisinga first spool configured to unwind a sheet comprising a plurality of containers for holding a biological solution;a second spool configured to wind the sheet;a dispenser configured to load the containers;wherein the dispenser loads the containers when the sheet is unwound from the first spool.10. The system of claim 9 , further comprising a sheet comprising a plurality of containers for holding a biological solution.11. The system of claim 10 , wherein the sheet comprises a plurality of registration holes.12. The system of claim 10 , further comprising a mixing area for mixing liquid into at least some of the containers.13. The system of claim 9 , further comprising an optical detector configured to detect at least one of a fluorescence or a chemi-luminescence of a biological solution contained within at least some of the containers.14. The system of claim 9 , wherein the plurality of containers comprises an array of through-holes.15. The system of claim 14 , wherein sheet comprises a hydrophobic exterior and hydrophilic through-holes.16. A method of performing a biological assay claim 14 , comprisingproviding a plurality of containers for holding a biological solution;unwinding the sheet from a first spool;winding the sheet onto a second spool;loading a biological solution into the containers from a dispenser as the sheet is unwound from the first spool.17. The method ...

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Номер записи: 47
01-02-2018 дата публикации

DE NOVO SYNTHESIZED GENE LIBRARIES

Номер: US20180029001A1
Автор: Banyai William, Chen Siyuan, FERNANDEZ Andres, Indermuhle Pierre, Peck Bill James
Принадлежит:

De novo synthesized large libraries of nucleic acids are provided herein with low error rates. Further, devices for the manufacturing of high-quality building blocks, such as oligonucleotides, are described herein. Longer nucleic acids can be synthesized in parallel using microfluidic assemblies. Further, methods herein allow for the fast construction of large libraries of long, high-quality genes. Devices for the manufacturing of large libraries of long and high-quality nucleic acids are further described herein. 1. A polynucleotide cDNA library based on instructions provided in a computer readable non-transient medium , the library comprising at least 20 ,000 polynucleotides based on the instructions provided in the computer readable non-transient medium , wherein the at least 20 ,000 polynucleotides collectively encode cDNA sequences for at least 500 genes or gene fragments , and wherein the at least 20 ,000 polynucleotides encode sequences with an aggregate error rate of less than 1 in 800 bases compared to sequences received in the instructions provided in the computer readable non-transient medium.2. The polynucleotide cDNA library of claim 1 , wherein the at least 20 claim 1 ,000 polynucleotides encode sequences with an aggregate error rate of less than 1 in 1000 bases compared to sequences received in the instructions provided in the computer readable non-transient medium.3. The polynucleotide cDNA library of claim 1 , wherein each of the at least 20 claim 1 ,000 polynucleotides comprises a first overlap region which is complementary to a second overlap region of another polynucleotide of the at least 20 claim 1 ,000 polynucleotides.4. The polynucleotide cDNA library of claim 3 , wherein the first overlap region comprises a GC content of 35% to 65%.5. The polynucleotide cDNA library of claim 3 , wherein the first overlap region comprises 10 to 100 bases in length.6. The polynucleotide cDNA library of claim 3 , wherein the at least 20 claim 3 ,000 ...

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Номер записи: 48
01-02-2018 дата публикации

HIGH-PRESSURE POLYMERIZATION PROCESS OF ETHYLENICALLY UNSATURATED MONOMERS

Номер: US20180030160A1
Автор: Backes Udo, Cabuk Hicran, Finette Andre-Armand, Gonioukh Andrei, Wolfram Sven
Принадлежит: BASELL POLYOLEFINE GMBH

A process for polymerizing or copolymerizing ethylenically unsaturated monomers in the presence of free-radical polymerization initiators, wherein the polymerization is carried out at temperatures from 100° C. to 350° C. and pressures in the range of from 110 MPa to 500 MPa in a continuously operated polymerization reactor which is controlled by a pressure control valve at the outlet of the polymerization reactor, the process comprising continuously monitoring the pressure within the polymerization reactor, feeding a pressure signal to a controller for controlling the control valve and having the controller altering the opening of the pressure control valve to control the pressure within the polymerization reactor, wherein the controller starts an emergency shutdown program when the pressure control valve closes more than a preset threshold value and the pressure within the polymerization reactor decreases below a preset pressure threshold. 1. A process for polymerizing or copolymerizing ethylenically unsaturated monomers in the presence of free-radical polymerization initiators , wherein the polymerization is carried out at temperatures from 100° C. to 350° C. and pressures in a range of from 110 MPa to 500 MPa in a continuously operated polymerization reactor which is controlled by a pressure control valve at the outlet of the polymerization reactor and the monomer mixture is brought to the polymerization pressure by a combination of a primary compressor and a secondary compressor ,the process comprising continuously monitoring the pressure within the polymerization reactor by one or more pressure sensors creating a pressure signal indicative of the pressure within the polymerization reactor, feeding the pressure signal to a controller for controlling the control valve and having the controller altering the opening of the pressure control valve to control the pressure within the polymerization reactor,wherein the controller starts an emergency shutdown program ...

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Номер записи: 49
17-02-2022 дата публикации

DROPLET LIBRARIES

Номер: US20220047998A1
Автор: Hutchison John Brian, Link Darren Roy, Samuels Michael L., Weiner Michael
Принадлежит:

The present invention generally relates to droplet libraries and to systems and methods for the formation of libraries of droplets. The present invention also relates to methods utilizing these droplet libraries in various biological, chemical, or diagnostic assays. 114.-. (canceled)15. A method for detecting target molecules in a fluid sample , the method comprising:providing, to a microfluidic device, a fluid sample comprising target molecules, a plurality of beads each comprising a reactive component for binding a target molecule thereto, and a detection component for binding to a target molecule that is bound to a bead;partitioning, in the microfluidic device, the fluid sample into plurality of separate and isolated partitions of fluid, wherein at least one of the plurality of partitions comprises an immunocomplex comprising a target molecule bound between a single bead, via an associated reactive component, and an associated detection component; andmonitoring each of the plurality of partitions for detection of an event associated with contents of one or more of the plurality of partitions.16. The method of claim 15 , wherein the plurality of partitions comprises a first subset and a second subset claim 15 , wherein the first subset comprises partitions that each comprise no bead and the second subset comprises partitions that each comprise a single bead.17. The method of claim 16 , wherein a majority of the plurality of partitions are provided within either the first or the second subset.18. The method of claim 15 , wherein the reactive component and the detection component comprise a first and a second antibody claim 15 , respectively.19. The method of claim 15 , wherein the detection component comprises one or more detectable labels.20. The method of claim 19 , wherein the one or more detectable labels comprises at least one of an optical label claim 19 , an enzymatic label claim 19 , and a radioactive label.21. The method of claim 20 , wherein the one or ...

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Номер записи: 50
17-02-2022 дата публикации

Flow cells

Номер: US20220048004A1
Автор: Arnaud Rival, Hui Han, Jeffrey S. Fisher, Justin FULLERTON, Lewis J. KRAFT, M. Shane Bowen, Mathieu LESSARD-VIGER, Tarun Kumar Khurana, Xi-jun CHEN, Yasaman Farshchi, Yir-Shyuan WU
Принадлежит: Illumina Inc

An example of a flow cell includes a substrate, a plurality of chambers defined on or in the substrate, and a plurality of depressions defined in the substrate and within a perimeter of each of the plurality of chambers. The depressions are separated by interstitial regions. Primers are attached within each of the plurality of depressions, and a capture site is located within each of the plurality of chambers.

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Номер записи: 51
17-02-2022 дата публикации

SYSTEMS AND METHODS TO ENHANCE CONSISTENCY OF ASSAY PERFORMANCE

Номер: US20220048025A1
Автор: Douglas Scott, HONKANEN Peter, Orencole Scott, Sullivan Travis
Принадлежит: AUSHON BIOSYSTEMS, INC.

Systems and methods are disclosed for enhancing the consistency of performance of assays, such as multiplexed assays, by printing features in a particular pattern, such that the outer edge of the pattern has a shape that is substantially similar to the shape of the test well. For example, the pattern is a ring pattern, such that the outer edge of the ring pattern is circular or oval along the bottom of multiplexed wells. The assay substrates prepared according to the methods described result in more accurate, precise, and sensitive chemical and/or biological analyses. 125.-. (canceled)26. A testing substrate comprising:a substrate material defining at least one well, the at least one well having a bottom surface, a center, and at least one side wall,the at least one side wall having a cross-sectional shape when lying in a plane parallel to the bottom surface; anda set of analysis features on the bottom surface in a pattern, characterized in that the pattern has multiple circular rings of analysis features, wherein each ring of the multiple rings of analysis features has a shape similar to the cross-sectional shape of the side wall,wherein for each ring of the multiple rings of analysis features, a distance from the outer edge of the at least one well to the center of each of the analysis features is about 20% to about 80% of a radius of the at least one well.27. The substrate of claim 26 , wherein for each ring of the multiple rings of analysis features claim 26 , all of the analysis features of the ring are arranged such that a distance from a center of each of the features to the center of the well is the same for each of the features.28. The substrate of claim 26 , wherein for each ring of the multiple rings of analysis features claim 26 , the analysis features of the ring are arranged such that an angle is formed by a first line that extends from the center of one feature to the center of the at least one well and a second line that extends from the center of an ...

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Номер записи: 52
17-02-2022 дата публикации

Enzyme quantification

Номер: US20220050108A1
Автор: Darren R. Link, Michael L. Samuels
Принадлежит: Bio Rad Laboratories Inc

The invention generally relates to methods for quantifying an amount of enzyme molecules. Systems and methods of the invention are provided for measuring an amount of target by forming a plurality of fluid partitions, a subset of which include the target, performing an enzyme-catalyzed reaction in the subset, and detecting the number of partitions in the subset. The amount of target can be determined based on the detected number.

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Номер записи: 53
30-01-2020 дата публикации

Methods, systems, computer readable media, and kits for sample identification

Номер: US20200032334A1
Автор: Earl Hubbell
Принадлежит: Life Technologies Corp

A method for sequencing a polynucleotide sample having a barcode sequence includes: introducing a series of nucleotides to the polynucleotide sample according to a predetermined order of nucleotide flows; obtaining a series of signals resulting from the introducing of nucleotides to the polynucleotide sample; and resolving the series of signals over the barcode sequence to render a flowspace string, wherein the flowspace string is a codeword of an error-correcting code that is (i) designed based on and adapted for use with the predetermined order of nucleotide flows, and (ii) capable of distinguishing any codeword in the error-correcting code from the other codewords in the error-correcting code in the presence of zero, one, and two errors.

