УСТРОЙСТВО ДЛЯ СМЕШИВАНИЯ ВЕЩЕСТВ И СПОСОБ СМЕШИВАНИЯ ВЕЩЕСТВ

Изобретение относится к смешиванию веществ и может использоваться в биотехнологии и химии. Устройство для смешивания вещества включает в себя: два или более проточных каналов (11, 12, 13), в которых формируются отверстия (111, 121, 131), из которых выпускается наружу жидкость, колебательные устройства (112, 122, 132), которые формируют капли жидкости, выпускаемые из каждого отверстия (111, 121, 131) за счет колебаний, по меньшей мере, части проточных каналов, где находятся отверстия (111, 121, 131), на заданной частоте колебаний, и выпускают капли жидкости; а также средства, для того чтобы вызвать столкновение друг с другом капель (A, B, C) жидкости, выпускаемых из отверстий (111, 121, 131) проточных каналов (11, 12, 13). Технический результат состоит в обеспечении равномерности смешивания определенного количества микроэлементов, а также смешивания мельчайших частиц. 3 н. и 15 з.п. ф-лы, 15 ил.

Dispensiergerät und entsprechendes Dispensierverfahren

Die Erfindung betrifft ein Dispensiergerät zum Dispensieren von Mikrotropfen (1), mit einem Mikrodispenser (2) mit einer Austrittsdüse (10) zum Dispensieren der flüssigen Mikrotropfen (1), wobei die flüssigen Mikrotropfen (1) jeweils eine Probe in einer wässrigen Lösung (8) enthalten, und mit einer Probenaufnahme (3, 4) zur Aufnahme der von dem Mikrodispenser (2) dispensierten Mikrotropfen (1), insbesondere als Aufnahmenapf (3) in einer Mikrotiterplatte (4). Die Erfindung sieht vor, dass die Probenaufnahme (3, 4) flüssiges Öl (14) enthält, so dass der Mikrodispenser (2) die Mikrotropfen (1) in das flüssige Öl (14) einspritzt. Weiterhin umfasst die Erfindung ein entsprechendes Dispensierverfahren.

Method and apparatus for manipulating particles

Detectors for microfluidic systems

A method for detecting and measuring samples in the form of liquid droplets or slugs in a carrier liquid comprises providing a microfluidic device with conduits and at least one detector, moving a carrier liquid containing the liquid droplets or slugs through the microfluidic device until a first droplet or slug comes into contact with the at least one detector, measuring at least one property of the liquid droplet or slug with the detector, removing the sample, flushing the detector with a flushing liquid and then repeating the detection sequence. A device for carrying out the method comprises a first conduit, 12 a detector 10 and a second conduit 14 in liquid communication with the first conduit.

Fluid handling system and method of manufacture

Acoustofluidic device

SYSTEM, PROCEDURE AND COMPUTER-IMPLEMENTED PROCESS FOR THE EXAMINATION OF COAGULATION IN LIQUID SAMPLES

PROCEDURE AND DEVICE FOR THE MIXING OF SMALL LIQUID QUANTITIES IN MICRO CAVITIES

DISPENSIERVERFAHREN

PROCEDURE AND DEVICE FOR THE ASSORTMENT OF MICROSCOPIC PARTICLES.

PROCEDURE FOR THE PRODUCTION OF INTEGRATIONS OF MULTILAYER MICRO FLUID CONSTRUCTION UNITS

PROCEDURE FOR THE MASS SPECTROMETRY

SYSTEM, PROCEDURE AND COMPUTER-IMPLEMENTED PROCESS FOR THE EXAMINATION OF COAGULATION IN LIQUID SAMPLES

SYSTEM, PROCEDURE AND COMPUTER-IMPLEMENTED PROCESS FOR THE EXAMINATION OF COAGULATION IN LIQUID SAMPLES

SYSTEM, PROCEDURE AND COMPUTER-IMPLEMENTED PROCESS FOR THE EXAMINATION OF COAGULATION IN LIQUID SAMPLES

SYSTEM, PROCEDURE AND COMPUTER-IMPLEMENTED PROCESS FOR THE EXAMINATION OF COAGULATION IN LIQUID SAMPLES

SYSTEM, PROCEDURE AND COMPUTER-IMPLEMENTED PROCESS FOR THE EXAMINATION OF COAGULATION IN LIQUID SAMPLES

SYSTEM, PROCEDURE AND COMPUTER-IMPLEMENTED PROCESS FOR THE EXAMINATION OF COAGULATION IN LIQUID SAMPLES

SYSTEM, PROCEDURE AND COMPUTER-IMPLEMENTED PROCESS FOR THE EXAMINATION OF COAGULATION IN LIQUID SAMPLES

SYSTEM, PROCEDURE AND COMPUTER-IMPLEMENTED PROCESS FOR THE EXAMINATION OF COAGULATION IN LIQUID SAMPLES

SYSTEM, PROCEDURE AND COMPUTER-IMPLEMENTED PROCESS FOR THE EXAMINATION OF COAGULATION IN LIQUID SAMPLES

SYSTEM, PROCEDURE AND COMPUTER-IMPLEMENTED PROCESS FOR THE EXAMINATION OF COAGULATION IN LIQUID SAMPLES

SYSTEM, PROCEDURE AND COMPUTER-IMPLEMENTED PROCESS FOR THE EXAMINATION OF COAGULATION IN LIQUID SAMPLES

DEVICES, SYSTEMS AND METHODS FOR EVALUATION OF HEMOSTASIS

Provided are devices, systems and methods for evaluation of hemostasis. Also provided are sound focusing assemblies.

DEVICES, SYSTEMS AND METHODS FOR EVALUATION OF HEMOSTASIS

Provided are devices, systems and methods for evaluation of hemostasis. Also provided are sound focusing assemblies. WO 2013/105986 PCT/US2012/025270 __~~ ~FG IF '~1y_ Bi il 116 114 112 110 FI.1 -ill.

Methods and apparata for precisely dispensing microvolumes of fluids

METHODS AND APPARATA FOR PRECISELY DISPENSING MICROVOLUMES OF FLUIDS

System and method for automatically setting operating parameters for microdispensing devices

Apparatus and method for fabricating high density microarrays and applications thereof

High sensitivity protein workstation and techniques

HIGH SENSITIVITY PROTEIN WORKSTATION AND TECHNIQUES

Molecular diagnostic assay system

Improved sub-assemblies and methods of control for use in a diagnostic assay system adapted to receive an assay cartridge are provided herein. Such sub-assemblies include: a brushless DC motor, a door opening/closing mechanism and cartridge loading mechanism, a syringe and valve drive mechanism assembly, a sonication horn, a thermal control device and optical detection/excitation device. Such systems can further include a communications unit configured to wirelessly communicate with a mobile device of a user so as to receive a user input relating to functionality of the system with respect to an assay cartridge received therein and relaying a diagnostic result relating to the assay cartridge to the mobile device.

Micropump with sonic energy generator

INTEGRATED MULTILAYERED MICROFLUIDIC DEVICES AND METHODS FOR MAKING THE SAME

A multilayered microfluidic device having a substantially monolithic structure is formed by sintering together a plurality of green-sheet layers. The substantially monolithic structure has an inlet port for receiving fluid, an outlet port for releasing fluid, and an interconnection between the inlet port and the outlet port. The substantially monolithic structure may also include a variety of components to enable useful interaction with the fluid, such as electrically conductive pathways, heaters, fluid sensors, fluid motion transducers, and optically transmissive portions. The components are preferably fabricated using thick-film or green-sheet technology and are preferably co-fired with and sintered to the green-sheet layers to become integral with the substantially monolithic structure. By using an adhesive to bind the green-sheet layers together, the multilayered microfluidic device may be fabricated without the application of high pressures. Selection of an adhesive with a polymer ...

METHOD AND APPARATUS FOR SORTING PARTICLES

A sorting method is disclosed in which particles suspended in a fluid are conducted in a closed duct and pass a measurement location in which particles to be selected trigger a signal by a sensing device. At a downstream fork, a pressure wave generated in response to the signal diverts the stream containing the particles from one branch to the other. An apparatus for carrying out this method is disclosed and includes a supply duct for the particle stream, a measurement duct, a measurement position in the measurement duct, a fork downstream of the measurement duct leading to a sorting branch duct and waste branch duct, and a pressure wave generator which is disposed in one of the ducts leading away from the fork.

METHOD AND APPARATUS FOR DISPENSING A LIQUID WITH A PIPETTING NEEDLE

A method and a micropipetting apparatus for dispensing a liquid volume into a vessel by means of a pipetting needle and without any contact between said needle and a liquid contained in said vessel. The method comprises forming a drop (17) at the delivery tip (22) of the pipetting needle (11), said drop being retained at the tip by adhesion forces, and ejecting said drop (17) from said tip (22) by focusing at the tip of the pipetting needle (11) a mechanical excitation wave applied to an excitation point at some distance from said needle. The apparatus comprises a pipetting needle (11), an electromechanical transducer (32) mechanically connected with said pipetting needle (11), electrical signal generating means (33) for generating an excitation pulse (26) signal and for applying this signal to said electromechanical transducer (32) for mechanically exciting said pipetting needle (11) with a pulse of mechanical waves that propagate through said needle.

MICROFABRICATED SEPARATION DEVICE EMPLOYING A VIRTUAL WALL FOR INTERFACING FLUIDS

A fluid interface port (15) in a separation device for separating a sample into different components is provided. The separation device includes an array of separation channels (500) and the fluid interface port (17) comprises an opening formed in the side wall of a separation channel (500) sized and dimensioned to form a virtual wall (15) when the separation channel is filled with a separation medium (20). The fluid interface port (17) is utilized to introduce a liquid sample (19a) into the separation medium (20). The interface ports (17) formed in the array of separation channels (500) are organized into one or more sample injectors. A cathode reservoir is multiplexed with one or more separation channels. To complete an electrical path, an anode reservoir which is common to some or all separation channels (500) is also provided.

TARGET PLATE FOR MASS SPECTOMETERS AND USE THEREOF

A plate suitable for use with mass spectrometers comprising a number of target spots arranged in a surface portion of said plate making it possible to deposit small amounts of fluid at said target spots without the fluid escaping or getting mixed with fluid deposited at another target surface of the same plate. The target surfaces being arranged in a surface portion of said plate so that a base material of said plate constitutes the walls of receptacles, characterised in that the shape, size, temperature and possible agents of said receptacle facilitate evaporation of a solution in which sample molecules are suspended. Embodiments include different types of matrix and enzymes arranged at said spots, and methods for enhancing MALDI analysis efficiency.

HIGH SENSITIVITY PROTEIN WORKSTATION AND TECHNIQUES

A method of selecting and identifying bio-molecules present in a bio-sample is disclosed. The method comprises the steps of: obtaining a bio-sample; amplifying (2) the bio-molecules present in the bio-sample to improve the ease of detection of said bio-molecules; separating the bio-molecules in said amplified bio-sample; depositing (3) the amplified bio-molecules on to a suitable media. Detecting means are then used to identify or detect the presence of bio-molecules in said amplified and separated sample wherein said amplification step occurs in close physical proximity to said deposition step. A device (1) for carrying out the method is also disclosed as is a protein chip library produced by the device or method.

MICROCHIP-BASED ACOUSTIC TRAPPING OR CAPTURE OF CELLS FOR FORENSIC ANALYSIS AND RELATED METHOD THEREOF

Device to the delivery and/or admission/delivery of liquid samples.

DEVICE OF EXEMPTION OF DROPLETS MICROFLUIDIQUES IN PARTICULAR FOR LACYTOMETRIE.

Dispenser or applicator forming localized deposits of biological solutions, comprises flat silicon lever including a central body and pointed tip with slit or groove

L'invention concerne un dispositif de dépôt de solutions biologiques, caractérisé en ce qu'il comporte au moins un levier plan (1) en silicium présentant un corps central et une région d'extrémité (2) formant une pointe (3) dans laquelle est ménagée une fente ou une rainure (5). L'invention concerne également un procédé de fabrication de ce dispositif, caractérisé en ce qu'il met en oeuvre: : a) au moins un dépôt d'oxyde de silicium sur une face avant d'un substrat silicium sur isolant présentant une couche isolante enterrée, b) au moins une attaque chimique ou gravure ionique par la face avant du substrat silicium pour définir le contour des leviers, et au moins une fente ou rainure, le contour des leviers étant défini par attaque chimique ou gravure ionique jusqu'à la couche isolante enterrée, c) une attaque chimique ou gravure ionique par la face arrière du substrat pour enlever y compris la couche isolante enterrée et libérer au moins un levier.

계측 장치 및 이에 사용되는 마이크로튜브의 제조 방법

... 본 발명은 광학 측정 장비를 다른 것으로 대체하여 크기를 줄일 수 있는 계측 장치를 제공하는 것이 그 기술적 과제이다. 이를 위해, 본 발명의 계측 장치는, 미세유체나 이에 부유하는 입자를 계측하기 위한 계측 장치로, 상기 미세유체를 수용하는 마이크로튜브; 상기 마이크로튜브를 가진시키는 액추에이터; 및 상기 마이크로튜브가 가진되면 이를 전류 신호로 변환시키는 튜닝 포크를 포함한다.

CHIP FOR SEPARATING BLOOD AND DIAGNOSIS DEVICE EQUIPPED WITH THE SAME

apparatuses and methods for acoustically manipulate one or more particles

APPARATUS WITH ACTUATING SENSOR MODULE

Device and method useable for integrated sequential separation and enrichment of proteins

DEVICE AND METHOD FOR MICROFLUIDIC TRANSPORT

SYSTEM FOR ASSESSING THE EFFICACY OF STORED RED BLOOD CELLS USING MICROVASCULAR NETWORKS

A system for assessing the microvascular fitness of a sample of stored red blood cells. The system has a network device having at least one network unit. The network unit has a single inlet and a single outlet for the sample and a plurality of microchannels. The plurality of microchannels receive the sample from the single inlet and drain the sample into the single outlet. The network unit includes an aspiration pressure means for providing movement of liquid sample through the at least one network unit. The system further includes an analysis unit that receives the network device therein. The analysis unit includes a sensor for capturing measurements related to the sample and a processor capable of comparing the captured measurements to measurements stored in a database of healthy red blood cells to determine the microvascular fitness of the stored red blood cells.

DNA ANALYSIS MICRO-CHANNEL CHIP

A DNA analysis micro-channel chip which is for use in PCR analysis of DNA contained in a sample, and in which silicon (first layer) and plastic (second layer) are layered. The second layer is layered on a region on part of the first layer, and is provided with reagents, a fluid delivery mechanism and a sensor. The first layer is provided with a PCR reactor on a region on part of the first layer on which the second layer is not layered.

DISPENSER ARRAY SPOTTING

A method of dispensing a fluid comprising (a) aspirating a first fluid volume into a dispenser adapted to dispense fluid volumes of one microliter or less; (b) dispensing a desired volume of the fluid volume to form a dispensed portion; (c) calculating the volume of the dispensed portion; and (d) calculating an adjusted desired volume that compensates for a difference between the desired volume and the volume of the dispensed portion.

METHOD AND APPARATUS FOR THE DISCRETIZATION AND MANIPULATION OF SAMPLE VOLUMES

Embodiments of the present invention relate to methods and apparatuses for the discretization and manipulation of sample volumes that is simple, robust, and versatile. It is a fluidic device that partitions a sample by exploiting the interplay between fluidic forces, interfacial tension, channel geometry, and the final stability of the formed droplet and/or discretized volume. These compartmentalized volumes allow for isolation of samples and partitioning into a localized array that can subsequently be manipulated and analyzed. The isolation of the discretized volumes along with the device's inherent portability render our invention versatile for use in many areas, including but not limited to PCR, digital PCR, biological assays for diagnostics and prognostics, cancer diagnosis and prognosis, high throughput screening, single molecule and single cell reactions or assays, the study crystallization and other statistical processes, protein crystallization, drug screening, environmental testing ...

METHOD AND SYSTEM FOR PRECISE DISPENSATION OF A LIQUID

Devices, systems, and methods for the precise dispensation of small volumes of liquids are presented. A fluid microdispenser comprises a liquid-filled tube, an actuator coupled to the tube, and a tip at one end of the tube. When the actuator applies an actuation pressure, a precise volume of liquid is ejected from the orifice of the tip. The orifice is manufactured to control the volume of liquid ejected by each actuation and, therefore, may have a diameter smaller than that of the liquid-filled tube. The invention comprises systems, methods, and devices so that the inner diameter of the liquid-filled tube is decreased (tapered) to that of the orifice so as to maximize transmission to the orifice of the pressure generated by the actuation stimulus. This enables the volume of liquid ejected by each stimulus to be reproducibly controlled by the amplitude of the actuation stimulus.

METHOD FOR ANALYSING MACROMOLECULES, ANALYSIS DEVICE AND A METHOD FOR PRODUCING AN ANALYSIS DEVICE

The invention relates to a method for analysing macromolecules using a microarray, which consists of several spots in a known arrangement. Said spots comprise a plurality of first macromolecules that differ at least partially. The microarray is located on the surface of a solid body, preferably a chip. According to the method, at least one drop of liquid containing macromolecules is applied to the surface of the solid body, the quantity of liquid being so small that only one spot or a small group of spots of the microarray comes or come into contact with the drop of liquid. After a predetermined time has elapsed, the drop of liquid is displaced onto a subsequent spot or an additional small group of spots with the help of the transmission pulse of a surface sound wave. The spots that come into contact with the drop of liquid are then analysed to identify whether a reaction with the material of said drop has occurred. The invention also relates to a method for producing an analysis device ...

MICROREACTOR

A microreactor has flow paths consisting of microchannels, a reactor connected to the flow paths, inlet openings for sending a sample to and receiving a sample from the flow paths or the reactor that is connected to the flow paths, a first base plate having outlet openings for recovering the sample after reaction, and a second base plate where at least one or more micropumps are formed so as to correspond to the inlet openings and/or outlet openings. Each micropump has a base body having in its inner surface a cavity made from ceramic, an electrode and piezoelectric/electrostriction layer formed on the outer surface of the base body, and at least two or more connection openings. The first and the second base plate are adhered to each other and integrally formed.

METHOD OF DENATURING AND FRAGMENTING DNA OR RNA USING ULTRASOUND

A method and apparatus are provided for processing a DNA or RNA sample within a sample processing module. The method includes the steps of providing a sample well within the sample processing module that contains the DNA or RNA sample, coupling ultrasonic energy from an external source into the sampling well and denaturing and fragmenting the DNA or RNA sample using the ultrasonic energy.

