ПОДЛОЖКА ДЛЯ ХРАНЕНИЯ ОБРАЗЦА БИОЛОГИЧЕСКОГО МАТЕРИАЛА И СПОСОБ ЕЁ ИЗГОТОВЛЕНИЯ

Изобретение относится к подложке для хранения образцов биологического материала. Описана подложка (1) для хранения образцов биологического материала, где указанная подложка (1) содержит по меньшей мере: первую часть (3) абсорбирующей матрицы (2), предназначенную для хранения образца биологического материала; и вторую часть (4), отличную от первой части (3) и предназначенную для образования очищающей зоны для головки устройства, предназначенного для сбора образца биологического материала с первой части (3), в частности, пуансона, где абсорбирующая матрица изготовлена из материала, основой которого является целлюлоза, и/или бумаги, и/или абсорбирующей пластмассы. Также описаны способ получения подложки, применение подложки, и способ применения подложки. Технический результат: предложена подложка для сбора одного или более образцов биологического материала. 5 н. и 16 з.п. ф-лы, 15 ил.

Запасание жидкости

Настоящая группа изобретений относится к аппарату и способу для направления жидкости в жидкостное устройство и из него, содержащему два или более каналов заливки жидкости, подсоединенных к впуску жидкости жидкостного устройства, клапан управления потоком для каждого канала заливки жидкости для управления потоком между каналом заливки жидкости и впуском жидкости, один или более выпускных каналов, подсоединенных к выпуску жидкости жидкостного устройства, и клапан управления потоком для каждого выпускного канала для управления потоком между выпуском жидкости и соответствующим выпускным каналом. Аппарат для подачи жидкостей на впуск жидкости содержит пластину, выполненную с возможностью вращения вокруг оси вращения, и множество отделений для жидкости, расположенных на пластине, каждое из которых имеет выходное отверстие для жидкости, расположенное на общем радиальном расстоянии от оси вращения и расположенное так, чтобы оно было совмещено с впуском жидкости при вращении пластины вокруг оси ...

ПОДЛОЖКА ДЛЯ ХРАНЕНИЯ ОБРАЗЦА БИОЛОГИЧЕСКОГО МАТЕРИАЛА И СПОСОБ ЕЁ ИЗГОТОВЛЕНИЯ

ИМИТАТОР ТЕПЛОВОГО РАЗДЕЛЕНИЯ ФАЗ

... 1. Имитатор теплового разделения фаз для испытания химических веществ, содержащий:поворотный магазин с кольцевым нагревательным блоком, выполненным из теплопроводного материала и установленным с возможностью поворота на основании, причем поворотный магазин содержитиспытательные ячейки, упорядоченно размещенные по кругу, для приема множества испытательных флаконов,множество нагревательных элементов, расположенных между указанными ячейками, для нагрева теплопроводного материала имножество термопар, расположенных между указанными ячейками, для контроля температуры теплопроводного материала; причемкаждая ячейка имеет канал освещения и вертикальную щель, расположенные снаружи для обеспечения возможности визуального наблюдения или отображения вертикального образца флакона; иисточник освещения, совмещенный с каналом освещения каждой ячейки в ответ на поворот поворотного магазина.2. Имитатор теплового разделения фаз по п.1, в котором каждая ячейка имеет размер, обеспечивающий возможность приема ...

УСТРОЙСТВО ДЛЯ АНАЛИЗА

EINWEGMODUL UND VERFAHREN FÜR EINE TESTANORDNUNG FÜR BIOLOGISCHE PROBEN

Container handling device

A device for handling and removing lids from petri dishes D comprises an elongate handle 10 having a head 11 at one end defining a downwardly-facing mouth for receiving the dish. The handle comprises a pair of arms 10a, 10b provided with respective jaw members 12a, 12b on opposite sides of the mouth at the upper end thereof for engaging a closure C of the dish D. A head member 13 comprises fingers 17a, 17b, on opposite sides of the mouth at the lower end thereof for engaging a base B of the dish D. In use, the device holds the dish D such that the closure C remains fitted to the base B. The device can move the dish D from one position to another without any risk of contamination. Once at the desired position, the handle 10 can be squeezed to open the jaws 12a, 12b to disengage the base B from the device whilst retaining the closure C in-situ. In this manner the base B is released under the influence of gravity downwardly out of the mouth, such that the closure can be separated from the ...

A device for analytical determinations

Microfluidic device for detection of analytes

Devices and methods for detection of biomarkers

Liquid handling

Laboratory in a disk.

An apparatus is described that includes an optical disk, adapted to be read by an optical reader, comprising a first sector having substantially self-contained assay means for localizing an analyte suspected of being in a sample to at least one, predetermined location in the first sector and a second sector containing control means for conducting the assay and analyte location information, with respect to one or more analytes suspected of being in a sample, accessible to the reader, wherein the presence or absence of the analyte at said location is determinable by the reader using the control means and the location information. Depending on the nature of the assay, the disk will include fluid storage means, fluid transfer means, such as one or more capillary ducts, valves, batteries, dialyzers, columns, filters, sources of electric fields, wires or other electrical conductive means such as metallic surface deposits and the like.

Laboratory in a disk

PARTICLE SEDIMENTATION DEVICE AND PROCEDURES FOR WOULD DRIVE THROUGH A PARTICLE SEDIMENTATION

DISK SEPARATOR FOR BLOOD COMPONENTS

SYSTEM FOR THE TRANSPORT OF MICROSCOPIC LIQUID QUANTITIES

ANALYSIS SYSTEM AND PROCEDURE FOR THE ANALYSIS OF A BODY FLUID SAMPLE ON A IN IT ABSTENTION ANALYTEN

ROTOR AND PROCEDURE FOR THE AUTOMATIC PROCESSING OF LIQUIDS FOR LABORATORY AND BIOANALYTIC PURPOSES

GENERAL-PURPOSE REACTION PLATE FOR ANALYSIS, IN PARTICULAR FOR ANALYSIS OF FULL BLOOD OR OF SOLID PARTICLE CONTAINING FLUIDS

PARTICLE SEDIMENTATION DEVICE AND PROCEDURES FOR WOULD DRIVE THROUGH A PARTICLE SEDIMENTATION

PARTICLE SEDIMENTATION DEVICE AND PROCEDURES FOR WOULD DRIVE THROUGH A PARTICLE SEDIMENTATION

PARTICLE SEDIMENTATION DEVICE AND PROCEDURES FOR WOULD DRIVE THROUGH A PARTICLE SEDIMENTATION

PARTICLE SEDIMENTATION DEVICE AND PROCEDURES FOR WOULD DRIVE THROUGH A PARTICLE SEDIMENTATION

PARTICLE SEDIMENTATION DEVICE AND PROCEDURES FOR WOULD DRIVE THROUGH A PARTICLE SEDIMENTATION

PARTICLE SEDIMENTATION DEVICE AND PROCEDURES FOR WOULD DRIVE THROUGH A PARTICLE SEDIMENTATION

PARTICLE SEDIMENTATION DEVICE AND PROCEDURES FOR WOULD DRIVE THROUGH A PARTICLE SEDIMENTATION

PARTICLE SEDIMENTATION DEVICE AND PROCEDURES FOR WOULD DRIVE THROUGH A PARTICLE SEDIMENTATION

PARTICLE SEDIMENTATION DEVICE AND PROCEDURES FOR WOULD DRIVE THROUGH A PARTICLE SEDIMENTATION

A system and method for calibrating a centrifuge

A sample holder for use in a centrifuge, the sample holder being generally planar and comprising: an aperture or recess for releasably retaining a sample storage member including a sample chamber adapted to contain a volume of liquid; a centre point around which the holder will rotate during use; and one or more calibration features, wherein the calibration feature(s) comprise one or more outer edges, which lie on the side of the or each calibration feature which is furthest from the centre point, and the one or more outer edges comprise a series of radially spaced-apart outer edge portions or positions which are spaced at different distances from the centre point as a function of angular position around the centre point.

Microfluidic rotor device

Described herein are various embodiments directed to rotor devices, systems, and kits. Embodiments of rotors disclosed herein may be used to characterize one or more analytes of a fluid. An apparatus may include a first layer being substantially transparent. A second layer may be coupled to the first layer to collectively define a set of wells. The second layer may define a channel, and the second layer may be substantially absorbent to infrared radiation. A third layer may be coupled to the second layer. The third layer may define an opening configured to receive a fluid. The third layer may be substantially transparent. The channel may establish a fluid communication path between the opening and the set of wells.

Microfluidic rotor device

Described herein are various embodiments directed to rotor devices, systems, and kits. Embodiments of rotors disclosed herein may be used to characterize one or more analytes of a fluid. An apparatus may include a first layer being substantially transparent. A second layer may be coupled to the first layer. The second layer may be substantially absorbent to infrared radiation. The second layer and the first layer may collectively define a set of wells. The first layer may define a base for each well of the set of wells. The second layer may define an opening for each well of the set of wells. At least one of the first layer and the second layer may define a sidewall for each well of the set of wells.

Methods, devices, and systems for analyte detection and analysis

Provided are systems and methods for analyte detection and analysis. A system can comprise an open substrate. The open substrate may be configured to rotate or otherwise move. The open substrate can comprise an array of individually addressable locations, with analytes immobilized thereto. The substrate may be spatially indexed to identify nucleic acid molecules from one or more sources, and/or sequences thereof, with the respective one or more sources. A solution comprising a plurality of probes may be directed across the array to couple at least one of the plurality of probes with at least one of the analytes to form a bound probe. A detector can be configured to detect a signal from the bound probe via scanning of the substrate while minimizing temperature fluctuations of the substrate or optical aberrations caused by bubbles.

Devices with optically readable liquid reservoirs

A device includes a lower reservoir surface, an upper reservoir surface, and a reservoir sidewall extending between the upper and lower reservoir surfaces which together define a reservoir. The reservoir is configured to be completely filled by a liquid such that the liquid forms a column contacting the upper reservoir surface, the lower reservoir surface, and the reservoir sidewall, with a meniscus of the liquid being outside of the reservoir. At least one of the upper reservoir surface and the lower reservoir surface is configured to transmit light.

Blood plasma separation device

A device for separating blood plasma from whole blood includes a first reservoir and a second reservoir. The first reservoir is configured to receive a sample of whole blood including red blood cells and includes a collection region and a constricted region. The second reservoir is fluidically connected to the constricted region of the first reservoir, such that, responsive to centrifugal force applied to the device, the sample of whole blood disposed within the first reservoir separates into a first fraction and a second fraction. The first fraction is located in the collection region and includes blood plasma from which substantially all red blood cells have been removed. The second fraction is located in the second reservoir and includes blood plasma and red blood cells that have been removed from the first fraction by the centrifugal force. The constricted region inhibits the second fraction from entering the collection region.

Optical density instrument and systems and methods using the same

Instruments, systems, and methods for measuring optical density of microbiological samples are provided. In particular, optical density instruments providing improved safety, efficiency, comfort, and convenience are provided. Such optical density instruments include a handheld portion and a base station. The optical density instruments may be used in systems and methods for measuring optical density of biological samples.

Analyte test system for determining the concentration of an analyte in a physiological fluid

Identification and quantification of a plurality of biological (micro)organisms or their components

ANALYZING TOOL

System for PCR sample preparation and for PCR set-up

SYSTEM FOR PCR SAMPLE PREPARATION AND FOR PCR SET-UP There is disclosed a system (100) for PCR sample preparation and for PCR set-up, the system comprising a loading device (101) for receiving sample tubes (102) for setting up a PCR, wherein the loading device comprises a first receiving section (200), wherein the first receiving section comprises at least a first receiving and a second receiving opening, wherein the first receiving opening (201) and the second receiving opening (202) are adapted to respectively receive a sample tube, the system further comprising a tube holding device (103) for being attached to the loading device, wherein the tube holding device comprises at least a first receiving opening (105), and wherein the tube holding device is attachable (203) to the loading device such that the receiving opening (105) of the tube holding device is aligned with at least one of the first receiving opening (201) and the second receiving opening (202) of the loading device and simultaneously ...

Method for producing microcarriers

The present invention relates to a method for producing microcarriers comprising the following steps: (a) providing a wafer (6) having a sandwich structure comprising a bottom layer (7), a top layer (8) and a insulating layer(9) located between said bottom and top layers (7, 8), (b) etching away the top layer (8) to delineate lateral walls (12) of bodies (11) of the microcarriers, (c) depositing a first active layer (13)at least on a top surface (14) of the bodies (11), (d) applying a continuous polymer layer (16) over the first active layer(13), (e) etching away the bottom layer (7) and the insulating layer (9), (f) removing the polymer layer (16) to release the microcarriers.

Fluidic devices and systems for sample preparation or autonomous analysis

The present invention relates to fluidic devices for preparing, processing, storing, preserving, and/or analyzing samples. In particular, the devices and related systems and methods allow for preparing and/or analyzing samples (e.g., biospecimen samples) by using one or more of capture regions and/or automated analysis.

Microfluidic device for detection of analytes

A microfluidic device for detection of an analyte in a fluid is described. The microfluidic device comprises a substrate having a first surface defining entrances to one or more chambers defined in the substrate, surfaces of the chambers defining a second surface of the substrate, the first surface being modified for selective targeting and capture of at least one analyte to operably effect a blocking of the entrance to at least one of the chambers, and wherein a response characteristic of the microfluidic device is operably varied by the blocking of the entrance to the at least one of the chambers, thereby providing an indication of the presence of the analyte within the fluid.

Laboratory in a disk

Microfluidic device

Microfluidic device

A disposable cartridge and method for an assay of a biological sample

STRUCTURAL UNITS THAT DEFINE FLUIDIC FUNCTIONS

A microfluidic device that comprises several microchannel structures in which there are an inlet port, an outlet port and therebetween a substructure comprising a fluidic function. The device has an axis of symmetry around which the microchannel structures are arranged as two or more concentric annular zones. for an inlet port and an outlet port of the same microchannel structure the inlet port is typically closer to the axis of symmetry than the outlet port. Each microchannel structure comprises a substructure that can retain liquid while the disc is spun around the axis and/or the inlet ports are positioned separate from the paths waste liquid leaving open waste outlet ports will follow across the surface of the disc when it is spun. For the microchannel structures of an annular zones the corresponding substructures are at essentially at the same radial distance while corresponding substructures in microchannel structures of different annular zones are at different radial distances. The ...

METHOD AND APPARATUS FOR CONDUCTING AN ASSAY

The present invention relates to methods and apparatus for conducting an assay. In particular, the present invention relates to a rotatable platform which can be used for conducting an assay, in particular multi-step assays. The present invention provides a rotatable platform adapted to immobilise a first binding partner in one or more discrete areas on a surface of said platform, or to selectively immobilise a second binding partner in one or more discrete areas on a surface of said platform. The invention also relates to methods, apparatus, a kit and the use of the rotatable platform for conducting an assay. In particular, the invention has been developed primarily for use in sequencing nucleic acid by pyrosequencing, however the invention is not limited to this field.

POINT OF CARE CONCENTRATION ANALYZER

An analyzer system includes a cartridge configured to receive a sample. The cartridge has a plurality of chambers for isolating a target analyte of the sample and collecting a quantity of a first label that is proportional to a quantity of the target analyte in the sample. The system includes an analyzer with a first electromagnetic radiation source a first detector and a controller. The first electromagnetic radiation source is configured to provide electromagnetic radiation to form an interrogation space within a detection chamber of the cartridge. The first detector is configured to detect electromagnetic radiation emitted in the interrogation space by the first label if the first label is present in the interrogation space. The controller is configured to identify the presence of the target analyte in the sample based on electromagnetic radiation detected by the first detector.

CHARACTERIZATION OF REACTION VARIABLES

A microscale method for the characterization of one or more reaction variables that influence the formation or dissociation of an affinity complex comprising a ligand and a binder, which have mutual affinity for each other. The method is characterized in comprising the steps of: (i) providing a microfluidic device comprising a microchannel structures that are under a common flow control, each microchannel structure comprising a reaction microactivity; (ii) performing essentially in parallel an experiment in each of two or more of the plurality of microchannel structures, the experiment in these two or more microchannel structures comprising either a) formation of an immobilized form of the complex and retaining under flow conditions said form within the reaction microactivity, or b) dissociating, preferably under flow condition, an immobilized form of the complex which has been included in the microfluidic device provided in step (i), at least one reaction variable varies or is uncharacterized ...

SAMPLE PROCESSING DEVICE HAVING PROCESS CHAMBERS WITH BYPASS SLOTS

Sample processing devices including process chambers having bypass slots and methods of using the same are disclosed. The bypass slots are formed in the sidewalls of the process chambers and are in fluid communication with distribution channels used to deliver fluid sample materials to the process chambers ...

MICROFLUIDIC DEVICE FOR DETECTION OF ANALYTES

A microfluidic device for detection of an analyte in a fluid is described. The microfluidic device comprises a substrate having a first surface defining entrances to one or more chambers defined in the substrate, surfaces of the chambers defining a second surface of the substrate, the first surface being modified for selective targeting and capture of at least one analyte to operably effect a blocking of the entrance to at least one of the chambers, and wherein a response characteristic of the microfluidic device is operably varied by the blocking of the entrance to the at least one of the chambers, thereby providing an indication of the presence of the analyte within the fluid.

MULTI FUNCTION SPINNING PLATFORM

The present disclosure relates to rotatable platform for use in Lab-on-disc (LoD) applications, and LoD microfluidic device having the rotatable platform and which is suitable for analysis of a fluid sample. The present disclosure further relates to realtime monitoring of cells using a centrifugal microfluidic platform. In particular, the present disclosure relates to a mobile LoD device, which can be used in remote destinations and for point of care applications. One embodiment relates to a method for monitoring microorganisms under the constant supply of nutrients, comprising the step of inoculating the cells in a culture chamber in a rotatable microfluidic platform, rotating the platform such that liquid in a reservoir connected to or located on the platform, the liquid comprising nutrients for the cells, is constantly supplied to the cells in the culture chamber by means of shear / centrifugal force resulting from rotating the platform, and continuously monitoring the cells during rotation ...

MICROFLUIDIC ROTOR DEVICE

Described herein are various embodiments directed to rotor devices, systems, and kits. Embodiments of rotors disclosed herein may be used to characterize one or more analytes of a fluid. An apparatus may include a first layer being substantially transparent. A second layer may be coupled to the first layer to collectively define a set of wells. The second layer may define a channel, and the second layer may be substantially absorbent to infrared radiation. A third layer may be coupled to the second layer. The third layer may define an opening configured to receive a fluid. The third layer may be substantially transparent. The channel may establish a fluid communication path between the opening and the set of wells.

METHODS FOR MANUFACTURING A MICROFLUIDIC ROTOR DEVICE

Described herein are various embodiments directed to rotor devices, methods, and systems. Embodiments of rotors disclosed herein may be used to characterize one or more analytes of a fluid. A manufacturing method may include bonding a first layer and a second layer using two-shot injection molding. The first layer coupled to the second layer collectively defines a set of wells or cuvettes. The first layer is substantially transparent. The second layer defines a channel. The second layer is substantially absorbent to infrared radiation and may comprise some amount of carbon black or of a laser absorbing dye. A third layer is bonded to the second layer using infrared radiation, e.g. using a near-infrared radiation at a wavelength of about 940 nm. The third layer defines an opening configured to receive a fluid and is substantially transparent. The channel establishes a fluid communication path between the opening and the set of wells. By combining injection molding and laser welding techniques ...

ASSAY DEVICE AND METHOD FOR ASSESSING BLOOD CELLS

The present invention relates to an assay device and its use in medical analytics, in particular a method for assessing blood or blood cells.

SAMPLE-TO-ANSWER MICROFLUIDIC SYSTEM AND METHOD INCLUDING VERTICAL MICROFLUIDIC DEVICE AND AUTOMATED ACTUATION MECHANISMS

A sample-to-answer microfluidic system and method including vertical microfluidic device and automated actuation mechanisms, such as, but not limited to, automated mechanical and/or magnetic actuation, is disclosed. In some embodiments, the sample-to-answer microfluidic system includes a vertically oriented microfluidic device and a rotating actuator in relation to the microfluidic device, and wherein the microfluidic device and the rotating actuator are operating in the XZ plane. Additionally, methods of using the sample-to-answer microfluidic system are provided.

SAMPLE PROCESSING DEVICE WITH COMPRESSION SYSTEMS AND METHOD OF USING SAME

Sample processing systems and methods of using those systems for processing sample materials located in sample processing devices are disclosed. The sample processing systems include a rotating base plate on which the sample processing devices are located during operation of the systems. The systems also include a cover and compression structure designed to force a sample processing device towards the base plate. The preferred result is that the sample processing device is forced into contact with a thermal structure on the base plate. The systems and methods of the present invention may include one or more of the following features to enhance thermal coupling between the thermal structure and the sample processing device: a shaped transfer surface, magnetic compression structure, and floating or resiliently mounted thermal structure. The methods may preferably involve deformation of a portion of a sample processing device to conform to a shaped transfer surface.

