HIGH SENSITIVITY PROTEIN WORKSTATION AND TECHNIQUES
(19)AUSTRALIAN PATENT OFFICE (54) Title HIGH SENSITIVITY PROTEIN WORKSTATION AND TECHNIQUES (21) Application No: 2002358377 (22) Application Date: 2002.12.11 (87) WIPONo: WO03/054505 (30) Priority Data (31) Number (32) 0104125-0 Date 2001.12.11 (33) Country SE (43) Publication Date : 2003.07.09 (43) Publication Journal Date : 2003.10.02 (71) Applicant(s) ASTRAZENECA AB (72) Inventor(s) LAURELL, Thomas; MARKO-VARGA, Gyorgy; NILSSON, Johan (H) Application NoAU2002358377 A1(19)AUSTRALIAN PATENT OFFICE (54) Title HIGH SENSITIVITY PROTEIN WORKSTATION AND TECHNIQUES (21) Application No: 2002358377 (22) Application Date: 2002.12.11 (87) WIPONo: WO03/054505 (30) Priority Data (31) Number (32) 0104125-0 Date 2001.12.11 (33) Country SE (43) Publication Date : 2003.07.09 (43) Publication Journal Date : 2003.10.02 (71) Applicant(s) ASTRAZENECA AB (72) Inventor(s) LAURELL, Thomas; MARKO-VARGA, Gyorgy; NILSSON, Johan A method of selecting and identifying bio-molecules present in a bio-sample is disclosed. The method comprises the steps of: obtaining a bio-sample; amplifying (2) the bio-molecules present in the bio-sample to improve the case of detection of said bio-molecules; separating the bio-molecules in said amplified bio-sample; depositing (3) the amplified bio-molecules on to a suitable media. Detecting means are then used to identify or detect the presence of bio-molecules in said amplified and separated sample wherein said amplification step occurs in close physical proximity to said deposition step. A device (1) for carrying out the method is also disclosed as is a protein chip library produced by the device or method. Claims What we claim is; 1) A method of selecting and identifying bio-molecules present in a bio-sample comprising the steps of: obtaining a bio-sample; amplifying (2) the bio-molecules present in the bio-sample to improve the ease of detection of said bio-molecules ; separating the bio-molecules in said amplified bio-sample; depositing (3) the amplified bio-molecules on to a suitable media; using identification or detecting means to identify or detect the presence of bio-molecules in said amplified and separated sample wherein said amplification step occurs in close physical proximity to said deposition step.
2. A method of selecting and identifying bio-molecules present in a bio-sample as claimed in claim 1 wherein said amplification step (2) occurs within 5 mm of said deposition step (3).
3 A method of selecting and identifying bio-molecules present in a bio-sample as claimed in claim 1 or 2 wherein said amplification step (2) and said deposition step (3) take place so that the volume of bio-sample between said steps is less than 0.25 micro-litres.
4. A method of selecting and identifying bio-molecules present in a bio-sample as claimed in claim 1,2 or 3 wherein the deposition step (3) is a non-contact one.
5. A method of selecting and identifying bio-molecules present in a bio-sample as claimed in any one of claims 1 to 4 wherein the method is conducted in a flow-through device (1).
6. A method of selecting and identifying bio-molecules present in a bio-sample as claimed in claim 1 to 4 wherein the device for carrying out said method is a one piece device (1). <Desc/Clms Page number 8> 7. A method of selecting and identifying bio-molecules present in a bio-sample as claimed in any one of claims 1 to 4 wherein the device for carrying out said method utilises a portion for performing said amplification step (10) and a separate portion for carrying out said deposition step (11) said portions being sandwiched together.
8.. A method of selecting and identifying bio-molecules present in a bio-sample as claimed in any one of claims 1 to 5 wherein the amplified sample in deposited (3) on a silicon plate by means of one of peizo-electric dispensing, magnetostrictive actuated dispensing or bubble-jet dispensing.
9. A method of selecting and identifying bio-molecules present in a bio-sample as claimed in any one of claims 1 to 8 wherein the amplified and separated bio sample is stored afterwards.
10. A device (1) for selecting and identifying bio-molecules present in a bio-sample comprising: means for obtaining a bio-sample; means (2) for amplifying the bio- molecules present in the bio-sample to improve the ease of detection of said bio- molecules; means for separating the bio-molecules in said amplified bio-sample; means (3) for depositing the amplified bio-molecules on to a suitable media; using identification or detecting means to identify or detect the presence of bio-molecules in said amplified and separated sample wherein said amplification means (2) and said depositing means (3) are in close physical proximity to each other.
11. A device for selecting and identifying bio-molecules present in a bio-sample as claimed in claim 10 wherein said amplification means (2) is within 5 mm of said depositing means (3). <Desc/Clms Page number 9> 12 A device for selecting and identifying bio-molecules present in a bio-sample as claimed in claim 10 or 11 wherein said amplification means (2) and said depositing means (3) are located so that the volume of bio-sample between said means is less than 0.25 micro-litres.
13 A device for selecting and identifying bio-molecules present in a bio-sample as claimed in claim 10, 11 or 12 wherein the device is a one piece device.
14 A device for selecting and identifying bio-molecules present in a bio-sample as claimed in any one of claims 10 to 13 wherein the amplification means (2,10) and the depositing means (3, 11) are sandwiched together.
15. A device for selecting and identifying bio-molecules present in a bio-sample as claimed in any one of claims 10 to 14 wherein the amplified sample is deposited on a silicon plate by means of peizo-electric deposition (3).
16. A device for selecting and identifying bio-molecules present in a bio-sample as claimed in any one of claims 10 to 15 wherein the device is a flow-through one.
17. A device for selecting and identifying bio-molecules present in a bio-sample as claimed in any one of claims 10 to 16 wherein the amplified and separated bio sample is stored afterwards.
18. A protein chip library produced by the method of; obtaining a bio-sample ; amplifying the bio-molecules present in the bio-sample to improve the ease of detection of said bio- molecules; separating the bio-molecules in said amplified bio-sample; separating the amplified bio-molecules in terms of molecular weight; using identification or detecting <Desc/Clms Page number 10> means to identify or detect the presence. of bio-molecules in said amplified and separated sample.
19. A method for preparing total protein compositions from thin sections of frozen tissue, cells and biological specimens, includes both cryostat sections and films of biomaterials as claimed in any one of claims 1 to 9.