SYSTEM, METHOD AND COMPUTER IMPLEMENTED PROCESS FOR ASSAYING COAGULATION IN FLUID SAMPLES

A sample analyzing system includes at least one sensor located at least partially within a sample retaining area. In addition, the sensor has at least one edge defining a sample detection location. This sample detection location defines an area within which the sensor is capable of detecting a presence or an absence of the sample. The system analyzes sample data by first introducing the sample into the sample retaining area and then mixing a reagent with the sample to commence formation of a reagent product. After mixing and upon detecting the absence of the sample from the sample detection location by the at least one sensor, an edge of the sample is moved past an edge of the at least one sensor and into the sample detection location. Then, upon detecting the presence of the sample in the sample detection location by the at least one sensor, the edge of the sample is moved past the edge of the at least one sensor and out of the sample detection location. Additionally, between oscillations ...

ELECTRONIC CONTROL OF FLUIDIC SPECIES

Various aspects of the present invention relate to the control and manipulation of fluidic species, for example, in microfluidic systems. In one aspect, the invention relates to systems and methods for making droplets of fluid surrounded by a liquid, using, for example, electric fields, mechanical alterations, the addition of an intervening fluid, etc. In some cases, the droplets may each have a substantially uniform number of entities therein. For example, 95% or more of the droplets may each contain the same number of entities of a particular species. In another aspect, the invention relates to systems and methods for dividing a fluidic droplet into two droplets, for example, through charge and/or dipole interactions with an electric field. The invention also relates to systems and methods for fusing droplets according to another aspect of the invention, for example, through charge and/or dipole interactions. In some cases, the fusion of the droplets may initiate or determine a reaction ...

MICRODOSING DEVICE AND METHOD FOR OPERATING SAME

The invention relates to a microdosing device comprising a pressure chamber (24) which is at least partly delimited by a displacer (10), an actuating device (30) for actuating the displacer (10), whereby actuation of said displacer (10) is able to alter the volume (28) of the pressure chamber (24), a storage reservoir for a medium, which is connected to the pressure chamber (24) via a first fluid line (20) in such a way that fluid is able to circulate, and an outlet opening (26) which is connected to the pressure chamber (24) via a second fluid line (22) also in such a way that fluid is able to circulate. The microdosing device further comprises a device (12, 14) for determining the position of the displacer (10) at any given time and a control device which is connected to the actuating device (30) and the device (12, 14) for detecting the position of the displacer (10). The control device controls the actuating device (30) on the basis of the detected displacer (10) position or on the ...

PROGRAMMABLE ULTRASONIC FIELD DRIVEN MICROFLUIDICS

In one aspect a high frequency ultrasonic microfluidic flow control device is disclosed. The device includes an array of ultrasonic transducers arranged to direct ultrasound to a microfluidic channel. The device further includes one or more driver circuits. Each ultrasonic transducer is associated with one of the one or more driver circuits, and each ultrasonic transducer is driven by a driver signal from the associated driver circuit. The array of ultrasonic transducers and one or more driver circuits are produced in the same semiconductor fabrication process. The device further includes one or more electrical contacts associated with each ultrasonic transducer in the array if ultrasonic transducers, wherein the one or more electrical contacts associated with each ultrasonic transducer applies the driver signal from the associated ultrasonic driver circuit.

Fabricating arrays with drop velocity control

A method for fabricating a chemical array with multiple features. The method may include ejecting drops from an ejection head spaced from a substrate surface and during movement relative to the substrate surface, onto the substrate surface while varying an ejection velocity of the drops according to a predetermined pattern. An apparatus and computer program product which can execute such the foregoing method are also provided.

Micromechanical ejection pump for separating small fluid volumes from a flowing sample fluid

A micromechanical ejection pump for extracting small fluid volumes from a flowing sample fluid is provided with a substrate, and a canal for pressurized working fluid. The micromechanical ejection pump also has a canal for ejecting a fluid volume from the sample fluid. The working fluid canal runs into the extension of the sample fluid canal discharging crosswise thereto. The ejection canal connected to the sample fluid canal branches off from the working fluid canal opposite to the sample fluid canal. The working fluid canal and the ejection canal have in particular a substantially similar cross-section of 0.01-0.12 mm2, wherein the ratio of the cross-section of the sample fluid canal to the cross-section of the working fluid canal or the ejection canal is preferably between 0.2 and 0.5, more particularly between 0.3 and 0.4 and most preferably 0.35.

Use of microfluidic systems in the electrochemical detection of target analytes

The invention relates generally to methods and apparatus for conducting analyses, particularly microfluidic devices for the detection of target analytes.

Rapid thermal cycling device

A lid for a well plate is provided. Pins penetrate the lid and project downwardly into the wells of the well plate. Thermal energy is applied to the upper end of the pin to regulate the temperature of liquid samples in the well plate. Ultrasonic energy is applied to the upper end of the pin to sonicate the liquid sample. An electrical charge is applied to the upper end of the pin to attract charged materials, such as DNA, and to selectably segregate charged materials from the liquid sample.

Particle sorting apparatus, particle sorting method, and non-transitory computer-readable storage medium storing program

The present disclosure provides a particle sorting apparatus, a particle sorting method, and a non-transitory computer-readable storage medium storing program that enable sorting object particles to be sorted with high precision, even when the sorting object particles are large. In the particle sorting apparatus, a charging unit that applies charges to at least a part of liquid droplets ejected from an orifice to generate a fluid stream and a charging control unit that adjusts a charge application end time in the charging unit according to sizes of particles included in the liquid droplets are provided.

Microchemical chip

A microchemical chip (10A) comprises a plate-shaped substrate (12), with a channel (14) formed on a surface of the substrate (12) through which a fluid flows. A fluid storage section (16) for storing the fluid communicates with the channel (14) at a starting end of the channel (14). A fluid discharge section (18) communicates with the channel (14) at a terminal end of the channel (14). An extruding pump section (22) is formed integrally on the substrate (12), at a portion of the channel (14) in the vicinity of the fluid storage section (16).

MICROFLUIDIC DEVICE

A microfluidic device may include a first fluid chamber, a second fluid chamber, a first microfluidic passage extending between the first fluid chamber and the second fluid chamber, a second microfluidic passage extending from the second fluid chamber, a first fluid actuator adjacent the first microfluidic passage and proximate the first fluid chamber to inertially pump fluid away from the first fluid chamber and a second fluid actuator adjacent the first microfluidic passage and proximate the second fluid chamber to menially pump fluid towards the first fluid chamber.

COMPONENT SEPARATING DEVICE AND CHEMICAL ANALYSIS DEVICE USING THE SAME

A vibrator has a large strength of a standing wave even with a low driving voltage, thereby improving the accuracy of component separation. A device according to the present invention includes a substrate having a channel groove provided in an upper surface of the substrate, a seal provided above the substrate so as to cover an upper opening of the channel groove, a projection provided on an outer side wall opposite to the channel groove, and a vibrator causing the projection to warp and vibrate in a depth direction of the channel groove. The warping vibration of the projection is amplified due to effect of leverage, and generates a large stress on the outer wall of the channel groove having the projection provided thereon. Consequently, the strength of a standing wave in the channel groove increases even for a low driving voltage, thereby improving the accuracy of component separation.

PARTICLE SEPARATION

An example system includes an input channel having a first end and a second end to receive particles through the first end, a separation chamber, at least two output channels, and an integrated pump to facilitate flow through the separation chamber. The separation chamber is in fluid communication with the second end of the input channel. The separation chamber has a passive separation structure, the passive separation structure including an array of columns spaced apart to facilitate separation of particles in a flow based on a size of the particles. Each output channel is in fluid communication with the separation chamber to receive separated particles. The integrated pump is positioned within at least one of the input channel or one of the at least two output channels.

Inkjet Device and Method for the Controlled Positioning of Droplets of a Substance Onto a Substrate

The invention provides a device for the controlled positioning of droplets of a substance onto a substrate, the device comprising at least a print head comprising a nozzle provided to eject the droplet, the inkjet device further comprising a control camera arranged such that after ejection of the droplet out of the nozzle, the droplet is detected by the control camera. In case either the droplet volume or velocity or flight path or viscosity or surface tension start to deviate from preset values, the software can correct for this in a closed loop manner. If the droplet is not ejected at all, the control software stops the printer and an operator can maintain the print head.

Plate washing system with ultrasonic cleaning of pipes

A plate washing system and method of cleaning pipes of the plate washing system. The plate washing system includes at least one manifold having a plurality of pipes configured to be provided within wells of a plate in order to wash the wells, at least one manifold having a plurality of pipes, a tank, an ultrasonic transducer mounted to the tank, and a control system. When tips of the pipes are positioned within the tank, the control system activates the ultrasonic transducer in order to vibrate a fluid within the tank.

Method and device for biomolecule preparation and detection using magnetic array

An embodiment of the invention relates to a device for detecting an analyte in a sample. The device comprises a fluidic network and an integrated circuitry component. The fluidic network comprises multiple zones such as a sample zone, a cleaning zone and a detection zone. The fluidic network contains a magnetic particle and/or a signal particle. A sample containing an analyte is introduced, and the analyte interacts with the magnetic particle and/or the signal particle through affinity agents. A microcoil array or a mechanically movable permanent magnet is functionally coupled to the fluidic network, which are activatable to generate a magnetic field within a portion of the fluidic network, and move the magnetic particle from the sample zone to the detection zone. A detection element is present which detects optical or electrical signals from the signal particle, thus indicating the presence of the analyte.

Microfluidic device, driving method thereof, and microfluidic system

The present disclosure provides a microfluidic device, a driving method thereof and a microfluidic system. The microfluidic device includes a first substrate and a second substrate disposed opposite to each other, and a microcavity provided between the first and second substrates for accommodating droplets. The microfluidic device further includes at least one ultrasonic layer provided between the first and second substrates. The at least one ultrasonic layer includes a plurality of ultrasonic sensors configured to perform at least one of detection operation and driving operation to the droplets accommodated in the microcavity.

METHOD AND DEVICE FOR MANIPULATING SMALL QUANTITIES OF LIQUID

Method for mixing droplets in a microchannel

Various aspects of the present invention relate to the control and manipulation of fluidic species, for example, in microfluidic systems. In one aspect, the invention relates to systems and methods for making droplets of fluid surrounded by a liquid, using, for example, electric fields, mechanical alterations, the addition of an intervening fluid etc. In some cases, the droplets may each have a substantially uniform number of entities therein. For example, 95% or more of the droplets may each contain the same number of entities of a particular species. In another aspect, the invention relates to systems and methods for dividing a fluidic droplet into two droplets, for example, through charge and/or dipole interactions with an electric field. The invention also relates to systems and methods for fusing droplets according to another aspect of the invention, for example, through charge and/or dipole interactions. In some cases, the fusion of the droplets may initiate or determine a reaction ...

METHOD FOR FILLING SAMPLE CARRIERS

The invention relates to a method for filling sample carriers (26) with a chemical and/or biological liquid. Said method can be carried out by means of a liquid releasing device (10) comprising a liquid chamber (12), a piezoelectric actuator (22) acting on said liquid chamber (12). The production of an exciting pulse by means of the piezoelectric actuator (22) causes liquid drops (24) to be expelled from an outlet (16) towards a sample carrier (26), thus filling recesses (28). In order to avoid wetting (30) in the region of the outlet (16), a pre-pulse (40) is produced before the exciting pulses (34) are produced, and a damping pulse (46) is produced once the filling process is completed.

LIQUID PORTIONING METHOD AND DEVICE

Methods and apparatuses for dispensing samples or reagents onto a target object are provided. A dispensing position designating section (62) designates the dispensing position on the target object (50), such as a membrane, based on an image of the target object (50) displayed on a monitoring section (60). A dispensing control section (64) positions the target object and the dispensing device relative to each other so that the designated dispensing position on the target object is placed beneath the dispensing device (10) and controls the dispensing operation of the dispensing device (10). A dispensing position information creating section (68) creates dispensing position information about the dispensing position on the target object (50) which is designated by the dispensing position designating section (62) and to which the dispensing operation has been performed and outputs it to the outside.

A DEVICE FOR DISPENSING DROPS OF A LIQUID

Micro total analysis chip and micro total analysis system

MICRODOSING DEVICE AND METHOD FOR ITS OPERATION

MICROFLUIDIC SYSTEMS AND METHODS FOR SORTING PARTICLES

DURCHFLUSSZELLE FÜR PROBEN UND DEREN VERWENDUNG

Sortiervorrichtung für biologische Zellen oder Viren

MIKROMECHANISCHE EJEKTIONSPUMPE ZUM HERAUSTRENNEN KLEINSTER FLUIDVOLUMINA AUS EINEM STRÖMENDEN PROBENFLUID

NO DETAILS

USE FROM MICRO-FLUID SYSTEMS TO ELECTRO-CHEMICAL DETECTION OF ZIELANALYTEN

PROCEDURE AND DEVICE FOR THE PRODUCTION OF MOVEMENT IN A THIN LIQUID FILM

DEVICE FOR THE EXECUTION OF CHEMICAL REGULATIONS

PROCEDURE FOR THE ACTIVATION OF CHEMICAL OR CHEMICO-PHYSICAL PROCESSES BY SIMULTANEOUS USE OF MICROWAVES AND ULTRASONIC PULSES AND CHEMICAL REACTOR FOR IT

PROCEDURE AND DEVICE FOR THE CONTACTLESS DROPLET DELIVERY UNDERNEATH A FLUID SURFACE

PROCEDURE AND DEVICE FOR THE MANIPULATION OF SMALL LIQUID QUANTITIES

PROCEDURE FOR THE ANALYSIS AND DEVICE

MICROMECHANICAL EJEKTIONSPUMPE FOR RIPPING SMALLEST ONES FLUID VOLUMES OUT FROM A FLOWING SAMPLE FLUID

SYSTEM, PROCEDURE AND COMPUTER-IMPLEMENTED PROCESS FOR THE EXAMINATION OF COAGULATION IN LIQUID SAMPLES

SYSTEM, PROCEDURE AND COMPUTER-IMPLEMENTED PROCESS FOR THE EXAMINATION OF COAGULATION IN LIQUID SAMPLES

SYSTEM, PROCEDURE AND COMPUTER-IMPLEMENTED PROCESS FOR THE EXAMINATION OF COAGULATION IN LIQUID SAMPLES

Test device, reaction apparatus and reactive test method

A test device having a micro flow channel including a reaction part where a reactant that is reactive to a tested chemical dispersed in a tested fluid is fixed, and at least one actuator for actuating the tested fluid to move in at least one of two opposite sides of the micro flow channel so as to homogenize a density distribution of the tested chemical in the tested fluid. The tested fluid is sent in the micro flow channel a plurality of times.

Methods and Microfluidic Devices for the Manipulation of Droplets in High Fidelity Polynucleotide Assembly

Methods and devices are provided for manipulating droplets on a support using surface tension properties, moving the droplets along a predetermined path and merging two droplets together enabling a number of chemical reactions. Disclosed are methods for controlling the droplets volumes. Disclosed are methods and devices for synthesizing at least one oligonucleotide having a predefined sequence. Disclosed are methods and devices for synthesizing and/or assembling at least one polynucleotide product having a predefined sequence from a plurality of different oligonucleotides having a predefined sequence. In exemplary embodiments, the methods involve synthesis and/or amplification of different oligonucleotides immobilized on a solid support, release of synthesized/amplified oligonucleotides in solution to form droplets, recognition and removal of error-containing oligonucleotides, moving or combining two droplets to allow hybridization and/or ligation between two different oligonucleotides, and further chain extension reaction following hybridization and/or ligation to hierarchically generate desired length of polynucleotide products.

Particle Analysis in an Acoustic Cytometer

The present invention is a method and apparatus for acoustically manipulating one or more particles.

Piezoelectric dispenser with a longitudinal transducer and replaceable capillary tube

Systems, devices and methods are provided, for acquiring and dispensing predetermined volumes of liquids and, in particular, to a unique piezoelectric dispenser for acquiring and dispensing of small volumes of fluid in an automatic or manual production of DNA arrays and assays, wherein droplets are dispensed in a single drop format with volumes that may range from about a few picoliters to several nanoliters. The dispenser can advantageously utilize a disposable capillary tube assembly while desirably retaining the piezoelectric actuator for subsequent further uses, thereby mitigating the possibility of cross contamination of fluids and providing an economical and cost effective approach with reuse of the piezoelectric actuator for further operation such as with a variety of liquids to be dispensed and transferred. A unique longitudinal transducer can transmit radial tube displacement into controlled axial motion of the tube.

Mechanically Integrated and Closely Coupled Print Head and Mist Source

A deposition apparatus comprising one or more atomizers structurally integrated with a deposition head. The entire head may be replaceable, and prefilled with material. The deposition head may comprise multiple nozzles. Also an apparatus for three dimensional materials deposition comprising a tiltable deposition head attached to a non-tiltable atomizer. Also methods and apparatuses for depositing different materials either simultaneously or sequentially.

The Use of Microfluidic Systems in the Electrochemical Detection of Target Analytes

The invention relates generally to methods and apparatus for conducting analyses, particularly microfluidic devices for the detection of target analytes.

MAGNETIC SEPARATION DEVICE AND METHOD OF USE

The current invention relates to the method and apparatus to magnetically separate biological entities with magnetic labels from a fluid sample. The claimed magnetic separation device removes biological entities with magnetic labels from its fluidic solution by using a soft-magnetic center pole with two soft-magnetic side poles. The claimed device further includes processes to dissociate entities conglomerate after magnetic separation. 1. A device for separating biological entities comprising:a magnetic field source having a first surface and a second surface;a soft magnetic center pole having a first end contacting said first surface, and a tip end tapering away from said first end, thereby concentrating magnetic flux from said first surface towards said tip end;a first soft magnetic side pole having a first base end and a second end;a second soft magnetic side pole having a second base end and a third end;a channel contacting said tip end contains a fluidic sample comprising said biological entities, thereby causing separation of said biological entities bound with magnetic labels towards said tip end;wherein said first and second base ends contact said second surface;wherein said second and third ends are located on opposing sides of said tip end;wherein said magnetic flux from said tip end divides into each of said second and third ends, thereby forming a flux closure within said center pole and said first and second side poles; andwherein said biological entities bound with magnetic labels are removed from said channel after separating said channel from said tip end.2. The device according to claim 1 , wherein said magnetic field source comprises a permanent magnet having a first polarization surface contacting a top surface of a soft magnetic shield claim 1 , and a second polarization surface opposing said first polarization surface claim 1 , wherein said first surface is said second polarization surface and said second surface is said top surface.3. The ...