HIGH DENSITY ELECTROPHORESIS DEVICE AND METHOD

The apparatus comprises a disc-shaped substrate (20) defining a central reservoir (24), a plurality of coplanar electrophoretic channels (22) in fluid communication with, and emanating substantially radially from, the central reservoir (24), and each channel (22) having a proximal end (22a) which is linked to the central reservoir, and a distal end (22b), and preferably for each channel, at least one chamber, and preferably three chambers, linked by a passageway in fluid communication with the distal end of that channel. Preferably, each passageway leads from each chamber in a direction that is initially away from the central reservoir, whereby centrifugation of the substrate about a central axis that is perpendicular to the channels is effective to disperse liquid from the central reservoir into the chambers, channels, and passageways such that any air bubbles in the chambers, channels, and passageways are forced towards the axis of rotation, when such liquid is present in the central ...

DEVICES AND METHODS FOR PROGRAMMABLE MICROSCALE MANIPULATION OF FLUIDS

The present invention is directed generally to devices and methods for controlling fluid flow in meso-scale fluidic components in a programmable manner. Specifically, the present invention is directed to an apparatus and method for placing two microfluidic components in fluid communication at an arbitrary position and time, both of which are externally defined. The inventive apparatus uses electromagnetic radiation to perforate a material layer having selected adsorptive properties. The perforation of the material layer allows the fluid communication between microfluidic components. Other aspects of this invention include an apparatus and method to perform volumetric quantitation of fluids, an apparatus to program arbitrary connections between a set of input capillaries and a set of output capillaries, and a method to transport fluid in centripetal device from a larger to a smaller radius. In addition, the present invention also is directed to a method to determine the radial and polar ...

SYSTEMS AND METHODS FOR CENTRIFUGE SAMPLE HOLDERS

The systems and methods of the invention provide for sample holders (100) for centrifuges that include a channel structure (103) having a sample chan nel (104) and an overflow channel (106). The sample channel (104) and the ov erflow channel (106) are configured such that any excess sample flows into t he overflow channel (106) thereby maintaining a constant sample level in the sample channel (104). In other aspects, the invention provides for centrifu ges (500) comprising sample holders (100) having a plurality of channel stru ctures (103). In still other aspects, the invention provides for methods of using the sample holder (100) and methods for detecting species in a sample using luminescence based measurement techniques.

MICROFLUIDIC DEVICE FOR HIGH-VOLUME PRODUCTION AND PROCESSING OF MONODISPERSE EMULSIONS

A high volume microfluidic system for producing emulsions includes a fluid distribution network to produce uniformly sized emulsions and encapsulates.

DEVICE FOR SEPARATING BUBBLES FROM A FLUID

The present invention relates to a device and a method for separating bubbles from a fluid comprising a chamber through which a fluid can pass. According to the invention the device is characterized in that the inner chamber wall has a geometry that generates within said chamber a continuous flow and at least one area with a discontinuous flow which has a low flow velocity so that bubbles remain at the inner chamber wall in said area and thus are separated from the fluid flowing out from the chamber.

METHOD FOR PRODUCING A MEMBRANE RING OR TEST STRIP RING AND RING MAGAZINE

The invention relates to a method for producing a membrane ring or test strip ring for a diagnostic test device, wherein a) an elongated strip (26) is divided into segments (20) by cuts (24) running transverse to the longitudinal axis of the strip, b) wherein the cuts (24) are made only as far as a residual width of the strip (26), so that a material bridge (38) remains intact between segments (20) adjacent at the cuts (24); c) the strip (26) is closed by joining the ends thereof together into a ring (22), wherein the cut edges of the cut (24) running toward the material bridges (38) each enclose an acute angle (a); d) the ring (22) is placed in a support structure (12) as a membrane ring or test strip ring for the test device.

ROTOR AND METHOD FOR AUTOMATICALLY PROCESSING LIQUIDS FOR LABORATORY AND BIOANALYSIS PURPOSES

... 2053894 9015321 PCTABS00002 A rotor assembly (50) for performing a relatively large number of processing steps upon a sample, such as a whole blood sample, and a diluent, such as water, includes a rotor body (52) for rotation about an axis (R) and including a network of chambers within which various processing steps are performed upon the sample and diluent; and passageways through which the sample and diluent; are transferred. A transfer mechanism (60) is movable through the rotor body by the influence of a magnetic field generated adjacent the transfer mechanism and movable along the rotor body, and the assembly utilizes centrifugal force, a transfer of momentum and capillary action to perform any of a number of processing steps such as separation, aliquoting, transference, washing, reagent addition and mixing of the sample and diluent within the rotor body. The rotor body is particularly suitable for automatic immunoassay analyses.

Coverslip holder

Support pour objets en forme de disques et procédé pour sa fabrication

Transportund �bergabevorrichtung f�r scheibenf�rmige substrates, vacuum treatment plant and procedure for the production of treated substrates.

Die Erfindung betrifft eine Transport- und Übergabevorrichtung für scheibenförmige Substrate, umfassend einen Träger (3) und eine Übernahmeanordnung (15). Beide sind relativ zueinander beweglich. Ein relativ schwerer Substratträger (7) aus magnetisierbarem Material wird durch Abstands-Steuerung vom Permanent-Magneten (17) an der Übernahme-Anordnung (15) von Letzterer übernommen, bzw. von Letzterer an einen Träger (3) rückübergeben. Der gesteuerte Antrieb der Permanent-Magnete (17) in der Übernahme-Anordnung (15) erfolgt mittels pneumatischer Kolben-/Zylinder-Anordnungen (19).

Test cartridge with integrated transfer module

Microfluidic inspection device and method for operating thereof

Specimen measurement device and method for circulating air in reagent storage

A specimen measurement device which suppresses increases in the bulkiness of the device and suppresses increases in the number of components, and a method for circulating air in a reagent storage, areprovided. The specimen measurement device 100 is provided with a measurement unit 10 which uses a reagent stored in a reagent container 200 to measure a specimen; a reagent storage 20 which stores reagent containers 200; a reagent container holder 30 which is arranged in the reagent storage 20 and which holds reagent containers 200; a drive unit 50 which rotationally drives the reagent containerholder 30; and a fin 40 which is driven to rotate coupled with the reagent container holder 30 and which circulates the air in the reagent storage 20.

CHART OF ENUMERATION AND CHARACTERIZATION OF MICRO-ORGANISMS

MICROFLUIDIC DEVICE COMPRISING TWO SUBSTRATES WHICH ARE BONDED WITH EACH OTHER AND A METHOD FOR FABRICATING THE SAME

PURPOSE: A microfluidic device and a method for fabricating the same are provided to improve bonding reliability by suppressing the formation of air bubbles when bonding two substrates using an adhesive. CONSTITUTION: A microfluidic device includes a microflow structure(20), a lower plate protrusion pattern(30), and an adhesive layer. A platform includes an upper and lower plates(12,14) which are bonded with each other. The microflow structure is formed by grooves on the lower plate. The lower plate protrusion pattern includes an outer protrusion of a structure. The outer protrusion of the structure is protruded from the lower substrate to the upper substrate along the outer circumstance of the microflow structure. The adhesive layer is interposed between the lower plate protrusion pattern and upper plate. COPYRIGHT KIPO 2010 ...

MICROFLUIDIC DEVICE AND A SPECIMEN TESTING DEVICE USING THE SAME, CAPABLE OF OMITTING A SEPARATE PRE-PROCESSING PROCESS BY INCLUDING AN ANTICOAGULANT CHAMBER IN THE MICROFLUIDIC DEVICE

PURPOSE: A microfluidic device and a specimen testing device using the same are provided to implement various kinds of tests with using specimen by placing different kinds of plural anticoagulant agents in one platform. CONSTITUTION: A specimen chamber(40) contains specimen. An anticoagulant chamber(50) contains an anticoagulant agent. A channel connects the specimen chamber and the anticoagulant chamber. A valve(70) opens and closes the channel. A specimen testing device includes a valve opening unit and a controlling unit. The controlling unit drives the valve opening unit in order to selectively mix the specimen and the anticoagulant agent. COPYRIGHT KIPO 2011 ...

controle de sequência de fluxo de líquido em dispositivo microfluídico

MULTI-COLOR FLUORESCENT EXCITATION AND DETECTION DEVICE AND NUCLEIC ACID ANALYSIS APPARATUS EMPLOYING SAME

Testing module

MULTI-WELL PLATE WITH ALIGNMENT GROOVES FOR ENCODED MICROPARTICLES

A method and apparatus are provided for aligning optical elements or microbeads, wherein each microbead has an elongated body with a code embedded therein along a longitudinal axis thereof to be read by a code reading device. The microbeads are aligned with a positioning device so the longitudinal axis of the microbeads is positioned in a fixed orientation relative to the code reading device. The microbeads are typically cylindrically shaped glass beads between 25 and 250 microns (µm) in diameter and between 100 and 500 µm long, and have a holographic code embedded in the central region of the bead, which is used to identify it from the rest of the beads in a batch of beads with many different chemical probes. A cross reference is used to determine which probe is attached to which bead, thus allowing the researcher to correlate the chemical content on each bead with the measured fluorescence signal. Because the code consists of a diffraction grating typically disposed along ...

FLOW-THROUGH CELL AND METHOD OF USE

There is disclosed a flow-through cell comprising a substrate defining a channel, having an inlet and an outlet, at least a portion of the substrate being light-permeable to allow particles within at least a portion of the channel between the inlet and the outlet to be optically detected through the substrate, wherein the flow-through cell comprises liquid-permeable particle retaining means located downstream of the at least a portion of the channel where particles can be optically detected, for allowing the flow of a liquid sample through the channel from the inlet to the outlet while retaining particles from the liquid sample whose dimensions exceed threshold dimensions within the channel, where they can be optically detected. The flow- through cell is particularly applicable to the detection of micro-organisms in drinking water.

SLIDE CARTRIDGE AND REAGENT TEST SLIDES FOR USE WITH A CHEMICAL ANALYZER, AND CHEMICAL ANALYZER FOR SAME

A slide cartridge (1) for use with a chemical analyzer includes an upper ring (2) and a lower ring (4) secured together but rotatable with respect to each other. The upper and lower rings define a plurality of reaction chambers (6) between them, which receive dry analyte test slides (8). A gear track formed in the underside of the lower ring engages a pinion gear (28) attached to a stepping motor (30) of the chemical analyzer in order to rotate the slide cartridge. The slide cartridge is rotated under a sample fluid metering device (68), which deposits a sample fluid on the test slides through a plurality of spotter ports (66) formed in the upper ring, and above a reflectometer (22), which performs a colorimetric measurement on the spotted test slides through viewing windows (18) formed in the lower ring of the slide cartridge.

Dual bead assays including optical biodiscs and methods relating thereto

A method for determining whether a target agent is present in a biological sample includes mixing magnetic capture beads, each having at least one transport probe affixed thereto, reporter beads, each bead having at least one signal probe affixed thereto, and a biological sample, under binding conditions which permit formation of a dual bead complex if the target agent is present in the sample, wherein the dual bead complex comprises the reporter bead bound to the target agent which is in turn bound to the magnetic capture bead; isolating the dual bead complex from the mixture by subjecting the mixture to a magnetic field; loading the isolate into an optical disc, the optical disc having an capture layer having affixed thereto, within a capture field, a capture agent that binds to the dual bead complex specifically; and detecting the presence of the dual bead complex in the optical disc, the presence of the dual bead complex indicating that the target agent is present in the sample.

Valve unit and reaction apparatus having the same

Provided are a valve unit and a reaction apparatus having the valve unit. The valve unit includes a phase transition material, which melts and expands upon an application of the electromagnetic waves to the valve filler, and the valve filler is directed into the channel through the connection passage and closes the channel. The valve unit also includes heat generation particles, which are dispersed in the phase transition material and generate heat upon an application of electromagnetic wave energy.

Method for producing microcarriers

The present invention relates to a method for producing microcarriers comprising the following steps: (a) providing a wafer having a sandwich structure comprising a bottom layer, a top layer and an insulating layer located between said bottom and top layers, (b) etching away the top layer to delineate lateral walls of bodies of the microcarriers, (c) depositing a first active layer at least on a top surface of the bodies, (d) applying a continuous polymer layer over the first active layer, (e) etching away the bottom layer and the insulating layer, (f) removing the polymer layer to release the microcarriers.

Non-contact radiant heating and temperature sensing device for a chemical reaction chamber

An apparatus and methods are provided for heating and sensing the temperature of a chemical reaction chamber without direct physical contact between a heating device and the reaction chamber, or between a temperature sensor and the reaction chamber. A plurality of chemical reaction chambers can simultaneously or sequentially be heated independently and monitored separately.

Compact disk based platform for separating and detecting immunomagnetic bead labeled cells

Disclosed is a disk based platform for separating and detecting cells that are labeled with immunomagnetic beads. A disk-like carrier board forms therein at least one flow channel structure, which includes an inner reservoir for receiving a sample fluid, an outer reservoir, and a plurality of micro flow channels arranged between and in fluid communication with the inner and outer reservoirs. When the disk-like carrier board spins, cells labeled with immunomagnetic beads are attracted by a magnetic attraction unit that is arranged above the disk-like carrier board and adjacent to the inner reservoir to thereby remain in the inner reservoir, and cells that are not so labeled are acted upon by a centrifugal force induced by the spinning of the disk-like carrier board to flow with the sample fluid from the inner reservoir through the micro flow channels into the outer reservoir.

Microfluidic system

A dual-side field-emission display has one cathode plate and two anode plates. The cathode plate has two opposite surfaces, and each surface has a cathode conductive layer and an electron emission layer formed thereon. The anode plates are disposed at two opposing sides of the cathode plate. Each of the anode plates has an anode conductive layer and a phosphor layer.

Method and apparatus for controlling fluid movement in a microfluidic system

The invention provides a method for moving a fluid sample within an open channel flow device by centrifugal force and specially adapted apparatus for practicing the method of the invention. The inventive method is a mechanically simple method for moving a fluid in a platform by changing the orientation of a platform relative to the direction of applied forces when the centrifuge rotor is at rest in order to move a fluid sequentially through a plurality of chambers, wherein movement of the fluid is controlled by the location or size of the passages connecting chambers relative to the direction of forces acting on the fluid.

Methods for detecting an analyte using an analyte detection device

The present invention relates to an analyte detection device comprising: a sample chamber for storing a mixture solution of a sample comprising an analyte and a reactant comprising particles; a detection chamber for storing a detection solution; and a channel placed between the sample chamber and the detection chamber to prevent the mixture solution and the detection solution from being mixed with each other, the analyte detection device characterized by detecting the analyte by moving the particles from the sample chamber to the detection chamber using moving means.

MICROFABRICATED INTEGRATED DNA ANALYSIS SYSTEM

Methods and apparatus for genome analysis are provided. A microfabricated structure including a microfluidic distribution channel is configured to distribute microreactor elements having copies of a sequencing template into a plurality of microfabricated thermal cycling chambers. A microreactor element may include a microcarrier element carrying the multiple copies of the sequencing template. The microcarrier element may comprise a microsphere. An autovalve at an exit port of a thermal cycling chamber, an optical scanner, or a timing arrangement may be used to ensure that only one microsphere will flow into one thermal cycling chamber wherein thermal cycling extension fragments are produced. The extension products are captured, purified, and concentrated in an integrated oligonucleotide gel capture chamber. A microfabricated component separation apparatus is used to analyze the purified extension fragments. The microfabricated structure may be used in a process for performing sequencing ...

Characterization of reaction variables

A microscale method for the characterization of one or more reaction variables that influence the formation or dissociation of an affinity complex comprising a ligand and a binder, which have mutual affinity for each other. The method is characterized in comprising the steps of: (i) providing a microfluidic device comprising a microchannel structures that are under a common flow control, each microchannel structure comprising a reaction microactivity; (ii) performing essentially in parallel an experiment in each of two or more of the plurality of microchannel structures, the experiment in these two or more microchannel structures comprising either a) formation of an immobilized form of the complex and retaining under flow conditions said form within the reaction microactivity, or b) dissociating, preferably under flow condition, an immobilized form of the complex which has been included in the microfluidic device provided in step (i), at least one reaction variable varies or is uncharacterized ...

Testing multiple fluid samples with multiple biopolymer arrays

A method of testing multiple fluid samples with multiple biopolymer arrays. A cover is assembled to a contiguous substrate carrying on a first side, multiple arrays each with multiple regions of biopolymers linked to the substrate, such that the cover and the substrate together form a plurality of chambers each containing a biopolymer array and each being accessible through its own port. Multiple fluid samples are introduced into respective chambers through a port of each such that the fluid samples contact respective arrays. A binding pattern of the arrays is observed. An apparatus and kit useful in such methods, are also provided.

MULTI VESSEL RING

A multi vessel ring (suitable for use with thermal cycler and PCR apparatus) comprises a ring body and a plurality of elongate tubes. Each tube has a proximal open end and a distal closed end, each elongate tube being integrally formed with the ring body, and being pivotally connected to the ring body between an initial position in which a longitudinal axis of each tube is generally parallel with an axis of rotation of the ring body, and a final position in which the longitudinal axis of each tube is inclined relative to the axis of rotation of the ring body. The multi vessel ring preferably further comprises a plurality of caps integrally formed with the ring body, and adapted to seal the proximal open end of respective tubes. A further embodiment is directed to an assembly comprising said ring and a carrier disc having a central hub adapted to be mounted to a thermal cycler, a circumferentially outer portion of the carrier disc having a plurality of grooves, each groove being adapted to support one of said tubes when the multi-vessel ring is located on the carrier disc. A yet further embodiment is directed to a capping tool adapted for use with such an assembly, comprising a loading block having a plurality of projections, each projection being insertable within an aperture formed in an underside of the ring body to push one said cap body from an initial position, in which the cap body is generally coplanar with the ring body, to an intermediate position in which the cap body has rotated more than 90 degrees relative to the ring body. 1. A multi vessel ring comprising:a ring body;a plurality of elongate tubes, each tube having a proximal open end and a distal closed end, each elongate tube being integrally formed with the ring body, and being pivotally connected to the ring body between an initial position in which a longitudinal axis of each tube is generally parallel with an axis of rotation of the ring body, and a final position in which the longitudinal axis ...

Lab-on-disc device

The present invention refers to an analytical device for the analysis of chemical or biological samples comprising a device body, the device body comprising at least one liquid processing unit, the liquid processing unit comprising at least one mixing chamber for mixing at least one sample with at least one reagent, at least one sample dosing chamber for delivering a defined volume of sample to the mixing chamber, at least one reagent channel for delivering at least one reagent to be mixed with the sample, wherein the mixing chamber also serves as detection chamber.

COUNTING, VIABILITY ASSESSMENT, ANALYSIS AND MANIPULATION CHAMBER

DEVICE, METHOD AND APPARATUS FOR IMPLEMENTING THE METHOD, FOR DOSING AT LEAST A PARTICULAR CONSTITUENT IN A PRODUCT SAMPLE

The invention concerns a device for dosing at least a particular constituent in a product sample, comprising a receptacle (110) and a cover (120) assembled to form a closed container. The invention is characterised in that said container has a vertical axis (101), the receptacle and the cover bear coaxial cylindrical walls (111, 112, 113) defining concentric annular chambers (102, 103, 104) inside the container, the walls separating chambers each comprising an opening (11a, 112a), the cover and the container can rotate relatively to each other about the vertical axis and said openings are placed in predetermined manner so that by relative displacement of the walls, the openings are positioned in a straight line or offset to communicate or isolate said successive chambers. The invention also concerns a method for using such a device and an apparatus for implementing said method.

PCR DEVICE

A detection apparatus configured for PCR testing includes a carrier structure (110) and a temperature detection module. The carrier structure is configured to carry a test tube (52), and the test tube (52) is configured for containing a reagent (50, 54) and a specimen. The temperature detection module is disposed on the carrier structure (110) and includes a heat transfer medium (74, 64) and a temperature sensor (144A, 144B). The heat capacity of the heat transfer medium (74, 64) is approximately equal to a preset value, so as to match heat capacity of the reagent (54). The temperature sensor (144A, 144B) is configured to sense a temperature of the heat transfer medium (64). In addition, a method for detecting a temperature of the detection apparatus is also provided.

ИМИТАТОР ТЕПЛОВОГО РАЗДЕЛЕНИЯ ФАЗ

Изобретение относится к имитационному моделированию сепараторов отделения воды от нефти, более конкретно к способу испытания термической добычи. Раскрыт имитатор теплового разделения фаз и способ испытания химических веществ. Имитатор содержит поворотный магазин с кольцевым нагревательным блоком (10), установленным с возможностью поворота на основании (20). Поворотный магазин содержит кольцевой ряд испытательных ячеек (12) для приема множества испытательных флаконов (60), множество нагревательных элементов (14) и термопары (15), расположенные между указанными ячейками. Каждая ячейка имеет канал (40) освещения и вертикальную щель (24), расположенные снаружи для обеспечения возможности визуального наблюдения или отображения вертикального образца флакона. Источник (41) освещения совмещен с каналом освещения каждой ячейки в ответ на поворот поворотного магазина. Способ включает добавление текучей среды со смешанной фазой во множество флаконов, добавление химического вещества в каждый флакон ...

