АВТОМАТИЧЕСКАЯ СИСТЕМА И СПОСОБ ОБНАРУЖЕНИЯ ТОЧЕЧНОГО ИСТОЧНИКА БИОЛОГИЧЕСКОГО АГЕНТА

Изобретение относится к системам обнаружения биологической опасности, в особенности к системе обнаружения таких биологически опасных агентов, как возбудитель сибирской язвы, в почтовых отправлениях. Полностью автоматизированная система обнаружения биологических агентов содержит устройство для идентификации биологического агента, собирающее устройство для отбора аэрозольной пробы частиц распыленного биологического агента на одном участке контроля и устройство для концентрирования аэрозолей. Устройство для концентрирования аэрозолей предназначено для получения жидкой пробы из аэрозольной пробы. Автоматизированная система имеет автоматическое струйное устройство для хранения и переноса порции жидкой пробы в контейнер типа картриджа, автоматическую механическую систему перемещения контейнера из зоны накопления в пункт заливки жидкости струйного устройства, а затем в устройство для идентификации биологического агента. Устройство для идентификации биологического агента включает идентификатор ...

УСТРОЙСТВО ДЛЯ ЭКСТРАКЦИИ БИОМОЛЕКУЛ И СПОСОБ ЭКСТРАКЦИИ БИОМОЛЕКУЛ

Группа изобретений относится к области биохимии. Предложен способ и устройство для экстракции биомолекул. Устройство содержит вставной элемент, корпусный элемент с лизирующим буфером и выводной элемент для вывода биомолекул. При этом вставной элемент служит для прикрепления образца, где вставной элемент содержит крепежную деталь. Способ включает прикрепление содержащего отобранный биоматериал образца к вставному элементу, введение вставного элемента в корпусный элемент с лизирующим буфером и вывод экстрагированных из биоматериала биомолекул через выводной элемент. Изобретения обеспечивают упрощение процесса экстракции биомолекул из биоматериала, возможность эффективно и беспрепятственно выполнять тестирование с получением достоверных результатов тестирования за счет минимизации вреда от экстрагированной биомолекулы и экстракции с высокой концентрацией при небольшом количестве образцов. 2 н. и 14 з.п. ф-лы, 14 ил., 1 табл.

УСТРОЙСТВО ДЛЯ ЗАХВАТА БИОЛОГИЧЕСКИХ ЧАСТИЦ

Изобретение относится к области анализа биологических препаратов для медицинской диагностики. Устройство для захвата биологических частиц во взвешенном состоянии в жидкой среде, причем устройство имеет: контейнер, открытый через нижнее отверстие; фильтрующую мембрану, закрепленную на контейнере так, чтобы закрывать нижнее отверстие; и внутри контейнера: буфер, изготовленный из пористого пенопласта и имеющий плоскую поверхность, опирающуюся на фильтрующую мембрану; впитывающий блок, опирающийся на буфер и способный впитывать указанную жидкую среду, когда находится в контакте с указанной жидкой средой; и упругий элемент, выполненный с возможностью препятствовать расширению и/или перемещению впитывающего блока от нижнего отверстия контейнера. Технический результат - улучшение надежности захвата клеток. 4 н. и 13 з.п. ф-лы, 7 ил.

ЭЛЕКТРОФОРЕТИЧЕСКИЙ ЧИП И СПОСОБ БЫСТРОГО ОБНАРУЖЕНИЯ ПРИСУТСТВИЯ ЦЕЛЕВЫХ МОЛЕКУЛ НУКЛЕИНОВОЙ КИСЛОТЫ

Изобретение относится к биотехнологии, в частности представлен хранящийся при комнатной температуре электрофоретический чип для использования в способе быстрого обнаружения присутствия по меньшей мере одной целевой молекулы нуклеиновой кислоты из множества предварительно отобранных целевых молекул нуклеиновой кислоты в растворе, содержащий множество иммобилизованных, разделенных в пространстве и электрически изолированных отложений микрогеля. 2 н. и 21 з.п. ф-лы, 9 ил., 2 пр.

УСТРОЙСТВО ДЛЯ СБОРА БИОЛОГИЧЕСКОЙ ЖИДКОСТИ И МОДУЛЬ СБОРА

... группа изобретений относится к медицинской технике. Модуль сбора, адаптированный к приему пробы, содержит корпус, имеющий впуск и выпуск, сообщающиеся по текучей среде; камеру смешивания, расположенную между впуском и выпуском; стабилизатор пробы, расположенный между впуском и выпуском; и камеру сбора, расположенную между камерой смешивания и выпуском. Камера сбора включает первый деформируемый участок и второй деформируемый участок, а также участок жесткой стенки между первым деформируемым участком и вторым деформируемым участком. Камера смешивания принимает пробу и по меньшей мере часть заключенного в ней стабилизатора пробы. Раскрыты альтернативный вариант выполнения модуля сбора и устройство для сбора биологической жидкости, содержащее модуль сбора. Технический результат состоит в обеспечении дозирования пробы при минимальном риске для пациента. 3 н. и 16 з.п. ф-лы, 24 ил.

ОДНОРАЗОВЫЙ РЕАКТОР ДЛЯ ТВЕРДОФАЗНОГО ИММУНОАНАЛИЗА И СПОСОБ ТВЕРДОФАЗНОГО ИММУНОАНАЛИЗА

Одноразовый реактор предназначен для иммуноанализа в твердой фазе биологических объектов. Реактор содержит проточный корпус. Корпус заполнен носителем с активной или активированной поверхностью. На поверхность носителя нанесен иммунологический реагент. Объем носителя составляет до 600 мкл. При иммунологическом анализе исследуемый раствор пропускают через реактор. Иммунологический реагент взаимодействует с анализируемым веществом. Затем анализируемое вещество определяют. Использование изобретения позволяет ускорить и упростить процедуру анализа. 30 з.п. ф-лы, 2 ил.

ПРОТОЧНЫЕ ЯЧЕЙКИ С ПОКРЫТИЕМ ИЗ ГИДРОГЕЛЯ

УЛУЧШЕННЫЙ ПРОБООТБОРНИК РАСПЛАВЛЕННОГО МЕТАЛЛА

Изобретение относится к пробоотборнику для отбора проб из ванны расплавленного металла, в частности, расплавленного железа, содержащему несущую трубку, имеющую погружной конец; и узел пробоотборной камеры. Узел пробоотборной камеры содержит закрывающую пластину и корпус. Корпус содержит погружной конец, имеющий первое отверстие для впускного патрубка, и противоположный конец, имеющий второе отверстие для газового соединителя, первую поверхность, проходящую между погружным концом и противоположным концом. Первая поверхность имеет первое углубление вблизи погружного конца и второе углубление, причем первое углубление представляет собой зону анализа, а второе углубление представляет собой зону вентиляции. Изобретение позволяет улучшить качество пробоотборников прямого анализа и соответственно качество полученных проб. 2 н. и 12 з.п. ф-лы, 5 ил.

ПРОБООТБОРНИК И СПОСОБ ОТБОРА ПРОБ

Группа изобретений относится к пробоотборнику для отбора пробы расплавленного материала, имеющего температуру плавления свыше 600°С, к способу отбора данного материала, а также к прободержателю для расположения пробоотборника и к устройству, включающему данный пробоотборник и прободержатель. Пробоотборник (1) содержит камеру (2) для пробы (3), образованной из расплавленного материала. Пробоотборник содержит по меньшей мере один нижний охлаждающий корпус (6), по меньшей мере один верхний охлаждающий корпус (8), по меньшей мере один внутренний охлаждающий корпус (7) и по меньшей мере одну заполняемую часть. Камера (2) для проб окружена совместно по меньшей мере нижним охлаждающим корпусом (6) и внутренним охлаждающим корпусом (7) так, что по меньшей мере камера (2) для проб может быть охлаждена посредством по меньшей мере нижнего и внутреннего охлаждающих корпусов (6, 7). При этом заполняемая часть, соединенная с камерой (2) для проб, соединяется с камерой (2) для проб посредством отверстия ...

Устройство, прибор и аппаратура дл проведени анализов

... 1. Устройство (10), предназначенное для проведения анализа, при котором проба присутствует в измерительном приборе, и имеющее первую (12) и вторую (14) приемные камеры и впускной канал (16), который можно перемещать относительно первой и второй приемных камер, поочередно совмещая по мере необходимости таким путем его выходное отверстие с одной из указанных приемных камер, и в котором находится фильтр или связующее, удерживающее определенные компоненты пробы средство (18), отличающееся тем, что указанный впускной канал для поочередного совмещения его выходного отверстия с каждой приемной камерой выполнен перемещаемым вдоль прямолинейной траектории. 2. Устройство по п.1, которое выполнено в виде устройства кассетного типа. 3. Устройство по п.1 или 2, которое имеет первую деталь (20), в которой выполнены по меньшей мере первая и вторая приемные камеры (12, 14), которые сами являются оптическими камерами или в которых находятся оптические камеры, вторую деталь (30) или детали (40, 50), в которой ...

НАБОР ДЛЯ ВЗЯТИЯ И АНАЛИЗА ПРОБ, ПРОБООТБОРНИК И СПОСОБ АНАЛИЗА

ДИАГНОСТИЧЕСКАЯ СИСТЕМА

... 1. Комбинированная диагностическая система с несущим элементом, содержащая:место сбора физиологической жидкости, размещенное с обеспечением сбора физиологической жидкости, выпущенной в результате наложения на человека элемента для проникновения через мембрану,резервуар, выполненный с возможностью содержания используемой для тестирования текучей среды, иактиватор доставки текучей среды, причем активация указанного активатора приводит к выпуску текучей среды из резервуара.2. Комбинированная диагностическая система по п.1, в которой используемая для тестирования текучая среда выпускается в заранее заданное место.3. Комбинированная диагностическая система по п.2, которая дополнительно содержит используемый для тестирования элемент, размещенный в несущем элементе, причем при выпуске используемой для тестирования текучей среды она вступает в контакт с используемым для тестирования элементом.4. Комбинированная диагностическая система по п.1, в которой активатор выполнен с возможностью ручного ...

УСТРОЙСТВО ДЛЯ СБОРА БИОЛОГИЧЕСКОЙ ЖИДКОСТИ И МОДУЛЬ СБОРА

ОДНОРАЗОВЫЙ РЕАКЦИОННЫЙ СОСУД ДЛЯ ТВЕРДОФАЗНОГО ИММУНОАНАЛИЗА И СПОСОБ ИЗМЕРЕНИЯ КОМПОНЕНТОВ, КОТОРЫЕ МОГУТ БЫТЬ ОПРЕДЕЛЕНЫ ПОСРЕДСТВОМ ИММУННЫХ РЕАКЦИЙ

Изобретение относится к открытому сверху и снизу одноразовому реактору для иммунного анализа в твердой фазе не менее, чем с одним иммунологическим реагентом, нанесенным на материал носителя с активной или активированной поверхностью с такой плотностью заполнения, чтобы определяемый компонент количественно связывался (равновесие связи) с реагентом до выхода протекающей жидкости из носителя, причем объем слоя носителя составляет до 600 мкл, а скорость протекания жидкости соответственно определяется слоем носителя. Одноразовый реактор пригоден для определения компонентов, определяемых иммунными реакциями.