ENHANCED CELL/BEAD ENCAPSULATION METHODS AND APPARATUSES

A method of encapsulating a solid sample in a droplet, the method including flowing a continuous phase through a first fluid channel at a first flow rate; flowing a dispersed phase through a second fluid channel at a second flow rate, the dispersed phase including a plurality of particles, cells or beads; trapping the plurality of particles, cells or beads in a mixing region that receives the dispersed phase and the continuous phase; and reducing the first flow rate to encapsulate the trapped particles, cells or beads in droplets of the dispersed phase generated when the dispersed phase and the continuous phase exit the mixing region through an orifice. 1. A method of encapsulating a solid sample in a droplet , the method comprising:flowing a continuous phase through a first fluid channel at a first flow rate;flowing a dispersed phase through a second fluid channel at a second flow rate, the dispersed phase comprising a plurality of particles, cells or beads;trapping the plurality of particles, cells or beads in a mixing region that receives the dispersed phase and the continuous phase; andreducing the first flow rate to encapsulate the trapped plurality of particles, cells or beads in droplets of the dispersed phase generated when the dispersed phase and the continuous phase exit the mixing region through an orifice.2. The method according to claim 1 , wherein a pump and/or a pressure regulator is used to:adjust a flow rate and/or a pressure of the continuous phase or the dispersed phase to trap the plurality of particles cells or beads of the dispersed phase in the mixing region in a first mode such that the plurality of particles, cells or beads of the dispersed phase are prevented from flowing through the orifice; andadjust a flow rate and/or a pressure of the continuous phase or the dispersed phase to allow the plurality of particles, cells or beads to flow through the orifice such that the plurality of particles, cells or beads are encapsulated in droplets of ...

METAL ORGANIC FRAMEWORKS AND METHODS OF PREPARATION THEREOF

A method of preparing a Metal Organic Framework (MOF) with an acoustically-driven microfluidic platform, the method comprising: depositing a liquid comprising MOF precursors on a piezoelectric substrate of an acoustic microfluidic platform, the MOF precursors comprising a metal ion and an organic ligand, applying acoustic irradiation to the liquid to induce azimuthal liquid recirculation, which causes formation of the MOF within the liquid, and isolating the MOF. 1. A method of preparing a Metal Organic Framework (MOF) with an acoustically-driven microfluidic platform , the method comprising:depositing a liquid comprising MOF precursors on a piezoelectric substrate of an acoustic microfluidic platform, the MOF precursors comprising a metal ion and an organic ligand,applying acoustic irradiation to the liquid to induce azimuthal liquid recirculation, which causes formation of the MOF within the liquid, andisolating the MOF.2. A method according to wherein the MOF is at least a partially activated MOF.3. A method according to wherein the MOF is an activated MOF.4. A method according to wherein the MOF has a high degree of orientation.5. A method according to wherein the acoustic irradiation comprises surface acoustic waves claim 1 , bulk acoustic waves or hybrid acoustic waves comprising both surface and bulk acoustic waves.6. A method according to wherein the surface acoustic waves are Rayleigh surface acoustic waves or shear-horizontal surface acoustic waves.7. A method according to wherein the acoustic irradiation comprises travelling or standing acoustic waves.8. A method according to wherein the azimuthal liquid recirculation is induced by off-centre acoustic waves.9. A method according to wherein the acoustic platform comprises at least one interdigitated transducer (IDT) positioned off-centred relative to the liquid comprising MOF precursors to generate off-centre acoustic waves.10. A method according to wherein the acoustic platform comprises two opposing off- ...

GENE TRANSFECTION SYSTEM AND METHOD

The gene transfection system includes an acoustothermal module and a signal generating module; the acoustothermal module includes a piezoelectric substrate, an acoustothermal chip arranged on the piezoelectric substrate and N sound-absorbing vessels arranged on the acoustothermal chip and used for cultivating recipient cells, and N is an integer greater than or equal to 1; the signal generating module is used to output basic frequency signals; the acoustothermal chip is used to convert the basic frequency signal to an acoustic wave signal, establish a temperature gradient field with the acoustic wave signal, and control the temperature of the recipient cell in the sound-absorbing vessels with the temperature gradient field, so that the pores are opened on the membranes of the recipient cell, and the nucleic acids or other substances can go into the cells through these pores. 1. A gene transfection system , wherein the system comprises: an acoustothermal module and a signal generating module;the acoustothermal module comprises: a piezoelectric substrate, an acoustothermal chip arranged on the piezoelectric substrate, and N sound-absorbing vessels arranged on the acoustothermal chip and configured for culturing recipient cells, wherein N is an integer greater than or equal to 1;the signal generating module is configured to output a basic frequency signal;the acoustothermal chip is configured to convert the basic frequency signal to an acoustic wave signal, establish a temperature gradient field in the sound-absorbing vessels with the acoustic wave signal, and precisely control the temperature of the recipient cells in the sound-absorbing vessels with the temperature gradient field, so that the pores are opened on the membranes of the recipient cells, and the nucleic acids or other substances can go into the cells through these pores.2. The gene transfection system of claim 1 , wherein the acoustothermal chip comprises M interdigital transducers claim 1 , a first drive ...

Microfluidic transport

A device for microfluidic transport includes a first fluid reservoir, a second fluid reservoir spaced from the first fluid reservoir, a main fluid channel communicated with the first fluid reservoir and the second fluid reservoir, an auxiliary fluid channel communicated with the main fluid channel, and a fluid actuator within the auxiliary fluid channel asymmetric to the main fluid channel such that operation of the fluidic actuator is to induce fluid flow in the main fluid channel from the first reservoir toward the second reservoir.

PARTICLE SORTING APPARATUS, PARTICLE SORTING METHOD, AND NON-TRANSITORY COMPUTER-READABLE STORAGE MEDIUM STORING PROGRAM

The present disclosure provides a particle sorting apparatus, a particle sorting method, and a non-transitory computer-readable storage medium storing program that enable sorting object particles to be sorted with high precision, even when the sorting object particles are large. In the particle sorting apparatus, a charging unit that applies charges to at least a part of liquid droplets ejected from an orifice to generate a fluid stream and a charging control unit that adjusts a charge application end time in the charging unit according to sizes of particles included in the liquid droplets are provided. 1. A particle sorting apparatus , comprising:a charging unit configured to apply charges to at least a portion of a plurality of liquid droplets ejected from an orifice; and wherein the mode is selected from one of a first mode or a second mode, based on a size of particles included in the plurality of liquid droplets, and', 'wherein at least one of the charge application end time or a charge application duration of the first mode is different from the second mode., 'a charging control unit configured to adjust a charge application end time for the application of the charges, based on a mode of the particle sorting apparatus,'}2. The particle sorting apparatus according to claim 1 , wherein the first mode is a normal mode and the second mode is a large diameter particle mode.3. The particle sorting apparatus according to claim 1 , wherein the charging control unit is further configured to change a start time for the application of the charges based on sizes of the plurality of particles.4. The particle sorting apparatus according to claim 1 , wherein the charging control unit is further configured to change the charge application duration based on sizes of the plurality of particles.5. The particle sorting apparatus according to claim 1 ,wherein the orifice is present in an exchangeable microchip, andwherein the charging unit includes a charging electrode to contact ...

Test device, reaction apparatus and reactive test method

A test device having a micro flow channel including a reaction part where a reactant that is reactive to a tested chemical dispersed in a tested fluid is fixed, and at least one actuator for actuating the tested fluid to move in at least one of two opposite sides of the micro flow channel so as to homogenize a density distribution of the tested chemical in the tested fluid. The tested fluid is sent in the micro flow channel a plurality of times.

SYSTEMS AND METHODS FOR TAGGING AND ACOUSTICALLY CHARACTERIZING CONTAINERS

Embodiments of the present invention provide systems and methods for tagging and acoustically characterizing containers. 1providing the container, the container: originating from within a mold and comprising at least one vertical sidewall; and a bottom coupled to the at least one vertical sidewall wherein the bottom has an interior side, an exterior side, and a thickness and includes a plurality of recesses, grooves, or protrusions on the exterior side for acoustic characterization of the container;transmitting an acoustic signal toward the bottom;receiving a plurality of reflections of the acoustic signal, wherein the plurality of reflections comprises a first reflection from a first subset of the plurality of recesses, grooves, or protrusions a second reflection from a second subset of the plurality of recesses, grooves, or protrusions, the first reflection having a first time of flight and the second reflection having a second time of flight; andcharacterizing the container based on the first and second times of flight, wherein the characterizing comprises determining a characteristic of the container,wherein (a) the characteristic is associated with a location in the mold from which the container originates or (b) the mold is one of a plurality of molds and the characteristic is associated with the mold.. A method for acoustically characterizing a container configured to hold a fluid, the method comprising: This application is a continuation of U.S. patent application Ser. 16,705,807, filed Dec. 6, 2019, which is a continuation of U.S. patent application Ser. 15/289,692, filed Oct. 10, 2016, which claims the benefit of U.S. Provisional Patent Application No. 62/240,412, filed Oct. 12, 2015, and all entitled “Systems and Methods for Tagging and Acoustically Characterizing Containers,” the entire contents of all applications are incorporated by reference herein.This application relates to tagging containers, such as containers formed using injection molding. But ...

Spatial Separation of Particles in a Particle Containing Solution for Biomedical Sensing and Detection

A device and method for analyte detection and analytes in a particulate bearing fluid such as whole blood having an instrument for partitioning the particles from the fluid that is integrated with a detector for analyses of one or more particulate bearing fluid analytes while the particles in the particulate bearing fluid are partitioned. 1) A device for analyzing analytes in a complex particulate fluid , said complex particulate fluid comprising a fluid portion comprising an analyte , and a particulate portion , said device comprising:a microchannel capable of housing a column of the complex particulate fluid, the microchannel comprising at least one analyte detection region;an acoustic transducer capable of generating acoustic waves, said acoustic tranducer aligned with the at least one analyte detection region, said acoustic waves partitioning said particulate portion from said fluid portion in said analyte detection region of said microchannel; and,a detector of said analyte in said fluid portion aligned with the analyte detection region of the microchannel.2) The device of further comprising a fluid flow arrestor for arresting flow of the fluid at the analyte detection region.3) The device of wherein said complex fluid comprises whole blood and said fluid portion comprises plasma.4) The device of wherein said acoustic waves are selected from the group consisting of ultrasonic standing waves claim 1 , surface acoustic waves claim 1 , bulk acoustic waves claim 1 , and acoustic waves with frequency preferably in the range of 2 kHz to 2 GHz.5) The device of further comprising a plurality of acoustic transducers claim 1 , a plurality of analyte detection regions claim 1 , and a plurality of analyte detectors.6) The device of further comprising a fluid collector for collecting fluid after analysis of the analyte.7) The device of wherein said complex fluid collector comprises a microchannel claim 6 , a pocket claim 6 , a dilatation claim 6 , a chamber claim 6 , or a ...

Microfluidic Chip for Acoustic Separation of Biological Objects

A microfluidic device for sorting biological objects includes a microfluidic chip including a planar substrate having first and second planar surfaces, the planar substrate including first and second networks of channels recessed respectively from the first and second planar surfaces and fluidically connected by way of at least a through-hole in the planar substrate; a first lid attached to the first planar surface of the planar substrate and substantially covering the first network of channels; and a second lid attached to the second planar surface of the planar substrate and substantially covering the second network of channels; and one or more piezoelectric transducers attached to the first lid and/or the second lid and configured to generate first and second acoustic standing waves in a first linear channel of the first network of channels and a second linear channel of the second network of channels, respectively. 1. A microfluidic device for sorting biological objects comprising:{'claim-text': ['a planar substrate having first and second planar surfaces, the planar substrate including first and second networks of channels recessed respectively from the first and second planar surfaces and fluidically connected in series or parallel by way of at least a through-hole in the planar substrate;', 'a first lid attached to the first planar surface of the planar substrate and substantially covering the first network of channels; and', 'a second lid attached to the second planar surface of the planar substrate and substantially covering the second network of channels; and'], '#text': 'a microfluidic chip including:'}one or more first piezoelectric transducers attached to an exterior of the first lid and configured to generate a first acoustic standing wave having a first wavelength in a first linear channel of the first network of channels.2. The microfluidic device of further comprising one or more second piezoelectric transducers attached to an exterior of the second ...

Device having actuating and sensing module

A device includes a main body and an actuating and sensing module. A length of the main body is 0.2˜6 mm. A width of the main body is 0.1˜5.5 mm. A height of the main body is 0.1˜2.5 mm. The actuating and sensing module is disposed in the main body. The actuating and sensing module includes a carrier, a sensor, an actuator, a driving-and-transporting controller and a battery. The sensor, the actuator, the driving-and-transporting controller and the battery are disposed on the carrier. The actuator is disposed on one lateral side of the sensor. The actuator has a fluid channel. The actuator is enabled to transport fluid, so that the fluid flows through the fluid channel toward the sensor, and the sensor measures the fluid received therethrough.

Devices having a plurality of droplet formation regions

Devices, systems, and their methods of use, for generating droplets are provided. One or more geometric parameters of a microfluidic channel can be selected to generate droplets of a desired and predictable droplet size.

Method and device for biomolecule preparation and detection using magnetic array

An embodiment of the invention relates to a device for detecting an analyte in a sample. The device comprises a fluidic network and an integrated circuitry component. The fluidic network comprises multiple zones such as a sample zone, a cleaning zone and a detection zone. The fluidic network contains a magnetic particle and/or a signal particle. A sample containing an analyte is introduced, and the analyte interacts with the magnetic particle and/or the signal particle through affinity agents. A microcoil array or a mechanically movable permanent magnet is functionally coupled to the fluidic network, which are activatable to generate a magnetic field within a portion of the fluidic network, and move the magnetic particle from the sample zone to the detection zone. A detection element is present which detects optical or electrical signals from the signal particle, thus indicating the presence of the analyte.

Vibration-driven droplet transport devices

Methods and devices are provided for moving a droplet on an elongated track formed on a patterned surface using vibration. The elongated track includes a plurality of patterned transverse arcuate regions such that when the surface is vibrated the droplet is urged along the track as a result of an imbalance in the adhesion of a front portion of the droplet and a back portion of the droplet to the transverse arcuate regions.

Actuating and sensing module

An actuating and sensing module is provided. The actuating and sensing module includes at least one sensor, at least one actuating device and a power storage member. The sensor is disposed for measuring fluid. The actuating device is disposed proximate to the sensor and is disposed for transporting the fluid. The power storage member is configured as a graphene battery and is disposed for providing power to the at least one sensor and the at least one actuating device for driving the at least one sensor and the at least one actuating device. The actuating device is driven to transport the fluid toward the sensor so as to make the fluid measured by the sensor.

MICROFLUIDIC CHANNELS TO CONVEY CELLS OF DIFFERENT SIZES

An example device includes a first microfluidic channel in communication with a fluid reservoir to receive cell-containing fluid from the fluid reservoir. The device further includes a second microfluidic channel in communication with the fluid reservoir to receive cell-containing fluid from the fluid reservoir. The device further includes a first sensor disposed at the first microfluidic channel, a second sensor disposed at the second microfluidic channel, a first dispense nozzle disposed at an end of the first microfluidic channel, and a second dispense nozzle disposed at an end of the second microfluidic channel. The first microfluidic channel is shaped to convey cells of a first size range, and the second microfluidic channel is shaped to convey cells of a second size range that is different from the first size range. 1. A device comprising:a first microfluidic channel, the first microfluidic channel in communication with a fluid reservoir to receive cell-containing fluid from the fluid reservoir;a second microfluidic channel, the second microfluidic channel in communication with the fluid reservoir to receive cell-containing fluid from the fluid reservoir;a first sensor disposed at the first microfluidic channel;a second sensor disposed at the second microfluidic channel;a first dispense nozzle disposed at an end of the first microfluidic channel; anda second dispense nozzle disposed at an end of the second microfluidic channel;wherein the first microfluidic channel is shaped to convey cells of a first size range, and the second microfluidic channel is shaped to convey cells of a second size range that is different from the first size range.2. The device of claim 1 , further comprising a surface feature to extend into the second microfluidic channel to guide cells into sensing proximity of the second sensor.3. The device of claim 1 , further comprising:a substrate to carry the first microfluidic channel, the first sensor, the first dispense nozzle, the second ...

MICROCHIP AND MICROPARTICLE MEASURING APPARATUS

There is provided a microchip. The microchip comprises a substrate including a flow channel configured to convey a fluid therein. The substrate comprises a first substrate layer, a second substrate layer laminated to the first substrate layer to create the flow channel, and a discharge part formed in only one of the first substrate layer or the second substrate layer. The discharge part includes an opening directed toward an end face of the substrate, and being configured to eject the fluid flowing through the flow channel. 1. A microchip comprising:a substrate including a flow channel configured to convey a fluid therein, the substrate comprising:a first substrate layer;a second substrate layer laminated to the first substrate layer to create the flow channel; anda discharge part formed in only one of the first substrate layer or the second substrate layer, the discharge part:including an opening directed toward an end face of the substrate; andbeing configured to eject the fluid flowing through the flow channel.2. The microchip of claim 1 , wherein the discharge part is configured to eject the fluid to a cavity.3. The microchip of claim 1 , wherein the discharge part comprises a quadrangle shape.4. The microchip of claim 1 , wherein the discharge part is bilaterally symmetrical in a direction perpendicular to the first and second substrate layers.5. The microchip of claim 1 , wherein the flow channel comprises a tapered portion.6. The microchip of claim 1 , wherein the flow channel comprises a first portion and a second portion claim 1 , wherein:the first portion of the flow channel is formed in the first substrate layer; andthe second portion of the flow channel is formed in the second substrate layer.7. The microchip of claim 6 , wherein:the flow channel comprises a first end distal to the discharge part and a second end proximate to the discharge part;the discharge part is formed in the second substrate layer; andthe first portion of the flow channel tapers as ...

Method and apparatus for processing sample material

Methods and systems for treating material with focused acoustic energy and/or ultraviolet radiation are described. Sample material may be provided to a single batch vessel or may flow in a continuous or intermittent fashion into/out of a processing chamber within which the material is exposed to focused acoustic energy and/or ultraviolet radiation. Exposure to focused acoustics in a flow through environment may enhance the effects of the radiation on the sample material. Further, in flow-through or single batch arrangements, sample material may be retained exterior to or within a processing chamber by one or more retaining members (e.g., filter, valve, etc.) until a characteristic of the sample material reaches one or more particular criteria (e.g., physical, chemical, biological). Once the criteria of the sample material is attained (e.g., via further treatment), the material is then permitted to flow past the retaining member(s) and through the inlet or outlet of the chamber. Further, focused acoustic processing may be used to treat articles, such as the retaining member(s), rather than only sample material located within an internal processing volume of the chamber.