УСТРОЙСТВО, СПОСОБ И ПРИБОР ДЛЯ КОЛИЧЕСТВЕННОГО АНАЛИЗА ПО МЕНЬШЕЙ МЕРЕ ОДНОГО КОНКРЕТНОГО КОМПОНЕНТА В ПРОБЕ ПРОДУКТА

Устройство для анализа по меньшей мере одного компонента в пробе продукта содержит контейнер (110) и крышку (120), которые соединены с образованием закрытого резервуара. Согласно изобретению резервуар имеет вертикальную ось, контейнер и крышка имеют коаксиальные цилиндрические стенки (111, 112, 113), которые разграничивают концентрические кольцеобразные камеры (102, 103, 104) внутри резервуара. Стенки, разделяющие камеры, содержат каждая одно отверстие (111а, 112а). Крышка и контейнер вращаются по отношению друг к другу вокруг вертикальной оси, а отверстия располагаются таким образом, чтобы в результате относительного перемещения стенок они были размещены напротив друг друга или сдвинуты под углом для соединения последовательных камер или их изолирования. Техническим результатом является создание устройства, простого по конструкции и в использовании, которое может работать индивидуально с минимальным числом операций, проводить серийные исследования и в непосредственной близости от места ...

Analysis reagent and analysis device having the analysis reagent carried therein

A reagent including a combination of a polyanionic compound and a bivalent cationic compound contains one substance selected from the group consisting of succinic acid, gluconic acid, alanine, glycine, valine, histidine, maltitol, and mannitol or at least one compound of the substance. A dry state of the reagent and deliquescence can be improved.

Microfluidic device and hemoglobin measurement method using the same

A microfluidic device and a method for measurement of biomaterials using the same. The microfluidic device includes a microfluidic structure including: a sample chamber which receives and accommodates blood; a reagent chamber which contains a luminescent reactant; a first detection chamber which contains a first material that is positively charged; a second detection chamber which is connected to the first detection chamber, and contains a second material having a boronate moiety; and at least one channel which connects the sample chamber, the reagent chamber and the first and second detection chambers.

Devices and methods for interfacing microfluidic devices with macrofluidic devices

The present disclosure is directed generally to devices and methods with the purpose of interfacing microfluidic devices with macrofluidic devices. Specifically, the present disclosure includes the de-

Systems and methods for valving on a sample processing device

A system and method for valving on a sample processing device. The system can include a valve chamber, a process chamber, and a valve septum located between the valve chamber and the process chamber. The system can further include a fluid pathway in fluid communication with an inlet of the valve chamber, wherein the fluid pathway is configured to inhibit a liquid from entering the valve chamber and collecting adjacent the valve septum when the valve septum is in a closed configuration. The method can include rotating the sample processing device to exert a first force on the liquid that is insufficient to move the liquid into the valve chamber; forming an opening in the valve septum; and rotating the sample processing device to exert a second force on the liquid to move the liquid into the valve chamber.

Method and Apparatus for a Microfluidic Device

An microfluidic device and methods for its use, where the microfluidic device comprises: (a) a porous membrane, (b) a gradient layer defining a plurality of gradient micro-channels, where the gradient layer is coupled to a top surface of the membrane, (c) a distributor layer defining a plurality of distributor micro-channels, where the distributor micro-channels are coupled to the plurality of gradient micro-channels, where the distributor layer defines at least one inlet opening and at least one outlet opening, each inlet opening and outlet opening are coupled to the plurality of distributor micro-channels, and (d) self-supporting means coupled to one or more of the porous membrane, the gradient layer and the distributor layer.

Microfluidic apparatus and microfluidic system having the same

A microfluidic system capable of determining at least one of the existence and the amount of a fluid by use of an optical sensor even if the fluid is transparent. The microfluidic system includes a platform including at least one chamber configured to accommodate a fluid, at least one channel connected to the at least one chamber, and at least one protrusion disposed on an inner surface of the at least one chamber and configured to show a difference in transmittance according to the existence of the fluid.

Hydrophilic coating for nonporous surfaces and microfluidic devices including same

A coating formula and method for surface coating non-porous surfaces. Microfluidic devices including said coating achieve desired properties including increased hydrophilicity, improved adhesion, stability and optical clarity.

Centrifugal particle separation and detection device

The present invention provides a centrifugal particle separation and detection device, which can separate and detect particles according to the particle size. The centrifugal particle separate and detection device is practiced with a centrifuge to separate particles, and can be applied to cancer cell detection, blood lymphocyte isolation, tissue engineering, polynucleotide hybridization, microorganism separation and detection and fine chemical purification.

MICROFLUIDIC SYSTEMS FOR EPIDERMAL SAMPLING AND SENSING

A microfluidic system includes a flexible substrate having a skin-facing surface and a back-facing surface; a microfluidic network at least partially embedded in or supported by the flexible substrate; a sensor fluidically connected to the microfluidic network, wherein the microfluidic network is configured to transport a biofluid from a skin surface to the sensor; and a capping layer, having a capping layer skin-facing surface and a back-facing surface, wherein the back-facing surface of the capping layer is attached to the skin-facing surface of the substrate. The flexible substrate is at least partially formed of a thermoplastic elastomer or a polymer configured to provide a high barrier to vapor or liquid water transmission. 1. A microfluidic system , comprising:a flexible substrate having a skin-facing surface and a back-facing surface;a microfluidic network at least partially embedded in or supported by the flexible substrate;a sensor fluidically connected to the microfluidic network, wherein the microfluidic network is configured to transport a biofluid from a skin surface to the sensor; anda capping layer, having a capping layer skin-facing surface and a back-facing surface, wherein the back-facing surface of the capping layer is attached to the skin-facing surface of the substrate;wherein the flexible substrate is at least partially formed of a thermoplastic elastomer or a polymer configured to provide a high barrier to vapor or liquid water transmission.2. The microfluidic system of claim 1 , wherein the flexible substrate and the capping layer have a common additive.3. The microfluidic system of claim 1 , wherein the capping layer is at least partially formed of a thermoplastic elastomer and an additive.4. The microfluidic system of claim 3 , wherein each of the flexible substrate and the capping layer is formed of a common thermoplastic elastomer composition claim 3 , or a different thermoplastic elastomer composition.5. The microfluidic system of claim ...

MICROFLUIDIC VALVE AND METHOD OF MAKING SAME

The present technology provides for a microfluidic substrate configured to carry out PCR on a number of polynucleotide-containing samples in parallel. The substrate can be a single-layer substrate in a microfluidic cartridge. Also provided are a method of making a microfluidic cartridge comprising such a substrate. Still further disclosed are a microfluidic valve suitable for use in isolating a PCR chamber in a microfluidic substrate, and a method of making such a valve. 118.-. (canceled)19. A method of making a microfluidic valve , the method comprising:directing a dispensing head over an inlet hole in a microfluidic substrate;propelling a quantity of thermally responsive substance from the dispensing head into the inlet hole;maintaining a temperature of the microfluidic substrate so that the thermally responsive substance flows by capillary action into a microfluidic channel in communication with the inlet hole; andcovering the inlet hole.20. The method of claim 19 , wherein covering the inlet hole comprises covering two or more inlet holes.21. The method of claim 19 , wherein covering the inlet hole comprises covering two or more inlet holes for valves that are in a sample lane of the microfluidic substrate.22. The method of claim 19 , wherein covering the inlet hole comprises covering two or more inlet holes for valves that are in different sample lanes of the microfluidic substrate.23. The method of claim 19 , wherein covering the inlet hole comprises covering the microfluidic substrate with a layer of plastic.24. The method of claim 19 , wherein covering the inlet hole comprises covering the microfluidic substrate with a layer comprising a pressure sensitive adhesive.25. The method of claim 19 , wherein propelling a quantity of thermally responsive substance comprises propelling a quantity of thermally responsive substance to the bottom of the inlet hole.26. The method of claim 19 , wherein propelling a quantity of thermally responsive substance comprises ...

SAMPLE HOLDING DISC FOR CENTRIFUGATION

A sample holding disc includes a disk-shaped substrate, a plurality of holders for centrifugation including a recess configured to fit a sampling tip in a state where a flow channel of the sampling tip is substantially horizontal and a proximal end side faces the center, and a holder for keeping which includes a groove provided corresponding to each of the holders for centrifugation on an upper surface of the disk-shaped substrate and in which a distal end side of the sampling tip is fitted and held in a state where the proximal end of the sampling tip faces upward. 1. A sample holding disc for centrifugation comprising:a disk-shaped substrate;a plurality of holders for centrifugation comprising a recess provided on an upper surface of the disk-shaped substrate to fit a sampling tip, the sampling tip having an opening, serving as a sample intake port, at its proximal end and including a flow channel holding a sample drawn from the opening, the flow channel having two flow channel portions connected on a distal end side and extending from the distal end side to a proximal end side, one of the flow channel portions leading to the sample intake port, the other flow channel portion terminating at a position not reaching the proximal end, the holder for centrifugation being configured such that the sampling tip is fitted in a state where the flow channel of the sampling tip is substantially horizontal and the proximal end side faces the center; anda holder for keeping which comprises a groove provided corresponding to each of the holders for centrifugation on the upper surface of the disk-shaped substrate and in which the distal end side of the sampling tip is fitted and held in a state where the proximal end of the sampling tip faces upward.2. The sample holding disc for centrifugation according to claim 1 , wherein the holder for keeping is a through groove capable of causing the distal end of the sampling tip to reach a height equal to or lower than a lower surface of ...

Structure for culturing cells

The present invention relates to a structure and a container for culturing cells. In one arrangement, the structure ( 200 ) comprises a partially enclosed cavity ( 204 ) and a plurality of compartments ( 201 ) adapted for fluid communication with the partially enclosed cavity ( 204 ). Each compartment ( 201 ) is configured to hold one or more cell within its compartment ( 201 ) at a location dependent on a dimension of the cell. This arrangement may facilitate visual identification or indication of the size of the cell by observing its location within its compartment ( 201 ). The structure ( 200 ) may be included or formed integrally with a container ( 100 ) such as a Petri dish.

SYSTEM AND METHOD FOR GENERATING OR ANALYZING A BIOLOGICAL SAMPLE

A system including a reaction valve having a mating side and a valve inlet. The reaction valve includes a sample chamber, and the valve inlet is in fluid communication with the sample chamber. The system also has a manifold having an engagement surface. The manifold may include first and second manifold ports at the engagement surface. The engagement surface and the mating side of the reaction valve may be positioned adjacent to each other along an interface. The system may also include a positioning assembly that is operatively coupled to at least one of the reaction valve or the manifold. The positioning assembly is configured to move at least one of the reaction valve or the manifold along the interface to fluidly disconnect the valve inlet from the first manifold port and to fluidly connect the valve inlet to the second manifold port. 133-. (canceled)34. A system comprising:a reaction valve including a sample chamber;a positioning assembly operatively coupled to the reaction valve and configured to rotate the reaction valve about an axis of rotation; andan imaging assembly positioned proximate to the sample chamber of the reaction valve and configured to obtain at least one image of the sample chamber, the positioning assembly configured to rotate the reaction valve to move the sample chamber relative to the imaging assembly, the positioning assembly configured to at least one of (a) rotate the reaction valve after the imaging assembly has imaged the sample chamber or (b) rotate the reaction valve as the imaging assembly images the sample chamber.35. The system of claim 34 , wherein the imaging assembly has an imaging plane claim 34 , the sample chamber moving along the imaging plane as the reaction valve is rotated.36. The system of claim 34 , wherein the at least one image includes first claim 34 , second claim 34 , and third images and the imaging assembly is configured to sequentially obtain the first claim 34 , second claim 34 , and third images claim 34 , ...

MULTI FUNCTION SPINNING PLATFORM

A rotatable platform is provided for use in Lab-on-disc (LoD) applications. A LoD microfluidic device has the rotatable platform and is suitable for analysis of a fluid sample. Real-time monitoring of cells is also provided, using a centrifugal microfluidic platform. A mobile LoD device is provided, which can be used in remote destinations and for point of care applications. A method for monitoring microorganisms under the constant supply of nutrients includes the step of inoculating the cells in a culture chamber in a rotatable microfluidic platform, rotating the platform such that liquid in a reservoir connected to or located on the platform, the liquid comprising nutrients for the cells, is constantly supplied to the cells in the culture chamber by means of shear/centrifugal force resulting from rotating the platform, and continuously monitoring the cells during rotation of the platform by means of imaging, electrochemical and/or electrical measurements. 145-. (canceled)46. A portable centrifugal microfluidic device comprising:a supporting base for supporting the device on a surface;a centrifugal microfluidic disc comprising at least one sample chamber, wherein the microfluidic disc is configured for engaging with a rotatable platform or a motor for spinning the microfluidic disc; an imaging device for imaging a sample in the sample chamber(s); and/or', 'an electrochemical analyser configured for performing electrochemical measurements of a sample in the sample chamber(s); and/or', 'a miniaturized Raman system for in-situ Raman mapping of a sample in the sample chamber(s);, 'a monitoring system comprisingwherein the monitoring system is configured for imaging and/or analysing one or more samples in the sample chamber(s) during rotation of the centrifugal microfluidic disc.47. The portable centrifugal microfluidic device according to claim 46 , wherein the electrochemical analyser is electrically connected to electrodes provided in the sample chamber claim 46 , ...

AUTOMATED ANALYSIS DEVICE

Provided is an automated analysis device with which sufficient reaction process data can be acquired irrespective of the scale of the device, and with which it is possible to ensure freedom of the device configuration. An automated analysis device is provided with: a reaction disk which circumferentially accommodates a plurality of reaction vessels a specimen dispensing mechanism which dispenses a specimen into the reaction vessels a reagent dispensing mechanism which dispenses a reagent into the reaction vessels a measuring unit which measures a reaction process of a mixture of the specimen and the reagent in the reaction vessels and a cleaning mechanism which cleans the reaction vessels after measurement. Further, the automated analysis device includes a controller which controls the drive of the reaction disk such that in one cycle the reaction vessels move by an amount A in the circumferential direction in such a way that N and A are mutually prime, B and C are mutually prime, and the relationship A×B=N×C±1 holds, where N is the total number of reaction vessels accommodated in the reaction disk the reaction disk moves through C (where C>1) rotations+an amount equivalent to one reaction vessel after B (where B>2) cycles, and the number of reaction vessels moved in one cycle is A (where N>A>N/B+1). 1. An automatic analysis device , comprising:a reaction disk which accommodates a plurality of reaction vessels capable of accommodating a dispensed specimen and reagent such that the reaction vessels are spaced apart from each other at predetermined intervals circumferentially;a specimen dispensing mechanism which dispenses a predetermined amount of the specimen into the reaction vessel;a reagent dispensing mechanism which dispenses a predetermined amount of the reagent into the reaction vessel;a measuring unit which measures a reaction liquid during a reaction process and/or after reaction of a mixture of the specimen and the reagent in the reaction vessel;a cleaning ...

SAMPLE PROCESSING AND DETECTING KIT WITH A MATERIAL TRANSFER STRUCTURE

A sample processing and detecting kit with a material transfer structure comprises a kit body defined by an outer shell. A top outer shell of the kit body comprises an opening-and-closing sample port. An inner side of the kit body is disposed with a material circular plate holder and a middle of the material circular plate holder is disposed with a circular hole. A waste liquid storage chamber, a first lock groove, a second lock groove, and a sealing hole are arranged on the material circular plate holder at a periphery of the circular hole. A sample processing plastic tube member is hermitically disposed in the first lock groove. A detection reaction plastic tube member is hermetically disposed in the second lock groove. 1. A sample processing and detecting kit with a material transfer structure , comprising: a top outer shell of the kit body comprises an opening-and-closing sample port,', 'an inner side of the kit body is disposed with a material circular plate holder,', 'a middle of the material circular plate holder is disposed with a circular hole,', 'a waste liquid storage chamber, a first lock groove, a second lock groove, and a sealing hole are arranged on the material circular plate holder at a periphery of the circular hole,', 'a sample processing plastic tube member is hermitically disposed in the first lock groove,', 'a detection reaction plastic tube member is hermetically disposed in the second lock groove,', 'the material circular plate holder is rotatably connected to the material transfer structure through the circular hole,', 'the material transfer structure comprises a hollow rotor disposed with a pipette tip,', 'a bottom end of the hollow rotor is disposed with a hollow pointed structure for piercing a sealing film,', 'a rotatable connection structure surrounds a bottom end of the hollow rotor,', 'a silicone seal disposed below the bottom end of the hollow rotor is disposed in the rotatable connection structure,', 'a spring surrounds an outer ...

MULTI-INDEX DETECTION MICROFLUIDIC CHIP AND METHODS OF USE

A multi-index detection microfluidic chip is provided. A bottom plate () and a cover plate () that matches the bottom plate () and seals it are provided in the microfluidic chip. The center of the microfluidic chip has a through-hole (). The bottom plate () has one or more wave-shaped main channel (s) (), one end of each of the main channel () connected to a sample injection hole () on the bottom plate (), and the other end connected to an exhaust hole () on the bottom plate (). The valley on the main channel () is distal to the through-hole (), and the peak is proximal to the through-hole (). Each valley of the main channel () is connected to a reaction chamber () by a linking channel (). The linking channel () comprises one or more buffering chambers (). The microfluidic chip can be used in detection by fluorescence, turbidity, color, detection equipment, and/or direct observation by the naked eyes. The detection is real-time as the reaction occurs or after the reaction. 1. A microfluidic chip , comprising:(1) a bottom plate comprising:at least one wave-shaped main channel comprising at least one valley and at least one peak;at least one sample inlet port;at least one exhaust port; andat least one reaction chamber,wherein one end of said at least one wave-shaped main channel is connected to said at least one sample inlet port, and the other end is connected to said at least one exhaust port,wherein said at least one valley points away from the center of said bottom plate, and said at least one peak points toward the center of said bottom plate, andwherein said at least one valley of said main channel is connected to said at least one reaction chamber; and(2) a cover plate fittingly engaging said bottom plate.2. The microfluidic chip of claim 1 , wherein said bottom plate further comprises at least one linking channel claim 1 , said linking channel connecting said at least one valley of said main channel to said at least one reaction chamber claim 1 , and wherein ...

Method and Apparatus for Sample Separation and Collection

A centrifuge device and method for use are presented. The centrifuge device includes a housing, a chamber, a channel, and a cover. The housing includes a first port and a vent opening and is designed to rotate about an axis passing through a center of the housing. The chamber is defined within the housing and is coupled to the first port. A first portion of the chamber has a width that tapers between a first width at a first position and a second width at a second position within the chamber, the first width being greater than the second width. The channel is coupled to the second position of the chamber and arranged such that a path exists for gas to travel from the channel to the vent opening. The cover provides a wall that seals the chamber.

Microfluidic Aliquoting For Single-Cell Isolation

According to the invention, generally, a microfluidic aliquoting (MA) chip, adapted to fit in a Petri dish, has a center well (inlet) connected by branched channels to a plurality of side wells (outlets). The chip comes in various types, including a bMA Chip T1, bMA Chip T2, bMA Chip T3, and an rMA Chip. The branched channel improvement provides for a greater distance between neighboring channels and a decreased density near the center well. Design improvements including an injection mold design for an insert and a base and a multiplex hole punch allow for rapid fabrication of the MA chip. 1. A microfluidic aliquot (MA) chip for performing single-cell isolation , comprising:a chip having a radius, a center, a top surface, a bottom surface, an outer edge and a thickness;a single center inlet well disposed substantially at the center of the chip, extending into and accessible from the top surface of the chip;a plurality of side outlet wells disposed in an annular outer portion of the chip, extending into and accessible from the top surface of the chip; anda plurality of channels having multiple segments that extend into the bottom surface of the chip and extending from the center inlet well to the side outlet wells in fluid communication with the center inlet well and the side outlet wells, configured to maintain uniform distribution of a liquid from the center inlet well to the side outlet wells.2. The MA chip of claim 1 , wherein the multiple segments comprise:a plurality of first segments that form an inner layer, a plurality of last segments that form an outer layer, and a plurality of segments between the first segments and the last segments form a middle layer;wherein the side outlet wells and the multiple segments are configured to uniformly distribute liquid and a plurality of cells when the liquid and cells are placed in the MA chip;wherein the first segment is connected to the center inlet well in a radial pattern; andwherein each segment after the first ...