ВРАЩАЮЩЕЕСЯ ДИСКООБРАЗНОЕ УСТРОЙСТВО ДЛЯ СБОРА ОБРАЗЦОВ ТЕКУЧЕЙ СРЕДЫ

... 1. Устройство для сбора образцов текучей среды, содержащее:по существу дискообразный корпус, имеющий периферию;проходящий через корпус и ограниченный периферией капиллярный канал, имеющий первый конец и второй конец, причем первый конец выполнен с возможностью втягивания текучей среды в канал с помощью капиллярного эффекта;резервуар для сбора образцов, размещенный вблизи второго конца и сообщающийся по текучей среде с капиллярным каналом; иось вращения, проходящую через центр диска и по существу перпендикулярную основной поверхности дискообразного корпуса.2. Устройство для сбора образцов по п. 1, в котором устройство для сбора образцов выполнено с возможностью вращения вокруг оси вращения внутри кассеты, корпус которой содержит воздушное отверстие, сообщающееся по текучей среде с капиллярным каналом во время вращения диска в первое положение.3. Устройство для сбора образцов по п. 2, в котором воздушное отверстие размещено вблизи второго конца капиллярного канала и сообщается с ним по текучей ...

ПРОВОДЯЩЕЕ ТЕКУЧИЕ СРЕДЫ УСТРОЙСТВО И СПОСОБ СМЕШИВАНИЯ ТЕКУЧИХ СРЕД

АНАЛИТИЧЕСКАЯ ТЕСТ-ПОЛОСКА С КАПИЛЛЯРНЫМИ КАМЕРАМИ ДЛЯ ПРИЕМА ОБРАЗЦА, РАЗДЕЛЕННЫМИ ЗОНОЙ ФИЗИЧЕСКОГО БАРЬЕРА

... 1. Аналитическая тест-полоска для определения аналита в образце биологической текучей среды, содержащая:первую капиллярную камеру для приема образца;вторую капиллярную камеру для приема образца; изону физического барьера, размещенную между первой капиллярной камерой для приема образца и второй капиллярной камерой для приема, причем зона физического барьера размещена таким образом, чтобы предотвращать перетекание образца биологической текучей среды между первой капиллярной камерой для приема образца и второй капиллярной камерой для приема образца в процессе применения аналитической тест-полоски.2. Аналитическая тест-полоска по п. 1, в которой первая капиллярная камера для приема образца имеет по меньшей мере одно отверстие для нанесения образца и вторая камера для приема образца имеет по меньшей мере одно отверстие для нанесения образца, причем отверстие для нанесения образца первой капиллярной камеры для приема образца и отверстие для нанесения образца второй камеры для приема образца расположены ...

АНАЛИТИЧЕСКАЯ ТЕСТ-ПОЛОСКА С КАПИЛЛЯРНЫМИ КАМЕРАМИ ДЛЯ ПРИЕМА ОБРАЗЦА, РАЗДЕЛЕННЫМИ ЗОНАМИ РАЗРЫВА

... 1. Аналитическая тест-полоска для определения аналита в образце биологической текучей среды, содержащая:первую капиллярную камеру для приема образца;вторую капиллярную камеру для приема образца;первую зону разрыва, размещенную между первой капиллярной камерой для приема образца и второй капиллярной камерой для приема и образующую границу раздела первой капиллярной камеры для приема образца; ивторую зону разрыва, размещенную между первой капиллярной камерой для приема образца и второй капиллярной камерой для приема и образующую границу раздела второй капиллярной камеры для приема, и причем первая зона разрыва и вторая зона разрыва размещены таким образом, чтобы предотвращать перетекание образца биологической текучей среды между первой капиллярной камерой для приема образца и второй капиллярной камерой для приема образца в процессе применения аналитической тест-полоски.2. Аналитическая тест-полоска по п. 1, в которой первая капиллярная камера для приема образца имеет, по меньшей мере, одно ...

GERÄT, DAS VAKUUM ANWENDET, ZUR AUFTRENNUNG VON KÖRPERFLÜSSIGKEITEN

Vorrichtung zur Blutentnahme

Elektrochemischer Sensoraufbau

TESTGERÄT ZUR DURCHFÜHRUNG VON NUKLEINSÄURE-AMPLIFIKATIONSREAKTIONEN

PROBENTESTEINHEIT

Blasen eliminierende Struktur, Blasen eliminierendes Verfahren und Umwälzverfahren, das selbige nutzt

Vorgesehen werden eine Blasen eliminierende Struktur und ein Blasen eliminierendes Verfahren, welche Blasen in einer Flüssigkeit durch Umwälzen der Flüssigkeit eliminieren, und ein Umwälzverfahren, das die selbigen nutzt. Eine erste Rille (114), welche eine stromaufwärtige, Blasen eliminierende Rille ist, und eine zweite Rille (131), welche eine stromabwärtige, Blasen eliminierende Rille ist, sind von einer Mischvertiefung (13) abgezweigt. Nach Starten einer Ansaugung der Flüssigkeit von der Mischvertiefung (13) in die erste Rille (114) wird eine Ansaugung der Flüssigkeit von der Mischvertiefung (13) in die zweite Rille (131) gestartet, und nach Beendigung einer Ableitung der Flüssigkeit von der ersten Rille (114) in die Mischvertiefung (13) wird eine Ableitung der Flüssigkeit von der zweiten Rille (131) in die Mischvertiefung (13) beendet. Diese Operation wird wiederholt, um Blasen zu eliminieren.

BEHÄLTER FÜR FLÜSSIGE PROBEN UND BEFESTIGBARE TEST-MODULE

MULTIANALYSENPROBENTRÄGER.

FLUSSBESCHRÄNKER MIT SICHERHEITSMERKMAL

Ein Schnellprüfgerät

Ein Schnellerkennungsgerät, das dadurch gekennzeichnet ist: der zylindrische Behälter (4) verfügt über eine erste Kammer (41) und einer mit der ersten Kammer (41) verbundenen quantitativen Befestigungsöffnung (44); die Basis (8) ist für den Boden des zylindrischen Behälters (4) vorgesehen, wobei diese mit einer Einsetzungsmethode montiert wird, mit dem Ziel, die Einrichtung abzudichten, wobei die Mitte zwischen Basis (8) und dem zylindrischen Behälter (4) die zweite Kammer (81) bildet, und die zweite Kammer (81) ist mit der quantitativen Befestigungsöffnung (44) verbunden; eine Stecktafel (7) ist in der zweiten Kammer (81) installiert, dabei ist auf der Stecktafel (7) ein Teststreifen montiert; der in die quantitative Befestigungsöffnung (44) passende quantitative Stecker (6), verfügt oben über eine quantitative Nute (61) und an einem Ende über ein Schlüsselloch; dazu gibt es einen Schlüssel (1), der in das Schlüsselloch passt. Bei der vorliegenden Erfindung kann der quantitative Stecker ...

VORRICHTUNG ZUM BEHANDELN FLUESSIGER PROBEN IN PROBENBEHAELTERN

Neues PoC-Testsystem und Verfahren

Die vorliegende Erfindung betrifft ein Testsystem oder Assaysystem (Nachweissystem) und Testverfahren vorzugsweise im Gebrauch für den Point-of-Care (PoC) Bereich.

SYSTEM UND APPARAT ZUR DURCHFÜHRUNG EINES ASSAYVERFAHRENS

Messanordnung mit einem Trägerelement und einem Sensor

Messanordnung umfassend a) ein Trägerelement (14, 21, 32, 41) mit einer Längsachse (A) auf welchem ein Sensor (22, 33) zur Ermittlung einer Prozessgröße eines gasförmigen oder flüssigen Fluids angeordnet ist, und b) den Sensor (22, 33) wobei der Sensor (22, 33) einen Fluidkanal (34) aufweist, welcher sich innerhalb des Sensors (22, 33) erstreckt, und wobei das Trägerelement einen Fluidkanal aufweist, dadurch gekennzeichnet, dass das Trägerelement (14, 21, 32, 41) zur mechanischen Verbindung des Fluidkanals (15, 24, 36) des Trägerelements (14, 21, 32, 41) mit dem Fluidkanal (34) des Sensors (22, 33) eine Anbindungsschicht (38, 39) aufweist, die sich über einen Teilbereich einer Oberfläche des Trägerelements (14, 21, 32, 41) und über einen Teilbereich einer Oberfläche des Sensors (22, 33) erstreckt, wobei die Anbindungsschicht (38, 39) zumindest eine fluorierte Kunststoffverbindung aufweist, oder wobei das Trägerelement (14, 21, 32, 41) zur Verbindung des Fluidkanals (15, 24, 36) des Trägerelements ...

Verfahren zur Dosierung einer Flüssigkeit in einem nasschemischen Analysegerät zur Bestimmung eines Parameters einer Flüssigkeitsprobe

Die Erfindung betrifft ein Verfahren zur Dosierung einer Flüssigkeit in einem nasschemischen Analysegerät zur Bestimmung eines Parameters einer Flüssigkeitsprobe, bei welchem ein Messreaktor (7) mit Flüssigkeiten, vorzugsweise mit einer Flüssigkeitsprobe (FP) und mehreren zur Analyse notwendigen Reagenzien bzw. Standards befüllt wird, wobei vor der Befüllung des Messreaktors (7) ein vorgegebenes Volumen der Flüssigkeiten einzeln in einer, aus einem Dosierbehälter (4) und mindestens einer Dosiermesseinrichtung (5, 6) bestehenden Dosiereinheit (3) eingestellt wird. Bei einem Verfahren, bei welchem eine präzise Dosierung einer Flüssigkeit in dem Analysegerät möglich ist, wird beim Befüllen des als Dosierröhrchen (4) ausgebildeten Dosierbehälters mit der jeweiligen Flüssigkeit nach Auslösen der Dosiermesseinrichtung (5) ein Zusatzvolumen der Flüssigkeit in das Dosierröhrchen (4) über die Position der Dosiermesseinrichtung (5) hinaus eingebracht, wobei währenddessen und anschließend ein Status ...

Vorrichtung und Verfahren zum Aufnehmen und Handhaben einer flüssigen Probe und einer Substanz

Die Erfindung betrifft eine Vorrichtung und ein Verfahren zum Aufnehmen und Handhaben einer flüssigen Probe und einer Substanz. Die Vorrichtung weist eine Probeneinheit (1) und ein Substanzbehältnis (2) auf. Das Substanzbehältnis (2) weist ein Reservoir (3) auf, das ausgebildet ist, eine Substanz im Reservoir (3) aufzubewahren. Das Reservoir (3) besitzt außerdem eine Öffnung (4), die von einer Dichtfolie oder Dichtplatte (5) verschlossen ist, sodass die im Reservoir (3) aufbewahrte Substanz gegen die Umgebung abgedichtet ist. Die Probeneinheit (1) ist mit einem Probenaufnehmer (6) ausgestattet, der ausgebildet ist, eine flüssige Probe aufzunehmen und zu halten. Sie weist außerdem einen Öffner (7) auf, der ausgebildet ist, zumindest teilweise in die Öffnung (4) des Reservoirs (3) eingeführt zu werden und die Dichtfolie oder Dichtplatte (5) von der Öffnung (4) abzuheben. Dabei ist eine in Richtung der Dichtfolie oder Dichtplatte (5) angeordnete Fläche (8) des Öffners (7), die in die Öffnung ...