Fluidic Methods

The present disclosure relates to methods and devices that may be used to separate components from one or more samples.

MICROFLUIDIC CHIP

A microfluidic chip orients and isolates components in a sample fluid mixture by two-step focusing, where sheath fluids compress the sample fluid mixture in a sample input channel in one direction, such that the sample fluid mixture becomes a narrower stream bounded by the sheath fluids, and by having the sheath fluids compress the sample fluid mixture in a second direction further downstream, such that the components are compressed and oriented in a selected direction to pass through an interrogation chamber in single file formation for identification and separation by various methods. The isolation mechanism utilizes external, stacked piezoelectric actuator assemblies disposed on a microfluidic chip holder, or piezoelectric actuator assemblies on-chip, so that the actuator assemblies are triggered by an electronic signal to actuate jet chambers on either side of the sample input channel, to jet selected components in the sample input channel into one of the output channels. 143-. (canceled)44. An apparatus for identifying components in a fluid mixture , comprising: [ (i) a first intersection; and', '(ii) a second intersection;, '(a) a sample input channel disposed in a first structural layer, said sample input channel for receiving a fluid mixture containing a plurality of components, wherein said sample input channel comprises, '(b) a first plurality of sheath fluid channels disposed in said first structural layer, wherein said first plurality of sheath fluid channels intersect said sample input channel at said first intersection;', '(c) a second plurality of sheath fluid channels disposed in a second structural layer, wherein said second plurality of sheath fluid channels intersect said sample input channel at said second intersection; and', '(d) a plurality of output channels fluidly connected to and branching from said sample input channel, wherein each of said plurality of output channels is disposed between a pair of recessed portions at an end of said first ...

Monolithic carrier structure for digital dispensing

A digital dispense apparatus comprising a plurality of fluid dispense devices, at least one reservoir connected to the plurality of fluid dispense devices to deliver fluid to the plurality of fluid dispense devices, at least one contact pad array, and a single monolithic carrier structure.

SYSTEM AND METHOD FOR THE ACOUSTIC LOADING OF AN ANALYTICAL INSTRUMENT USING A CONTINUOUS FLOW SAMPLING PROBE

A system and method are provided for loading a sample into an analytical instrument using acoustic droplet ejection (“ADE”) in combination with a continuous flow sampling probe. An acoustic droplet ejector is used to eject small droplets of a fluid sample containing an analyte into the sampling tip of a continuous flow sampling probe, where the acoustically ejected droplet combines with a continuous, circulating flow stream of solvent within the flow probe. Fluid circulation within the probe transports the sample through a sample transport capillary to an outlet that directs the analyte away from the probe to an analytical instrument, e.g., a device that detects the presence, concentration quantity, and/or identity of the analyte. When the analytical instrument is a mass spectrometer or other type of device requiring the analyte to be in ionized form, the exiting droplets pass through an ionization region, e.g., an electrospray ion source, prior to entering the mass spectrometer or other analytical instrument. The method employs active flow control and enables real-time kinetic measurements. 155-. (canceled)56. A system for transporting a fluid sample to an analytical instrument , comprising:a continuous flow sampling probe;an acoustic ejector for successively ejecting discrete fluid sample droplets from a fluid sample into a sampling tip of the sampling probe; anda fluid handling system operative to:supply a flow of solvent from a solvent source to the sampling tip,dilute the fluid sample droplets with the solvent to form an analyte-solvent dilution, andtransport the analyte-solvent dilution to a sample outlet to be directed to the analytical instrument.57. The system of further comprising:a positioning component operative to position the acoustic ejector in acoustic coupling relationship with a reservoir housing including a reservoir containing the fluid sample.58. The system of claim 57 , wherein the positioning component comprises a movable stage for moving the ...

ACTUATION OF PARALLEL MICROFLUIDIC ARRAYS

An improved actuator for use in a microfluidic particle sorting system utilizes a staggered packing scheme for a plurality of actuators used to selectively deflect a particle in an associated sorting channel from a stream of channels. An actuator block may be provided for housing a two-dimensional array of actuators, each configured to align with an actuation port in an associated sorting chip containing a plurality of sorting channels. The actuator block may include a built-in stressing means to pre-stress each actuator housed by the block. An actuator comprising a piezo-electric stack may employ contact-based electrical connection rather than soldered wires to improve packing density. The actuator may be an external actuator. That is, the external actuator is external to the substrate in which the sorting channels are formed. 1. A particle processing assembly for sorting individual particles on a particle-by-particle basis from a stream of particles , the particle processing assembly comprising:a microfluidic chip including at least one microsorter having a switching region and a microfluidic channel formed in the microfluidic chip fluidically coupled to a sample input a keep output, a waste output, and the switching region,wherein the switching region interfaces with at least one actuator external to the microfluidic chip, andwherein the switching region, when actuated by the actuator upon detection of a predetermined characteristic of a selected particle, directs a pressure pulse across the microfluidic channel to deflect the selected particle from the stream of particles.2. The particle processing assembly of claim 1 , further comprising:a cartridge having fluid contact surfaces including a sample chamber, a keep chamber and a waste chamber, wherein the sample chamber of the cartridge is in fluid communication with the sample input of the microfluidic channel, wherein the keep chamber of the cartridge is in fluid communication with the keep output of the ...

NUCLEIC ACID SEPARATION

An example system includes an input channel to flow nucleic segments therethrough, a mixing portion coupled to the input channel, a separation chamber in fluid communication with the second end of the input channel, at least two output channels coupled to the chamber, and an integrated pump to facilitate flow through the separation chamber. The mixing portion is to include at least two different categories of beads having different sizes from each other and having a probe to attach to a corresponding nucleic acid segment. The separation chamber has a passive separation structure including an array of columns spaced apart to facilitate separation of the different categories of beads and attached corresponding nucleic acid segment into at least two flow paths based on a size of the category of the beads. Each output channel is to receive separated categories of beads and attached nucleic acid segments. 1. A system , comprising:an input channel having a first end and a second end to flow nucleic segments therethrough;a mixing portion coupled to the input channel, the mixing portion to include at least two different categories of beads, the different categories of beads having different sizes from each other, each category of beads having a probe to attach to a corresponding nucleic acid segment;a separation chamber in fluid communication with the second end of the input channel, the separation chamber having a passive separation structure, the passive separation structure including an array of columns spaced apart to facilitate separation of the different categories of beads and attached corresponding nucleic acid segment into at least two flow paths based on a size of the category of the beads;at least two output channels, each output channel coupled to the chamber to receive separated categories of beads and attached nucleic acid segments; andan integrated pump to facilitate flow through the separation chamber, the integrated pump being positioned within at least one ...

ELECTRONIC CONTROL OF FLUIDIC SPECIES

Various aspects of the present invention relate to the control and manipulation of fluidic species, for example, in microfluidic systems. In one aspect, the invention relates to systems and methods for making droplets of fluid surrounded by a liquid, using, for example, electric fields, mechanical alterations, the addition of an intervening fluid, etc. In some cases, the droplets may each have a substantially uniform number of entities therein. For example, 95% or more of the droplets may each contain the same number of entities of a particular species. In another aspect, the invention relates to systems and methods for dividing a fluidic droplet into two droplets, for example, through charge and/or dipole interactions with an electric field. The invention also relates to systems and methods for fusing droplets according to another aspect of the invention, for example, through charge and/or dipole interactions. In some cases, the fusion of the droplets may initiate or determine a reaction. In a related aspect of the invention, systems and methods for allowing fluid mixing within droplets to occur are also provided. In still another aspect, the invention relates to systems and methods for sorting droplets, e.g., by causing droplets to move to certain regions within a fluidic system. Examples include using electrical interactions (e.g., charges, dipoles, etc.) or mechanical systems (e.g., fluid displacement) to sort the droplets. In some cases, the fluidic droplets can be sorted at relatively high rates, e.g., at about 10 droplets per second or more. Another aspect of the invention provides the ability to determine droplets, or a component thereof, for example, using fluorescence and/or other optical techniques (e.g., microscopy), or electric sensing techniques such as dielectric sensing. 1273-. (canceled)274. A method of sorting droplets in a microfluidic system , the method comprising:providing a series of droplets of fluid surrounded by a liquid and flowing in a ...

SERIAL CELLULAR ANALYTICS

In one example in accordance with the present disclosure, a cellular analytic system is described. The cellular analytic system includes a series of analytic devices. Each analytic device includes 1) a separator to separate a cellular particle from a surrounding fluid, 2) an analyzer coupled to a first outlet of the separator to analyze the surrounding fluid, and 3) at least one lysing device coupled to at least a second outlet of the separator to rupture a membrane of the cellular particle. An outlet of the lysing device is fluidly coupled to a separator of a downstream analytic device. 1. A cellular analytic system , comprising: a separator to separate a cellular particle from a surrounding fluid;', 'an analyzer coupled to a first outlet of the separator to analyze the surrounding fluid; and', 'at least one lysing device coupled to at least a second outlet of the separator to rupture a membrane of the cellular particle, wherein an outlet of a lysing device is fluidly coupled to a separator of a downstream analytic device., 'a series of analytic devices, each analytic device comprising2. The system of claim 1 , wherein the separator comprises a dielectrophoresis separator.3. The system of claim 1 , wherein the at least one lysing device comprises:a lysing chamber to receive the cellular particle;at least one lysing element to rupture a cellular particle membrane; andat least one pair of electrodes disposed in the lysing chamber to detect a level of conductivity in the lysing chamber.4. The system of claim 3 , wherein the system further comprises a controller to determine when the cellular particle membrane has ruptured based on detected levels of conductivity within the lysing chamber.5. The system of claim 4 , wherein the at least one lysing device further comprises a return pump to re-direct the cellular particle to the lysing chamber when the cellular particle membrane is not sufficiently lysed.6. The system of claim 5 , wherein the return pump re-directs the ...

SYSTEM AND METHOD FOR AMPLIFYING NUCLEIC ACIDS FROM SINGLE CELLS

The present invention relates to a system for amplification of polynucleotides from a predefined number of single cells. The system comprise a device (or part) providing the predefined number of single cells, at a previously defined inlet site (or orifice) of a cartridge (microfluidic device), and the cartridge itself. The invention further relates to a method for amplification of polynucleotides from the one or more single cells using the system to provide an emulsion of aqueous droplets wherein the nucleic acid amplification occurs. Furthermore, the present invention relates to a kit comprising a plurality of microfluidic devices and a plurality of fluids configured for use with the system and the method. 1. A system for amplification of polynucleotides from a predefined number of single cells comprising a device (or part) providing a predefined number of single cells , at an inlet site (or orifice) of a cartridge (or microfluidic device) , and the cartridge ,said device provides a flow of single localized cells which ejects from the device, one cell at a time, and deposits them at a previously defined inlet site (or orifice) of the cartridge (or microfluidic device),said cartridge comprises one or more groups of containers,wherein each group of containers comprise a supply container, defining a supply cavity and comprising a primary orifice (or inlet site),an emulsification unit and a collection container,each group of containers comprise a plurality of fluid conduits that provide for fluid communication between the primary orifice, the emulsification unit and the collection container, and between a secondary orifice, the emulsification unit and the collection container.2. The system according to claim 1 , wherein the device claim 1 , providing single localized volumes claim 1 , provides volumes claim 1 , each of which comprising a single cell claim 1 , at an inlet site of the cartridge.3. The system according to claim 1 , wherein the device claim 1 , providing ...

MICROFLUIDIC DEVICES

According to an example, a microfluidic device may include a transport channel having an inlet and an outlet and a plurality of pump loops extending along the transport channel. Each of the plurality of pump loops may include a first branch, a second branch, and a connecting section connecting the first branch and the second branch. The first branch may include a first opening and the second branch may include a second opening, in which the first opening and the second opening are in direct fluid communication with the transport channel. The pump loops may also each include an actuator positioned in the first branch, in which the actuators in the pump loops are to be activated to induce a traveling wave that is to transport the fluid through the transport channel from the inlet to the outlet. 1. A microfluidic device comprising:a transport channel having an inlet and an outlet; a first branch, a second branch, and a connecting section connecting the first branch and the second branch, wherein the first branch includes a first opening and the second branch includes a second opening, and wherein the first opening and the second opening are in direct fluid communication with the transport channel; and', 'an actuator positioned in the first branch, and, 'a plurality of pump loops extending along the transport channel, wherein each of the plurality of pump loops includeswherein the actuators in the plurality of pump loops are to be activated to induce a traveling wave that is to transport the fluid through the transport channel from the inlet to the outlet.2. The microfluidic device according to claim 1 , wherein the plurality of pump loops is enclosed except for the first opening and the second opening.3. The microfluidic device according to claim 1 , wherein each of the plurality of pump loops does not include a nozzle.4. The microfluidic device according to claim 1 , wherein the actuators in the plurality of pump loops are grouped into respective primitives claim 1 , ...

EJECTION SUBASSEMBLY PITCHES TO MATCH NANOWELL SPACING

In one example in accordance with the present disclosure, a fluidic die is described. The fluidic die includes a plurality of ejection subassemblies. Each ejection subassembly includes an ejection chamber to hold a volume of fluid and an opening through which the volume of fluid is ejected via a fluid actuator. A pitch of the ejection subassemblies aligns with a spatial arrangement of nanowells in an array of nanowells on a substrate. 1. A fluidic die comprising: an ejection chamber to hold a volume of fluid; and', 'an opening through which the volume of fluid is ejected via a fluid actuator;, 'a plurality of ejection subassemblies, wherein each ejection subassembly compriseswherein a pitch of the plurality of ejection subassemblies matches a spatial arrangement of nanowells of an array of nanowells on a substrate.2. The fluidic die of claim 1 , wherein the pitch of the ejection subassemblies is such that each ejection subassembly ejects fluid into one of the nanowells of the array of nanowells.3. The fluidic die of claim 1 , wherein the plurality of ejection subassemblies simultaneously eject fluid into corresponding nanowells of the array of nanowells.4. The fluidic die of claim 3 , wherein a first ejection subassembly ejects fluid into one well and a second ejection subassembly ejects fluid into a second well simultaneously.5. The fluidic die of claim 1 , wherein:the fluidic die is one of a plurality of fluidic die which are arranged together; andeach of the ejection subassemblies of the plurality of fluidic die eject fluid into the array of nanowells.6. The fluidic die of claim 5 , wherein the ejection subassemblies address a group of nanowells that is different from a group of nanowells addressed by other fluidic die of the plurality of fluidic die.7. A fluid ejection system comprising:a substrate stage to hold a substrate that comprises an array of nanowells; and each ejection subassembly comprises an ejection chamber to hold a volume of fluid and an opening ...

Devices, systems, and methods for generating droplets

Devices, systems, and their methods of use, for generating droplets are provided. The devices, systems, and methods may include transporting a first liquid through an outlet of a channel and causing relative motion of the outlet and an interface of a second liquid to produce droplets of the first liquid in the second liquid. The devices, systems, and methods may also include illuminating a portion of the liquid as the liquid exits from an outlet. The invention also provides methods, devices, and systems for changing the size of a droplet and for eliminating a droplet from a plurality of droplets.

Actuation of microchannels for optimized acoustophoresis

The systems and methods of the present disclosure provide techniques for the design and use of an intermediate or transitional plate or block designed to couple acoustic energy at a given frequency from a transducer, such as a piezoelectric transducer, to one or more acoustophoretic devices, such as microfluidic channels, such that driving the chip occurs with a controlled wavelength and symmetry. Such techniques provide improved efficiency when driving a single acoustophoretic device, or for multiple acoustophoretic devices to be operated in concert from a single transducer, and therefore without complex electronics. Additionally, the techniques described herein allow for relaxed design constraints when considering transducer selection and fabrication, instead transferring design constraints to the more easily customized actuation plate.

Vibrating a dispense head to move fluid

In an example implementation, a method of dispensing fluid from a fluid dispensing device, includes receiving a dispense head at a receiving station, and receiving a notification that a supply slot in the dispense head has been filled with fluid. The method includes vibrating the dispense head to move fluid through a microfluidic channel from the supply slot into an ejection chamber of the dispense head, and providing a dispense signal to cause an ejection mechanism disposed within the chamber to eject an amount of the fluid from the dispense head.

MICROPARTICLE SORTING MICROCHIP AND MICROPARTICLE SORTING APPARATUS

A microparticle sorting microchip for a flow cytometer is provided to enable sorting of microparticles at higher speed, higher purity, and higher acquisition rate. The microparticle sorting microchip includes a main channel through which a microparticle-containing fluid flows, a trap channel coaxially communicating with the main channel, a trap chamber communicating with the trap channel, and a gate channel intersecting the trap channel. The trap channel has an opening intersecting the gate channel. The trap channel has a smaller cross-sectional area upstream of the opening than downstream of the opening along a direction in which the microparticle-containing fluid flows. 1. A microparticle sorting microchip comprising:a main channel through which a microparticle-containing fluid flows;a trap channel coaxially communicating with the main channel;a trap chamber communicating with the trap channel; anda gate channel intersecting the trap channel, whereinthe trap channel has an opening portion intersecting the gate channel, andan upstream cross-sectional area of the opening portion is smaller than a downstream cross-sectional area of the opening portion, along a direction in which the microparticle-containing fluid flows.2. The microparticle sorting microchip of claim 1 , whereinthe gate channel includes plural gate channels, and the plural gate channels are connected to an upstream end of the downstream-stage trap channel so that the plural gate channels are symmetrical to each other with respect to a center of a flow of the microparticle-containing fluid from an opening at a downstream end of the upstream-stage trap channel.3. The microparticle sorting microchip of claim 1 , whereinthe gate channel is configured to allow a gate flow fluid to always flow at a constant flow rate.4. The microparticle sorting microchip of claim 3 , whereinin the downstream-stage trap channel, the gate flow fluid is branched to flow from the gate channel in a direction toward the upstream ...