SYSTEMS, DEVICES, AND METHODS FOR ULTRA-SENSITIVE DETECTION OF MOLECULES OR PARTICLES

Described are systems, devices, and methods which related to various aspects of assays for detecting and/or determining a measure of the concentration of analyte molecules or particles in a sample fluid. In some cases, the systems employ an assay consumable comprising a plurality of assay sites. The systems, devices, and/or methods, in some cases, are automated. In some cases, the systems, devices, and/or methods relate to inserting a plurality of beads into assay sites, sealing assay sites, imaging assay sites, or the like. 1153.-. (canceled)154. An apparatus for performing an assay , comprising:{'b': 1', '1, 'a disc handler operatively coupled to an assay consumable disc having a surface comprising wells formed in the surface of the assay consumable disc that have a volume between about femtoliter and about picoliter;'}a first liquid injector positioned to apply a sealing liquid to the surface of the assay consumable disc;a second liquid injector positioned to load an assay sample into the wells of the assay consumable disc;a fluid pump positioned to create a flow of a liquid, which contains a suspension of beads, over the surface of the assay consumable disc to insert beads into the wells;a camera configured to acquire an image of the wells of the assay consumable containing assay sample;a computer implemented controller configured and programmed to operate the first liquid injector, the second liquid injector, and the fluid pump, and to receive the image acquired from the camera; anda motor operated by the computer implemented controller and configured to rotate the assay consumable disc about a central axis of the assay consumable disc.155. The apparatus of claim 154 , wherein each well formed in the surface of the assay consumable disc has a volume between about 1 femtoliter and about 1 picoliter.156. The apparatus of claim 154 , wherein well formed in the surface of the assay consumable disc has a volume between about 30 femtoliters and about 60 femtoliters. ...

ANALYSIS DEVICE, CARTRIDGE AND METHOD FOR TESTING A SAMPLE

An analysis device, a cartridge and a method for analysing a sample are provided, including a rotatable pump head having contact elements that are resiliently biased in the axial direction acting on an elastically deformable pump chamber in order to pump or convey a fluid, such as the sample, a reagent or a gas, when the pump head rotates, in particular in a defined and/or efficient manner. 120-. (canceled)21. An analysis device for testing a sample , comprising:a receptacle for a cartridge containing the sample; anda pump drive for conveying the sample within the cartridge,wherein the pump drive comprises a motor and a pump head configured to be driven by the motor,wherein the pump head comprises a plurality of contact elements for contacting a pump apparatus of the cartridge,wherein the pump head is formed in one piece, and the pump head comprises a base element, wherein the contact elements are each configured to be moved relative to the base element, and', 'the contact elements are configured so that the contact elements rest on or can be brought into contact with the pump apparatus to convey the sample., 'wherein the pump head includes at least one of the following features22. The analysis device according to claim 21 , wherein the contact elements project from the base element claim 21 , and where in the pump head is configured to be rotated by the motor.23. The analysis device according to claim 21 , wherein the contact elements each are resiliently connected to the base element and are configured to be brought into contact with the pump apparatus in a linear manner.24. The analysis device according to claim 21 , wherein the base element is disc-shaped.25. The analysis device according to claim 21 , wherein the contact elements are scoop-like or spoon-like.26. The analysis device according to claim 21 , wherein the contact elements are at least substantially V-shaped or U-shaped in cross section.27. The analysis device according to claim 21 , wherein the ...

REAGENT CARTRIDGE

In one embodiment, a cartridge includes at least one compartment and a reagent in the at least one compartment. The reagent is a chemical composition for testing at least one of soil and vegetation for a chemical contained in the soil or vegetation. The reagent can be used in a soil and/or vegetation analysis test. The cartridge can contain an authentication chip to ensure that the reagent is the correct reagent for the analysis test. 1. A cartridge comprising:at least one compartment;a reagent in the at least one compartment, wherein the reagent is a chemical composition for testing at least one of soil and vegetation for a chemical contained in the soil or vegetation; andwherein the cartridge is adapted to cooperate with a soil and/or vegetation analysis system to supply the reagent to the soil and/or vegetation analysis system.2. The cartridge of claim 1 , further comprising an authentication device.3. The cartridge of claim 2 , wherein the authentication device comprises a chip adapted to be connected to a network claim 2 , wherein when connected to the network claim 2 , the authentication chip is accessed by the network to confirm that the cartridge is an authorized cartridge containing the reagent that is specific for a soil and/or vegetation test.4. The cartridge of further comprising a meter to measure an amount of reagent dispensed from the cartridge claim 1 , wherein the meter communicates the amount of reagent dispensed from the cartridge to a network.5. (canceled)6. The cartridge of further comprising a counter to count a number of times reagent is dispensed from the cartridge to determine consumed volume of reagent claim 1 , wherein the counter communicates with a network to communicate the number of times.7. The cartridge of further comprising a time counter to count time for determining an amount of reagent dispensed from the cartridge claim 1 , wherein the time counter communicates with a network to communicate the amount of time.8. The cartridge of ...

SPECIMEN ANALYSIS SUBSTRATE, SPECIMEN ANALYSIS DEVICE, SPECIMEN ANALYSIS SYSTEM, AND PROGRAM FOR SPECIMEN ANALYSIS SYSTEM

A sample analysis substrate includes a substrate; a first holding chamber; a reaction chamber; a first flow path having a first opening and a second opening respectively connected with the first holding chamber and reaction chamber; a main chamber; a second flow path having a third opening and a fourth opening respectively connected with the reaction chamber and the main chamber; and a magnet accommodation chamber capable of accommodating a magnet. The first opening is located closer to a rotation shaft than the second opening. The second opening is located closer to the rotation shaft than the third opening. The magnet accommodation chamber is located at a position at which, in the case where the magnet is accommodated in the magnet accommodation chamber, the magnet captures magnetic particles in the main chamber. The sample analysis substrate is rotatable to transfer a liquid. 1. A sample analysis substrate rotatable to transfer a liquid , the sample analysis substrate comprising:a substrate including a rotation shaft;a first holding chamber located in the substrate, the first holding chamber having a first space usable to hold a first liquid;a reaction chamber located in the substrate, the reaction chamber having a second space usable to hold a specimen-containing liquid sample;a first flow path located in the substrate, the first flow path having a first opening and a second opening respectively connected with the first holding chamber and reaction chamber;a main chamber located in the substrate, the main chamber having a space usable to hold the specimen-containing liquid sample and magnetic particles having a ligand immobilized to a surface thereof;a second flow path located in the substrate, the second flow path having a third opening and a fourth opening respectively connected with the reaction chamber and the main chamber; anda magnet accommodation chamber located in the substrate, the magnet accommodation chamber being capable of accommodating a magnet; ...

BLOOD CELL COUNTING DEVICE AND METHOD

A device for use in imaging a liquid sample comprises an inlet for accepting the sample, a connection conduit and a detection chamber for detection of the sample, preferably optical detection of the sample. The connection conduit connects the inlet to the detection chamber and contains one or more dry reagents for reaction with the sample as the sample passes through the connection conduit. Specific embodiments include devices arranged for treating a blood sample, in particular lysing and staining the sample. The liquid flow may be driven by capillary effect. The device may further include liquid handling structures arranged for centrifugally driven liquid flow, for example to meter a volume of sample and separate the sample into phases by centrifugation. 1. A device for use in imaging a liquid sample , the device comprising:an inlet for accepting a sample into the device;a connection conduit;a detection chamber for optical detection of the sample,wherein the connection conduit connects the inlet to the detection chamber, andwherein the connection conduit contains one or more dry reagents for reaction with the sample as the sample passes through the connection conduit.2. The device of claim 1 , wherein the sample is a blood sample claim 1 , the one or more dry reagents include a haemolysing agent for selective lysis of erythrocytes in the blood sample and a staining agent for selectively staining leukocytes in the blood sample.3. The device of or claim 1 , wherein the connection conduit comprises a main conduit portion and one or more protrusions extending from the main conduit portion wherein the dry reagents are stored in the one or more protrusions and respective junction regions between the one or more protrusions and the main conduit portion provide a reaction region in which gradual resuspension of the dry reagents can occur.4. The device of claim 3 , wherein the protrusions comprise a main portion and a neck portion in the region of the junction claim 3 , the ...

Paperfuge: An integrated paper-based centrifugation and microfluidics platform for low-cost diagnostics

A microfluidic centrifuge is provided that includes a microfluidic disc having a pair of through holes that include a first through hole and a second through hole, where the pair of through holes are disposed symmetric and proximal to a central axis of the microfluidics disc, where the microfluidics disc includes a sealable input port and a sealable output port, where the sealable input port and the sealable output port are connected by a microfluidics channel, and a pair of tethers disposed through the pair of through holes that are disposed to twist about each other for unwinding where the tether unwinding is disposed to interact with the through holes, where the microfluidics disc rotates about the central axis. 1) A microfluidic centrifuge , comprising a microfluidic disc having a pair of through holes comprising a first through hole and a second through hole , wherein said pair of through holes are disposed symmetric and proximal to a central axis of said microfluidics disc , wherein said microfluidics disc comprises a sealable input port and a sealable output port , wherein said sealable input port and said sealable output port are connected by a microfluidics channel.2) The microfluidics centrifuge of claim 1 , wherein said microfluidics disc comprises a housing material selected from the group consisting of paper claim 1 , polymethyl methacrylate claim 1 , polydimethylsiloxane claim 1 , and plastic.3) The microfluidics centrifuge of further comprises a first tether and a second tether forming a pair of tethers claim 1 , wherein said pair of tethers are disposed through said pair of through holes claim 1 , wherein said pair of tethers are disposed to twist about each said tether for unwinding claim 1 , wherein said tether unwinding is disposed to interact with said through holes claim 1 , wherein said microfluidics disc rotates about said central axis.4) The microfluidics centrifuge of claim 1 , wherein said sealable input port is disposed proximal to said ...

Microfluidic platform and method for controlling the same

A microfluidic platform including a microfluidic layer and a contact layer. The microfluidic layer is embedded with a microfluidic structure including a micro-channel and a fluidic sample contained in the micro-channel. The contact layer is able to be attached to the microfluidic layer, and includes a first heater for heating a first area of the microfluidic structure to a first temperature and a second heater for heating a second area of the microfluidic structure to a second temperature. The microfluidic layer and the contact layer rotate together during operation. A method for controlling a sample in the micro-channel of the microfluidic structure.

Microfluidic device

There is provided a microfluidic device comprising: a plurality of wells, each well comprising one opening to function as an inlet and an outlet for the well, wherein each opening is in fluid communication with a common fluidic channel, and wherein each opening is connected to the common fluidic channel via an isolation channel, and wherein the plurality of wells is arranged on the device in a radially symmetrical pattern. There is also provided a system and method comprising the device.

Testing module and method for testing test sample

A testing module is provided. The testing module includes a carrier, a block member, and a sampling assembly. A flow path connects a storage chamber to a mixing chamber to guide the flow of a fluid. The block member is formed in the flow path to block the fluid from flowing from the storage chamber to the mixing chamber before the connection of the sampling assembly. When the sampling assembly which contains a test sample is connected to the carrier, the fluid mixes with the test sample and flows to the mixing chamber.

MICROFLUIDIC PLATFORM AND METHODS FOR USING THE SAME

The present invention provides a microfluidic device which includes at least 3 chambers, a chamber inlet, at least 2 dichotomously branching generations of channels, a channel inlet, and a channel outlet, wherein the channels and the chambers are separated by deformable walls, wherein each wall is lined with at least one cavity, and wherein the cavity is fluidly connected to the channel.

AUTOMATIC ANALYSER

An automatic analyser which is capable of detecting a dispensing abnormality with a high degree of accuracy without causing the decrease in the determination performance or the increase in the calculation amount caused by the configuration balance of the reference database is implemented. A dispensing nozzle of a sample dispensing mechanism sucks the dispensing target, and internal pressure of the dispensing nozzle when ejecting the dispensing target to a reaction container is detected through a pressure sensor. A plurality of feature quantities are extracted from a waveform of the detected pressure, and a determination result is output through a linear combination formula using an optimal coefficient for a determination function that receives a plurality of feature quantities and outputs one value. The determination result indicates whether or not dispensing of the sample dispensing mechanism is performed normally in accordance with the magnitude of the output result of the determination function. 1. An automatic analyser , comprising:a reaction disk on which a plurality of reaction containers are arranged;a sample dispensing mechanism including a sample dispensing nozzle that sucks a sample contained in a sample container and ejects the sample to a reaction container arranged on the reaction disk;a reagent disk on which a plurality of reagent containers are arranged;a reagent dispensing mechanism including a reagent dispensing nozzle that sucks a reagent contained in the reagent container and ejects the reagent to the reaction container arranged on the reaction disk;a spectroscopic detector that detects light transmitting a solution contained in the reaction container;a pressure sensor that detects internal pressure of the sample dispensing nozzle; anda control unit that controls operations of the reaction disk, the sample dispensing mechanism, the reagent disk, the reagent dispensing mechanism, and the spectroscopic detector,wherein the control unit calculates a ...

MICROFLUIDIC DEVICE AND METHOD OF PRODUCING THE SAME

A microfluidic device and a method of producing the microfluidic device are provided. The microfluidic device includes an upper substrate and a lower substrate fixed to each other to form a microfluidic structure, and a hydrophobic porous layer disposed between the upper substrate and the lower substrate, and configured to fix the upper and lower substrates and absorb air within the microfluidic structure.

Microfluidic device

Provided is a microfluidic device having a structure in which scattering of a sample can be prevented. The microfluidic device includes: a platform including: a sample injection hole, at least one chamber configured to accommodate a sample introduced through the sample injection hole, at least one channel connected to the at least one chamber; and a cover disposed over the sample injection hole, wherein a gap is disposed between the sample injection hole and the cover.

Device and Method for Detecting Analyte by Movement of Particles

The present relates to an analyte detection device comprising: a simple chamber for storing a mixture solution of a sample comprising an analyte and a reactant comprising particles; a detection chamber for storing a detection solution; and a channel placed between the sample chamber and the detection chamber to prevent the mixture solution and the detection solution from being mixed with each other, the analyte detection device characterized by detecting the analyte by moving the particles from the sample chamber to the detection chamber using moving means. 159-. (canceled)60. A method for detecting an analyte using an analyte detection device , the analyte detection device comprising: a sample chamber configured to contain a mixed solution of a sample containing the analyte and a reactant comprising capture particles and labeling particles; a detection chamber configured to contain a detection solution; and a channel located between the sample chamber and the detection chamber so as to prevent the mixed solution and the detection solution from mixing with each other; the method comprising the steps of:mixing the reactant with the sample in the sample chamber to form a capture particle-analyte-labeling particle complex;moving the capture particle-analyte-labeling particle complex to the detection chamber by moving means; anddetecting the analyte in the detection chamber,wherein the capture particles are magnetic particles having immobilized thereon a primary receptor specific for the analyte, and the labeling particles are nonmagnetic particles which have immobilized thereon the primary receptor specific for the analyte and comprise a label.61. The method of claim 60 , wherein the capture particle-analyte-labeling particle complex is moved by applying a magnetic force from the detection chamber to the sample chamber using a magnetic force application unit disposed on one side of the detection chamber.62. The method of claim 60 , wherein the capture particle-analyte- ...

SYSTEM FOR CAPTURING CELLS

The present disclosure relates to a cell extraction device comprising: a plurality of cell extraction modules arranged in a single layer array, the single layer array having a cell-receiving side wherein an opening of at least one of the plurality of cell extraction modules on the cell-receiving surface is configured to receive and retain a single target cell from a fluid sample, and a fluid-evacuating side wherein an opening of the or each of the plurality of cell extraction modules on the fluid-evacuating side is configured to allow fluid from the fluid sample to be evacuated from the cell extraction device, wherein at least one of the plurality of cell extraction modules comprises a micropore capillary configured at a first end that is open on the cell-receiving side to receive a target cell, and configured at a second end that is open on the fluid-evacuating side to allow fluid to pass through. 1. A cell extraction device comprising:a plurality of cell extraction modules arranged in a single layer array, the single layer array having a cell-receiving side wherein an opening of at least one of the plurality of cell extraction modules on the cell-receiving surface is configured to receive and retain a single target cell from a fluid sample, and a fluid-evacuating side wherein an opening of the or each of the plurality of cell extraction modules on the fluid-evacuating side is configured to allow fluid from the fluid sample to be evacuated from the cell extraction device,wherein at least one of the plurality of cell extraction modules comprises a micropore capillary configured at a first end that is open on the cell-receiving side to receive a target cell, and configured at a second end that is open on the fluid-evacuating side to allow fluid to pass through.2. The cell extraction device of claim 1 , wherein the single layer array is arranged over the surface of a planar disc claim 1 , and the surface is provided with a hydrophilic surface configuration.3. The cell ...

HIGH-THROUGHPUT PARTICLE CAPTURE AND ANALYSIS

Microfluidic systems and methods are described for capturing magnetic target entities bound to one or more magnetic beads. The systems include a well array device that includes a substrate with a surface that has a plurality of wells arranged in one or more arrays on the surface. A first array of wells is arranged adjacent to a first location on the surface. A second and subsequent arrays, if present, are arranged sequentially on the surface at second and subsequent locations. When a liquid sample is added onto the substrate and caused to flow, the liquid sample will flow across the first array first and then flow across the second and subsequent arrays in sequential order. The wells in the first array each have a size that permits entry of only one target entity into the well and each well in the first array has approximately the same size. 140-. (canceled)41. A system for capturing target entities that are , or are made to be , magnetic , the system comprising:a body comprising a chamber having an inlet and an outlet; and a substrate;', 'a first array of micro-wells arranged on the substrate at or near the inlet, wherein micro-wells in the first array have a size that permits entry of only one target entity per micro-well and wherein each micro-well in the first array has approximately the same size; and', 'a second array of micro-wells arranged on the substrate at or near the outlet, such that when a liquid is flowed across the substrate from the inlet to the outlet the liquid flows first across the first array and then across the second array, wherein micro-wells in the second array each have a size that is at least ten percent larger than the size of the micro-wells in the first array, and wherein each micro-well in the second array has approximately the same size., 'a micro-well array device arranged within the chamber between the inlet and the outlet, wherein the micro-well array device comprises42. The system of claim 41 , wherein the micro-wells in both the ...

ROTATABLE CARTRIDGE FOR PROCESSING AND ANALYZING A BIOLOGICAL SAMPLE

An automatic analyzer cartridge, spinnable about a rotational axis, has fluid and aliquoting chambers, a metering chamber connected to a vent that is nearer to the rotational axis than the metering chamber, first and second ducts connecting the fluid and aliquoting chambers, and the metering and aliquoting chambers, respectively. Metering chamber side walls taper away from a central region, wherein capillary action next to the walls is greater than in the central region. Fluid flows to the metering chamber using capillary action via the second duct that has an entrance and exit in the aliquoting and metering chambers, respectively; the exit being closer to the rotational axis than the entrance. A downstream fluidic element connects to the metering chamber via a valve. A fluidic structure receives and processes a biological sample into the processed biological sample and has a measurement structure that enables measurement of the processed biological sample. 1. A method of repeatedly dispensing measured amounts of a fluid using a cartridge , wherein the cartridge is operable for being spun around a rotational axis , wherein the cartridge comprises:a metering chamber, wherein the metering chamber is operable for causing fluid to fill the metering chamber using capillary action, wherein the metering chamber has side wall regions and a central region, wherein the side wall regions are narrower than the central region in a cross sectional view of the metering chamber, wherein capillary action next to the side wall regions of the metering chamber is greater than in the central region of the metering chamber; anda duct connected to the metering chamber, wherein the duct comprises a duct entrance and a duct exit in the metering chamber, the duct exit being closer to the rotational axis than the duct entrance, wherein the duct is operable for causing fluid to flow to the metering chamber using capillary action, wherein the method comprises:decreasing a rotational rate of the ...

CHROMATOGRAPHY MEMBRANES, DEVICES CONTAINING THEM, AND METHODS OF USE THEREOF

Described herein are fluid treatment devices for use in tangential flow filtration, comprising a housing unit and a composite material, wherein the composite material comprises: a support member comprising a plurality of pores extending through the support member; and a non-self-supporting macroporous cross-linked gel comprising macropores having an average size of 10 nm to 3000 nm, said macroporous gel being located in the pores of the support member. The invention also relates to a method of separating a substance from a fluid, comprising the step of placing the fluid in contact with an inventive device, thereby adsorbing or absorbing the substance to the composite material contained therein. 2. The fluid treatment device of claim 1 , wherein the composite material is arranged in a substantially coplanar stack of substantially coextensive sheets claim 1 , a substantially tubular configuration claim 1 , or a substantially spiral wound configuration.344-. (canceled)45. The fluid treatment device of claim 1 , wherein the composite material has 2 to 10 separate support members.46. The fluid treatment device of claim 1 , wherein the composite material is a pleated membrane.47. The fluid treatment device of claim 1 , wherein the housing unit is substantially cylindrical.48. The fluid treatment device of claim 1 , wherein the housing unit is disposable or reusable.49. The fluid treatment device of claim 1 , wherein the macroporous cross-linked gel is a neutral or charged hydrogel claim 1 , a polyelectrolyte gel claim 1 , a hydrophobic gel claim 1 , a neutral gel claim 1 , or a gel comprising functional groups.50. The fluid treatment device of claim 49 , wherein the macroporous cross-linked gel is a neutral or charged hydrogel; and the neutral or charged hydrogel is selected from the group consisting of cross-linked poly(vinyl alcohol) claim 49 , poly(acrylamide) claim 49 , poly(isopropylacrylamide) claim 49 , poly(vinylpyrrolidone) claim 49 , poly(hydroxymethyl acrylate) ...