Vorrichtung zum Einsetzen in einen Rotor einer Zentrifuge, Zentrifuge und Verfahren zum fluidischen Koppeln von Kavitäten

Eine Vorrichtung zum Einsetzen in einen Rotor einer Zentrifuge weist in einer Stapelrichtung in einem Gehäuse mindestens zwei übereinander gestapelte Körper auf. Das Gehäuse ist ausgebildet ist, um in eine Halterung des Rotors der Zentrifuge eingesetzt zu werden. Bei einer bestimmungsgemäßen Aufnahme der Vorrichtung in dem Rotor der Zentrifuge, und bei einer Rotation des Rotors, ist ein Abstand eines der mindestens zwei Körper zu einer Rotationsachse des Rotors geringer, als ein Abstand eines anderen der mindestens zwei Körper zu der Rotationsachse des Rotors. Ein erster der mindestens zwei Körper weist zumindest eine erste und eine zweite Kavität auf und ein zweiter der mindestens zwei Körper weist zumindest eine erste Kavität auf. Die zwei Körper sind in dem Gehäuse beweglich zueinander angeordnet, um ansprechend auf eine Rotation des Rotors, in einer ersten Phase die erste Kavität des ersten Körpers mit der ersten Kavität des zweiten Körpers fluidisch zu koppeln und, in einer zweiten ...

Chemische Reaktionskassette und deren Verwendung

Chemische Reaktionskassette mit: einem elastischen Körper, der wenigstens einen Abschnitt der chemischen Reaktionskassette bildet, einer Mehrzahl von Kammern (21, 22, 24, 25) und Flusswegen (26, 27, 28, 29) zum Verbinden der Mehrzahl von Kammern (21, 22, 24, 25), die im Inneren eine Lösung aufnehmen, miteinander, wobei die Lösung in den Kammern (21, 22, 24, 25) und den Flusswegen (26, 27, 28, 29) durch eine externe Kraft auf den elastischen Körper von Außen bewegt oder blockiert werden, der elastische Körper in wenigstens zwei elastische Körperschichten (11, 12), die vertikal geschichtet sind, strukturiert ist und die Mehrzahl von Kammern (21, 22, 24, 25) und den Flusswegen (26, 27, 28, 29) zwischen einer oberen elastischen Körperschicht (11, 31, 41, 51) und einer unteren elastischen Körperschicht (12, 32, 42, 52) angeordnet sind, und einem Substrat (13, 33, 43, 53), der härter als der elastische Körper ist, dadurch...

Analytical test device

Apparatus 1 includes a first light emitter 2 for emitting around a first wavelength; a second light emitter 3 for emitting around a second wavelength; a photodetector 4. The two emitters 2, 3 independently illuminate photodetector 4 via optical path 7, the latter comprising a sample receiving portion 8. At portion 8, a normalised spatial intensity profile generated by the first emitter 2 is substantially equal to that generated by the second emitter 3. This may be achieved by slit, integrating sphere, beam splitters, fibre couplers, or stacked emitters where one is transparent to the wavelength emitted by the other. Emitters may be arrayed in a chessboard pattern; an image sensor may be used. In use, the emitters are alternately activated to obtain a first and second measurement and the difference is calculated by subtracting the two. This may improve signal to noise by removing background absorbance or scatter.

Analysis of liquid samples

A device for analytical determinations

Filtration device for biological samples

Fluidic cartridge for nucleic acid amplification and detection

Oocyte handling systems

Fluidic valve with contactless force transmission for pressing together stator and rotor

A fluidic valve (95) for switching between different fluid coupling states, comprises a stator (200) having at least one fluidic stator interface (204) and a rotor (202) having at least one fluidic rotor interface (206). The rotor (202) is rotatable relative to the stator (200) to switch the fluidic valve (95) between a plurality of different fluid coupling states between the fluidic stator interface (204) and the fluidic rotor interface (206). A force transmission mechanism (208) is configured for pressing the stator (200) and the rotor (202) together by a contactless force transmission to provide for a fluid tight sealing between the stator (200) and the rotor (202). The force transmission elements are preferably permanent magnets mounted in the rotor and arranged for pressing the stator and rotor together by a repelling magnetic force between the magnets. The fluidic valve may form part of a separation apparatus for separating a sample into a plurality of fractions. Also disclosed is ...

Improvements in filtration

Apparatus for testing the quality of a fluid sample

Low binding supports for improved solid-phase DNA hybridization and amplification

METHOD OF PREPARING MEASURING LIQUIDS

... 1494232 Testing liquids COMPUR-WERK GmbH & CO 27 March 1975 [8 May 1974] 13032/75 Heading G1B In the preparation in a test cuvet of a test liquid which comprises a mixture of a known volume of a reagent liquid with a known volume of a liquid substance to be tested, a known volume of the desired reagent liquid is stored in the test cuvet and using a pipet having a capillary action and a known volume, a quantity of the substance to be tested corresponding to the pipet volume is taken into said pipet by capillary action and the filled pipet is then introduced into the cuvet to produce the test liquid. A specific property of the test liquid, e.g. light permeability, is then measured. The substance to be tested may be taken from the human body, and to facilitate this, the cuvet or the inlet end of the pipet may be provided with a cutting edge to split the body, Figs. 4 and 7 (not shown). The cuvet after being charged with reagent liquid may be closed with an element in an air-tight fashion, ...

Breastmilk sample collection

Improved sampling bag with filter

Lid assembly for a sample tube, method of using the same to collect magnetic beads, and sample processing kit

A lid assembly 100 for a sample tube 30 comprises: a closure part 20 including means 24a, 24b, for removably attaching the closure part to the opening of the sample tube, and a bead-collecting platform 25 arranged to locate within or above the opening of the sample tube when the closure part is attached to the sample tube, wherein the bead-collecting platform has a bead-collecting surface 26 on one side, for coming into contact with liquid in the sample tube during use, and a magnet-receiving cavity 22 on the other side, behind the bead-collecting surface; and a magnet-bearing 10 part comprising a magnet 18, wherein the magnet is removably insertable into the magnet-receiving cavity, towards the bead-collecting surface, such that, when the magnet is present within the magnet-receiving cavity, the magnet is capable of holding magnetic beads against the bead collecting surface. A method of the lip to collect magnetic beads from a liquid, a sample tube, and device for holding a sample tube ...

Test device and sample carrier

HIV analysis method and device.

A method and a device for immunoassay analysis in general and in particular ...

Test device and sample carrier

HIV VIRAL LOAD TESTING

Apparatus for determining the presence of a contaminant in a sample of water or other fluid.

Hiv analysis method and device

Apparatus for determining the presence of a contaminant in a sample of water or other fluid

Honeycomb tube

Apparatus for determining the presence of a contaminant in a sample of water or other fluid

Honeycomb tube

Hiv analysis method and device

Test device and sample carrier

Honeycomb tube

Apparatus for determining the presence of a contaminant in a sample of water or other fluid

HIV VIRAL LOAD TESTING

APPARATUS AND METHOD FOR MIXING SAMPLES IN CONTAINERS

DEVICE WITH INTEGRATED MICRO STENCIL

HOSE CLIP FOR MEDICAL APPLICATIONS

MISCHBEHÄLTER FÜR EINE PHOTOMETRISCHE MESSEINRICHTUNG, SOWIE PHOTOMETRISCHES MESSVERFAHREN FÜR EINE PROBENFLÜSSIGKEIT

DEVICE TO THE ANALYSIS OF COMPONENTS OF A SAMPLE

DEVICES AND PROCEDURES FOR MEASURING THE STORAGE REACTION

DEVICES AND PROCEDURES FOR DECREASE, STORAGE AND TRANSPORT OF BIOLOGICAL SAMPLES

VORRICHTUNG ZUM NACHWEIS VON MIKROORGANISMEN

HYBRIDIZING DEVICE

INDEPENDENT TEST SENSOR

PROCEDURE AND DEVICE FOR THE EXECUTION OF A NUCLEIC ACID PREPARATION AND/OR AMPLIFICATION

PROCEDURE AND DEVICE FOR THE PRODUCTION OF A LIQUID LIQUID BOUNDARY SURFACE, IN PARTICULAR FOR SURFACE TENSION MEASUREMENT

MICRO TITRATION UNIT

ANORDNUNG UND VERFAHREN ZUM NACHWEIS VON MIKROORGANISMEN IN EINEM KULTURGEFÄSS

The invention relates to an arrangement, in particular hybridization arrangement (1), for specifically detecting microorganisms (2) in a culture vessel (3) with at least two regions, where in at least one first region (4) prokaryotic cells can be cultured and in one at least further region (5) capture molecules (6) for specifically detecting microorganisms (2) which can be cultured in the at least first region (4) are immobilized, and to a method for specifically detecting microorganisms (2) with an arrangement, in particular hybridization arrangement (1), in a culture vessel (3) with at least two regions by hybridization involving the following steps: a) transferring cells or cell constituents of the microorganisms (2) with target molecules from a first region (4), where prokaryotic cells grow, to the at least further region (5) where capture molecules (6) are immobilized, b) binding the target molecules to the capture molecules (6), c) labelling the bonded target molecules and d) detecting ...

TESTSET FÜR EINE PHOTOMETRISCHE MESSEINRICHTUNG UND PHOTOMETRISCHES MESSVERFAHREN FÜR EINE PROBENFLÜSSIGKEIT

The invention relates to a test set for a photometric measuring device, comprising a mixing container (1) that receives a first liquid (5) in the interior (4)thereof and comprises a closing element that can be removed from the filling opening (3) thereof, a metering container (8) containing a second fluid (13) in a closed hollow chamber (9), wherein the hollow chamber (9) is closed on one side by means of a slidable closing piston (11) and on the opposite side by means of a moveable plug (10). The metering container (8) can be inserted in the filling opening (3) of the mixing container (1) in a sealing manner. For easier addition of the sample, the metering container (8) comprises an integrated sampling device (21) that is in contact with the first fluid (5) present in the mixing container (1) after the metering container (8) has been inserted in the filling opening (3) of the mixing container (1).

BEHÄLTEREINHEIT FÜR EINE IM WESENTLICHEN FLÜSSIGE PROBE

Container unit (1) for a substantially liquid sample for concentrating particles contained in the sample, comprising a cup (2) for receiving the sample, a filter (6) for separating at least part of the sample from the particles and with a plunger connected to the filter (6) for moving the filter (6) through the sample, wherein the plunger is designed as a movable hollow plunger (4) inserted in a liquid-tight manner into the cup (2), the filter (6) being arranged on the front (5) of said plunger, and the container unit (1) has on the base of the cup (2) an opening (8) for removing at least some of the sample, or at least one observation window (29) for visually evaluating the sample.

Photometrische Messeinrichtung und photometrisches Messverfahren für eine Probenflüssigkeit

The invention relates to a test set (1) for a photometric measuring device, comprising a mixing container (2) which has a filling opening (3) and comprising a metering container (8) which can be sealingly inserted into the filling opening (3) of the mixing container (2) and which contains a liquid reagent (13) in a closed cavity (9). The cavity (9) has a closure plunger (11), which can be moved axially in the cavity (9), at a first end of the metering container (8), said closure plunger generating a specifiable filling pressure in the reagent (13), and the metering container (8) has a closure membrane (10) at a second metering container and which can be inserted into the mixing container (2). According to the invention, the closure membrane (10) is equipped with a predetermined breaking point (20) which breaks open when the filling pressure is exceeded in a defined manner as a result of an axial movement of the closure plunger (11), said predetermined breaking point (20) of the closure ...