METHODS AND MICROFLUIDIC DEVICES FOR THE MANIPULATION OF DROPLETS IN HIGH FIDELITY POLYNUCLEOTIDE ASSEMBLY

Methods and devices are provided for manipulating droplets on a support using surface tension properties, moving the droplets along a predetermined path and merging two droplets together enabling a number of chemical reactions. Disclosed are methods for controlling the droplets volumes. Disclosed are methods and devices for synthesizing at least one oligonucleotide having a predefined sequence. Disclosed are methods and devices for synthesizing and/or assembling at least one polynucleotide product having a predefined sequence from a plurality of different oligonucleotides having a predefined sequence. In exemplary embodiments, the methods involve synthesis and/or amplification of different oligonucleotides immobilized on a solid support, release of synthesized/amplified oligonucleotides in solution to form droplets, recognition and removal of error-containing oligonu-cleotides, moving or combining two droplets to allow hybridization and/or ligation between two different oligonucleotides, and further chain extension reaction following hybridization and/or ligation to hierarchically generate desired length of polynucleotide products. 1. A method for preparing a plurality of oligonucleotides on a support , the method comprising:a. providing a support comprising a plurality of surface-bound single-stranded oligonucleotides contained within one or more droplets of a predefined volume of solution;b. exposing the plurality of surface-bound oligonucleotides to conditions suitable for a template-dependent synthesis reaction, thereby producing a plurality of complementary oligonucleotides; andc. adjusting or substantially maintaining the volume of the one or more droplets of solution.2. The method of wherein the step of adjusting or substantially maintaining the volume of the one or more droplets of solution comprises maintaining the droplets under conditions that substantially limit solvent evaporation.3. The method of wherein the plurality of surface-bound oligonucleotides ...

APPARATUS FOR FORMING LIQUID FLOW INCLUDING BIOLOGICAL PARTICLES, AND TREATMENT APPARATUS

There are provided an apparatus capable of treating biological particles in a sterile state, and a treatment apparatus. An apparatus for forming a liquid flow including biological particles includes: a chamber member; a sample liquid supply portion; a sheath liquid supply portion; and a vibrating electrode member. The chamber member includes a chamber and a flow cell extending from an interior to an exterior of the chamber. The sample liquid supply portion is configured to supply a sample liquid including the biological particles into the chamber. The sheath liquid supply portion is configured to supply a sheath liquid into the chamber. The vibrating electrode member extends from the interior to the exterior of the chamber, is made of an electrically conductive material, and is configured to be capable of supplying an electric charge to the sheath liquid and the sample liquid in the chamber and propagating an ultrasonic vibration. 1. An apparatus for forming a liquid flow including biological particles , the apparatus comprising:a chamber member including a chamber and a flow cell extending from an interior to an exterior of the chamber;a sample liquid supply portion configured to supply a sample liquid including the biological particles into the chamber;a sheath liquid supply portion configured to supply a sheath liquid into the chamber; anda vibrating electrode member extending from the interior to the exterior of the chamber, made of an electrically conductive material, and configured to be capable of supplying an electric charge to the sheath liquid and the sample liquid in the chamber and propagating an ultrasonic vibration.2. The apparatus according to claim 1 , whereinan end face of the vibrating electrode member exposed to the interior of the chamber is disposed so as to be continuous with a portion of an inner surface of the chamber adjacent to the end face.3. A treatment apparatus comprising:a sterilization treatment portion including a sterilization ...

ACOUSTO-THERMAL SHIFT ASSAY FOR LABEL-FREE PROTEIN ANALYSIS

Thermal shift assays (TSAs) have been extensively used to study thermodynamics of proteins and provide an efficient means to assess protein-ligand binding or protein-protein interaction. However, existing TSAs have limitations such as time consuming, labor intensive, or low sensitivity. Here, an acousto-thermal shift assay (A-TSA) is disclosed and is believed to be the first ultrasound enabled TSA which can provide a real-time analysis of protein thermodynamic stability. A-TSA couples unique acoustic mechanisms to achieve protein unfolding, concentration, and measurement on a single microfluidic chip within minutes. Compared to conventional TSA methods, A-TSA provides an ultra-fast (at least 30 times faster), highly sensitive (7-34 folds higher), and label -free monitoring of protein-ligand interactions and protein stability finding applications for protein analysis in biology, medicine and fast diagnosis. 1. A method , comprising: i) an acousto-thermal device comprising a surface acoustic wave source and at least one microfluidic channel or chamber; and', 'ii) a sample comprising at least one protein;, 'a) providingb) introducing said sample into said at least one microfluidic channel or chamber;c) controlling the temperature of the said sample with said surface acoustic wave source to a plurality of precise temperatures within said at least one microfluidic channel or chamber under conditions that create a precipitated protein; andd) aggregating said precipitated protein with said surface acoustic wave into a pattern.2. The method of claim 1 , wherein said pattern comprises parallel lines or arrays.3. The method of claim 1 , wherein said aggregating increases a local concentration of said precipitated protein.4. The method of claim 1 , wherein said aggregating is performed simultaneously with said protein precipitation.5. The method of claim 1 , further comprising measuring protein gray intensity.6. The method of claim 5 , wherein said protein gray intensity ...

MONOLITHIC CARRIER STRUCTURE INCLUDING FLUID ROUTING FOR DIGITAL DISPENSING

A method of manufacturing a digital dispense apparatus includes molding a monolithic carrier structure, forming cut outs into a planar top surface of the monolithic carrier structure, the cut outs including a reservoir extending into part of a thickness of the monolithic carrier structure and fluid routing to connect the reservoir with a fluid dispense device, and overmolding the fluid dispense device into the monolithic carrier structure on a side of the monolithic carrier structure that is opposite to the reservoir to fluidically connect to the fluid routing. 1. A method of manufacturing a digital dispense apparatus , comprising:molding a monolithic carrier structure;forming cut outs into a planar top surface of the monolithic carrier structure, the cut outs including a reservoir extending into part of a thickness of the monolithic carrier structure and fluid routing to connect the reservoir with a fluid dispense device; andovermolding the fluid dispense device into the monolithic carrier structure on a side of the monolithic carrier structure that is opposite to the reservoir to fluidically connect to the fluid routing.2. The method of claim 1 , comprises:overmolding a plurality of fluid dispense devices in an array in a plane, wherein the plurality of fluid dispense devices includes the fluid dispense device; andshaping the fluid routing to cover a distance of the plurality of fluid dispense devices.3. The method of claim 2 , comprising:forming a contact pad array next to the reservoir;forming electrical routing on the monolithic carrier structure; andforming through mold vias (TMVs) through the monolithic carrier structure to connect the contact pad array to the fluid dispense device.4. The method of claim 1 , wherein forming the cut outs comprises:forming the reservoir that includes a first surface recessed relative to the planar top surface of the monolithic carrier structure and a plurality of side walls defined by the monolithic carrier structure; ...

MICROFLUIDIC APPARATUSES

According to an example, a microfluidic apparatus may include a channel, a foyer, in which the foyer is in fluid communication with the channel and in which the channel has a smaller width than the foyer, a sensor to sense a property of a fluid passing through the channel, a nozzle in fluid communication with the foyer, and an actuator positioned in line with the nozzle. The microfluidic apparatus may also include a controller to determine whether the sensed property of the fluid meets a predetermined condition and to perform a predefined action in response to the sensed property of the fluid meeting the predetermined condition. 1. A microfluidic apparatus comprising:a channel;a foyer, wherein the foyer is in fluid communication with the channel and wherein the channel has a smaller width than the foyer;a sensor to sense a property of a fluid passing through the channel;a nozzle in fluid communication with the foyer;an actuator positioned in line with the nozzle; anda controller to determine whether the sensed property of the fluid meets a predetermined condition and to perform a predefined action in response to the sensed property of the fluid meeting the predetermined condition.2. The microfluidic apparatus according to claim 1 , wherein the predefined action includes at least one of changing a dispensing location of the fluid contained in the foyer claim 1 , changing an order in which the fluid contained in the foyer and another fluid contained in another foyer are dispensed claim 1 , changing a quantity of fluid dispensed into a well claim 1 , dispensing a second fluid into a well into which the fluid was dispensed claim 1 , and dispensing the second fluid at a specific time following a determination that the sensed property of the fluid meets the predetermined condition.3. The microfluidic apparatus according to claim 1 , wherein the controller is to perform the predefined action by modifying a dispense protocol applied to the fluid contained in the foyer.4. ...

Methods and devices for high fidelity polynucleotide synthesis

Disclosed are methods for synthesizing and/or assembling at least one polynucleotide product having a predefined sequence from a plurality of different oligonucleotides. In exemplary embodiments, the methods involve synthesis and/or amplification of different oligonucleotides immobilized on a solid support, release of synthesized/amplified oligonucleotides in solution to form droplets, recognition and removal of error-containing oligonucleotides, moving or combining two droplets to allow hybridization and/or ligation between two different oligonucleotides, and further chain extension reaction following hybridization and/or ligation to hierarchically generate desired length of polynucleotide products.

PARTICLE MANIPULATION

The present invention relates to the manipulation and, more particularly, to the sorting of particles. Still more particularly, the present invention relates to the use of acoustic waves, including surface acoustic waves, for the manipulation and sorting of particles. In an example, the present invention may be used for the sorting of cells. In an aspect of the invention, there is provided a device for manipulating a particle in a fluid suspension, the device comprising: (a) a substrate having a surface; (b) an acoustic source configured to generate and deliver an acoustic wave within a region of the substrate surface; and (c) a pillar disposed on the surface of the substrate and the pillar is configured to receive a channel, the channel configured to receive the fluid suspension, wherein the acoustic wave delivered within the region of the substrate surface is delivered to the fluid suspension in the channel through the pillar. A method using the device of the invention is also disclosed. 1. A device for manipulating a particle in a fluid suspension , the device comprising:(a) a substrate having a surface;(b) an acoustic source configured to generate and deliver an acoustic wave within a region of the substrate surface; and(c) the surface of the substrate is configured to receive a pillar and the pillar is configured to receive a channel, the channel configured to receive the fluid suspension,wherein the acoustic wave delivered within the region of the substrate surface is delivered to the fluid suspension in the channel through the pillar.2. The device according to claim 1 , wherein the pillar is configured to be detachably attached to the surface of the substrate.3. The device according to claim 1 , wherein the pillar forms a wall or part of a wall of the channel.4. The device according to claim 1 , wherein the pillar is made of a material suitable for conducting an acoustic wave claim 1 , the material is selected from the group consisting of: glass claim 1 , ...

Methods and devices for sorting droplets and particles

Devices, systems, and their methods of use, for sorting droplets or particles are provided. A source of acoustic energy can be employed to sort droplets or particles of a desired and predictable property.

MICROFLUIDIC DEVICE FOR FLUID MIXTURE

Examples include microfluidic devices. Example microfluidic devices comprise a first microfluidic channel, a second microfluidic channel, and microfluidic output channel fluidly coupled to the first microfluidic channel and the second microfluidic channel via a fluid junction. The example device comprises a fluid actuator disposed in the microfluidic output channel to actuate to thereby pump a first fluid and a second fluid into the microfluidic output channel. 1. A microfluidic device comprising:a first microfluidic channel to convey a first fluid;a second microfluidic channel to convey a second fluid;a microfluidic output channel fluidly coupled to the first microfluidic channel and the second microfluidic channel at a fluid input junction; anda first fluid actuator disposed in the microfluidic output channel, the first fluid actuator to actuate to thereby pump the first fluid into the microfluidic output channel from the first microfluidic channel and to thereby pump the second fluid into the microfluidic output channel from the second microfluidic channel.2. The microfluidic device of claim 1 , further comprising:a third microfluidic channel fluidly coupled to the microfluidic output channel at a fluid output junction; anda fourth microfluidic channel coupled to the microfluidic output channel at the fluid output junction.3. The microfluidic device of claim 1 , wherein the first fluid actuator is disposed in the microfluidic output channel such that actuation of the first fluid actuator is to pump a first volume of the first fluid and a second volume of the second fluid into the microfluidic output channel.4. The microfluidic device of further comprising:a second fluidic actuator disposed in the microfluidic output channel.5. The microfluidic device of claim 4 , wherein the second fluid actuator is to actuate to thereby mix the first fluid and the second fluid in the microfluidic output channel.6. The microfluidic device of claim 4 , wherein the second fluid ...

MICROFLUIDIC ULTRASONIC PARTICLE SEPARATORS WITH ENGINEERED NODE LOCATIONS AND GEOMETRIES

An ultrasonic microfluidic system includes a separation channel for conveying a sample fluid containing small particles and large particles, flowing substantially parallel, adjacent to a recovery fluid, with which it is in contact. An acoustic transducer produces an ultrasound standing wave, that generates a pressure field having at least one node of minimum pressure amplitude. An acoustic extension structure is located proximate to said separation channel for positioning said acoustic node off center in said acoustic area and concentrating the large particles in said recovery fluid stream. 1. An ultrasonic microfluidic apparatus for separating small particles from large particles contained in a sample fluid , comprising:a sample input channel for channeling the sample fluid containing the small particles and the large particles;a recovery fluid input channel containing recovery fluid, routing said recovery fluid to flow substantially parallel and adjacent to the sample fluid, wherein said recovery fluid contacts the sample fluid;an acoustic transducer that producesan acoustic standing wave, that generates a pressure field having at least one node of minimum sound pressure amplitude; andan acoustic extension structure located proximate the sample fluid and said recovery fluid that positions said at least one acoustic node in said recovery fluid concentrating the large particles in said recovery fluid wherein said acoustic extension structure includes a gel located proximate the sample fluid and said recovery fluid positioning said at least one acoustic node in said recovery fluid concentrating the large particles in said recovery fluid.2. The ultrasonic microfluidic apparatus for separating small particles from large particles contained in a sample fluid of wherein the sample fluid and said recovery fluid are positioned to form a separation channel claim 1 , and wherein said at least one node of minimum sound pressure amplitude is positioned off center in said ...

ENHANCED CELL/BEAD ENCAPSULATION METHODS AND APPARATUSES

A microfluidic droplet system that employs flow-focusing methods to generate droplets of desired sizes for encapsulating particles, cells or beads. The microfluidic droplet system can comprise an air cavity that can be vibrated to reduce trapping of cells or beads in flow-focusing regions of the microfluidic droplet systems thereby increasing the encapsulation efficiency of the cells/beads. 1. A microfluidic device , comprising:a first fluid channel configured to transport a first fluid;a second fluid channel configured to transport a second fluid;an output fluid channel disposed downstream from the first and the second fluid channels; and an orifice disposed to output the first and the second fluids transported through the first and the second fluid channels into the output fluid channel; and', 'an air cavity placed upstream from the orifice., 'a flow-focusing region, the first and the second fluid channels terminating in the flow-focusing region, the flow-focusing region comprising2. The microfluidic device , further comprising a piezo-electric transducer configured to vibrate the air cavity.3. A method of encapsulating a solid sample in a droplet , the method comprising:flowing a first fluid through a first fluid channel, the first fluid including the cell/bead;flowing a second fluid through a second fluid channel;forcing the first and the second fluids through an orifice into an output fluid channel; andmodulating a fluid flow parameter in a zone disposed upstream from the orifice,wherein a flow rate of the second fluid is configured to generate droplets of the first fluid of a desired size, andwherein the fluid flow parameter is configured to achieve a desired encapsulation efficiency.4. The method of claim 3 , wherein a piezo-electric transducer is configured to vibrate the air cavity.5. The method of claim 3 , wherein the solid sample comprises a cell.6. The method of claim 3 , wherein the solid sample comprises a bead including an organic material.7. The ...

Dispensing apparatus, liquid dispensing method, and cell dispensing method

A dispensing apparatus, a liquid dispensing method, and a cell dispensing method which can dispense liquid with very high accuracy. A dispensing apparatus includes a glass pipette, a tubular elastic member, a rod-shaped member, and a piezoelectric element actuator. A portion of the tubular elastic member located adjacent to a first open end thereof covers a portion of the glass pipette located adjacent to an opening portion thereof. A portion of the tubular elastic member located adjacent to a second open end thereof covers at least a forward end portion of the rod-shaped member. When the piezoelectric element actuator pushes the rod-shaped member toward the opening portion of the glass pipette, the tubular elastic member deforms such that the volume of the internal space of the tubular elastic member decreases.

LIQUID DISCHARGE DEVICE, SENSOR MANUFACTURING DEVICE HAVING LIQUID DISCHARGE DEVICE, AND CELL CULTURE DEVICE HAVING LIQUID DISCHARGE DEVICE

A liquid discharge device includes a nozzle from which liquid drops are discharged, a fixing part for fixing the nozzle, and a driving part generating displacement, in which the nozzle is fixed to a first holding part in the driving part and a second holding part in the fixing part. 1. A liquid discharge device comprising:a nozzle from which liquid drops are discharged;a fixing part for fixing the nozzle; anda driving part generating displacement,wherein the nozzle is fixed to a first holding part in the driving part and a second holding part in the fixing part.2. The liquid discharge device according to claim 1 ,wherein the first holding part and the nozzle as well as the second holding part and the nozzle are joined by a fitting method.3. The liquid discharge device according to claim 1 ,wherein a thickness of the fixing part is thinner than a thickness of the driving part.4. The liquid discharge device according to claim 1 ,wherein the nozzle is combined with the first holding part and the second holding part at a lower surface of the liquid discharge device.5. The liquid discharge device according to claim 1 ,wherein the second holding part is provided at an arm part passing above the nozzle.6. The liquid discharge device according to claim 1 ,wherein the arm part forms an opening.7. The liquid discharge device according to claim 1 ,wherein a positioning mechanism for attaching the liquid discharge device is provided on an upper surface of the liquid discharge device.8. The liquid discharge device according to claim 1 ,wherein the second holding part, the driving mechanism, and the first holding part are formed in the same plane.9. The liquid discharge device according to claim 1 ,wherein the driving mechanism is a piezoelectric element.10. A liquid discharge device comprising:{'claim-ref': {'@idref': 'CLM-00001', 'claim 1'}, 'a plurality of liquid discharge devices according to ,'}wherein the plural liquid discharge devices are arranged on one plate at ...