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Номер записи: 54
05-02-2015 дата публикации

Microfluidic Transfer Pins

Номер: US20150038377A1
Автор: Brenan Colin John Herbert, Linton John, Madrone Leila
Принадлежит:

A liquid dispenser for a microfluidic assay system is described. The dispenser includes at least one transfer pin for transferring a microfluidic sample of liquid to a target receptacle. A pin tip at one end of the transfer pin is structured to cooperate with an opening in the target receptacle. The tip uses a high voltage potential to transfer the sample from the pin to the receptacle. 1. A microfluidic liquid dispenser for an assay system , the dispenser comprising:at least one transfer pin for transferring a microfluidic sample of liquid to a target receptacle; anda pin tip at one end of the transfer pin structured to cooperate with an opening in the target receptacle, the pin tip having a high voltage potential for transferring the sample from the at least one transfer pin to the target receptacle.2. A liquid dispenser according to claim 1 , wherein the target receptacle is a through-hole well in a platen array of wells.3. A liquid dispenser according to claim 1 , wherein the target receptacle is a closed-ended well in a platen array of wells.4. A liquid dispenser according to claim 1 , wherein the target receptacle includes hydrophilic walls regions that attract the sample.5. A liquid dispenser according to claim 1 , wherein the target receptacle includes an opening hydrophilic region surrounded by hydrophobic material.6. A liquid dispenser according to claim 1 , further comprising:a transfer pin array including a plurality of transfer pins for transferring a plurality of samples to a corresponding plurality of target receptacles.7. A liquid dispenser according to claim 6 , wherein individual transfer pins in the array are sequentially actuable.8. A liquid dispenser according to claim 6 , wherein at least one transfer pin in the array is independently positionable for alignment with respect to the opening of a target receptacle.9. A liquid dispenser according to claim 6 , wherein at least one individual transfer pin in the array is gravity-fed floating.10. A ...

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Номер записи: 55
11-02-2016 дата публикации

SOL-GEL KIT FOR PREPARING BIOCHIP AND METHODS FOR PREPARING BIOCHIP USING THE SAME

Номер: US20160038903A1
Автор: JO Minjoung, Lee Seram
Принадлежит:

A method of preparing a protein chip by gelation of a sol composition. In the method, a mixture of specific silicate monomers, such as SolB SolB and SolB SolBH, and a mixture of SolBS, distilled water and a detector protein are mixed sequentially, so that the gelation rate of the sol-composition can be delayed, thus inducing the stable gelation of the composition. Also, the biochip can be fabricated in a simple and easy manner by dispensing the sol composition by hand using an arrayer or a tool such as a pipette. In addition, a uniform biochip can be prepared by dispensing the sol composition, solution I (SolBH) and solution II (a mixture of buffer, SolBS, distilled water and a detector protein) sequentially onto a substrate without needing a conventional pretreatment process such as mixing. 1. A method for preparing a biochip by gelation of a sol composition , , the method comprising the steps of:{'sub': 2', '4', '3', '2, '(a) adding to a sol composition comprising SolB1, SolB2 and SolB3 and SolBH solution (solution I) selected from the group consisting of HCl, HSO, HNOand CHCOOH;'}(b) mixing the solution of step (a) with buffer SolBS and distilled water, and then stabilising the mixed solution at a temperature ranging from −20° C. to 4° C.; and(c) mixing the stabilized solution of step (b) with a solution containing a biological material which interacts with target biological material, dispensing the mixed solution onto a substrate and gelling the dispensed solution,wherein (i) said SolB1 is at least one first silicate monomer selected from the group consisting of methyltriethoxysilane (MTES), ethyltriethoxysilane (ETrEOS), sodium silicate, tetramethyl orthosilicate (TMOS), tetraethyl orthosilicate (TEOS) and tetramethoxysilicate (TMS);wherein (ii) said SolB2 is at least one second silicate monomer selected from the group consisting of 3-aminotrimethoxysilane (3-ATMS;), diglycerylsilane (DGS), methyltrimethoxysilicate (MTMS), polyglycerylsilicate (PGS), polyvinyl ...

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Номер записи: 56
08-02-2018 дата публикации

METHODS AND ARRAYS FOR PRODUCING AND SEQUENCING MONOCLONAL CLUSTERS OF NUCLEIC ACID

Номер: US20180037950A1
Автор: Bai Jingwei, Beierle John M., Boutell Jonathan Mark, Boyanov Boyan, Gunderson Kevin L., KELLINGER Matthew William, Maisinger Klaus, Rigatti Roberto, Rogert Bacigalupo Maria Candelaria
Принадлежит:

The present disclosure relates to the field of molecular biology and more specifically to microarrays and methods. 1. A microarray comprising:a) a substrate comprising at least one well, a surface surrounding the well and an inner well surface;b) a first layer covering the inner well surface and comprising at least one first capture primer pair; andc) a second layer covering the first layer and the surface surrounding the well.2. The microarray of claim 1 , wherein the first layer does not cover the surface surrounding the well.3. The microarray of claim 1 , wherein the first layer at least partially covers the inner well surface.4. The microarray of claim 1 , wherein the at least one well is a plurality of wells.5. The microarray of claim 4 , wherein the plurality of wells are spaced at a pitch of about 700 nm.6. The microarray of claim 1 , wherein the diameter of the well is less than about 1 μm.7. (canceled)8. The microarray of claim 6 , wherein the diameter of the well is between about 100 nm and 400 nm.9. (canceled)10. The microarray of claim 1 , wherein the first layer comprises a first polymer coating claim 1 , and wherein the second layer optionally comprises a second polymer coating.11. The microarray of claim 10 , wherein the first polymer coating comprises poly(N-(5-azidoacetamidylpentyl)acrylamide-co-acrylamide (PAZAM) claim 10 , and wherein the second polymer coating comprises PAZAM or silane free acrylamide (SFA).12. (canceled)13. (canceled)14. The microarray of claim 1 , wherein the at least one first capture primer pair is a plurality of first capture primer pairs claim 1 , and wherein primers of the at least one first capture primer pair optionally comprise a universal capture region.15. (canceled)16. The microarray of claim 14 , wherein the first primer of the at least one first capture primer pair comprises an Illumina® P5 primer nucleotide sequence and the second primer of the at least one first capture primer pair comprises an Illumina® P7 ...

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Номер записи: 57
12-02-2015 дата публикации

LOW-CHLORIDE LIPF6

Номер: US20150044118A1
Автор: BOLL Matthias, EBENBECK Wolfgang, KUCKERT Eberhard
Принадлежит:

The present invention relates to a process for preparing low-chloride LiPF, in particular low-chloride LiPFsolutions, from PClas starting material and via PClas intermediate product, and also to apparatus to be used for this. 1. Process for preparing LiPFsolutions in an organic solvent , or a mixture of two or more organic solvents , proceeding from PCl , which is first reacted continuously in the gas phase with HF to form a PF-containing reaction mixture which in turn is reacted continuously in the gas phase with Clinitially to form a PClF-containing reaction mixture and with additional HF to form a PF-containing reaction mixture , characterized in that the PF-containing reaction mixture is finally reacted in a fixed bed reactor or fluidized bed reactor over LiF mouldings or with an LiF powder and/or an LiFxHF adduct , and the reaction product is washed with an organic solvent out of the fixed bed reactor or the fluidized bed reactor and isolated.2. Process according to claim 1 , characterized in that the PF-containing reaction mixture is temperature regulated to temperatures of −50 to +200° C. before entry into the fixed bed reactor or fluidized bed reactor.3. Process according to or claim 1 , characterized in that LiF mouldings used in the fixed bed reactor or in the fluidized bed reactor are prepared beforehand by extrusion from a mixture of LiF and water wherein the solids content is in the range from 20 to 95 wt % and after extrusion these mouldings are dried at temperatures of 50 to 200° C. and they merely retain a water content of 0.05 to 5 wt % claim 1 , wherein the water content is determined by the method of Karl Fischer.4. Process according to claim 3 , characterized in that the LiF is employed in the form of mouldings or in the form of fine particles having a particle size distribution in the range from 5 to 500 μm.54. Process according to - claims 1 , characterized in that the gas mixture emerging from the fixed bed reactor or the fluidized bed is ...

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Номер записи: 58
07-02-2019 дата публикации

DEVICE AND METHOD FOR HYDROGEN PRODUCTION WITH WASTE ALUMINUM, AND METHOD FOR HYDROGEN PRODUCTION WITH ALUMINUM

Номер: US20190039888A1
Автор: Ho Ching-Yuan
Принадлежит: CHUNG YUAN CHRISTIAN UNIVERSITY

A device for hydrogen production with waste aluminum includes a treatment apparatus for waste aluminum and a reaction tank. The apparatus includes a first crusher, a pickling tank, and a second crusher. The first crusher is for preliminarily crushing waste aluminum to obtain first aluminum chips. The pickling tank is for receiving and pickling the first aluminum chips crushed by the first crusher. The second crusher is for receiving and fine crushing the first aluminum chips to obtain second aluminum chips. The second aluminum chips are received by the reaction tank and then hydrolyzed with an alkaline solution in the reaction tank to produce hydrogen. Since waste aluminum is used as the raw material of hydrogen production, and a specific device is used for waste aluminum treatment, so the effects of recovering waste metal, reducing environmental damage, and saving costs can be achieved at the same time. 1. A device for hydrogen production with a waste aluminum , comprising: a first crusher for preliminarily crushing the waste aluminum to obtain first aluminum chips;', 'a pickling tank for receiving and pickling the first aluminum chips crushed by the first crusher; and', 'a second crusher for receiving and performing a fine crushing on the first aluminum chips pickled by the pickling tank to obtain second aluminum chips; and, 'a treatment apparatus for the waste aluminum, comprisinga reaction tank for receiving the second aluminum chips obtained from the treatment apparatus to hydrolyze the second aluminum chips with an alkaline solution in the reaction tank.2. The device for hydrogen production with the waste aluminum of claim 1 , wherein the treatment apparatus for the waste aluminum further comprises a stamping device for receiving and stamping the second aluminum chips crushed by the second crusher.3. The device for hydrogen production with the waste aluminum of claim 1 , further comprises an anti-corrosion layer is disposed on an inner surface of the reaction ...

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Номер записи: 59
18-02-2016 дата публикации

STABILISATION FEATURES

Номер: US20160045883A1
Автор: BARRETT Brian, McCabe Mark, Ryan Caitriona, Tuohy Patrick
Принадлежит: GENCELL BIOSYSTEMS LTD.

Devices, systems and methods for making and handling liquid samples are disclosed. 1. A stabilization feature for immobilizing a composite liquid cell , the stabilization feature comprising:a hydrophobic wall defining a vessel; the vessel defines a central portion sized and shaped to snugly contain a composite liquid of a predetermined size;', 'the vessel defines a vertical axis; and', 'the vessel defines at least one channel portion adapted to flow gas away from the central portion., 'wherein2. The stabilization feature of wherein the vessel has a cross-sectional shape in a plane perpendicular to the vertical axis claim 1 , and the cross-sectional shape is constant along the vertical axis.3. The stabilization feature of wherein the vessel has a cross-sectional shape in a plane perpendicular to the vertical axis claim 1 , and the cross-sectional shape is not constant along the vertical axis.4. The stabilization feature of wherein the vessel is tapered so that the cross-sectional shape is larger higher along the vertical axis.5. The stabilization feature of wherein the central portion is substantially circular.6. The stabilization feature of wherein the at least one channel portion is offset horizontally from the central portion.7. The stabilization feature of wherein the at least one channel portion is a plurality of channel portions.8. The stabilization feature of wherein the plurality of channel portions is two channel portions spaced 180 degrees apart from one another around the vertical axis.9. The stabilization feature of wherein the plurality of channel portions is four channel portions spaced 90 degrees apart from one another around the vertical axis.10. The stabilization feature of wherein the at least one channel portion is substantially circular.11. The stabilization feature of wherein the at least one channel portion is substantially rectangular.12. The stabilization feature of wherein the cross-sectional shape is substantially rhomboid.13. A ...