SAMPLING DEVICE

METHOD FOR A HETEROGENEOUS ONE IMMUNOASSAY AND ARRANGEMENT FOR THIS.

PROCEDURE AND DEVICES FOR THE EXECUTION OF INVESTIGATIONS.

ANALYTIC TEST MIXTURE EQUIPMENT FOR THE EXECUTION OF SUCCESSIVE ANALYTIC REACTIONS.

ONE-WAY DEVICE FOR USE WITH CHEMICAL, IMMUNOCHEMI AND MICRO-BIOLOGICAL ANALYSES.

EQUIPMENT TO ANALYTIC REGULATIONS.

DEVICE FOR URINE INVESTIGATION.

PROCEDURE AND EQUIPMENT FOR THE INVESTIGATION OF A LIQUID SAMPLE OF MEANS OF A CONNECTION TEST.

DEVICE AND PROCEDURE FOR THE ANALYSIS OF LIQUID SAMPLES

EQUIPMENT TO WOULD BRING IN A SAMPLE

For the unique use of certain Reaktionsbehälter for the execution of chemical analyses

CARTRIDGE TO WOULD DRIVE THROUGH A CHEMICAL REACTION

PROCEDURE AND DEVICE FOR THE PREPARATION OF LIPIDI MESOPHASER MATERIALS

TEST DEVICE

DEVICE FOR SUCKING IN LIQUID SAMPLES AND A OBJEKTRÄGERANORDNUNG THE SO THAT IS USED

Method for producing a stamp for hot embossing

The present invention provides a process for producing a stamp for hot embossing (HE). The stamp can be constructed from any photo-resist epoxy that is stable at temperatures equal to the glass transition temperature (T g ) of the material to be stamped. The stamp can be used repeatedly without significant distortion of features. The stamp benefits from low relative cost, high fidelity of features in all three-dimensions and fast construction. The process for producing a stamp for hot embossing from a resist, comprising the steps of producing a seed layer L 1 from a selected photoresist polymer material, soft baking the seed layer L 1 , exposing said seed layer L 1 to initiate cross-linking and then post-exposure bake L 1 to fully cross-link it, coating the cross-linked seed layer L 1 with a second photoresist polymer layer L 2 ; soft baking the second photoresist polymer layer L 2 ; applying a mask to the top surface of the soft baked layer L 2 and illuminating the unmasked portions of the soft baked layer L 2 with UV radiation through the mask, wherein the exposed areas form the pattern of the embossing features, washing away un-exposed regions of the photoresist with a developer to leave behind a relief pattern formed in the second photoresist polymer layer L 2 , which relief pattern corresponds to a pattern in the mask.

Instrument for cassette for sample preparation

A parallel processing system for processing samples is described. In one embodiment, the parallel processing system includes an instrument interface parallel controller to control a tray motor driving system, a close-loop heater control and detection system, a magnetic particle transfer system, a reagent release system, a reagent pre-mix pumping system and a wash buffer pumping system.

Portable sample analyzer cartridge

A sample analyzer cartridge for use at a point of care of a patient such as in a doctor's office, in the home, or elsewhere in the field. By providing a removable and/or disposable cartridge with all of the needed reagents and/or fluids, the sample analyzer can be reliably used outside of the laboratory environment, with little or no specialized training.

Array element circuit and active matrix device

An active-matrix device is provided which includes a plurality of array element circuits arranged in rows and columns; a plurality of source addressing lines each shared between the array element circuits in corresponding same columns; a plurality of gate addressing lines each shared between the array element circuits in corresponding same rows; a plurality of sensor row select lines each shared between the array element circuits in corresponding same rows, wherein each of the plurality of array element circuits includes: an array element which is controlled by application of a drive voltage by a drive element; writing circuitry for writing the drive voltage to the drive element, the writing circuitry being coupled to a corresponding source addressing line and gate addressing line among the plurality of source addressing lines and gate addressing lines; and sense circuitry for sensing an impedance presented at the drive element, the sense circuitry being coupled to a corresponding sensor row select line; and a row driver and a column driver.

Multilayer Hydrodynamic Sheath Flow Structure

A microfabricated sheath flow structure for producing a sheath flow includes a primary sheath flow channel for conveying a sheath fluid, a sample inlet for injecting a sample into the sheath fluid in the primary sheath flow channel, a primary focusing region for focusing the sample within the sheath fluid and a secondary focusing region for providing additional focusing of the sample within the sheath fluid. The secondary focusing region may be formed by a flow channel intersecting the primary sheath flow channel to inject additional sheath fluid into the primary sheath flow channel from a selected direction. A sheath flow system may comprise a plurality of sheath flow structures operating in parallel on a microfluidic chip.

Microfluidic system including a bubble valve for regulating fluid flow through a microchannel

A microfluidic system includes a bubble valve for regulating fluid flow through a microchannel. The bubble valve includes a fluid meniscus interfacing the microchannel interior and an actuator for deflecting the membrane into the microchannel interior to regulate fluid flow. The actuator generates a gas bubble in a liquid in the microchannel when a sufficient pressure is generated on the membrane.

Sensor Housing and Reagent Chemistry

A sensor comprises a sensor housing, having a channel; a porous substrate, in the channel; an analysis chemistry reagent, on the porous substrate; and a nozzle, in fluid connection with the channel. The porous substrate fills a cross section of the channel, and the cross-sectional area of the channel at the porous substrate is greater than the cross-sectional area at the nozzle.

Process for manufacturing a micromechanical structure having a buried area provided with a filter

A process for manufacturing a micromechanical structure envisages: forming a buried cavity within a body of semiconductor material, separated from a top surface of the body by a first surface layer; and forming an access duct for fluid communication between the buried cavity and an external environment. The method envisages: forming an etching mask on the top surface at a first access area; forming a second surface layer on the top surface and on the etching mask; carrying out an etch such as to remove, in a position corresponding to the first access area, a portion of the second surface layer, and an underlying portion of the first surface layer not covered by the etching mask until the buried cavity is reached, thus forming both the first access duct and a filter element, set between the first access duct and the same buried cavity.

Methods and Devices for Rapid Urine Concentration

The present invention provides a device for the concentration of one or more target analytes contained in a urine sample. The device comprises a tube comprising an upper portion defining an opening for receiving the urine sample and a lower tapered portion terminating in collection reservoir. The tube contains a predetermined amount of a particulate binding agent which specifically binds the one or more target analytes and of a predetermined amount of a binding buffer. The device comprises means for seating the opening of the tube. The present invention further provides methods and kits for concentrating one or more target analytes in murine sample.

Optics collection and detection system and method

Optics collection and detection systems are provided for measuring optical signals from an array of optical sources over time. Methods of using the optics collection and detection systems are also described.

Bellows-Type Reservoir For Microsystems

The invention relates to a storage and dispensing vessel for microsystems and to a method for storing and dispensing a liquid. It is the object of the invention to be able to store a liquid with volatile and/or creeping substances in very small vessels over many months, preferably over at least one year, and to be able to dispense it automatically with comparatively little technical effort. For accomplishing this object, a bellows configured as a vessel is provided which is sealed, preferably welded, in an air-tight and liquid-tight manner once the bellows vessel has been filled. This bellows vessel at the same time serves as a dispensing vessel. The vessel can be emptied automatically with relatively simple means. For this purpose, it is, for example, clamped into a device which compresses the bellows, namely in the direction of a hollow needle—also referred to as cannula or hypodermic needle—or of a comparable means. In the process, the hollow needle or the comparable means pierces a vessel wall. The vessel is then automatically emptied via the hollow needle or the comparable means.

Single-sided continuous optoelectrowetting (sceow) device for droplet manipulation with light patterns

A single-sided continuous optoelectrowetting (SCOEW) device for manipulating droplets retained in a fluid over the SCOEW device with dynamic patterns of low intensity light, such as from a display screen, is described. A single pair of lateral electrodes are utilized for providing a lateral electric field bias, with transport motion controlled in response to projecting light through a photoconductive layer and dielectric layer adjacent to which droplets are retained. The device is configured for optically manipulating droplets having volumes spanning over five orders of magnitude, and can be configured to perform droplet dispensing, transport, splitting, merging, mixing and other droplet manipulation functions involving any of the above on a single sided surface.

Mixing device and mixing method for mixing small amounts of liquid

The invention relates to a mixing method for mixing at least one small quantity of liquid, in which a quantity of liquid is applied, in a reaction region and at least one surface sound wave is reacted with the quantity of liquid. The invention relates further to a mixing device for mixing at least one quantity of liquid for performing the method of the present invention, a use of the device, and a method of analysis for bond strengths on surfaces.

Non-polar solid inks for biomedical applications

A microfluidic device includes a first substrate, and a phase change ink deposited on a surface of the first substrate. The phase change ink includes a non-polar polymeric material and an optional colorant, wherein the phase change ink is solid at room temperature but is liquid at a jetting temperature of from about 60 to about 150° C., and a water contact angle on the deposited phase change ink is from 90° to about 175°.

Microreactors With Connectors Sealed Thereon; Their Manufacturing

The present invention deals with microfluidic devices ( 200 ) including a microreactor ( 20 ) and at least one connector ( 101 ) sealed thereon. It also deals with a method for manufacturing such microfluidic devices and to blocks of material suitable as connector.

System and method for automated generation and handling of liquid mixtures

The invention relates to a system ( 1 ) for supplying a microfluidic subsystem with liquids, comprising a first valve ( 14, 29, 46 ) and a first fluidic duct ( 10, 25, 28 ), for connecting said first valve ( 14, 29, 46 ) with said microfluidic subsystem and supplying a first liquid, and a second fluidic duct ( 11 ), for connecting with said microfluidic subsystem and supplying a second liquid characterized in that said first valve ( 14, 29, 46 ) is suitable for closing with time resolution not worse than 100 msec, and parameters of said first fluidic duct ( 10, 15, 28 ) are chosen such that the value of X 1 [Pa −1 ], defined as: X 1 [Pa −1 ]=(0.5×10 −9+1/ E 1 )(α R1 L 1 2 /A 1 ) is lower than 10 4 Pa −1 , where E 1 is the Young modulus of the material, of which said first fluidic duct ( 10, 25, 28 ) is made, L 1 is the length of the said first fluidic duct ( 10, 25, 28 ), A 1 is the surface area of the lumen of the said first fluidic duct ( 10, 25, 28 ) and α R1 is a constant characterizing the geometry of the said first fluidic duct ( 10, 25, 28 ) in an equation for the hydraulic resistance R 1 of the said first fluidic duct: R 1 =α R1 ( L 1 μ/A 1 2 ) with μ denoting the dynamic viscosity coefficient of the fluid filling the said first fluidic duct ( 10, 25, 28 ) in the measurement of R 1 . The invention relates also to a method for producing microdroplets on demand in such a system.

Body fluid analysis fixture

The present invention is intended to, in a configuration in which a liquid container for analysis sealed by a sealing member is penetrated by a penetrating member, prevent the sealing member from being unexpectedly broken to leak a liquid for analysis. Also, the present invention has: a container holder part that holds a liquid container for analysis; a penetrating member that penetrates a sealing member of the liquid container for analysis; a first moving mechanism that enables the penetrating member to be moved between a hole making position and a receding position; and a second moving mechanism that enables the penetrating member moved to the hole making position by the first moving mechanism to be moved toward the sealing member to a penetrating position where the penetrating member penetrates the sealing member.