Biological Fluid Collection Device and Biological Fluid Separation and Testing System

A biological fluid collection device that is adapted to receive a blood sample having a cellular portion and a plasma portion is disclosed. After collecting the blood sample, the biological fluid collection device is able to transfer the blood sample to a point-of-care testing device or a biological fluid separation and testing device. After transferring the blood sample, the biological fluid separation and testing device is able to separate the plasma portion from the cellular portion and analyze the blood sample and obtain test results. 1. A biological fluid separation device , comprising:a rotatable body having a center of rotation, an outer periphery, and a body inlet, the body inlet adapted to receive a multi-component blood sample;a separation chamber defined within the rotatable body and in fluid communication with the body inlet and having a chamber outlet spaced apart from the body inlet, the separation chamber adapted to receive the multi-component blood sample; anda blood component chamber defined within the rotatable body and in fluid communication with the chamber outlet,wherein when the separation chamber contains the multi-component blood sample and a rotational force is applied to the rotatable body, a first blood component of the multi-component blood sample passes from the separation chamber into the blood component chamber and a second blood component of the multi-component blood sample is retained within the separation chamber,wherein the blood component chamber is disposed adjacent the center of rotation and the separation chamber is disposed adjacent the outer periphery of the rotatable body, andwherein the body inlet includes an engagement portion configured to receive a transfer device for closed transfer of a multi-component blood sample to the separation device.2. The biological fluid separation device of claim 1 , wherein the first blood component is a plasma component of the multi-component blood sample and the second blood component is a ...

NUCLEIC ACID ANALYZER AND NUCLEIC ACID ANALYZING METHOD

A nucleic acid analyzer includes a first container setting part that sets a first container including reagent storages which store reagents for nucleic acid extraction such that the reagent storages are provided along an X-axis extending in a longitudinal direction of the first container; a dispensing unit that transfers, along the-X axis from the first container, extraction liquid containing nucleic acids extracted in the first container using the reagents for nucleic acid extraction; a second container setting part that is provided along the X-axis and sets a second container, the second container including an injection port through which the extraction liquid transferred by the dispensing unit is injected, storages that store reagents for amplifying the nucleic acids in the extraction liquid, and a flow path that connects the injection port and the storages; and a detector that detects a nucleic acid amplification reaction, which occurs in the storages. 1. A nucleic acid analyzer comprising:a first container setting part that sets a first container including reagent storages which store reagents for nucleic acid extraction such that the reagent storages of the first container are provided along a first axis extending in a longitudinal direction of the first container;a dispensing unit that transfers, along the first axis from the first container, extraction liquid containing nucleic acids extracted in the first container using the reagents for nucleic acid extraction;a second container setting part that is provided along the first axis and sets a second container, the second container including an injection port through which the extraction liquid transferred by the dispensing unit is injected, storages that store reagents for amplifying the nucleic acids in the extraction liquid, and a flow path that connects the injection port and the storages of the second container; anda detector that detects a nucleic acid amplification reaction which occurs in the storages ...

DESIGN OF PAPER SENSOR

The present disclosure proposes a design of a biomedical paper sensor which can determine the concentration of biological materials in fluids such as blood, urine, and saliva. The sensor contains a plurality of axially radiating test zones, each test zone separated from other test zones by wax ink barriers formed by a process that produces thin walls. Each test zone can contain a unique test reagent, or a unique concentration of a test reagent, and can also be identified by printed text. The region of the device outside of the test zone area is printed with a uniform reference color. Benefits of the invention include increased accuracy in the measurement of the concentration of biological materials due to the larger test zone area. Benefits also include the integration of the reference or calibration color which simplifies the calibration needed for quantification of the concentration. 1. A biomedical paper sensor for determining a concentration of biological materials in fluids comprising:a plurality of axial symmetric test zones;wherein the test zones radiate outward from a central point;each of the axial symmetric test zones divided by wax ink barrier walls;each of the axial symmetric test zones contain a unique test reagent therein;a reference region surrounding the plurality of axial test zones; and,wherein the reference region includes a calibration color area including a predeterminable color for comparing to one or more of the axial test zones.2. The paper sensor of claim 1 , wherein the plurality of test zones is at least two.3. The paper sensor of claim 1 , further comprising:a total device area including the combined areas of a reference region area and the axial test zones area; and,wherein the axial test zones area is at least 37.5% of the total device area.4. The paper sensor of claim 1 , wherein an area of each of the axial symmetric test zones is at least 5 mm.5. The paper sensor of claim 1 , wherein the wax ink barrier walls include a thickness of ...

ANALYTICAL CARTRIDGE FOR SOIL TESTING AND RELATED METHODS

A rotary analysis apparatus and related methods are disclosed. The apparatus generally includes a rotary machine operable to rotate or spin a removable disk-type analytical cartridge. The cartridge includes a plurality of fluidly isolated processing trains for processing multiple samples simultaneously. Each process train includes an extractant mixing chamber, slurry filtration chamber, supernatant collection chamber, and reagent mixing chamber in fluid communication. In one use, soil sample slurry is prepared and added to the extractant mixing chamber. The slurry is mixed with an extractant by rotating the cartridge to separate out an analyte from the mixture. A sediment filter in the filtration chamber deliquifies and traps soil particles to produce clear supernatant. A color changing reagent or fluorescent agent may be mixed with the collected supernatant for subsequent colorimetric, fluorescent, turbidimetric, or other type of analysis. 1. An analytical cartridge for fluid testing , the cartridge comprising:a centerline axis;a main body defining a plurality of sample processing trains arranged around the centerline axis, the main body having a mounting opening configured for mounting to a spindle of a rotary machine;each processing train including an extractant mixing chamber having a slurry fill hole for introducing a slurry and an extractant, and a reagent mixing chamber fluidly coupled to the extractant mixing chamber;each processing train including a sediment filter configured to deliquify the slurry for producing a supernatant collected in the reagent mixing chamber for analysis;an annular filter ring separately attached to a bottom of the main body of the cartridge;wherein the filter ring further comprises a plurality of upwardly elongated raised filter housings, each sediment filter being mounted on a respective one of the filter housings.2. The cartridge according to claim 1 , further comprising a sediment filter fluidly interposed between the extractant ...

MICROFLUIDIC STRUCTURE, MICROFLUIDIC DEVICE HAVING THE SAME AND METHOD OF CONTROLLING THE MICROFLUIDIC DEVICE

A microfluidic structure in which a plurality of chambers arranged at different positions are connected in parallel and into which a fixed amount of fluid may be efficiently distributed without using a separate driving source, and a microfluidic device having the same. The microfluidic device includes a platform having a center of rotation and including at least one microfluidic structure. The microfluidic structure includes a sample supply chamber configured to accommodate a sample, a plurality of first chambers arranged in a circumferential direction of the platform at different distances from the center of rotation of the platform, and a plurality of siphon channels, each of the siphon channels being connected to a corresponding one of the first chambers. 1. (canceled)2. A test device , comprising: an accommodating chamber configured to accommodate a fluid;', 'a metering chamber configured to meter an amount of the fluid;', 'a reaction chamber configured to have a chromatographic reaction occur therein using the fluid metered in the metering chamber and introduced therein; and', 'a channel fluidly connecting the accommodating chamber, the metering chamber and the reaction chamber to each other; and, 'a microfluidic device including a platform havinga rotary drive unit configured to rotate the platform of the microfluidic device;a magnet module movable in a radial direction of the platform; anda controller to control the rotary drive unit and the magnet module,wherein the controller, upon transferring the fluid to the metering chamber, stops the platform such that a first order reaction occurs between the fluid and a marker conjugate accommodated in the metering chamber.3. The test device according to claim 2 , wherein the controller is configured to rotate the platform and transfer the fluid accommodated in the accommodating chamber to the metering chamber claim 2 , and repeat intervals comprising increasing rotational speed of the platform and stopping rotation ...

ROTATABLE CARTRIDGE FOR MEASURING A PROPERTY OF A BIOLOGICAL SAMPLE

A cartridge for an automatic analyzer, formed from a cover and carrier structure, is operable for rotation about an axis and has at least one container with at least one reservoir containing at least one fluid. Each container may rotate about the axis within a cavity and relative to the carrier structure. A piercing structure opens a seal of each reservoir when the container rotates relative to the carrier structure. Each container and each cavity has a frictional element that mate and cause friction. Each container has an engaging surface which mates with an engaging surface of a rotational actuator that applies torque to rotate and open the container via the piercing structure. A fluidic structure of the cartridge processes a biological sample into a processed biological sample and enables via a measurement structure measurement of the processed biological sample. A duct is between the cavity and the fluidic structure. 1. A method of performing a measurement of a processed biological sample using a cartridge that is operable for being spun around a rotational axis , the cartridge comprising a carrier structure , a cover provided to the carrier structure , at least one container with at least one fluid reservoir for containing at least one fluid , and a cavity for each of the at least one cartridge , wherein the cavity is formed from the carrier structure and the cover , the at least one container is configured to rotate about the rotational axis of the cartridge within the cavity and relative to the carrier structure , each of the at least one fluid reservoir comprises a pierceable seal , at least one piercing structure for each of the at least one fluid reservoir provided within the cavity , the at least one piercing structure is configured to open the pierceable seal by piercing the pierceable seal upon rotation of the at least one container relative to the carrier structure , the at least one container comprises a first frictional element , a second frictional ...

MICROFLUIDIC CHIP, SYSTEMS, AND METHODS FOR CAPTURING OF ENVIRONMENTAL DNA

A microfluidic lab-on-a-chip (LOC) device, microfluidic systems, and associated methodology are described that allow for intelligently collecting environmental DNA (eDNA) and their associated metadata. Optical spectroscopy is integrated with filtration membranes on the microfluidic device. The microfluidic LOC device and systems can be used for selectively capturing targeted species based on optical characteristics and for recording relevant metadata on eDNA acquired by the filtration membranes. 1. A microfluidic system , comprising:an environmental sample inlet port;a preservative reagent inlet port;a waste outlet port;one or more discrete filter membranes for particle concentration;at least one fluid access port that permits control of the sample fluid stream through a selected filter membrane;at least one optical flow cell that enables at least one of light microscopy, fluorescence spectroscopy, light attenuation measurements, and scattered light intensity measurements, situated before the one or more filter membranes to interrogate the incoming sample fluid stream; andat least one bypass channel to enable disposal of the incoming fluid sample stream and avoid capturing suspended microbes and/or particles on a filter membrane.2. The microfluidic system of claim 1 , wherein the microfluidic system comprises a microfluidic chip having a plurality of layers claim 1 , comprising:an upper layer defining the environmental sample inlet port, the preservative reagent inlet port, the waste outlet port, and in fluid communication with at least one intermediate layer;an intermediate layer in fluidic communication with the one or more filter membranes; anda lower layer in fluid communication with the intermediate layer, incorporating the at least one fluid access port, and the at least one optical flow cell in optical communication with an instrumentation housing.3. The microfluidic system of claim 1 , wherein the at least one optical cell comprises at least one of an ...

LIQUID STORAGE AND RELEASE ASSEMBLY AND LIQUID STORAGE AND RELEASE CHIP

A liquid storage and release chip provided according to the present application includes a structural layer and an elastic cover sheet. An end surface of one side of the structural layer is provided with a liquid storage cell and a liquid quantitation cell, the liquid storage cell and the liquid quantitation cell are separated by a common partition wall, and the partition wall is provided with a valve opening passing through end surfaces of two sides of the structural layer. The elastic cover sheet is attached to the structural layer and covers the liquid storage cell, the liquid quantitation cell and the valve opening in a sealed manner, and the valve opening allows a spool to be inserted in the valve opening in a sealed manner to push up a portion, covering the valve opening, of the elastic cover sheet. 1. A liquid storage and release chip , comprising:a structural layer, wherein an end surface of one side of the structural layer is provided with a liquid storage cell and a liquid quantitation cell, the liquid storage cell and the liquid quantitation cell are separated by a common partition wall, and the partition wall is provided with a valve opening passing through end surfaces of two sides of the structural layer; andan elastic cover sheet attached to the structural layer and covering the liquid storage cell, the liquid quantitation cell and the valve opening in a sealed manner, wherein the valve opening is configured to allow a spool to be inserted in the valve opening in a sealed manner to push up a portion, covering the valve opening, of the elastic cover sheet.2. The liquid storage and release chip according to claim 1 , wherein the structural layer is further provided with a centrifugal positioning hole claim 1 , and the liquid storage cell is located between the liquid quantitation cell and the centrifugal positioning hole.3. The liquid storage and release chip according to claim 1 , wherein a surface claim 1 , attached to the elastic cover sheet claim 1 ...

DEVICES, SYSTEMS AND METHODS FOR SAMPLE DETECTION

This disclosure provides for apparatuses, systems and methods for in vitro sample detection. For example in one embodiment, this disclosure provides an automated Pe-toner microfluidic device (and related method) on a centrifugal platform for DNA sample lysis and DNA extraction. A second embodiment provides a system and method for qualitative detection, quantification, and real-time monitoring of nucleic acid amplification products using magnetic bead aggregation inhibition. A third embodiment provides a platform for simultaneous detection of mRNA markers from blood, cell-free semen, sperm, saliva, and vaginal fluid. The third embodiment comprises a system and method that provide for simple, rapid, and fluorescence-free detection of body fluids using mRNA marker amplification and optical detection for mRNA marker analysis with a smart phone with image analysis. 1. A method to detect a target sequence in a sample , comprising:providing one or more aliquots of an amplification reaction specific for amplifying the target sequence in a sample;contacting the aliquot, magnetic beads and an amount of isolated nucleic acid of greater than about 5 kb in length, optionally under chaotropic conditions, thereby providing a mixture;subjecting the mixture to conditions that allow for aggregation of the beads, wherein the absence of aggregation under the conditions is indicative of the presence of amplified target sequence; anddetecting the presence or amount of aggregation.2. The method of wherein the target sequence is from a pathogen.3. The method of wherein the pathogen is a bacterium claim 2 , virus or parasite.4. The method of wherein the isolated nucleic acid is greater than about 10 kb in length.5. The method of wherein the conditions to allow aggregation are a rotating magnetic field claim 1 , acoustic energy or vibration.6. The method of wherein the aliquot is contacted with the beads before contact with the isolated nucleic acid.7. The method of wherein the aggregation ...

CARTRIDGE FOR SAMPLE ANALYSIS, PRODUCTION METHOD THEREOF, AND USE THEREOF

Disclosed is a cartridge for sample analysis, including a cartridge base body having, formed therein, a chamber configured to store a liquid containing a test substance, and a storage portion which is connectable with the chamber and is configured to store a liquid reagent to be supplied to the chamber, carrier particles immobilized onto an inner wall of the chamber, a first liquid reagent stored in the storage portion, and a first capture substance that specifically binds to the test substance. The carrier particles are immobilized onto the inner wall by means of a solid mixture containing sugar and protein. 1. A cartridge for sample analysis , comprising:a cartridge base body having, formed therein, a chamber configured to store a liquid containing a test substance, and a storage portion which is connectable with the chamber and is configured to store a liquid reagent to be supplied to the chamber;carrier particles immobilized onto an inner wall of the chamber;a first liquid reagent stored in the storage portion; anda first capture substance that specifically binds to the test substance, whereinthe carrier particles are immobilized onto the inner wall by means of a solid mixture containing sugar and protein.2. The cartridge of claim 1 , wherein a mass ratio of the sugar to the protein in the solid mixture is not less than 0.1 and not greater than 100.3. The cartridge of claim 2 , wherein the mass ratio of the sugar to the protein in the solid mixture is not less than 0.5 and not greater than 30.4. The cartridge of claim 1 , wherein a mass ratio of the carrier particles to the protein contained in the solid mixture is not less than 0.05 and not greater than 10.5. The cartridge of claim 1 , whereinthe carrier particles are obtained by drying, on the inner wall of the chamber, a suspension of the carrier particles which is obtained by dispersing the carrier particles into the solution containing sugar and protein, anda concentration of the protein in the suspension ...

Devices and methods for detection of biomarkers

Devices for detecting biomarkers in a sample and methods for detecting such biomarkers are discussed. The biomarkers may be associated with a particular disease such as breast cancer or another health condition. The devices may contain a disc including a plurality of microfluidic channels extending in a radial direction of the disc, the microfluidic channels pre-loaded with a plurality of capture molecules specific to at least one biomarker. The methods may include introducing a fluid sample into at least one microfluidic channel of a disc, rotating the disc, such that the fluid sample flows radially outward through the at least one microfluidic channel to combine with capture molecules specific to at least one biomarker, and detecting a signal from the disc indicative of a presence of the at least one biomarker contained in the fluid sample.

MICROFLUIDIC VALVE AND METHOD OF MAKING SAME

The present technology provides for a microfluidic substrate configured to carry out PCR on a number of polynucleotide-containing samples in parallel. The substrate can be a single-layer substrate in a microfluidic cartridge. Also provided are a method of making a microfluidic cartridge comprising such a substrate. Still further disclosed are a microfluidic valve suitable for use in isolating a PCR chamber in a microfluidic substrate, and a method of making such a valve. 118.-. (canceled)19. A method of making a microfluidic valve in a microfluidic device comprising a reactor and a window disposed relative to the reactor to allow detection of fluorescence from the reactor , the method of making comprising:directing a dispensing head over an inlet hole in a microfluidic substrate;propelling a quantity of thermally responsive substance as a succession of dots from the dispensing head into the inlet hole; andmaintaining a temperature of the microfluidic substrate so that the thermally responsive substance flows by capillary action from the inlet hole into a microfluidic loading channel in communication with the inlet, the microfluidic loading channel comprising a first end and a second end, the second end of the microfluidic loading channel intersecting a microfluidic flow channel at a T-junction such that the flow of thermally responsive substance stops when the thermally responsive substance reaches the microfluidic flow channel without entering the microfluidic flow channel.20. The method of claim 19 , wherein the T-junction intersection between the microfluidic loading channel and the microfluidic flow channel comprises a higher cross section than the microfluidic loading channel.21. The method of claim 19 , wherein capillary forces in the T-junction intersection between the microfluidic loading channel and the microfluidic flow channel are lower than in the microfluidic loading channel.22. The method of claim 19 , wherein the quantity of thermally responsive ...

MICROFLUIDIC APPARATUS

A microfluidic apparatus mounted on a rotation driving unit for inducing a fluid to flow due to a centrifugal force includes: a target chamber that houses a first fluid; a first chamber that houses a second fluid and is disposed closer to a rotation center of the microfluidic apparatus in a radius direction than the target chamber, the first chamber being connected to the target chamber by a first channel; a first valve that prevents a flow of the second fluid through the first channel; and a second chamber disposed closer to the rotation center in the radius direction than the target chamber and connected to the target chamber by a second channel, wherein the first fluid is transported to the second chamber by supplying the second fluid to the target chamber by the centrifugal force. 1. A microfluidic apparatus for inducing a fluid to flow due to a centrifugal force , the apparatus comprising:a target chamber that houses a first fluid;a first chamber that houses a second fluid and is disposed closer to a rotation center of the microfluidic apparatus in a radius direction than the target chamber, the first chamber being connected to the target chamber by a first channel;a first valve that controls a flow of the second fluid through the first channel; anda second chamber disposed closer to the rotation center in the radius direction than the target chamber and connected to the target chamber by a second channel,wherein the first fluid is transported from the target chamber to the second chamber by supplying the second fluid to the target chamber from the first chamber by the centrifugal force.2. The microfluidic apparatus of claim 1 , further comprising a second valve that controls the flow of the first fluid through the second channel.3. The microfluidic apparatus of claim 1 , wherein the first fluid and the second fluid do not mix with each other.4. The microfluidic apparatus of claim 1 , wherein the first channel is filled with a gas.5. The microfluidic apparatus ...

MICROFLUIDIC DEVICE AND METHOD FOR RAPID HIGH THROUGHPUT IDENTIFICATION OF MICROORGANISMS

An apparatus and method are disclosed for detecting the presence of a microorganism within sampling device. The sampling device has a plurality of reaction chambers each having a reactive reagent for reacting with the microorganism to indicate the presence of the microorganism within the reaction chamber. A grabber holding the sampling device and a motion stage connected to the grabber moves the sampling device in a plane. A detector detects each of the plurality of reaction chambers for detecting the presence of the microorganism within the reaction chamber. 1. An apparatus for detecting a microorganism within sampling device , the sampling device having a plurality of reaction chambers each having a reactive agent for reacting with the microorganism to indicate the presence of the microorganism in said reaction chamber , comprising:a grabber for holding the sampling device;a motion stage connected to said grabber for moving the sampling device in a plane; anda detector for detecting each of the plurality of reaction chambers for detecting the presence of the microorganism in said reaction chamber.2. The apparatus for detecting the Microorganism within sampling device as set forth in claim 1 , wherein the sampling device is a rotatable disk having a central hole; andsaid grabber engaging with the central hole for holding and rotating said rotary disk.3. The apparatus for detecting the Microorganism within sampling device as set forth in claim 1 , wherein said detector is a spectrometer for detecting the presence of the microorganism in the reaction chamber.4. The apparatus for detecting the Microorganism within sampling device as set forth in claim 1 , wherein said detector includes a light source located on one side of the sampling device and a spectrometer located on another side of the sampling device for detecting the presence of the microorganism in the reaction chamber.5. The apparatus for detecting the Microorganism within sampling device as set forth in ...