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Номер записи: 60
15-02-2018 дата публикации

METHOD FOR TREATING WATER WITH CHLORINE DIOXIDE

Номер: US20180044180A1
Автор: ANDRE Neil, BELISLE Randy D., BURKE Adrian Alan, GARRISON Peter, Glynn Scott C., HULSMAN William J., TROTTIER Michael
Принадлежит: International Dioxcide, Inc.

A method for treating water with chlorine dioxide wherein the reactor is contained inside of the water supply line being treated and an eductor is used to draw in the chemical precursors. The method offers facilitated chlorine dioxide (ClO ) generation and safer operation over wider ClOmass flow capacity, thus offering a more adaptable system for CLOtreatments. Noise reduction and ease-of-use versus traditional eductor-based ClOgenerators are additional benefits from using this method. 1. A method for ClOtreatment that uses an eductor-based reactor assembly to expand the ClOflow capacity comprising:{'sub': '2', 'an eductor to provide flows of precursor chemicals to generate the ClO'}{'sub': 2', '2, 'a mixing zone to ensure the ClOis generated at safe operating pressures below explosive limits of the ClO;'}{'sub': '2', 'a pipe to which the reactor assembly is mounted that allows for containment of the eductor inside the process stream and direct treatment of the process stream with the ClO;'}a means to provide motive water supply for the eductor;{'sub': '2', 'a control system that monitors precursor chemical flow rates and process flow rates to ensure that proper dilution and safe ClOdosage is being applied to the process stream being treated.'}2. The method according to whereby the precursors are acid and sodium chlorite.3. The method according to whereby the precursors are acid claim 1 , sodium hypochlorite claim 1 , and sodium chlorite.4. The method according to whereby the precursors are chlorine and sodium chlorite.5. The method according to wherein a flushing zone is an additional component of the reactor assembly that prevents ClOfrom accumulating within the process line and reactor assembly volume by continuously flushing volume outside of the eductor.6. The method according to wherein the reactor assembly is of modular design to accommodate interchangeable reactor assemblies for variable chlorine dioxide production capacity and turn down ratio.7. The method ...

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Номер записи: 61
03-03-2022 дата публикации

SYSTEMS AND METHODS FOR TAGGING AND ACOUSTICALLY CHARACTERIZING CONTAINERS

Номер: US20220062907A1
Автор: Datwani Sammy, HINKSON Stephen, SACKMANN Eric
Принадлежит:

Embodiments of the present invention provide systems and methods for tagging and acoustically characterizing containers. 1providing the container, the container: originating from within a mold and comprising at least one vertical sidewall; and a bottom coupled to the at least one vertical sidewall wherein the bottom has an interior side, an exterior side, and a thickness and includes a plurality of recesses, grooves, or protrusions on the exterior side for acoustic characterization of the container;transmitting an acoustic signal toward the bottom;receiving a plurality of reflections of the acoustic signal, wherein the plurality of reflections comprises a first reflection from a first subset of the plurality of recesses, grooves, or protrusions a second reflection from a second subset of the plurality of recesses, grooves, or protrusions, the first reflection having a first time of flight and the second reflection having a second time of flight; andcharacterizing the container based on the first and second times of flight, wherein the characterizing comprises determining a characteristic of the container,wherein (a) the characteristic is associated with a location in the mold from which the container originates or (b) the mold is one of a plurality of molds and the characteristic is associated with the mold.. A method for acoustically characterizing a container configured to hold a fluid, the method comprising: This application is a continuation of U.S. patent application Ser. 16,705,807, filed Dec. 6, 2019, which is a continuation of U.S. patent application Ser. 15/289,692, filed Oct. 10, 2016, which claims the benefit of U.S. Provisional Patent Application No. 62/240,412, filed Oct. 12, 2015, and all entitled “Systems and Methods for Tagging and Acoustically Characterizing Containers,” the entire contents of all applications are incorporated by reference herein.This application relates to tagging containers, such as containers formed using injection molding. But ...

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Номер записи: 62
14-02-2019 дата публикации

GEL PATTERNED SURFACES

Номер: US20190046943A1
Автор: Barnard Steven M., Bowen M. Shane, Brown Andrew A., George Wayne N., Rogert Bacigalupo Maria Candelaria, Tsay James
Принадлежит:

An example method includes contacting a substrate coated with a sol-gel material with a stamp that includes a plurality of protruding features. While contacting the coated sol-gel material with the stamp, the example method further includes curing the coated sol-gel material so as to form a patterned sol-gel layer that includes a plurality of wells. The stamp is separated from the patterned sol-gel layer. 128-. (canceled)29. A method comprising:contacting a substrate coated with a sol-gel material with a stamp that includes a plurality of protruding features;while contacting the coated sol-gel material with the stamp, curing the coated sol-gel material so as to form a patterned sol-gel layer that comprises a plurality of wells; andseparating the stamp from the patterned sol-gel layer.30. The method of claim 29 , further comprising forming the coated substrate by coating the substrate with the sol-gel material claim 29 , wherein the coating is performed by spin-coating claim 29 , dipping claim 29 , or spray-coating.31. The method of claim 29 , wherein the curing the coated sol-gel material is performed by exposing the coated sol-gel material to light.32. The method of claim 29 , wherein the curing the coated sol-gel material is performed by exposing the coated sol-gel material to heat.33. The method of claim 29 , further comprising claim 29 , after separating the stamp from the patterned sol-gel layer claim 29 , sintering the patterned sol-gel layer.34. The method of claim 29 , further comprising depositing a gel material in the wells claim 29 , wherein the gel material is adapted to promote capture and/or amplification of target nucleic acids.35. The method of claim 34 , where depositing the gel material in the wells comprises:coating the gel material on the patterned sol-gel layer such that the gel material enters the wells and coats interstitial regions between the wells; andpolishing the patterned sol-gel layer so as to remove the gel material from the ...

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Номер записи: 63
14-02-2019 дата публикации

SCREENING ASSAYS AND METHODS

Номер: US20190046944A1
Автор: Love J. Christopher, Ploegh Hidde L., Ronan Jehnna
Принадлежит:

Screening assays and methods of performing such assays are provided. In certain examples, the assays and methods may be designed to determine whether or not two or more species can associate with each other. In some examples, the assays and methods may be used to determine if a known antigen binds to an unknown monoclonal antibody. 1. A printed microarray of unknown cell-derived products , each position of said array comprising a deposit corresponding to a composition of a single cell , said deposit being less than 100 micrometers in diameter.2. The microarray of claim 1 , wherein said deposit is a secreted cell-derived product selected from the group consisting of an antibody claim 1 , cytokine claim 1 , chemokine claim 1 , and inflammatory mediator.3. The microarray of claim 1 , wherein said microarray comprises a capture ligand for a class of secreted products.4. The microarray of claim 3 , wherein said capture ligand hinds to a single immunoglobulin isotype. This application claims priority to U.S. Ser. No. 60/717,976 filed Sep. 16, 2005, which is incorporated herein by reference in its entirety.This invention was funded in part by the U.S. Government under grant numbers NAKFI Nano08 awarded by the National Academy of Sciences and/or grant 5R01A1034893-1 awarded by the National Institutes of Health. The Government has certain rights in the invention.The invention relates to screening assays and methods to identify secreted products.Assays exist to identify compounds or molecules of interest that may be involved in a disease process or other condition or in treating a disease process or condition. Existing assays have some drawbacks. One significant drawback is the time required to screen for many compounds or molecules. Another drawback is that it may not be possible to recover the compound or molecule post-screening. There remains a need for better screening assays and methods.The invention provides a printed microarray of unknown cell-derived products. Each ...

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Номер записи: 64
08-05-2014 дата публикации

Micro-reactor array

Номер: US20140128281A1
Автор: Guanbin Zhang, Jia Wang, Jing Cheng, Kaijun Zhao, Shuang AN, Tao Guo, TAO Sheng, Wanli Xing
Принадлежит: CapitalBio Corp, TSINGHUA UNIVERSITY

The present disclosure provides a cover sheet for a microarray reaction device. In one aspect, the present cover sheet or device ensures the reaction units/volumes are stable and/or consistent among assay samples and assay runs, allowing samples (e.g., reaction solutions) to be conveniently added and distributed uniformly.

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Номер записи: 65
22-02-2018 дата публикации

METHOD FOR ASSEMBLY OF ANALYTE FILTER ARRAYS USING BIOMOLECULES

Номер: US20180051390A1
Автор: John Jeremy J., Mitchell Daniel R., Murphy George L., Savoy Steve M.
Принадлежит: NANOHMICS, INC.

Analyte filter arrays and methods for making an analyte filter array are provided. The arrays are formed using a dispersion of filter particles having selected moieties attached to the surface of the particles and a microarray having complementary moieties formed in an array on a substrate, such that each filter particle is attached to a selected region of the microarray. The moiety on the substrate may be RNA or DNA or other molecule. The substrate may be a surface of a detector array, a membrane that may be placed in registration with the detector array or a stamp used to transfer the filter array to a detector array. 1. A method of making a filter particle array for filtering molecules or ions in a mixture comprising:(a) exposing a plurality of filter particles to a detector array, the array having a plurality of addressable regions, wherein the plurality comprises a set of selected filter particles having binding moieties attached thereto, and wherein exposing causes binding of the binding moieties to complementary anchor biomolecules attached to a selected addressable region, thereby binding the set of selected filter particles to the selected addressable region; and(b) removing unbound filter particles from the detector array.2. The method of wherein the detector array is a chemiresistive semiconductor detector array.3. The method of wherein the chemiresistive semiconductor detector array comprises an array of nanoimprinted metal oxide semiconductors.4. The method of further comprising providing the detector array.5. The method of wherein the set of filter particles are selected to filter ionic species or gas species in a mixture.6. The method of wherein the detector array is configured to respond electrically to the interaction of an ion or gas at the detector surface.7. The method of wherein steps (a) and (b) are repeated at least once.8. The method of wherein at least two selected addressable regions have different anchor biomolecules claim 1 , and wherein ...