Method and Apparatus for Collecting Biological Materials

A method and apparatus can separate and concentrate a selected component from a multi-component material. The multi-component material may include a whole sample such as adipose tissue, whole blood, or the like. The apparatus generally includes a moveable piston positioned within a separation container and a withdrawal tube that is operable to interact with a distal end of the collection container past the piston. Material can be withdrawn through the withdrawal tube.

Centrifugal separation kit and methods for centrifugal separation using the same

A centrifugal separation kit is used for centrifugally separating whole blood and body fluid, and a method for centrifugal separation using the kit. The centrifugal separation kit is capable of easily extracting a target substance by installing an injector to a centrifugal separation tube and collecting the centrifugally separated target substance with the injector.

Platelet aggregation using a microfluidics device

A microfluidics device to provide real time monitoring of platelet aggregation of a biological sample obtained from a subject. The device comprises a channel configured for passage of the biological sample, the channel comprising a protrusion configured to induce an upstream region of shear acceleration coupled to a downstream region of shear deceleration and defining there-between a region of peak rate of shear, the downstream region of shear deceleration defining a zone of platelet aggregation. The device further comprises a platelet detection means for detecting aggregation of platelets in the zone of aggregation as a result of passage of the biological sample through the channel. Methods to assess real time platelet aggregation of a biological sample obtained from a subject are further described.

Centrifuge and separation vessel therefore

The centrifugation vessel includes an outer wall containing an interior space. A dam defines a barrier which divides the interior space into at least two regions including a catch basin defining a higher gee region and a reservoir defining a lower gee region. These regions are joined together over the dam. The dam includes a face which is preferably tapered to enable optimization of speed of separation of a sample placed within the vessel. The vessel is usable in a biological sample processing method by having the higher gee region of the vessel configured to have an elongate form and the volume optimized for collection of a higher density fraction of the sample. Supply and withdrawal tubes extend into the regions for reliable extraction and separate collection of differing density fractions after separation by centrifugation.

Apparatus for high-throughput suspension measurements

A high-throughput optical suspension characterization instrument is disclosed, which can include hydraulically separate and at least partially transparent sample containers. A selection mechanism is operative to selectively direct light from a light source ( 12 ) through different ones of the sample containers along an optical axis, and an off-axis scattering detector ( 38,24 ) is responsive to scattered light from the light source after it has interacted with a sample. Phase analysis light scattering is used to determine the electrophoretic mobility and zeta potential of samples. A second instrument is disclosed, wherein all sample containers are illuminated simultaneously. Transmitted light is collected by a camera. The electrophoretic mobility and hydrodynamic size of the samples may be determined.

Electromagnetic multiplex assay biosensor

A method is provided for determining the presence of multiple different target analytes in a liquid sample using electrophoretic separation and magnetic labels within a self-contained reaction cartridge and an external magnetic sensor for detection. Magnetic labels are bound to target analytes through specific binding elements. By electrophoretic separation, the multiple different targets can be sorted according to their specific sizes and inherent molecular charges for better detection resolution and specificity. After the separation process, the target analytes are then recognized and trapped by the detection binding elements within the reaction cartridge. A magnetic field generator provides a changeable magnetic field that causes the bounded magnetic labels and target analytes to produce a resonance disruption detectable by a magnetic sensor. The sensor can provide a digital binary value to indicate whether or not a label particle is bound and that determines the presence of target analytes.

Thermal Microvalves

The movement and mixing of microdroplets through microchannels is described employing silicon-based microscale devices, comprising microdroplet transport channels, reaction regions, electrophoresis modules, and radiation detectors. The discrete droplets are differentially heated and propelled through etched channels. Electronic components are fabricated on the same substrate material, allowing sensors and controlling circuitry to be incorporated in the same device.

Filtering tube and microtube for the preparation and purification of small sample volumes

The present invention relates to spin baskets or columns used in the preparation and purification of small sample volumes. The spin baskets of the invention are particularly designed to be used with microtubes and are adapted to connect with such microtubes.

Devices and Process for Separating Plasma From a Blood Sample

The present invention pertains to a device for separating plasma from a blood sample comprising a stacked structure which is provided with a first portion including a separating member having a first surface for applying or receiving the blood sample, wherein the separating member is adapted to permit the passage of plasma but to inhibit the passage of cells, and a second portion including an absorptive member for absorbing the plasma, which has a second surface in contact with the separating member for receiving the plasma, wherein the absorptive member is adapted to generate a capillary pressure so as to draw plasma from the separating member to the absorptive member. The first portion is fixed to the backing member in a manner to be removed without destroying the absorptive member. The absorptive member is fixed to the backing member in a manner to be removed without destroying the absorptive member.

Systems and methods for collecting tear film and measuring tear film osmolarity

A sample receiving chip comprising a substrate that receives an aliquot volume of a sample fluid and a sample region of the substrate, sized such that the volume of the sample fluid is sufficient to operatively cover a portion of the sample region. The energy imparted into the sample fluid is transduced by the sample region to produce an output signal that indicates energy properties of the sample fluid. The sample receiving chip also includes a channel formed in the substrate, the channel configured to collect the aliquot volume of a sample fluid and transfer the aliquot volume of sample fluid to the sample region.

Valve for lab-on-a-chip systems, method for actuating and for producing valve

A substrate of a lab-on-a-chip system has two adjacent recesses, one serving as a flow channel and the other one being filled with an elastomer compound. In a first state, the elastomer compound and the substrate delimit the flow channel in a section. In a second state, the elastomer compound takes up the space in the recess in the substrate along a cross-section of the flow channel, thereby completely closing the flow channel. The substrate and the elastomer compound may be produced by injection molding techniques.

Microfluidic Cell Motility Assay

Certain isolated motile cells spontaneously migrate unidirectionally through a mechanically confined space, such as a microcapillary channel, in the absence of an external gradient (e.g., a chemical gradient). Assays and methods for detecting motile cells, and identifying chemical agents that inhibit cell migration, can include detecting the movement of motile cancer cells through a microcapillary channel.

Multi-task immunoaffinity device secured to a peripheral box and integrated to a capillary electrophoresis apparatus

The present invention relates to an immunoaffinity device for capturing one or more analytes present at high or low concentrations in simple or complex matrices. The device is designed as a modular, multi-task immunoaffinity device secured to a peripheral box and connected to capillary electrophoresis or liquid chromatography for the isolation, enrichment, separation and identification of polymeric macromolecules, primarily protein biomarkers. In one embodiment, two devices are coupled in-tandem to perform separately and sequentially microreactions and concentrations of proteins. In this embodiment, biological samples containing proteins can be subjected to proteolytic processing in the first device, and the resulting peptides subjected to selective purification and concentration in the second device.

Channel and method of forming channels

A device is made by forming sacrificial fibers on a substrate mold. The fibers and mold are covered with a first material. The substrate mold is removed, and the covered fibers are then removed to form channels in the first material.

Analysis reagent and analysis device having the analysis reagent carried therein

A reagent including a combination of a polyanionic compound and a bivalent cationic compound contains one substance selected from the group consisting of succinic acid, gluconic acid, alanine, glycine, valine, histidine, maltitol, and mannitol or at least one compound of the substance. A dry state of the reagent and deliquescence can be improved.

Devices for separating cells and methods of using them

The present invention refers to a device for separating cells of a defined size from a sample and to a system comprising such devices and further components. Such devices can comprise different components, such as inlet module, intermediate module, outlet module and microsieve. The present invention also refers to methods of operating such devices and uses thereof.

Methods and devices for molecular association and imaging

The present invention is directed to devices and methods for molecular association, particularly to devices and methods for hybridization of nucleic acids utilizing temperature gradients and imaging thereof. In one aspect, a molecular hybridization system generally includes a substrate having a plurality of molecular probes attached thereto, the plurality of probes being generally present in multiple copies arranged in localized formations on the surface of the substrate. The molecular hybridization system further generally includes a chamber that encloses the plurality of molecular probes such that a fluid containing sample may be applied and kept in contact with the substrate having the probes thereon. The molecular hybridization system also includes a temperature affecting system that generally produces at least one desired temperature on the surface of the substrate and in the adjacent fluid within the chamber.

Integrated Microfluidic Device and Methods

A microfluidic device for analyzing a sample of interest is provided. The microfluidic device can comprise a microfluidic device body, wherein the microfluidic device body comprises a sample preparation area, a nucleic acid amplification area, a nucleic acid analysis area, and a network of fluid channels. Each of the sample preparation area, the nucleic acid amplification area and the nucleic acid analysis area are fluidly interconnected to at least one of the other two areas by at least one of the fluid channels. Using the microfluidic device, sample preparation can be combined with amplification of a biologically active molecule, and a suitable biological sample can be provided for analysis and/or detection of a molecule of interest. The small-scale apparatus and methods provided are easier, faster, less expensive, and equally efficacious compared to larger scale equipment for the preparation and analysis of a biological sample.

Microfluidic device and hemoglobin measurement method using the same

A microfluidic device and a method for measurement of biomaterials using the same. The microfluidic device includes a microfluidic structure including: a sample chamber which receives and accommodates blood; a reagent chamber which contains a luminescent reactant; a first detection chamber which contains a first material that is positively charged; a second detection chamber which is connected to the first detection chamber, and contains a second material having a boronate moiety; and at least one channel which connects the sample chamber, the reagent chamber and the first and second detection chambers.

Apparatus and method for separating and isolating components of a biological fluid

A device for separating and isolating components of a biological fluid comprising a container for containing the fluid to be processed, a tube cap assembly for closing the container while providing filling and extraction communication therewith, a float assembly disposed within the container for funneling and controlling biological fluid flow into an inverted domed shaped isolation chamber within the float and controlling the biological fluid flow out of the isolation chamber for effecting an encapsulation or a sealed isolation of at least one component or fraction of the biological fluid flow within the isolation chamber during a centrifugation process. The device further comprising a flexible tube for connecting an extraction passageway disposed within the float assembly and an extraction valve of the tube cap assembly for allowing extraction of at least the one component or fraction encapsulated or isolated within the chamber.

Valve with material having modifiable degree of penetrability

The invention relates to a valve ( 2 ) for controlling a passage of particles from a first region ( 6 ) to a second region ( 7 ), wherein the valve ( 2 ) comprises a valve material ( 4 ) having a modifiable degree of penetrability and a valve region ( 16 ) comprising the valve material ( 4 ), wherein the valve region ( 16 ) and the valve material ( 4 ) are adapted such that the particles have to penetrate the valve material ( 4 ) if the particles pass the valve ( 2 ) for being transferred from the first region ( 6 ) to the second region ( 7 ). The degree of opening of the valve ( 2 ) can easily be controlled by modifying the degree of penetrability of the valve material ( 4 ), for example, by modifying the temperature of the valve material ( 4 ). Moreover, by penetrating the valve material ( 4 ) the particles can be separated from other elements like a fluid containing the particles.

Polymer microfluidic biochip fabrication

Provided are microfluidic devices and methods for fabricating and bonding such devices. Also provided are kits for analyzing analyte-containing samples and for lysing cells.

Device and process for isolating and cultivating live cells on a filter or extracting their genetic material

The process for isolating live cells on a filter or extracting their genetic material. The process comprises the steps of attaching, at least temporarily, a filter to a lower opening of a compartment having, in addition, an air inlet; inserting into the compartment a liquid carrying the cells; and attaching, in an impermeable manner, a needle, at least temporarily, to the compartment opening, the filter being positioned between the needle and the interior volume of the compartment. The process further comprises the steps of perforation, with the needle, of a plug of a vacuum tube with negative pressure relative to ambient pressure; and aspiration, by means of negative pressure from the vacuum tube, of the liquid through the filter, the filter retaining the cells.