SYSTEMS AND METHODS FOR TAGGING AND ACOUSTICALLY CHARACTERIZING CONTAINERS

Embodiments of the present invention provide systems and methods for tagging and acoustically characterizing containers. 111.-. (canceled)12. A method of characterizing a container configured to hold a fluid , the method comprising: at least one vertical sidewall; and', 'a bottom coupled to the at least one vertical sidewall, the bottom being configured to receive an acoustic signal, the bottom including a plurality of recesses, grooves, or protrusions thereon or therein;, 'providing the container, the container comprisingtransmitting an acoustic signal through the bottom, the plurality of recesses, grooves, or protrusions providing a plurality of times of flight of the acoustic signal through the bottom;receiving a reflection of the transmitted acoustic signal; andcharacterizing an acoustic impedance of the container based on the reflection.13. The method of claim 12 , further comprising:retrieving from a computer-readable medium a value characterizing a thickness of the bottom based on the reflection; andwherein the characterizing the acoustic impedance of the container is based on the retrieved value characterizing the thickness of the bottom.14. The method of claim 12 , wherein a first subset of the plurality of recesses claim 12 , grooves claim 12 , or protrusions includes a first depth providing a first time of flight of the acoustic signal claim 12 , and wherein a second subset of the plurality of recesses claim 12 , grooves or protrusions includes a second depth providing a second time of flight of the acoustic signal claim 12 , the first depth being different from the second depth.15. The method of claim 12 , wherein a first one of the plurality of recesses claim 12 , grooves claim 12 , or protrusions includes a different length claim 12 , aspect ratio claim 12 , or depth than a second one of the plurality of recesses claim 12 , grooves claim 12 , or protrusions.16. (canceled)17. The method of claim 15 , wherein the plurality of recesses claim 15 , grooves ...

Method and Arrangement for Focusing Objects in a Flow

At least one embodiment relates to a focusing arrangement for focusing particles or cells in a flow. The arrangement includes at least one channel for guiding the flow. The channel includes (i) at least one particle confinement structure having particle flow boundaries and (ii) at least one acoustic confinement structure having acoustic field boundaries adapted for confining acoustic fields. The acoustic field boundaries may be different from the particle flow boundaries, and the at least one acoustic confinement structure may be arranged with regard to the channel to at least partially confine acoustic fields in the channel. 1. A focusing arrangement for focusing particles or cells in a flow , the focusing arrangement comprising:a channel for guiding the flow, wherein the channel comprises a particle confinement structure, and wherein the particle confinement structure comprises particle flow boundaries; andan acoustic confinement structure comprising acoustic field boundaries adapted for confining acoustic fields,wherein the acoustic field boundaries are different from the particle flow boundaries, and wherein the acoustic confinement structure is arranged with regard to the channel to confine acoustic fields, at least partially, in the channel.2. The focusing arrangement of claim 1 , wherein the acoustic confinement structure is a first acoustic confinement structure claim 1 , wherein the channel is a first channel that comprises the first acoustic confinement structure claim 1 , and wherein the focusing arrangement further comprises (i) a second channel and (ii) a second acoustic confinement structure adapted for confining acoustic fields claim 1 , at least partially claim 1 , in the second channel.3. The focusing arrangement of claim 2 , further comprising an acoustic barrier between the first and second acoustic confinement structures.4. The focusing arrangement of claim 3 , wherein the acoustic barrier is adapted to reflect a travelling acoustic wave.5. The ...

MICROFLUIDIC DEVICES WITH IMPEDANCE SETTING TO SET BACKPRESSURE

A microfluidic device may include a fluid channel defined in a substrate, an impedance sensor positioned within the fluid channel, and control logic. The control logic may force a current into the impedance sensor to sense an impedance at the location of the impedance sensor, the sensed impedance defining whether the fluid within the fluid channel is at the location of the impedance sensor, and instruct a pump device to apply a back pressure on the fluid to maintain the fluid upstream from the impedance sensor in response to a determination that the sensed impedance indicates that the fluid is located at the location of the impedance sensor. 1. A microfluidic device , comprising:a fluid channel defined in a substrate;an impedance sensor positioned within the fluid channel; and force a current into the impedance sensor to sense an impedance at the location of the impedance sensor, the sensed impedance defining whether the fluid within the fluid channel is at the location of the impedance sensor; and', 'instruct a pump device to apply a back pressure on the fluid to maintain the fluid upstream from the impedance sensor in response to a determination that the sensed impedance indicates that the fluid is located at the location of the impedance sensor., 'control logic to2. The microfluidic device of claim 1 , wherein the impedance sensor comprises:a first impedance sensor located at a first location within the fluid channel of the microfluidic device; anda second impedance sensor located at a second location within the fluid channel downstream relative to the first impedance sensor,wherein the control logic instructs the pump device to allow the fluid to move past the first impedance sensor but not past the second impedance sensor based on the detection of the fluid by the first impedance sensor and the second impedance sensor.3. The microfluidic device of claim 1 , wherein the impedance sensor comprises a single impedance sensor comprising a conductive plate with a ...

Conductivity-based lysis monitors

In one example in accordance with the present disclosure, a conductivity-based lysis monitor is described. The lysis monitoring device includes a lysing chamber to receive a cell to be lysed and at least one lysing device to rupture a cell membrane. At least one pair of electrodes are disposed in the lysing chamber to detect a level of conductivity in the lysing chamber. A controller of the device determines when the cell membrane has ruptured based on detected levels of conductivity in the lysing chamber.

Fluidic devices and methods of manufacturing the same

An example method includes providing a working stack having a first substrate layer, a second substrate layer, and a radiation-absorbing material disposed between the first and second substrate layers. The working stack includes a cavity therein having a designated liquid. A bonding interface is defined between the radiation-absorbing material and at least one of the first substrate layer or the second substrate layer. The bonding interface has a film of the designated liquid. The method also includes directing radiation onto the bonding interface to form a perimeter seal. The perimeter seal separates the cavity from an outer area of the bonding interface. The method also includes directing the radiation onto the outer area of the bonding interface to secure the first and second substrate layers together. The perimeter seal impedes an ingress of bubbles from the outer area into the cavity as the radiation is directed onto the outer area.

METHODS AND DEVICES FOR HIGH FIDELITY POLYNUCLEOTIDE SYNTHESIS

Disclosed are methods for synthesizing and/or assembling at least one polynucleotide product having a predefined sequence from a plurality of different oligonucleotides. In exemplary embodiments, the methods involve synthesis and/or amplification of different oligonucleotides immobilized on a solid support, release of synthesized/amplified oligonucleotides in solution to form droplets, recognition and removal of error-containing oligonucleotides, moving or combining two droplets to allow hybridization and/or ligation between two different oligonucleotides, and further chain extension reaction following hybridization and/or ligation to hierarchically generate desired length of polynucleotide products. 1. A method for assembling a polynucleotide having a predefined sequence from a plurality of different oligonucleotides , the method comprising:a) providing a plurality of single-stranded template oligonucleotides on a support, wherein each of the plurality of template oligonucleotides comprises a predefined sequence and includes a primer binding site;b) generating a complementary oligonucleotide for each of the plurality of template oligonucleotides by enzyme-catalyzed synthesis within a primary droplet, thereby producing a plurality of double-stranded oligonucleotides;c) releasing the complementary oligonucleotide from the double-stranded oligonucleotides into the primary droplet;d) combining at least a first and second primary droplets, thereby forming a secondary droplet, wherein the first primary droplet includes a released oligonucleotide that comprises a portion that is complementary to a portion of a released or template oligonucleotide from the second primary droplet; ande) exposing the secondary droplet to conditions suitable for hybridization and ligation, polymerase extension, or polymerase extension and ligation to assemble a double-stranded polynucleotide having a predefined sequence.2. The method of wherein the plurality of single-stranded template ...

CARTRIDGES, KITS, AND METHODS FOR AMPLIFICATION AND DETECTION OF ANALYTES

Devices, systems, and methods for detecting molecules of interest within a collected sample are described herein. In certain embodiments, self-contained sample analysis systems are disclosed, which include a reusable reader component, a disposable cartridge component, and a disposable sample collection component. The reader component may communicate with a remote computing device for the digital transmission of test protocols and test results. In various disclosed embodiments, the systems, components, and methods are configured to identify the presence, absence, and/or quantity of particular nucleic acids, proteins, or other analytes of interest, for example, in order to test for the presence of one or more pathogens or contaminants in a sample. 1. A sample analysis cartridge comprising:an input tunnel that extends from an aperture, the input tunnel configured to permit insertion of a sample collection device having a distal portion adapted to be exposed to a sample comprising a target nucleic acid;a reservoir configured to hold a fluid, the reservoir further configured to receive the sample from the sample collection device such that the sample is mixed with an effective amount of reagents for amplification of the target nucleic acid in the fluid of the reservoir to generate amplicons comprising signaling agents;an analysis channel configured to receive the fluid comprising the amplicons from the reservoir; anda sensor at least partially disposed in the analysis channel such that the amplicons indicative of at least one of a presence, absence, or quantity of the target nucleic acid in the sample localize over the sensor and the sensor senses signals indicative of at least one of the presence, absence, or quantity of the target nucleic acid in the sample based on reactions between a substrate and the signaling agents of the amplicons localized over the sensor.2. The sample analysis cartridge of claim 1 , further comprising reagent ball comprising the reagents for ...

DEVICES, SYSTEMS AND METHODS FOR EVALUATION OF HEMOSTASIS

Provided are devices, systems and methods for evaluation of hemostasis. In some embodiments, an apparatus is disclosed comprising a housing; a plurality of test chambers located in the housing, the plurality of test chambers including chambers configured for measurements via a system that interrogates one or more viscoelastic properties of test samples in the test chambers, wherein the one or more viscoelastic properties is used to characterize dynamics of coagulation and/or fibrinolysis. 173.-. (canceled)74. An apparatus comprising:a housing;a plurality of test chambers located in the housing, the plurality of test chambers including chambers configured for measurements via a system that interrogates one or more viscoelastic properties of test samples in the test chambers, wherein the one or more viscoelastic properties is used to characterize dynamics of coagulation and/or fibrinolysis, wherein the plurality of test chambers comprise a first test chamber and a second test chamber, each of the first test chamber and the second test chamber having reagents that interact with a test sample of blood received therein; anda fluid pathway comprising a plurality of channels, each defined at least in part by the housing, wherein the fluid pathway includes an inlet, defined at least in part by the housing, through which the test sample is introduced into the apparatus, wherein at least one channel of the fluid pathway is in communication with the inlet and with the first test chamber and a second test chamber to deliver a portion of the test sample to each of the first test chamber, wherein the at least one channel of the fluid pathway includes an inlet channel, a first channel, and a second channel, wherein the inlet channel is in communication with the inlet, and wherein the first channel is in communication with the inlet channel and at least with the first test chamber, and wherein the second channel is in communication with the inlet channel and at least with the ...

ULTRASOUND-BASED PATTERNING OF PARTICLES AND CELLS WITHIN FLUID MATRICES

Method of ultrasound-assisted 3D bioprinting includes depositing a bioink fluid matrix containing a suspension of cells into a chamber comprising two piezo transducers on opposing ends of the chamber. The method further includes vibrating the piezo transducers to generate longitudinal bulk acoustic waves within the bioink fluid matrix such that waves from opposing piezo transducers superimpose to form a standing bulk acoustic wave to drive the cells to cluster and align along one or more nodes or nodal planes formed within the bioink fluid matrix at points of intersection of the standing bulk acoustic wave. The nodes or nodal planes are spaced apart from each other by a distance equaling half a wavelength of the standing bulk acoustic wave. The nodes or nodal planes further mimic a contour of the vibrating surfaces of the piezo transducers. 1. A method of ultrasound-assisted 3D bioprinting , the method comprising:depositing a bioink fluid matrix containing a suspension of cells or particles into a chamber comprising a piezo transducer and a reflector positioned on opposing ends of the chamber;vibrating the piezo transducer to generate longitudinal bulk acoustic waves within the bioink fluid matrix such that waves emanating from the piezo transducer and reflected waves from the reflector superimpose to form a standing bulk acoustic wave; anddriving the cells or particles to cluster and align the cells or particles along one or more nodes or nodal planes formed within the bioink fluid matrix at points of intersection of the standing bulk acoustic wave to form a construct,wherein the nodal planes are spaced apart from each other by a distance equaling half a wavelength of the standing bulk acoustic wave,wherein the nodes or nodal planes mimic a contour of the vibrating surfaces of the piezo transducer or the reflector, or organize a pattern determined using computational modeling.2. The method of claim 1 , further comprising solidifying the bioink fluid matrix using ...

FLUID ANALYZER WITH MODULATION FOR LIQUIDS AND GASES

A fluid analyzer includes an optical source and an optical detector defining an optical beam path through an interrogation region of a fluid flow cell. Flow-control devices conduct analyte and reference fluids through a channel and the interrogation region, and manipulate fluid flow in response to control signals to move a fluid boundary separating the analyte and reference fluids across the interrogation region. A controller generates control signals to (1) cause the fluid boundary to be moved across the interrogation region accordingly, (2) sample an output signal from the optical detector at a first interval during which the interrogation region contains more analyte fluid than reference fluid and at a second interval during which the interrogation region contains more reference fluid than analyte fluid, and (3) determine from samples of the output signal a measurement value indicative of an optically measured characteristic of the analyte fluid. 1. A fluidic cell for use in analyzing a property of fluids , comprising:first and second cell windows, the first or second cell windows being transmissive to an optical beam generated by an optical source;a first flow channel for conducting a fluid within the fluidic cell, the first flow channel defined on one side by the first cell window and on a second side by the second cell window;an interrogation region within the first flow channel wherein the fluid interacts with the optical beam over a pathlength;an inlet channel for flowing fluids into the first flow channel and an outlet channel for flowing fluids out of the flow channel2. The fluidic cell of claim 1 , wherein the first and second cell window are positioned relative to each other such that different spatial positioning of the optical beam relative to the cell results in different pathlengths.3. The fluidic cell of claim 1 , wherein a surface of the first cell windows is coated with a reflective film reflective to the optical beam.4. The fluidic cell of claim ...

Acoustically ejecting a droplet of fluid from a reservoir by an acoustic fluid ejection apparatus

The invention provides apparatuses and methods for acoustically ejecting the fluid from a reservoir contained in or disposed on a substrate. The reservoir has a portion adapted to contain a fluid, and an acoustic radiation generator is positioned in acoustic coupling relationship to the reservoir. Acoustic radiation generated by the acoustic radiation generator is transmitted through at least the portion of the reservoir to an analyzer. The analyzer is capable of determining the energy level of the transmitted acoustic radiation and raising the energy level of subsequent pulses to a level sufficient to eject fluid droplets from the reservoir. The invention is particularly suited for delivering fluid from a plurality of reservoirs in an accurate and efficient manner.

Method For Dispensing Drops of Different Volumes

A method that allows a user to dispense a desired volume of solution from an acoustic dispensing apparatus by allowing the user to select the drop volume to be dispensed. A typical drop volume is in the range of one to twenty-five nanoliters. The method comprises the steps of creating two or more burst curves that give the relationship between liquid level and burst value, using data from the burst curves to create two or more calibration functions, and using data from the calibration functions to create a dispensing data set that is used to set the burst parameter required to dispense the selected drop volume. 1. A method for dispensing drops comprising:creating a first burst curve data set that relates a range of liquid levels of a source liquid to a range of burst values for dispensing one or more drops of the source liquid having a first drop volume, the burst values being related to a plurality of acoustic waves;creating a second burst curve data set that relates the range of liquid levels to the range of burst values for dispensing one or more drops of the source liquid having a second drop volume, where the second drop volume is not equal to the first drop volume;creating a first calibration function data set that relates the first drop volume to a first burst value measured at a first liquid level in the first burst curve data set, and that relates the second drop volume to a second burst value measured at the first liquid level in the second burst curve data set;creating a second calibration function data set that relates the first drop volume to a third burst value measured at a second liquid level in the first burst curve data set, and that relates the second drop volume to a fourth burst value measured at the second liquid level in the second burst curve data set; andcreating a dispensing data set from the first and second calibration function data sets that is used to calculate a first new burst value required to dispense one or more drops of the source ...

Droplet forming devices and system with differential surface properties

Devices, systems, and their methods of use, for generating droplets are provided. One or more geometric parameters of a microfluidic channel can be selected to generate droplets of a desired and predictable droplet size.

FLUID INJECTION USING ACOUSTIC WAVES

The present invention generally relates to the manipulation of fluids using acoustic waves such as surface acoustic waves. In some aspects, one fluid may be introduced into another fluid via application of suitable acoustic waves. For example, a fluid may be added or injected into another fluid by applying acoustic waves where, in the absence of the acoustic waves, the fluid cannot be added or injected, e.g., due to the interface or surface tension between the fluids. Thus, for example, a fluid may be injected into a droplet of another fluid. Other embodiments of the invention are generally directed to systems and methods for making or using such systems, kits involving such systems, or the like. 1. A method , comprising:providing a microfluidic system comprising a first microfluidic channel and a second microfluidic channel contacting the first microfluidic channel at a junction;providing a droplet of the first fluid contained by a carrying fluid in the first microfluidic channel, and a second fluid in the second microfluidic channel, wherein the first fluid and the second fluid contact each other at least partially within the junction to define a fluidic interface; andapplying acoustic waves to the interface to urge the second fluid to flow into the droplet wherein, in the absence of the acoustic waves, the second fluid is not urged to enter the droplet.2. The method of claim 1 , wherein the acoustic waves are applied to at least a portion of the junction.3. The method of any one of or claim 1 , wherein the acoustic waves have an amplitude whose magnitude varies in time.4. The method of any one of - claim 1 , wherein the acoustic waves are applied to the first fluid in a direction of flow of the first fluid.5. The method of any one of - claim 1 , wherein the acoustic waves are applied to the first fluid opposite a direction of flow of the first fluid.6. The method of any one of - claim 1 , wherein the acoustic waves are applied to the second fluid in a direction ...

METHODS AND DEVICES FOR ACOUSTOPHORETIC OPERATIONS IN POLYMER CHIPS

The invention relates to a method of performing an acoustophoretic operation, comprising the steps of: a. providing an acoustophoretic chip comprising a polymer substrate in which a microfluidic flow channel is positioned, b. providing at least one ultrasound transducer in acoustic contact with one surface of the substrate, c. actuating the at least one ultrasound transducer at a frequency f that corresponds to an acoustic resonance peak of the substrate including the microfluidic flow channel filled with a liquid suspension, and d. providing the liquid suspension in the flow channel to perform the acoustophoretic operation on the liquid suspension. The invention further relates to an acoustophoretic device, a method of producing an acoustophoretic device, and a microfluidic system comprising the acoustophoretic device. 115-. (canceled)16. A method of performing an acoustophoretic operation , comprising the steps of:(a) providing an acoustophoretic chip comprising a polymer substrate defining a microfluidic flow channel;(b) providing at least two ultrasound transducers in acoustic contact with a first surface of the substrate;(c) actuating the at least two ultrasound transducers at a frequency f that corresponds to an acoustic resonance peak of the substrate including the microfluidic flow channel filled with a liquid suspension; and(d) providing the liquid suspension in the flow channel to perform the acoustophoretic operation on the liquid suspension.17. The method according to claim 16 , wherein the acoustic resonance peak corresponds to a three-dimensional volume resonance in the substrate including the microfluidic flow channel claim 16 , which three-dimensional volume resonance cannot be described as a one- or two-dimensional resonance in the substrate.18. The method according to claim 16 , where the frequency f does not correspond to a resonance frequency of the channel alone.19. The method according to claim 16 , wherein in step (c) claim 16 , the at least ...