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Номер записи: 66
03-03-2016 дата публикации

METHOD OF FABRICATING CELL ARRAYS AND USES THEREOF

Номер: US20160059203A1
Автор: Petcavich Robert John
Принадлежит:

The present disclosure provides a fabrication process that results in creating large arrays of living cells, such as stem cells, which are subsequently exposed to nanoliter quantities of compounds to test the efficacy on cellular metabolism. 1. A method of fabricating and dosing a stem cell array , comprising:providing a mixture of cells and a UV curable hydrogel matrix;applying a volume of the mixture in N×N array to a support and optionally providing for a specific geometry for the applied volume;exposing the support to UV light to cure each volume in the array and optionally incubating the array under conditions that provide for cell viability; andcontacting the array with one or more compounds.2. The method of wherein the cells are stem cells.3. The method of wherein the cells are iPSCs.4. The method of wherein the cells are differentiated cells.5. The method of claim wherein the support is a rigid support.6. The method of wherein the support is a flexible support.7. The method of wherein the support comprises glass claim 1 , ceramic or a polymer sheet or film.8. The method of wherein the array is coated with an adhesion polymer.9. The method of wherein the specific geometry is provided by a mold or by microstructures in the array.10. The method of wherein the one or more compounds are applied using ultrasonic deposition.11. A method of fabricating a stem cell array claim 1 , comprising:mixing stem cells into or onto a UV curable hydrogel matrix;coating the mixture onto a support to provide a N x N array of the hydrogel stem cell mixture with a specific predetermined geometry; andapplying UV light to cure the mixture in the presence of a micro mold template.12. The method of further comprising incubating the cells in nutrient solution and optionally exposing the array to one or more compounds.13. The method of wherein the one or more compounds is/are applied via ultrasonic deposition.14. The method of wherein the cells are iPSC cardiomyocytes.15. The method of ...

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Номер записи: 67
01-03-2018 дата публикации

DEVICES AND METHODS FOR PROGRAMMABLE MANIPULATION OF PIPETTES

Номер: US20180056286A1
Автор: Horak Giorgio, Jordan Antoine, Zucchelli Piero
Принадлежит:

The present invention is directed generally to devices and methods for manipulating laboratory pipettes in a programmable manner. The present invention is directed to an apparatus and methods for allowing a user to instruct the device to perform a specific process; identifying the type, location and identity of the consumables to be used; manipulating a plurality of pipettes for performing the liquid handling; monitoring the process during and after its execution; generating a detailed report for the plurality of actions. Other aspects of this invention include optimization of the liquid dispensing performances of a pipette; monitoring and controlling individual actions by means of vision; virtualization of the protocol definition by means of a reality augmented software interface; integration of the system in a conventional laboratory environment workflow. 1. An apparatus for processing biological or chemical fluids , comprisingat least one pipette, wherein said at least one pipette is configured to allow for a pipette volume to be determined by vision feedback;at least one mechanical arm to manipulate at least one pipette said manipulation is assisted by computer vision feedback; anda software interface governing the manipulation of the pipettes.25.-. (canceled)6. The apparatus according to claim 1 , wherein the software interface allows for programming of a liquid handling protocol.7. (canceled)8. The apparatus according to claim 1 , wherein the arm manipulation includes movement of the at least one pipette claim 1 , tip insertion and ejection claim 1 , setting of the desired volumes claim 1 , aspiration and dispensing of liquids.9. The apparatus according to claim 8 , wherein the manipulation of the at least one pipette by sequences of aspiration and dispenses allows for liquid mixing.1036.-. (canceled)37. A method for improving the volumetric reproducibility of a pipette in a liquid handling android claim 8 , comprising:actuating the thumb action for aspirating ...

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Номер записи: 68
21-02-2019 дата публикации

FLUIDIC DEVICES WITH BEAD WELL GEOMETRIES WITH SPATIALLY SEPARATED BEAD RETENTION AND SIGNAL DETECTION SEGMENTS AND RELATED METHODS

Номер: US20190054470A1
Автор: Henley William Hampton, Ramsey John Michael
Принадлежит:

A fluidic device includes a plurality of reaction wells, typically in a dense array, with at least one bead retention segment in fluid communication with and spatially separated from at least one signal detection segment. A respective bead retention segment can be configured to hold a single bead, which can have a reagent attached thereto. 1. A fluidic device comprising:a plurality of reaction wells characterized in that the reaction wells have at least one bead retention segment and at least one spatially separated signal detection segment in fluid communication with the at least one bead retention segment.2. The device of claim 1 , wherein the device is a microfluidic chip and the reaction wells are provided as an array of reaction wells.3. The device of claim 1 , wherein the reaction wells have a volumetric capacity of between 1 aL to 1 μL.4. The device of claim 1 , wherein the at least one bead retention segment is sized and configured to hold only a respective single bead claim 1 , and wherein at least one of the at least one separate signal detection segment has an end portion that resides a distance L of between 0.3× to about 200× of a diameter of a target bead claim 1 , typically between 1 μm to 1 mm claim 1 , from one or more of the at least one bead retention segment claim 1 , optionally between 1 μm to 10 μm.5. The device of claim 1 , wherein the at least one bead retention segment is sized and configured to hold a microspherical bead with a diameter of between 100 nm to 1 mm claim 1 , and wherein the at least one bead retention segment has a width that is between 101% to 195% of the diameter of a respective bead held therein.6. The device of claim 1 , wherein the separate at least one signal detection segment comprises an elongate channel that has a width that is less than a width of an adjacent bead retention segment.7. The device of claim 1 , wherein the at least one bead retention segment is a single bead retention segment.8. The device of claim 1 , ...

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Номер записи: 69
10-03-2022 дата публикации

Manufacturing a flowcell with a planar waveguide

Номер: US20220075263A1
Автор: Dajun Yuan, M. Shane Bowen, Zhong Mei
Принадлежит: Illumina Inc

Provided in one example is a method of manufacturing a flowcell that includes: forming a core layer, the core layer disposed between a substrate and a nanowell layer, the nanowell layer having nanowells to receive a sample, the core layer having a higher refractive index than the substrate and the nanowell layer; and forming a grating to couple light to the core layer.

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Номер записи: 70
04-03-2021 дата публикации

MICROARRAY SYNTHESIS AND ASSEMBLY OF GENE-LENGTH POLYNUCLEOTIDES

Номер: US20210062185A1
Автор: Oleinikov Andrew V.
Принадлежит: Gen9, Inc.

There is disclosed a process for in vitro synthesis and assembly of long, gene-length polynucleotides based upon assembly of multiple shorter oligonucleotides synthesized in situ on a microarray platform. Specifically, there is disclosed a process for in situ synthesis of oligonucleotide fragments on a solid phase microarray platform and subsequent, “on device” assembly of larger polynucleotides composed of a plurality of shorter oligonucleotide fragments. 1. (canceled)2. A method for producing a polynucleotide comprising a target sequence , the method comprising: [ is identical to an overlapping sequence region of another double-stranded oligonucleotide in the plurality of double-stranded oligonucleotides; and', 'comprises a Type II restriction endonuclease cleavage site; and, '(i) an internal sequence identical to a portion of the target sequence, wherein the internal sequence, at one or both ends, comprises an overlapping sequence region that, '(ii) one or more flanking sequences, wherein each flanking sequence comprises a Type II restriction endonuclease recognition site corresponding to the Type II restriction endonuclease cleavage site of the internal sequence;, '(a) providing a plurality of double-stranded oligonucleotides, each double-stranded oligonucleotide comprising(b) digesting the plurality of double-stranded oligonucleotides with a Type II restriction endonuclease that recognizes the Type II restriction endonuclease recognition site; and(c) ligating the digested oligonucleotides to produce the polynucleotide comprising the target sequence.3. The method of claim 2 , wherein each double-stranded oligonucleotide in (a) comprises a flanking sequence at each end.4. The method of claim 2 , wherein the plurality of double-stranded oligonucleotides are digested in (b) with a Type IIS restriction endonuclease.5. The method of claim 2 , wherein the Type IIS restriction endonuclease includes at least one of MylI claim 2 , BspMI claim 2 , BsaXI claim 2 , BsrI ...

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Номер записи: 71
05-03-2015 дата публикации

MULTI-WELL MICROPATTERNING BY ABLATION

Номер: US20150065389A1
Автор: BHATIA Sangeeta N., Eddington David T.
Принадлежит: Massachusetts Institute of Technology

The present invention is drawn to the generation of micropatterns of biomolecules and cells on standard laboratory materials through selective ablation of a physisorbed biomolecule with oxygen plasma. In certain embodiments, oxygen plasma is able to ablate selectively physisorbed layers of biomolecules (e.g., type-I collagen, fibronectin, laminin, and Matrigel) along complex non-linear paths which are difficult or impossible to pattern using alternative methods. In addition, certain embodiments of the present invention relate to the micropatterning of multiple cell types on curved surfaces, multiwell plates, and flat bottom flasks. The invention also features kits for use with the subject methods. 1. A method of forming a micropatterned substrate , comprising the steps of:adsorbing molecules onto a surface of a substrate, thereby forming a coated surface of the substrate;comprising a micropatterned etch mask onto the coated surface of said substrate; andexposing the compressed micropatterned etch mask and coated surface of the substrate to a gas plasma for a period of time, thereby ablating the exposed surfaces of the substrate.2. The method of claim 1 , further comprising rinsing and drying said coated surface after the adsorbing step.3. The method of claim 1 , wherein said exposing step is carried out in a plasma asher.4. The method of claim 1 , wherein the micropatterned etch mask is one solid elastomericpiece.5. The method of claim 1 , wherein the micropatterned etch mask comprises a plurality of pillars.6. The method of claim 1 , wherein the micropatterned etch mask comprises chrome or elastomeric poly(dimethylsiloxane) or rubber or plastic.7. The method of claim 1 , wherein the adsorbed molecules are different.8. The method of claim 7 , wherein the different molecules each have a different pattern.9. The method of claim 1 , wherein the micropatterned etch mask comprises plastic.10. The method of claim 1 , wherein the micropatterned etch mask comprises an about ...

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Номер записи: 72
28-02-2019 дата публикации

Chemical liquid dispensing apparatus and chemical liquid discharging device

Номер: US20190060936A1
Автор: Ryutaro Kusunoki, Satoshi Kaiho, Seiya Shimizu, Shuhei Yokoyama
Принадлежит: Toshiba TEC Corp

A liquid discharging device to be used with a liquid dispensing apparatus includes a discharging portion configured to discharge a liquid based on a control signal from the liquid dispensing apparatus on which the liquid discharging device is mounted, and a sheet material having a characteristic configured to be changed by the liquid dispensing apparatus after a discharge of the liquid by the discharging portion.