Multi-Shell Microspheres With Integrated Chromatographic And Detection Layers For Use In Array Sensors

The development of miniaturized chromatographic systems localized within individual polymer microspheres and their incorporation into a bead-based cross-reactive sensor array platform is described herein. The integrated chromatographic and detection concept is based on the creation of distinct functional layers within the microspheres. In this first example of the new methodology, complexing ligands have been selectively immobilized to create “separation” layers harboring an affinity for various analytes. Information concerning the identities and concentrations of analytes may be drawn from the temporal properties of the beads' optical responses, Varying the nature of the ligand in the separation shell yields a collection of cross-reactive sensing elements well suited for use in array-based micro-total-analysis systems.

Variable Volume Mixing and Automatic Fluid Management for Programmable Microfluids

A microfluidic arrangement including a fluid channel configured to receive a first fluid from a first inlet and a second fluid from a second inlet, and a mixer connected to the fluid channel, the mixer including a mixer channel configured to receive a volume of the first fluid and a volume of the second fluid from the fluid channel, the mixer channel defining a mixer capacity, wherein the mixer is (i) configured to mix the volume of the first fluid and the volume of the second fluid in order to provide a mixture of the first fluid and the second fluid when the combined volume of the first fluid and the second fluid is less than the mixer capacity, and (ii) further configured to mix the volume of the first fluid and the volume of the second fluid in order to provide a mixture of the first fluid and the second fluid when the combined volume of the first fluid and the second fluid is equal to the mixer capacity.

System and method for microfluidic flow control

A system and method for controlling fluid flow within a microchannel includes a fluid circuit comprising a fluid outlet well and one or more fluid inlet wells, all in communication with a microchannel. A negative pressure differential is applied to the outlet well and fluid flow from an inlet well into the microchannel is controlled by opening or closing the inlet well to atmospheric pressure. To stop fluid flow from the inlet well, a negative pressure differential may be applied to the inlet well to equalize pressure between the inlet and outlet wells. By sequentially opening and closing different inlet wells to atmosphere, controlled amounts of different reagents can be serially introduced into the microchannel.

Method and Device for Purifying Nucleic Acids

The invention concerns a method for isolating and purifying nucleic acids from a sample and a device that is suitable therefore.

Methods For The Isolation, Accumulation, Characterization And/Or Identification Of Microorganisms Using A Filtration And Sample Transfer Device

The present invention is directed to methods, devises and kits for separating, accumulating, characterizing and/or identifying microorganisms known to be present or that may be present in a test sample. The method of the invention comprises optional lysing non-microorganism cells and/or particulates that may be present in a test sample, followed by a subsequent filtration step for isolation and/or accumulation of microorganisms. In one embodiment, a filtration and sample transfer device is used for isolation and/or accumulation of microorganisms by filtration. Once the microorganisms have been filtered for isolation and/or accumulation microorganisms, the isolated and/or accumulated microorganisms can be analyzing to acquire measurements for the characterization and/or identification of said microorganisms.

Liquid feeding system for microchip, sample detection device, and liquid feeding method for liquid feeding system for microchip

A liquid feeding system for a microchip performs: a first liquid feeding step in which a sample liquid in a sample liquid containing section is fed in the direction to a primary containing section via a reaction field; a second liquid feeding step in which, after the first liquid feeding step, the sample liquid is fed from the primary containing section in the direction to the reaction field; and a third liquid feeding step in which, after the second liquid feeding step, the feedings of the sample liquid from and to the reaction field and the primary containing section a rear side gas-liquid boundary face of the sample liquid in the first liquid feeding step and the front side and rear side gas-liquid boundary faces of the sample liquid in the second and third liquid feeding steps do not pass through the reaction field.

Micro-fluidic test apparatus and method

An apparatus, system, and method for determining the osmolarity of a fluid. The apparatus includes at least one micro-fluidic circuit and at least one electrical circuit disposed in communication with the micro-fluidic circuit for determining a property of a fluid contained within the at least one micro-fluidic circuit.

Biological microfluidics chip and related methods

A biological microfluidics chip ( 100 ) comprising a substrate ( 102 ), a microfluidic inlet port ( 104 ) defining an opening in a surface of the substrate ( 102 ), and a microfluidic outlet port ( 106 ) defining an opening in a surface of the substrate ( 102 ). The biological microfluidics chip ( 100 ) also comprises a plurality of wells ( 108 ) extending from a top surface ( 110 ) of the substrate, wherein each well ( 108 ) is bounded by one or more walls, and an inlet opening ( 112 ) and an outlet opening ( 114 ) are provided in a wall of each of the plurality of wells ( 108 ). An inlet microfluidic channel ( 116 ) is provided in the substrate ( 102 ) to connect the microfluidic inlet port ( 104 ) to each of the inlet openings ( 112 ) in the walls of the wells, and an outlet microfluidic channel ( 118 ) is provided in the substrate to connect each of the outlet openings ( 114 ) in the walls of the wells to the microfluidic outlet port ( 106 ).

Rapid Test Apparatus

Provided herein are methods and devices for rapid testing of solid, semi-solid, or liquid specimens, such a stool, blood, urine, saliva, or swab specimens of the cervix, urethra, nostril, throat, and for environmental testing.

Quantitative and self-calibrating chemical analysis using paper-based microfluidic systems

A method of determining the concentration of a test fluid sample using a paper-based microfluidic system having a plurality of hydrophilic testing zones, including: a) depositing said test fluid sample on at least one said testing zone; b) depositing a plurality of standard fluid samples or reactives of differing known concentrations on other said testing zones; c) introducing an indicator solution to each said test zone to thereby react with the deposited fluid sample and result in a colour intensity change which is a function of the fluid sample concentration; and d) comparing the differences in colour intensity between the test fluid sample and the standard fluid samples or reactives to thereby determine the concentration of said test fluid sample.

Luer seal for solid phase extraction columns

Disclosed herein is a luer seal for sealing a bottom outlet of each of the wells or columns in a plurality of Solid Phase Extraction (SPE) wells or columns in an array, the luer seal comprising a flexible, substantially flat mat having a top surface and a bottom surface, the top surface having portions defining a plurality of female luer slip connectors spaced for receiving an open tip of at least one of the plurality of solid phase extraction wells or columns, and sealingly fastening thereto for preventing fluid flow through the open tip. Also disclosed is a luer seal extractor for removing the luer seal from the column tips substantially simultaneously.

Microfluidic reactor system

A compact device for operatively coupling a solid planar substrate, for example a glass slide, to a microfluidic circuit and performing a reaction or reactions on organic matter bound to the face of the planar substrate. Typical reactions include binding, staining and/or labeling reactions. In use, a sealed reaction chamber is formed, the chamber enclosing the organic matter and at least a part of the solid substrate. Headspace in the sealed chamber between the solid substrate is generally of microfluidic dimensions, and diaphragm pump members are used to inject, exchange and/or mix the fluids in the chamber.

Multiple laminar flow-based particle and cellular separation with laser steering

The invention provides a method, apparatus and system for separating cellular components, and can be combined with holographic optical trapping manipulation or other forms of optical tweezing. One exemplary method includes providing a first flow having a plurality of components; providing a second flow; contacting the first flow with the second flow to provide a first separation region; and differentially sedimenting a first cellular component of the plurality of components into the second flow while concurrently maintaining a second cellular component of the plurality of components in the first flow. The second flow having the first cellular component is then differentially removed from the first flow having the second cellular component. Holographic optical traps may also be utilized in conjunction with the various flows to move selected components from one flow to another, as part of or in addition to a separation stage.

Selective bond reduction in microfluidic devices

The invention overcomes the limitations described for the bonding of structured layers by providing a method for selectively reducing the bonding of materials. In its most generic form, the invention uses a bonding technique in combination with a printing method for modifying or covering at least one portion of a surface to either fully or partially prevent localised bonding. The structuring process may act upon the layers either before or after the bonding of the layers. The invention overcomes the limitations described in the application of affinity chromatography by providing a planar substrate with discrete optical detection flow cells that contain porous material and have connecting microchannels for fluid delivery and/or removal, and a method for making the same.

Cell concentration, capture and lysis devices and methods of use thereof

The present invention provides a microfluidic devices and methods of use thereof for the concentration and capture of cells. A pulsed non-Faradic electric field is applied relative to a sample under laminar flow, which results to the concentration and capture of charged analyte. Advantageously, pulse timing is selected to avoid problems associated with ionic screening within the channel. At least one of the electrodes within the channel is coated with an insulating layer to prevent a Faradic current from flowing in the channel. Under pulsed application of a unipolar voltage to the electrodes, charged analyte within the sample is moved towards one of the electrodes via a transient electrophoretic force.

Test device, reaction apparatus and reactive test method

A test device having a micro flow channel including a reaction part where a reactant that is reactive to a tested chemical dispersed in a tested fluid is fixed, and at least one actuator for actuating the tested fluid to move in at least one of two opposite sides of the micro flow channel so as to homogenize a density distribution of the tested chemical in the tested fluid. The tested fluid is sent in the micro flow channel a plurality of times.

Apparatus and method for separating and isolating components of a biological fluid

A device for separating and isolating components of a biological fluid comprising a container for containing the fluid to be processed, a tube cap assembly for closing the container while providing filling and extraction communication therewith, a float assembly disposed within the container for funneling and controlling biological fluid flow into an inverted domed shaped isolation chamber within the float and controlling the biological fluid flow out of the isolation chamber for effecting an encapsulation or a sealed isolation of at least one component or fraction of the biological fluid flow within the isolation chamber during a centrifugation process. The device further comprising a flexible tube for connecting an extraction passageway disposed within the float assembly and an extraction valve of the tube cap assembly for allowing extraction of at least the one component or fraction encapsulated or isolated within the chamber.

Sample liquid supply device, sample liquid supply device set, and microchip set

A sample liquid supply device includes a container tip into which the sample liquid is introduced, a hollow needle provided at one end of the container tip such that a hollow part thereof communicates with inside of the container tip, and a sealing member that covers an opening from which the sample liquid is introduced, wherein the sealing member has a puncture-sealing property achieved by elastic deformation.

Process analyzer

A process analyzer for detection of an analyte in a liquid under analysis includes a base module and an exchangeable cartridge module. The exchangeable cartridge module comprises a sample taking device comprising a membrane configured to obtain a sample from the liquid under analysis. A first pump mechanism is configured to pump the sample away from the sample taking device. A second pump mechanism is configured to introduce a reagent into the sample. A measuring section is configured to perform a quantitative detection of the analyte in the sample. A degassing device is arranged downstream of the first pump mechanism and the second pump mechanism. The degassing device is configured to degas the sample.

Apparatus and methods for microfluidic applications

Non-rigid tape apparatus and fabrication methods for microfluidic processing applications such as gel electrophoresis are provided, where microfluidic processing is performed on selected areas. Parts of the tape are formed by high pressure plastic film forming. Membranes and other structures are self sealing during and after penetration by pipettes and electrical probes. Rigid exoskeleton elements protect the non-rigid parts during processing and facilitate transport of the tape.