MICROFLUIDIC ROTOR DEVICE

Described herein are various embodiments directed to rotor devices, systems, and kits. Embodiments of rotors disclosed herein may be used to characterize one or more analytes of a fluid. An apparatus may include a first layer being substantially transparent. A second layer may be coupled to the first layer to collectively define a set of wells. The second layer may define a channel, and the second layer may be substantially absorbent to infrared radiation. A third layer may be coupled to the second layer. The third layer may define an opening configured to receive a fluid. The third layer may be substantially transparent. The channel may establish a fluid communication path between the opening and the set of wells. 1. An apparatus , comprising:a first layer being substantially transparent; anda second layer coupled to the first layer to collectively define a set of wells, the second layer defining a channel, the second layer being substantially absorbent to infrared radiation; anda third layer coupled to the second layer, the third layer defining an opening configured to receive a fluid, the third layer being substantially transparent, wherein the channel establishes a fluid communication path between the opening and the set of wells.2. The method of claim 1 , wherein the first layer and the third layer are each independently transparent.3. The apparatus of claim 1 , wherein substantially transparent includes light transmission of at least one of ultraviolet light claim 1 , visible light claim 1 , and infrared radiation.4. The apparatus of claim 1 , wherein the second layer is substantially absorbent to at least one of mid-infrared radiation and near-infrared radiation.5. The apparatus of claim 1 , wherein substantially absorbent to infrared radiation includes absorbing infrared radiation in a sufficient amount within a predetermined period of time to transition a portion of the first layer and the third layer from a solid phase to a molten phase.6. The apparatus of ...

Blood Plasma Separation Device

A device for separating blood plasma from whole blood includes a first reservoir and a second reservoir. The first reservoir is configured to receive a sample of whole blood including red blood cells and includes a collection region and a constricted region. The second reservoir is fluidically connected to the constricted region of the first reservoir, such that, responsive to centrifugal force applied to the device, the sample of whole blood disposed within the first reservoir separates into a first fraction and a second fraction. The first fraction is located in the collection region and includes blood plasma from which substantially all red blood cells have been removed. The second fraction is located in the second reservoir and includes blood plasma and red blood cells that have been removed from the first fraction by the centrifugal force. The constricted region inhibits the second fraction from entering the collection region.

Biotip

A biotip according to an embodiment of the invention moves a reaction mixture along a longitudinal direction under the force of gravity. The biotip includes a chamber formed of a transparent material and filled with a liquid having a smaller specific gravity than the reaction mixture and immiscible with the reaction mixture, and a seal that seals the chamber. The liquid has a volume resistivity of greater than 0 Ω·cm and 5×10 13 Ω·cm or less.

Microfluidic Aliquot Chip For Single-Cell Isolation

According to the invention, generally, a microfluidic aliquot (MA) chip, adapted to fit in a Petri dish, has a center well (inlet) connected by branched channels to a plurality of side wells (outlets). The chip comes in various types, including a bMA Chip T1, bMA Chip T2, bMA Chip T3, and an rMA Chip. The branched channel improvement provides for a greater distance between neighboring channels and a decreased density near the center well. An insert and a base are configured to create an MA chip. 1. A microfluidic aliquot (MA) chip for isolating cells , comprising:a chip having a center, a top surface, a bottom surface, an outer edge and a thickness;an inlet well disposed substantially at the center of the chip, extending into and accessible from the top surface of the chip;a plurality of outlet wells disposed in an outer portion of the chip, extending into and accessible from the top surface of the chip;a plurality of multiple segments that extend from the inlet well to the outlet wells;wherein the multiple segments comprise branched channels; andwherein the chip is configured to maintain uniform distribution of a liquid and cells from the inlet well to the outlet wells.2. The MA chip of claim 1 , wherein the chip comprises:a plurality of first segments that form an inner section, a plurality of last segments that form an outer section, and a plurality of segments between the first segments and the last segments that form a middle section;wherein the first segment is connected to the center inlet well in a radial pattern;wherein each segment after the first segment is divided from a prior segment in a radial pattern;wherein a first channel of a last segment is shorter than a second channel of the last segment; andwherein every other outlet well is disposed farther away from the inlet well than an adjacent outlet well.3. The MA chip of claim 2 , wherein a plurality of segments in the middle section form a curved portion generally in the shape of a bend claim 2 , ...

Methods For Making A Microfluidic Aliquot Chip

According to the invention, generally, a method for making a microfluidic aliquoting (MA) chip, adapted to fit in a Petri dish, has a center well (inlet) connected by branched channels to a plurality of side wells (outlets). The chip comes in various types, including a bMA Chip T1, bMA Chip T2, bMA Chip T3, and an rMA Chip. The branched channel improvement provides for a greater distance between neighboring channels and a decreased density near the center well. Design improvements including an injection mold design for an insert and a base and a multiplex hole punch allow for rapid fabrication of the MA chip. 1. A method for making an MA chip , comprising:inserting a first pair of patterned metal injection molds into an injection molding machine;injecting thermoplastic into the first pair of patterned metal injection molds;demolding to produce an insert having a reverse pattern of the first pair of molds;inserting a second pair of patterned metal injection molds into the injection molding machine;inserting thermoplastic into the second pair of patterned metal injection molds;demolding to produce a base having a reverse pattern of the second pair of molds;inserting a plurality of rivets on the base into a plurality of rivet through-holes on the insert; andsealing a center through-hole, branched channels, and aliquoting through-holes disposed on the insert when the rivets are inserted into the rivet through-holes.2. A method for creating holes in an MA chip , comprising:inserting an MA chip between a top and a bottom of an enclosure;aligning the MA chip directly above a reverse mold in the enclosure;inserting a plurality of pins attached to a bottom of a pin head into a plurality of tapered holes on the top of the enclosure;pressing a top of the pin head so that the MA chip is pushed into the reverse mold; andwherein the MA chip and enclosure are aligned when the reverse mold has a pattern that matches a pattern on the MA chip and the reverse mold has a flattened edge ...

SPECIMEN PROCESSING METHOD, SPECIMEN PROCESSING APPARATUS, NON-TRANSITORY COMPUTER-READABLE STORAGE MEDIUM, AND SPECIMEN PROCESSING CARTRIDGE

According to one or more aspects, a specimen processing method may use a cartridge comprising a chamber configured to store a liquid. The method may include: storing a specimen and a dispersion medium in the chamber of the cartridge; and rotating the cartridge about a rotational shaft to agitate the specimen and the dispersion medium in the chamber, to thereby form an emulsion in which a dispersoid containing the specimen is dispersed in the dispersion medium. 1. A specimen processing method that uses a cartridge comprising a chamber configured to store a liquid , the method comprising:storing a specimen and a dispersion medium in the chamber of the cartridge; androtating the cartridge about a rotational shaft to agitate the specimen and the dispersion medium in the chamber, to thereby form an emulsion in which a dispersoid containing the specimen is dispersed in the dispersion medium.2. The specimen processing method according to claim 1 , wherein the cartridge comprises a plurality of chambers arranged in an arc form whose radial distances from the rotational shaft are substantially equal.3. The specimen processing method according to claim 1 , whereinthe rotating the cartridge about the rotational shaft to form the emulsion comprises changing a rotational speed of the cartridge repeatedly.4. The specimen processing method according to claim 3 , wherein reversing a rotational direction of the cartridge; or', 'accelerating and decelerating the rotational speed of the cartridge in one direction., 'the changing the rotational speed of the cartridge comprises5. The specimen processing method according to claim 4 , whereinthe changing the rotational speed of the cartridge comprises reversing the rotational direction of the cartridge in a cycle of 165 milliseconds or more and 330 milliseconds or less.6. The specimen processing method according to claim 3 , whereina ratio of a total volume of the dispersoid and the dispersion medium to the chamber is 30% or more and 70% ...

ANALYSIS CHIP AND SAMPLE ANALYSIS APPARATUS

Provided are an analysis chip and a sample analysis device by which it is possible to perform analysis rapidly and accurately on a plurality of items simultaneously using a small amount of liquid for measurement. An analysis chip () is provided with: a substrate () formed in a substantially disc shape; an insertion hole () formed in the center of the substrate (), a liquid to be measured being inserted into the insertion hole (); and micro-flow paths () into which the liquid to be measured can be guided from the insertion hole () by capillary action, each of the micro-flow paths () having therein a plurality of types of antigens () that are fixed in place with gaps therebetween, the antigens () selectively reacting to components in the liquid to be measured. 1. An analysis chip comprising: a substrate formed into a substantially disc shape;an injection port formed at the center of the substrate and through which target liquid as a measurement target is injected; anda flow path that allows introduction of the target liquid into the flow path through the injection port by means of capillary action, wherein multiple types of reactants capable of selectively reacting with a component in the target liquid are fixed to the flow path.2. The analysis chip according to claim 1 , wherein the flow path of the analysis chip includes a plurality of flow paths arranged in a radial pattern to extend to an outer edge of the substrate claim 1 , and the multiple types of reactants capable of selectively reacting with a component in the target liquid are fixed to each of the flow paths to be arranged at given intervals.3. The analysis chip according to claim 1 , wherein the analysis chip is formed in such a manner that the flow path surrounds the injection port.4. The analysis chip according to claim 1 , wherein the reactants are fixed in the form of spots to the flow path.5. The analysis chip according to claim 1 , wherein the reactants fixed to the flow path each have a shape like a ...

Method for preparing monolithic coated surfaces

A carrier for adsorption a compound, comprising a support; and a shrink-fitted monolithic body attached to and surrounding at least a portion of the support. The monolithic body can be porous and configured to bind compounds in a solution either for the isolation or depletion of the compounds from the solution.

Devices and methods for extraction, separation and thermocycling

A method to extract, amplify and separate nucleic acid in a microfluidic device having a plurality of chambers and channels can include a) introducing cells having nucleic acid to a first chamber of the microfluidic device and subjecting the cells in the first chamber to conditions that lyse the cells. The method can further include b) subjecting the first chamber to centrifugal force, thereby allowing the lysate or a portion thereof having nucleic acid to be distributed to a second chamber through a first channel in the microfluidic device. The method can also include c) combining the lysate or the portion thereof and reagents for amplification of the nucleic acid, thereby providing a second mixture. The method can also include d) subjecting the second chamber to centrifugal force, thereby allowing gas to be expelled from the second mixture.

SELF-DIGITIZATION OF SAMPLE VOLUMES

Devices, systems and apparatuses for the discretization and manipulation of sample volumes are provided. Related methods are also provided. 1. A microfluidic device comprising:a microwell plate comprising a plurality of wells;a fluid inlet port in fluidic communication with the well;a fluid outlet port in fluidic communication with the well;a flow channel having a flow axis, the flow channel in fluidic communication with the fluid inlet port and the fluid outlet port; anda plurality of fluidic harbors in fluidic communication with the flow channel and offset from the flow axis.2. The microfluidic device of claim 1 , wherein at least one well of the plurality of wells comprises a plurality of the flow channels.3. The microfluidic device of claim 1 , wherein at least one well of the plurality of the wells comprises a plurality of fluid inlet ports including the fluid inlet port.4. The microfluidic device of claim 1 , wherein at least one well of the plurality of the wells comprises a plurality of fluid outlet ports including the fluid outlet port.5. The microfluidic device of claim 1 , wherein the plurality of wells is arranged in an array.6. The microfluidic device of claim 1 , wherein the plurality of wells is arranged in a square matrix array.7. The microfluidic device of claim 1 , wherein the plurality of wells comprises 6 claim 1 , 12 claim 1 , 24 claim 1 , 48 claim 1 , 96 claim 1 , 384 claim 1 , or 1536 wells.8. The microfluidic device of claim 1 , wherein at least one fluidic harbor of the plurality of fluidic harbors is at an angle other than orthogonal to the flow axis.9. The microfluidic device of claim 1 , wherein at least one fluidic harbor of the plurality of fluidic harbors is at an angle orthogonal to the flow axis.10. The microfluidic device of claim 1 , wherein each fluidic harbor of the plurality of fluidic harbors is in fluidic communication with the flow channel by an opening conduit.11. The microfluidic device of claim 1 , wherein at least one ...

OPTICAL DENSITY INSTRUMENT AND SYSTEMS AND METHODS USING THE SAME

Instruments, systems, and methods for measuring optical density of microbiological samples are provided. In particular, optical density instruments providing improved safety, efficiency, comfort, and convenience are provided. Such optical density instruments include a handheld portion and a base station. The optical density instruments may be used in systems and methods for measuring optical density of biological samples. 130.-. (canceled)31. An optical density testing apparatus , the apparatus comprising:a housing configured to receive at least a portion of a sample tube;an emitter configured to emit light towards the sample tube;at least one sensor configured to receive the emitted light from the emitter and generate a signal indicative of an intensity of the received light; anda wireless transmitter configured to wirelessly transmit optical density data to a receiving device based upon the signal indicative of the intensity of the received light.32. The optical density testing apparatus according to claim 31 , wherein the wireless transmitter is configured to transmit the optical density data directly to the receiving device.33. The optical density testing apparatus according to claim 31 , wherein the wireless transmitter is configured to transmit the optical density data to the receiving device via a wireless communication network.34. The optical density testing apparatus according to claim 31 , wherein the wireless transmitter is configured to transmit the optical density data to the receiving device in real time.35. The optical density testing apparatus according to claim 31 , wherein the wireless transmitter is configured to wirelessly transmit the optical density data to the receiving device continuously during at least a portion of a testing operation during which the emitted light is received by the at least one sensor.36. The optical density testing apparatus according to claim 35 , wherein the continuous wireless transmission of the optical density data ...

METHOD, APPARATUS, AND COMPUTER PROGRAM PRODUCT FOR CONTROLLING COMPONENTS OF A DETECTION DEVICE

A method, computer program product, and apparatus are provided for controlling components of a detection device. The device may detect turbidity of liquid with sensors such as a density sensor and/or nephelometric sensor. A light modulation pattern may reduce or eliminate interference in sensor readings. Readings may be performed during off cycles of an illumination light to reduce interference but to provide improved visibility of a tube. Dark and light sensor readings may be performed with an emitter respectively off or on to account for ambient light in subsequent readings. Readings from the density sensor and/or nephelometric sensor may be used to calculate McFarland values. The device may be zeroed based on an emitter level that results in a sensor reading satisfying a predetermined criterion. 124-. (canceled)25. An apparatus for reducing light interference during testing contents of a sample tube , the apparatus comprising:a shell defining one or more cavities for receiving at least a portion of a sample tube;an illumination light for illuminating the sample tube with visible light for a plurality of illumination light activation periods;an emitter configured to emit a source light towards the sample tube; andone or more sensors configured to receive at least a portion of the emitted source light during a plurality of sensor reading periods,wherein the plurality of illumination light activation periods and the plurality of sensor reading periods are configured to be sequenced such that, for at least a period of time, the illumination light and the one or more sensors appear to be simultaneously illuminating the sample tube and receiving at least a portion of the emitted source light, respectively, while the plurality of illumination light activation periods and the plurality of sensor reading periods do not overlap.26. The apparatus of claim 25 , wherein a first sensor reading period of the plurality of sensor reading periods is initiated based at least in ...

APPARATUS FOR ANALYTE EXAMINATION

An apparatus comprises a biosensor disk structure including a first substrate with a first inner surface, a second substrate with a second inner surface facing oppositely toward the first inner surface, and fluidic channels reaching between the first and second inner surfaces; wherein the first inner surface has binding sites and non-binding sites adjoining the binding sites, the first substrate is transparent at the non-binding sites, and the non-binding sites have discrete polygonal configurations of equal size and shape; and the second inner surface has non-reflective areas and reflective areas bounded by the non-reflective areas, and the reflective areas have discrete polygonal configurations sized and shaped equally with the non-binding sites such that the reflective areas are located coextensively opposite the non-binding sites. 1. An apparatus comprising: a first substrate with a first inner surface,', 'a second substrate with a second inner surface facing oppositely toward the first inner surface, and', 'fluidic channels reaching between the first and second inner surfaces;, 'a biosensor disk structure including'}wherein the first inner surface has binding sites and non-binding sites adjoining the binding sites, the first substrate is transparent at the non-binding sites, and the non-binding sites have discrete polygonal configurations of equal size and shape; and the second inner surface has non-reflective areas and reflective areas bounded by the non-reflective areas, and the reflective areas have discrete polygonal configurations sized and shaped equally with the non-binding sites such that the reflective areas are located coextensively opposite the non-binding sites.2. The apparatus of claim 1 , wherein the non-reflective areas are contiguous around and between the reflective areas.3. The apparatus of claim 1 , wherein the non-reflective areas are sized and shaped equally with the binding sites and the binding sites and non-reflective areas are located ...

Thermal cycler

A biotip according to an embodiment of the invention moves a reaction mixture along a longitudinal direction under the force of gravity. The biotip includes a chamber formed of a transparent material and filled with a liquid having a smaller specific gravity than the reaction mixture and immiscible with the reaction mixture, and a seal that seals the chamber. The liquid has a volume resistivity of greater than 0 Ω·cm and 5×10 13 ∩·cm or less.

MICROFLUIDIC ANALYTICAL DEVICE FOR ANALYSIS OF CHEMICAL OR BIOLOGICAL SAMPLES, METHOD AND SYSTEM THEREOF

An analytical device for analysis of chemical or biological samples, a method of using such a device, based on rotation of the device, integrated sample dosing and optical detection, and a system comprising such a device are disclosed. The analytical device comprises a device body having a liquid processing unit. The liquid processing unit comprises a mixing chamber for mixing a sample with a reagent, a sample dosing chamber for delivering a defined volume of the sample to the mixing chamber, and a reagent channel for delivering the reagent to be mixed with the sample, wherein the mixing chamber also serves as a detection chamber. 1. A method for analysis of chemical or biological samples comprising:providing an analytical device comprising a device body, the device body comprising at least one liquid processing unit, the liquid processing unit comprising at least one mixing chamber for mixing at least one sample to be analyzed with at least one reagent, the at least one mixing chamber being at least partially transparent, at least one sample dosing chamber in fluid connection with the mixing chamber for delivering a defined volume of the sample to the mixing chamber, at least one reagent channel in fluid communication with the mixing chamber for delivering the at least one reagent to be mixed with the sample, and at least one waste chamber;introducing into said analytical device the at least one sample to be analyzed;rotating via a rotor the analytical device at a rotational speed so that the sample dosing chamber is filled with the volume of the sample to be analyzed while an excess of sample is guided into the waste chamber;increasing the rotational speed to let the sample in the sample dosing chamber pass into the mixing chamber;introducing at least one reagent into said analytical device;rotating via the rotor the analytical device at a rotational speed so that the at least one reagent is guided into the mixing chamber,performing a reciprocating rotary motion ...

Systems and Methods for Multianalyte Detection

Provided herein are systems, devices and methods for performing multianalyte detection in a biological sample, such as a human blood sample. Multiwell plates useful for performing multianalyte detection are also provided. The systems, devices and methods provided herein relate to the field of direct-to-consumer diagnostics (DTC diagnostics) and are useful, e.g., for facilitating consumer access to consumer healthcare and consumer wellness information. Other uses of the systems, devices and methods provided herein relate to the fields of medical research and drug discovery. 1133-. (canceled)134. A system comprising:a light source; a fluorescence detection module;', 'an absorbance reader; and', 'a cell-enumeration module;, 'a detector comprisinga platform configured for movement of a multiwell plate underneath the fluorescence detection module, absorbance reader, and/or the cell-enumeration module, the multiwell plate having a plurality of wells disposed therein that are configured to receive a liquid sample;a liquid handling device to transfer liquids to the wells of the multiwell plate; anda housing encapsulating each of the light source, detector, liquid handling device, and platform.135. The system of claim 134 , wherein the platform is configured to rotate the multiwell plate underneath each of the fluorescence detection module claim 134 , absorbance reader claim 134 , and the cell-enumeration module.136. The system of claim 134 , wherein the liquid handling device is a piezoelectric or acoustic liquid dispenser configured to transfer aqueous liquid from a liquid reservoir within the housing into a well of the multiwell plate.137. The system of further comprising the multiwell plate.138. The system of claim 137 , wherein the plurality of wells are organized in concentric circles around a center of the multiwell plate.139. The system of claim 138 , wherein the plurality of wells is arranged in a spokes-like arrangement.140. The system of claim 139 , wherein the ...

OPTICAL TEST PLATFORM

Provided herein are an optical test platform and corresponding method of manufacturing the same. The test platform may include a shell defining a cavity for receiving a sample tube, a first aperture, and a second aperture. The first aperture and the second aperture of the shell may each be configured to optically couple the cavity with an exterior of the shell. The test platform may further include a first window and a second window embedded in the shell. The first window may seal a first aperture and the second window may seal a second aperture. The first window and second window may each permit the optical coupling of the cavity with the exterior of the shell. The first window and the second window may be optically coupled via the cavity, and the shell may prohibit optical coupling between the first window and the second window through the shell.