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Номер записи: 73
17-03-2022 дата публикации

NANOSCALE BIOCHEMICAL SAMPLE PREPARATION AND ANALYSIS

Номер: US20220080420A1
Автор: Kelly Ryan T., Smith Richard D., Zhu Ying
Принадлежит: BATTELLE MEMORIAL INSTITUTE

Provided herein are methods and systems for biochemical analysis, including compositions and methods for processing and analysis of small cell populations and biological samples (e.g., a robotically controlled chip-based nanodroplet platform). In particular aspects, the methods described herein can reduce total processing volumes from conventional volumes to nanoliter volumes within a single reactor vessel (e.g., within a single droplet reactor) while minimizing losses, such as due to sample evaporation. 1. A platform for biological sample preparation , comprising:{'sup': '2', '#text': 'a substrate comprising at least one reactor vessel having one or more hydrophilic surfaces configured for containment of a biological sample, wherein the hydrophilic surfaces have a non-zero total surface area less than 25 mm;'}a spacer containing an aperture, wherein the aperture is dimensioned to surround the at least one reactor vessel when the spacer is positioned on the substrate; anda cover positioned on the spacer.2. The platform of claim 1 , further comprising a membrane interposed between the spacer and the cover claim 1 , the membrane configured to form a gas-tight seal between the spacer and the cover to minimize evaporation.3. The platform of claim 1 , wherein the platform is formed from a material that is substantially optically transparent.4. The platform of claim 1 , wherein the platform is formed from glass.5. The platform of claim 1 , further comprising at least one hydrophobic surface surrounding the at least one reactor vessel.6. The platform of claim 1 , further comprising at least two reactor vessels claim 1 , wherein the at least two reactor vessels are separated by a hydrophobic surface.7. The platform of claim 1 , wherein the hydrophilic surface is formed on an upper surface of a pillar and defines the lateral boundary of the least one reactor vessel.8. The platform of claim 1 , wherein the at least one reactor vessel is a well having a depth extending below a ...

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Номер записи: 74
09-03-2017 дата публикации

Fluid Deposition Apparatus and Method

Номер: US20170065958A1
Автор: Cheng Chun-Wen, Lai Fei-Lung, Liu Yi-Shao, Peng Jung-Huei, Tsai Shang-Ying
Принадлежит:

The present disclosure relates to a method of depositing a fluid onto a substrate. In some embodiments, the method may be performed by mounting a substrate to a micro-fluidic probe card, so that the substrate abuts a cavity within the micro-fluidic probe card that is in communication with a fluid inlet and a fluid outlet. A first fluidic chemical is selectively introduced into the cavity via the fluid inlet of the micro-fluidic probe card. 1. A method of depositing a fluid onto a substrate , comprising:mounting a substrate to a micro-fluidic probe card, so that the substrate abuts a cavity within the micro-fluidic probe card that is in communication with a fluid inlet and a fluid outlet; andselectively introducing a first fluidic chemical to the cavity via the fluid inlet of the micro-fluidic probe card.2. The method of claim 1 , further comprising:dismounting the micro-fluidic probe card from the substrate.3. The method of claim 1 , further comprising:removing the first fluidic chemical from the cavity via the fluid outlet of the micro-fluidic probe card; andselectively introducing a second fluidic chemical to the cavity via the fluid inlet of the micro-fluidic probe card.4. The method of claim 1 , further comprising:selectively introducing a drying agent, which is configured to dry the substrate, to the cavity via the fluid inlet of the micro-fluidic probe card.5. The method of claim 4 , wherein the drying agent comprises nitrogen gas.6. The method of claim 1 , further comprising:performing a performance check to determine if the first fluidic chemical has been deposited onto the substrate prior to dismounting the micro-fluidic probe card from the substrate.7. The method of claim 6 , wherein the micro-fluidic probe card comprises a transparent surface positioned between the substrate and a performance monitoring element that is configured to perform the performance check.8. The method of claim 7 , wherein performing the performance check comprises:directing light ...

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Номер записи: 75
11-03-2021 дата публикации

LOADING NUCLEIC ACIDS ONTO SUBSTRATES

Номер: US20210069664A1
Автор: Benitez-Marzan Jaime Juan, LIN Steven, Popovich Natasha, Sheikholeslami Sassan, Sun Lei, Vedula Aparna
Принадлежит:

Methods, compositions, and systems for distributing nucleic acids into array regions are provided. The methods, compositions, and systems utilize nucleic acid condensing agents to increase efficiency of distribution of the nucleic acids into the array regions. Various methods for facilitating distribution of the nucleic acids to the array regions are provided. 17-. (canceled)8. A method for distributing polymerase-template complexes into a plurality of nanoscale wells , the method comprising:providing a surface comprising the plurality of nanoscale wells;contacting polymerase-template complexes in solution with polyethylene glycol (PEG) and a salt comprising a cation to compact the templates of the polymerase-template complexes from a random coil into a compacted toroidal, spherical, or globular form, thereby providing a solution comprising compacted polymerase-template complexes, wherein the compacted polymerase-template complexes are not bound to beads;andexposing the surface to the solution, whereby the compacted polymerase-template complexes diffuse into the nanoscale wells, thereby distributing the compacted polymerase-template complexes into the nanoscale wells.9. The method of claim 8 , wherein the solution comprises PEG 8000.10. The method of claim 8 , wherein the solution comprises 2.5-25 mM PEG 8000.11. The method of claim 8 , wherein the solution comprises 5-15 mM PEG 8000.12. (canceled)13. The method of claim 8 , wherein the solution comprises a monovalent cation at 50 to 500 mM.14. The method of claim 8 , wherein the solution comprises a monovalent cation at 100 to 300 mM.15. (canceled)16. The method of claim 15 , wherein the solution comprises a divalent cation at 0.05 to 10 mM.17. The method of claim 8 , wherein the solution comprises PEG 8000 and K.18. The method of claim 8 , wherein the solution comprises PEG 8000 claim 8 , K claim 8 , and Sr.19. The method of claim 8 , wherein the solution comprises 5-15 mM PEG 8000 and 100-300 mM K.20. The method ...

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Номер записи: 76
09-03-2017 дата публикации

AUTOMATIC GAS VALVE CONTAINER HOLDER FOR CHEMICAL SYNTHESIS

Номер: US20170066798A1
Автор: Carlsson Daniel, Gamberg Mangus, Olovsson Bjorn Markus, Osmark Mathias
Принадлежит:

The invention relates to a container holder comprising a main body which in turn comprises a gas inlet a solution liquid outlet a gas control valve through which a gas enters the container from the gas inlet; and a sealing means for the container, which sealing means includes a passageway for the input of gas and output of a solution in the container via an egress tube wherein when the container is connected to the container holder through the sealing means, the gas control valve opens automatically, and when the container is disconnected the gas control valve is closed automatically. The invention further relates to a container panel which includes two or more container holders. Also disclosed are methods of using these containers and container panels for synthesizing a polypeptide. 1. A container holder , comprising a main body which in turn comprises:(a) a gas inlet;(b) a solution outlet;(c) a gas control valve which allows a gas to enter the container from the gas inlet; and(d) a sealing means for sealing the container in use, which sealing means includes a passageway for the input of the gas and for the egress of a liquid in the container;wherein, when the container is sealingly connected to the container holder by the sealing means, the gas control valve opens automatically, and when the container is disconnected from the container holder the gas control valve is closed automatically.2. The container holder of claim 1 , wherein the gas control valve is a spring valve operable by means of a plate claim 1 , in turn displaced be the act of sealingly connecting the container to the container holder.3. The container holder of claim 1 , wherein the main body includes an internally threaded aperture and wherein the sealing means comprises a threaded part complementary to the threaded aperture and a plate claim 1 , wherein when the container is connected with the threaded part claim 1 , the plate opens the gas control valve.4. The container holder of claim 2 , wherein ...

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Номер записи: 77
12-03-2015 дата публикации

Well plate and suction device provided with well plate

Номер: US20150072405A1
Автор: Saburo Ito
Принадлежит: Yamaha Motor Co Ltd

A well plate is formed with a well for holding a subject to be sucked by a suction nozzle on an inner bottom part and storing liquid and, a clearance forming member for forming a clearance to allow the liquid to flow in a state where a tip part of the suction nozzle is inserted into and held in contact with the well is provided in the well. According to the present invention, the clearance enabling the liquid to flow is formed even in the state where the tip part of the suction nozzle is inserted into and held in contact with the well in sucking the subject held in the well by the suction nozzle. The suction nozzle can suck the liquid around through the clearance and the subject held in the well is efficiently sucked through the suction port along the flow of the sucked liquid.

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Номер записи: 78
12-03-2015 дата публикации

Systems and Methods for Loading Liquid Samples

Номер: US20150072897A1
Автор: Evan W. Foster, Gary Lim, James C. Nurse, Michael C. Pallas, Theodore E. Straub
Принадлежит: Life Technologies Corp

A sample loader for loading a liquid sample into a plurality of reaction sites within a substrate is provided. The sample loader includes a first blade, and a second blade coupled to the first blade. The sample loader further comprises a flow path between the first blade and second blade configured to dispense a liquid sample to a substrate including a plurality of reaction sites. Further, in various embodiments the liquid sample has an advancing contact angle of 85+/−15 degrees with the first and second blade. Furthermore, loading of the liquid sample dispensed from the flow path to the plurality of reaction sites may be based on capillary action.

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Номер записи: 79
09-03-2017 дата публикации

DEVICE AND METHOD FOR PROCESSING TARGET COMPONENT IN TUBE

Номер: US20170067923A1
Автор: OHASHI Tetsuo
Принадлежит:

The present invention provides a small and low running-cost device capable of minimizing the generation of contamination sources as much as possible while performing a series of all the desired manipulations. A device for manipulating a target component in a manipulation tube, comprising: a manipulation tube comprising a tube having an optionally-closeable open end for supplying a sample containing a target component at one end and a closed end at the other end, and a manipulation medium accommodated in the tube and having a gel layer and an aqueous liquid layer multilayered in a longitudinal direction of the tube; magnetic particles that should transport the target component; and magnetic field applying means capable of applying a magnetic field to the manipulation tube to move the magnetic particles in the longitudinal direction of the tube. 1. A manipulation tube for manipulating a target component , comprising:a tube having an optionally-closeable open end for supplying a sample containing a target component at one end and a closed end at the other end;a manipulation medium accommodated in the tube and having a gel layer and an aqueous liquid layer alternately layered in a longitudinal direction of the tube; andmagnetic particles that should capture and transport the target component,the magnetic particles being moved in the longitudinal direction of the manipulation tube through the gel layer in a gel state by applied magnetic field.2. The manipulation tube according to claim 1 , wherein the tube has an approximate inner diameter of 0.1 mm to 5 mm.312-. (canceled)13. The manipulation tube according to claim 1 , wherein the magnetic particles have an ability to bind to or adsorb to nucleic acid as the target component claim 1 , and the manipulation medium includes an aqueous liquid layer composed of a liquid that liberates the nucleic acid to bind or adsorb the nucleic acid to the magnetic particles and/or an aqueous liquid layer composed of a liquid for washing ...