Devices and method for enrichment and alteration of cells and other particles

The invention features devices and methods for the deterministic separation of particles. Exemplary methods include the enrichment of a sample in a desired particle or the alteration of a desired particle in the device. The devices and methods are advantageously employed to enrich for rare cells, e.g., fetal cells, present in a sample, e.g., maternal blood and rare cell components, e.g., fetal cell nuclei. The invention further provides a method for preferentially lysing cells of interest in a sample, e.g., to extract clinical information from a cellular component, e.g., a nucleus, of the cells of interest. In general, the method employs differential lysis between the cells of interest and other cells (e.g., other nucleated cells) in the sample.

Three-dimensional (3d) hydrodynamic focusing using a microfluidic device

A microfluidic device comprises inlets for a sample flow and an out-of-plane focusing sheath flow, and a curved channel section configured to receive the sample flow and out-of-plane focusing sheath and to provide hydrodynamic focusing of the sample flow in an out-of-plane direction, the out-of-plane direction being normal to a plane including the curved channel. Examples of the invention also include improved flow cytometers.

Micro mixer and microfluidic chip

There are provided a micro mixer capable of efficiently mixing at least two types of liquids in a simple structure and a microfluidic chip provided with the micro mixer. In order to achieve the object, the micro mixer includes a minute passage through which first and second liquids are caused to flow respectively, and a mixing vessel in which a liquid injecting port caused to communicate with the minute passage is provided in a bottom part, and the liquid injecting port is provided in a shifted position from a center of the bottom part in the bottom part. Moreover, the liquid injecting port may be provided in a shifted position from a center line of the mixing vessel.

Minimally invasive cytometry system with qcl inspection of single cells for cancer detection

This disclosure concerns a minimally invasive cytometry system including a handling system that presents single cells to at least one QCL laser source. The QCL laser source is configured to deliver light to a cell within the cells in order to induce vibrational bond absorption in one or more analytes within the cell. The cytometry system also includes a detection facility that detects the mid-infrared wavelength light transmitted by the cell and identifies the cell as either cancerous or non-cancerous.

Control of operation conditions within fluidic systems

The invention provides methods of controlling environmental conditions within a fluidic system, where such environmental conditions can affect the operation of the system in its desired function, and fluidic channels, devices, and systems that are used in practicing these methods. Such methods are generally directed to environmental control fluids, the movement of such fluids through these systems, and the interaction of these fluids with other components of the system, e.g., other fluids or solid components of the system.

Sample Processing Device

A floating chamber set may include a plurality of containers coupled to a frame such that each container translates vertically within a limited vertical range independent of the other containers. Each container may be perforated to allow liquid penetration.

Device and method for particle complex handling

An embodiment of the invention relates to a device for detecting an analyte in a sample. The device comprises a fluidic network and an integrated circuitry component. The fluidic network comprises a sample zone, a cleaning zone and a detection zone. The fluidic network contains a magnetic particle and/or a signal particle. A sample containing an analyte is introduced, and the analyte interacts with the magnetic particle and/or the signal particle through affinity agents. A microcoil array or a mechanically movable permanent magnet is functionally coupled to the fluidic network, which are activatable to generate a magnetic field within a portion of the fluidic network, and move the magnetic particle from the sample zone to the detection zone. A detection element is present which detects optical or electrical signals from the signal particle, thus indicating the presence of the analyte.

Methods and Microfluidic Devices for the Manipulation of Droplets in High Fidelity Polynucleotide Assembly

Methods and devices are provided for manipulating droplets on a support using surface tension properties, moving the droplets along a predetermined path and merging two droplets together enabling a number of chemical reactions. Disclosed are methods for controlling the droplets volumes. Disclosed are methods and devices for synthesizing at least one oligonucleotide having a predefined sequence. Disclosed are methods and devices for synthesizing and/or assembling at least one polynucleotide product having a predefined sequence from a plurality of different oligonucleotides having a predefined sequence. In exemplary embodiments, the methods involve synthesis and/or amplification of different oligonucleotides immobilized on a solid support, release of synthesized/amplified oligonucleotides in solution to form droplets, recognition and removal of error-containing oligonucleotides, moving or combining two droplets to allow hybridization and/or ligation between two different oligonucleotides, and further chain extension reaction following hybridization and/or ligation to hierarchically generate desired length of polynucleotide products.

Nanopore Device for Reversible Ion and Molecule Sensing or Migration

Disclosed are methods and devices for detection of ion migration and binding, utilizing a nanopipette adapted for use in an electrochemical sensing circuit. The nanopipette may be functionalized on its interior bore with metal chelators for binding and sensing metal ions or other specific binding molecules such as boronic acid for binding and sensing glucose. Such a functionalized nanopipette is comprised in an electrical sensor that detects when the nanopipette selectively and reversibly binds ions or small molecules. Also disclosed is a nanoreactor, comprising a nanopipette, for controlling precipitation in aqueous solutions by voltage-directed ion migration, wherein ions may be directed out of the interior bore by a repulsing charge in the bore.

Method for conducting multiple reactions in a single reaction tube

There is disclosed a method for conducting at least two reactions in a reaction tube, said method comprising the steps of providing at least two reaction phases within said reaction tube for allowing said reactions to occur therein, providing a separation phase that is immiscible with said two reaction phases and which is disposed therebetween, providing at least one particle capable of being coupled to a chemical species, wherein said particle is movable between said reaction phases to introduce said chemical species thereto.

Devices and methods for interfacing microfluidic devices with macrofluidic devices

The present disclosure is directed generally to devices and methods with the purpose of interfacing microfluidic devices with macrofluidic devices. Specifically, the present disclosure includes the de-

Temperature control system

For heating or cooling a sample contained in a vessel portion through a heat transfer member held in contact with the vessel portion, there is used the vessel portion, which has a part formed of an elastic member, expands and contracts for injection and discharge of the sample, is closed other than a connecting port with a channel connected to the vessel, and expands and contracts for injection and discharge of the sample. The vessel portion expands correspondingly to the injection when the sample is injected through an inflow path serving as the channel into the vessel portion contracting in a non-contacting state with the heat transfer member. A predetermined amount of sample is injected into the vessel portion so as to expand the vessel portion, and the vessel portion comes into contact with the heat transfer member. The vessel portion is heated or cooled through the heat transfer member.

Breath ketone detector

Ketoacidosis is an extreme and uncontrolled form of ketosis, which is a normal response to prolonged fasting. Embodiments of this invention test the ketone level of a patient by measuring the ketone bodies in breath condensation. Some embodiments include a device for medical testing comprising a hollow container, comprising powder mixture of sodium nitroferricyanide, ammonium sulfate and silica and a liquid including an ammonium hydroxide solution.

Device for a test strip holder, method and arrangement

For the simplified use of a test strip holder, a device having a holding or positioning device is provided, comprising a mixing chamber, a lid for closing the mixing chamber, and an obvious fluid opening for a fluid passage from the mixing chamber to the test strip holder. This allows for the test strip holder to be inserted into the device in a simple way and a sample can be prepared in the mixing chamber of the device. The device is provided with a mixing chamber that can be closed, wherein a reaction partner, for example a gold conjugate, can be dissolved in the sample for increasing the sensitivity of the test strip.

Apparatus for detecting target molecules and related methods

An apparatus for analysis of a sample and in particular of a biological sample. The apparatus contains a microfluidic chip with dies, adapted to be selectively activated or deactivated by presence of target molecules in the biological sample. The apparatus further contains a light source to emit light for illumination of the microfluidic chip and an optical filter to allow passage of the light from the dies once activated or deactivated by the presence of the target molecules. A method for pressurizing a microfluidic chip is also disclosed, where a chamber is provided, the chamber is connected with the microfluidic chip and pressure is applied to the chamber.

Capillary Force Actuator Device and Related Method of Applications

An actuator capable of generates force by leveraging the changes in capillary pressure and surface tension that result from the application of an electrical potential. The device, which will be referred to as a Capillary Force Actuator (CFA), and related methods, employs a conducting liquid bridge between two (or more) surfaces, at least one of which contains dielectric-covered electrodes, and operates according to the principles of electrowetting on dielectric.

Structures for controlling light interaction with microfluidic devices

Systems and methods for improved measurement of absorbance/transmission through fluidic systems are described. Specifically, in one set of embodiments, optical elements are fabricated on one side of a transparent fluidic device opposite a series of fluidic channels. The optical elements may guide incident light passing through the device such that most of the light is dispersed away from specific areas of the device, such as intervening portions between the fluidic channels. By decreasing the amount of light incident upon these intervening portions, the amount of noise in the detection signal can be decreased when using certain optical detection systems.

Decorative elements provided with a curled or crimped configuration at point of sale or point of use

Decorative grass and methods of providing same are disclosed wherein the decorative grass is either maintained in flattened configuration until restraint is removed and/or the decorative grass is curled and/or crimp at a point of use and/or sale.

Thermal cycling apparatus and method

A system for holding at least one of sample and reagent for analysis. The system includes a pair of parallel covers, at least one of which is light transmissive, of which pair a light transmissive cover forms a top, and of which pair the other forms a bottom. A frame is disposed between the covers to define, in relation to the covers, an interior volume. The frame and the covers are associated with one another to form a case, the case being substantially tight to liquids. A microfluidic array is disposed in the interior volume. The array includes a sheet of material having a pair of opposed surfaces, a thickness, and a plurality of through-holes running through the thickness between the surfaces, the through-holes containing at least one of sample and reagent.

Test strip construction

A test strip construction includes a test strip having a testing region bearing a chromogenic reagent to detect the presence of a substance in a fluid test sample. A supporting member is attached to one end of the test strip in a manner such that the testing region is remote form the attachment point and is free from any contact with supporting member. When dipped into the test sample the test strip can flutter away from the supporting member to allow for full contact between that the testing region and the test sample. When removed from the test sample the supporting member provides a consistent background for observation of color changes of the reagent.

System and Methods for Making and Processing Emulsions

An automated on-touch template bead preparation system is provided and includes a membrane-based emulsion generation subsystems, an emulsion PCR (ePCR) thermocycling plate and subsystem, and a continuous centrifugation emulsion breaking and templated bead collection subsystem. The emulsion generation subsystem provides uniformity in the preparation of an inverse emulsion and may be used to create large or small volume inverse emulsions rapidly and reproducibly. An emulsion-generating device is provided that can supply a continuous stream of an inverse emulsion to a thermocycling subsystem, in automated fashion. The ePCR subsystem can continuously thermocycle an inverse emulsion passed therethrough and includes static temperature zones and a consumable thermocycling plate. The continuous centrifugation subsystem can continuously break a thermally cycled inverse emulsion and collect template beads formed in the aqueous microreactor droplets of the inverse emulsion.

Microfluidic device and method of use

A microfluidic device and sensing method that utilize a resonating tube configured to have sufficient sensitivity to be capable of sensing the volume of a gas present as bubbles in a liquid or the flow rate and/or density of a gas or gas mixture flowing through the tube. The tube has a freestanding tube portion supported above a surface of a substrate so as to be capable of vibrating in a plane normal to the surface of the substrate. As a gas-containing fluid flows through an internal passage of the tube, a drive signal vibrates the freestanding tube portion at a resonant frequency thereof. Coriolis-induced deflections of the freestanding tube portion are sensed relative to the substrate to produce an output corresponding to the sensed deflections, and the drive signal and/or the output are assessed to determine the volume, density and/or flow rate of the gas of the gas-containing fluid.