Microfluidic devices

A microfluidic device including: a transport channel having an inlet and an outlet; a plurality of pump loops extending along the transport channel, wherein each of the plurality of pump loops includes: a first branch, a second branch, and a first connecting section connecting the first branch and the second branch, wherein the first branch includes a first opening and the second branch includes a second opening, and wherein the first opening and the second opening are in direct fluid communication with the transport channel; an actuator positioned in the first branch; and a heater positioned to heat fluid in a portion of the pump loop.

CARTRIDGES, KITS, AND METHODS FOR AMPLIFICATION AND DETECTION OF ANALYTES

Devices, systems, and methods for detecting molecules of interest within a collected sample are described herein. In certain embodiments, self-contained sample analysis systems are disclosed, which include a reusable reader component, a disposable cartridge component, and a disposable sample collection component. The reader component may communicate with a remote computing device for the digital transmission of test protocols and test results. In various disclosed embodiments, the systems, components, and methods are configured to identify the presence, absence, and/or quantity of particular nucleic acids, proteins, or other analytes of interest, for example, in order to test for the presence of one or more pathogens or contaminants in a sample.

METHOD FOR GENERATING MICRO SAMPLES AND GENERATION CHIP

A method for generating micro samples and a generation chip. The method includes: simultaneously adding polyelectrolyte solutions with opposite charges respectively at a set flow rate to at least one pair of liquid inlet holes of pretreated generation chip of micro samples; wherein proportions of positive charges and negative charges of the polyelectrolyte solutions added to the pair of liquid inlet holes are substantively same; respectively controlling sample inlet channels through which the polyelectrolyte solutions in the liquid inlet holes enter the generation chip to be communicated with the liquid inlet holes; and controlling convergence of the polyelectrolyte solutions entering the sample inlet channels in a main channel of the generation chip, and forming in situ micro samples of a compound with a set diameter in the main channel within a set recombination time. 1. A method for generating micro samples , comprising:simultaneously adding polyelectrolyte solutions with opposite charges respectively at a set flow rate to at least one pair of liquid inlet holes of a pretreated generation chip of micro samples;wherein proportions of positive charges and negative charges of the polyelectrolyte solutions added to the pair of liquid inlet holes are substantively same;respectively controlling sample inlet channels through which the polyelectrolyte solutions in the liquid inlet holes enter the generation chip to be communicated with the liquid inlet holes; andcontrolling convergence of the polyelectrolyte solutions entering the sample inlet channels in a main channel of the generation chip, and forming in situ micro samples of a compound with a set diameter in the main channel within a set recombination time.2. The method of claim 1 , wherein longer the set recombination time is claim 1 , higher the concentration of the polyelectrolyte solutions is claim 1 , and bigger the diameter of the micro samples of a compound is.3. The method of claim 1 , wherein in response to ...

Devices, systems and methods for evaluation of hemostasis

Provided are devices, systems and methods for evaluation of hemostasis. In some embodiments, an apparatus is disclosed comprising a housing; a plurality of test chambers located in the housing, the plurality of test chambers including chambers configured for measurements via a system that interrogates one or more viscoelastic properties of test samples in the test chambers, wherein the one or more viscoelastic properties is used to characterize dynamics of coagulation and/or fibrinolysis.

Method to detect pre-symptom early cancer with enhanced accuracy

The current invention relates to the method to detect early stage pre-symptom cancer with enhanced accuracy. The claimed methods use anti-tumor agent to cause lysis of any existing cancer cells in a person, and causing release of genetic material from the lysed cancer cells into the blood stream of the person. The claimed methods further utilize a PCR method to amplify cancer gene in released generic material from a blood plasma sample of the person. The claimed methods further perform genetic sequencing on the blood plasma sample after PCR to identify existence of the cancer genes, thereby confirming existence of cancer.

ACOUSTIC DROPLET EJECTION OF NON-NEWTONIAN FLUIDS

Methods of ejecting droplets containing a non-Newtonian fluid by an acoustic droplet ejector can include applying a tone burst of focused acoustic energy to a fluid reservoir containing a non-Newtonian fluid at sufficient amplitude to effect droplet ejection according to a tone burst pattern. The tone burst pattern may include three discrete tone burst segments, the first tone burst segment having greater duration than the second and third segments, and third segment having greater duration than the second segment. The exact durations and amplitudes of the tone burst segments can be tuned to influence the ejection properties. 1. A method of ejecting a droplet from a reservoir containing a non-Newtonian fluid by an acoustic droplet ejector , the method comprising:applying focused acoustic energy in a first pattern comprising a first tone burst segment to a fluid surface of the non-Newtonian fluid to generate a leading lobe and a filament from the fluid surface without separating the filament from the leading lobe or the fluid surface; andapplying focused acoustic energy in a second pattern comprising a second tone burst segment and a third tone burst segment to the leading lobe to eject a droplet by separating the leading lobe from the filament to form the droplet.2. The method of claim 1 , wherein:the first tone burst segment has an amplitude and duration sufficient to raise the leading lobe and filament from the fluid surface; andthe second pattern comprises at least two discrete tone burst segments including a second tone burst segment and a third tone burst segment configured to separate the droplet from the filament.3. The method of claim 2 , wherein the first tone burst segment has greater duration than each one of the second and third tone burst segments claim 2 , and third tone burst segment having greater duration than the second tone burst segment.4. The method of claim 1 , wherein the filament comprises at least one bead of fluid claim 1 , and the second ...

MULTI-USE ACOUSTIC LEVITATION TRAP

Disclosed is a device, suitable for use as an acoustic resonator, including a base adapted to be coupled to at least one acoustic wave generator, a spacer including an aperture and a reflector, wherein the base includes a protruding part having a thickness t; the aperture of the spacer is complementary to the protruding part of the base; the device further includes a housing having an aperture complementary to the protruding part of the base and wherein the inner edge of the aperture has the same thickness t than the protruding part; and the housing is positioned between the spacer and the reflector, such that the thickness of the inner edge of the spacer defined the thickness of a cavity between the protruding part and the reflector. Also disclosed is a method of trapping particles in a fluid. 117-. (canceled)1812331. A device () , suitable for use as an acoustic resonator , comprising a base () adapted to be coupled to at least one acoustic wave generator , a spacer () comprising an aperture () and a reflector , wherein:{'b': 2', '21, 'the base () comprises a protruding part () having a thickness t;'}{'b': 31', '21, 'the aperture of the spacer () is complementary to the protruding part of the base ();'}{'b': 1', '4', '41', '21', '41', '21, 'the device () further comprises a housing () having an aperture () complementary to the protruding part of the base () and wherein the inner edge of the aperture () has the same thickness t than the protruding part (); and'}{'b': 4', '3', '5', '3', '21', '5, 'the housing () is positioned between the spacer () and the reflector (), such that the thickness of the inner edge of the spacer () defined the thickness of a cavity between the protruding part () and the reflector ().'}1921. The device according to claim 18 , wherein the protruding part () is axisymmetric.2067234567. The device according to claim 18 , further comprising a supporting base () and a top part () claim 18 , wherein the base () claim 18 , the spacer () claim 18 ...

Linear movement type reaction treatment apparatus and method thereof

The invention relates to a linear movement type reaction treatment apparatus and a method thereof, and an object thereof is to reliably prevent cross-contamination and to decrease a working time or a working space involved with reaction treatment. A linear movement type reaction treatment apparatus includes: a container group in which one or two or more reaction containers and two or more liquid storage portions are arranged in at least one linear shape; a dispensing head to which one or two or more dispensing tips are detachably attached and which is relatively movable between the dispensing tip and the container group; a magnetic portion which is capable of separating magnetic particles contained in a solution inside each dispensing tip; and an ultrasonic vibration device which applies an ultrasonic vibration to a sample storage portion, as sample storage portion at least one of the liquid storage portions are selected.

DISEASE DIAGNOSTIC SYSTEM AND METHOD

Infectious diseases have been sources of large-scale epidemics and pandemics resulting in millions of casualties worldwide. Detection of these biological agents normally involves several lab processes including sample preparation, nucleic acid separation and amplification, and diagnostic analysis. These steps, either performed manually or automated by high-throughput machinery, are tedious, expensive, and highly susceptible to cross-contamination. The present system is an integrated lab-on-a-device designed, developed, and tested in compatibility with a mechanical fixture for sample-to-answer biological analysis of infectious diseases. 1. A disease diagnostic system , the system comprising:a chip body;a mixing chamber formed in the chip body, the mixing chamber configured to receive a biological sample for disease diagnosis, the mixing chamber comprising magnetic beads, and a cell lysis buffer, the mixing chamber configured to receive energy to facilitate mixing in the mixing chamber to form a solution;a first pump formed in the chip body and coupled to the mixing chamber, the first pump configured to pump the solution out of the mixing chamber;a main channel formed in the chip body and coupled to the mixing chamber, the main channel configured to receive the solution pumped from the mixing chamber;a separation area formed in the main channel, the separation area configured to receive a magnet that traps the magnetic beads and ribonucleic acid (RNA) and/or deoxyribonucleic acid (DNA) molecules in the solution bound to the magnetic beads on a surface of the separation area;a waste chamber formed in the chip body coupled to the main channel downstream from the separation area, the waste chamber configured to receive solution comprising unbound RNA and/or DNA molecules;a first valve positioned between the main channel and the waste chamber configured to control flow of the solution comprising the unbound RNA and/or DNA molecules through the main channel to the waste ...

Separation Using Angled Acoustic Waves

Methods and systems for separating material from a host fluid use an acoustophoresis device. These methods and systems can deflect material (e.g., a second fluid, cells, beads or other particles, exosomes, viruses, oil droplets) in host fluid streams at high flow rates. 1. A system for separating material from a host fluid , the system comprising:a flow chamber defining a direction of mean flow;an ultrasonic transducer configured to be excited to generate an angled bulk acoustic standing wave with a wavelength and an acoustic radiation force in the flow chamber oriented at an acute angle relative to the direction of mean flow through the flow chamber, wherein the flow chamber has a minimum internal dimension that is at least 10 times the wavelength of the angled acoustic standing wave;a first inlet fluidly connected to the flow chamber;a second inlet fluidly connected to the flow chamber;a first outlet fluidly connected to the flow chamber; anda second outlet fluidly connected to the flow chamber.2. The system of claim 1 , wherein the first inlet is at least 0.1 inches from the angled bulk acoustic standing wave.3. The system of claim 1 , further comprising a first channel ending at the first inlet claim 1 , wherein the first channel has a substantially straight section extending at least 0.1 inches from the first inlet.4. The system of claim 1 , further comprising another ultrasonic transducer or a reflector opposite the at least one ultrasonic transducer.5. The system of claim 1 , further comprising an acoustically transparent material between the flow chamber and the at least one ultrasonic transducer.6. The system of claim 4 , further comprising a cooling water system in fluid connection with a wall of the flow chamber.7. The system of claim 4 , further comprising a solid material between the flow chamber and the at least one ultrasonic transducer with an acoustic impedance matching an acoustic impedance of the host fluid.813-. (canceled)14. The system of claim ...

SEQUENCING NUCLEIC ACID SEQUENCES

A method of sequencing multiple nucleic acid sequences, including: estimating a concentration of cells in a solution; and depositing a volume of the solution into a well on a well plate using an ejection device, the amount of solution selected to contain no more than a single cell.

DEVICES, SYSTEMS AND METHODS FOR EVALUATION OF HEMOSTASIS

Provided are devices, systems and methods for evaluation of hemostasis. Also provided are sound focusing assemblies. 173.-. (canceled)74. A device for evaluation of hemostasis than can be used with an interrogation device to measure at least one viscoelastic property of a test sample of blood , the device comprising:a housing;a plurality of test chambers, wherein the plurality of test chambers includes at least a first test chamber and a second test chamber that are each at least partially defined by the housing, wherein the first test chamber and the second test chamber are each designed to be interrogated to determine a hemostatic parameter of a test sample of blood that is received therein and a reagent or combination of reagents, wherein a first reagent or combination of reagents in the first test chamber is different than a second reagent or combination of reagents in the second test chamber; anda fluid pathway, wherein the fluid pathway includes an inlet, defined at least in part by the housing, wherein the fluid pathway is in communication with the first test chamber and the second test chamber to deliver a portion of the test sample to each of the first test chamber and the second test chamber, and wherein the fluid pathway includes a first port, defined at least in part by the housing, and from which a pressure gradient can be applied that causes the test sample to flow through the fluid pathway and into the test chambers,wherein at least a portion of the housing is thermally conductive to allow the test sample to be heated.75. The device of claim 74 , wherein the fluid pathway includes a portion designed to be held against a heater block to allow adjustment of a temperature of the test sample flowing through the portion.76. The device of claim 75 , wherein the device is designed to allow the test sample to reach 37° C. in the first test chamber and the second test chamber.77. The device of claim 76 , wherein the at least a portion of the housing that is ...

Fluidics Apparatus and Fluidics Substrate

A fluidics apparatus is disclosed for manipulation of at least one fluid sample, typically in the form of a droplet. The apparatus has a substrate surface with a sample manipulation zone for location of the fluid sample. A transducer arrangement such as an interdigitated electrode structure on a piezoelectric body provides surface acoustic waves at the substrate surface for manipulation of the fluid sample. The substrate surface has an arrangement of surface acoustic wave scattering elements forming a phononic crystal structure for affecting the transmission, distribution and/or behaviour of surface acoustic waves at the substrate surface. Also disclosed is a method for lysing a cell. In this method, the cell is comprised in a fluid sample contacting a substrate surface, the method comprising providing surface acoustic waves at the substrate surface, such that the cell lyses. 111-. (canceled)12. A method for lysing a cell , wherein the cell is comprised in a fluid sample contacting a substrate surface ,the method comprising providing surface acoustic waves at the substrate surface, such that the cell lyses.13. The method according to claim 12 , including the step of inducing rotational streaming in the fluid sample.14. The method according to claim 13 ,wherein said rotational streaming is induced in the fluid sample by providing the surface acoustic waves at the substrate surface asymmetrically with respect to the fluid sample.15. The method according to claim 12 ,wherein the power of the surface acoustic waves is progressively increased such that it becomes sufficiently high to lyse the cell.16. The method according to claim 12 ,wherein the surface acoustic waves are provided by a transducer arrangement.17. The method according to claim 16 ,wherein the substrate is separable from the transducer arrangement and coupled to the transducer arrangement by a coupling medium.18. The method according to claim 16 ,wherein said transducer arrangement provides an emission ...

Cartridges, kits, and methods for amplification and detection of analytes

Devices, systems, and methods for detecting molecules of interest within a collected sample are described herein. In certain embodiments, self-contained sample analysis systems are disclosed, which include a reusable reader component, a disposable cartridge component, and a disposable sample collection component. The reader component may communicate with a remote computing device for the digital transmission of test protocols and test results. In various disclosed embodiments, the systems, components, and methods are configured to identify the presence, absence, and/or quantity of particular nucleic acids, proteins, or other analytes of interest, for example, in order to test for the presence of one or more pathogens or contaminants in a sample.

Method And System For Reference-Assisted Droplet Detection, Indexing And Sorting For Assays And Diagnostics

Provided are methods and systems for reference object assisted droplet detection, indexing, and sorting in assays and diagnostics. Provided are compositions of reference objects and methods of usages. Provided are systems and exemplary modules and functions for the detection, counting, indexing, data processing, and sorting of reference objects and assay droplets in a microfluidic device. The reference objects may serve as a dynamic micro-scale positioning system for the indexing and sorting of individual assay droplets.

APPARATUS AND METHOD FOR CELL, SPORE, OR VIRUS CAPTURE AND DISRUPTION

Embodiments disclose an apparatus and methods for biological sample processing enabling isolation and enrichment of microbial or pathogenic constituents from the sample. A vessel for sample containment and extraction is further disclosed for engagement with a transducer capable of efficient sample disruption and lysis. Together these components provide a convenient and inexpensive solution for rapid sample preparation compatible with downstream analysis techniques. 1. An apparatus for sample processing comprising:a transducer that generates sonic or ultrasonic energy of a selected frequency, the transducer further comprising an interface having an at least partially tapered conical surface through which the sonic or ultrasonic energy is propagated; anda sample collector having a sidewall at least partially complementary to the interface of the transducer that couples with the interface and receives at least a portion of the sonic or ultrasonic energy generated by the transducer and propagates the sonic or ultrasonic energy into an interior compartment of the sample collector inducing sample cavitation that disrupts or lyses materials disposed within the sample collector.2. The apparatus of claim 1 , wherein the transducer further comprises a recessed portion in which the interface is disposed claim 1 , the recessed portion of the transducer dimensioned to receive at least a portion of the sample collector positioning the interface and sidewall in close proximity to thereby propagate the energy generated by the transducer into the interior compartment of the sample collector.3. The apparatus of claim 2 , wherein the sample collector is dimensioned to be removably retained in the recessed portion of the transducer with at least a portion of the sample collector sidewall abuts against the interface to efficiently propagate the energy generated by the transducer.4. The apparatus of claim 2 , wherein the sample collector comprises an inlet portion and an outlet portion ...

Integrated cartridge for sample homogenization and nucleic acid fragmentation

Integrated cartridges for sample homogenization, nucleic acid fragmentation, and nucleic acid detection are disclosed herein. The integrated cartridges include a main housing having a sample well and a detection chamber and a sonication feature coupled to and extending outwardly from the main housing. The sonication feature includes a sonication chamber for receiving a sample fluid. A fluidic path directs the sample fluid from the sample well, to the sonication chamber, and to the detection chamber. Systems for use with the integrated cartridges can include sonotrodes with openings for positioning the sonication feature and/or temperature sensors for monitoring the temperature of the sonication feature. Methods include moving the sample fluid from the sample well to the sonication feature, transmitting ultrasonic energy into the sample fluid, moving the sample fluid from the sonication feature to the detection chamber, and performing a nucleic acid detection assay within the detection chamber.