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Номер записи: 80
15-03-2018 дата публикации

PROCESS AND SYSTEM FOR PRODUCING PULP, ENERGY, AND BIODERIVATIVES FROM PLANT-BASED AND RECYCLED MATERIALS

Номер: US20180071706A1
Автор: Barla Florin, Bobe Iulian, Kostenyuk Igor, Kumar Sandeep, Majeranowski Peter
Принадлежит:

The presently disclosed subject matter relates to an industrial system for processing various plant materials to produce marketable materials. Particularly, the system integrates subcritical water extraction technology and includes a pre-processing module and a two-stage extractor (processing module) with constant control of temperature, pressure, and/or residence time. In some embodiments, the final product of the disclosed system can include feedstock constituents for biofuel production (sugars and/or oil), biochar, raw materials for various industries (such as pulp for manufacturing paper or cellulose for use in various industries). The disclosed system can be modular or non-modular, stationary or mobile, and can include prefabricated elements with programmed automatic or manual operation so that it can be easily moved and/or assembled on site. 1. A system comprising:a pre-processing portion having a mechanical processor/material handler for extraction of water soluble fermentable carbohydrates and preparation of material for further extraction; a first operating condition at a first pressure and a first temperature at a constant level that is held for a first defined period of time to break down carbohydrates of a first chain strength; and', 'a second operating condition at a second pressure and a second temperature at a constant level that is held for a defined second period of time to break down lignin and the remaining oligo-carbohydrates of a second chain strength and fatty acids, or', 'both the first operating condition and the second operating condition;, 'an extractor portion comprising a reactor or a reactor assembly to which biomass and subcritical water is supplied, the reactor assembly havingwherein the system is repeatable until the recovery rate of the fermentable carbohydrates, fatty acid, or both reaches a desired yield.2. The system according to claim 1 , wherein the mechanical processor/material handler of the pre-processing portion includes a ...

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Номер записи: 81
05-03-2020 дата публикации

PNEUMATIC SYSTEM HAVING NOISE REDUCTION FEATURES FOR A MEDICAL FLUID MACHINE

Номер: US20200069856A1
Автор: Chapman Paul R., Childers Robert W., Hecht Gideon, Wellings Anders
Принадлежит:

A pneumatic system for a medical fluid machine operating a medical fluid cassette, the pneumatic system including an interface for supplying positive pneumatic pressure and negative pneumatic pressure to the medical fluid cassette; a source of positive pneumatic pressure; a source of negative pneumatic pressure; and a pneumatic pump including a first head and a second head, wherein the first head is dedicated to supplying positive pneumatic pressure to the positive pneumatic pressure source and the second head is dedicated to supplying negative pneumatic pressure to the negative pneumatic pressure source. 1. A pneumatic system for a medical fluid machine operating a medical fluid cassette , the pneumatic system comprising:an interface for supplying positive pneumatic pressure and negative pneumatic pressure to the medical fluid cassette;a source of positive pneumatic pressure;a source of negative pneumatic pressure; anda pneumatic pump including a first head and a second head, wherein the first head is dedicated to supplying positive pneumatic pressure to the positive pneumatic pressure source and the second head is dedicated to supplying negative pneumatic pressure to the negative pneumatic pressure source.2. The pneumatic system of claim 1 , which is configured to supply positive pneumatic pressure from the first head to the positive pneumatic pressure source and negative pressure from the second head to the negative pneumatic pressure source simultaneously.3. The pneumatic system of claim 1 , which includes a valve manifold assembly communicating pneumatically between the sources of positive and negative pneumatic pressure and the medical fluid cassette interface.4. The pneumatic system of claim 3 , wherein at least one of the positive pneumatic pressure source claim 3 , the negative pneumatic pressure source claim 3 , or the pneumatic pump is mounted to the valve manifold assembly.5. The pneumatic system of claim 1 , wherein the interface includes a machine ...

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Номер записи: 82
05-03-2020 дата публикации

CELL LINE, SYSTEM AND METHOD FOR OPTICAL-BASED SCREENING OF ION-CHANNEL MODULATORS

Номер: US20200072817A1
Автор: Deisseroth Karl, Gradinaru Viviana, Schneider M. Bret, Zhang Feng
Принадлежит:

A variety of applications, systems, methods and constructs are implemented for use in connection with screening of ion-channel modulators. Consistent with one such system, drug candidates are screened to identify their effects on cell membrane ion channels and pumps. The system includes screening cells having light responsive membrane ion switches, voltage-gated ion switches and fluorescence producing voltage sensors. A chemical delivery device introduces the drug candidates to be screened. An optical delivery device activates the light responsive ion switches. An optical sensor monitors fluorescence produced by the voltage sensors. A processor processes data received from the optical sensor. A memory stores the data received from the optical sensor. 1. A modified cell line derived from parental cell line 293T , wherein the modified cell line comprises:voltage-gated ion channels; andlight-responsive ion switches that mediate depolarization from activation of the voltage-responsive ion channels.2. The cell line of claim 1 , wherein the ion switches are ChR2 ion channels.3. The cell line of claim 1 , wherein the ion switches are NpHR ion pumps.4. The cell line of claim 1 , wherein the voltage-gated ion channels are Ca2+ channels.5. The cell line of claim 1 , wherein the modified cell line further possesses the properties of genetically-encoded ion indicators.6. The cell line of claim 5 , wherein the genetically-encoded ion indicators are calcium indicators7. A system for screening drug candidates to identify their effects on cell membrane ion channels and pumps claim 5 , comprising:screening cells having light responsive membrane ion switches, voltage-gated ion switches and fluorescence producing voltage sensors;a chemical delivery device for introducing the drug candidates to be screened;an optical delivery device to activate the light responsive ion switches;an optical sensor to monitor fluorescence produced by the voltage sensors;a processor to process data ...

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Номер записи: 83
14-03-2019 дата публикации

Biopolymer synthesis system and method

Номер: US20190076814A1
Автор: Fabian Gerlinghaus, II John Andrew Walker, Paul Dabrowski, Reed Kelso
Принадлежит: Synthego Corp

The present invention provides improved automated systems and methods for synthesis of biopolymers including DNA and RNA. The automated systems and methods represent a number of improvements over existing systems for multiplex synthesis of biopolymers in a combinatorial fashion.

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Номер записи: 84
26-03-2015 дата публикации

Multi-stage Process for Forming Polyarylene Sulfides

Номер: US20150087776A1
Автор: Chiong Hendrich, Feord Damian, Grayson Jacob, Haubs Michael, Leonard Stanley, Nekkanti Venkata
Принадлежит:

A multi-stage process and system for formation of a polyarylene sulfide is described. The multi-stage process can include at least three separate formation stages that can take place in three different reactors. The first stage of the formation process can include reaction of an alkali metal sulfide with an organic amide solvent to form a complex including a hydrolysis product of the solvent and an alkali metal hydrogen sulfide. The second stage of the formation process can include reaction of the complex formed in the first stage with a dihaloaromatic monomer to form a prepolymer, and the third stage can include further polymerization of the prepolymer with additional monomers to form the final product. 1. A multi-stage method for forming a polyarylene sulfide comprising:reacting a complex with a first dihaloaromatic monomer to form a polyarylene sulfide prepolymer, the complex including an alkali metal organic amine carboxylic acid salt and an alkali metal hydrogen sulfide;thereafter, carrying out a second polymerization reaction in which the polyarylene sulfide prepolymer is reacted in the organic amide solvent to form the polyarylene sulfide, wherein the molar ratio of any water present in the second polymerization reaction to the sulfur-containing monomer of the second polymerization reaction is less than about 0.2.2. The method of claim 1 , wherein the reaction to form the prepolymer takes place in a first reactor and the second polymerization reaction to form the polyarylene sulfide takes place in a second reactor.3. The method of claim 1 , further comprising reacting a second organic amide solvent claim 1 , an alkali metal sulfide claim 1 , and water to form the complex including the alkali metal organic amine carboxylic acid salt and the alkali metal hydrogen sulfide.4. The method of claim 3 , wherein the formation of the complex takes place in a third reactor.5. The method of claim 3 , wherein the reaction to form the prepolymer take place in a first ...

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Номер записи: 85
26-03-2015 дата публикации

SCRUBBING PROCESS FOR POLYARYLENE SULFIDE FORMATION

Номер: US20150087778A1
Автор: Chiong Hendrich Alvarez
Принадлежит:

A method and system for formation of a polyarylene sulfide is described. The method includes a first stage in which a complex is formed in a reactor. The complex includes the hydrolysis product of an organic amide solvent and an alkali metal hydrosulfide. The complex formation reaction also forms hydrogen sulfide as a by-product. The method also includes treating a fluid stream that is pulled off of the reactor. The treatment includes scrubbing the fluid stream to recover hydrogen sulfide from the stream and return the hydrogen sulfide to the reactor. The recovery and recycle of the hydrogen sulfide can prevent loss of sulfur from a polyarylene sulfide formation process. 1. A method for forming a polyarylene sulfide comprising:reacting a first organic amide solvent with an alkali metal sulfide in the presence of water in a first reactor to form a complex that includes the hydrolysis product of the first organic amide solvent and an alkali metal hydrosulfide, the reaction producing a hydrogen sulfide by-product, the alkali metal sulfide including a molar amount of sulfur;scrubbing a fluid stream that includes the hydrogen sulfide by-product with a scrubbing mixture to form a sulfur-containing mixture, the scrubbing mixture including a second organic amide solvent and an alkali metal hydroxide;charging the sulfur-containing mixture to the first reactor;reacting the complex with a first dihaloaromatic monomer to form a polyarylene sulfide prepolymer; andreacting the polyarylene sulfide prepolymer with a second dihaloaromatic monomer and a sulfur-containing monomer in a third organic amide solvent to form the polyarylene sulfide.2. The method of claim 1 , wherein the amount of the alkali metal hydroxide of the scrubbing mixture is from about 0.1 mole % with respect to the amount of sulfur to about 5 mole % with respect to the amount of the sulfur.3. The method of claim 1 , wherein the amount of the second organic amide solvent of the scrubbing mixture is from about 0.1 ...

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Номер записи: 86
12-03-2020 дата публикации

AUTOMATED COLLECTION OF A SPECIFIED NUMBER OF CELLS

Номер: US20200080046A1
Автор: Allbritton Nancy L., Armistead Paul Michael, Attayek Peter Joseph, Gebhart Steven C., McClellan Robert W., Trotta Nicholas C.
Принадлежит:

Embodiments of the disclosed subject matter provide an automated method and system to isolate and collect cells using computerized analysis of images of cells and their surroundings obtained from a digital imaging device or system. Embodiments of the disclosed subject matter make use of a “microwell array,” which can comprise a formed, elastomeric grid of indentations or “wells.” Many, most, or all of the wells in a microwell array can contain a releasable, microfabricated element, which can be referred to as a “raft.” Embodiments of the disclosed subject matter provide a system and method for cell collection that includes computerized identification and collection of rafts with isolated single cells or a specific group or groups of cells, eliminating the need for continuous human identification and selection. 1. An automated , computer-controlled method for monitoring and collecting selected cells , the method comprising: an imaging device configured for obtaining the images of the microwell array;', 'an actuator configured for releasing the cell rafts from the microwell array;', 'a mechanical cell raft collector; and', obtaining the images of the microwell array using the imaging device;', 'identifying, by analyzing the images of the microwell array, the at least one selected cell raft that contains a cell undergoing a cellular process, storing a location of the selected cell raft on the microwell array, and assigning the selected cell raft to a mapped location of a collection plate;', 'controlling the actuator to release the selected cell raft from the microwell array; and', 'controlling the mechanical cell raft collector to collect the selected cell raft after release from the microwell array and controlling the mechanical cell raft collector to deposit the selected cell raft at the mapped location of the collection plate;, 'a computer system comprising at least one processor and memory, the computer system configured for], 'i) using a system, imaging a ...