CHIP FOR SAMPLE DETECTION AND PACKAGING METHOD THEREOF

A chip for sample detection includes a substrate, an upper covering plate disposed above the substrate and a lower covering plate disposed under the substrate. A gap between an upper end face of the substrate and the upper covering plate is sealed, a gap between a lower end face of the substrate and the lower covering plate is sealed, and the substrate is provided with a via hole penetrating the upper end face and the lower end face. 1. A chip for sample detection , comprising:a substrate;an upper covering plate disposed above the substrate; anda lower covering plate disposed under the substrate,wherein a gap between an upper end face of the substrate and the upper covering plate is sealed, a gap between a lower end face of the substrate and the lower covering plate is sealed, and the substrate is provided with a via hole penetrating the upper end face and the lower end face.2. The chip for sample detection of claim 1 , wherein the chip is a microfluidic chip claim 1 , and the via hole comprises one or more of a micro channel claim 1 , a reaction chamber and a detection chamber.3. The chip for sample detection of claim 1 , wherein the gap between the upper end face of the substrate and the upper covering plate is directly sealed claim 1 , and/or the gap between the lower end face of the substrate and the lower covering plate is directly sealed.4. The chip for sample detection of claim 1 , wherein the gap between the upper end face of the substrate and the upper covering plate is sealed by a medium claim 1 , and/or the gap between the lower end face of the substrate and the lower covering plate is sealed by a medium.5. The chip for sample detection of claim 4 , wherein the medium is composed of an adhesive layer.6. The chip for sample detection of claim 5 , wherein the adhesive layer is made of a pressure-sensitive double-sided adhesive claim 5 , a UV-Curing adhesive or an optical grade double-sided adhesive.7. The chip for sample detection of claim 1 , wherein the ...

DEVICE FOR STORING AND TRANSPORTING TISSUE SPECIMENS

A container comprising: 1. A container comprising:a first panel;a second panel opposing the first panel;a first side;a second side opposing the first side;a third side;a fourth side opposing the third side;wherein each of the sides joins the first panel and the second panel;a chamber defined by the four sides, the first panel, and the second panel, wherein the chamber is configured to receive a biological specimen;a port defined in the first side;a plug inserted into the port;an opening defined in the second side; anda closure element coupled to the opening,wherein the chamber is leak proof.2. The container of claim 1 , wherein at least one of the first panel or the second panel is transparent.3. The container of claim 1 , wherein both the first panel and the second panel are transparent.4. The container of claim 1 , wherein the closure element is perforated with at least one hole that communicates with the opening in the second side.5. The container of claim 1 , wherein the closure element is removably coupled to the opening of the second side.6. The container of claim 1 , wherein the container is in the shape of a rectangular cuboid.7. The container of claim 1 , wherein the second side includes a ridge aligned along the periphery of the opening claim 1 , and the closure element is a cap removably coupled to the ridge.8. The container of claim 1 , wherein the port is circular and the opening is rectangular.9. The container of claim 1 , wherein a medium resides within the chamber.10. The container of claim 1 , wherein the chamber includes rounded corner walls.11. The container of claim 1 , wherein the chamber has a curvilinear peripheral wall.12. A container in the shape of a rectangular cuboid comprising:a transparent first panel;a transparent second panel opposing the first panel;a first side;a second side opposing the first side;a third side;a fourth side opposing the third side;wherein each of the sides joins the first panel and the second panel;a chamber ...

ROTATABLE CARTRIDGE WITH A METERING CHAMBER FOR ANALYZING A BIOLOGICAL SAMPLE

An automatic analyzer cartridge, spinnable around a rotational axis, has aliquoting and metering chambers, a connecting duct there between, and a vent connected to the metering chamber and nearer to the rotational axis than the metering chamber. The metering chamber has side walls that taper away from a central region. Capillary action next to the side walls is greater than in the central region. A circular arc about the rotational axis passes through a duct entrance in the aliquoting chamber and a duct exit in the metering chamber. The cartridge has a downstream fluidic element which is part of a fluidic structure for processing a biological sample into the processed biological sample. A valve connects the metering chamber to the fluidic element, which is fluidically connected to the fluidic structure. The fluidic structure receives the biological sample and has a measurement structure for enabling measurement of the processed biological sample. 1. A method of aliquoting a fluid using a cartridge , wherein the cartridge is operable for being spun around a rotational axis , wherein the cartridge comprises:an aliquoting chamber;a metering chamber, wherein the metering chamber has side wall regions and a central region, wherein the side wall regions are narrower than the central region in a cross sectional view of the metering chamber, wherein capillary action next to the side wall regions of the metering chamber is greater than in the central region of the metering chamber;a connecting duct for connecting the metering chamber with the aliquoting chamber, wherein the connecting duct comprises a duct entrance in the aliquoting chamber, wherein the connecting duct further comprises a duct exit in the metering chamber, wherein a circular arc about the rotational axis passes through both the duct entrance and the duct exit;a vent, wherein the vent is connected to the metering chamber, wherein the vent is nearer to the rotational axis than the metering chamber; anda valve ...

ROTARY PLATFORM FOR CELL LYSING AND PURIFICATION AND METHOD OF USE

Magnetic beads having cell components of interest are translated between a sequence of processing wells in a tray without need for pipetting. The circular tray contains one or more sequences of wells each interconnected by a respective channel. The tray is rotated about a central axis and a magnet, an agitator, and a heater provided external to the tray enable magnetic bead translation, mixing, and incubation, respectively. The magnet proximate a well forms a cluster of beads. Manipulation of the tray in rotation and elevation results in translation of the cluster from one well, through a channel, and into an adjacent well. The well containing a cluster may be rotationally positioned in front of the agitator, the agitator extended into contact with the well, followed by mechanical agitation. The heater, disposed beneath the tray, may accept a well lowered thereto for selective heating. 1. A rotary platform for cell lysing , purification , and elution , comprising:a substantially circular tray having an upper surface, a lower surface, a thickness between the upper surface and the lower surface, an axis of symmetry orthogonal to the upper and lower surfaces, and an outer radial edge;a sequence of plural wells, each well having an outer surface extending from the lower surface of the tray about a periphery of the tray and having an interior volume and an opening in the tray upper surface, the opening extending into the respective well interior volume, the wells of the sequence of plural wells being disposed in an arc equidistant from the axis of symmetry; anda channel formed in the tray upper surface intermediate each pair of adjacent ones of the plural wells in the respective sequence, each channel having a depth less than the thickness and extending between the openings of the adjacent ones of the plural wells.2. The rotary platform of claim 1 , wherein each well outer surface is frustoconical.3. The rotary platform of claim 1 , wherein each well extends ...

MICROFLUIDIC STRUCTURE, MICROFLUIDIC DEVICE HAVING THE SAME AND METHOD OF CONTROLLING THE MICROFLUIDIC DEVICE

A microfluidic structure in which a plurality of chambers arranged at different positions are connected in parallel and into which a fixed amount of fluid may be efficiently distributed without using a separate driving source, and a microfluidic device having the same. The microfluidic device includes a platform having a center of rotation and including at least one microfluidic structure. The microfluidic structure includes a sample supply chamber configured to accommodate a sample, a plurality of first chambers arranged in a circumferential direction of the platform at different distances from the center of rotation of the platform, and a plurality of siphon channels, each of the siphon channels being connected to a corresponding one of the first chambers. 1. (canceled)2. A test device , comprising: an accommodating chamber configured to accommodate a fluid;', 'a metering chamber configured to meter an amount of the fluid;, 'a microfluidic device including a platform having a channel fluidly connecting the accommodating chamber, the metering chamber and the reaction chamber to each other;', 'a rotary drive unit configured to rotate the platform of the microfluidic device;', 'a magnet module movable in a radial direction of the platform; and', 'a controller to control the rotary drive unit and the magnet module., 'a reaction chamber configured to have a chromatographic reaction occur therein using the fluid metered in the metering chamber and introduced therein; and'}3. The test device according to claim 2 , wherein the controller is configured to rotate the platform and transfer the fluid accommodated in the accommodating chamber to the metering chamber claim 2 , and repeat intervals comprising increasing rotational speed of the platform and stopping rotation thereof claim 2 , such that the fluid flows into the reaction chamber4. The test device according to claim 3 , wherein the rotation is in a single direction.5. The test device according to claim 2 , wherein ...

Fluidic devices for chromatographic separation and methods of making and using the same

Disclosed herein are embodiments of fluidic devices that can be used to detect the presence (or absence) of analytes in samples by providing separate and distinct chromatographic signals for particular analytes. The fluidic devices described herein are highly sensitive and user-friendly. Methods of making and using the disclosed fluidic devices also are disclosed herein.

Device And Method For Isolating A Target From A Biological Sample

A device and a method for isolating a target from a biological sample are provided. The target is bound to solid phase substrate to form target bound solid phase substrate. The device includes a lower plate with an upper surface having a plurality of regions. The biological sample is receivable on a first of the regions. An upper plate has a lower surface directed to the upper surface of the lower plate. A force is positioned adjacent the upper plate and attracts the target bound solid phase substrate toward the lower surface of the upper plate. At least one of the upper plate and the lower plate is movable from a first position wherein the target bound solid phase substrate in the biological sample are drawn to the lower surface of the upper plate and a second position wherein the target bound solid phase substrate are isolated from the biological sample. 2. The device of wherein the regions of the lower plate are hydrophilic.3. The device of wherein portions of the upper surface of the outside of the regions of the lower plate are hydrophobic.4. The device of wherein the lower surface of the upper plate is hydrophobic.5. The device of wherein the upper plate is axially movable between the first and second positions.6. The device of wherein the upper plate is rotatable between the first and second positions.7. The device of wherein the upper surface of the lower plate and lower surface of the upper surface are spaced by a predetermined distance.8. A method for isolating a target from a biological sample claim 1 , the target being bound to a solid phase substrate to form a target-bound solid phase substrate claim 1 , comprising the steps of:providing the biological sample at a first region of a surface of a first plate;depositing a fluid on a second region of the surface of the first plate, the second region being spaced from the first region such that the fluid is spaced from the biological sample along the surface of the first plate;positioning a second plate in ...

Rotatable cartridge for processing and analyzing a biological sample

An automatic analyzer cartridge, spinnable about a rotational axis, has fluid and aliquoting chambers, a metering chamber connected to a vent that is nearer to the rotational axis than the metering chamber, first and second ducts connecting the fluid and aliquoting chambers, and the metering and aliquoting chambers, respectively. Metering chamber side walls taper away from a central region, wherein capillary action next to the walls is greater than in the central region. Fluid flows to the metering chamber using capillary action via the second duct that has an entrance and exit in the aliquoting and metering chambers, respectively; the exit being closer to the rotational axis than the entrance. A downstream fluidic element connects to the metering chamber via a valve. A fluidic structure receives and processes a biological sample into the processed biological sample and has a measurement structure that enables measurement of the processed biological sample.

ROTATABLE CARTRIDGE FOR ANALYZING A BIOLOGICAL SAMPLE

An automatic analyzer cartridge spinnable around a rotational axis has a fluid chamber for receiving a fluid, an aliquoting chamber, a duct connecting the fluid chamber and the aliquoting chamber, a downstream fluidic element, a siphon for siphoning the fluid from the aliquoting chamber to the downstream fluidic element, a fluidic structure for processing a biological sample into the processed biological sample, and a measurement structure for enabling measurement of the processed biological sample. The siphon has a siphon entrance in the aliquoting chamber and a siphon exit in the downstream fluidic element, wherein the siphon has a bend, wherein the bend is the portion of the siphon closest to the rotational axis, wherein the siphon entrance starts at the bend, wherein the siphon entrance extends to a lower portion of the aliquoting chamber. This enables multiple aliquots of fluid to be removed from the aliquoting chamber by the siphon. 2. The method of claim 1 , wherein the cartridge further comprises a reservoir filled with the fluid claim 1 , wherein the reservoir is configured for being opened and for transferring the fluid to the fluid chamber.3. The method of claim 1 , wherein the siphon is operable for causing fluid to flow to the downstream fluidic element using capillary action.4. A cartridge for an automatic analyzer claim 1 , wherein the cartridge is operable for being spun around a rotational axis claim 1 , wherein the cartridge comprises:a fluid chamber for receiving a fluid;an aliquoting chamber;a duct connecting the fluid chamber and the aliquoting chamber;a downstream fluidic element;a siphon for siphoning the fluid from the aliquoting chamber to the downstream fluidic element, wherein the siphon comprises a siphon entrance in the aliquoting chamber, wherein the siphon further comprises a siphon exit in the downstream fluidic element, wherein the siphon comprises a bend, wherein the bend is the portion of the siphon closest to the rotational axis, ...

Cartridge with a rotatable lid

An automatic analyzer cartridge, spinnable around a rotational axis, has a support structure with a front face perpendicular to the rotational axis, a fluidic structure for processing a biological sample into the processed biological sample, a measurement structure with at least one detection zone on the front face, and a rotatable lid covering the front face. The rotatable lid is rotatable about the rotational axis relative to the support structure from a first position relative to the support structure to a second position relative to the support structure. The rotatable lid has a sample inlet opening and a detection zone opening. In the first position, a sample inlet is aligned with the sample inlet opening and the measurement structure is covered by the rotatable lid. In the second position, the sample inlet is covered by the rotatable lid and the measurement structure is aligned with the detection zone opening.

A NON-INVASIVE PAPER BASED DEVICE FOR PREGNANCY DETECTION IN CATTLE AND BUFFALOES

The present invention discloses a kit and a novel method of extraction of a progesterone metabolite from feces/dung sample. The present invention also discloses a paper-based microfluidic device for detection of the progesterone metabolite for early determination of pregnancy in cattle and buffaloes. The invention further discloses antibodies developed against said metabolite, method of extraction, methods for fabricating the paper-based microfluidic devices and methods for inexpensive, rapid and non-invasive determination of pregnancy in early stages in cattle and buffaloes. 2100. The microfluidic device as claimed in claim 1 , wherein said device is a single-layer paper device () having three circular zones configured to receive a biological sample and a reagent claim 1 , comprising:{'b': '101', 'a. a test zone () comprising immobilized 5α-pregnan-3α-ol-20-one antigen for colorimetric detection of 5α-pregnan-3α-ol-20-one;'}{'b': '102', 'b. a positive control zone () comprising immobilized 5α-pregnan-3α-ol-20-one antigen for colorimetric detection of 5α-pregnan-3α-ol-20-one;'}{'b': '103', 'c. a negative control zone (); and'}{'b': '104', 'd. a wax pattern () on the paper layer, wherein the wax pattern outlines the test zone, the positive control zone and the negative control zone to create a hydrophobic barrier.'}3200. The microfluidic device as claimed in claim 1 , wherein said device is a multi-layer device () comprising:{'b': '300', 'a. a first layer () configured for receiving a biological sample and reagents;'}{'b': '400', 'b. a second layer () configured for delivery of the biological sample and the reagents to a reaction zone;'}{'b': '500', 'c. a third layer () configured for delivery of the biological sample to the reaction zone; and'}{'b': '600', 'd. a fourth layer () configured for colorimetric detection of 5α-pregnan-3α-ol-20-one.'}4300200. The microfluidic device as claimed in claim 3 , wherein the first layer () of the multilayer device () comprises:{' ...

THIN, SOFT, SKIN-MOUNTED MICROFLUIDIC NETWORKS FOR DETECTION AND ANALYSIS OF TARGETS OF INTEREST IN SWEAT

Provided herein are flexible, microfluidic epidermal systems and methods useful in the analysis of biofluids for biomarkers corresponding to a variety of conditions and methods of use. The provided systems configured to create conformal contact with the skin to allow for medical testing or screening, either in situ or later external laboratory testing. The described devices and methods may be used for cystic fibrosis screening, glucose monitoring, drug and/or alcohol testing, creatinine monitoring, urea monitoring, pH measurement and dialysis treatment efficacy testing. 1. The method of claim 2 ,wherein said one or more biomarkers are for i) monitoring or screening for cystic fibrosis, ii) monitoring drug or alcohol consumption, iii) monitoring dialysis efficacy, iv) monitoring glucose levels, v) monitoring creatinine levels, vi) monitoring urea levels, vii) monitoring pH or any combination thereof.2. The method of claim 3 , further comprising at least one of:measuring the volume of said sweat collected or the flow rate of said sweat from said subject; andmonitoring a health condition of said subject based on said one or more biomarkers in said sweat.3. A method for the detection of a biomarker in sweat comprising: a flexible substrate;', 'a sweat inlet embedded in or supported by said flexible substrate; and', 'at least one reservoir chamber fluidically connected to the sweat inlet;, 'providing an epidermal microfluidic device to a skin of a subject, wherein conformal contact is established thereby providing fluidic communication between said device and said skin, said device comprisingcollecting said sweat from said subject in said device; andidentifying or quantifying the amount of one or more biomarkers in said sweat, wherein each of said one or more biomarkers is identified or quantified in a unique reservoir chamber.4. The method of claim 2 , wherein said device further comprises an analyzer integrated with or fluidically connected to the at least one ...

SAMPLE ANALYSIS METHODS AND APPARATUSES AND DYNAMIC VALVE OPERATION METHODS

Disclosed are sample analysis apparatus and methods. A sample analysis apparatus includes a first unit that rotates a microfluidic apparatus including: a chamber having a space accommodating a sample, a channel that provides a path through which the sample flows; and a valve that selectively opens and closes the channel, a valve driver that supplies energy, used to operate the valve, to the valve in a state of being separated from the microfluidic apparatus, a third unit that rotates the valve driver with respect to a common rotation axis with a rotation axis of the microfluidic apparatus and the third unit, and a control unit that controls the first and third units and the valve driver to supply energy to the valve while the microfluidic apparatus and the valve driver are being rotated at the same rotation speed. 1. A sample analysis apparatus comprising:a first unit that rotates a microfluidic apparatus comprising a chamber with a space for accommodating a sample; a channel that provides a path through which the sample flows; and a valve that selectively opens and closes the channel;a valve driver that supplies energy, used to operate the valve, to the valve in a state of being separated from the microfluidic apparatus;a third unit that rotates the valve driver with respect to a common rotation axis with a rotation axis of the microfluidic apparatus; anda control unit that controls the first and third units and the valve driver to supply energy to the valve while the microfluidic apparatus and the valve driver are being rotated at the same rotation speed.2. The sample analysis apparatus of claim 1 , further comprising a second unit that moves the valve driver in a radial direction of the microfluidic apparatus claim 1 ,wherein the control unit controls the first, second, and third units so that positions in the radial direction and angular positions of the valve driver and the valve are aligned with each other.3. The sample analysis apparatus of claim 2 , wherein ...

DEVICE AND METHOD FOR HANDLING LIQUID

A liquid handling device having an axis of rotation about which the device can be rotated to drive liquid flow in the device. The device includes an upstream chamber comprising an outlet, a downstream chamber including a proximal portion radially inwards of a distal portion and including a first port disposed in the distal portion and a first conduit which connects the outlet of the upstream chamber to the first port of the downstream chamber. The first conduit extends radially inwards to a crest and radially outwards from the crest to the first port of the downstream chamber. A distance between the axis of rotation and the crest is greater than or equal to a distance between the axis of rotation and the outlet of the upstream chamber. 1. A liquid handling device having an axis of rotation about which the device can be rotated to drive liquid flow in the device , the device comprising:an upstream chamber comprising an outlet;a downstream chamber comprising a proximal portion radially inwards of a distal portion and comprising a first port disposed in the distal portion; anda first conduit which connects the outlet of the upstream chamber to the first port of the downstream chamber;wherein the first conduit extends radially inwards to a crest and radially outwards from the crest to the first port of the downstream chamber; andwherein a distance between the axis of rotation and the crest is greater than or equal to a distance between the axis of rotation and the outlet of the upstream chamber.2. A liquid handling device as claimed in claim 1 , where the first port is disposed in a radially-outermost aspect of the downstream chamber.3. A liquid handling device as claimed in claim 1 , wherein the first conduit extends radially outwards of the first port between the first port and the crest.4. A liquid handing device as claimed in claim 1 , wherein the upstream chamber comprises a metering portion and an overflow portion;wherein a wall of the upstream chamber extends ...

Cartridge, detection method, and detection device

The present invention provides a cartridge, a detection method, and a detection device capable of stabilizing the liquid level of a sample accommodated in a chamber in a predetermined state. A cartridge 20 , that is rotated around a rotating shaft 42 for detecting a target substance, is provided with a chamber 100 in which a sample containing a target substance is stored. The chamber 100 includes a first region 110 in which a sample is stored, a second region 120 disposed at a position closer to the rotating shaft 42 than the first region 110 , and a protrusion 130 protruding from a position between the first region 110 and the second region 120 to the inner side of the chamber 100.