Fluid handling apparatus and fluid handling system

Chip body 130 in which a through hole or concave is formed, intermediate film 140 , on one surface of which adhesive layer 150 ′ is formed and lower film 170 , on one surface of which transfer function layer 160 is formed, are prepared. Intermediate film 140 and lower film 170 are bonded together such that transfer function layer 160 is embedded in adhesive layer 150 ′ to form a laminated body. The laminated body and chip body 130 are bonded together by thermocompression to manufacture micro-chip 100.

Device and apparatus

A device ( 1 ) for carrying out a chemical or biochemical reaction and detecting the results, such as an assay to detect a target nucleic acid in a sample, said device comprising (i) a first well ( 8 ) in which a chemical or biochemical reaction such as a nucleic acid amplification reaction may be effected in a liquid phase or a receiving means for such a first well; (ii) a first channel ( 16 ) extending from the first well; (iii) a lateral flow assay device ( 32 ) arranged to receive liquid contents from said first channel, optionally by way of second well, on a bibulous membrane thereon, wherein said membrane contains elements that are able to detect the products of the chemical or biochemical reaction such as a target nucleic acid. Methods for using such devices and apparatus for carrying out assays using these are also described and claimed.

Microfluidic system and method for automated processing of particles from biological fluid

A microfluidic system for automatically depleting particles not of interest from a biological sample, comprising: a sampling module configured to receive the sample; and one or more microfluidic protein and nucleic acid depletion modules fluidically coupled to the sampling module and comprising binding agents configured to selectively bind to abundant plasma proteins or nucleic acids. A method for automatically depleting particles not of interest from a sample, comprising: receiving the sample; subjecting the sample to a force that separates at least a portion of the particles not of interest from the sample, thereby isolating at least a portion of the target component; passing the isolated target copmonent into a chamber; circulating the isolated target component in the chamber; and selectively capturing proteins or nucleic acids with binding agents within the chamber.

Magnetic sample purification

The invention relates to means for extracting magnetic particles (M) from a sample (S). The sample (S) is arranged adjacent to a liquid carrier (C), which is immiscible with it, in a configuration stable under the influence of gravity, and the magnetic particles (M) are moved by a magnetic field (B) from the sample (S) into the carrier (C). Preferably, the magnetic particles (M) are non-wetting with respect to the carrier (C) and will therefore form agglomerates in the carrier (C).

Sanitary swab collection system, microfluidic assay device, and methods for diagnostic assays

Biohazard specimen collection containers are provided with an external disposable skin, that is stripped away and discarded after the biohazardous specimen is collected, thus reducing or eliminating objectionable or dangerous residues on the outside surfaces of the container. Further, we teach that the sample collection container with external disposable skin may also serve as an integrated microfluidic biosample processing and analytical device, thereby providing a single entry, disposable assay unit, kit and system for “world-to-result” clinical diagnostic testing. These integrated assay devices are provided with synergic, multiple safe-handling features for protecting healthcare workers who handle them. The modified collection containers and analytical devices find application, for example, in PCR detection of infectious organisms or pathogenic markers collected on a swab.

Unit and Device for the Preparation of Cells and/or Particles in a Liquid and Method for Microscopic Analysis

A unit ( 10 ) for the preparation of cells contained in a liquid comprises a storage chamber ( 20 ) configured to store the liquid containing the cells and to release the stored liquid via an exit opening ( 22 ) upon the application of a predetermined external force, in particular a centrifugal force. A passage ( 30 ) adjacently arranged to the exit opening ( 22 ) has a cross-section larger than that of the exit opening ( 22 ). The wall at the transition from the exit opening ( 22 ) to the passage ( 30 ) forms an edge ( 32 ). The unit further comprises an observation member ( 50 ) for receiving the released liquid and an absorbing means ( 40 ) arranged adjacent to the observation member ( 50 ) between the passage ( 30 ) and the observation member ( 50 ). The absorbing means ( 40 ) has an aperture ( 42 ) allowing the released liquid to travel onto the observation member ( 50 ), and removes the liquid so as to leave the cells on the observation member ( 50 ) for observation.

Microfluidic device comprising microchannel where protrusions are formed on bottom surface

Disclosed is a microfluidic device comprising a microchannel through which fluid can flow. Protrusions are formed on the bottom surface of the microchannel. The microfluidic device increases detection sensitivity by improving optical characteristics and enhances the reactivity of biochemical reaction by slowing down the velocity of fluid flowing inside the microchannel.

Sample processing cartridge and method of processing and/or analysing a sample under centrifugal force

The present invention relates to a sample processing cartridge ( 10 ) for carrying out processing under centrifugal force acting in at least two directions as the orientation of cartridge relative to centrifugal force is changed, the cartridge comprising: a first cavity ( 18 ) adapted to contain a sample; and a second cavity ( 22 ) in fluid communication with the first cavity, wherein the first and second cavities are arranged such that the sample in the first cavity is moved therefrom to the second cavity as a centrifugal force acting on the cartridge is changed from a first direction ( 30 ) to a second direction ( 36 ), the first cavity is elongated perpendicular to the centrifugal force acting in the first direction, and the second cavity is more shallow than the first cavity and more extended in the direction of the centrifugal force acting in the second direction than the first cavity is extended in the direction of the centrifugal force acting in the first direction. The present invention also relates to a method of processing and/or analysing a sample under centrifugal force.

Novel Electrostatically Addressable Microvalves

A method of controlling a main fluid in a conduit using a microvalve is described. The microvalve includes a corresponding actuation aperture in an actuation aperture layer. A control fluid flows through the actuation aperture in response to an electric field applied via a charge distribution near an actuation aperture layer. In one embodiment, the electric field may adjust the opening and closing of the actuation aperture thereby controlling the flow of the control fluid. In a second embodiment, the control fluid is an electrorheological fluid where the electric field controls the viscosity of the ER fluid, thereby controlling fluid flow through the actuation aperture. In both embodiments the flow of the control fluid controls stretching of a flexible membrane into and out of the conduit, thereby controlling the flow of the main fluid by opening or closing the conduit.

Integrated modular unit including an analyte concentrator-microreactor device connected to a cartridge-cassette

The present invention relates to an immunoaffinity device for capturing one or more analytes present at high or low concentrations in simple or complex matrices. The device is designed as an integrated modular unit and connected to capillary electrophoresis or liquid chromatography for the isolation, enrichment, separation and identification of polymeric macromolecules, primarily protein biomarkers. The integrated modular unit includes an analyte-concentrator-microreaction device connected to a modified cartridge-cassette.

Microfluidic device and method of manufacturing the microfluidic device

A microfluidic device having a substrate with an array of curvilinear cavities. The substrate of the microfluidic device is preferably fabricated of a polymer such as polydimethylsiloxane. The microfluidic device is manufactured using a gas expansion molding technique.

Cartridge for conducting a chemical reaction

A cartridge for conducting a chemical reaction includes a body having at least one flow path formed therein. The cartridge also includes a reaction vessel extending from the body for holding a reaction mixture for chemical reaction and optical detection. The vessel comprises a rigid frame defining the side walls of a reaction chamber. The frame includes at least one channel connecting the flow path to the chamber. The vessel also includes flexible films or sheets attached to opposite sides of the rigid frame to form opposing major walls of the chamber. In addition, at least two of the side walls are optically transmissive and angularly offset from each to permit real-time optical detection of analyte in the reaction chamber.

Capillarity-based devices for performing chemical processes and associated systems and methods

The present technology is directed to capillarity-based devices for performing chemical processes and associated system and methods. In one embodiment, for example, a device can include a base configured to receive one or more fluids, a porous wick carried by the base portion, and a flow-metering element along the porous wick to modify a rate or volume of fluid flow along the porous wick. The porous wick can comprise a first pathway, a second pathway, and an intersection at which the first pathway and the second pathway converge. Input ends of the first and second pathways can be wettably distinct. Upon wetting of the input ends, fluid is configured to travel by capillary action along each pathway. The device may also include volume-metering features configured to automatically and independently control or modify a volume of fluid flow along one or more pathways of the porous wick.

Systems and methods for valving on a sample processing device

A system and method for valving on a sample processing device. The system can include a valve chamber, a process chamber, and a valve septum located between the valve chamber and the process chamber. The system can further include a fluid pathway in fluid communication with an inlet of the valve chamber, wherein the fluid pathway is configured to inhibit a liquid from entering the valve chamber and collecting adjacent the valve septum when the valve septum is in a closed configuration. The method can include rotating the sample processing device to exert a first force on the liquid that is insufficient to move the liquid into the valve chamber; forming an opening in the valve septum; and rotating the sample processing device to exert a second force on the liquid to move the liquid into the valve chamber.

Flow through metallic nanohole arrays

The present invention presents a device and methods of use thereof in combined electrohydrodynamic concentration and plasmonic detection of a charged species of interest using a flow-through nanohole array. The device comprises microchannels, which are linked to a substrate with arrays of through nanoholes, wherein the substrate comprises two layers, wherein one of the layers is made of insulator material and one of the layers is made of metal, whereby induction of an electric field across the nanohole array results in the species of interest concentrating inside the nanoholes and in the vicinity of the nanohole arrays. The induction of an electric field is achieved by means of an external electric field source, which is applied to the fluid containing the species of interest, resulting in electroosmotic (EO) flow. An additional pressure driven fluid flow in the microchannels, co-directional to the EO flow is applied by external means. The resulting fluid flow from the combination of the EO and pressure driven flow results in a total bulk fluid flow hereafter referred to as bulk flow (BF). The local electric field strength across the insulator layer of the nanoholes is high and the charged species in the fluid may exhibit a high electrophoretic (EP) velocity, opposing the BF. The local field strength in the metallic portion of the nanoholes is null, due to the conducting nature of the metal, and the charged species in the fluid exhibits a null EP velocity in this region. The BF and the EP velocity of the charged species may be balanced which may result in the concentration of the charged species inside the nanoholes and at both sides of the nanohole array. An incident light over one side of the nanohole array may result in the formation of surface plasmons (SP) at the interface of the metal and the surrounding liquid containing the concentrated species. The signal from the SP may be detected by optical means, including surface plasmon resonance (SPR) imaging and SPR ...

Microchemical nanofactories

Embodiments of an apparatus, system, and method for chemical synthesis and/or analysis are disclosed. One embodiment of a disclosed apparatus comprises a laminated, microfluidic structure defining a reactor and a separator. Such apparatuses, or portions thereof, generally have dimensions ranging from about 1 micrometer to about 100 micrometers. To implement synthetic processes, disclosed embodiments of the apparatus generally include at least one unit operation, such as a mixer, a valve, a separator, a detector, and combinations thereof. Individual apparatuses may be coupled both in series and in parallel to form a system for making chemical compounds. An individual apparatus or a system also can be used in combination with known devices and processes.

Sheath flow devices and methods

The invention relates generally to fluid processing and, in particular aspects, processing fluids for detection, selection, trapping and/or sorting of particulate moieties. Sheath flow devices described allow isolation of target species from fluid samples while avoiding non-specific binding of unwanted species to the surfaces of the separation device. Biological fluid processing, detection, sorting or selection of cells, proteins, and nucleic acids is described. The invention finds particular use in diagnostic settings, analyzing a patient's medical condition, monitoring and/or adjusting a therapeutic regimen and producing cell based products.

Particle Analysis in an Acoustic Cytometer

The present invention is a method and apparatus for acoustically manipulating one or more particles.