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Номер записи: 87
25-03-2021 дата публикации

MICROWAVE ENHANCEMENT OF CHEMICAL REACTIONS

Номер: US20210086158A1
Автор: Boshoff Jan H., Tranquilla James M.
Принадлежит:

Gas streams may be effectively processed using microwave energy in such a way as to significantly reduce processing cost and plant complexity. In the first instance, microwave energy is used to generate a self-catalytic, non-equilibrium plasma, resulting in essentially complete gas reaction at industrial scales of operation. In the second instance, microwave energy is used in combination with conventional catalyst materials to significantly enhance their performance by enabling operation at reduced gas temperatures. In this second instance, the microwave energy may be used either to generate a non-equilibrium plasma or to selectively and directly heat the catalyst material. 1. A system for processing gaseous materials through the use of microwave non-equilibrium plasmas , said system comprisinga) a microwave source connected to a waveguide,b) a means of coupling said microwave energy from the waveguide to a vessel acting as a gas containment reactor vessel in which the plasma is generated and maintained,c) a first means of directing reagent gas into the said reactor vessel by means of supersonic nozzle gas expansion,d) a second means of directing reagent gas tangentially into the said reactor vessel in such a way as to generate a vortex flow which first is directed counter to the supersonic flow direction, is reflected from the top of the said reactor vessel and thereafter is directed in the same direction as the supersonic flow, ande) a means of allowing the post-plasma gas products stream pressure to be adjusted suitably for further processing or discharge.2. The system according to in which the gas containment reactor vessel is constructed of a microwave-transparent material and is located within the said waveguide.3. The system according to in which the gas containment reactor vessel is a metallic cavity which is coupled to the said waveguide by means of an aperture.4. The system according to in which the gas containment reactor vessel is a metallic cavity which ...

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Номер записи: 88
25-03-2021 дата публикации

FORMATION OF ARRAY OF MEMBRANES AND APPARATUS THEREFOR

Номер: US20210086160A1
Автор: Bahamon Pedro Miguel Ortiz, Brown Clive Gavin, Heron Andrew John, Hyde Jason Robert, Mackett Paul Raymond
Принадлежит: Oxford Nanopore Technologies Ltd.

An array of membranes comprising amphipathic molecules is formed using an apparatus comprising a support defining an array of compartments. Volumes comprising polar medium are provided within respective compartments and a layer comprising apolar medium is provided extending across the openings with the volumes. Polar medium is flowed across the support to displace apolar medium and form a layer in contact with the volumes, forming membranes comprising amphipathic molecules at the interfaces. In one construction of the apparatus, the support that comprises partitions which comprise inner portions and outer portions. The inner portions define inner recesses without gaps therebetween that are capable of constraining the volumes comprising polar medium contained in neighbouring inner recesses from contacting each other. The outer portions extend outwardly from the inner portions and have gaps allowing the flow of an apolar medium across the substrate. 162-. (canceled)63. A method of forming an array of membranes comprising amphipathic molecules , the method comprising:{'b': 1', '3', '6', '4', '6', '20', '21', '20', '22', '2', '22', '21', '20', '4', '4, 'providing an apparatus () comprising a support () that comprises partitions () defining an array of compartments (), the partitions () comprising inner portions () and outer portions (), the inner portions () defining inner recesses () without gaps therebetween that are capable of constraining volumes () comprising polar medium that may be contained in neighboring inner recesses () from contacting each other, and the outer portions () extending outwardly from the inner portions () and having gaps allowing the flow of an apolar medium between the compartments (), whereby the compartments () have openings through which polar medium may be introduced;'}{'b': 3', '2', '4', '2', '2', '4', '3', '2, 'disposing polar medium and apolar medium onto the support () to provide volumes () comprising polar medium within respective ...

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Номер записи: 89
29-03-2018 дата публикации

Flow Cell Array and Uses Thereof

Номер: US20180085727A1
Автор: Colgan Evan, Lvov Alexey Y., Polonsky Stanislav
Принадлежит:

Apparatus, computer program products, and methods for using a flow cell array are provided herein. A computer program product includes a computer readable storage medium having program instructions embodied therewith, the program instructions executable by a device to cause the device to determine placement of one or more reaction sites on a first component; provide a material for the reaction sites in one or more surface channels of the first component; connect the first component to a second component to form an array, wherein the surface channels of the first component connect the reaction sites with one or more vias, and wherein the second component comprises the vias connected to multiple sub-surface channels; and align the surface channels of the first component with the vias of the second component to form a connection between the first component and the second component. 1. A computer program product comprising a computer readable storage medium having program instructions embodied therewith , the program instructions executable by a device to cause the device to:determine placement of one or more reaction sites on a first component;provide a material for the one or more reaction sites in one or more surface channels of the first component;connect the first component to a second component to form an array, wherein the one or more surface channels of the first component connect the one or more reaction sites with one or more vias, and wherein the second component comprises the one or more vias connected to multiple sub-surface channels; andalign the one or more surface channels of the first component with the one or more vias of the second component to form a connection between the first component and the second component.2. The computer program product of claim 1 , wherein said determining is based on use of a Gauss number if the number of the one or more reaction sites represents a sum of two integer squares.3. The computer program product of claim 1 , ...

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Номер записи: 90
29-03-2018 дата публикации

Flow Cell Array and Uses Thereof

Номер: US20180085728A1
Автор: Colgan Evan, Lvov Alexey Y., Polonsky Stanislav
Принадлежит:

Systems and methods for using a flow cell array are provided herein. A system includes at least one processor coupled to a memory and configured for determining placement of one or more reaction sites on a first component; providing a material for the one or more reaction sites in one or more surface channels of the first component; connecting the first component to a second component to form an array, wherein the one or more surface channels of the first component connect the one or more reaction sites with one or more vias, and wherein the second component comprises the one or more vias connected to multiple sub-surface channels; and aligning the one or more surface channels of the first component with the one or more vias of the second component to form a connection between the first component and the second component. 1. A system comprising:a memory; and determining placement of one or more reaction sites on a first component;', 'providing a material for the one or more reaction sites in one or more surface channels of the first component;', 'connecting the first component to a second component to form an array, wherein the one or more surface channels of the first component connect the one or more reaction sites with one or more vias, and wherein the second component comprises the one or more vias connected to multiple sub-surface channels; and', 'aligning the one or more surface channels of the first component with the one or more vias of the second component to form a connection between the first component and the second component., 'at least one processor coupled to the memory and configured for2. The system of claim 1 , wherein said determining is based on use of a Gauss number if the number of the one or more reaction sites represents a sum of two integer squares.3. The system of claim 1 , wherein said determining is based on use of numerical approximation.4. The system of claim 1 , wherein the at least one processor is further configured for:independently ...

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Номер записи: 91
31-03-2016 дата публикации

DE NOVO SYNTHESIZED GENE LIBRARIES

Номер: US20160089651A1
Автор: Banyai William, Chen Siyuan, FERNANDEZ Andres, Indermuhle Pierre, Peck Bill James
Принадлежит:

De novo synthesized large libraries of nucleic acids are provided herein with low error rates. Further, devices for the manufacturing of high-quality building blocks, such as oligonucleotides, are described herein. Longer nucleic acids can be synthesized in parallel using microfluidic assemblies. Further, methods herein allow for the fast construction of large libraries of long, high-quality genes. Devices for the manufacturing of large libraries of long and high-quality nucleic acids are further described herein. 120.-. (canceled)21. A device for synthesizing oligonucleic acids of predetermined sequence , the device comprising:a structure having a surface;at least two wells located on the surface, wherein each of the at least two wells has a cross-section of 0.8 mm to 2 mm; and50 to 500 microchannels located at a base of each of the at least two wells, wherein each of the 50 to 500 microchannels has a cross-section of 1 um to 100 um.22. The device of claim 21 , comprising a first coating layer claim 21 , wherein the first coating layer is located on the 50 to 500 microchannels and comprises a moiety bound to the surface claim 21 , wherein the moiety bound to the surface comprises a reactive group that binds to a nucleoside phosphoramidite.23. The device of claim 22 , wherein the moiety bound to the surface is a silane.24. The device of claim 23 , wherein the silane is 11-acetoxyundecyltriethoxysilane claim 23 , n-decyltriethoxysilane claim 23 , (3-aminopropyl)trimethoxysilane claim 23 , (3-aminopropyl)triethoxysilane claim 23 , or N-(3-triethoxysilylpropyl)-4-hydroxybutyramide.25. The device of claim 23 , wherein the silane is an aminosilane.26. The device of claim 21 , comprising a second coating layer claim 21 , wherein the second coating layer is located on the surface and comprises a moiety that binds to the surface of the structure and lacks a reactive group that binds to a nucleoside phosphoramidite.27. The device of claim 26 , wherein the moiety that binds ...

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Номер записи: 92
21-03-2019 дата публикации

Apparatus, System, And Method Using Immiscible-Fluid-Discrete-Volumes

Номер: US20190085387A1
Автор: Cox David M., Lee Linda G., Ma Congcong, Oldham Mark F., REEL Richard T., SCHROEDER Benjamin G., SORENSON Jon M., WIYATNO Willy, WOO Sam L.
Принадлежит:

Various embodiments of the teachings relate to a system or method for sample preparation or analysis in biochemical or molecular biology procedures. The sample preparation can involve small volume processed in discrete portions or segments or slugs, herein referred to as discrete volumes. A molecular biology procedure can be nucleic acid analysis. Nucleic acid analysis can be an integrated DNA amplification/DNA sequencing procedure. 1. A method comprising:amplifying a nucleic acid in at least one conduit to form an amplicon, the at least one conduit comprising an inner wall;attaching the amplicon to the inner wall to form an attached amplicon; anddetecting the attached amplicon or an attached derivative thereof, in the at least one conduit.2. A method comprising:sequentially contacting an aqueous sample fluid in a conduit with a non-aqueous spacing fluid that is immiscible with the aqueous sample, to form a plurality of discrete volumes of the aqueous sample fluid separated from one another by the non-aqueous spacing fluid, the aqueous sample fluid comprising a plurality of target nucleic acid sequences, wherein at least one of the discrete volumes contains at least one target nucleic acid sequence;amplifying the at least one target nucleic acid in the conduit to form an amplicon; andsubjecting the amplicon to a nucleic acid sequencing reaction in the conduit.3. The method of claim 2 , wherein subjecting the nucleic acid sequence to a sequencing reaction forms a detectable product claim 2 , and the method further comprises detecting the detectable product.4. The method of claim 3 , wherein the detectable product is detected inside the conduit.5. The method of claim 3 , wherein the detectable product is detected with a flow cell.6. The method of claim 2 , wherein the sequencing reaction comprises a Sanger sequencing reaction.7. The method of claim 2 , further comprising dividing the at least on nucleic acid containing discrete volume into two or more portions before ...