Point of Care Concentration Analyzer

An analyzer system includes a cartridge configured to receive a sample. The cartridge has a plurality of chambers for isolating a target analyte of the sample and collecting a quantity of a first label that is proportional to a quantity of the target analyte in the sample. The system includes an analyzer with a first electromagnetic radiation source a first detector and a controller. The first electromagnetic radiation source is configured to provide electromagnetic radiation to form an interrogation space within a detection chamber of the cartridge. The first detector is configured to detect electromagnetic radiation emitted in the interrogation space by the first label if the first label is present in the interrogation space. The controller is configured to identify the presence of the target analyte in the sample based on electromagnetic radiation detected by the first detector. 1. An analyzer system for measuring a concentration of a target analyte in a sample , the analyzer system comprising:a motor;a dock coupled to the motor so as to be rotated by actuation of the motor; an inlet chamber,', 'a mixing chamber downstream of the inlet chamber and configured to mix at least a portion of the sample so as to bind the target analyte with the first label, and', 'a wash chamber downstream of the mixing chamber and connected to the mixing chamber by a channel that extends radially inward from the mixing chamber so as to impede flow of the sample into the wash chamber during a mixing process that is carried out in the mixing chamber;, 'a cartridge held in the dock and including a fluid system configured to receive a sample, isolate a target analyte of the sample, and collect a quantity of a first label that is proportional to a quantity of the target analyte in the sample, the fluid system includinga first electromagnetic radiation source configured to provide electromagnetic radiation to form an interrogation space within a detection chamber of the cartridge;a first ...

DEVICE FOR DETERMINING THE PRESENCE OF A BACTERIOLOGICAL CONTAMINATION IN A FLUID

A device for determining contamination of a fluid by microorganisms has a housing with an internal volume, a cover closing the housing, a fluid inlet port, at least one filtration member, at least one nutrient layer including a composition of a microbiological culture medium, characterized in that the device includes a fluid outlet port, and in that the cover has an inner surface in the internal volume extending radially about the fluid inlet port up to a peripheral edge of the cover, said inner surface being inclined and converging towards the fluid inlet port, and in that the bottom of the housing has a surface extending radially about the fluid outlet port up to the side wall of the housing, said inner surface being inclined and converging towards the fluid outlet port. 2. The device as claimed in claim 1 , in which the inclination of the inner surface of the cover is strictly greater than 4°.3. The device as claimed in claim 1 , in which the inclination of the surface of the bottom is strictly less than 0°.4. The device as claimed in claim 1 , in which the fluid inlet port projects into the internal volume of the housing relative to the inner surface of the cover.5. The device as claimed in claim 1 , in which the filtration member is arranged in the housing at a given distance h from the inlet port claim 1 , the given distance h being strictly greater than 1 mm.6. The device as claimed in claim 1 , in which the housing has a first circumferential bearing surface shaped to cooperate complementarily with an edge of the cover.7. The device as claimed in claim 1 , in which the housing has at least one second bearing surface extending in the internal volume and shaped to receive a peripheral portion of the filtration member claim 1 , said second bearing surface being shaped to cooperate with a portion of the cover such that the peripheral portion of the filtration member is clamped between the cover and the housing.8. The device as claimed in claim 1 , in which the ...

MICROFLUIDIC DEVICE AND ARRAY DISK

The invention relates to microfluidic devices and array disks for “one-pot” isolated chemical reactions. The array disks comprise a plurality of sectors in which each sector comprises one microfluidic device. The microfluidic devices comprise a fluid delivery channel and an array of wells wherein the fluid delivery channel delivery fluid into the wells in a serpentine arrangement. In some embodiments, the fluid delivery channel is directly above the array of wells. In other embodiments, the fluid delivery channel is offset from the array of wells so that side channels branching from the fluid delivery channel delivers fluid into the wells. The well of the microfluidic device comprises a gas-permeable membrane that forms the floor, well, or at least a portion of the floor or wall of the well. In preferred embodiments, the well is cylindrical. 1. A microfluidic device comprising:a fluid delivery channel; a hydrophilic coating;', 'a gas-permeable membrane;', 'a top opening; and', 'a bottom opening, wherein the bottom opening is sealed by the gas-permeable membrane to form the floor of each well; and, 'an array of wells arranged to follow the serpentine flow of a fluid delivery channel, wherein each well of the array of wells comprisesa reservoir,wherein the top opening of each well meets fluid delivery channel; the reservoir is below gas-permeable membrane; and the fluid delivery channel directs a fluid into each well in a serpentine order whereby an entire column of wells is filled before wells in the next column are filled.2. The microfluidic device of claims 1 , wherein the aspect ratio of the device is at least 1.3. The microfluidic device of claim 1 , wherein each wells is cylindrical.4. The microfluidic device of claim 1 , wherein each well is at least 100 μm tall thereby providing a long optical pathway length for optical detection of the contents of each well.5. The microfluidic device of claim 4 , wherein optical detection comprises colorimetic or ...

Characterization of reaction variables

A microscale method for the characterization of one or more reaction variables that influence the formation or dissociation of an affinity complex comprising a ligand and a binder, which have mutual affinity for each other. The method is characterized in comprising the steps of: (i) providing a microfluidic device comprising a microchannel structures that are under a common flow control, each microchannel structure comprising a reaction microactivity; (ii) performing essentially in parallel an experiment in each of two or more of the plurality of microchannel structures, the experiment in these two or more microchannel structures comprising either a) formation of an immobilized form of the complex and retaining under flow conditions said form within the reaction microactivity, or b) dissociating, preferably under flow condition, an immobilized form of the complex which has been included in the microfluidic device provided in step (i), at least one reaction variable varies or is uncharacterized for said two or more microchannel structures while the remaining reaction variables are kept essentially constant; (iii) measuring the presentation of the complex in said reaction microactivity in said two or more microchannel structures; and (iv) characterizing said one or more reaction variables based on the values for presentation obtained in step (iii).

APPARATUS AND METHOD FOR DIRECTING A LIQUID THROUGH A POROUS MEDIUM

An apparatus for directing a liquid through a porous medium includes a fluidic module rotatable about a center of rotation and including a fluid chamber and an inflow structure. A porous medium is disposed in the fluid chamber to allow centrifugal force-effected flow of the liquid impinging on a radially inner portion of the porous medium, to a radially outer portion of the porous medium. The porous medium is laterally at least partially spaced apart from chamber walls of the fluid chamber with respect to the flow, so that a fluid connection exists between the radially inner portion of the porous medium and the radially outer portion of the porous medium outside the porous medium. The inflow structure is configured to limit a centrifugal force-effected inflow of the liquid to the radially inner portion of the porous medium to a first flow rate, wherein a ratio of the first flow rate to a maximum possible flow rate through the porous medium is not greater than two. 1. An apparatus for directing a liquid through a porous medium , comprising:a fluidic module rotatable about a center of rotation and comprising a fluid chamber and an inflow structure,a porous medium disposed in the fluid chamber to allow centrifugal force-effected flow of the liquid impinging on a radially inner portion of the porous medium to a radially outer portion of the porous medium, wherein the porous medium is laterally at least partially spaced apart from chamber walls of the fluid chamber with respect to the flow, so that a fluid connection exists between the radially inner portion of the porous medium and the radially outer portion of the porous medium outside the porous medium, which represents a bypass for a liquid flow not through the porous medium between the chamber walls and the porous medium,wherein the inflow structure is configured to limit a centrifugal force-effected inflow of the liquid to the radially inner portion of the porous medium to a first flow rate,wherein a ratio of the ...

ANALYSIS INSTRUMENT

An apparatus for moving a sample holder on a platform from a loading position, where the sample holder can be removed from the platform , to a locked position, where the sample holder is securely held, wherein the sample holder rests on wheels within a recessed portion on the platform , wherein the apparatus is configured such that movement from the loading position to the locked position causes a vertical clamping means to lower down on top of the sample holder , and a horizontal clamping means to be pressed to the outer periphery of the sample holder , and such that that movement from the locked position to the loading position causes the vertical clamping means to raise above the sample holder , and the horizontal clamping means to be moved away from the outer periphery of the sample holder 1. An apparatus for moving a sample holder on a platform from a loading position , where the sample holder can be removed from the platform , to a locked position , where the sample holder is securely held ,wherein the sample holder rests on wheels within a recessed portion on the platform,wherein the apparatus is configured such that movement of the platform from the loading position to the locked position causes a vertical clamping means to lower down on top of the sample holder, and a horizontal clamping means to be pressed to the outer periphery of the sample holder, and such that that movement of the platform from the locked position to the loading position causes the vertical clamping means to raise above the sample holder, and the horizontal clamping means to be moved away from the outer periphery of the sample holder.2. An apparatus according to claim 1 , wherein the vertical clamping means comprises a platform lid; optionally wherein in the locked position claim 1 , the platform lid lies parallel to the platform.3. An apparatus according to claim 2 , wherein the platform lid comprises a first end which is hingedly attached to the platform claim 2 , and a second end ...

A MICRO-FLUIDIC DEVICE FOR CONCENTRATION OF PARTICLES

A microfluidic device for concentrating particles contained in a fluid sample comprises a substrate () having a surface where at least one microfluidic arrangement (M) is defined, which comprises: —a loading chamber (), for loading the fluid sample into the at least one microfluidic arrangement (M); —a plurality of microchannels (), which have respective inlet ends connected to the loading chamber (); and—a covering element (), which is substantially impermeable to the fluid sample and extends at least partially over the plurality of microchannels (). The loading chamber () and the microchannels () extend substantially according to a plane identified by the substrate (), and the microchannels () are partially delimited, in particular at an accumulation region thereof (CA) generally opposite to the respective inlet ends, by filtering means () permeable at least to air, the filtering means () being configured for withholding within each microchannel () any possible particles that may be present in the fluid sample. 1. A microfluidic device for concentrating particles contained in a fluid sample , comprising a substrate having a surface at which at least one microfluidic arrangement is defined , which comprises:a loading chamber, for loading the fluid sample into the at least one microfluidic arrangement;a plurality of microchannels, which have respective inlet ends connected to the loading chamber, in particular with a fluidic connection in parallel to said chamber;a covering element, which is substantially impermeable to the fluid sample and extends at least partially over the plurality of microchannels,wherein the loading chamber and the microchannels extend substantially according to a plane identified by the substrate,wherein the microchannels are partially delimited, at least at an accumulation region thereof generally opposite to the respective inlet ends, by filtering means permeable at least to air, the filtering means being configured for withholding within ...

DEVICE FOR STORAGE AND DISPENSING OF REAGENTS

A reagent storage and dispensing apparatus comprises a housing, storage wells disposed within an internal volume of the housing, and a valve. The housing comprises a shared outlet chamber. The wells can be selected to be in fluid communication with the shared outlet channel through individual outlet channels. The valve is coupled to the housing and is rotated to select a well to be in fluid communication with the shared outlet channel while preventing the remaining wells from being in fluid communication. As the valve is rotated, the wells and their individual outlet channels remain stationary. To dispense fluid from or draw fluid into the selected well, positive or negative pressure, respectively, is introduced into the internal volume. Pressurization of the internal volume also pressurizes the plurality of wells, though the valve allows only fluid flow for the selected well. 1. An apparatus for the storage and dispensing of one or more reagents or fluids , the apparatus comprising:a housing having an internal volume and comprising an outlet channel and an inlet port configured to allow the internal volume to be pressurized;a plurality of storage wells disposed within the internal volume of the housing, the plurality of storage wells being selectively in fluid communication with the outlet channel; anda valve coupled to the housing, the valve being rotatable to select an individual storage well to be in fluid communication with the outlet channel while preventing the remaining storage wells from being in fluid communication with the outlet channel,wherein pressurizing the internal volume of the housing causes fluid to be drawn into or egress from the selected individual storage well.2. The apparatus of claim 1 , wherein pressurizing the internal volume of the housing comprises introducing one or more of negative pressure or positive pressure to the internal volume.3. The apparatus of claim 2 , wherein the introduction of positive pressure into the internal volume ...

MULTI-CHAMBERED ASSAY DEVICES AND ASSOCIATED METHODS, SYSTEMS AND APPARATUSES THEREOF FOR DETECTION OF ANALYTES

Accordingly, in some embodiments of the disclosure, a multi-chambered assay device is provided, which is configured for arrangement on a disc, as well as configured to process an individual sample. A plurality of such assay devices can be arranged along a periphery of the disc at a distance/radius from the center, such that a plurality of individual samples can be processed, e.g., one per assay device. In addition, in an arrangement that a plurality of assay devices are used, they can be spaced apart such that they balance the disc during rotation (which can be with samples contained in one or more of the assay devices, a plurality, a majority, or all of the assay devices). 1. An assay device configured for arrangement on a disc , and configured to process an individual sample , the assay device positioned along a periphery of the disc at a predetermined radius in a spaced apart arrangement , the assay device comprising:a plurality of peripheral chambers each configured to receive one or more fluids via a respective inlet area;a resuspension chamber including a scaffold for at least one of drying and retaining at least one reagent; anda main chamber having a mixing area and at least one bead therein2. The device of claim 1 , wherein the plurality of chambers comprise at least one of a first peripheral chamber claim 1 , a second peripheral chamber claim 1 , and a third peripheral chamber.3. The device of claim 1 , wherein each peripheral chamber includes a corresponding inlet area.4. The device of claim 5 , wherein each inlet area is configured to flow or otherwise transfer a fluid to a respective peripheral chamber via a microfluidic channel.5. The device of claim 1 , wherein the plurality of peripheral chambers comprise at least two of:a first peripheral chamber having an associated first inlet area, the first inlet area in fluid communication with the first peripheral chamber via a first microchannel, wherein fluid received in the first inlet area flows into the ...

MICROPLATES FOR MAGNETIC 3D CULTURE

Devices for magnetic 3d culture are described including magnetic lids/bases for single Petri plates and adjustable height cap for same. Similar devices for multi-magnet culture plates wherein multiwell plates have all adjacent magnets orientated in the opposite polarity, and methods of making same. 1. A magnetic culture plate for three-dimensional (3D) cell culturing , comprising:a) a standard ANSI-SLAS microtiter plate comprising a plurality of wells in an array and having a length of 127.76 mm±0.5 mm and a width of 85.48 mm±0.5 mm,b) a cap sized to cover said standard ANSI-SLAS microtiter plate, said cap having lip around a circumference thereof and a plurality of magnets in an array, each magnet affixed to a depression in said cap or on a post on said cap, such that each well has a single magnet over or under said well when said cap is fitted in place over or under said standard ANSI-SLAS microtiter plate and such that each adjacent magnet is affixed in opposite polarity, wherein said cap is sized to fit both over and under said standard ANSI-SLAS microtiter plate, and wherein each single magnet of the plurality of magnets protrudes inside a well when said cap is fitted in place over said standard ANSI-SLAS microtiter plate so that the magnet or an intervening cover does not touch a culture media inside said well and wherein the cap holds the magnets at a fixed distance of 0.2 to 10 mm from said culture media inside said well.2. The magnetic culture plate of claim 1 , wherein said magnets are exchangeably affixed to the depression or post on the cap.3. The magnetic culture plate of claim 1 , said standard ANSI-SLAS microtiter having 6 wells and said cap having 6 magnets of 20-100 lbs pull force claim 1 , 10000-15000 Gauss Brmax and 30-60 MGOe Bhmax.4. The magnetic culture plate of claim 1 , said standard ANSI-SLAS microtiter having 12 wells and said cap having 12 magnets of 2-20 lbs pull force claim 1 , 10000-15000 Gauss Brmax and 30-60 MGOe Bhmax.5. The magnetic ...

MEASUREMENT OF AN ANLYTE WITH A CARTRIDGE

A method of performing an optical measurement of an analyte in a processed biological sample using a cartridge is provided. The cartridge is operable for being spun around a rotational axis. The method comprises: placing the biological sample into a sample inlet; controlling the rotational rate of the cartridge to process a biological sample into the processed biological sample using a fluidic structure; controlling the rotational rate of the cartridge to allow the processed biological sample to flow from the measurement structure inlet to an absorbent structure via a chromatographic membrane, and performing an optical measurement of a detection zone on the chromatographic membrane with an optical instrument. An inlet air baffle reduces evaporation of the processed biological sample from the chromatographic membrane during rotation of the cartridge. 2. The method of claim 1 , wherein the method further comprises:placing buffer solution at the measurement structure inlet after controlling the rotational rate of the cartridge to allow the processed biological sample to flow from the measurement structure inlet to the absorbent structure via the chromatographic membrane; andwashing the chromatographic membrane by controlling the rotational rate of the cartridge to allow the buffer solution to flow from the measurement structure inlet to the absorbent structure via the chromatographic membrane before performing the optical measurement.3. The method of claim 1 , wherein the measurement structure comprises at least one air pocket adjacent to the chromatographic membrane claim 1 , wherein the at least one air pocket is covered by the front face parallel to the rotational axis.4. The method of claim 1 , wherein along a circumferential path across the detection zone the inlet air baffle has a first air baffle edge and a second air baffle edge where the inlet air baffle meets the front face.5. The method of claim 4 , wherein along the rotational axis the first air baffle edge ...

APPARATUSES WITH PRE-PACKAGED REAGENTS

Apparatuses with pre-packaged reagents comprise a reagent package and a protrusion member. More particularly, the reagent package comprises a base and a cover layer, in which the base further comprises at least one pocket. Each pocket is configured to store a reagent, and the cover layer is configured on the at least one pocket to seal the at least one pocket. The protrusion member further comprises an opening and is configured at an injection site, in which the injection site is on each storage chamber disposed on a microfluidic disc. Furthermore, the configuration of the pockets on the base is in accordance with the configuration of the protrusion members. Therefore, the protrusion members would punch through the pockets once the protrusion members are aligned and combined to the pockets. And the following rotation of the microfluidic disc would generate centrifugal force and sequentially release reagents into storage chambers. 1. An apparatus with pre-packaged reagents , comprising: a base comprising at least one pocket, wherein the at least one pocket each is configured to store a reagent; and', 'a cover layer disposed on the at least one pocket, wherein the cover layer is configured to seal the at least one pocket; and, 'a reagent package, comprisingat least one protrusion member having an opening, wherein the at least one protrusion member is disposed at an injection site, and wherein the injection site is located at each storage chamber configured on each microfluidic channel disposed on a microfluidic disc;wherein the position of the at least one pocket on the base is in accordance with the position of the at least one protrusion member;wherein the at least one protrusion member is configured to punch through the cover layer when the at least one pocket is aligned and combined with the at least one protrusion member and to sequentially release the reagent in the at least one pocket into the storage chamber by the centrifugal force generated by rotation of ...

Gas-based microfluidic devices and operating methods thereof

Gas-based microfluidic devices and operating methods of gas-based microfluidic devices are provided. The gas-based microfluidic devices comprise a drive module and a microfluidic platform, in which the microfluidic platform further comprises a microfluidic element having an injection chamber, a process chamber, an air chamber, an overflow channel, a barrier, and at least one detection chamber. Gases in the air chamber enable solutions to move toward the direction opposite to the centrifugal force applied by the drive module. Accordingly, the operating methods utilize the gases compressed in the air chamber to move solutions to difference components in the microfluidic element.

Sample preparation device

A sample preparation device, preferably for sterility testing, comprising a manifold including one or more receptacles for filtration units and at least one inlet and/or outlet port. The receptacle(s) is/are respectively provided with one or more connectors for establishing a fluid connection with mating ports of the filtration units and media containers/vials upon insertion of the same into the respective receptacles. The connectors are in fluid communication with the inlet and outlet port(s) via channels defined in the manifold to allow a desired fluid transfer through the manifold.

Method for producing microcarriers and for performing biological assays

The method according to the invention consists in providing a wafer having a bottom layer, a top first sacrificial layer and an insulating layer, structuring the first sacrificial layer to form a three dimensional structure onto which a first structural layer is deposited to define a corresponding three dimensional structure on the bottom surface of the first structural layer. The method consists also in forming a second three dimensional structure on the upper surface of the first structural layer.

HIGH-THROUGHPUT PARTICLE CAPTURE AND ANALYSIS

Microfluidic systems and methods are described for capturing magnetic target entities bound to one or more magnetic beads. The systems include a well array device that includes a substrate with a surface that has a plurality of wells arranged in one or more arrays on the surface. A first array of wells is arranged adjacent to a first location on the surface. A second and subsequent arrays, if present, are arranged sequentially on the surface at second and subsequent locations. When a liquid sample is added onto the substrate and caused to flow, the liquid sample will flow across the first array first and then flow across the second and subsequent arrays in sequential order. The wells in the first array each have a size that permits entry of only one target entity into the well and each well in the first array has approximately the same size. 1. A micro-well array device for capturing target entities that are , or are made to be , magnetic , the device comprising:a substrate including a surface comprising a plurality of micro-wells arranged in one or more arrays on the surface;wherein a first array of micro-wells is arranged at a first location on the surface;wherein second and subsequent arrays, if present, are arranged sequentially on the surface at second and subsequent locations, wherein when a liquid sample is added onto the substrate and caused to flow, the liquid sample will flow across the first array first and then flow across the second and subsequent arrays in sequential order;wherein micro-wells in the first array each have a size that permits entry of only one target entity into the micro-well and wherein each micro-well in the first array has approximately the same size;wherein micro-wells in the second and subsequent arrays, if present, each have a size that is at least ten percent larger than the size of the micro-wells in the previously adjacent array and wherein each micro-well in a given subsequent array has approximately the same size; andwherein ...