MICROFLUIDIC DEVICES, SYSTEMS, AND METHODS FOR EVALUATING TISSUE SAMPLES

A microfluidic device for evaluating a tissue sample can include a tissue chamber, a liquid inlet channel, a liquid outlet channel, and at least one of components (1)-(3). The tissue chamber can be defined by a plurality of walls, at least one of which is transparent. The liquid inlet and outlet channels can be in fluid communication with the tissue chamber. Components (1)-(3) can include: (1) a deformable membrane disposed within the tissue chamber and being configured to oscillate, upon application of pressure thereto, to mechanically compress the tissue sample; (2) first and second electrodes disposed about the tissue chamber and being configured such that delivery of electrical energy thereto creates an electrical gradient across the tissue chamber; and (3) at least one deformable wall, which partially defines the tissue chamber and is configured to stretch either uniaxially or biaxially upon application of negative pressure to the tissue chamber. 1. A microfluidic device for evaluating a tissue sample , the device comprising:a tissue chamber defined by a plurality of walls, at least one of the plurality of walls being transparent;a liquid inlet channel in fluid communication with the tissue chamber;a liquid outlet channel in fluid communication with the tissue chamber; and (1) a deformable membrane disposed within the tissue chamber and being configured to oscillate, upon application of pressure thereto, to mechanically compress the tissue sample;', '(2) first and second electrodes disposed about the tissue chamber and being configured such that delivery of electrical energy thereto creates an electrical gradient across the tissue chamber; and', '(3) at least one deformable wall, which partially defines the tissue chamber and is configured to stretch either uniaxially or biaxially upon application of negative pressure to the tissue chamber., 'at least one of the following components2. The device of claim 1 , further including at least one pressure line in fluid ...

MICROFLUIDIC DEVICES, SYSTEMS, AND METHODS FOR IMAGING TISSUE SAMPLES

One aspect of the present disclosure can include a microfluidic device for imaging a tissue sample. The device can include a tissue chamber, a liquid inlet channel, and a liquid outlet channel. The tissue chamber can be defined by a plurality of walls, at least one of which is transparent. The liquid inlet channel can be fluid communication with the tissue chamber. The liquid outlet channel can be in fluid communication with the tissue chamber. The tissue chamber can be sized and dimensioned to completely immobilize the tissue sample during imaging. 1. A microfluidic device for imaging a tissue sample , the device comprising:a tissue chamber defined by a plurality of walls, at least one of the plurality of walls being transparent;a liquid inlet channel in fluid communication with the tissue chamber; anda liquid outlet channel in fluid communication with the tissue chamber;wherein the tissue chamber is sized and dimensioned to completely immobilize the tissue sample during imaging.2. The device of claim 1 , further including first and second electrodes disposed about the tissue chamber and being configured such that delivery of electrical energy thereto creates an electrical gradient across the tissue chamber.3. The device of claim 1 , further including at least one pressure line in fluid communication with the tissue chamber.4. The device of claim 1 , wherein an interior surface of the tissue chamber includes one or more grooves to permit liquid flow around the tissue sample.5. The device of claim 1 , wherein an interior surface of the liquid inlet channel is textured to induce local turbulence in a liquid flowing therethrough.6. The device of claim 1 , further comprising at least one piezoelectric element connected to an outer surface of the transparent wall such that delivery of an oscillatory voltage to the piezoelectric element induces a vibration that propagates through the transparent surface into the tissue sample.7. The device of claim 1 , further including ...

A SYSTEM AND A METHOD FOR RESOLVING A CRYSTAL STRUCTURE OF A CRYSTAL AT ATOMIC RESOLUTION BY COLLECTING X-RAY DIFFRACTION IMAGES

A method and a system for resolving a crystal structure of a crystal at atomic resolution by collecting X-ray diffraction images. The method includes the steps: a) ejecting a droplet of fluid comprising single or multiple of crystal into an ultrasonic acoustic levitator; b) levitating the droplet of fluid with the crystal in the ultrasonic acoustic levitator; b) monitoring the position and the spinning of the droplet with a visualization apparatus; c) applying X-ray to the crystal, the X-ray stemming from an X-ray source; and d) detecting the X-ray diffraction images from the crystal irradiated by the X-ray source by an X-ray detector being capable to capture two dimensional diffraction patterns. 115-. (canceled)16. A system for resolving a crystal structure of a crystal at atomic resolution , the system comprising: i) one or a plurality of ultrasonic transducers configured to generate ultrasonic acoustic waves;', 'ii) one or a plurality of electrical sources for supplying a driving power for said ultrasonic transducer;', 'iii) a mechanical shield disposed to insulate an ultrasonic transmitting medium of said ultrasonic acoustic levitator and the levitating droplet therein from air and air turbulence in a surrounding environment; and', 'iv) at least one X-ray window;, 'a) an ultrasonic acoustic levitator configured to levitate at least one droplet of fluid containing one or a plurality of spinning crystals, said ultrasonic acoustic levitator includingb) one or a plurality of droplet ejectors for ejecting one or a plurality of the droplets of the fluid containing the crystal or crystals into said ultrasonic acoustic levitator;c) an X-ray source for generating an X-ray beam;d) an X-ray detector disposed to detect X-ray diffraction images scattered by the crystal upon irradiation by the X-ray beam;e) a beam stop disposed to stop an incident X-ray beam between the crystal and said X-ray detector; andf) a apparatus for aligning a position of the crystal in the levitating ...

Acoustic Cell Separation Techniques and Processes

Beads with functionalized material applied to them are exposed to an acoustic field to trap, retain or pass the beads. The beads may include or be free of ferro magnetic material. The beads may be biocompatible or biodegradable for a host. The size of the beads may vary over a range, and/or be heterogenous or homogenous. The composition of the beads may include high, neutral or low acoustic contrast material. The chemistry of the functionalized material may be compatible with existing processes. The acoustic field may be generated, for example, in an acoustic angled wave device or in an acoustic fluidized bed. 1. Material for use in a separation process , comprising:beads that are free of ferro-magnetic material; anda functionalized material on the beads for attracting a target biomaterial.2. A system for separating material , comprising:a device for generating an acoustic field;a plurality of beads that include biomaterial bound to each bead, the beads being retained or passed upon being presented to the acoustic field.3. The system of claim 2 , further comprising a fluidized bed operative to house the beads.4. The system of claim 2 , further comprising an acoustic angled wave chamber operative to process a flow of fluid containing the beads.5. The system of claim 2 , wherein the beads comprise acoustic beads.6. The system of claim 2 , wherein at least some of the plurality of beads are composed with an affinity for one or more of CD3 claim 2 , CD4 or CD8 receptors.7. A method for separating material claim 2 , comprising:applying a functionalized material to a plurality of beads;exposing the beads to biomaterial with an affinity for the functionalized material to permit the biomaterial to bind to the beads; andexposing the beads to an acoustic field to retain or pass the beads.8. The method of claim 7 , further comprising employing a fluidized bed to house the beads.9. The method of claim 7 , further comprising employing an acoustic angled wave chamber to process a ...

ELECTRONIC CONTROL OF FLUIDIC SPECIES

Various aspects of the present invention relate to the control and manipulation of fluidic species, for example, in microfluidic systems. In one aspect, the invention relates to systems and methods for making droplets of fluid surrounded by a liquid, using, for example, electric fields, mechanical alterations, the addition of an intervening fluid, etc. In some cases, the droplets may each have a substantially uniform number of entities therein. For example, 95% or more of the droplets may each contain the same number of entities of a particular species. In another aspect, the invention relates to systems and methods for dividing a fluidic droplet into two droplets, for example, through charge and/or dipole interactions with an electric field. The invention also relates to systems and methods for fusing droplets according to another aspect of the invention, for example, through charge and/or dipole interactions. In some cases, the fusion of the droplets may initiate or determine a reaction. In a related aspect of the invention, systems and methods for allowing fluid mixing within droplets to occur are also provided. In still another aspect, the invention relates to systems and methods for sorting droplets, e.g., by causing droplets to move to certain regions within a fluidic system. Examples include using electrical interactions (e.g., charges, dipoles, etc.) or mechanical systems (e.g., fluid displacement) to sort the droplets. In some cases, the fluidic droplets can be sorted at relatively high rates, e.g., at about 10 droplets per second or more. Another aspect of the invention provides the ability to determine droplets, or a component thereof, for example, using fluorescence and/or other optical techniques (e.g., microscopy), or electric sensing techniques such as dielectric sensing. 1. A method of producing droplet microreactors in a microfluidic system , comprising:producing a series of droplets of fluid surrounded by a liquid and flowing in a microfluidic ...

METHOD AND DEVICE FOR BIOMOLECULE PREPARATION AND DETECTION USING MAGNETIC ARRAY

An embodiment of the invention relates to a device for detecting an analyte in a sample. The device comprises a fluidic network and an integrated circuitry component. The fluidic network comprises multiple zones such as a sample zone, a cleaning zone and a detection zone. The fluidic network contains a magnetic particle and/or a signal particle. A sample containing an analyte is introduced, and the analyte interacts with the magnetic particle and/or the signal particle through affinity agents. A microcoil array or a mechanically movable permanent magnet is functionally coupled to the fluidic network, which are activatable to generate a magnetic field within a portion of the fluidic network, and move the magnetic particle from the sample zone to the detection zone. A detection element is present which detects optical or electrical signals from the signal particle, thus indicating the presence of the analyte. 1providing magnetic particles in a fluidic network comprising a plurality of fluidic zones, andactivating a microcoil array functionally coupled to the network to thereby move the magnetic particles without active fluidic movement in the plurality of the fluidic zones.. A method of moving magnetic particles comprising This application is related to “DEVICE AND METHOD FOR PARTICLE COMPLEX HANDLING” (07070-2011600), “ENZYMATIC SIGNAL GENERATION AND DETECTION OF BINDING COMPLEXES IN STATIONARY FLUIDIC CHIP” (07070-2011700), and “PROGRAMMABLE ELECTROMAGNETIC ARRAY FOR MOLECULE TRANSPORT” (07070-2011800), which are incorporated herein by reference.The embodiments of the invention relate to devices for conducting biomedical assays, methods of making such devices, and methods of detecting the presence of an analyte using such devices. More specifically, the embodiments relate to devices and methods that combine fluidic networks and magnetic microarrays with an integrated circuitry element that perform versatile and/or convenient analysis of an analyte with design ...

FLUID EJECTIONS IN NANOWELLS

A fluid ejection system, in an example, includes at least one nozzle of at least one die from which a fluid is ejected and at least one nanowell at which the at least one nozzle ejects an amount of fluid. A method of dispensing a fluid, in an example, includes addressing at least one nanowell with at least one nozzle of at least one die, and depositing a fluid in the nanowell with the at least one nozzle. 1. A fluid ejection system comprising:at least one nozzle of at least one die to eject a fluid; andat least one nanowell formed on a nanowell plate at which the at least one nozzle ejects an amount of fluid.2. The fluid ejection system of claim 1 , wherein the at least one nanowell is formed into a nanowell plate having a plurality of nanowells formed therein.3. The fluid ejection system of claim 1 , wherein the at least one nozzle of the at least one die is moved over a plurality of nanowells to deposit an amount of fluid therein.4. The fluid ejection system of claim 1 , wherein the at least one die comprises a plurality of nozzles with each of the plurality of nozzles ejecting fluid into at least one nanowell.5. The fluid ejection system of claim 1 , further comprising a plurality of nanowells wherein the nanowells are grouped into distinct arrays of nanowells.6. The fluid ejection system of claim 1 , wherein the fluid comprises one of solvent-based pharmaceutical compounds claim 1 , aqueous-based pharmaceutical compounds claim 1 , aqueous-based biomolecules comprising proteins claim 1 , enzymes claim 1 , lipids claim 1 , antibiotics claim 1 , mastermix claim 1 , primer claim 1 , DNA samples claim 1 , cells claim 1 , blood components claim 1 , surfactants claim 1 , and glycerol claim 1 , or combinations thereof.7. A method of dispensing a fluid claim 1 , comprising:addressing at least one nanowell with at least one nozzle of at least one die; anddispensing the fluid within the nanowell with the at least one nozzle.8. The method of claim 7 , wherein a plurality of ...

MICROFLUIDIC DEVICE, DRIVING METHOD THEREOF, AND MICROFLUIDIC SYSTEM

The present disclosure provides a microfluidic device, a driving method thereof and a microfluidic system. The microfluidic device includes a first substrate and a second substrate disposed opposite to each other, and a microcavity provided between the first and second substrates for accommodating droplets. The microfluidic device further includes at least one ultrasonic layer provided between the first and second substrates. The at least one ultrasonic layer includes a plurality of ultrasonic sensors configured to perform at least one of detection operation and driving operation to the droplets accommodated in the microcavity. 1. A microfluidic device , comprising a first substrate and a second substrate disposed opposite to each other , and a microcavity provided between the first and second substrates for accommodating droplets , and further comprising at least one ultrasonic layer provided between the first and second substrates , wherein ,the at least one ultrasonic layer includes a plurality of ultrasonic sensors configured to perform at least one of detection operation and driving operation to the droplets accommodated in the microcavity.2. The microfluidic device of claim 1 , wherein claim 1 ,at least one ultrasonic layer is provided between the first substrate and the microcavity, and/orat least one ultrasonic layer is provided between the second substrate and the microcavity.3. The microfluidic device of claim 2 , wherein claim 2 ,two ultrasonic layers are provided between the first substrate and the microcavity, ortwo ultrasonic layers are provided between the second substrate and the microcavity,an orthographic projection of each ultrasonic sensor in one of the two ultrasonic layers on the first substrate does not completely overlap an orthographic projection of each ultrasonic sensor in the other one of the two ultrasonic layers on the first substrate, andeach ultrasonic sensor in one of the two ultrasonic layers is configured to detect the droplets ...

MICROFLUIDIC NETWORK

An apparatus may include a first microfluidic valve coupled between a first reservoir and a fluid channel. The first microfluidic valve may include a fluid agitator to break a meniscus formed at an air-fluid interface and release fluid from the first reservoir into the fluid channel in response to an electrical signal. The apparatus may also include a second microfluidic valve coupled between a second reservoir and the fluid channel. Fluid from the first reservoir and fluid from the second reservoir mix in the fluid channel. 1. An apparatus comprising:a first microfluidic valve coupled between a first reservoir and a fluid channel, the first microfluidic valve comprising a fluid agitator to break a meniscus formed at an air-fluid interface and release fluid from the first reservoir into the fluid channel in response to an electrical signal; anda second microfluidic valve coupled between a second reservoir and the fluid channel;wherein fluid from the first reservoir and fluid from the second reservoir mix in the fluid channel.2. The apparatus of claim 1 , wherein fluid flowing into the fluid channel from the first reservoir breaks a meniscus formed at an air-fluid interface of the second microfluidic valve and releases fluid from the second reservoir into the fluid channel.3. The apparatus of claim 1 , wherein the electrical signal is a first electrical signal and the second microfluidic valve comprises another fluid agitator that breaks a meniscus formed at an air-fluid interface of the second microfluidic valve and releases fluid from the second reservoir into the fluid channel in response to one of the first electrical signal and a second electrical signal.4. The apparatus of claim 1 , further comprising a controller that provides the electrical signal.5. The apparatus of claim 4 , wherein the fluid agitator comprises a thermal inkjet resistor that heats fluid in the first microfluidic valve to vaporize a portion of fluid in the first microfluidic valve in ...

Micropipette and dividedly injectable apparatus

A micropipette is provided including at least one substrate, an inlet port through which a sample is delivered from the outside, a cavity to be poured and filled with the sample, an introduction hole, and an injection port for expelling the sample are formed on the at least one substrate. The substrate for forming the cavity is made of ceramics, a piezoelectric/electrostrictive element is provided for at least one wall surface of the substrate, and the sample moves as a laminar flow in the cavity. The volume of the cavity is changed by driving the piezoelectric/electrostrictive element to expell al certain amount of the sample in the cavity from the injection port. According to the micropipette, it is possible to form microspots with high accuracy and high speed. According to a dispenser including the micropipette, it is possible to efficiently dispense hundreds to ten thousands of different samples at one time and form microspots. Therefore, productivity is remarkably improved.

Payload delivery across cell membranes using continuous flow fluidic system

Methods, systems, processes, and apparatuses are provided for delivery across cell membranes. In one aspect, an apparatus includes a substrate including a mixing channel, a process chamber, and a dilution channel to perform delivery of the payload to across the cell membranes. In another aspect, a system includes reservoirs for a cell suspension, a delivery solution, and a stop solution connected to a pump. The system further includes an agitator, a heater, a temperature controller, and a controller to operate the system. In yet another aspect, cells in suspension are mixed with a delivery solution in a microfluidic mixing chip. The delivery solution includes a permeabilization agent to cause permeabilization of the cells, allowing delivery of a payload from the delivery solution to the cells across the cell membranes.

Method and device for ultrasonically manipulating particles within a fluid

Fluid-handling methods and devices for ultrasonic manipulation of fluid-borne particles comprise a fluid-handling manifold and an ultrasonic particle manipulator defining an ultrasonic cavity within the manifold. Fluid-borne particles introduced into the manifold are manipulated by controlling ultrasonic standing waves at the ultrasonic cavity. Cavities having non-uniform configurations, asymmetric standing waves and/or multiple ultrasonic cavities within the manifold are operative to control the movement of the fluid-borne particles, optionally including collecting and holding such particles, transferring particles through an intersection from one channel to another, etc. Solid phase extraction (SPE) particles, biological particles and other fluid-borne particles can be manipulated within the fluid-handling manifold.

Method and device for ultrasonically manipulating particles within a fluid

Fluid-handling methods and devices for ultrasonic manipulation of fluid-borne particles comprise a fluid-handling manifold and an ultrasonic particle manipulator defining an ultrasonic cavity within the manifold. Fluid-borne particles introduced into the manifold are manipulated by controlling ultrasonic standing waves at the ultrasonic cavity. Cavities having non-uniform configurations, asymmetric standing waves and/or multiple ultrasonic cavities within the manifold are operative to control the movement of the fluid-borne particles, optionally including collecting and holding such particles, transferring particles through an intersection from one channel to another, etc. Solid phase extraction (SPE) particles, biological particles and other fluid-borne particles can be manipulated within the fluid-handling manifold.

Devices, systems, and methods for acoustically -enhanced magnetophoresis

Systems and Methods for Separating Particles and/or Substances from a Sample Fluid

Systems and methods for separating particles and/or toxins from a sample fluid. A method according to one embodiment comprises simultaneously passing a sample fluid and a buffer fluid through a chamber such that a fluidic interface is formed between the sample fluid and the buffer fluid as the fluids pass through the chambers the sample fluid having particles of interest therein; applying a force to the fluids for urging the particles of interest to pass through the interface into the buffer fluid; and substantially separating the buffer fluid from the sample fluid.