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Номер записи: 93
02-04-2015 дата публикации

Reagent Bead Inserter

Номер: US20150094238A1
Автор: Bunce Adrian, Fusellier Andrew
Принадлежит: Dynex Technologies, Inc.

A system for inserting reagent or macrobeads into bores of a sample plate is disclosed comprising an insertion device arranged to retain one or more reagent or macrobeads by means of a vacuum whilst inserting the reagent or macrobeads into bores of the sample plate. 1. A system for inserting reagent or macrobeads into one or more bores of a sample plate comprising:an insertion device comprising one or more push or insertion rods;a cartridge comprising, in use, a plurality of reagent or macrobeads; anda sample plate having an upper surface and a lower surface and one or more bores extending from said upper surface through to said lower surface, wherein said cartridge is located, in use, adjacent and/or below said sample plate; andwherein said insertion device is arranged and adapted:(i) to cause said one or more push or insertion rods to enter said cartridge so that each push or insertion rod collects and retains a reagent or macrobead by means of a vacuum, suction or a reduced pressure region; and then(ii) to cause each of said one or more push or insertion rods to exit said cartridge and insert each said reagent or macrobead into a bore of said sample plate via the lower surface of said sample plate, so that each said reagent or macrobead forms a fluid tight circumferential seal with said bore.2. (canceled)3. A system as claimed in claim 1 , wherein said sample plate is arranged so that claim 1 , in use claim 1 , an analyte or other sample when dispensed into said sample plate via said upper surface of said sample plate makes fluid contact with an upper portion of said reagent or macrobead which has not been in direct contact with said one or more push or insertion rods.4. A system as claimed in claim 3 , wherein said sample plate is arranged so that a lower portion of said reagent or macrobead which has been in direct contact with said one or more push or insertion rods does not make fluid contact with said analyte or other sample when said analyte or other sample ...

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Номер записи: 94
05-05-2022 дата публикации

System and method for patterning flow cell substrates

Номер: US20220134333A1
Автор: Dajun Yuan, Randall Smith, Steven MODIANO
Принадлежит: Illumina Inc

A method for patterning flow cell substrates using photo-initiated chemical reactions that includes fabricating a planar waveguide flow cell by forming a layer of light coupling gratings on a glass substrate layer; depositing a core layer on the layer of light coupling gratings; depositing a cladding layer on the core layer; and forming nanowells in the cladding layer; silanizing the cladding layer; coating the silanized cladding layer and nanowells with a first group of reactants; introducing a second group of reactants into the nanowells, wherein the second group of reactants includes a target reactant and a light-sensitive photoinitiator system; coupling a light source to the light coupling gratings and directing light internally within the planar waveguide flow cell for photo-initiating a chemical reaction between the first and second groups of reactants, wherein the photo-initiated chemical reaction covalently binds the target reactant to only the bottom portion of each nanowell.

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Номер записи: 95
07-04-2016 дата публикации

DE NOVO SYNTHESIZED GENE LIBRARIES

Номер: US20160096160A1
Автор: Banyai William, Chen Siyuan, FERNANDEZ Andres, Indermuhle Pierre, Peck Bill James
Принадлежит:

De novo synthesized large libraries of nucleic acids are provided herein with low error rates. Further, devices for the manufacturing of high-quality building blocks, such as oligonucleotides, are described herein. Longer nucleic acids can be synthesized in parallel using microfluidic assemblies. Further, methods herein allow for the fast construction of large libraries of long, high-quality genes. Devices for the manufacturing of large libraries of long and high-quality nucleic acids are further described herein. 120.-. (canceled)21. A device for nucleic acid assembly , comprising:a silicon dioxide structure comprising a plurality of wells, wherein each well of the plurality of wells has height from 0.1 mm to 0.6 mm, and wherein each well of the plurality of wells has a cross-section from 0.8 to 2.0 mm;an inner region of each well of the plurality of wells, wherein the inner region comprises a high surface energy coating;an outer region of each well of the plurality of wells, wherein the outer region comprises a low surface energy coating, and wherein a difference in water contact angle between the inner region and the outer region is at least 10 degrees as measured on a planar surface.22. The device of claim 21 , wherein each well of the plurality of wells comprises an aqueous liquid claim 21 , wherein the aqueous liquid has a surface tension from 12 to 80 millinewtons per meter.23. The device of claim 22 , wherein the aqueous liquid is water.24. The device of claim 22 , wherein the aqueous liquid comprises a DNA polymerase and dNTPs.25. The device of claim 21 , wherein the difference in water contact angle between the inner region and outer region is at least 20 degrees as measured on a planar surface.26. The device of claim 21 , wherein the difference in water contact angle between the inner region and outer region is at least 75 degrees as measured on a planar surface.27. The device of claim 21 , wherein each well of the plurality of wells has an interior ...

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Номер записи: 96
05-04-2018 дата публикации

Flow Cell Array and Uses Thereof

Номер: US20180093243A1
Автор: Colgan Evan, Lvov Alexey Y., Polonsky Stanislav
Принадлежит:

Systems, computer program products, and methods for using a flow cell array are provided herein. A computer program product includes a computer readable storage medium having program instructions embodied therewith, the program instructions executable by a device to cause the device to determine placement of multiple reaction site openings, wherein each reaction site opening is connected to a first sub-surface channel; connect the first sub-surface channel to two or more additional sub-surface channels by multiple vias; and provide a material for multiple reaction sites, wherein an overlap of the multiple reaction site openings and the material delineate the multiple reaction sites. 1. A computer program product comprising a computer readable storage medium having program instructions embodied therewith , the program instructions executable by a device to cause the device to:determine placement of multiple reaction site openings, wherein each reaction site opening is connected to a first sub-surface channel;connect the first sub-surface channel to two or more additional sub-surface channels by multiple vias; andprovide a material for multiple reaction sites, wherein an overlap of the multiple reaction site openings and the material delineate the multiple reaction sites.2. The computer program product of claim 1 , wherein the program instructions executable by a computing device further cause the computing device to:incorporate a cover to seal the multiple reaction site openings.3. The computer program product of claim 2 , wherein said incorporating comprises temporarily securing the cover.4. The computer program product of claim 2 , wherein said incorporating comprises permanently securing the cover.5. The computer program product of claim 1 , wherein said determining is based on use of a Gauss number if the number of the multiple reaction site openings represents a sum of two integer squares.6. The computer program product of claim 1 , wherein said determining is ...

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Номер записи: 97
05-04-2018 дата публикации

Flow Cell Array and Uses Thereof

Номер: US20180093244A1
Автор: Alexey Y. Lvov, Evan Colgan, Stanislav Polonsky
Принадлежит: International Business Machines Corp

Systems, computer program products, and methods for using a flow cell array are provided herein. A system includes at least one processor coupled to a memory and configured for determining placement of multiple reaction site openings, wherein each reaction site opening is connected to a first sub-surface channel; connecting the first sub-surface channel to two or more additional sub-surface channels by multiple vias; and providing a material for multiple reaction sites, wherein an overlap of the multiple reaction site openings and the material delineate the multiple reaction sites.

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Номер записи: 98
05-04-2018 дата публикации

Flow Cell Array and Uses Thereof

Номер: US20180093245A1
Автор: Colgan Evan, Lvov Alexey Y., Polonsky Stanislav
Принадлежит:

Systems, computer program products, and methods for using a flow cell array are provided herein. A computer program product includes a computer readable storage medium having program instructions embodied therewith, the program instructions executable by a device to cause the device to deliver multiple items of chemical matter independently to multiple reaction sites of a flow cell array across multiple distinct instances of time; image multiple parallel chemical reactions at the multiple reaction sites of the flow cell array; and record an emission from each of the multiple chemical reactions site. 1. A computer program product comprising a computer readable storage medium having program instructions embodied therewith , the program instructions executable by a device to cause the device to:deliver multiple items of chemical matter independently to multiple reaction sites of a flow cell array across multiple distinct instances of time;image multiple parallel chemical reactions at the multiple reaction sites of the flow cell array; andrecord an emission from each of the multiple chemical reactions site.2. The computer program product of claim 1 , wherein said multiple distinct instances of time comprise multiple non-overlapping instances of time determined based on at least one of the multiple parallel chemical reactions and the multiple items of chemical matter. Embodiments of the invention generally relate to information technology, and, more particularly, to biological sequencing.Existing deoxyribonucleic acid (DNA) sequencing techniques, such as sequencing-by-synthesis (SBS), sequencing-by-ligation, and pyro-sequencing, use imaging of parallel cyclical chemical reactions. For example, reversible dye-terminators (RDTs) add one fluorescently-labeled nucleotide to a template (single-stranded DNA, for example) per cycle and determine the type of incorporated nucleotide based on the color of the fluorescent label. Such reactions require changing chemicals at every ...

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Номер записи: 99
05-04-2018 дата публикации

Flow Cell Array and Uses Thereof

Номер: US20180093246A1
Автор: Colgan Evan, Lvov Alexey Y., Polonsky Stanislav
Принадлежит:

Systems, computer program products, and methods for using a flow cell array are provided herein. A system includes at least one processor coupled to a memory and configured for delivering multiple items of chemical matter independently to multiple reaction sites of a flow cell array across multiple distinct instances of time; imaging multiple parallel chemical reactions at the multiple reaction sites of the flow cell array; and recording an emission from each of the multiple chemical reactions site. 1. A system comprising:a memory; and delivering multiple items of chemical matter independently to multiple reaction sites of a flow cell array across multiple distinct instances of time;', 'imaging multiple parallel chemical reactions at the multiple reaction sites of the flow cell array; and', 'recording an emission from each of the multiple chemical reactions site., 'at least one processor coupled to the memory and configured for2. The system of claim 1 , wherein said multiple distinct instances of time comprise multiple non-overlapping instances of time determined based on at least one of the multiple parallel chemical reactions and the multiple items of chemical matter. Embodiments of the invention generally relate to information technology, and, more particularly, to biological sequencing.Existing deoxyribonucleic acid (DNA) sequencing techniques, such as sequencing-by-synthesis (SBS), sequencing-by-ligation, and pyro-sequencing, use imaging of parallel cyclical chemical reactions. For example, reversible dye-terminators (RDTs) add one fluorescently-labeled nucleotide to a template (single-stranded DNA, for example) per cycle and determine the type of incorporated nucleotide based on the color of the fluorescent label. Such reactions require changing chemicals at every cycle and rely on fluidic cells to deliver the chemicals to multiple reaction sites. Typically, each cycle of an RDT chemical reaction includes the steps of detritylation, coupling, capping and ...

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Номер записи: 100